#alternative splicing

[bpod-bpod]

Making a Muscle

Details of the genetic and molecular mechanisms – including the control of alternative splicing (where one gene encodes multiple forms of a protein) – regulating the development of muscle revealed in fruit flies

Read the published research article here

Image adapted from work by Elena Nikonova and colleagues

Biomedical Center, Department of Physiological Chemistry, Ludwig-Maximilians-Universität München, München, Germany

Image originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)

Published in PLOS Biology, April 2024

You can also follow BPoD on Instagram, Twitter and Facebook

[medicomunicare]

L'immunità che dialoga costantemente col cervello: il ruolo segreto di C1q nel cervello che matura ed invecchia

Uno studio innovativo condotto presso il laboratorio di Beth Stevens, PhD, presso il Boston Children’s Hospital, ha rivelato che una proteina immunitaria influisce sulla sintesi proteica neuronale nel cervello che invecchia. Un precedente lavoro del laboratorio Stevens aveva scoperto che le cellule immunitarie nel sistema nervoso centrale, la microglia, aiutano a sfoltire le sinapsi nel cervello…

[fortunaestalta]

[emdotcom]

For years, I've been trying to put into words Why I give a single iota about Bendy & the Ink Machine, but it's such a tangled mess that no thread can be seperated -- they're all interwoven in a way that makes it hard to pick them out. The game, overall, makes me miserable, because I can see that there was love put into it, but a lot of it is thrown to the wayside in favor of a story that I think was retroactively improved by the sequel's recontextualizing of it, but is ultimately not worth the price of admission & majorly drops the ball.

It's easy to list things I don't like about it -- the gameplay is sparse, the combat is uninteresting, none of the chapters feel connected, the bugs that assault all my playthroughs & kill my saves are consistent & fill me with dread every time I open the game, the lack of thought in the contents of a chapter (chapter 3's wheel ""puzzle"" & the animatronic Bendy from chapter 4, in specifc, really grind my gears), which speaks to the amateurish & rushed way that the game was crafted -- there's a lot to hate, & it's easy to hate it. But I don't. Despite all that, I am compelled by this game, by what it's trying & failing & trying again to say.

It's really easy to understand why you dislike something. I couldn't have told you much about what I did like, in Ink Machine.

& then, I played Dark Revival. I didn't realize I liked the story of Ink Machine, until I played Dark Revival. It's a better made game, it's just not fucking interesting, to me, because it doesn't have a story worth tuning in to.

[coquelicoq]

i complain a lot about how difficult dumas's syntax is on my poor little brain, but for one harrowing moment i was compelled to imagine a world in which he spliced commas all over the place instead of using conjunctions and semicolons, and i realize now it could have been so, so much worse. thank you for not doing that, monsieur. i am finally learning to appreciate your writing style, 40 pages from the end of this 1400-page book. better late than never!

[kontaktlab]

From Samples to Success Discovering the Best Splice Alternatives

For music producers, access to a diverse and high-quality library of samples, presets, and MIDI packs is essential for creating exceptional tracks. While Splice has become a popular resource, several other platforms offer unique features that can enhance your music production process. This article will explore the best Splice alternatives, helping you find the perfect tools to take your music from samples to success.

Download Audiosed

1. Audiosed

Audiosed is a standout alternative to Splice, offering over 1 million premium samples, presets, and MIDI packs. Its unlimited download model allows producers to explore and use an extensive library without any credit limitations.

Key Features of Audiosed:

Unlimited Downloads: Access as many samples, presets, and MIDI packs as you need.

Diverse Library: Find sounds for any genre, from electronic to hip-hop to cinematic.

Efficient Workflow: Sync downloads directly to your desktop, organize files seamlessly, and drag-and-drop samples into your DAW.

Advanced Search and Filter: Quickly locate the perfect sounds with intuitive search and filtering tools.

Regular Updates: Access new content as it is added to the platform.

Flexible Subscription Plans: Choose from weekly, monthly, or annual subscriptions to fit your needs and budget.

Why It’s Great: Audiosed’s unlimited access model promotes limitless creativity, allowing producers to experiment freely and find the perfect sounds for their projects.

2. Loopcloud

Loopcloud offers a cloud-based sample management system, making it easy to access and integrate samples into your projects from anywhere. Its advanced features ensure that your samples fit seamlessly into your tracks.

Key Features of Loopcloud:

Cloud-Based Storage: Store and access your samples from any device.

DAW Integration: Sync samples directly with your DAW for a smooth workflow.

Automatic Key and Tempo Matching: Ensure your samples fit perfectly into your projects.

Extensive Library: Explore a vast range of samples, loops, and one-shots across various genres.

Why It’s Great: Loopcloud’s cloud-based approach and advanced matching features streamline the sample integration process, saving you time and effort.

3. LANDR Samples

LANDR Samples is part of the larger LANDR ecosystem, which includes mastering and distribution services. It offers a curated selection of high-quality, royalty-free samples across various genres.

Key Features of LANDR Samples:

Curated Collections: Access carefully selected samples and loops for guaranteed quality.

Integrated Services: Benefit from LANDR’s mastering and distribution tools for a comprehensive production workflow.

Subscription Plans: Choose a plan that suits your needs, from basic to advanced options.

Why It’s Great: LANDR’s integrated ecosystem allows you to handle multiple aspects of your production process within a single platform, streamlining your workflow from sampling to mastering.

4. Audiosed.com

Audiosed.com by Native Instruments offers a vast library of sounds and loops designed to integrate seamlessly with Native Instruments' software and hardware. This platform is ideal for producers who are already using Native Instruments products.

Key Features of Sounds.com:

Extensive Library: Access a wide range of high-quality sounds and loops.

Seamless Integration: Works perfectly with Native Instruments products, ensuring a cohesive workflow.

Subscription Options: Various plans are available to meet different production needs and budgets.

Why It’s Great: The seamless integration with Native Instruments products enhances the user experience, making it easier to incorporate new sounds into your existing setup.

5. Prime Loops

Prime Loops specializes in high-quality, cutting-edge sounds and loops across a variety of music genres. Known for their innovative and professionally produced samples, Prime Loops is a favorite among producers looking for unique sounds.

Download Audiosed

[vtgdog]

i always put it off but weaving in ends is really relaxing once i get started on it

[exhaslo]

Hiii！I love your stories!!But can you write about Miguel × hybridbunny！reader？that Miguel was a rich mafia or ceo and he bought reader from a black market or an auction.（ps：make reader sit on Miguel’s desk while he works and he ended up eating her out and fcking her hehehehehhehe）🐇🐇🐇🐇

Hehehehehehehehehehe

Warning: MINORS DNI, Smut, oral, rough sex, dirty talk, creampie

--------------------------------------------------------------------------

There were many pros and cons that came with being the CEO. Unfortunally, claiming ownership of Alchemax carried far more cons than the alternative. It did not help that Miguel took over the company from his corrupt father.

There were a lot of problems that needed to be fixed. Many were within the company itself; the associates to say the least. Miguel had to fire and clean up a lot of the corrupted associates' messes. This included having Miguel silently attend a black auction market.

Turned out, one of his former coworkers who worked in genetic splicing decided to test various animals on different people. While Miguel was so focused on Spiders that created Spiderman, his stupid coworker created hybrids and sold them on the black market.

Miguel just hoped that he could save as many people as he could. The work of tracking the others down was going to be a lot harder for him.

As Miguel sat in his VIP seat with a hood on, he watched the scum below him cheer with anticipation. None of these people cared. They just wanted new trophies. Miguel was going to buy as many hybrids were auctions and try to revert them back to regular humans.

-------

Miguel was about to lose faith. The auction was at the last bid and there had not been a single hybrid. Miguel did check and this was the only black market auction in the city. It was too dangerous for there to be anymore.

"Now! What you've all been waiting for, the most popular item during our shows! A hybrid!!" The announcer cheered.

Miguel nearly gasped, leaning forward as he watched the curtains unveil, revealing you.

"We got ourselves an adorable hybrid bunny!!! You know what they say about rabbits."

Miguel ignored the sea of laughter. You were standing on stand, shaking like a leaf. Before the announcer could even start the bid, Miguel yelled out an insane number. There were gasps in the crowd and barely anyone had the guts to go higher.

And just like that, you were bought by Miguel.

--------

You were hesitant as you followed your new 'owner' to his vehicle. The chain and collar still tight around your neck. Once you were seated in the back seat, you flinched as your tail got caught in the belt. You had to lower your ears, not wanting to hit the roof of the car.

"My apologizes, I'll get a bigger car." Miguel apologized as he entered the vehicle. You gave him a slight glare, "I'm not going to hurt you."

"Just fuck me," You whispered.

"No," Miguel sighed as he took the collar off once the car started moving, "I'm trying to right the wrongs that the former CEO of my company did."

You touched your neck, watching Miguel very carefully. You had an inkling of where this conversation was going, and it started to make your heart and body shake.

"My name is Miguel. I have no intention of using you for any purpose, but to try and undo what Alchemax did to you, if you would let me."

"Hah, so am I the lucky test subject?"

"No. I want to find all those who were experimented on. You were the first one I saved." Miguel noticed your hesitation and offered you a bottled water, "I have a room set up for you. Anything you want or need, just let me know and I'll get it for you."

"I suppose freedom isn't an option?"

"You and I both know what will happen if I let you go."

"Yeah, I know. Just wanted to hear your answer." You scoffed and leaned forward slightly, "I actually worked at Alchemax as an assistant. I don't think it will be easy to undo my DNA now that its been changed."

"I can try."

---------

It had been a few months since you were bought by Miguel. It came as a surprise, but he did mean what he said. You were living comfortably and Miguel was trying his absolute best to cure you. As you guessed, nothing worked.

That didn't stop you from wanting to help Miguel with his endeavor. Miguel had even rescued a few more hybrids during your stay with him. You couldn't help but feel a little jealous that you weren't his only one now. There was a simple reason as to why you felt like that.

You fell in love with Miguel.

You wanted to believe that Miguel liked you too, but you weren't sure what was holding him back. Perhaps guilt? Needing to see him, you started to hurry to his office. Thanks to your rabbit DNA, you were a fast runner.

"Miguel? Can I come in?" You asked with a knock at his office door.

"Of course, (Y/N)." He said with a smile, opening the door for you.

Before entering, your nose caught whiff of something delicious. Sniffing around, you ended up next to Miguel. You gently gripped his jacket, sniffing against his collar.

"(Y-Y/N), are you alright? Do I smell?" Miguel cleared his throat, careful to hold you back.

"Hm? O-Oh, sorry. You just smelled really good." You laughed nervously, wondering what was coming over you.

Miguel patted your head, assuring you that it was okay. You could only feel embarrassed again. This wasn't the first time your rabbit DNA caused you to do something silly or embarrassing. You were still having a hard time getting over making a 'secret room' in the building when winter was coming.

"Want to help me with something?" Miguel offered, motioning towards his desk.

You hurried over, taking a seat on his desk as Miguel pulled out some paperwork. Since it was hard for you to sit in regular chairs due to your tail, Miguel allowed you to have a spot on his personal desk. It felt like you were a trophy for him. One you didn't mind.

"So, what are you working on?" You asked. Miguel chuckled towards you as he leaned back in his seat,

"Company business."

"So how can I help?"

"You already are," Miguel chuckled again and leaned closer towards you, "I feel better having you next to me. I'm not as stressed."

Your cheeks started to burn up at his confession. Your heart was racing and you could feel yourself getting hot. This was bad. Lately, whenever you thought about Miguel you would get into a small frenzy, needing to relieve yourself. It had to be because of your rabbit DNA.

"(Y/N), are you alright?" Miguel asked, his hand against your forehead.

"Mhm," You winced slightly, shaking from his touch alone, "M-Miguel, I should...g-go," You stuttered, finding it hard to keep yourself together.

Miguel furrowed his brows as he gave you a quick check up. He checked your eyes, noticing the glossy lustful look, then your heart rate. Miguel inhaled deeply once he finally noticed you rub your legs together and your nipples perk.

"You're in heat?" Miguel muttered lowly, glancing at your once more, "Let me take-"

"N-No, let me stay," You whined, holding onto Miguel, nibbling against his shoulder, "I-I'm only...like this because of...of you,"

"Oh," Miguel resisted a groan, gently pushing you back, "Then, I suppose I need to take responsibility for you, huh?"

Oh, how those words turned you on even more. You whimpered and moaned against his touch as Miguel took off your pants. Your panties were soaked. You swore Miguel mumbled something under his breathe, but you were so zoned out that you couldn't hear him.

Next thing you knew, Miguel had taken your panties off and laid you back against his desk. He brought your legs around his head, making sure your tail wasn't crushed under you. His head directly in front of your vagina,

"My, my (Y/N), you should have told me sooner about your little problem. I could have helped you happily,"

"B-But-Ah~ M-Miguel~" You cried out as his tongue started to swirl against your folds.

Your eyes widen and your body arched as Miguel feasted. His tongue touching you in ways that your fingers could not. His aggressive licks and swirls against your clit causing that knot inside you to grow tighter.

"Ah~ R-Right...t-there~" You moaned, crying out your orgasm.

Miguel cleaned up your mess, his tongue now threatening to enter your drenched hole. Your whimpers and moans were delicious. He wanted to hear more, but he also didn't want to take advantage of your state. Licking your insides, Miguel hummed at your sweet taste. Your legs wrapping around his head.

'Miguel~" You whined, grinding your hips slightly.

"Now, now my little bunny, if you don't behave I won't be able to control myself," He hummed, sucking against your clit.

"P-Please...f-fuck me...I need you~" You whimpered.

Miguel felt his restraints snap. He flipped you on your stomach and inserted a finger inside your cunt. Your body shock as you moaned louder than before. Miguel groaned at how your cunt sucked his fingers in.

"Does my little bunny want to be fucked that bad? Even using your tail to seduce me." Miguel huffed, using his free hand to play with your tail.

Unable to take the pleasure, you cried out another orgasm the moment Miguel touched your tail. It was so sensitive. Pressing your face against his desk, you whimpered, begging for Miguel to fuck you. You needed him. You wanted him to make you feel good.

"Alright, I'll give my bunny what she wants."

"Mhm~ Y-Yesh," You babbled.

A sharp gasp escaped your throat as you felt Miguel's dick push through your folds. His cock stretched you out and filling you so perfectly. Your body felt so hot as his tip threaten to push your cervix. You could feel his shape every time your pussy clenched around him.

"A perfect fit. My little horny bunny likes this right?" Miguel chuckled as he started to thrust his hips into you at a rough pace, "My little horny bunny going into heat because of me."

"Ah~ Mhm~ M-Miguel~"

You swore you started to lose your common sense. Miguel was pounding the life out of your cunt and the air out of your lungs. Your vision kept blurring as you just focused on the feeling of him filling you.

You gasped as Miguel lifted your hips ever so slightly. His dick hitting your g-spot with each thrust while his free hand was playing with your tail. You were losing count how many times this man was making you cum.

"Does my little bunny want me to fill her up?" Miguel leaned over you, whispering your ear,

"Mphm~"

"I can't hear you, are you too fucked out to answer?" Miguel nibbled against your ear.

You pressed your ass up, "Inside~" You begged.

Miguel complied as proceeded to fill you with his cum. He moaned lowly, giving you a few more pumps before coming to a stop. Miguel started to pull out, but heard you whine in protest. A chuckle escaped his throat as he continued to slap his hips into you.

Miguel continued to fuck you until your heat finally died out. By the end of what seemed like endless fucking, both you and Miguel were out of breathe. Miguel had you seated against his lap, resting your body against his.

You whimpered tiredly, cum pouring out of your cunt. Miguel rubbed your back, his hand nudging against your tail slightly.

"Mhm,"

"Shh, it was an accident, baby." Miguel whispered, rubbing your upper back, "You should have told me when these heats started. I can make some medicine to help you."

"You're fine," You whispered lowly. Miguel chuckled, kissing your head,

"If that's the case, then it would be easier for you to stay at my place. I have been looking for a wife,"

"Mhm...I'll gladly...take that role," You muttered tiredly.

Miguel resisted a chuckle. He waited for you to fall asleep before dressing the two of you. He had one of his assistants bring your stuff to his place as Miguel took you home. He was going to make you as comfortable as possible.

But first, you both needed a shower.

-------------------------------------------------------------------------

Hope you enjoyed!!!!

[princip1914]

I recut the kiss from Good Omens to emphasize Aziraphale kissing back. I already posted the longer version (yes, I have made alternate cuts of my kiss alternate cut, yes I know this is ridiculous) but I realized I liked it in shorter gif form better. After the cut is the OG kiss for comparison and some notes on my process.

Edited Kiss:

Original Kiss:

On the longer version, I had some people ask what I changed. Essentially, what stood out to me when I saw the original version of the kiss was that it was just so...choppy. Particularly, the cut right before they break apart where we go from Aziraphale's hand on Crowley's back to him waving it around in the air, just didn't look natural to me. The original version seems to splice together several takes of a shorter kiss, with some pieces of the kiss out of order from how it was probably acted:

In the first cut of the sequence we see Aziraphale from over Crowley's shoulder and his eyes are open and he looks shocked and uncomfortable, then he relaxes into the kiss a bit and closes his eyes and his hand comes up to Crowely's shoulder and caresses it. My guess is this is the sequence and duration of the kiss from start to (nearly) finish as it was blocked and acted. But the kiss as it appears in the show is much longer than the acted kiss. From that first cut of Aziraphale's face seen over Crowely's shoulder we go to:

A wide cut where Aziraphale is waving his arm in the air and looks uncomfortable--likely filmed at the start of the kiss from a different angle and then spliced in.

A close up of Aziraphale stroking Crowley's back which seems to me to come from the end of the kiss, once he has relaxed into it. (This is the one all the girlies are gif-ing and yeah, it's a great shot so I'm glad the fandom has gone wild for it!)

Another shot from the relaxed bit of the kiss which is a close up on their faces with Aziraphale leaning in a bit

The last shot of the kiss where Aziraphale's arm has come off Crowely's back again and he's waving it in the air and looking uncomfortable as Crowley pulls away. I think this shot may actually also be from the start of the kiss, or it is actually how the end of the kiss was blocked out, but if so there's a section of it that's missing because we don't see how Aziraphale's hand moves from Crowley's back (shot 3) to waving in the air. This isn't really a continuity error as presumably Aziraphale has been moving his hands around during shot 4 while the viewer was being treated to a close up of their faces, but it registers as a continuity error because the emotional vibe of shot 4 is Aziraphale starting to get into the kiss and lean in, while the hand off the back in shot 5 makes the viewer feel like he is pulling away and uncomfortable again.

In editing the sequence, I simply moved pieces around to try and tell a story that was more emotionally coherent, with Aziraphale starting shocked and uncomfortable, relaxing into the kiss slightly, and then stepping away. I chopped up the long sequence in shot 1 and used the relaxed part of it instead of the beginning of shot 5. I also switched shot 3 and 4 so that we see a wide cut where Aziraphale looks uncomfortable and his hand is visibly in the air, to a close up shot of their faces where he starts to get into it. Then we are in the home stretch with the tender hand on the back and then Aziraphale's face as he holds Crowley close to him (formerly the end of shot 1) before they break apart.

[kevinzhechair]

Meet the totally normal skeleton monster, not at all a timeline splicing Alien!!

Their name is Gleep, their pronouns are yes/all (he doesn't care about pronouns).

He used to be a commander of an alien arsenal who sneaked into alternative timelines to get code. Sometimes destroying or corrupting aus in the process.

After an injury, leaving it unable to do their job. The higher ups of the society decided not to kill them. Rather send her to a safe timelines the commanders had no need for (Underswap 113), as a type of vacation for his efforts.

They did not tell him that outright, so he still believes that the timeline is being used. Although that is not the case. The commanders enjoy his insane ramblings when he calls.

He's very up his own ass. Gleep is based off if you couldn't guess Zim from Invader Zim, but also Gnarpy from Regretavator. Why, because it's fun and I can.

[bpod-bpod]

Splice of Life

Splicing – the production of different forms of a protein from a gene's DNA instruction – in a sex-specific way occurs throughout the genome at the very earliest stages of embryo development

Read the published research paper here

Image from work by Mukulika Ray and colleagues

MCB department, Brown University, Providence, RI, USA

Image originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)

Published in eLife, March 2023

You can also follow BPoD on Instagram, Twitter and Facebook

[medicomunicare]

Indagando sull'autismo: le complesse interazioni fra dopamina, molecole di adesione ed energia cellulare

ASD e alterazioni della trasmissione cerebrale Il disturbo dello spettro autistico o ASD è un disturbo dello sviluppo che di solito si manifesta nella prima infanzia. Sebbene i risultati clinici varino notevolmente da caso a caso, l’autismo è caratterizzato sia da un interesse limitato per l’interazione sociale che da un comportamento ripetitivo. Ciò coincide con le interruzioni nella…

[fortunaestalta]

[honorarysimp]

Uncanny Distortion

Summary: Woodsboro, California is a small town that’s never known peace. For decades, people have gone missing without a trace. As the disappearances amp up, a Private Investigator is hired to crack the case. Will urban legends overshadow logic, or will the unexplainable lead to more questions?

Pairing: Tara Carpenter x Fem!Reader

Tags/Warnings: mature language, mentions of unsettling and disturbing events, childhood trauma, substance abuse, violence, angst, character deaths, slowburn; suggestive themes(18+)

AN: My take on an alternate reality of the Scream Universe, if it were lovecraftian horror spliced with uncanny folklore. Inspired by the feeling I got listening to this.

~Masterlist~

[kaidanworkshop]

Workshop Progress: Spring Update

Happy Pride Month Workshop Spectators! It's hot out there, so make sure you're staying safe and hydrated while celebrating in your respective communities. A lot has been going on behind the scenes since our last update; we've been busy tying up loose ends across the board as we finalize Kaidan Revoiced: Community Expansion, which is frankly, very tedious - lots and lots of spreadsheets. Nonetheless, all the miscellaneous minutiae we're ironing out is to ensure our upcoming surprise to the community, [REDACTED], runs smoothly. We've also got one final poll for you before our official release, then it's back to the grindstone for the Workshop staff as we focus on splicing and processing the last lines for implementation. As per our last poll, the community voted to have us postpone the mod release until it is in a finalized state.

Afterwards, we spent most of April going over the script one final time to catch any extra lines we may have missed for our commission covering the last of the original Kaidan 2 script. We also held a few live sessions on our Discord going over the few bits of Workshop Original content that will be going into this first version: the alternative platonic Autumnwatch route, the Nickname & Pronoun system, and the rewritten Pieces of the Past quest. All in all, this new content amounts to just over 100 extra lines; this version of the mod will be preserved indefinitely on our mod page in the future. As we move forward with creating and implementing more Workshop Original scripts post launch, users will still be able to download and use this version if they prefer. Finally, throughout the end of April and all of May, we've been running a closed beta for KR:CE with the help of volunteers from other Skyrim modding teams. We've been able to track down a few more bugs, confirmed that our new quest fail safes work (woo!), and discovered a handful of mods that were incompatible with KR:CE, requiring us to move a few things around.

During our closed beta testing, we did get a recurrent piece of feedback that we'd like to discuss with the community at large. Similar to the audio distortion Kaidan has in Kaidan 2, the voice lines for the Dremora in Kaidan's personal quest, as well as Myriah, the priestess in the forest wedding, are very badly distorted. One of the key reasons the Workshop was formed was to address the audio distortion that existed in the Kaidan 2 mod, so it feels incomplete to not address this issue. We'd like to hire two new voice actors to rerecord these lines for implementation, the cost of which would be approximately $40 - $60 USD total (due to how few lines we need), taken from our current budget surplus. As always, we turn to the community regarding any decisions relating to how we allocate your donations; you can vote on this proposition here through June 14th. As always, we thank you for your interest, your patience, and your support! We can't wait to announce [REDACTED], and the official release date of KR:CE to the community.

[gallifreyanhotfive]

Random Doctor Who Facts You Might Not Know, Part 13: Gallifrey at War Part 2

TW: more gruesome Time War shit, lots of death, disturbing stuff

Both the Daleks and the Time Lords investigated genetic manipulation to create new creatures to use during the Last Great Time War. This includes the highly psychic Time Lord called Quarren Maguire, who was altered to have reality changing abilities. He used these abilities to erase the evidence that he existed and then Chameleon Arched himself to pass as human, unaware of his nature as a Time Lord.

Marie is a sentient humanoid Type 103 TARDIS. The Time Lords kept her in a box for more than a year screaming and thrashing. Eventually, the High Council allowed her to mate with a Type 105 TARDIS, and she bore a baby TARDIS. After the baby was born, the Time Lords took them away from Marie immediately.

The Last Wave was a generation of soldiers in the War in Heaven. The officers in this group were older individuals who forced regeneration until their skin was coated in this black organic blastproofing.

Dalek technology possessed regeneration inhibitors.

The Time Lords would often splice different species together in the Time Vortex to create increasingly impossible and mindless beings.

Leela was fitted with a compliance collar during the Last Great Time War to force her obedience.

The Fifth Doctor became involved in the Last Great Time War by accident after crashing his TARDIS into a Dalek time machine.

During the War in Heaven, the Nine Gallifreys project concerned cryptoforming planets into Gallifreyan cloneworlds. Some envisioned every planet in the universe eventually being transformed. These clone Gallifreys would eventually be used as ballistic projectiles as the war escalated among other things.

In the Last Great Time War, mutated Time Lords dubbed "Interstitials" were living in the Death Zone. They were the results of experiments by Rassilon to create a possibility engine. This involved retro-evolving the timeline of these Time Lords to connect to the time vortex and enter a loop of iterative regeneration. This meant they had no constant appearance and were instead in flux between different bodies. Rassilon and Borusa eventually managed to build their engine using them.

In the beginning of the Last Great Time War, the War Council built munitions factories underneath Gallifrey's surface. They employed children.

It was said that a soldier in the Last Great Time War could die a thousand times in a single day only to learn that they had never been born at all the day after.

The Cold was a weapon created by the Time Lords during the War in Heaven. When activated, it breaks through the time-space continuum, so everything nearby gets sucked into an alternate universe and destroyed. If introduced to an inactive Cold, a subject could be held in stasis for centuries, like Fitz Kreiner was.

During his time working for the Faction, Kreiner spent centuries killing Time Lords, including the Rani and the Master although these may have been clones. He collected their severed heads.

Saturnyne was a water world until it was hit by a shock wave of temporal disruption from the Last Great Time War. This caused the planet to change to one where the inhabitants evolved with no natural laws of evolution, becoming odd beings that could move from the deepest depths of the water to land. The Saturnyne matriarch Rosanna Calvierri would later meet the Eleventh Doctor, Amy, and Rory in Venice.

The Time Lords began to seriously alter their biology during the War in Heaven, often becoming what the Eighth Doctor termed to be "monsters."

The biodata virus altered its victims biodata to become members of the Faction Paradox. It infected the Third Doctor while he was regenerating on Dust after being shot, thus dramatically altering the timeline. Now infected, the Doctor would be corrupted into a member of the Faction by the end of his Eighth self.

River Song used a therapy bot to erase the Eleventh Doctor’s memory of counting how many children were on Gallifrey at the end of the Last Great Time War. This same therapy bot would one day belong to Cass Fermazzi, but instead of taking her memories, it just spewed out the Doctor’s. This had a great effect on her childhood and inspired her to fight for what is right. Discovering this confirmed the Thirteenth Doctor’s belief that they caused her death.

Part 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28