#Skin Cancer Diagnostics

[paullui2002]

Skin Cancer Diagnostics Market Share, Sales Channels and Overview Till 2028

Skin Biopsy Segment by Screening Type to Lead Skin Cancer Diagnostics Market during 2021–2028. According to our latest study on "Skin Cancer Diagnostics Market Forecast to 2028 – COVID-19 Impact and Global Analysis – by Type and Screening Type " the market size is projected to reach US$ 3,730.12 million by 2028 from US$ 2,309.60 million in 2021; it is estimated to grow at a CAGR of 7.1% from 2021 to 2028. The report highlights the key factors and trends impacting the skin cancer diagnostics market and focuses on prominent players and their game-changing strategies.

The increasing incidence of skin cancer and launch of new products drive the market growth. However, the tedious and barriers to early skin cancer diagnostics in low and middle-income countries hampers the market growth. Skin biopsy segment held the largest market share for skin cancer diagnostics market. Skin biopsy is diagnosis procedure that require a small sample of the skin.

Get Sample PDF Here @ https://www.theinsightpartners.com/sample/TIPRE00018116/

During the skin biopsy procedure, doctor cuts and removes a small part from skin for the test. Skin biopsy is performed to determine skin cancer, infection or other skin conditions. The skin samples are collected by different types of biopsies such as punch, excisional, incisional, and shave biopsy.

These types of biopsies are performed for melanoma skin cancer. And in cases of non-melanoma, biopsy is often the only test done to diagnose the stage or the extension of the cancer as non-melanoma skin cancer rarely spreads. Similar, to other mean of screening biopsy is equally important to diagnose a skin cancer type. The increasing awareness and incidences of skin cancer are likely to drive the market during the forecast period.

Dermatoscopy held the second largest market share for the skin cancer diagnostics market. Dermatoscopy is the primary test for skin cancer. It increases the sensitivity for detecting skin cancer, decreases the number of benign lesions biopsied for each malignant diagnosis, and enables the diagnosis of thinner melanomas compared with naked eye examination. In addition, dermatoscopy is cost effective, which is leading to decreased number of excised benign lesions. Dermatoscope are available in several different lightweight models, and battery-operated hand-held devices. Furthermore, the advancements in the technology are enabling players to launch upgraded versions of their existing dermatoscopes and new dermatoscopes in the market. Thus, the technical development is another leading factor for increasing demand for dermatoscpes, which is vitally contributing to the growth of market.

SkylineDx BV; AMLo Biosciences Limited; NeraCare GmbH; DermLite LLC; bioMerieux SA; Veriskin Inc.; Castle Biosciences, Inc.; DermTech Inc.; HOFFMANN-LA ROCHE LTD., and Michelson Diagnostics Ltd are among the leading companies operating in the global skin cancer diagnostics market.

Read Complete Report Here @ https://www.theinsightpartners.com/reports/skin-cancer-diagnostics-market

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[dead-in-the-pool]

Dude don't you know someone who said to sniff for skin cancer?

first of all my friend said he could TASTE cancer which is a different thing entirely but 1 skin cancer is seldom found in a place you can lick and 2 i think he's full of shit anyway

[innonurse]

AI enhances the accuracy of skin cancer diagnosis in a Stanford Medicine-led study

- By InnoNurse Staff -

A study conducted by the Stanford Center for Digital Health found that deep learning-powered artificial intelligence algorithms increase skin cancer diagnostic accuracy for doctors, nurse practitioners, and medical students.

Read more at Stanford Medicine

[lastoneout]

I s2g these like...third party? clinics that doctors send you to for MRIs and x-rays and stuff like that are run by absolute clowns.

I get an order to go an ultrasound of a weird spot on my skin that's probably just torn soft tissue just to make sure nothing's going wrong and I call them and they schedule me for the 6th of June, but tell me that the order I got from the urgent care place needs to be from the clinic supervisor, not the NP who saw me, so I need to go get that fixed, and also to tell them that the NP isn't in their system so they need to fax them documents to update her info. Okay, I'm not an employee there so idk how that last bit is my problem, but whatever.

In between this call and me having time to go to the urgent care clinic I get a call telling me actually they don't have anyone who can do the ultrasound on the 6th so I need to come in on the 30th. Great, whatever. This has happened before, sometimes DAY OF with these types of places so while it's annoying, I'll live.

There's less time before my appointment now so same day I go down to the urgent care place and explain all that and the desk lady seems to have no clue what the hell I'm talking about and just gives me a copy of the order and says if the ultrasound place needs updated stuff they have to request it, which is the opposite of what I was told but whatever!

So I call the ultrasound place and explain and the guy is like "uh no you should be fine with the order" and then to be sure he checks the info and is like "oh this doctor's info is out of date" and then he has me stay on the line and give him the urgent care clinic's phone number and address and again! I am not an employee there!! Why is this my job!! But whatever, he says I'm good, so I'm good.

Today, the 29th, I get another call from the ultrasound place informing me that actually, they don't even perform this procedure in Tucson AT ALL and if I want to get it done I either have to GO TO PHOENIX or get my order sent to a different clinic that does these. I do not understand why this information was not given to me immediately the first time I called, and now I have to go down to the urgent care clinic AGAIN and beg them to update the order, and idk if they'll even be able to do that bcs this is an urgent care clinic not my PCP, but whatever guess I get to go beg! And continue to worry about the soft-tissue tear bcs it isn't going away on it's own and idk if there's something legit wrong with me or not.

I fucking HATE these little third party clinics so much they are always canceling the day before or telling me actually I need to go to another location bcs they can't do it at this one or claiming they called me to tell me the date got moved when I didn't get a phone call AT ALL. Why are they so poorly managed. Why does no one know who works at what location or what location does what procedure. Y'all are doing serious diagnostic tests to confirm if people have like cancer or some shit, I've had procedures done at them twice to make sure I don't have cancer, but they still play SO fast and loose with people's time and yank us around.

I genuinely miss the days when they would just send you to the hospital to have this stuff done, they aren't great but at least when they say they can do something they can actually do it. Jesus christ. I'm so tired.

[all-pacas]

DIAGNOSTIC OLYMPICS, SEASON 2, EPISODES 10-18

S1: part one, part two, part three

S2: part one

Hi! I was curious about who on House (besides House) gets the most diagnoses right. Other folks have already run a tally (it's Chase), but I was curious how other factors would influence the tally — whose ideas get run with, who manages treatment, who screws up… So I thought I'd keep score.

1 point for getting the answer. This is almost always going to be House.

.5 points for Valuable Contribution — stuff that isn't the final answer, but either is thought to be the final answer or is valuable to the solving of the case. Stuff like "noticing something on the MRI" doesn't count; things like "figuring out how to treat" does.

-.5 to -1 for Mistakes — stuff that delays or prevents diagnoses, injuring or killing patients, etc.

FAILURE TO COMMUNICATE DIAGNOSES: Brain Malaria

+1 HOUSE: Honestly, should get more than one point: he solves the case by phone with no information. That’s more because this is a weird and pretty weak episode, though. +.5 CHASE: Although he was wrong in the end, he got an early win realizing the patient was on drugs, and suggesting a lumbar puncture that only later (on House’s order) was done. That’s two good ideas in a episode with very few ideas at all. +.5 CAMERON: Finally, finally realizes the patient only talks when his wife isn’t around. +0 FOREMAN: Comes up with basically no ideas, and continues to struggle with leadership. Chase and Cameron actively rebel and refuse his orders, and he doesn’t do much to prove himself. +0 TEAM: The Fellows do a pretty terrible job all episode, missing the obvious and running all over the place. But the episode also really isn’t about the case, so whatever. Honestly, wanted to give out no merits this episode at all.

NEED TO KNOW DIAGNOSES: Just so many things. But mostly a tumor.

+1 HOUSE: Another episode where the team is mostly running around chasing symptoms, House figures out the last piece of the puzzle (that the patient was on birth control) at the last second. +5 CUDDY: Her exasperated Stacy impression is, actually, very good.

DISTRACTIONS DIAGNOSES: Anti-depressant poisoning

+1 HOUSE: A not-so complicated case where the main problem was just testing the kid and getting information. House stumbled onto the correct solution, almost killed the patient anyway, and then figured it out for real. +.5 FOREMAN: Now that he’s no longer in charge, he’s suddenly doing much better, suggesting smart ideas and tending the patient. His merit is specifically for doing a very dangerous lumbar puncture successfully.

SKIN DEEP DIAGNOSES: Testicular cancer

+1 HOUSE: Cracks the case, despite the Heightened Pain and all his sleazy comments. As ever, we don’t judge on words, only actions. +1 CUDDY: Not only refuses to misgender the patient and calls House out on it, she manages to completely pull one over on him with her morphine trick. Love to see it. +0 FOREMAN: He makes a big to-do about House rushing things and being wrong due to his pain and haste, but every call House makes this episode is completely right, so probably would have been better saved for another episode. -.5 CAMERON: Early in the episode, she and Chase are told to do an LP and an MRI. The patient has a twitch, so Cameron decides not to bother with the MRI at all. Oops! Now, later in the episode Wilson also did an MRI and didn’t find anything, but at the end of the episode, House did find the cancer during a third scan. In between, the patient got a brain biopsy. Giving Cameron the demerit because if she had done the MRI in the first place, it might have been found sooner, sparing the danger of a brain biopsy.

SEX KILLS DIAGNOSES: Cheese bacteria; STDs

+1 HOUSE: Figures out patient #1 is Cheese Sick pretty quickly — literally, only about fifteen minutes into the episode. +1 TEAM: Most of the episode is spent keeping patient 1 alive and getting patient 2 healthy enough to donate her heart. She turns out to have an STD, but no one person really “solves” the case. +5 MR. NEUBURGER: The episode is intentionally full of very sympathetic characters, but I liked him. I loved how he sort of inserted himself into the case and kept following House around, I liked House being more sympathetic than usual and letting him insert himself into all the DDX’s, it’s just a weirdly cozy episode.

CLUELESS DIAGNOSES: Gold poisoning

+1 HOUSE: Lands on heavy metal toxicity early, but they can’t figure out what metal for much of the episode. He then has a second eureka moment and realizes gold. +.5 FOREMAN: Becomes convinced it’s lupus. It isn’t, but when House continues to insist on heavy metal toxicity despite test results, Foreman not only stands up to him but wins, proceeding with the lupus treatment because House can’t come up with proof that isn’t “because I say so.” Foreman was wrong, but this might be the first time any of the fellows successfully stand up to House like this and get their way.

SAFE DIAGNOSES: Tick bite

+1 FOREMAN: When the patient’s heart starts failing, House wants to come up with a fancy reason the allergy would call it, but has no ideas. Foreman, for the second episode in a row, pushes back and gets his way: to investigate the heart failure rather than start guessing. He then takes charge of the team and whiteboard as House sits quietly, and more or less runs the rest of the case. House even admits he’s right! +1 WILSON: Able to come up with an excuse to get the patient in an elevator with House on the fly. It’s always great to see his lying skills in action. +.5 HOUSE: Realizes the tick bite after he finally talks to the patient, and then is able to find it through, uh, invasive means. +.5 CAMERON: First one to guess tick paralysis, although it’s quickly shot down because at the time they’re no longer thinking allergy. +.5 CHASE: Suggests botulism, which is a long shot but becomes House’s running theory. Also figures out that the patient’s boyfriend has been sneaking in to see her, and is the one to realize they never actually talked to her. The second they do, it clears everything up. No big revelations from Chase, but three little ones makes half a merit fair.

ALL IN DIAGNOSES: Erdheim-Chester

+1 HOUSE: Goes in thinking it’s Erdheim-Chester, and it is; the test just gives a false negative. The rest of the episode is spent throwing things at the wall until he finally is able to prove it. +.5 CHASE: Despite starting the episode whining and skeptical, he gets points for his first guess once he is committed: House compliments it and calls it not just good but exactly what he did with Esther. It’s wrong, but points for a literal House-level theory.

SLEEPING DOGS LIE DIAGNOSES: Bubonic Plague

+1 HOUSE: Everyone is running around with no good guesses, and get distracted by the liver failure, but House pulls it out in the end. +0 CAMERON: While she complains and gets bogged down by the ethics of toxic lesbianism, and is certainly not winning points for bedside manner, she doesn’t let her distaste screw her up. Character development! +0 CAMERON AND FOREMAN: Foreman didn’t actually “steal” Cameron’s article, but he was a huge asshole about it all the same. They both lose points somewhere, but not here for this. +500 TOXIC LESBIANS: I think about these women every single day. This episode is simply incredible and one of the writers clearly has had some Experiences and I salute her for them

RUNNING TALLY:

HOUSE: 32 (+8.5) TEAM: 5.5 (+1) FOREMAN: 3 (+2) CHASE: 3.5 (+1.5) CAMERON: 3.5 (+.5)

Foreman is finally catching up, ironically only after he lost his temporary leadership role. I guess you could say that the experience gave him confidence — his points are mainly for standing up to House and taking charge.

[darkmaga-retard]

Don't miss this episode, especially if you have breast cancer! This may save your life. And SHARE IT with everyone! This information is highly censored because "they" don't want you to know the truth.

The Truth About Cancer

Aug 30, 2024

TONIGHT We are airing FREE to the world our GROUNDBREAKING Episode 2 “Cancer Facts and Fictions, Breast Cancer, Hormones, Skin Cancer & Essential Oils”

So many of you tuned in and watched Episode 1 right here so we wanted to give you a portion of Episode 2 here tonight. This episode, we pull back the curtain on the lies you’ve been told.

Here’s what you’ll uncover…

✅The real deal on how cancer spreads—prepare to be shocked.

✅Breast cancer diagnostics: Which ones actually work, and how mammograms could be doing more harm than good.

✅The overdiagnosis epidemic: Why false positives are scaring women into unnecessary treatments.

✅Hormones and cancer: Big Pharma’s dirty little secret they hope you never figure out.

✅Essential oils that prevent and heal cancer—and why doctors who use them are under attack.

✅Natural remedy from Australia that reversed 70,000 skin cancers … and got the doctor thrown in jail!

✅Erin Elizabeth spills the truth about Breast Cancer Awareness Month and “Think Pink”—how a multinational giant hijacked the idea from a sweet old lady.

✅Mammograms vs. Thermograms: Why the Medical Industry is obsessed with pushing mammograms, despite the fact that thermograms are safer and better.

✅Dr. Veronique’s “7 Essentials”: Your guide to preventing and treating breast cancer—why isn’t everyone talking about this?

✅And so much more...

Don’t let Big Pharma keep you in the dark—watch this episode and arm yourself with the truth.

Reserve Your Free Seat to watch The Truth About Cancer’s “A Global Quest” here: www.TheTruthAboutCancer.tv/Substack

[careforcritters]

Vet Med in Warrior Cats: Oncology (Cancer)

Disclaimer

Pebblefur, a Windclan deputy who appears in Code of the Clans, was stated to have died from a “strange, agonizing lump in his belly”. Shellheart, a former Riverclan deputy and father to Crookedstar, was also stated to have a similar painful lump in his abdomen, which caused him to retire and is suspected to be the cause of his death. The medicine cat in Crookedstar’s Promise, Brambleberry, stated that she had seen this condition before and that cats who suffer from it never get better. Ravenpaw has this same condition in Ravenpaw’s Farewell, which leads to his death.

These cats are likely suffering from a form of gastrointestinal cancer, and the most common presentation of GI cancer in cats is small intestinal lymphoma. Signs of intestinal cancer include vomiting, inappetence, diarrhea, and weight loss. While vomiting and diarrhea were not mentioned in the books, Shellheart and Ravenpaw both appear to be suffering from pain and gradual weight loss. Intestinal tumors can also lead to perforation, which would cause death fairly rapidly. Most lymphomas are small cell, which is a low grade lymphoma, and has a slow onset and progression of clinical signs. This is consistent with the three cats in warriors who were seen to have cancer, as Shellheart and Ravenpaw both knew that they were dying as their pain slowly got worse. 

In a veterinary clinic or hospital, small intestinal lymphoma would be diagnosed with a combination of histopathology, immunohistochemistry, and PCR for Antigen Receptor Rearrangement. It would be treated with a combination of chlorambucil (a chemotherapy drug) and prednisolone (an anti-inflammatory). Expected survival time is about two years with treatment. Medicine cats would have no way to know what the cause of the symptoms was, and would have no treatments for it even if they did. The best that they can do is to provide supportive care and try and ease their pain until death.

In the novella Cloudstar’s Journey, Petalfall is described as having periodic seizures and progressive weakness after suffering from an illness. As the prey grows scarce in Skyclan, she eats less and becomes weaker and has more frequent seizures until she passes. It is possible that Petalfall could have had a tumor in her brain since they can cause seizures, but this is never confirmed. Since the seizures were noted to have started after Petalfall fell ill, she is more likely to be suffering from meningitis or encephalitis after the infection spread to her nervous system. This topic will be covered in the neurology section. 

Other common cancers in cats that are not seen in warriors include squamous cell carcinoma of the mouth, skin, and nose, fibrosarcomas in skeletal muscles, and mammary tumors in intact females. All of these tumors could be visible in later stages without imaging or surgery, but none of them appear to be mentioned in the series. As stated before, medicine cats don’t have diagnostic or treatment options for any type of cancer. One explanation for this is that the warriors’ lifespans are shorter than most domestic cats and that many characters die of other causes before reaching an advanced age. However, since they live outside, have near constant sun exposure, and none of them are spayed or neutered, I would expect a much higher rate of squamous cell carcinoma (especially in pink skinned cats) and mammary gland tumors.

sources:

Gieger T. Alimentary lymphoma in cats and dogs. Vet Clin North Am Small Anim Pract. 2011 Mar;41(2):419-32. doi: 10.1016/j.cvsm.2011.02.001. PMID: 21486644

Withrow and MacEwen’s Small Animal Clinical Oncology

[bugcowboyart]

The Sanktis Project // Repentant Trickster Veledon

The god of Health and Healers, protector from disease began their godhood as a minor trickster. When their sole mortal servant begged the god l save her wife, Vel set out to steal part of the long silence’s power— the piece that effects the body.

Successful, the trickster went on to give healing to many, and devoted themself to their new position as god of healers (though sometimes they play tricks like sinus inversus or the like).

The Continent for the most part relies on a modified Miasma theory of disease— the idea that miasmas or “bad air” are the cause of contagious disease.

Clerics of Veledon spend the majority of their time organizing city infirmaries, lazarettos, plague quarters, and in rare times of war are voluntarily attached to army units.

Not all healers take holy orders, instead choosing to open private practices or specialty shops.

Magical healers in the order have a wide array of healing abilities—seers able to look inside bodies, burn out infection, detect cancer, or heal broken skin and bone. Surgeries are done with physical tools, magic-enhanced anesthetic, and healers to close. Non-magical healers serve in the order as surgeons, potion makers, nurses and in diagnostic capacities even without magical abilities. Potions and poultices are often made en masse by non-magic means and then enhanced or activated as needed by the magical clerics.

While germ theory hasn’t been figured out yet, magic and the knowledge of the body it reveals has been able to prolong lives and raise quality of living.

[alwaysdial]

Dermatologist

Dermatology 

Dermatology is the medical discipline that is concerned with diagnosis and treatment and treatment diseases of the skin, hair and nails

in both children and adults. Specialists in dermatology are called Dermatologist.

Dermatology involves but is not limited to study, research, and diagnosis of normal and disorders, diseases, cancer, cosmetic and ageing conditions of the skin, fat, hair, nails and oral and genital membranes, and the management of these by different investigations and therapies, including but not limited, topical and systemic medications, dermatologic surgery and dermatologic cosmetic surgery, immunotherapy, phototherapy, laser therapy, radiotherapy and photodynamic therapy.” 

 Medical professionals

 Best cardiology Clinic in Gaya Dermatologists treat patients with common conditions, like acne or wrinkles, but they also can treat skin cancer and other chronic or painful skin diseases. Dermatologist might perform tasks like examining patients on how to take care of their skin.

 Education and Training

 It includes an undergraduate pre medical degree, general medical training, internship and dermatology specialization training. You must have high NEET UG score. To complete four years of medical school, you must enroll in an MBBS program. The next stage is obtaining a one-year dermatology internship or another field. Following an internship, three years of residency or continuing training in dermatology is also required.

 Skin Condition

Acne

Hives

Warts

Fungal nail infection

Cold core

Candidiasis

Athlete’s foot

 Cosmetic Dermatology

 Cosmetic dermatology is a specialized field of dermatology that focuses on procedures that improve appearance of the skin, particularly on the face and neck. It is also known as aesthetic dermatology or aesthetic medicine.

Skin Cancer Screening

 A visual self-exam by the patient and a clinical examination by the health care provider may be used to screen for skin cancer. Best oncology Clinic in Gaya During a skin exam a doctor or nurse checks the skin for moles, birthmarks, or other pigmented areas that look abnormal in color, size, shape, or texture.

 Hair and Nail Disorders

 Most common Hair problems

 If you are experiencing issues with your hair or nails, it is important to see a dermatologist. At water’s Edge Dermatology, our providers have years of experience treating hair and nail problems.

��Most common Nail Problems

 Our Nails are important. They protect and support the tissues in our fingers and toes. And they allow us to scratch an itch.

But nail problems are common. As we age, nails thicken and become more susceptible to issues such a fungal and bacterial infections. Often these issues are nothing to worry about, but sometimes a nail problem can signal and underlying disease.

Allergies and Skin Sensitivities

 The most common allergic skin conditions are atopic

Dermatitis (eczema), allergic contact dermatitis (rash where allergen touched the skin), urticarial (hives), and angioedema (swelling). Hives and swelling often happen together, and may suggest a serious and life threating allergic reaction. 

 Preventive Care

 Some of the most important preventive measures are, avoiding too much sunlight, applying sunscreen before going out, covering up skin in the sun, and avoiding tanning booths. Best neurology Clinic in Gaya

 Research and Advancements

 Research advancements in dermatology have brought about transformative changes in the diagnosis, treatment, and management of various skin conditions. Innovation in diagnostic tools, therapeutic modalities, and digital health technologies have significantly improved patient care.

Patient Education

 By Definition, patient education is the process of influencing patient behavior and producing the changes in knowledge, attitudes and skills necessary to maintain or improve health through the provision of information and the teaching of patients.

Get in Touch

Contact :- 9117999911

Website:- https://www.alwaysdial.com/

Mail Id :- Alwaysdial.team@gmail.com

[jcmarchi]

Noninvasive Technique Reveals How Cells’ Gene Expression Changes Over Time - Technology Org

New Post has been published on https://thedigitalinsider.com/noninvasive-technique-reveals-how-cells-gene-expression-changes-over-time-technology-org/

Noninvasive Technique Reveals How Cells’ Gene Expression Changes Over Time - Technology Org

MIT researchers can now track a cell’s RNA expression to investigate long-term processes like cancer progression or embryonic development.

DNA – artistic impression. Image credit: Image by kjpargeter on Freepik

Sequencing all of the RNA in a cell can reveal a great deal of information about its function and what it is doing at a given time. However, the sequencing process destroys the cell, making it difficult to study ongoing changes in gene expression.

An alternative approach developed at MIT could enable researchers to track such changes over extended periods of time. The new method, which is based on a noninvasive imaging technique known as Raman spectroscopy, doesn’t harm cells and can be performed repeatedly.

Using this technique, the researchers showed that they could monitor embryonic stem cells as they differentiated into several other cell types over several days. This technique could enable studies of long-term cellular processes such as cancer progression or embryonic development, and one day might be used for diagnostics for cancer and other diseases.

“With Raman imaging you can measure many more time points, which may be important for studying cancer biology, developmental biology, and a number of degenerative diseases,” says Peter So, a professor of biological and mechanical engineering at MIT, director of MIT’s Laser Biomedical Research Center, and one of the authors of the paper.

Koseki Kobayashi-Kirschvink, a postdoc at MIT and the Broad Institute of Harvard and MIT, is the lead author of the study, which appears today in Nature Biotechnology. The paper’s senior authors are Tommaso Biancalani, a former Broad Institute scientist; Jian Shu, an assistant professor at Harvard Medical School and an associate member of the Broad Institute; and Aviv Regev, executive vice president at Genentech Research and Early Development, who is on leave from faculty positions at the Broad Institute and MIT’s Department of Biology.

Imaging gene expression

Raman spectroscopy is a noninvasive technique that reveals the chemical composition of tissues or cells by shining near-infrared or visible light on them. MIT’s Laser Biomedical Research Center has been working on biomedical Raman spectroscopy since 1985, and recently, So and others in the center have developed Raman spectroscopy-based techniques that could be used to diagnose breast cancer or measure blood glucose.

However, Raman spectroscopy on its own is not sensitive enough to detect signals as small as changes in the levels of individual RNA molecules. To measure RNA levels, scientists typically use a technique called single-cell RNA sequencing, which can reveal the genes that are active within different types of cells in a tissue sample.

In this project, the MIT team sought to combine the advantages of single-cell RNA sequencing and Raman spectroscopy by training a computational model to translate Raman signals into RNA expression states.

“RNA sequencing gives you extremely detailed information, but it’s destructive. Raman is noninvasive, but it doesn’t tell you anything about RNA. So, the idea of this project was to use machine learning to combine the strength of both modalities, thereby allowing you to understand the dynamics of gene expression profiles at the single cell level over time,” Kobayashi-Kirschvink says.

To generate data to train their model, the researchers treated mouse fibroblast cells, a type of skin cell, with factors that reprogram the cells to become pluripotent stem cells. During this process, cells can also transition into several other cell types, including neural and epithelial cells.

Using Raman spectroscopy, the researchers imaged the cells at 36 time points over 18 days as they differentiated. After each image was taken, the researchers analyzed each cell using single molecule fluorescence in situ hybridization (smFISH), which can be used to visualize specific RNA molecules within a cell. In this case, they looked for RNA molecules encoding nine different genes whose expression patterns vary between cell types.

This smFISH data can then act as a link between Raman imaging data and single-cell RNA sequencing data. To make that link, the researchers first trained a deep-learning model to predict the expression of those nine genes based on the Raman images obtained from those cells.

Then, they used a computational program called Tangram, previously developed at the Broad Institute, to link the smFISH gene expression patterns with entire genome profiles that they had obtained by performing single-cell RNA sequencing on the sample cells.

The researchers then combined those two computational models into one that they call Raman2RNA, which can predict individual cells’ entire genomic profiles based on Raman images of the cells.

Tracking cell differentiation

The researchers tested their Raman2RNA algorithm by tracking mouse embryonic stem cells as they differentiated into different cell types. They took Raman images of the cells four times a day for three days, and used their computational model to predict the corresponding RNA expression profiles of each cell, which they confirmed by comparing it to RNA sequencing measurements.

Using this approach, the researchers were able to observe the transitions that occurred in individual cells as they differentiated from embryonic stem cells into more mature cell types. They also showed that they could track the genomic changes that occur as mouse fibroblasts are reprogrammed into induced pluripotent stem cells, over a two-week period.

“It’s a demonstration that optical imaging gives additional information that allows you to directly track the lineage of the cells and the evolution of their transcription,” So says.

The researchers now plan to use this technique to study other types of cell populations that change over time, such as aging cells and cancerous cells. They are now working with cells grown in a lab dish, but in the future, they hope this approach could be developed as a potential diagnostic for use in patients.

“One of the biggest advantages of Raman is that it’s a label-free method. It’s a long way off, but there is potential for the human translation, which could not be done using the existing invasive techniques for measuring genomic profiles,” says Jeon Woong Kang, an MIT research scientist who is also an author of the study.

Written by Anne Trafton

Source: Massachusetts Institute of Technology

You can offer your link to a page which is relevant to the topic of this post.

[hannahlovesluca]

Hi I was wondering if you could make a matchup with nijisanji if possible?

Both sfw and nsfw

Gender: gender-fluid (biologically female)

Pronom: she/him

Sexuality: pansexuel

Appearance: 5’7 (170cm), dirty blonde hair (sometimes dyed) medium length, tan skin with a bunch of beauty spot and two birth mark (one on my left leg close to my inner thigh and the other one pretty big on my arm), I am pretty slim, I have glasses, brown/black eyes and dimple

Personality: im very shy and quiet but when I’m with people I’m comfortable with I am a bit crazy, sarcastic and a bit of a prankster but overall I’m caring, kind, smart, a hopeless romantic and patient

Zodiac sign: cancer

MBTI type: INFP

Likes: I love video games, drawing, music, reading, watching anime, plushie, horror stories, writing poem, sleep and cooking

Dislike: Loud noise/heated arguments (they scare me), not be able to fight back, pushy people, entitled people/brat, banana, frog, worms

Hobbies: play video game (of any type), drawing, listening to music, reading, singing and watch anime

Secret: I have ADHD and dyslexia (possibly autistic but I don’t have a diagnostic for that one) and I’m very good with kid/baby and they all love me (to the point they follow me like little ducklings) and I do want kid in the far futur

Misc: my native language is French but I’m more comfortable with English

Sorry if it was long or if there’s any grammatical errors I hope you have a good day and I hope it would be my Oshi that I get.

Thank you very much!

i pair you with…

Elira Pendora!

hear me out…

• UGH I LOVE HER.

• niji girls will playfully argue w you about who loves her more

• pogmu and you have a full rivalry

• luca probably ends up having a dating show w/ elira as the bachelorette and its just u and pogmu fighting for ewiwa’s heart

• oh my god i love ewiwa

• non parasocial

• she’ll probably be in call w/ you while you stream Melatonin and will end up falling asleep<3

• will hit you w random ass deez nuts joke

• or will sheesh at you if you tell her a pickup line

• shes such a zoomer ong

• pls tease her ab being a zoomer 🙏🙏

• if you think shes a zoomer she will force you to meet her brother and endure that pain

• she loves words of affirmation!!! giving and receiving!!!

• pls pls pls reaffirm her shes so insecure omg

• when holding ur hand she’ll do the thumb stroke thingy <33

RUNNERS UP: Luca Kaneshiro, Pomu Rainpuff

[pagerunner-j]

for the record:

waiting for days for biopsy results (especially when you're actively in pain in the meantime and no one will give you anything until you get the results) is THE FUCKING WORST

just so you know

(To be clear, nobody suspects I have cancer; it's a(nother) diagnostic for thus far unidentified autoimmune problems. So no matter how bad I feel, this could be even worse! But IT STILL SUCKS and my skin has been an inflamed disaster for weeks and it really fucking hurts, and I haven't been able to sleep properly, either. So. Yeah. Not loving life right now.)