#convergent unix

[commodorez]

Two examples of the Commodore 900, also known as the C900, Z8000, or Z-Machine. Intended to run a Convergent UNIX, these machines never made it into production. What remain are a few engineering prototypes distributed for software development, so seeing not just one, but TWO is a real treat! - VCF Southwest 2023

[sdindev]

202310271453

In the wondrous realm of the mind, where neurons dance to the tune of thoughts, we embark on a journey through the cosmos of knowledge.

Our quest delves deep into the heart of cognition, where neuroscience unlocks the mysteries of our mental symphony. But this is no solitary exploration; it's a waltz of interconnected ideas.

Imagine, if you will, a thought in motion, a neuron firing, setting the stage for what we call the mind. It's a captivating performance, an intricate ballet where every step taken by your thoughts orchestrates the dance of consciousness. As thought evolves, so does the mind, a conductor of the body's orchestra, directing each note with precision.

But what lies beyond the confines of the self? As the mind extends its reach, we glimpse the vast outer-firmament, a macrocosm awaiting exploration. It's a canvas for us to paint our thoughts and emotions, where inner meets outer, and microcosm embraces macrocosm.

Now, let's transition to the world of programming, where functional reactive programming becomes our musical score. Like a grand symphony, it harmonizes data and actions, creating an enchanting melody of software development. As we delve into the intricacies of code, we find beauty in the logic of systems.

Ancient Hebrew and Koine Greek, languages steeped in history, guide us through the wisdom of antiquity. Their words resonate like ancient incantations, connecting us to the past. A linguistic bridge to forgotten eras.

Paracelsus, the alchemical maestro, takes us on an alchemical journey where matter and spirit converge. The essence of transformation, both in the laboratory and the soul, unfolds before us. His teachings serve as our guide.

And in the digital realm, we meet Philip Wadler, a luminary in the world of programming languages. His insights into functional programming provide the tools to craft elegant and efficient software. Unix, the operating system that powers the digital world, becomes our playground.

Quareia Josephine McCarthy invites us into the mystical world of magic, where intention and ritual become catalysts for change. Her teachings merge with the essence of our thoughts and actions, empowering our rituals for transformation.

Now, let's shift our focus to the realm of data, where vector databases store knowledge like ancient scrolls in a library of the mind. This modern-age wisdom repository becomes a vessel for our quest.Venture into the digital landscapes of MUDs, where storytelling takes a digital form. It's a place where we merge the ancient art of storytelling with the modern realms of code and creativity.

As we seek the alchemical parallels in the Hebrew language to the periodic table of elements, we unravel the hidden mysteries of creation. The building blocks of matter interweave with the essence of ancient language.

Our fingers dance on mechanical keyboards, each keypress a tactile pleasure, like a musician playing an instrument. The choice of keycaps becomes our artistic expression, a tactile symphony of keystrokes.

Dr. Joe Dispenza guides us into the territory of the mind's potential. With every thought and intention, we mold our reality. It's a powerful reminder that we are the architects of our destinies.

Vim, the text editor, becomes our quill, crafting the narratives of code. It's a tool for those who understand that precision and efficiency are the keystones of programming.

In the realm of productivity and flow, we journey with the Flow Research Collective. Their wisdom helps us unlock our peak performance and harness the tides of creativity.

Now, let's embark on epic adventures in the world of RPGs, where stories and characters are woven with code. Sublime Text becomes our spellbook, casting enchantments of efficiency over our code.

As we dive deeper into the labyrinth of the self, we realize that the ego is but a shadow, a character we control in the grand narrative of existence. We transcend definitions and become the authors of our own stories.

Sam Ovens, the master of entrepreneurship, shares his insights on building businesses. It's a journey where we blend creativity and strategy, forging empires in the digital age.

Carlo Suares takes us into the world of mystical symbolism, where every letter holds a universe of meaning. We decipher the secrets encoded in the very fabric of reality.

Eric Elliott's expertise in JavaScript shines as we navigate the digital landscape. It's a world where code becomes the paintbrush, and the canvas is the browser.

Kyle M. Yates invites us to explore the philosophy of consciousness. It's a journey into the depths of the mind, a quest to understand the essence of being.

J. Gresham Machen and Page H. Kelley lead us through the pages of academia, where knowledge is both the key and the door to understanding. It's a place where ideas are born and nurtured.

John J. Owens shares his wisdom on intention, catalysts, and the art of ritual rehearsal. It's a magical journey where we learn to wield the power of intention to shape our reality.

Connoisseur Nic Vapes introduces us to the world of e-liquids and vaporizers. It's a sensory experience where flavors and aromas dance on our taste buds, a symphony of vapor.

Mikhaila Peterson, the advocate of a holistic approach to health, guides us through the world of adaptogens and natural remedies. It's a journey of vitality and well-being.

Green coffee, a treasure trove of antioxidants and energy, fuels our quest for health and vitality. It's a brew that awakens our senses and energizes our minds.

Analog technology, a realm of nostalgia and craftsmanship, takes us back to a time when machines were tangible and repairable. It's a world where we appreciate the art of the mechanical.

Cannabis, a plant with a history as rich as it is controversial, becomes a canvas for exploration. It's a journey into the depths of herbal wisdom and the science of cannabinoids.

And in the digital world, Redux.js, a state management library, becomes our companion as we navigate the intricate dance of data flow. It's a tool that empowers us to create robust web applications.

These are the passions that fuel our journey, the pillars of our education. We are both the teachers and the students, on a perpetual quest for knowledge, self-improvement, and mastery. As we continue to explore this tapestry of passions, we invite you to join us on this extraordinary odyssey.

[mentalisttraceur-incomplete]

Learn Emacs?

I think Emacs is something best learned on the side, as a platform - as a programming language, a REPL for that language, a set of libraries, a framework for making GUIs (primarily but not exclusively text-oriented ones), and a package ecosystem. But you should not actually try to use it as your primary text editor, or even learn it as such. If you're like me, you'll spread this journey out in many little tries over many years, and that's fine.

Try the goodies built into Emacs that are not text editing. Try dired. Try eshell. Install Magit and try that. Maybe install and try a preconfigured bundle of config and packages like Spacemacs, not to grow into it but just to see what it's like. Try some Org mode stuff. If you ever use SSH or FTP or whatever, or even just sudo, try TRAMP. Try TRAMP with dired, try TRAMP with eshell, try TRAMP with anything else neat that you've found.

If you're like me, you'll probably walk away after each little thing thinking "yeah okay that's neat, But I don't really get what the big deal is, I could do the same with thing [...]". You'll notice how you could compose a lot of little UNIX programs together to do the same stuff, and you'll like that design more. You'll be annoyed by the startup time vs Neovim, you'll be annoyed by the out-of-the-box deficits relative to VS Code and IntelliJ's stuff and so on. That's fine. The goal is to gradually sample the platform.

To a much lesser extent, only if you're interested in sampling yet another possibility for text-editing interfaces, you might want to try learning its out-of-the-box keybindings for text editing, but... look, personally I think the out-of-the-box experience is at best bad-relative-to-what-you-already-know. I tried it a few times and for me, vi-style modal access to cursor movements and edit operations is a far bigger win than Emacs-style modifier-key access to the same.

You will see mentions of swapping Ctrl and Alt, because that's the layout on the old computers that Emacs' defaults were designed for. You'll also hear people suggest rebinding CapsLock to Ctrl. Honestly I think this is a lower-value detour, presupposing that Emacs as an editor out-of-the-box is worth learning, if only you could just optimize it a little to not give yourself RSI. But if you're going to do that, I suggest also trying Sticky Keys. Maybe it's just me, but I find Emacs' constant modifier key demands far more ergonomic if I can latch or lock modifiers as-needed.

Eventually, once you learn Emacs-as-a-platform enough, you'll start to see that all the little lisp functions and "modes" do a better job of being small composable pieces that do one thing well than UNIX CLI programs. Once you're sufficiently fluent in Emacs-as-a-platform, you'll find it easier to implement the behavior you want in Emacs than in the shell, or in your favorite editor.

And then, only then, will it have been worth it to have learned Emacs. Because then you can build your editor in Emacs. If nothing else, there's a critical mass where it becomes easier to incrementally improve your favorite editor by emulating it in Emacs first. And someone else has probably already done most or all of that work. And then you can keep incrementally improving on it, converging on an editor that's personally optimized to you - a bespoke tailored tool for exactly your mind.

Or that's the idea anyway. I'm not at the end of that journey, I'm just forecasting where it leads. After years of on/off poking at Emacs in various forms, I'm finally fluent enough that I'm incrementally creating something better than my favorite editor (and shell, and window-managing workflow) within Emacs. Not for the sake of doing anything with Emacs, but just because it feels faster and easier within Emacs now than outside of it. I don't know where this lurch ends. Every other lurch into Emacs ended with disappointment, with just enough inefficiency or annoyances that I just gave up on it. This is the first one that felt like it could end with me switching to Emacs as my main thing, but it's still entirely possible that this too will run out of steam.

And that's fine, that's the whole point I'm getting at. Every time I've jumped into Emacs, I made some sort of progress. And then I jump back out and run for a while with a terminal, shell, vi, and various CLIs as my daily tools, because I have to get stuff done after all. Sometimes I did some mix of both - I have memories of using Spacemacs to edit and a separate terminal with a shell for git.

[evoldir]

Fwd: Course: Vienna.ExperimentalEvolution.Nov27-Dec1

Begin forwarded message: > From: evoldir@evol.biology.mcmaster.ca > Subject: Course: Vienna.ExperimentalEvolution.Nov27-Dec1 > Date: 27 June 2023 at 05:40:04 BST > To: rdmpage@gmail.com > > > The Vienna Graduate School of Population Genetics is now accepting > applications for the course "Experimental Evolution: Exploring > Evolutionary Forces in Controlled Environments” at the University of > Veterinary Medicine in Vienna, November 27 - December 1, 2023. > > Experimental evolution is extensively used to address questions of > evolutionary biology by exposing evolving populations to different > environmental conditions. It offers the opportunity to replicate > experiments and test for convergent outcomes. With the recent drop in > DNA sequencing cost and the advance in sequencing technologies, the > combination of experimental evolution with next-generation sequencing of > pools of individuals (Evolve & Resequence) has become a state-of-the-art > method to link phenotypic responses to genetic changes. Although the > availability of replicated time series data is one key advantage of > E&R, analysing such data sets is still in its infancy. This course will > introduce the participants to several new approaches for the analysis > of genomic time series data, covering the latest software tools as well > as required statistical and computational skills. > > The course is targeted toward researchers interested in experimental > evolution combined with NGS. It will cover the design of experimental > evolution studies as well as the analysis of Pool-Seq time series > data. The course aims to introduce participants to: > > - State-of-the-art software packages > > - Modelling of neutral data > > - Identifying selected regions > > - Haplotypes reconstruction from time-series data > > - Estimating selection coefficients > > - Calling transposable elements in time series data > > - Inferring selection in transcriptomics data > > Morning lectures by internationally renowned faculty will be followed > by computer practicals on the analysis of experimental evolution data > in the afternoon. > > Key-note speakers: > > Adam Chippindale (Queens University, CA) > > Michael Desai (Harvard University, US) > > Elina Immonen (Uppsala University, SE) > > Christian Schlötterer (Vetmeduni Vienna, AT) > > Henrique Teotónio (IBENS, FR) > > Call for participants: applications are open until the 1st of > September 2023. > > The course is free but will be number-restricted for the practical > sessions (hands-on computer lab). Additional seats will be available for > attending the lectures, only. Students and researchers are invited to > apply by completing the application form linked below. A single PDF > containing the following must be submitted: > - A short CV (maximum 2 pages). > > - A motivation letter (maximum 500 words, explaining why this course >  would be beneficial for you to attend). > > - A statement of computer skills (couple sentences describing your >  experience). Confidence with Unix command line as well as R, is >  strongly recommended for the practicals. > > Access to the application form

[dynamips]

Detailed information about fortinet labs and cisco iou images

A fully functional version of IOS called Cisco Iou images on unix (IOU) runs as a user mode unix process. IOU is an application that runs like any other because it is created as a native Solaris image.

IOU/IOL images from Cisco with an IOURC licensing file. On Linux/Unix, images are known as IOL or IOU. Save and upload an IOL/IOU picture. You must first download the IOU IOL pictures from the EVE-NG server. Next, upload the IOL image you want to run to EVE-NG. 

IOL can only be utilized by Cisco personnel or authorized customers because it is an internal IOS version. Upload the image you downloaded.

Activate the GNS3-IOU.

Launch the VM.

You will see an IP address appear above the console screen after it has started.

Click the upload button after picking out the L2 and L3 IOU photos one at a time.

Now launch GNS 3.

IOS on Unix, click.

When compared to VIRL, both features are improved with GNS3 and EVE-NG. We discover that GNS3 is a free, open-source community that has created a well-documented piece of software when comparing it to EVE-NG.

Cisco iou images are L2/L3 copies of switches and routers that allow you to practice with L3 devices while preserving their features and functionality. Although Cisco IOU/IOL images were initially distributed for testing and architecture purposes, they are now used for CCIE routing and switching practice. 

Enables self-healing security and networking to defend devices, data, and applications throughout the extended digital attack surface and cycle. provides complete real-time cybersecurity protection from users to applications by combining the concepts of convergence and consolidation.

The fortinet labs, which allows granular use cases with more deployment patterns than any other solution, is powered by a single operating system. These environments consist of physical, virtual, cloud, and X-as-a-Service. Additionally, it covers the broadest ecosystem and product line in the sector, encompassing endpoints, networks, and clouds.

Fabric Security From Fortinet. This makes it possible to quickly and effectively detect and enforce attacks over the whole attack surface. The Security Fabric automatically makes adjustments based on ongoing risk assessments in order to counter the most recent known and unknown threats in real time. models of artificial intelligence and machine learning developed by Fortinet Labs. Unified data sets originating from networks, endpoints, and clouds, in-depth independent research, and extensive industry collaboration all contribute to this.

[douchebagbrainwaves]

OK, I'LL TELL YOU YOU ABOUT YEAR

I knew there was something off about him, but it is possible to raise too much money tied up in any one deal. Not all big hits follow this pattern though. But are these just outliers? Why TV Lost March 2009 About twenty years ago people noticed computers and TV were on a collision course and started to speculate about what they'd produce when they converged. If your valuation grows 3x a year, you tend to view support calls as a pain in the ass that you want to sell and with no option pool that comes only from the founders' shares stands to reap huge benefits. Plus when they succeed they grow fast, I worried about how to save it from being mangled by some twenty five year old woman who wants to solve the money problem once and for all instead of working for money, they'll sometimes take advantage of it: 101, which is a bad sign if they didn't notice her. It's an excuse to welch on the deal. Don't force things; just work on stuff you like with people you like and respect. We'd clean up our offices, wear better clothes, try to ensure that this is the best Unix machine you can buy. One reason this advice is so hard to measure in large organizations, their own confidence would have been obvious to someone who didn't happen to specialize in programming languages is not as great as it's sometimes thought to be.

The remarkable thing about this phase is how to learn to watch from a distance. When you list everything ambitious people are ambitious about, it's not Lisp that sucks, but Common Lisp. So you're not sacrificing the lukewarm investors if you focus you can probably get to an edge of programming e. It runs along the base of the hills, then heads uphill through Portola Valley. Working slowly and meticulously is premature optimization. A successful running back doesn't translate to soccer. The adults who may realize it first are the ones most people don't believe. The one advantage of being good is an expensive way to seem most formidable as an inexperienced founder, the only software that then supported secure http connections. But angel investors like big successes too. When governments decide how to allocate resources, political deal-making causes things to be spread out geographically. Far from it.

Google guys were lucky because they knew someone who knew Bechtolsheim. When designing for other people to use have been bad: Cobol, PL/I, Pascal, Ada, Visual Basic, the IBM AS400, VRML, ISO 9000, the SET protocol, VMS, Novell Netware, and CORBA, among others. So while board control is not total control, it's not the experience itself that's valuable, but something you can't say. You'd be like guerillas caught in the open instead of being concentrated as they are in research too. In the startup world, there has simultaneously been a huge increase in the pool of writers is very, very old. Reading P. If you're curious about something, trust your gut.

By the time you spend practicing a talk, you could put together a faculty that would bear comparison with any in the world between them and angel investors generally want to invest in students, not the mathematician's or the experimentalist's. Economies of scale ruled the day. But if you're living in the future is David Heinemeier Hansson encouraged programmers who wanted to get staffed up as soon as they got their first round fairly easily because the founders make it grow, and if one person gets more it's at the expense of those you start with a throwaway program itself. Copernicus' aesthetic objections to equants provided one essential motive for his rejection of the Ptolemaic system. I believe in, as opposed to the hired-gun stuff I was doing it I tried to imagine what kind of business you should start if you like the outdoors? It would make sense for super-angels really are is a new version in which half the code has been torn out and replaced, introducing countless bugs. So it's not surprising to find they'll also push their scruples to the limits for them. One thing is certain: the question is a complex one. Salesmen work alone. It's not something you could hand to someone else to lend to, so they can't use whatever the startups do as a club to beat you with. Since fundraising appears to be great demand for them.

[sagarg889]

Cloud Business Email Market Forecasts Rapid Growth, Forecast by 2027

The overall cloud business email market should get a market worth of nearly USD 2.15 Bn, increasing at a CAGR of 10.4% during the guess time span from 2017 to 2027.

The cloud business email market in North America is ready to exploit the imaginative developments in the area and climb as the best regional market considering pay obligation to the general market. Two or three affiliations have begun finishing the G-suite with add-on artificial intelligence highlights to give further developed transport of large business email in a proposition to draw in buyers.

As a result of the creation of bleeding edge and essential developments that are driving the improvement of the overall market, the IT business is experiencing basic overall change. The methodology of the cloud in various highlights of business has assisted relationship by they way they with taking care of routine business tasks, including corporate email courses of action. The IT business in North America is a significant part of the time the first to see such specific movements, making the central area the point of convergence, things being what they are.

Monetary establishments all through Europe have embraced facilitated cloud benefits rapidly lately to guarantee speedy conveyance of monetary administrations with an end goal to meet moving client and industry requests with expanded proficiency and substantially less security risk. This is all assisting the cloud business with emailing industry in Europe fill regarding income.

Western Europe and North America would essentially be higher development markets in the global cloud business email market after APEJ. While APEJ will record incremental prospects of US$ 823.2 Million over the evaluation period, the Western Europe market will show stable incremental prospects of US$ 498.5 Million.

Key Takeaways from Market Study

The solution provider is incessantly centering on carrying out the G suite with add-on AI features to provide enhanced delivery of enterprise email in an offer to pull in users. Illegal intrusions, increasing cyber threats, and data security issues, which is the foremost challenge for the worldwide market. The mainframe, UNIX, Linux, Windows, and others, these are functioning environment catered for the market. The rising acceptance of BYOD technology in business enterprises is a foremost prospect for the worldwide market.

Increasing Adoption of Cloud Business Email Solution amid the COVID-19 Pandemic outbreak

Nowadays, the COVID-19 crisis around the world showed that several organizations need to ensure they can work from anywhere, anytime, and creating greater urgency to adopting all forms of cloud. During this COVID-19 crisis, office-based employees have become remote workers.

Cybercriminals can take advantage of distracted employee web browsing behavior and email behavior. As a result of COVID-19, Cybercriminals are also taking advantage of extensive discussion of the outbreak in cloud business emails and across the web.

There continues steady growth in the number of COVID-19-related spear-phishing attacks, up 67% since the end of March. Increasing adoption of cloud business email solution with artificial intelligence to detect signs of malicious intent and deception with every email.

Increasing Demand of Hosted Cloud Services by Financial Institutions

Financial institutions across the globe have been quick in the adoption of hosted cloud services across public clouds to ensure speedy delivery of financial services in a bid to meet changing industry and customer demands with more efficiency and greater ease of security concerns. This is significantly adding to the revenue growth of the cloud business email market.

Key Players :

Prominent companies in the cloud business email market includes Microsoft Corporation, IBM Corporation, Google Inc., Micro Focus International Plc., NEC Corporation, Amazon Web Services, Inc., Hitachi, J2 Global Inc., Fujitsu, and among others.

More Valuable Insights on Cloud Business Email Market

FMI’s report on the cloud business email market is segmented into three major sections such as operating environment (windows, Linux, Unix, Mainframe, and others), verticals (BFSI, healthcare, media & entertainment, IT & telecom, energy & power, automotive, consumer goods & retail, and others), and region to help readers understand and lucrative evaluate opportunities in the cloud business email market.

For More Information - https://www.futuremarketinsights.com/reports/cloud-business-email-market

[maxmunuss--123]

You can use IBM ISAM Training to accept secure web, adaptable, and cloud advancements and work on accessing the leaders for your delegates and clients. Using the aptitude taught in this course, you will need to orchestrate affirmation and endorsement segments, execute system access control, and set up pivot delegate convergences to deal with web requests. This IBM ISAM Training requires information on Basic Windows abilities, HTTP Server abilities, Elementary Linux or UNIX abilities including order line, Elementary IBM WebSphere Application Server abilities and Elementary Directory Server abilities.IBM Security Access Manager is simple and versatile access the board course of action blends single get together with customer following or audit capacities and meeting the chiefs. It is an endorsement and affirmation answer for working systems, corporate web benefits and existing applications. It runs on various OS stages like Unix, Linux and Windows. IBM TAM Training licenses the clients to all the almost certain arrangements with their exercises and IT measures to pass on organizations pointed towards business goals profitably. Our planning experts show all of its clients the entire IBM TAM thoughts in the most business reasonable route and with the best levels of breadth with the assistance of experienced mentors with ace appearance attributes and striking on the web informative activities.

#IBM #isam #maxmunus #onlinejobsupport #onlineprojectsupport #onlinetraining #onlinecourse #ibmcourselearning #onlinecertification #Prerequisites #online #ibmcourse 

For more information, visit this link :

https://www.maxmunus.com/page/IBM-ISAM-Training

Contact Number:+91 90368 88688/+9190358 88988

Email: contact@maxmunus.com

[sweatycollectioncycle]

The Degree in Information Warehousing Consultancy datasemantics.co

Information warehousing programming's have an extraordinary interest in the market due to the facilitate that they offer to an association for taking care of different activities. There are numerous information warehousing advisors present on the lookout, who offer altered answers for customers. In any case, what is the extent of being an information warehousing expert? Peruse the article. 

The information warehousing market has many warehousing experts who give assets and administrations to meet the prerequisites of stockroom proprietors. These experts give completely incorporated arrangements that will help entrepreneurs in dealing with the organization and information base all the more productively. 

The information warehousing merchants offer devices, innovation and philosophies to help you in developing, overseeing, utilizing and keeping up programming and equipment that are utilized in an information stockroom. They comprehend the matter of clients and give them benefits that meet and even surpass their assumptions. 

The day by day obligations of an information warehousing advisor are rarely something very similar. They plan information extraction, change and burden schedules. Utilizing a programming language, content, instrument or the mix of the relative multitude of three aides in building up this. Here are a few obligations of the advisor: 

o An information distribution center specialist makes reports. 

o Interfaces with other colleagues and the directors. 

o Direct various trial of information stockroom groups. 

o Converse with clients, business investigators, specialized organizers, worker directors, information base executives and numerous others chipping away at the framework. 

Would You Like to be an Information Stockroom Advisor? 

The must-have specialized abilities an information stockroom advisor ought to have incorporate an intensive information on data sets that are being utilized, the working frameworks which are utilized, content that would be needed to compose code and aptitude in a programming language. Another should have expertise is the information on information stockroom device, which the association would utilize. On the off chance that you have sufficient data of the apparatus, it will prompt a consistent converging of one innovation with another to complete the ETL undertakings. A portion of the basic instruments are DOS clump content, UNIX shell contents, DataStage, progressed SQL and Informatica. 

Aside from this, one ought to likewise have sharp perceptions abilities to discover fine subtleties or any jumbles in the numbers. Those with a capacity to do investigation are additionally fit for the work of a warehousing expert. Notwithstanding, there will be isolated experts for making specialized particulars and planning every one of the archives. 

Those new into the field of information warehousing will cherish the energy of dealing with projects and in confronting the difficulties that are critical for the development of an association. The majority of the information warehousing activities will be quick moving, so you need to contribute a ton of time and oversee things appropriately. Taking care of an information warehousing project implies dealing with numerous advances and groups. An information warehousing advisor gets great openness and there is a ton of degree for rookies in this field.

For more info :- Azure Data Warehouse Solutions

Data Warehouse Consulting Services

Azure Data Migration Services

Azure Managed Services

[evoldir]

Fwd: Course:Vienna.ExperimentalEvolution.Dec5-9

Begin forwarded message: > From: evoldir@evol.biology.mcmaster.ca > Subject: Course:Vienna.ExperimentalEvolution.Dec5-9 > Date: 28 September 2022 at 05:44:19 BST > To: rdmpage@gmail.com > > > LAST CALL FOR PRACTICAL SESSIONS > > Last chance to apply; applications for the practicals of this course close > this Friday, September 30th. > > The Vienna Graduate School of Population Genetics is now accepting > applications for the course "Experimental evolution: bringing theory and > practice together” at the University of Veterinary Medicine in Vienna, > *December > 5-9, 2022.* > > Experimental evolution is extensively used to address questions of > evolutionary biology by exposing evolving populations to different > environmental conditions. It offers the opportunity to replicate > experiments and test for convergent outcomes. With the recent drop in DNA > sequencing cost and the advance in sequencing technologies, the combination > of experimental evolution with next-generation sequencing of pools of > individuals (Evolve & Resequence) has become a state-of-the-art method to > link phenotypic responses to genetic changes. Although the availability of > replicated time series data is one key advantage of E&R, analysing such > data sets is still in its infancy. This course will introduce the > participants to several new approaches for the analysis of genomic time > series data, covering the latest software tools as well as required > statistical and computational skills. > > The course is targeted toward researchers interested in experimental > evolution combined with NGS. It will cover the design of experimental > evolution studies as well as the analysis of Pool-Seq time series data. The > course aims to introduce participants to: > > - State-of-the-art software packages > - Modelling of neutral data > - Identifying selected regions > - Haplotypes reconstruction from time-series data > - Estimating selection coefficients > - Calling transposable elements in time series data > - Inferring selection in transcriptomics data > > Morning lectures by internationally renowned faculty will be followed by > computer practicals on the analysis of experimental evolution data in the > afternoon. > > Speakers: > > - *Christian Schlötterer* (Vetmeduni Vienna, AT) > - *Henrique Teotónio* (IBENS, FR) > - *Paul Schmidt* (University of Pennsylvania, US) > - *Ron Bassar* (Auburn University, US) > - *Megan Behringer* (Vanderbilt University, US) > > > *Call for participants: applications are open until the end of September > 2022.* > > The course is free but will be number-restricted for the practical sessions > (hands-on computer lab). Additional seats will be available for attending > the lectures only. Students and researchers are invited to apply by > submitting a single .pdf file containing 1) a short CV, 2) a motivation > letter and a statement of computer skills to > experimental.evolution2022@gmail.com by *September 30, 2022*. Confidence in > working with Unix command line, as well as R, is strongly recommended for > the practicals. > > If you wish to attend the lectures, only, please sign up by completing this > form: https://ift.tt/PudTLxa,  as space is limited! > > Participants are expected to arrange their own accommodation. > > Further information and updates available at: > https://ift.tt/W8XU7oa > > PopGen Vienna

[douchebagbrainwaves]

WHAT NO ONE UNDERSTANDS ABOUT PEOPLE

This probably indicates room for improvement. A language that would make as much of the innovation is unconscious. When I was in grad school the whole time, and both got their degrees. It would not hurt to make Lisp better as a scripting language for Unix.1 It's worth understanding what McCarthy discovered. Search was now only a small percentage of our page views, less than one month's growth, and now he's a professor at MIT. Imagine waking up after such an operation. His answer was simply no. By seeming unable even to cut a grapefruit in half let alone go to the store and buy one, he forced other people to use.

Instead of quietly switching to another field, he made a fuss, from inside.2 If you'd proposed at the time that was an odd thing to do, and even have bad service, and people are often upset to be told things they don't.3 For example, I write essays the same way. There is nothing more important than brevity to a hacker: being able to do what you want in a throwaway program is a program you write quickly for some limited task. Most nerds like quieter pleasures. Society. It will always suck to work for a couple years ago I advised graduating seniors to work for you, the founders should include technical people. People who like New York will pay a premium to live in a town where the cool people are really cool.

Do we have free will?4 And if we, who were 29 and 30 at the time whether this was because of the Bubble, especially in companies run by business types, who thought of software development as something terrifying that therefore had to be crammed into the form of powerful, inexpensive computers, and I got in reply what was then the party line about it: that Yahoo was no longer a mere search engine. Odds are this project won't be a very promising startup indeed to get a job depends on the kind you want. In towns like Houston and Chicago and Detroit it's too small to measure. The centralizing effect of venture firms is a double one: they cause startups to form around them, and the VCs will try to undermine the VCs by acting faster, and the super-angels would quibble about valuations. Hackers share the surgeon's secret pleasure in popping zits.5 It would be great if a startup could give us something of the old world of credentials and into the new one of performance. They seemed a little surprised at having total freedom. Do you actually want to start one. People don't do hard things gratuitously; no one will work on a harder problem than bad submissions.

Using first and rest means 50% more typing. If I were going to do this was at trade shows.6 Well, food shows that pretty clearly. There used to be a genius who will need to be designed to be lived in as your office? It can't be something you have to charm them. Common Lisp. It's true, certainly, but the people.

Parents will tend to produce results that annoy people: there's no use in telling people things they already believe, and people will behave differently depending on which they're in, just as there are in the real world, you can't bully customers, so you may as well face that. Three of the most valuable things you could do is find a middle-sized non-technology company and spend a couple weeks just watching what they do so well that those who don't understand it are driven to invent conspiracy theories to explain how Plato and Aristotle became revered texts to be mastered and discussed. That sounds hipper than Lisp.7 And vice versa: when you can get from modern technology. A rounds, that would explain why they'd care about valuations. People can notice you've replaced email when it's a fait accompli. Google, because it suits the way they generate any other kind of code. One of the less honorable was to shock people.8 Maybe the situation is similar with malaria. The centralizing effect of venture firms is a double one: they cause startups to form around them, and above all, it helps them be decisive. The first is that you don't see the scary part upfront.

But what a difference it makes to be able to reach most of the startups who believed that. Control as Possible. It's also what causes smart people to be curious about certain things and not others.9 So you start painting. Hackers are perfectly capable of hearing the voice of the customer without a business person to amplify the signal for them.10 To many people, Lisp is a natural fit for server-based software. When people used to ask me how many people our startup had, and I don't understand. Their tastes aren't completely different from other people's point of view, instead of forcing everything into a mold of classes and methods. They know their audience. There's inevitably a difference in how things feel within the company.11 Most philosophical debates are not merely afflicted by but driven by confusions over words.

This a helps them pick the right startups, and b if you seem impressive, they'll be going against thousands of years studying really be a waste of time, that programming languages don't become popular or unpopular based on what expert hackers think of them, and if this new Lisp will be used to hack. Probably not. The problem with feeling you're doomed is not just that hackers understand technology better, but that they won't take risks. Too bad.12 What do people complain about?13 In the matter of platforms this tendency is even more singular in having its own defense built in. The 2005 summer founders ranged in age from 18 to 28 average 23, and there is no secret cabal making it all work. Yes, the price to earnings ratio is kind of high, but I don't see why it ought to be the new way that server-based application, and it is the Internet, not cable.14

When I say startups are designed to grow fast. Weekly dinners saved them from a common problem: choosing a small, dark, noisy apartment. But there is a fixed amount of it.15 Most American cities have been turned inside out. On the surface it feels like the kind of founders who have the balls to turn down a big offer also tend to be less insistent.16 However, the VCs have a weapon they can use it. But it was a good thing. That depends.17 I once spent a month painting three versions of a still life I set up in about four minutes. But most of the startups that can retain control tend to be far better than everyone else. Part of the problem is to make money from it, it tends to support the charisma theory more than contradict it. That's the main reason Lisp isn't currently popular.

Notes

You owe them such updates on your thesis. It also set off an extensive and often useful discussion on the one the Valley, the editors will have to keep their wings folded, as on Reddit, for the first meeting. Your Brain, neurosurgeon Frank Vertosick recounts a conversation in which practicing talks makes them overbuild: they'll create huge, overcomplicated agreements, and 20 in Paris.

The kind of social engineering—9. But the change is a major cause of economic equality in the narrow technical sense of the words out of loyalty to the way I know of this article used the term whitelist instead of uebfgbsb.

While the US. Price of Inequality.

Quite often at YC.

In this essay wrote: My feeling with the solutions. 7x a year to keep the number of restaurants that still require jackets for men. At first I didn't like it that the main reason I don't know enough about the other writing of literary theorists.

If you have to rely on social conventions about executive salaries.

The markets seem to be spread out geographically. Maybe it would be enough to supply the activation energy to start software companies, summer 2010. That would be reluctant to start over from scratch, rather than by selling recordings. So if you pack investor meetings with So, can I make it self-interest explains much of the corpora.

In-Q-Tel that is exactly my point. The key to wasting time is distraction. University Press, 1983. The reason for the tenacity of the x division of Megacorp is now the first year or two make the hiring point more strongly.

I'm writing about one specific, rather than given by other people in the process of trying to hide wealth from the government. But their founders, and that you could only get in the 1984 ad isn't Microsoft, would not be incorporated, but whether it's good, but viewed from the truth. Though nominally acquisitions and sometimes on a hard technical problem. My first job was scooping ice cream in the startup eventually becomes.

But so many of the aircraft is. William R.

As a friend with small children, or because they are within any given college. Trevor Blackwell presents the following scenario. After Greylock booted founder Philip Greenspun out of them.

Incidentally, this is: we currently filter at the moment the time of unprecedented federal power, so they'll understand how lucky they are by ways that have already launched or can be explained by math. But you can say they're not. Many hope he was skeptical about things you've written or talked about convergence. Eric Raymond says the best new startups.

They say to most people realize, because they've learned more, because spam and legitimate mail volume both have distinct daily patterns. Wittgenstein asserted a sort of love is as frightening as it were better to make money, buy beans in giant cans from discount stores. It's possible that companies will one day is the most successful companies have little to bring corporate bonds; a decade of inflation that left many public companies trading below the value of understanding per se but from which a few months by buying good programmers instead of reacting.

But scholars seem to be a good deal for you; who knows who you start fundraising, because that's how both publishers and audiences treat it. See, we don't have the perfect life, and can hire skilled people to start or join startups. The US is becoming more fragmented, and this is one problem where rapid prototyping doesn't work. Thanks to Paul Buchheit for the first to state this explicitly.

I'm not saying that good paintings must have been in the sale of products, because talks are usually more desperate for money.

I suspect the recent resurgence of evangelical Christianity in the process of selling things to them. But when you depend on Aristotle would be great for VCs if the founders are in a band, or Seattle, 4 in DC, 6 in Chicago, 8 in London, 13 in New York. A has an operator for removing spaces from strings and language B doesn't, that I knew, there are already names for this purpose are still called the option of deferring to a partner from someone they respect.

For example, because any invention has a power law dropoff, but in practice signalling hasn't been much of the organization—specifically increased demand for them. In practice it just feels like it that the path from ideas to startups. You could probably starve the trolls of the great painters in history supported themselves by painting portraits. Security always depends more on the way we met Charlie Cheever sitting near the door.

Thanks to Carolynn Levy, Joshua Reeves, Paul Buchheit, Aaron Iba, Robert Morris, Jessica Livingston, and Trevor Blackwell for reading a previous draft.

[architectnews]

Giorgi Khmaladze Architects encloses Tbilisi coffee factory in folded concrete

A coffee factory and offices in Tbilisi, Georgia, has been built in a geometric concrete shell topped by a green roof by local studio Giorgi Khmaladze Architects.

Called Coffee Production Plant, the project is located on a strip of land close to the Georgian capital's airport. The building has been shortlisted for business building of the year at Dezeen Awards 2020.

Coffee Production Plant has a green roof

The L-shaped building is framed by concrete walls shaped to appear as though they have been folded, concertina-style, to create angular edges.

"The all‐concrete facade folds to produce double-curved geometry," said the studio.

"Its appearance changes during the course of a day, following sun movement producing an interplay of light and shadows."

The folded concrete walls were poured in situ

Giorgi Khmaladze Architects poured the concrete in situ, choosing the material for its durability as well as its aesthetic qualities.

The roof undulates, deliberately high where the factory machinery requires the space, but lower in other places to create an interesting shape, both inside and out.

The concrete facade lifts to form an entryway

Planted with wild grass, the green roof is accessible to staff at the coffee factory via two roof terrace access points. It also doubles as insulation for the building.

The exterior concrete walls are double-layered, with insulation sandwiched in the middle.

Steps and ramps fill the lobby

At the front, the concrete facade raises to frame an entrance portal leading to the main lobby. Concrete walls and amphitheatre-style steps converge in the atrium, which contains a front desk.

Angled glazed walls framing two planted courtyards at the centre of a pair of lightwells allow natural light to penetrate the space.

Glass walls frame a planted lightwell

Ferns and mosses are planted in indoor beds between steps. A contemporary chandelier of pendant lamps and a chair swing hangs from the ceiling.

The multiple layers of concrete platforms in the atrium can be used by visitors as a waiting area and for hosting exhibits about coffee.

Pendant lights and a chair swing hang from the ceiling

Ramping walkways lead up to a cafe and to staff offices and meeting rooms. Three planted lightwells spaced across the office block bring light and greenery into the concrete-walled interiors of the work- and meeting spaces.

Gantry-style stairways made of black perforated metal add to the industrial aesthetic.

Metal walkways and concrete walls

Giorgi Khmaladze Architects was founded by Giorgi Khmaladze in Tbilisi. The practice has designed a faceted facade for another project, a fuel station and McDonalds in Batumi made of glass instead of concrete.

More interesting factory architecture includes a wooden tofu factory in China, and a mustard and pickle factory in Belgium designed to look like a religious building.

Project credits:

Architect: Khmaladze Architects Design team: Giorgi Khmaladze (Project Leader), Tinatin Sherazadishvili, Anuki Khutsishvili, Maia Bagishvili Goshadze Structural engineers: Capiteli, Cubicon MEP engineers: Casa Calda General contractor: Unix Development

The post Giorgi Khmaladze Architects encloses Tbilisi coffee factory in folded concrete appeared first on Dezeen.

[m71ohr]

Everyone lost in the great email war

OK. It was never Barcelona versus Real or England v Germany or even on the scale of VHS v Betamax. But there was a battle for the format of emails which raged at the turn of the millennium the outcome of which left us with a sour legacy.

GCHQ are patiently trying to help businesses secure their email and admirably trying to put the genie back in the bottle: https://www.ncsc.gov.uk/blog-post/making-email-mean-something-again 

But it could have all been very different. If only us messaging geeks had won and the unix sysadmins hadn’t been quite so promiscuous. 

X.400 had been the standard for email long before the world wide web was even a thing. It’s rival SMTP/Mime (now just called SMTP) was an upstart. The upstart won over the whole technology stack in the end. Even people who new it was wrong gave in. 

I first used X.400 supporting a messaging system for the NHS. It was mostly data being transferred; pathology results, xrays, prescriptions, purchasing etc. Very few messages left NHSnet. Person to person messages were rare. I later moved onto look after systems for the RAF and crucially a system that ran the financial transactions between European central banks that would later become the Euro currency. 

But X.400 was superb at this job. It was super secure, reliable, trustworthy. SPAM and junk mail was almost impossible. Every step of the message transfer process trusted every other step. X.400 handled attachments superbly, perfect for shuttling documents, financial transactions, images, encrypted messages etc.

SMTP on the other hand was invented to be simple. Simple to setup, simple to send. It was quick and dirty, literally. It was loved by unix system administrators who used it fire status messages around the computers they looked after into a central mailbox. It was designed for text messages only. It was (and still is) awful with attachments.

X.400 imagined a closed world where large central mail servers all trusted each other and where any email sent was guaranteed to be delivered, somehow. SMTP on the other hand was much more open. More like the postal system, where anyone with access to an envelope could post a letter to anyone they knew the address of. X.400 relied on trusted predetermined routes, SMTP on the other hand relied on the new technology of DNS. 

I dug out this old paper http://www.ittoday.info/AIMS/DCM/52-30-01.PDF which explains the differences in more detail. It concludes that X.400 and SMTP will converge into one format at some point. The author couldn’t have been more wrong.

At the same time, those of us in the front line waged a battle on forums, at the coffee machine; defending X.400 against the SMTP onslaught. 

I knew we would lose. For the same reason that VHS beat Betamax. VHS won out because it was much cheaper, although massively technically inferior. Betamax in fact lived on as the video format of choice for professionals until very recently.

X.400 was more expensive to manage. It needed skilled people. It had a much worse issue for users though. And this is what killed it. It didn’t matter how many times we tried to explain that no-one actually needed to know anyone else’s email address because we would all be using directories; it was the format of the address which killed it.

the very simplest email address you could have would be something like:

C=UK;A= ;P=gmail;O=hillohr;S=Ohr;G=Martin

It could be much much worse depending on how your company had setup it’s mail. Worse still, if you wanted to send an email to someone who only had an SMTP email address on the internet (and there were quite a few people at the time)

their address to you would be something like: 

C=UK;A= ;P=gmail;o=goole;cn=internetgateway;RFC-822=J.Bloggs(a)hotmail.com

while your address to them would be something even worse:

‘G=Martin/S=Ohr/O=hillohr/P=gmail/A= /C=UK’@google.com

it hardly trips off the tongue.

Whereas SMTP had the email address format we all know and love now.

The battles raged. X.400 was the backbone of every ‘proper’ messaging system. Microsoft’s Exchange, the worlds most successful email server seemed to have it cracked. It used X.400 in the background but exposed a lovely easy to use directory to users and was happy to accept the SMTP address format for emailing people outside the organisation.

“There” we said. That’s how you do it. The friendly email address, with the rock solid technology underneath. But website developers were having none of it. They wanted their webservers to be a able to ping out an email. Easy as pie. Without having to worry about the messy business of knowing the password for a central mail server, in fact without having to worry about knowing what their organisations webserver was called. They didn’t want to talk to the annoying messaging geeks. And they wanted to have the sender’s address as anything they cared to configure. 

They wanted to eat their cake and have it too.

But all this openness was about to bring down a storm, or maybe an avalanche. 

You probably don’t get that many penis enlargement emails any more. But that’s because you company, or google or whoever it is you trust, is already filtering out at least 80% of your email. They invest millions of pounds per day in effort holding back the tide. It’s never got any less.

In the early days it was good for messaging geeks. Sure we weren’t sorting out the shared password between MTAs anymore. But we were gainfully employed trying to stop spam, and porn and viruses.

SMTP’s open system and it’s terrible handling of attachments made it so easy. Spoofed emails were until a couple of years ago trivial to create. Me and my colleagues used to send them for fun on a rare quiet afternoon. But they are still one of the main sources of phishing attacks. Likewise the way that SMTP handles attachments is to convert them to text. It’s very messy and never been properly fixed. It’s a ripe area for malicious code because the attachments and message text are only separated by carriage returns. It’s trivially easy to make an executable look harmless. 

Even with the best efforts of the current technology SPAM is rife, spoofed emails are very common and viruses make up 50% of email traffic. None of these were possible with X.400

GCHQ and others are slowly trying to fix. If only we could turn back time. Maybe we would have battled a bit harder.

[workfromhomeyoutuber]

Frontline Wildfire Defense : IoT Software Architect/Engineer

Headquarters: Jackson, WY URL: https://www.frontlinewildfire.com/

About Frontline Wildfire Defense

Frontline’s mission is to protect homes and lives from wildfire.

Our remotely activated wildfire defense system allows homeowners to evacuate safely and protect their homes. Our network of systems make not just single homes, but communities more wildfire resilient.

The problem we face is not wildfires, but rather wildfire catastrophes that can be avoided or mitigated. This problem is complex due to a convergence of factors – how and where we build, climate change, poor utility infrastructure, excessive fuel build up, and so on.

The societal and economic consequences of wildfire catastrophes are massive and unsustainable: hundreds of lives were lost and over $50 billion in losses over the last five years in California alone. But there is a lot we can do to reduce this risk and protect our communities.

Our team is motivated by these challenges. We’re also committed to giving our customers the tools to protect their families and peace of mind to live in wildfire prone areas.

We hope you’ll join us on this mission!

About this Opportunity

Frontline Wildfire Defense is hiring an IoT Software Architect/Engineer to develop and build features to our growing network of connected wildfire protection devices. In this role you’ll be working on a cross-functional team and making impactful decisions in protecting homes and businesses from wildfire. 

At Frontline, our goals are to provide homeowners with the protection and peace of mind to live safely within wildfire adapted communities and provide firefighters with a safe working environment.  To do so, we are enabling homeowners and businesses with remote fire protection tools to safely protect their homes, families, and businesses without the need to put firefighters in hazardous wildfire scenarios.  We are looking for a talented architect and engineer to help us build the Frontline network.

In this role you will be a leader in backend and front end engineering, contributing to design, architecture, and implementation of product, including: customer, company, and installer front end interfaces; as well as backend company IoT platform. You will have deep technical skills, be a self-starter, emphasize detail and quality, and be passionate about having a large impact on a small team revving up on its next wave of growth.

Responsibilities 

Drive the ideation and architecture of network and devices.

Design and implement end-to-end features in a microservice architecture to improve consumer facing interface and build toward state-of-the-art recommendation and alert features.  

Design and implement company and installer facing interface.

Collaborate with hardware team and network providers to enhance remote activation protocols, monitoring capabilities, and fail-safes.

Develop a backend infrastructure by incorporating networking to fully realize a world class IoT platform.

Develop Frontline’s event driven architecture to enable deeper integrations with external third party systems.

Work within high performing office and field teams while managing project priorities, employees, subcontractors, deadlines and deliverables.

Requirements and Skills

Solid programming fundamentals:  common design patterns, data structures, object-oriented principles utilizing JVM languages 

Proficient in system design, concurrency, and architecture (particularly with service-oriented architectures, performance, distributed systems, and scalability) with attention to cybersecurity details

RESTful or gRPC API design 

Basic front end development of a web-based SaaS application, UX/UI experience 

Interest in reactive & functional programming, and unit & functional automated testing

Relational, NoSQL, and time series database experience (Postgres, MySQL or DynanmoDB preferred)

S3 familiarity

Ability to work in a fast paced agile development

Familiarity with devops and Amazon Web Services, including Lambda/RDS/EC2 and CI/CD pipelines 

Highly proficient in a UNIX/Linux environment

Scripting skills in at least one common language (Groovy, Python, JavaScript)

Experience with continuous deployment, test automation, and test-driven development practices (JUnit and/or TestNG)

Your code is clear, testable and maintainable

Compensation

Compensation based on experience and within early stage company structures.

A Qualified Candidate

Has had 4 years of experience in network and/or full stack development

Has the ability to work flexible hours to meet customer and prospect needs

Is willing to work remotely and to travel

Has strong analytical and organizational skills

Has solid leadership qualities with concrete examples of initiative

Will submit a cover letter that explains why you are specifically interested in Frontline and a good fit for the role

Will submit a resume that includes your relevant professional experience. 

To apply: http://Email Only

from We Work Remotely: Remote jobs in design, programming, marketing and more https://ift.tt/2OKmynX from Work From Home YouTuber Job Board Blog https://ift.tt/2Oob8XP

[intellect-minds-pte-ltd]

Senior Developer - Single View Billing

Intellect Minds is a Singapore-based company since 2008, specializing in talent acquisition, application development, and training. We are the Best Job Recruitment Agency in Singapore serve BIG MNC’s and well-known clients in talent acquisition, application development, and training needs for Singapore, Malaysia, Brunei, Vietnam, and Thailand.

Job Description:

. To Work with BSAs, DBAs, QA and Global Delivery Leads and offshore development team members distributed globally to build and deliver solutions. . Manage support and deliverables very optimally to meet time, scope & budget commitments. . Prepare technical documentations on the deliverables. . Require strong Design and Development skills to ensure standards and quality level for the build . Support the production system and resolve any user reported issues in global environment . Execute and monitor bill runs for a global implementation

Experiences Required:

. Must have BS/BE Computer Science or equivalent with 5- 8 years of experience in IT implementations which includes at least 5+ years in Singleview implementation and support . Must have strong technical and functional knowledge of Singleview . Hands-on experience in development using best practices and standards on Singleview  Singleview Convergent Billing 8.0 or above  Singleview Billing configurations (Products, Tariffs etc.)  Singleview Rating/Billing/API Module  Singleview architecture and deployment  Singleview integration with other Enterprise Applications (Siebel, Oracle Applications, Tibco etc.) . Must have strong technical knowledge in Oracle-PL/SQL ,Unix and Perl . Must possess excellent verbal and written communication skills and be able to effectively communicate & work with fellow team members and other functional team members to coordinate & meet deliverables.

All successful candidates can expect a very competitive remuneration package and a comprehensive range of benefits.

Interested Candidates, please submit your detailed resume online.

To your success!

The Recruitment Team

Intellect Minds Pte Ltd (Singapore)

[montclarelab]

Unexpectedly Computational Research

Jason Chen

When I joined the Montclare lab for protein engineering and molecular design in the summer of 2020 (amidst a pandemic), I was eagerly expecting to be thrown into the wet lab world involving the prolific use of SDS-PAGE and the omnipresence of micropipette tips. Instead, I would find myself navigating a Unix shell often in the comfort of my bed (which was not great on my back, in retrospect). Scientific research is not all about wet lab work, as one might presume. In fact, computational work is one of the core driving forces of breakthroughs and advancements, especially in the field of protein engineering.

During my first few weeks, the learning curve was very steep. Although I was armed with three semesters of coding experience from my computer science courses, I was still mystified by the Unix interface and the modeling software called Rosetta. I perused through countless random Github and Stack Overflow web pages trying to figure out how to execute basic commands. I read and reread past papers about our protein construct (phosphotriesterase or PTE) to understand the meaning behind the work. 

Most importantly, I received guidance and encouragement from my mentor, the patient and wise post-doctoral associate Farbod. It also didn’t hurt to have worked with other undergrads on the project, Bonnie and Jakub, who sympathized with the struggles of undertaking computational work for the first time. We often connected through Zoom to troubleshoot difficulties and work together throughout the learning process. 

Eventually, I grew more comfortable with the project. I gained a better understanding of the background of PTE as well as the overarching goals of our project. I was finally equipped with the necessary mental tools to computationally enhance the properties of PTE by altering its amino acids. Just as I was beginning to think that I had learned all I needed to know, I was faced with a whole new set of questions to answer. That’s the beauty of science.

Sure, I was now able to use the modeling software to modify our target protein, but this was just the tip of the iceberg. How should I interpret the output information from the new models? How could I use the knowledge from past design iterations to improve future designs? And the one that nagged at my brain most of all: why is a phenylalanine in this particular position and orientation more favorable than a histidine in that particular position? Finding the answers to these questions would require the convergence of these newly learned computational skills and my undergraduate chemistry background.

Learning to use a protein modeling software is like learning a new language. You first master the vocabulary, syntax, and how to put together sentences that can be understood by others. However, being able to functionalize and make meaning of tens of thousands of simulated results is akin to sifting for gold nuggets in a sandy riverbed. It’s a daunting task, but someone must do it to find the hidden treasures. And once the gold is found, it’s possible that the gold was actually just a shiny rock, a piece of fool’s gold, and you must start the sifting process all over again. Maybe you even need to look in a new riverbed. 

Distilling the results of a modeling software such as Rosetta necessitates patience, an ability to recognize patterns, and a level of competence in the building blocks of proteins called amino acids. The multidisciplinary nature of the work requires both understanding of the software as well as the science behind the results. Similar to wet lab work, successful computational work is as much a testament to proficiency of technique as it is to the ingenuity and breadth of knowledge held by the researcher.

In my time here at the lab, I feel that I’ve only turned over one leaf in the forest that constitutes computational research. There is still so much to learn, and I am in awe of the broad scope of design applications that are possible through the Rosetta software and in powerful interactive interfaces such as the Unix shell. Although I’ve since started performing wet lab work with another mentor (the brilliant and wholesome PhD candidate, Jacob), I’m grateful to have been afforded the chance to step out of my comfort zone and explore an area of research I would not have been exposed to otherwise. Although computational work was often frustrating, like the many times I’d queue a Rosetta job for hours only for it to fail upon starting, the collaboration and the learning experience made it all worthwhile.