#protoplast

[cytgen]

Abstract The members of the Brassicaceae family are important oilseed, vegetable and forage crops. The Brassicaceae species are also used for phytoremediation of heavy metal polluted soils, as siderates or ornamental plants. The widening of biodiversity of already existent Brassicaceae cultivars by genetic engineering manipulations can increase tolerance to biotic and abiotic stresses, increase plant productivity and production quality. Protoplast fusion method makes possible artificial synthesis of various somatic hybrids between different plant species. The review presents advances in protoplast fusion of Brassica crops and their wild relatives; main directions of somatic hybridisation in Brassicaceae; the conditions for obtaining and selection of the somatic hybrids; the peculiarities of genetic material inheritance, introgression of economically important traits by somatic hybridization to cultivated Brassicaceae, resynthesis of allotetraploid species; production of cytoplasmic male-sterile (CMS) alloplasmic lines, creation of somatic hybrids for phytoremediation of soils contaminated with heavy metals, and production of transplastomic plants by transfer of genetically modified plastids through protoplast fusion.

[whats-in-a-sentence]

Three developmental stages of zinnia xylogenesis have been identified, each associated with specific physiological states, morphological changes, and gene expression patterns:

Stage I consists of the de-differentiation of mesophyll cells and the acquisition of competence to re-differentiate into tracheary elements. De-differentiation involves many of the same genes involved in the wound response of plants.

Stage II, the synthesis, patterning, and deposition of secondary wall material begin. Autophagy becomes active at this time, contributing to cell autolysis.

In Stage III, the deposition of secondary wall thickening is completed and lignification takes place. Simultaneously, the vacuolar membrane breaks down, which leads to complete protoplast autolysis, including the plasma membrane. At the end of the process, only a hollow tube consisting of an outer granular layer, a middle layer of primary cell wall, and an inner layer with lighted secondary wall thickenings remains (Figure 18.33).

For example, vessel elements form in zinnia suspension-cultured cells have closed end walls (see Figure 18.33A-E), in contrast to vessel elements formed in vivo, which have open end walls. (...) Xylogen normally accumulates in the meristem, procambium, and xylem of zinnia seedlings, and is concentrated at the apical ends of the cell walls of differentiating tracheary elements (Figure 18.33F).

"Plant Physiology and Development" int'l 6e - Taiz, L., Zeiger, E., Møller, I.M., Murphy, A.

[tenth-sentence]

A pulse of blue light given under a saturating red-light background can also stimulate an outward electric current from guard cell protoplasts (Figure 10.7B).

"Plant Physiology and Development" int'l 6e - Taiz, L., Zeiger, E., Møller, I.M., Murphy, A.

[flawlessflesh]

cecil is the thistle expy i made for a roleplay group i joined! he's started taking on a life of his own though...

in this rp, the mundane world and the supernatural secretly co-exist. cecil lives with his family in an underground bunker deep within washington state's hoh rainforest. the family is locked away and cecil believes he's protecting them from harm. he spends his days digging a frighteningly complex system of tunnels throughout the forest, convinced that if he can reach the black river deep inside the earth that's haunting his dreams, he'll be able to find his brother del's missing soul.

^ cecil being hounded by a good samaritan (samson's dorothy <3)

i'll share the public bio i wrote for cecil under the cut hehe

Cecil of the Talayi family began appearing in New Portsmouth grocery stores and libraries a few months ago. He isn't occupied by any work or schooling and he disappears for long stretches of time into the Hoh Rainforest.

A receipt Cecil discarded shows the following purchases: a wheelbarrow, five 50-pound bags of concrete mix, gloves, a large pack of 2x4 lumber, steel pipes, an industrial saw, and a bag of sour gummy worms (berry blast flavor).

When he was a young child, Cecil was given a home by the Talayi family. There is no official adoption on record and it is unknown exactly when Cecil was brought in. The family's head at the time, Farhang (فرهنگ), was an Iranian immigrant who made his fortune in Silicon Valley - Cecil believes he owes everything to him. Cecil was homeschooled alongside his younger brother, Del (عادل), who he helped raise. These days he is his brother's primary caretaker.

Farhang Talayi was assassinated by poisoning in 1991 by suspected corporate rivals. The murder trial was long, arduous, and inconclusive. Contemporary newspaper articles and tv footage of the investigation are archived online.

Ten years after the trial, the Talayi family moved from the bustle of Silicon Valley to New Portsmouth. Del bought property deep in the forest and built a private compound ringed by high walls. The Talayis made a few half-hearted excursions into the town's social scene, but they kept to themselves for the most part, homeschooled their children, and now have not been seen in public for many months.

Those initiated in the Secret World will look at Cecil and know he is a Protoplast - a name given to an insular race of humans whose proximity to Flesh diverged them from Homo sapiens thousands of years ago. Not much is known about Protoplasts beyond their natural skill with the Flesh font and their unusual physical characteristics: androgyny, elongated goat-like ears, hair and eyes lacking melanin, and rumors of extended lifespans. They are viewed with awe and wariness - anyone so close to Flesh should be handled carefully, of course. Most Protoplasts seen outside their clans are in the employ of the rich and powerful, a living status symbol. Cecil is no different.

[megalizardon4736]

Zoe Patel Makeover 💜

Defaults -

Beetle Eyes 2 by squeamishsims

No EA Lashes by Kijiko

Hair -

Yennefer Hair v2 by manul-sul-sul

Teresa Hair by sehablasimlish (thesimsresource)

Artemis Hair by qicc (thesimsresource)

Eyebrows -

Updated Stretchskeleton Eyebrows by whoismae

Eyes -

Contacts #68 by sims3melancholic

Eyelash v4 by MMSIMS

Skin Details -

Skin n6 Overlay by northern siberia winds

FEMALE TORSO MASK N3 MM OVERLAY by northern siberia winds

Nose Masks by RemusSirion (thesimsresource)

Nose Tip Nose Mask by obscurus-sims

Moomoo Blush by squeamishsims

Blush n8 Lips by northern siberia winds

Makeup -

Nail It! Nail Set 2.0 by Joliebean

Kendell Eyeshadow by crypticsim

Saffron Liner by crypticsim

Neutrals vol 1 eyeshadow by crypticsim

Blush n8 by poyopoyo

Sour Fruit Lipstick by TwistedCat

Wedding Lipstick by TwistedCat

Shell Dust Eyeshadow by faaeish

Protoplast Lipstick by RemusSirion (thesimsresource)

Vanilla (soft) n02 Eyeshadow by Pralinesims

Jinsoul Lipstick by crypticsim

Clothes -

Love Blossom Earrings by Pralinesims

Ultimate CAS items Unlocker by Loulicorn

Adam Set Ripped Jeans by helgatisha

Alex Sneakers by arethabee

Reverse Reverse Bracelets by AJ Nebula

Shine Bright Dangly Earrings by ghostputty

Aarnes Gown by Rusty's

Olivia Gloves by Sentate

qt headphones v2 by arethabee

Lena Top by arethabee

Booty Bike Shorts by Caio

Extreme Tights by Joliebean

Moonlight Sneakers by Trillyke

Dreams Jumpsuit by Caio

Kitty Choker by Blue Craving

Pentagram Earrings by Blue Craving

K-pop Off Shoulder Dress by HappyLifeSims

Stockings by Sentate

Sakura High Boots by Serenity

Siren Song One Piece Swimsuit by Joliebean

Jessie Dress v2 by arethabee

Clover's Cozy Coat by Nolan-Sims

Woody Jeans by Rusty's

@remussirion @obscurus-sims @squeamishsims @joliebean

@twisted-cat @pralinesims @crypticsim @northernsiberiawinds

@manul-sul-sul @trillyke @nolan-sims @rustys-cc @serenity-cc

@helgatisha @happylifesims @arethabee @caio-cc @sentate

@loulicorn @bluecravingcc

[spacelazarwolf]

But what is the cutest plant? The baby-est baby?

so this is most definitely not the answer you were probably expecting but my answer is lil baby tissue culture plants.

plant tissue culture is defined as ulturing plant seeds, organs, explants, tissues, cells, or protoplasts on a chemically defined synthetic nutrient media under sterile and controlled conditions of light, temperature, and humidity. what you get are things like this:

they are so tiny and cute and they are a great way to add more sought after plants to your collection without breaking the bank. it's also a very rewarding process to grow a plant from a teeny tiny baby to a fully grown plant.

[puppy--jam]

SVTFOE Timeline headcanon

Based on this

I can state that according to the Mewni calendar, the events of the series take place in 1753 (although S1 and S2 may have taken place in 1752, but that's not important). Just as on Earth, so on Mewni there is a marker of eras, namely the Mewnipendance Day, which is the first year.

Assuming Mewnipendance Day is year 1, over 1750 years have passed with 38 queens reigning… My headcanon, so I can interpret it as I want. To make the dates fit nicely, I chose 12th Mewni Queen for a long-lived mewman (I'll describe her later, as she's important in my AU) and extended Celena's life (since Celena is/was half-demon).

Interesting fact: Time on Mewni/Serparsis (generally the planet where these continents are located) flows about twice as fast as on Earth. That is, when the Butterflies began their rule, it was the Middle Ages on Earth.

Au History timeline (will include oc queens):

around -150 (Yes, Mewmans use '-' instead of BC [Before Christ]) -> the beginning of human migration to Mewni (carried out by Glossaryck).

1 -> Mewnipendance Day (Great Monster Massacre)

2-14 -> Moe (the Zero) the Protoplast's reign

14-70 -> first queen's reign (it's that baby with the circles on her cheek)

70-72 -> Soupina the Strange's reign (she reigned for a short time because she went mad due to the mewberty form. [I will add that the first queen did not achieve this form]. Then it was decided, in order to better prepare for the use of magic, the wand would be passed on on the 14th birthday to the next descendant to the throne)

72-114 -> third queen's reign ; 114-137 -> 4th queen's reign ; 137-172 -> 5th queen's reign ; 172-194 -> 6th queen's reign ; 194-215 -> 7th queen's reign ; 215-231 -> 8th queen's reign ; 231-253 -> 9th queen's reign ; 253-273 -> 10th queen's reign ; 273-294 -> 11th queen's reign

294-1119 -> 12th queen's reign, Asynthia the Darkest One (I wil write post about her somewhen)

1119-1141 -> 13th queen's reign. Her title was the Reviver (I will mention that during her reign, inheritance to the throne was introduced at the age of 25)

1141-1168 -> 14th queen's reign ; 1168-1181-> Tituba the Seeker (15th)(character is based on Tituba, the alleged witch who was involved in the Salem trials)

1181-1219 -> 16th queen's reign ; 1219-1240-> 17th queen's reign ; 1240-1258-> 18th queen's reign ; 1258-1281-> 19th queen's reign ; 1281-1299-> 20th queen's reign ; 1299-1323-> 21st queen's reign

1323-1356-> Solena the Snitten (22nd) (during her time there was a conflict with the Lucitors, which Solaria mentioned in her chapter)

1356-1380-> 23rd queen's reign ; 1380-1406-> 24th queen's reign ; 1406-1431-> 25th queen's reign ; 1431-1443-> Lyric (26th) the Untrust's reign ; 1443-1481-> (27th) Skywynne's reign ; 1481-1499-> (28th) Solaria's reign ; 1499-1510-> (29th) Eclipsa's first reign ; 1510-1514-> interregnum, MHC ruled over kingdom ; 1514-1558-> (30th) Festivia's reign ; 1558-1589-> (31st) Cresenta's reign ; 1589-1615-> (32nd) Rhina's reign ; 1615-1691-> (33rd) Celena's reign ; 1691-1721-> (34th) Esterlla's reign ; 1721-1727-> (35th) Comet's reign ; 1727-1753-> (36th) Moon's reign ; 1753-> (37th) Star's four day reign ; 1753-> (38th) Eclipsa's second reign

And on the end I will mention Stump Day. This is something of a legend. When the first people arrived on Mewni (around -150), the Butterfly family celebrates it as their holiday (probably one of the queens instituted it).

[rangercorpstherapy]

As for those condoms (bringing this topic up in this fandom was NOT in my bingo for this week)

Obviously, their origins dated back to very ancient Egypt and in the Middle Ages there was already some sort of protoplast of them

But

1. they were not generally available to ordinary people

2. it's not like they actually worked, made of leather/animal skin or fine material (a bit more like a heater and than what we have today)

x

[whats-in-a-sentence]

In the laboratory, guard cell protoplasts swell when illuminated with blue light (Figure 10.5), indicating that blue light is sensed within the guard cells proper. (...) This blue light-induced acidification is blocked by uncouplers that dissipate pH gradients, such as CCCP (discussed shortly), and by inhibitors of the proton-pumping H+-ATPase (discussed in Chapter 6), such as orthovanadate (see Figure 10.5B).

"Plant Physiology and Development" int'l 6e - Taiz, L., Zeiger, E., Møller, I.M., Murphy, A.

[mindofasnail]

collected notes from Dec 3rd, 2023 Shadowjam at Hypha Arts

themes: forces of nature, FUNd art not war, intergenerational starry hippos, whimsical whistleblowers, among other things...

sip sip stormy lightning

PLACENTAPEDE!

the mean king/the mute king/the four horned king

inside the mind face of the silent king

bow down, bow wow wow

marty macdonald brow was very hungry

angry meal

the avatar

weird blue haired one

i need to feel electricity

hold my hand

viewing window in the hill of gestation

twins for sale

the twins had no names, they were offended

"be careful who you tell our name to"

commerce was the midwife

dna evolution

THE BIRTH OF MODERNITY

PUT IT BACK!!!

build it up

cluttered modernity

pandoras stinky socks

day night day night day night

rapid flooding water rising falling

the falling person in the swirl

PROTOPLASTIC/PROTOPLASMIC PEOPLE!

lightbulb bird

flyby angel

alligator zipping through

tickle my ears

pointed teeth

perforate the paper of the pulp and paper planet

you can't force the sun out of the sky

ghost conference

in hindsight the afterlife was not what i expected

[rjalker]

The Jovian Jest, by Lilith Lorraine, originally published in May 1930. Public domain. Word count: 2,861 Horror, comedy, science fiction

= = =

Consternation reigned in Elsnore village when the Nameless Thing was discovered in Farmer Burns' corn-patch. When the rumor began to gain credence that it was some sort of meteor from inter-stellar space, reporters, scientists and college professors flocked to the scene, desirous of prying off particles for analysis. But they soon discovered that the Thing was no ordinary meteor, for it glowed at night with a peculiar luminescence. They also observed that it was practically weightless, since it had embedded itself in the soft sand scarcely more than a few inches.

By the time the first group of newspapermen and scientists had reached the farm, another phenomenon was plainly observable. The Thing was growing!

Farmer Burns, with an eye to profit, had already built a picket fence around his starry visitor and was charging admission. He also flatly refused to permit the chipping off of specimens or even the touching of the object. His attitude was severely criticized, but he stubbornly clung to the theory that possession is nine points in law.

It was Professor Ralston of Princewell who, on the third day after the fall of the meteor, remarked upon its growth. His colleagues crowded around him as he pointed out this peculiarity, and soon they discovered another factor—pulsation!

Larger than a small balloon, and gradually, almost imperceptibly expanding, with its viscid transparency shot through with opalescent lights, the Thing lay there in the deepening twilight and palpably shivered. As darkness descended, a sort of hellish radiance began to ooze from it. I say hellish, because there is no other word to describe that spectral, sulphurous emanation.

As the hangers-on around the pickets shudderingly shrank away from the weird light that was streaming out to them and tinting their faces with a ghastly, greenish pallor, Farmer Burns' small boy, moved by some imp of perversity, did a characteristically childish thing. He picked up a good-sized stone and flung it straight at the nameless mass!

Instead of veering off and falling to the ground as from an impact with metal, the stone sank right through the surface of the Thing as into a pool of protoplastic slime. When it reached the central core of the object, a more abundant life suddenly leaped and pulsed from center to circumference. Visible waves of sentient color circled round the solid stone. Stabbing swords of light leaped forth from them, piercing the stone, crumbling it, absorbing it. When it was gone, only a red spot, like a bloodshot eye, throbbed eerily where it had been.

Before the now thoroughly mystified crowd had time to remark upon this inexplicable disintegration, a more horrible manifestation occurred. The Thing, as though thoroughly awakened and vitalized by its unusual fare, was putting forth a tentacle. Right from the top of the shivering globe it pushed, sluggishly weaving and prescient of doom. Wavering, it hung for a moment, turning, twisting, groping. Finally it shot straight outward swift as a rattler's strike!

Before the closely packed crowd could give room for escape, it had circled the neck of the nearest bystander, Bill Jones, a cattleman, and jerked him, writhing and screaming, into the reddish core. Stupefied with soul-chilling terror, with their mass-consciousness practically annihilated before a deed with which their minds could make no association, the crowd could only gasp in sobbing unison and await the outcome.

The absorption of the stone had taught them what to expect, and for a moment it seemed that their worst anticipations were to be realised. The sluggish currents circled through the Thing, swirling the victim's body to the center. The giant tentacle drew back into the globe and became itself a current. The concentric circles merged—tightened—became one gleaming cord that encircled the helpless prey. From the inner circumference of this cord shot forth, not the swords of light that had powdered the stone to atoms, but myriads of radiant tentacles that gripped and cupped the body in a thousand places.

Suddenly the tentacles withdrew themselves, all save the ones that grasped the head. These seemed to tighten their pressure—to swell and pulse with a grayish substance that was flowing from the cups into the cord and from the cord into the body of the mass. Yes, it was a grayish something, a smokelike Essence that was being drawn from the cranial cavity. Bill Jones was no longer screaming and gibbering, but was stiff with the rigidity of stone. Notwithstanding, there was no visible mark upon his body; his flesh seemed unharmed.

Swiftly came the awful climax. The waving tentacles withdrew themselves, the body of Bill Jones lost its rigidity, a heaving motion from the center of the Thing propelled its cargo to the surface—and Bill Jones stepped out!

Yes, he stepped out and stood for a moment staring straight ahead, staring at nothing, glassily. Every person in the shivering, paralysed group knew instinctively that something unthinkable had happened to him. Something had transpired, something hitherto possible only in the abysmal spaces of the Other Side of Things. Finally he turned and faced the nameless object, raising his arm stiffly, automatically, as in a military salute. Then he turned and walked jerkily, mindlessly, round and round the globe like a wooden soldier marching. Meanwhile the Thing lay quiescent—gorged!

Professor Ralston was the first to find his voice. In fact, Professor Ralston was always finding his voice in the most unexpected places. But this time it had caught a chill. It was trembling.

"Gentlemen," he began, looking down academically upon the motley crowd as though doubting the aptitude of his salutation. "Fellow-citizens," he corrected, "the phenomenon we have just witnessed is, to the lay mind, inexplicable. To me—and to my honorable colleagues (added as an afterthought) it is quite clear. Quite clear, indeed. We have before us a specimen, a perfect specimen, I might say, of a—of a—"

He stammered in the presence of the unnamable. His hesitancy caused the rapt attention of the throng that was waiting breathlessly for an explanation, to flicker back to the inexplicable. In the fraction of a second that their gaze had been diverted from the Thing to the professor, the object had shot forth another tentacle, gripping him round the neck and choking off his sentence with a horrid rasp that sounded like a death rattle.

[Image description start: A black and white illustration, showing the glowing sphere reaching out a tentacle to grab the suited professor, while the crowd watches on in horror. Image description end.]

Needless to say, the revolting process that had turned Bill Jones from a human being into a mindless automaton was repeated with Professor Ralston. It happened as before, too rapidly for intervention, too suddenly for the minds of the onlookers to shake off the paralysis of an unprecedented nightmare. But when the victim was thrown to the surface, when he stepped out, drained of the grayish smokelike essence, a tentacle still gripped his neck and another rested directly on top of his head. This latter tentacle, instead of absorbing from him, visibly poured into him what resembled a threadlike stream of violet light.

Facing the cowering audience with eyes staring glassily, still in the grip of the unknowable, Professor Ralston did an unbelievable thing. He resumed his lecture at the exact point of interruption! But he spoke with the tonelessness of a machine, a machine that pulsed to the will of a dictator, inhuman and inexorable!

"What you see before you," the Voice continued—the Voice that no longer echoed the thoughts of the professor—"is what you would call an amoeba, a giant amoeba. It is I—this amoeba, who am addressing you—children of an alien universe. It is I, who through this captured instrument of expression, whose queer language you can understand, am explaining my presence on your planet. I pour my thoughts into this specialised brain-box which I have previously drained of its meager thought-content." (Here the "honorable colleagues" nudged each other gleefully.) "I have so drained it for the purpose of analysis and that the flow of my own ideas may pass from my mind to yours unimpeded by any distortion that might otherwise be caused by their conflict with the thoughts of this individual.

"First I absorbed the brain-content of this being whom you call Bill Jones, but I found his mental instrument unavailable. It was technically untrained in the use of your words that would best convey my meaning. He possesses more of what you would call 'innate intelligence,' but he has not perfected the mechanical brain through whose operation this innate intelligence can be transmitted to others and, applied for practical advantage.

“Now this creature that I am using is, as you might say, full of sound without meaning. His brain is a lumber-room in which he has hoarded a conglomeration of clever and appropriate word-forms with which to disguise the paucity of his ideas, with which to express nothing! Yet the very abundance of the material in his storeroom furnishes a discriminating mind with excellent tools for the transportation of its ideas into other minds.

"Know, then, that I am not here by accident. I am a Space Wanderer, an explorer from a super-universe whose evolution has proceeded without variation along the line of your amoeba. Your evolution, as I perceive from an analysis of the brain-content of your professor, began its unfoldment in somewhat the same manner as our own. But in your smaller system, less perfectly adjusted than our own to the cosmic mechanism, a series of cataclysms occurred. In fact, your planetary system was itself the result of a catastrophe, or of what might have been a catastrophe, had the two great suns collided whose near approach caused the wrenching off of your planets. From this colossal accident, rare, indeed, in the annals of the stars, an endless chain of accidents was born, a chain of which this specimen, this professor, and the species that he represents, is one of the weakest links.

"Your infinite variety of species is directly due to the variety of adaptations necessitated by this train of accidents. In the super-universe from which I come, such derangements of the celestial machinery simply do not happen. For this reason, our evolution has unfolded harmoniously along one line of development, whereas yours has branched out into diversified and grotesque expressions of the Life-Principle. Your so-called highest manifestation of this principle, namely, your own species, is characterized by a great number of specialized organs. Through this very specialization of functions, however, you have forfeited your individual immortality, and it has come about that only your life-stream is immortal. The primal cell is inherently immortal, but death follows in the wake of specialization.

“We, the beings of this amoeba universe, are individually immortal. We have no highly specialized organs to break down under the stress of environment. When we want an organ, we create it. When it has served its purpose, we withdraw it into ourselves. We reach out our tentacles and draw to ourselves whatsoever we desire. Should a tentacle be destroyed, we can put forth another.

"Our universe is beautiful beyond the dreams of your most inspired poets. Whereas your landscapes, though lovely, are stationary, unchangeable except through herculean efforts, ours are Protean, eternally changing. With our own substance, we build our minarets of light, piercing the aura of infinity. At the bidding of our wills we create, preserve, destroy—only to build again more gloriously.

"We draw our sustenance from the primates, as do your plants, and we constantly replace the electronic base of these primates with our own emanations, in much the same manner as your nitrogenous plants revitalize your soil.

"While we create and withdraw organs at will, we have nothing to correspond to your five senses. We derive knowledge through one sense only, or, shall I say, a super-sense? We see and hear and touch and taste and smell and feel and know, not through any one organ, but through our whole structure. The homogeneous force of our omni-substance subjects the plural world to the processing of a powerful unity.

“We can dissolve our bodies at will, retaining only the permanent atom of our being, the seed of life dropped on the soil of our planet by Infinite Intelligence. We can propel this indestructible seed on light rays through the depths of space. We can visit the farthest universe with the velocity of light, since light is our conveyance. In reaching your little world, I have consumed a million years, for my world is a million light-years distant: yet to my race a million years is as one of your days.

"On arrival at any given destination, we can build our bodies from the elements of the foreign planet. We attain our knowledge of conditions on any given planet by absorbing the thought-content of the brains of a few representative members of its dominant race. Every well-balanced mind contains the experience of the race, the essence of the wisdom that the race-soul has gained during its residence in matter. We make this knowledge a part of our own thought-content, and thus the Universe lies like an open book before us.

"At the end of a given experiment in thought absorption, we return the borrowed mind-stuff to the brain of its possessor. We reward our subject for his momentary discomfiture by pouring into his body our splendid vitality. This lengthens his life expectancy immeasurably, by literally burning from his system the germs of actual or incipient ills that contaminate the blood-stream.

“This, I believe, will conclude my explanation, an explanation to which you, as a race in whom intelligence is beginning to dawn, are entitled. But you have a long road to travel yet. Your thought-channels are pitifully blocked and criss-crossed with nonsensical and inhibitory complexes that stand in the way of true progress. But you will work this out, for the Divine Spark that pulses through us of the Larger Universe, pulses also through you. That spark, once lighted, can never be extinguished, can never be swallowed up again in the primeval slime.

"There is nothing more that I can learn from you—nothing that I can teach you at this stage of your evolution. I have but one message to give you, one thought to leave with you—forge on! You are on the path, the stars are over you, their light is flashing into your souls the slogan of the Federated Suns beyond the frontiers of your little warring worlds. Forge on!"

The Voice died out like the chiming of a great bell receding into immeasurable distance. The supercilious tones of the professor had yielded to the sweetness and the light of the Greater Mind whose instrument he had momentarily become. It was charged at the last with a golden resonance that seemed to echo down vast spaceless corridors beyond the furthermost outposts of time.

As the Voice faded out into a sacramental silence, the strangely assorted throng, moved by a common impulse, lowered their heads as though in prayer. The great globe pulsed and shimmered throughout its sentient depths like a sea of liquid jewels. Then the tentacle that grasped the professor drew him back toward the scintillating nucleus. Simultaneously another arm reached out and grasped Bill Jones, who, during the strange lecture, had ceased his wooden soldier marching and had stood stiffly at attention.

The bodies of both men within the nucleus were encircled once more by the single current. From it again put forth the tentacles, cupping their heads, but the smokelike essence flowed back to them this time, and with it flowed a tiny threadlike stream of violet light. Then came the heaving motion when the shimmering currents caught the two men and tossed them forth unharmed but visibly dowered with the radiance of more abundant life. Their faces were positively glowing and their eyes were illuminated by a light that was surely not of earth.

Then, before the very eyes of the marveling people, the great globe began to dwindle. The jeweled lights intensified, concentrated, merged, until at last remained only a single spot no larger than a pin-head, but whose radiance was, notwithstanding, searing, excruciating. Then the spot leaped up—up into the heavens, whirling, dipping and circling as in a gesture of farewell, and finally soaring into invisibility with the blinding speed of light.

The whole wildly improbable occurrence might have been dismissed as a queer case of mass delusion, for such cases are not unknown to history, had it not been followed by a convincing aftermath.

The culmination of a series of startling coincidences, both ridiculous and tragic, at last brought men face to face with an incontestable fact: namely, that Bill Jones had emerged from his fiery baptism endowed with the thought-expressing facilities of Professor Ralston, while the professor was forced to struggle along with the meager educational appliances of Bill Jones!

In this ironic manner the Space-Wanderer had left unquestionable proof of his visit by rendering a tribute to "innate intelligence" and playing a Jovian Jest upon an educated fool—a neat transposition.

A Columbus from a vaster, kindlier universe had paused for a moment to learn the story of our pigmy system. He had brought us a message from the outermost citadels of life and had flashed out again on his aeonic voyage from everlasting unto everlasting.

[er-cryptid]

Protoplast

-- the living part of a cell

-- includes the plasma membrane

-- does not include the cell wall

.

Patreon

[leedsomics]

Single-cell proteomics of Arabidopsis leaf mesophyll identifies drought stress-related proteins

The application of single-cell omics tools to biological systems can provide unique insights into diverse cellular populations and their heterogeneous responses to internal and external perturbations. Thus far, most single-cell studies in plant systems have been limited to RNA-sequencing approaches, which only provide indirect readouts of cellular functions. Here, we present a single-cell proteomics workflow for plant cells that integrates tape-sandwich protoplasting, piezoelectric cell sorting, nanoPOTS sample preparation, and FAIMS-based MS data acquisition method for label-free single-cell proteomics analysis of Arabidopsis leaf mesophyll cells. From a single leaf protoplast, over 3,000 proteins were quantified with high precision. The workflow is demonstrated to identify stress associated changes in protein abundance by analyzing >80 protoplasts from well-watered and water-deficit stressed plants. Additionally, we describe a new approach for constructing covarying protein networks at the single-cell level and demonstrate how single-cell protein covariation analysis can reveal previously unrecognized protein functions while also capturing stress-induced changes in protein-protein dynamics. http://dlvr.it/T98xQl

[spookysaladchaos]

Global Top 5 Companies Accounted for 77% of total Electroporators market (QYResearch, 2021)

Electroporation or electroosmosis is a microbiological technique in which an electric field is applied to a cell to increase the permeability of the cell membrane, allowing the introduction of chemicals, drugs or DNA into the cell. In microbiology, electroporation processes typically transform bacteria, yeast or plant protoplasts by introducing new coding DNA.

This report focuses on electroporators, consumable and reagent market.

According to the new market research report “Global Electroporators Market Report 2023-2029”, published by QYResearch, the global Electroporators market size is projected to reach USD 0.48 billion by 2029, at a CAGR of 6.6% during the forecast period.

Figure.   Global Electroporators Market Size (US$ Million), 2018-2029

Figure.   Global Electroporators Top 10 Players Ranking and Market Share（Based on data of 2021, Continually updated）

The global key manufacturers of Electroporators include Thermo Fisher Scientific, Lonza, Bio-Rad, Eppendorf, AngioDynamics, MaxCyte, Harvard Bioscience, Inc, Mirus, Nepa Gene Co., Ltd, BEX CO.LTD, etc. In 2021, the global top five players had a share approximately 77.0% in terms of revenue.

About QYResearch

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QYResearch is a world-renowned large-scale consulting company. The industry covers various high-tech industry chain market segments, spanning the semiconductor industry chain (semiconductor equipment and parts, semiconductor materials, ICs, Foundry, packaging and testing, discrete devices, sensors, optoelectronic devices), photovoltaic industry chain (equipment, cells, modules, auxiliary material brackets, inverters, power station terminals), new energy automobile industry chain (batteries and materials, auto parts, batteries, motors, electronic control, automotive semiconductors, etc.), communication industry chain (communication system equipment, terminal equipment, electronic components, RF front-end, optical modules, 4G/5G/6G, broadband, IoT, digital economy, AI), advanced materials industry Chain (metal materials, polymer materials, ceramic materials, nano materials, etc.), machinery manufacturing industry chain (CNC machine tools, construction machinery, electrical machinery, 3C automation, industrial robots, lasers, industrial control, drones), food, beverages and pharmaceuticals, medical equipment, agriculture, etc.

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