#cellular aging

[medicomunicare]

An UBIquitous Parkin to take a NAP iSINT BAD while OPTN' for cargo hold: but TANK-binding will lead to a mit-o-loss

There are several pathways to induce mitophagy, or the self-degradation of irreversibly damaged cellular mitochondria. The most well-characterized of these depends on proteins called PINK1 and Parkin, two proteins actively involved in the abnormalities of brain cells affected by Parkinson’s disease. Two other proteins, TFEB and HKDC1, play a key role in the maintenance of both mitochondria and…

[scienceswitch]

The Science of Aging: What Happens to Our Bodies As We Get Older?

Aging is a natural and inevitable journey that all living organisms experience. As we progress through the different stages of life, our bodies undergo profound physiological changes. These transformations reflect the complex biological choreography that shapes human development and maturation. Aging is defined as the accumulation of changes over time associated with or responsible for the…

[jenks378]

Methylene Blue : Potential Benefits For Anti-Aging, Brain Health And Mor...

[mindblowingscience]

Genetically engineered mitochondria can convert light energy into chemical energy that cells can use, ultimately extending the life of the roundworm C. elegans, a new study shows.

While the prospect of sunlight-charged cells in humans is more science fiction than science, the research, which takes a page from the field of renewable energy, sheds light on important mechanisms in the aging process.

“We know that mitochondrial dysfunction is a consequence of aging,” says Andrew Wojtovich, associate professor of anesthesiology and perioperative medicine and pharmacology and physiology at the University of Rochester Medical Center, as well as senior author of the study in Nature Aging.

“This study found that simply boosting metabolism using light-powered mitochondria gave laboratory worms longer, healthier lives. These findings and new research tools will enable us to further study mitochondria and identify new ways to treat age-related diseases and age healthier.”

Continue Reading

[landwriter]

🍋 What’s your favorite spicier trope to write?

fruit fic asks!

DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK DIRTY TALK

[deva-arts]

Vincent has dark roots and white hair. Does he dye it?

Nope. His hair just fades in that color past a certain length. If he were to let it all grow out instead of shaving the sides all the time, it would all look white and spiky.

(Similarly, if he buzzed it all off, it would be black.)

[fish-bowl-2]

Thoughts on Ed Edd n Eddy being a Millennium era (1999 - 2000) period piece?

[cleverthylacine]

be pro-ageing but not pro-biological damage.  It’s fine to admire signs of a life well lived but just like I can admire scars without wanting to have events in my life that lead to them, I admire healthy aging but I’m not a fan of collagen loss or bone loss or sun damage.  I do many things to keep myself looking younger than my age and am pleased when people are surprised by it, but that is largely because I do not like having uncomfortable skin or feeling like a single fall will kill me.

There is much to be admired in the wisdom gained from living a long life; if you have not died by the time you’re 70 or 80 or 90 or 100+, you must have done something right and you must have some survival skills.

But you can admire the life experience and not the fragility.

be pro-aging but wear sun screen. sun protection is not beauty industry propaganda it will save you. wear it. or else.

[bitcoinversus]

BioTechnology: Washington Professor Says Future Humans May Live Up to 500 Years

Pedro Domingos, a professor emeritus at the University of Washington and author of The Master Algorithm and 2040, envisions a future where humans may achieve unprecedented lifespans, potentially living up to 500 years. Future humans will have much bigger brains, live to 500 and be children for the first 50 years.— Pedro Domingos (@pmddomingos) October 20, 2024 He suggests that such longevity…

[kevinbutlerweb]

Increase Your Power and Enhance Your Wellness with NAD Infusion

Are you feeling drained pipes and lacking the power to tackle your daily jobs? Have you been looking for a natural way to boost your wellness and general health? Look no more than NAD infusion therapy-- an advanced treatment that can assist increase your power levels and revitalize your body from within.NAD, or Nicotinamide Adenine Dinucleotide, is a coenzyme discovered in every cell of your body that plays an essential function in power manufacturing. As we age, our NAD levels naturally decline, causing reduced power levels and general vitality. NAD mixture therapy functions by directly providing this necessary coenzyme right into your bloodstream, bypassing the digestion system for maximum absorption and effectiveness.One of the key benefits of NAD mixture treatment is its capability to considerably raise energy levels. By renewing your body's NAD shops, you can experience a visible boost in stamina, emphasis, and psychological clearness. Bid farewell to afternoon downturns and hey there to continual energy throughout the day.But the benefits of NAD infusion therapy extend much beyond just enhanced energy levels. This innovative treatment has actually been shown to boost general health and wellness and health in various methods. From sustaining mobile repair work and regeneration to boosting cognitive function and mood stability, NAD mixture therapy supplies a comprehensive method to enhancing your wellness from the within out.Unlike traditional energy-boosting techniques like caffeine or stimulants that offer short-term solutions with prospective negative effects, NAD infusion treatment takes an alternative strategy to wellness. By attending to the source of reduced power levels-- decreasing NAD degrees-- this treatment sustains your body's natural processes for lasting outcomes with no harmful ingredients or chemicals.If you prepare to experience the transformative power of NAD infusion therapy on your own, getting going is simpler than you think. Just arrange an assessment with a certified doctor that focuses on this cutting-edge treatment. During your preliminary visit, you'll discuss your wellness objectives and medical history to identify if NAD mixture treatment is best for you.Once accepted for therapy, you can look forward to kicking back sessions where NAD is provided intravenously under clinical supervision. Numerous people report feeling an instant sense of restoration after their very first session, with advancing advantages experienced over subsequent treatments.In conclusion, if you're seeking to improve your power levels, improve your well-being, and take an aggressive approach to your health, take into consideration integrating NAD infusion therapy into your wellness regimen. With its tested advantages for energy production, total health enhancement, ...

nad infusion

[medicomunicare]

Invecchiamento del corpo umano: cambiamenti drammatici a due specifiche età, secondo uno studio

Introduzione L’invecchiamento è un processo biologico complesso che interessa ogni aspetto della funzione corporea. Sebbene sia spesso percepito come un processo graduale, recenti studi suggeriscono che il corpo umano potrebbe sperimentare cambiamenti particolarmente drastici in momenti specifici della vita. Un recente studio ha identificato due età chiave in cui si verificano cambiamenti…

[healthyhabitjournal]

🌟 Dreaming of youth eternal? Discover the natural secret within our DNA! 🧬 Telomere Lengthening is changing the game in anti-aging, offering a path to longevity and vibrant health without the need for expensive treatments. Dive into natural, effective strategies to protect your DNA and unlock a life filled with vitality. Say yes to a healthier, younger you today! #TelomereLengthening #NaturalYouth #HealthyHabitJournal

[kyrianclawraith]

Ah, but given his dating/hookup history I'd say that the important word in that sentence is "today". He's probably going to take a bit to decompress and get used to the situation, but then come back swinging his shot right back at them. I mean John's kinda doing the same thing as Danny here, since he's an old man himself. (He's just not stopping his healing factor from producing the needed telomerase to stay in his prime like Danny is.)

Danny is the Crazy Old Man™️ of Gotham

So, the events of Danny Phantom happened decades ago

Like, Phantom Planet was one of the first instances of Superheroes in HISTORY. Early 1900's, just the Fentons were Insanely Ahead of their Time!

Danny is still a Halfa, but has allowed himself to grow old and live his best life before fully dying so he can accept his Throne in the Infinite Realms. He decides to experience Life in the fullest way possible, partying, drinking, making long lasting friendships that shape the lives of everybody he meets, all that!

Eventually, Danny's Party Life leads him to Gotham. And this place is just amazing!

It has all the comforts of Home, with so much more! He can Party! He can Fight! He can do anything he wants and nobody bats an eye, because a crazy old man getting into a fistfight in the middle of the road is just another Tuesday for Gotham!

He decides to spend the rest of his Mortal Life there. And this is still Early On in the DC Timeline, like, Batman Year 1 is happening Right Now.

He hangs around, befriends the local Homeless Population, and mostly just has the time of his Life! And he takes up the stereotypical Homeless Old Man look because why fight it? That's literally what he's going for!

He also unintentionally sets up a bunch of future events

He teaches Kid!Jason on his to steal Tires as repayment for driving off some muggers with a Baseball Bat (honestly he was looking forward to being mugged, it's a new experience after all)

He pulls Kid!Tim into an Alley after Tim gets caught out at night and gets chased by some Punks. He hides Tim behind a Dumpster and tricks the Punks into mugging him instead (Yay! He finally got mugged!)

He becomes kind of well known as the Old Man who wants to experience everything before he dies. He says as much too, not like he really has a reason to hide it. He just tells people "I want to live my life to the fullest, it don't matter if I live 10 more years or 10 more minutes, I'm gonna experience every second of it!"

He once walked into a Cloud of Fear Gas to see what it was like. Later he said it was a 6/10. "Not the worst thing I've had injected into my body!" He says with no Context.

He traded places with a Hostage during an active Crime Scene because he wanted to know what it's like.

He was once dared to take Batmans Utility Belt by another Homeless Guy as a joke, so he walked up to Batman later that night in full view of everybody else and just asked for his Belt. He gives up after a few minutes, and one guy asked "Why not fight him for it? It's an experience after all.". Danny replys "Nah, I've fought Vigilantes before. It was fun though, gotta say!"

...

This got away from me, but all this to say: Imagine the Bat Families Reaction when they find out "Crazy Old Danny" is PHANTOM. You know, THE FIRST SUPERHERO!

I imagine Constantine is having a stroll though Gotham after finishing up some business with Bruce, and just bumps into a homeless guy by accident.

Later that night:

Batman: Constantine, Why are you calling? Is it to do with the-

Constantine: Why the fuck is there a Homeless God in your City?

Batman: Wait wha-

...

Or imagine they know before Constantine meets him, and it goes instead like this

Constantine: Why the fuck is there a Homeless God in your City?!

Batman: You mean Old Man Danny? He's just a homeless guy? What do you mean?

Constantine: I swear on what's left of my Soul, that is a God.

Batman, a little shit: I don't think so, I would know (fully knows)

[reasonsforhope]

A new treatment combining ReCET and semaglutide could eliminate the need for insulin in type 2 diabetes, with 86% of participants in a study no longer requiring insulin therapy. The treatment was safe and well-tolerated, and further trials are planned to confirm these results.

Groundbreaking research presented at UEG Week 2024 introduces a promising new treatment approach for type 2 diabetes (T2D) that has the potential to greatly reduce or even eliminate the need for insulin therapy.

This innovative approach, which combines a novel procedure known as ReCET (Re-Cellularization via Electroporation Therapy) with semaglutide, resulted in the elimination of insulin therapy for 86% of patients.

Globally, T2D affects 422 million people... While insulin therapy is commonly used to manage blood sugar levels in T2D patients, it can result in side effects... and further complicate diabetes management. [Note: Also very importantly it's fucking bankrupting people who need it!!] A need therefore exists for alternative treatment strategies.

Study Design and Outcomes

The first-in-human study included 14 participants aged 28 to 75 years, with body mass indices ranging from 24 to 40 kg/m². Each participant underwent the ReCET procedure under deep sedation, a treatment intended to improve the body’s sensitivity to its own insulin. Following the procedure, participants adhered to a two-week isocaloric liquid diet, after which semaglutide was gradually titrated up to 1mg/week.

Remarkably, at the 6- and 12-month follow-up, 86% of participants (12 out of 14) no longer required insulin therapy, and this success continued through the 24-month follow-up. In these cases, all patients maintained glycaemic control, with HbA1c levels remaining below 7.5%.

Tolerability and Safety

The maximum dose of semaglutide was well-tolerated by 93% of participants, one individual could not increase to the maximum dose due to nausea. All patients successfully completed the ReCET procedure, and no serious adverse effects were reported.

Dr Celine Busch, lead author of the study, commented, “These findings are very encouraging, suggesting that ReCET is a safe and feasible procedure that, when combined with semaglutide, can effectively eliminate the need for insulin therapy.”

“Unlike drug therapy, which requires daily medication adherence, ReCET is compliance-free [meaning: you don't have to take it every day], addressing the critical issue of ongoing patient adherence in the management of T2D. In addition, the treatment is disease-modifying: it improves the patient’s sensitivity to their own (endogenous) insulin, tackling the root cause of the disease, as opposed to currently available drug therapies, that are at best disease-controlling.”

Looking ahead, the researchers plan to conduct larger randomized controlled trials to further validate these findings. Dr. Busch added, “We are currently conducting the EMINENT-2 trial with the same inclusion and exclusion criteria and administration of semaglutide, but with either a sham procedure or ReCET. This study will also include mechanistic assessments to evaluate the underlying mechanism of ReCET.”

-via SciTechDaily, October 17, 2024

--

Note: If it works even half as well as suggested, this could free so many people from the burden of the ongoing ridiculous cost of insulin. Pharma companies that make insulin can go choke (hopefully).

[beautyarchive]

Kim Basinger in Cellular (2004).

[jcmarchi]

A new way to see the activity inside a living cell

New Post has been published on https://thedigitalinsider.com/a-new-way-to-see-the-activity-inside-a-living-cell/

A new way to see the activity inside a living cell

Living cells are bombarded with many kinds of incoming molecular signal that influence their behavior. Being able to measure those signals and how cells respond to them through downstream molecular signaling networks could help scientists learn much more about how cells work, including what happens as they age or become diseased.

Right now, this kind of comprehensive study is not possible because current techniques for imaging cells are limited to just a handful of different molecule types within a cell at one time. However, MIT researchers have developed an alternative method that allows them to observe up to seven different molecules at a time, and potentially even more than that.

“There are many examples in biology where an event triggers a long downstream cascade of events, which then causes a specific cellular function,” says Edward Boyden, the Y. Eva Tan Professor in Neurotechnology. “How does that occur? It’s arguably one of the fundamental problems of biology, and so we wondered, could you simply watch it happen?”

The new approach makes use of green or red fluorescent molecules that flicker on and off at different rates. By imaging a cell over several seconds, minutes, or hours, and then extracting each of the fluorescent signals using a computational algorithm, the amount of each target protein can be tracked as it changes over time.

Boyden, who is also a professor of biological engineering and of brain and cognitive sciences at MIT, a Howard Hughes Medical Institute investigator, and a member of MIT’s McGovern Institute for Brain Research and Koch Institute for Integrative Cancer Research, as well as the co-director of the K. Lisa Yang Center for Bionics, is the senior author of the study, which appears today in Cell. MIT postdoc Yong Qian is the lead author of the paper.

Fluorescent signals

Labeling molecules inside cells with fluorescent proteins has allowed researchers to learn a great deal about the functions of many cellular molecules. This type of study is often done with green fluorescent protein (GFP), which was first deployed for imaging in the 1990s. Since then, several fluorescent proteins that glow in other colors have been developed for experimental use.

However, a typical light microscope can only distinguish two or three of these colors, allowing researchers only a tiny glimpse of the overall activity that is happening inside a cell. If they could track a greater number of labeled molecules, researchers could measure a brain cell’s response to different neurotransmitters during learning, for example, or investigate the signals that prompt a cancer cell to metastasize.

“Ideally, you would be able to watch the signals in a cell as they fluctuate in real time, and then you could understand how they relate to each other. That would tell you how the cell computes,” Boyden says. “The problem is that you can’t watch very many things at the same time.”

In 2020, Boyden’s lab developed a way to simultaneously image up to five different molecules within a cell, by targeting glowing reporters to distinct locations inside the cell. This approach, known as “spatial multiplexing,” allows researchers to distinguish signals for different molecules even though they may all be fluorescing the same color.

In the new study, the researchers took a different approach: Instead of distinguishing signals based on their physical location, they created fluorescent signals that vary over time. The technique relies on “switchable fluorophores” — fluorescent proteins that turn on and off at a specific rate. For this study, Boyden and his group members identified four green switchable fluorophores, and then engineered two more, all of which turn on and off at different rates. They also identified two red fluorescent proteins that switch at different rates, and engineered one additional red fluorophore.

Each of these switchable fluorophores can be used to label a different type of molecule within a living cell, such an enzyme, signaling protein, or part of the cell cytoskeleton. After imaging the cell for several minutes, hours, or even days, the researchers use a computational algorithm to pick out the specific signal from each fluorophore, analogous to how the human ear can pick out different frequencies of sound.

“In a symphony orchestra, you have high-pitched instruments, like the flute, and low-pitched instruments, like a tuba. And in the middle are instruments like the trumpet. They all have different sounds, and our ear sorts them out,” Boyden says.

The mathematical technique that the researchers used to analyze the fluorophore signals is known as linear unmixing. This method can extract different fluorophore signals, similar to how the human ear uses a mathematical model known as a Fourier transform to extract different pitches from a piece of music.

Once this analysis is complete, the researchers can see when and where each of the fluorescently labeled molecules were found in the cell during the entire imaging period. The imaging itself can be done with a simple light microscope, with no specialized equipment required.

Biological phenomena

In this study, the researchers demonstrated their approach by labeling six different molecules involved in the cell division cycle, in mammalian cells. This allowed them to identify patterns in how the levels of enzymes called cyclin-dependent kinases change as a cell progresses through the cell cycle.

The researchers also showed that they could label other types of kinases, which are involved in nearly every aspect of cell signaling, as well as cell structures and organelles such as the cytoskeleton and mitochondria. In addition to their experiments using mammalian cells grown in a lab dish, the researchers showed that this technique could work in the brains of zebrafish larvae.

This method could be useful for observing how cells respond to any kind of input, such as nutrients, immune system factors, hormones, or neurotransmitters, according to the researchers. It could also be used to study how cells respond to changes in gene expression or genetic mutations. All of these factors play important roles in biological phenomena such as growth, aging, cancer, neurodegeneration, and memory formation.

“You could consider all of these phenomena to represent a general class of biological problem, where some short-term event — like eating a nutrient, learning something, or getting an infection — generates a long-term change,” Boyden says.

In addition to pursuing those types of studies, Boyden’s lab is also working on expanding the repertoire of switchable fluorophores so that they can study even more signals within a cell. They also hope to adapt the system so that it could be used in mouse models.

The research was funded by an Alana Fellowship, K. Lisa Yang, John Doerr, Jed McCaleb, James Fickel, Ashar Aziz, the K. Lisa Yang and Hock E. Tan Center for Molecular Therapeutics at MIT, the Howard Hughes Medical Institute, and the National Institutes of Health.