#but they're rarely induced by fake claiming

[cccat-in-a-meat-sack]

NPD + DID culture is actively hoping we get posted to r/syscringe or r/fdc because tHE AMOUNT OF SUPPLY THAT WOULD BRING OH MY GOD

[catboybiologist]

Hey I feel like I remember someone using crispr (or other gene editing method) to successfully edit her own genome and since I saw the article on tumblr and I think I remember her doing it for trans reasons it feels like something you'd know about. Google isn't helping, do you know who im talking about?

Yup I remember that.

Tl;Dr: I don't buy this instance of it, but there's some nuggets of theory that she built upon that are reasonable.

I'm gonna try to keep this brief and simple.

Here was the basic theory, extremely oversimplified: normal cell development follows a kind of "tree". A stem cell can become a couple different kinds of other stem cells that are more specific, and onwards, until they become "fully differentiated" cell types. These are the cells we know and love: nerve, skin, muscle, etc.

Stem cell research is so valuable because of this- "replacement" tissue can be generated for any other kind of cell with stem cells.

But in extremely rare cases, a process called transdifferentiation can occur. Essentially, a fully differentiated cell type becomes a different cell type without reverting to a stem cell state at all. Imagine a bit of heart tissue becoming an intestine.

It's an extremely poorly understood process. Like, EXTREMELY poorly understood, and the basic dynamics of how it works are a subject of active research. However, it's generally understood that in some organisms, under some extreme external circumstances, in certain cell types, it can be induced by adding in or blocking the right signal.

Based on one study that did a trial on natal male mice, there is a gene that, when knocked down, MIGHT cause transdifferentiation of testes cells into ovarian tissue, which would then secrete estrogens.

CRISPR is used to target a small segment of DNA and cleanly "cut" it out of the genome.

Now. What did this woman actually do?

Well, we don't have much to go on. It was a single thread, less than a paragraph in total, from a "bio hackers" forum. The only data provided was a text document of estrogen levels, one time point before and one time point after, and a diagram of the insert vector that would target the gene mentioned before. What she's claiming she did is inject this vector into her testes, and that the resulting estrogen level afterwards is from her being off HRT, and therefore is a result of ovarian tissue producing it.

But there's SO many confounding factors here, and SO little to go on. Self-professed "bio hackers" are kinda notorious for not having great ideas of what they're doing- not in a dangerous way, more in a "wasting time and money on things that don't work" way. And even for them, there's almost nothing to go on here.

Am I saying the entire thing is fake? Not necessarily, I don't like to jump to "people are lying". However, I am saying that there are too many confounding factors that could have easily kept E high for a long time to question Contrary to what popular media would have you believe, CRISPR is extremely fickle, and not well optimized for humans. I'm also saying that, without additional time points or verification of data, those hormones levels could just be taking a longer time to change.

In fact, I literally consider it more likely that transfemme HRT for extended periods of time might be able to cause transdifferentiation in extremely small amounts on its own. It's been possibly documented going the other direction:

I say possibly because there are other potential explanations that haven't been investigated yet, and it's never been studied in transfemmes. Either way, is it enough to endogenously produce hormones at cis female levels? Maybe, after a decade or so. But I want to see a lot more investigation into it.

Generously, I want to say that long term HRT did cause a lasting change- either the pituitary suppression of testosterone was so prolonged that it took a long time to rebound, or there actually is some transdifferentiation happening as a result of previous HRT itself. I don't think there's a world where the CRISPR actually worked.

Now. Is CRISPR to edit the genome to cause transdifferentiation of testes into ovarian tissue, that would be just enough of an ovary to produce estradiol, theoretically possible? Probably yes, actually! But did someone do it with no documentation, minimal funding, and then disappear afterwards? Not really buying it.