#NoSLES

[thebadchoicemachine]

HOW THE FUCK DO PEOPLE LIGHT CANDLES LIGHTERS ARE SO SO SCARY???

Zippo/gas station lighters + candle with a low wick = ow my fingers

[nosferatini]

[🎙️Podfic] Geminids - A “Teach Me” Side Story

By @nosferatini

The lovely NooRose93 and incredibly talented @gahellhimself-blog allowed me to make a podfic of this beautiful story of brotherly love!

Listen to the Geminids Podfic! 🎧

Featuring little Nosling as the voice of baby Crowley 👶🏻

(Note: No babies went underfed or uncuddleld in the making of this podfic!)

Geminids is a side story for GaHell's wonderful "Teach Me" comic, written by NooRose93. Read Geminids - A Teach Me side story on AO3!

Please support GaHell on his Patreon and Instagram!

**Summary:**

Loki and Crowley are there for each other through thick and thin. This is a story of how they met and became brothers.

Special thanks to my beta listeners @kunigun and @outrageousring5655 ♥️ as well as the @goodomensafterdark and @whickberstreetwriters communities for all the love and support in making this podfic.

[swirly-lemonade]

Fine fine fine I’ll drink some water but also drink coffee afterwards-

Should I drink another cup of coffee?

[wildbeautifuldamned]

Christopher Radko A Gift of Nature Santa Christmas Ornament - 7.5H. NOSLE ebay Buy Collectibles Inc

[roseblack2222]

We're trapped in an abandoned hospital -- Written by @RoseBlack2222 Nosl...

Who likes horror narrations? If you do, check out Lady Spookaria who has posted a narration of my abandoned hospital series. If you enjoy this reading, give us both money! Sharing our work helps too.

[hardynwa]

Akwa Ibom: Community protests, blocks oil firm over alleged environmental degradation

Ukpana Community in Onna Local Government Area of Akwa Ibom State, on Wednesday, blocked an oil firm, Natural Oilfield Services Limited, NOSL, operating in their area with the claims that its activities have destroyed their environment, economic trees, water bodies and traditional shrines. They mentioned the creeks, streams and swamps bulldozed and sand filled to include Idim Mkpighe, Idim Uton, Etuk Enyang and a host of others while Ancestral shrines destroyed include Nkuku, Ikot Inwang, Ikpaisong Ubop Owo and others. NOSL, which has a joint management team with the Nigeria National Petroleum Exploration and Production Limited, is an operator of OML 13 in Oniong Clan, Onna LGA and engages in oil and gas appraisal as well as well drilling. The protesters, who were men and women, nursing mothers and youths, placed a traditional injunction, demanding the stoppage of the operation of the company until their demands were met. In their placards, the protesters expressed anger over the alleged nonchalant attitudes of the management of the company, which they alleged have continued to pay deaf ears to their plights. Some of the placards read ” NOSL has trespassed into our land without consulting us” ” You have destroyed our means of livelihood” “We have been made to suffer unduly in our own land” among others. The village head of Ukpana, Chief Ranzi Bassey, who spoke with journalists after the protest lamented the massive destruction of their crops and economic trees, destruction of buildings and structures, noise pollution and others which warranted the village council to notify NOSL through their community Attorney–AIbom Oil Producing Community Development Network, AKIPCON, to no avail. He said, “Ukpana people have been taken for granted since November 2019 when NOSL forcefully entered and cleared larger hectares of their farmlands, destroyed our houses, economic trees, food crops, building and structures, ancestral shrines /deities, fishing framing, streams, swamps, earth graves and tombstones with attendants health and human rights consequences”. Chief Bassey said a lot of creeks, streams and swamps have been seriously bulldozed and sand-filled without the consent of villagers. Also speaking, one of the protesters who identified himself as Joseph Israel lamented the economic hardship and insecurity posed on the youth of the community who have no alternative means of livelihood following the destruction inflicted on them by NOSL. According to him ” the community has suffered untold hardship, unwanted trespass and destruction through oil exploration activities going on in the area. The massive destruction occurred during the construction of an access road to the appraisal well drilling site at the company’s operational base.” Read the full article

[sbgridconsortium]

Radical Reactions - Yvain Nicolet - Institut de Biologie Structurale

Sharing some of our #SBGrid member tales from the last year. This one from January 2023.

The periodic table may be an icon of chemistry, but a cluster of elements at its center gives biology some essential pop and sparkle. Yvain Nicolet at the Institut de Biologie Structurale (IBS) in Grenoble, France, wants to learn exactly how those transition metals in the middle of the table put more pizzazz in proteins.

About one-third of proteins contain transition metals, such as cobalt, copper, molybdenum, or iron. The Nicolet lab mainly studies metalloproteins that contain iron-sulfur clusters but also scrutinizes enzymes that make these clusters. "Transition metals are often key components of the proteins in terms of function," Nicolet says. "They are only few atoms in a structure and sit in specific sites, but they enable specific activities, notably in enzymes, but not only in enzymes." Metals give proteins some superpowers in the form of new chemical properties and functions not otherwise possible with combinations of the 22 amino acids.

"They enhance the reactivity of the proteins by making many strange active sites that are very interesting," he says. These reactions can be blazingly fast with complex chemistry. And they are technically challenging to study.

The lab investigates enzymes that use metals, as well as proteins that build the metal cofactors and insert them into the enzymes. "We try to trigger the reaction in a crystal and to trap the different intermediates during the reaction to characterize by structural biology," Nicolet says.

Many metalloproteins evolved in a time before oxygen and can only make their razzle-dazzle moves in an anaerobic space. To prevent oxygen from destroying their molecules, experiments in the Nicolet lab take place in a series of six large anaerobic chambers filled with nitrogen.

The researchers reach in through gloves built into the airtight boxes to work with samples and equipment. There, cells are grown and broken up. The cell extracts are cleared and proteins separated by chromatography. Crystallization robots in one glove box screen for optimum conditions that researchers can manually reproduce in another box and freeze in liquid nitrogen. From there, the synchrotron is only 500 meters away.

"We determine 3D structures at moderate to high resolution," Nicolet says. "At first, we aim at determining where the metals are in a given protein, and how they interact with their environment (the protein matrix). We also aim at determining the structure of the metallocofactors or metal binding sites in terms of ligands and geometry.

"Then in a second step, we aim at understanding how these metals confer a specific activity to the protein," he continues. "For instance, in an enzyme, we would get intermediate states with the metal in a different coordinate and/or redox state to better understand what is going on during the reaction."

One milestone of the lab's work arose from successfully trapping an unexpected radical intermediate of NosL, a radical S-adenosyl-L-methionine (SAM) enzyme, in the process of generating a peptide with potent activity against gram-positive bacterial pathogens (Science, 2016).

Crystallography provided the position of the atoms, and for this paper they also used a spectroscopy technique called electron paramagnetic resonance (EPR), which gives the position of unpaired electrons near atoms.

Two months later, the group reported results from a study in which they carried out radical based chemistry catalyzed by the radical SAM enzyme HydE from beginning to end in a crystal (Nature Chemistry, 2016).

The image shows substrate processing and product transfer within HydE cavity during the FeFe-hydrogenase active site assembly process. Credit: Y.Nicolet

More recently, Nicolet's team characterized intermediates in the assembly of the nitrogenase, a key player in the global nitrogen cycle. The enzymatic complex nitrogenase catalyzes the reduction of nitrogen gas N2 to ammonia NH3, transforming nitrogen and returning it to plants. Specifically, the paper reported the X-ray structure of NifB protein from a nitrogen-fixing bacteria, one of a dozen proteins required to assemble the nitrogenase active site (Journal of the American Chemical Society, May 2020).

"For nitrogenase, there is a dedicated machinery with very, very challenging chemistry involved, and this is what we are attacking over the few next years," he says.

Nicolet was born and raised in Grenoble, France, where he now lives and works. Growing up in the city, he became an accomplished cello player with ambitions of becoming a professional musician. He started college in Grenoble. When a music school in Paris turned down his application, he chose to pursue science at what is now the University of Strasbourg. In Strasbourg, all the science classes emphasized the three-dimensional structures of molecules, whether it was enzymology, DNA replication, or proteins. He completed his undergraduate degree and stayed for graduate training in chemistry, biology, crystallography and nuclear magnetic resonance (NMR).

In a dinner discussion, a friend noted his interest in inorganic chemistry and suggested a structural biology lab in Grenoble working on metalloproteins. He moved back to complete his PhD at what is now the Université Grenoble Alpes. Nicolet worked on the structure of the [FeFe]-hydrogenase (an enzyme that makes or use molecular hydrogen), basic science with a potential application in producing alternative fuel.

From there, he joined the Drennan Lab at the Massachusetts Institute of Technology. In Cambridge, he discovered radical-based chemistry working on this newfound Radical SAM Proteins superfamily, now known for their ability to synthesize many vitamins, cofactors, or antibiotics.

He moved back to Grenoble for a one-year postdoctoral fellowship with the European Synchrotron Radiation Facility. In 2004, he joined IBS as a scientist and became a group leader there in 2016.

The Nicolet lab often dives into the molecular mechanisms of phenomenon discovered by other groups. "In my lab, we are not at the beginning of the discovery, and we are probably not at the end of the process," he says. "We focus on the molecular mechanisms inside the enzymes."

In a new challenge, the lab has set up a system to use cryo-electron microscopy to examine the assembly dynamics and reactions at room temperature without oxygen. Nicolet is interested in the basic science of radical chemistry, but he also hopes to be able to harness a radical enzyme and modify its catalytic activity to produce a molecule through directed evolution, such as a better antibiotic.

Nicolet also enjoys taking a bigger picture view of the nitrogen cycle, thanks to his garden. He came late to the activity and confesses that he is not good at growing vegetables. "You can observe the nature or the interaction between the different organisms that live together from bacteria to insects and plants," he says. "For me, it's the best way to contemplate what Darwin described as natural selection. And evolution. I'm not very good at producing vegetables, but I really enjoy seeing how all these organisms compete together or help each other as well.

-Carol Cruzan Morton

[rausule]

klossie

ciuffo

nek

stam van die stert

fusto della coda

kroep

groppa

punt van kruis

punta della groppa

terug

dorso

-verwelk

garrese

helmteken

cresta

maanhare

criniera

-nek

collo

kaak-

mandibola

neusgat

narice

brom hy.

borbozza

——

niere

reni

heuppunt

punta dell'anca

keel

gola

kwartiere

quarti

jugulêre stort

doccia giugulare

buigspier

muscolo flessore

gluteale

glutel

van die nek

del collo

flank voorhuid

fianco prepuzio

skouer

spalla

verstik

grassella

bors

petto

skouer punt

punto della spalla

elmboog-

gomito

arm

braccio

buik

addome

been

gamba

punt van die hak

punta del garretto

knie

ginocchio

kastaiingbruin.

castagna

hak

garretto

fleksor tendon

tendine flessore

shin-

stinco

pastoraal

pastorale

fetlock

nodello

aansporing

sperone

hoef

zoccolo

hakke

talloni.

floccum

occipitium

cauda caudam

croup

tip of caudam

retro

-withers

crista

iuba

-neck

maxilla-

nosl

murmurat.

--

renes

coxae tip

faucium

quarteria

jugularis imber

flexor flexor digitorum longus

gluteal

colli

lateri praeputii

humero

suffocare

pectus

humero punctus

cubitus-

arm

abdominis

leg

apicem gregem

genu

castaneis.

hock

flexor flexor digitorum longus

shin-

pastoralis

fetlock

calcar

ungula

calcibus

DIE KONFORMASIE VAN DIE PERD

Die eerste evaluering wat gemaak word van a

perd is die een waaruit afgelei kan word

inligting wat uit waarneming kom

van sy eksterne konformasie. Hierdie

waarneming, wat die eerste fase van 'n pluspunt is

in-diepte studie, kan toelaat dat die

perdekenner om die tien-

aanleg van die betrokke vak a

volgens die eienskappe van sy verskillende dele van die liggaam en die verhoudings tussen hulle.

Sedert antieke tye, deur noukeurige waarneming van die perd en sy bewegings, het die mens probeer om die betekenis en gevolge van sy fisiese bouvorm te verstaan, wat bepaalde gesindheid kan beteken, om die beste gebruik daarvan te maak en te probeer om in die nageslag dié wat hy die gerieflikste geag het.

Perde wat vir verskillende werke gebruik word, het natuurlik verskillende eienskappe van vorm en proporsies, sodat wat in een ras as 'n bate beskou word, eerder as 'n gebrek in 'n ander beskou kan word: kort en robuuste ledemate met groot hoewe is ongetwyfeld 'n ernstige gebrek in.

[sequoiadawn]

Briogeo Superfoods Value Hair Pack! 💚

• Matcha + Apple Replenishing Superfood Shampoo 🍵

• Kale + Apple Replenishing Superfood Conditioner 🍏

This duo offers six-free hair care meaning: no harsh sulfates (SLS/SLES), no silicones, no phthalates, no parabens, no DEA, and no artificial dyes. They are also all gluten-free, cruelty-free, and vegan! 🌱

[taylorfantasyblonde]

#lakonikabio #lakonika #шампунь #бальзамкондиционер #shampoo #conditioner #nopeg #nosls #nosles #нетпарабенов #нетфеноксиэтанола #бальзам #кондиционер https://www.instagram.com/p/COhDJSLBGFw/?igshid=41yg0qu083vl

[madhuscooking]

Natural Dishwasher Liquid |Natural Floor Cleaner |NO SULFATES, CHEMICALS |Homemade Dishwasher Liquid

[fstopcollective]

Getting ready with Lesley at Haynes Village Hall Afternoon Tea event. Looks like it should be a great event! #gift #bubbles #bubblebath #bathbomb #soap #nosls #NoSLES #parabenfree #giftfair (at Haynes village hall) https://www.instagram.com/p/B7gHGaZHaNu/?igshid=1rozfu49cau6k

[newfashionsoapco]

For just $2.50 NATURAL GLYCERIN SOAP, MADE WITH ORGANIC CINNAMON ESSENTIAL OIL IN REPURPOSED GLASS BOTTLE WITH OR WITHOUT PUMP OR 3 OZ PLASTIC BOTTLE. VERY GENTLE ON YOUR HANDS. ANY SHIPPING OVERCHARGES WITH MULTIPLE ORDERS WILL BE REIMBURSED AFTER THE ORDER IS PLACED. I CAN FIT UP TO 8 BOTTLES (ANY 9OZ) IN MEDIUM FLAT RATE BOX FOR $ 14.50 COMMERCIAL SOAPS ARE MADE IN LARGE BATCHES TO KEEP THE COST AS LOW AS POSSIBLE. THE RESULTING PRODUCT ARE SOAPS WITH ADDED DETERGENTS [WHITCH ARE PETROLEUM BASED PRODUCTS LIKE GASOLINE AND KEROSENE], SURFACTANTS [CHEMICALS TO MAKE MORE SUDS] AND HAVE SYNTHETIC PRESERVATIVES AND COLORANTS AND PERFUMES. THE INGREDIENTS IN COMMERCIAL SOAPS TYPICALLY INCLUDE SODIUM LAUREL SULPHATE [CAUSES IRRITATION IN MANY INDIVIDUALS], PETROLEUM AND EVEN FORMALDEHYDE. OUR SKIN IS THE LARGEST ORGAN OF THE HUMAN BODY AND WILL ABSORB WHAT IS PUT ONTO THE BODY. EVERY SOAP EVER MADE NEEDS COMBINATION OF OILS/FATS AND LYE. It is made by a reaction (called saponification) between caustic soda (sodium hydroxide) or caustic potash (potassium hydroxide) with animal and/or vegetable fats (oils). As with all of our handcrafted products, soap is made in small batches by hand with only the purest ingredients, they do not contain any preservatives or fillers. All of the natural glycerin is retained during the soap making process so our soap cleans without stripping away natural oils. It is a gentle, luxurious soap with a creamy lather. NO SLS, NO SYNTHETIC COLOR, NO PARABENS, NO DETERGENTS, NO SYNTHETIC FRAGRANCE. Due to the handmade nature of our products, color, design,weight and shape may vary slightly. If you experience any irritation please discontinue use. PLEASE CONTACT ME WITH ANY QUESTIONS. Essential Oils are highly concentrated and potent oils extracted from plants, leaves, flowers, roots, buds, twigs, rhizomes, heartwood, bark, resin, seeds and fruits. Essential Oils are found in special secretory glands or cells within p...

[beast-feast]

Drawing blind

Starring:

Failed Eldritch

Hollow not-so-knight

Furry

Yeehaw but on acid @fire-bay I apologise

owo master

Hrnt and ogm

Minecraft mod sheep and jellyfish

Accident

Worm

And the Gd taMr

[fhccosmetics]

Our natural shampoo bars and soaps are handcrafted to perfection using the “cold process” technique which requires a long period of time and lots patience. https://www.freshhandmadecosmetics.co.uk The oils are infused with fresh herbs for a period of time before making the soap and after the soap is made it needs curing from 4 to 8 weeks. #Shampoobar #naturalsoap #nosls #vegan #shampoo #solidshampoo #plasticfree #Soap #UKGiftAM #SkinCare #eczema #psoriasis #nappyrash #acne #handmade #handmadesoap #herbal #herbalsoap #handcrafted #soaplove #natural #artisansoap #handmadewithlove #giftideas #CrueltyFree #PalmOilFree #zerowaste #naturalcosmetics #zerowasteuk #shampoobars = = = = = = = = = = = = = = = = = = = = = = Nuestros jabones y champú naturales están fabricados a la perfección utilizando la técnica artesanal de "proceso en frío" que requiere un largo período de tiempo y mucha paciencia. Los aceites se infunden con hierbas frescas por un período de tiempo antes de hacer el jabón y una vez que se prepara el jabón, hay que dejarlo curar durante 4 a 8 semanas. https://www.instagram.com/p/B3Raeh1nSUz/?igshid=6a95xs5mwnht

[yestoseraphina-blog]

Yes To Cucumbers... Yes To Life!

We all have seen that movie where the 12-year-old boy and his best friend have a sleepover and spy on his older sister, Mina, when suddenly she turns around and.... her face is GREEN! The boys shriek with terror and run out of the room. 

But why was her face green? 

Well, Mina has sensitive skin and cares about what she puts on her body, so she was indulging in the YesToCucumbers natural based mud mask that is specially designed for those of us with faces that are just a little more sensitive than others. 

Mina also knows that YesTo products are cruelty free, so what she is putting on her face gives her nothing to feel guilty about- except for maybe scaring the life out of her little brother (but I imagine she didn’t hate that part either). 

The YesTo family of skincare products is all about using every day ingredients (that more often than not double as foods!) that are vegan, cruelty free, and natural based and that function without the use of parabens, SLS, or silicones that poison your face. 

YesToCucumbers embodies the YesToHappiness campaign, honoring the desire to do right by your body without compromising quality or functionality. Say yes to happiness in your life and on your face. Be like Mina and start doing single packaged face masks from YesTo every night just in case her little brother tries to come snooping again. Above all, live your best skincare life and personal life, say YesTo you!