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IPKKND SS: There’s More To Her #9

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Awakening

An overconfident Khushi had correctly assumed that Arnav Singh Raizada would be absolutely helpless when it came to finding a location for Akash and Payal’s first date. And Devi Maiya always sided with her favorite devotee, until that devotee gloated in the ego she accused the atheist for.

It was funny that the atheist had no idea how often Devi Maiya sided with him.

Hence, the final, accident free date location was Akash’s office with a variety of snacks that a closed office canteen could offer. Pre packaged brownies, coffee from a coffee maker, tea in a to-go cup and some favourless, tasteless digestive biscuits from Arnav Singh Raizada’s personal stash of snacks along with two bottles of mineral water - of course.

Khushi, though, had worked her magic into Akash’s office. White rose petals scattered on the dark table, all lights were switched off except the fairy light trees and a lone office desk lamp.

Although the effect never reached its recipients as Akash and Payal took their sweet time gazing at each other. Despite her upbringing, Payal let her eyes wander over the well fitted chocolate suit and white shirt on Akash. He was taller and more muscular than she thought. White really was Akash’s color.

Akash was not indifferent to female gaze. While his brother benefitted from the don’t-you-fucking-stare-at-me face, Akash’s politeness and Raizada-ness left him to awkward thank yous and exit strategies from press meetings.

But this was Payal, and all he could feel was dhak dhak.

Khushi, oblivious to her nauseated partner of third wheeling the love birds, played with the fairy lights and begrudgingly accepted that Arnav had otherwise done splendid work.

But before Arnav could recover from his nausea at the puppy eyes Akash and Payal exchanged, he balked at the outfit Akash held for Khushi.

Red saree

“Yeh?” Akash snapped out of his trance at Khushi’s question.

“Oh, yes Khushi ji, isn’t it wonderful that I found this in the wardrobe? Although it is last season,” Akash mused.

Arnav stood rooted to his spot, memories assailing his heart and mind without a drop of mercy.

“Nevermind, I thought you’ll be comfortable in this because you modeled-”

“Modeled?” Payal frowned.

“Haan, that’s why Khushi came home late-” Akash stopped, belatedly realizing Payal had absolutely no knowledge of the disastrous calendar photoshoot that took place on her birthday. Payal shot a look at her younger sister, waiting for an explanation that was overdue.

“Woh, Jiji… I was responsible for looking after Liza-”

“That snooty model?” Payal scrunched her nose in disgust. Khushi nodded,

“Yeah wohi, so I was responsible for her fur coat. I had to hold it still until the shoot was done but somehow it got spoiled so as punishment I had to model in place of her.” Khushi concluded. Payal frowned, Khushi usually told stories that had no head or tails and this one simply took the cake.

Of course there was one man responsible for this.

Arnav Singh Raizada

That man had the grace to look away.

“I appreciate what you have done for me and your brother Arnav-ji, but you’re telling me that a fashion house does not have a single cupboard-” Payal began but Arnav and Khushi sneezed at once, shivering in their wet, unchanged clothes.

“As I was saying-”

“Jiji, there’s no need for this. You and Akash-ji enjoy your date. And if I change my outfit any later then I’ll catch a cold and you don’t want that right!” Khushi grabbed Payal’s arm, begging her to let go of the matter. At the end of the day despite the horrible memories of the day at the office, what mattered more to Khushi was her sister’s office. After months she needed no revival of the challenging days, nor any apologies.

Payal sighed and tapped her hand with reassurance. Khushi smiled and hugged her sister gently, careful her wet clothes didn’t ruin Payal’s new ones, patted Akash’s back and whispered ‘good luck’ before skipping out of the office. Arnav waited until Khushi was out of sight.

“Payall, I know how it seems….” Arnav started. Payal closed her eyes and willed patience.

“I crossed multiple lines-” Arnav said.

“The one to whom  you owe any apology or explanation isn’t in this room Mr. Raizada.” Payal snapped, “and the explanations provided out of questioning instead of conscience have no value in my eyes.” Payal concluded. Arnav’s jaws tightened. For once he wished Payal yelled or debated, it gave him ground to fight.

But one rarely could if they were confronted with righteous anger and dignity. Arnav looked at Akash who chose to remain silent. For many times Arnav counted on Akash’s silence, be it keeping things private or taking reprieve that at least one member of his family wouldn’t harp on him about marriage.

But now, seeing Akash’s silence when he too knew what the events were, left Arnav all alone to face Payal’s anger. Arnav finally realized why Akash rarely involved himself in others’ battles.

It forced accountability.

Payal waited for Arnav to come up with a reason regardless. If he’d launch into long paragraphs, her remaining respect for him would touch the grounds. Because ever since the day her sister came home unconscious in his arms, Payal stopped giving any heed to Arnav Singh Raizada.

It was his love for his family and the lengths he went to get her and Akash together that made him an average man.

So when Arnav said no further and headed for the door, Payal stopped him.

“A final word, if you don’t mind.” Arnav stood still, giving her his attention “I don’t ever want you to respect my sister because there might be a relationship between the two families one day. I don’t want you to think anymore that she’s behind money because it has been proven that every assumption you’ve ever had of her was wrong.”

“When you do respect her, let it be for the fact that a 20  years old middle class woman with a third of your education, wealth and experience has been providing for a family of five which involves three aged people and a sister with a broken marriage.” Payal said, tears betraying the potent love for her sister.

Arnav briefly nodded and left the room.

“You know right, your broken marriage doesn’t define you.” Akash began, giving Payal a glass of water. Payal softly smiled, not having the energy in her tonight to explain it to him that it is how the society defined her.

“I’m sorry, I should’ve started this on a better note.” Payal said after a moment.

“Payal ji, never apologize for doing the right thing. A part of me loves you because of who you are and how fiercely you love your sister.” Akash pulled a seat for her held her smaller hands in his.

“Then what does the other part of you love me for?” Payal added, her reddened cheeks and soft smile mirrored on Akash’s face.

He leaned in as her eyes closed at their proximity, “For how beautiful you are.”

Payal’s eyes flashed open and her nose brushed against his. She removed her hands and played with the hem of her saree, “Really?”

“No, it’s actually the way you scold Bhai.” Payal stared at his blatant response and broke into a laugh as he chuckled at his statement.

– – –

Arnav sat in the cafe in a fresh suit, Payal’s words playing in his mind in a loop. He closed his eyes and sighed. She was right. Every single assumption he had of Khushi Kumari Gupta had been proven false. From the moment she fell into his arms at Sheesh Mahal to when he’d accuse her of visiting Shantivan to charge extra money from his family.

Speaking of Khushi, where was she?

A familiar prick of awareness seeped through him. Goosebumps broke on his flesh. His heart pumped in a staccato rhythm.

“Khushi,” He whispered even before he saw her. With a soft turn he watched her in the darkness of his office. Little beads of sequins reflected the dim lights. The gossamer red saree left little to imagination.

Khushi stopped still, feeling the heat of his eyes on her. She clenched her fists and sat on the nearest chair she could find - farthest away from him. She prayed, prayed to calm her racing heart, feel the chill of the air instead, to stop the dastardly pull towards the one man who would push her off the cliff.

She barely looked behind and sighed in relief and disappointment when he looked away.

For how long must she rot in these emotions?

– – –

“Akash ji, if you don’t mind, I’d like to ask  you a few questions before we…” go any further. Payal hesitated. Akash was more than the man of her dreams and she wondered if she tested his and Devi Maiyya’s patience by putting every single obstacle instead of accepting the gift of love.

From denial to innumerable questions, was she jinxing her relationship even before it began?

“Please do Payal ji.” Akash smiled, worried at her hesitation. As much as he believed love was at first sight and that he would never regret having Payal in his life, he wondered if he had pushed her into accepting her love. He should’ve waited. True love didn’t come from desperation, it came from patience.

There was still a great distance between them. It was strange. He had thought an ��I love you’ from both the ends sealed the deal. But now that they lapsed into awkward silence around difficult questions, he wondered if he had ruptured things by hastening it out of fear.

“Akash ji, I do… love you,” Payal confessed, worried at his sudden silence, “but I won’t find any comfort in this love until certain things are clear. Please understand.”

“I absolutely do Payal ji. Please feel free to ask about anything.” Akash reassured her.

“What’s yours and your family’s take on dowry?” For a second Akash was offended at her question. Didn’t she know how much he loved her? How he and his family would never take a cent for such a crime! But looking at her frightened eyes he was ashamed at feeling offended. He had the luxury of never being associated with such folks. She had lived through this.

“The Raizadas have never given nor taken a cent. We did make the mistake of offering a property and farm lands as gifts for one wedding, it is perhaps no surprise things didn’t end up well.” Akash’s mouth soured at the memory of the Maliks. He had never liked that side of the family, even as a child. When he had helped Arnav get back his mother’s property from the Maliks - one Arnav’s uncle had in possession - Akash was glad that his cousin never grew up in that environment.

His Dadi’s friendship and care for Arnav’s Dadi was the only sorepoint between Arnav and Akash. Akash never approved of his brother spending hard earned money behind a member of the family that had only hurt the Raizadas and above all, hurt his dearest cousins. Although his inner emotions couldn’t be revealed to Arnav at a worse time.

“I cannot believe this Papa. Dadi-ji sent Anjali-di to dissuade Bhai from buying Sheesh Mahal!” Akash fumed, clenching his fists to reign in his temper.

“Subhadra Devi-Ji had called Amma. It is the last house her son has.” Mahinder sighed, weary of the fight he had with his own mother, “And Amma said it’s not right for Arnav bitwa to treat ancestral property as a tool for revenge-”

“That property is ours. Phupha-ji got it as dowry. If anything, Bhai and Di deserve to have it back. We’re paying for that woman’s extravagant ashram, it’s enough.” Akash said.

Mahinder shook his head and patted his young son’s shoulder. It was rare to see him so worked up, even for the right reasons, “Akash bitwa, aisa nahi bolte. You know that Amma believes her friend has a good heart after all-”

“I won’t let them misuse Bhai’s newfound fame. I don’t know why Dadi forgets she’s a Malik. That family sucked us dry. I hate it that we have an association with them-” Akash stopped, noticing Arnav on the door, his face ashen.

Arnav said nothing, nearly pretended he heard nothing. He simply lavished Mami with an unlimited access of jewelry from his bank account, Mama was given a significant share from his company, and stopped any of the Raizadas from contributing to Subhadra Malik’s expenses.

Akash knew. That even in a slip of tongue, Arnav took the words to heart and paid the Raizadas ten fold for any kindness they ever showed.

And because Arnav wouldn't accept any apology - for his side of the family was terrible - Akash vowed to stand next to him and never make him feel ‘othered’.

Payal held Akash’s hand, her heart melting at Akash’s sincerity and stories of the extent Arnav went for the family.

“Bhai has quietly become the head of the family. It’s not arrogance, it’s a fact. He looks after everything. And when he has his blessings over this relationship, no one and nothing can come in between because he simply won’t allow it.” Akash reassured her. Payal smiled, finding some hope in the marriage ahead of her.

Perhaps Arnav’s rejection of tradition and Akash’s genuine love was all she needed. To think of it no one apart from his mother scrunch their noses about their class difference. True, at times they were a bit blind in privilege, but that also stemmed from harmlessness.

– – –

Arnav kept looking behind, losing his heart every time he saw the flash of red. He had rarely seen Khushi so quiet. After ten uncomfortably silent minutes, he turned to find her engrossed in some books, highlighting something with a pencil.

It was a mathematics textbook. For children.

Khushi looked up, aware of his questioning gaze and cleared her throat, “Woh… I tutor kids so I’m preparing materials in advance.” Arnav took that as an offer to walk towards her. Khushi tucked back a strand, nervous at their little talk.

“Mathematics?” He asked.

“Yes, I was studying financial management so mathematics had to be my favorite subject. According to Babuji finance is the backbone of any successful venture.” Khushi smiled, recalling her days in college and at the sweet shop. Arnav realized he truly didn’t think about Khushi’s life before they met.

“But I haven’t seen you attend college…” Arnav said, sitting next to her.

“I dropped out.” Khushi said and plastered a big smile on her face, “Anyways according to Babuji real life experiences shape you more than education. So between running a sweet shop in the morning, tutoring kids in the evening and managing orders for Buaji’s chikankari sarees, I’m running quite the enterprise here.” Khushi went back to her book, happy at the way her life shaped. Of course, she’d never want Babuji's ill health, but if she was in Lucknow then she would’ve happily left her college to marry right after Payal did.

It never crossed her mind that there was more to life. There was more to her.

Arnav couldn’t manage to return her smile. It didn’t take a genius to figure out why she couldn’t complete her college. Between the scandal, the loans, resettling to a new city, financial pressure and her father’s health - Khushi’s education would’ve taken the back seat.

And he was responsible for nearly all of it.

“You know, AR is hiring-”

“No.” Khushi said, “I cannot work here.”

“No one refuses Arnav Singh Raizada.” Arnav glared.

“Accha. No, no, no, no.” Khushi stuck her tongue. If only Khushi knew that she was far from annoying, rather quite adorable.

“So what happened to your Babuji’s business… now that you’re running on your own?” Khushi couldn’t believe if Arnav was actually engaging in small talk. It felt new. It felt good. It had been too long since anyone asked her about her life.

“Well they were seized by the loan sharks-” Arnav frowned, “-woh Babuji had given it up for loan for Jiji’s dowry to loan sharks. And then the people refused to return the store back considering Babuji’s fallen goodwill. The scandal-” Khushi grew quiet and Arnav cursed under his breath.

The more he spoke to either of the sisters, the more he learned about the things he wished he didn't.

– – – – – – – –  

“I genuinely loved the dupatta!” Payal gushed over the intricate fabric designing and detail with Akash, enjoying the moment as they bonded over their shared love of textiles. Akash was the finance man, but he was enamored by the beauty of embroidery and the texture of fabrics - except he never had the talent to actually make anything out of it. Although to Arnav that was an asset, Akash would always know if their materials were genuine or if they were being scammed.

“If I knew it was you I would’ve packaged it better!” He confessed. Payal was touched. The fact that Akash was ready to give it a stranger because it would brighten Khushi’s day meant everything. She had seen fate play on someone’s goodness, it was surprising to see someone’s goodness move fate.

“Would you mind if I worked after marriage?” Payal asked.

“Do you want to?” He asked.

“I don’t know. I’ve never seen myself there. I’ve never had ambitious dreams,” Payal admitted. Akash empathized. Ambition was never his asset. On the other hand Khushi and Arnav had heaps of it.

“Payal ji, I might’ve been born with a silver spoon but all I’ve ever wanted is good work and a happy life. Really. And maybe a dog one day.” Akash mused.

“A dog? In a house where there’s a lamb?” Payal chuckled.

“Why, don’t you want one?” Akash asked.

“I’d get another lamb, or maybe a little dove. Khushi loves doves and seeing doves would remind me of her. But… I wouldn’t want them in a cage.” Payal said.

“Bhai’s garden is big enough for a sanctuary for a few birds, but I’m wondering what he would say when they would leave droppings in his pool!” Akash laughed.

“Aur kya kahenge? What the?” Payal growled, imitating Arnav to perfection. Akash clapped at the mimicry and shook his head, “It looks like I’ll have to create a garden for the doves.”

“And I’ll have to teach the dog to be calm - especially around the doves, Lakshmi and future children.” Payal dreamed.

“Children?” Akash asked.

“Kyun, you don’t want any?” She questioned. Akash did look like he’d be an excellent father. Graceful, patient, calm and a listener. If that wasn’t like Babuji, who else was?

“Only if they look like you.” He smiled as Payal turned a pretty red in shyness. Akash brushed a tendril away from her face and stroked her cheek with the back of her fingers.

“Akash-” the lights went off, leaving them in the darkness.

– – – – – – – –

“Hey Devi Maiyya!” Khushi gasped as she couldn’t see anything. Before she could move, Arnav's hand found hers.

“It’s ok Khushi. The generator should kick in soon.” Arnav’s firm voice instantly calmed her nerves. It was alright. He was here. She is not alone. Her nails dug into his skin. Her palms had turned clammy.

To Khushi’s relief Arnav switched on his phone’s flashlight.

“Fuck,” Arnav swore under his breath. His phone battery was at ten percent. Why wasn’t the generator working?

“Khushi, wait here. I’ll go the studio and grab some candles-”

“Nahi, please…” Khushi gripped his hand tighter.

Arnav tugged her along. They made it to the fusebox, a candle in place of the dying flashlight. For a moment Khushi was arrested by his face lighting up by the flame. His expressionless face was scrunched in worry. Arnav realized he was being watched.

“Kya hua? Tum theek ho?” Khushi nodded and looked at the board. Strange, everything looked fine. Even the fuse was intact.

Arnav leaned by her, Khushi stiffened at the proximity. He bent to her eye level, looking at all the switches. She could feel his breathing, the heat his body emanated.

“Oh of course! I completely forgot that we had the electricity maintenance-” Arnav stopped as his nose brushed against hers, their faces close up next to each other. Words died out as her large eyes looked deep into his. His eyes flickered to her lips and her breath hitched.

He gulped as her lips parted. It had been so long since something right had happened. Memories of Diwali and the photoshoot intermingled with his hidden fantasies. Time lost its essence to Khushi. All she could see was him, his eyes and his lips. Desire flowed like blood through her veins. There was so much tension in his face. If she could only brush it away.

Arnav leaned in, without rationale, and Khushi inched up, on instinct. His arm slipped to her waist and her hand rose to his cheek. Arnav closed his eyes at the touch of her fingertips but froze, feeling the metal band of her ring on his cheek.

Khushi’s trance snapped, the diamond of her engagement ring shining brightly in the candlelight. Horror flooded her face. What was she about to do? Arnav sprang back, his breath harsh. What had he just done? The lights came back to life, revealing their darkest desires to each other. Without a word Arnav turned around and rushed out of the room.

– – – – – – – – – –

Akash and Payal exchanged the sweetest glances across the rearview mirror as Arnav drove them all home. A slow romantic ballad played on the radio and Arnav held the steering wheel in a tight grip. Khushi stared out of the window, bothered by her own immorality.

He was Lavanya’s! She was betraying her best friend.

Khushi saw the stars above, twinkling in the clear night and she didn’t have the courage to meet them. She had sinned. For he was anothers.

Who would help her in this crisis? Who could?

Payal reached out and squeezed Khushi’s hands. She had seen Khushi look lost throughout the day. She gestured if everything was alright with her - Khushi only smiled back in response. She didn’t even realize when they stopped near Preeto’s house at an alley where they could be safely dropped off without any nosy neighbors.

Akash led Payal down the SUV, his heart hammering with joy and anticipation. So much was said and so much was left to be said. Payal’s mind urged her to hug before they left, but her sensibilities of society prevented her from doing so.

Khushi realized after a moment that she and Arnav were alone in the car. She immediately tried to remove her seatbelt but fumbled in her nervousness. Arnav walked around, opened the door and removed her seatbelt. His eyes no longer bore warmth. Khushi knew very well what that look meant. She ignored his hand and stepped out of his car.

“Just so you-”

“For the love of God don’t say anything.” Khushi warned him, anger sitting as tears in her eyes. She could not bear another word. Arnav stood still, clenching his jaw. He didn’t know if he intended to give an explanation to her or to himself.

“Akash!” Payal called out to Akash, and the shy lovers met for a hidden hug behind the car. At first there was hesitation, which slowly melted as he leaned awkwardly to match her height.

Khushi pulled the edge of her saree, it was minutes away from ripping. Arnav rolled his eyes and moved to tug it free but she stepped away,

“Stay away,” She hushed, aware of Akash and Payal.

“It would’ve ripped-”

“Then let it rip, aapko kya farak padta hai?” She snapped, pulling off her saree that left a little tear. Arnav growled, he wasn’t an idiot to not realize that this conversation was a painful continuation of the ones left abruptly before.

Yet, his ego stung.

“Don’t get any ideas, you mean nothing-”

“Exactly! You’re Lavanya ji’s, we must never meet again.” Khushi decided, tears of anger flowing down her cheeks. Burned, Arnav got into the car and slammed it shut - shocking Akash and Payal who embarrassedly pulled away, cheeks tinged in red.

Payal mistook Khushi’s urgency as the threat of Buaji questioning the delay and Akash misunderstood Arnav’s urgency as his usual nausea to affection.

Long after Arnav reached Shantivan, he paced throughout his room, a niggling doubt solidifying over time. Ever since he and Khushi were engaged - to different people - Khushi always mentioned his engagement. For someone very chatty, she spoke about anyone and anything except her fiance.  

You’re Lavanya ji’s…

So what about Khushi? Who did she belong to in this world of commitment? Arnav was aware of the arranged setup that seemed to have been exchanged between Khushi and her fiance… but if he could read her well, she just didn’t want to not marry, she despised it.

She despised her fiance.

“Khushi… what the hell are you doing?” He murmured, unaware of the irony.

But it wasn’t lost on Akash who stood by the door, listening to Arnav’s murmur. The discord he long suspected between Arnav and Lavanya was not due to the former’s commitment issues or the latter’s drastic change of opinion of marriage. 

Arnav had tragically fallen in love with the wrong person at the wrong time.

– – – – – – – – – – – –

Payal paced in front of the bathroom, her concern growing as Khushi took a late evening shower in cold water.

“Haye re Nandkisore! We barely have water saved and the queen needs to take a bath after a simple outing!” Madhumati complained. She was touched by the exclusive gifts Preeto had given Payal and Khushi - the yellow and red saree looked exquisite! And laughed at the kids' childishness to wear the gifted outfits and come home.

“Buaji, let Khushi ji be.” Shyam sweet talked to Madhumati, both sharing a secret smile over what happened minutes ago. And for all his honeyed words - he couldn’t match Payal’s suspicious eyes.

“Khushi, you look beautiful.” Khushi was startled as Shyam dropped the ji, walking incredibly close to her. Panicked, she looked around to find Buaji smiling and turning around - giving the couple their ‘privacy’. After all, to-be-newlyweds must spend some time together!

Shyam looked around and then grabbed her wrist,

“No one is watching us, don’t worry.” His fingers traced her arm.

“S-Shyam ji, this isn’t right!” Khushi snatched her hand back.

“Why? We’re engaged aren’t we?” He smiled. Khushi balked at the predatory glint in his eyes and started to sweat. A hand moved to her face-

Khushi splashed the water over herself, shaking as the cold seeped into her body. What if Payal hadn’t interrupted him? She scrubbed the soap over her wrist multiple times. The touch… it felt wrong. She muffled her sob, cleaned up and got out of the washroom - giving Payal her best smile.

Payal couldn’t return it. Shyam and Khushi were a couple. Yet what she saw, even in the flicker of a second, frightened her.

It looked anything but romantic.

Madhumati and Garima were deep into a conversation, they saw Khushi and stood up. Fear crawled in Khushi’s stomach. Every single time the two had something to say to Khushi it was the worst.

“Khushi, Lata saw you and Shyam babua together at the patio. Granted you two are marrying but everyone must keep boundaries. Shyam babua is very kind and mature enough to promise not to visit anymore in the evening.” Khushi wanted to protest, minutes ago Madhumati had been the one who left them alone!

“And hence, a decision has been made.” Madhumati spoke, giving little room to anyone else present.

“In three weeks you and Shyam babua will be married.”

- - - 

A/N: Hey there! Thank just in case if you actually remember the story! I’m finally back with an update and I sincerely hope you liked it - just a couple of chapters left for me to wrap this up!

- JWB

Tagging: @butaneandthebeast @shaonsim @shiyaravi @myloveforstuff @leila1 @lostafpanda@laadgovernorandsankadevi @laad-governess @thenainitaldisaster @maansiloves@chutkiandchotte @honeybellexox @bigfatreader @simplycurlz @thecharlesboyle@sapnokiduniyaisalwaysbetter @zaphbeeblebrox @shehzaadimira @pakki-ya-nahin@mindpdnim @jaanedil @pandora-678 @featheredclover @rae-blogging @goals1024@onadaanparindey @magicfeltmybloop @acroakingbird @blogenana @starzin8s@exosexosekai @noor1025 @ijustchangedmyname@delicatecloudfestivalweasel @sankititaliya @dvk-ok @roqiaposts​ @white-thebeauty​ @childofsquidward​ @maansiloves​ @shehzaadimira​ 

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The National Emergency Management Agency (NEMA) on Saturday in Abuja https://ift.tt/2ACAHQ6

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The $56 Million Mansion at 9 East 71st Street Where Epstein Allegedly Abused Girls https://www.nytimes.com/2019/07/08/nyregion/jeffrey-epstein-nyc-mansion.html

Inside Epstein’s $56 Million Mansion: Photos of Bill Clinton, Woody Allen and Saudi Crown Prince

The townhouse where the financier Jeffrey Epstein is accused of abusing girls is one of the largest private homes in Manhattan.

By Matthew Haag | Published July 8, 2019 | New York Times | Posted July 8, 2019 |

The townhouse where the financier Jeffrey Epstein is accused of engaging in sex acts with underage girls is one of the largest private homes in Manhattan, a short walk from Central Park.

The seven-story residence at 9 East 71st Street, between Fifth and Madison Avenues, sprawls across 21,000-square feet and has five bathrooms, a two-story reception room and many bedrooms, including three three-room suites on the fourth floor.

It also has a heated sidewalk in front to melt the snow during the winter months. On the second floor, Mr. Epstein hung a mural he had commissioned in recent years: A photorealistic prison scene that included barbed wire, corrections officers and a guard station, with Mr. Epstein portrayed in the middle.

“He said, ‘That’s me, and I had this painted because there is always the possibility that could be me again,’” said R. Couri Hay, a public relations specialist invited by Mr. Epstein to a meeting at his home and to view the mural three months ago.

A person who visited the townhouse last year remembered Mr. Epstein doing work at the mansion while seated at a large dining table in the rear of the house that fit 20 people, with multiple computer monitors and a phone alongside.

Mr. Epstein answered calls while hosting visitors, according to that person and another visitor last year who saw similar behavior. Behind him was a table covered with framed photographs of celebrities and dignitaries, including a signed photograph of former President Bill Clinton.

The visitors each said they were greeted by a tall woman who spoke with an Eastern European accent, who led guests up a marble staircase to a study on another floor. At the base of the stairwell, one of the visitors said, Mr. Epstein had placed a chess board with custom figurines dressed in underwear — each piece, he noted, was modeled after one of his staffers.

He decorated the home with other oddities, like a life-size female doll in a wedding dress hanging from a chandelier, and had arranged a small dining room to resemble a beach scene.

A wall of the study was covered with photographs of famous people, including Woody Allen, and another that Mr. Epstein pointed out was of Crown Prince Mohammed bin Salman of Saudi Arabia.

Mr. Epstein, 66, is accused of engaging in sex acts with young girls from 2002 to 2005 during naked massage sessions and paying them hundreds of dollars in cash, according to an indictment unsealed on Monday.

During the search of his townhouse on Saturday, investigators seized nude photographs of underage girls, federal prosecutors said. “The alleged behavior shocks the conscience,” Geoffrey S. Berman, the United States attorney in Manhattan, said on Monday.

Prosecutors said they were moving to seize Mr. Epstein’s townhouse.

Mr. Epstein, however, was not even supposed to become the owner of the opulent stone house on the Upper East Side.

In 1989, Mr. Epstein’s mentor, Leslie H. Wexner, the founder and chairman of L Brands, the parent company of Victoria’s Secret and Bath & Body Works, bought the seven-story Beaux-Arts home for $13.2 million. At the time, it was the highest recorded sale price for a townhouse in Manhattan.

Mr. Wexner then spent at least that much on artwork — including multiple works by Picasso — Art Deco furnishings, Russian antiques, rosewood tables and doors and a gut renovation of the home. Security devices, including a network of cameras, were installed. A cellar was divided into separate spaces, one for red wines and another for white. The renovation was featured on the cover of the December 1995 issue of Architectural Digest.

The townhouse had been a longtime private school and Mr. Wexner spent years converting it into a lavish estate.

Mr. Wexner, however, never moved in; he decided to stay in Columbus, Ohio, where L Brands has its headquarters.

But another person did move in: Mr. Epstein. “Les never spent more than two months there,” Mr. Epstein told The New York Times in 1996.

The home has a history of going unoccupied by its owner. Herbert N. Straus, an heir to the Macy’s fortune, commissioned the 40-room mansion in the early 1930s and hired the prominent architect Horace Trumbauer to design it. But Mr. Straus died in 1933, leaving the property unfinished and unoccupied.

The Straus family gave the residence to a hospital in 1944. In 1962, the private school, Birch Wathen School, bought it and converted it into a schoolhouse. The school, which was started in 1921 with an emphasis on the arts, relocated after Mr. Wexner bought the house. (It later merged with another private school to start the Birch Wathen Lenox School.)

Mr. Epstein said in the 1996 interview that the mansion was now his, though the transaction has never appeared in New York City records online. In 2011, he transferred ownership of the property from a trust connected to Mr. Epstein and Mr. Wexner to Maple Inc., a United States Virgin Islands-based entity under Mr. Epstein’s control, according to records.

The transfer document, from Nine East 71st Street Corporation to Maple Inc, did not list a purchase price, indicating that it did not involve any exchange of money.

A spokeswoman for Mr. Wexner said that he “severed ties” with Mr. Epstein about a decade ago. Erika Kellerhals, a lawyer in the Virgin Islands who handled the 2011 transfer for Mr. Epstein, did not return a phone call seeking comment.

Prosecutors say the home is valued at $77 million, but the city’s Department of Finance estimated earlier this year that the mansion was valued closer to $56 million. The 2019 property taxes for the home, which are based on a much lower assessed value, were an estimated $347,000. (The property’s tax bill in 2008 was the fourth highest-taxed single-family home in New York City.)

Mr. Epstein also owns a home in Palm Beach, Fla., a 7,500-acre ranch in New Mexico and an apartment in Paris. He also owns Little St. James Island, a private island in the Caribbean, and at least 15 cars.

A spokesman for Mr. Clinton, whose photograph was on display in Mr. Epstein’s mansion, said that the former president “made one brief visit” around 2002 to the New York home with a staff member and security officials. Mr. Clinton has not talked to Mr. Epstein in more than a decade and has not visited his properties in the Caribbean, Florida or New Mexico, the statement said.

From the street, Mr. Epstein’s Manhattan mansion has features that make it a commanding presence on the block: a 15-foot-tall oak front door (which the police pried open with a crow bar on Saturday during a search of the property), large arched windows on the ground floor and a balcony on the second floor. Mr. Epstein’s initials are on a brass plaque near the front door.

While Mr. Epstein has tried to maintain a private life, he did allow a reporter for Vanity Fair magazine to visit the mansion for a 2003 profile, “The Talented Mr. Epstein.”

The article describes a main hallway that was covered with rows of artificial eyeballs from England that had been made for wounded soldiers.

The room connected to the hallway was a marble foyer adorned with a painting that resembled works by the French artist Jean Dubuffet. “The host coyly refuses to tell visitors who painted it,” the reporter, Vicky Ward, wrote.

The article was published around the same time that he started to recruit underage girls to the mansion, according to the indictment unsealed on Monday.

Nellie Bowles, Matthew Goldstein and Sarah Maslin Nir contributed reporting.

[layleesres-blog]

Keto Fit go

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[greatreviewreview]

What to know about peptides for health

Peptides are smaller versions of proteins. Many health and cosmetic products contain different peptides for many uses, such as their potential anti-aging, anti-inflammatory, or muscle building properties.

Recent research indicates that some types of peptides could have a beneficial role in slowing down the aging process, reducing inflammation, and destroying microbes.

People may confuse peptides with proteins. Both proteins and peptides are made up of amino acids, but steroids powder contain far fewer amino acids than proteins. Like proteins, peptides are naturally present in foods.

Due to the potential health benefits of peptides, many supplements are available that contain peptides that manufacturers have derived either from food or made synthetically.

Some of the most popular peptides include collagen peptides for anti-aging and skin health, and creatine peptide supplements for building muscle and enhancing athletic performance.

In this article, we discuss the potential benefits and side effects of peptide supplements.

Peptides are short strings of amino acids, typically comprising 2–50 amino acids. Amino acids are also the building blocks of proteins, but proteins contain more.

Peptides may be easier for the body to absorb than proteins because they are smaller and more broken down than proteins. They can more easily penetrate the skin and intestines, which helps them to enter the bloodstream more quickly.

Scientists are most interested in mechano growth factor peptide, or those that have a beneficial effect on the body and may positively impact human health.

Different bioactive peptides have different properties. The effects they have on the body depend on the sequence of amino acidsTrusted Source they contain.

Some of the most common peptide supplements available are:

Collagen peptides, which may benefit skin health and reverse the effects of aging.

Creatine peptides, which may build strength and muscle mass.

Some people may take other peptides and peptide hormones to enhance athletic activity. However, the World Anti-Doping Agency have banned many of these, including follistatin, a peptide that increases muscle growth.

Collagen is a protein in the skin, hair, and nails. Collagen peptides are broken down collagen proteins that the body can absorb more easily. Taking collagen peptides may improve skin health and slow the aging process.

Some studiesTrusted Source indicate that dietary food supplements that contain collagen peptides can treat skin wrinkles. Other research indicates that these supplements may also improve skin elasticity and hydration.

Peptides may stimulate the production of melanin, a skin pigment, which may improve the skin’s protection against sun damage.

Topical anti-aging cosmetics can also contain Melanotan Peptide, which manufacturers claim can reduce wrinkles, help skin firming, and increase blood flow.

Improve wound healing

As collagen is a vital component of healthy skin, collagen peptides may facilitate faster wound healing.

Bioactive peptides can also reduce inflammation and act as antioxidants, which can improve the body’s ability to heal.

Research is currently ongoing into antimicrobial peptides, which may also improve wound healing. Having very high or very low levels of some antimicrobial peptides may contribute to skin disorders, such as psoriasis, rosacea, and eczema.

Prevent age-related bone loss

Animal research links a moderate intake of collagen peptides with an increase in bone mass in growing rats who also did running exercise.

The study may point to collagen peptides being a useful way to counteract age-related bone loss. However, more research is necessary, especially on humans.

Build strength and muscle mass

Some researchTrusted Source on older adults indicates that collagen peptide supplements can increase muscle mass and strength. In the study, participants combined supplement use with resistance training.

Creatine peptides may also improve strength and help to build muscle.

While fitness enthusiasts have been using creatine protein powders for many years, creatine PEG MGF peptide are increasing in popularity.

These particular peptides may be easier for the body to digest, which means they may cause fewer digestive problems than creatine proteins.

For healthy individuals, peptide supplements are unlikely to cause serious side effects because they are similar to the peptides present in everyday foods.

Oral peptide supplements may not enter the bloodstream as the body may break them down into individual amino acids.

In one studyTrusted Source where females took oral collagen peptide supplements for 8 weeks, the researchers did not note any adverse reactions.

However, the United States Food and Drug Administration (FDA) do not regulate supplements in the same way they do medications. As a result, people should exercise caution when taking any supplements.

Topical creams and ointments containing peptides may cause skin symptoms, such as skin sensitivity, rash, and itching.

Individuals should always buy from a reputable company and discontinue use if adverse reactions occur.

Also, it is a good idea to speak to a doctor before taking peptide supplements or using topical products that contain peptides.

Those who are pregnant, breastfeeding, taking medications, or living with a medical condition should avoid using peptides until they speak to their doctor.

The timing and dose of peptide supplements will vary, depending on the type and brand.

Always follow the package instructions when taking peptide supplements or using topical peptide creams or lotions. Never exceed the recommended serving size. Discontinue use and consult a doctor if adverse reactions occur.

Peptides are naturally present in protein-rich foods. It is not necessary to take peptide supplements or use topical sources of peptides.

However, some people may wish to use collagen peptides with the aim of slowing down the aging process. Others may take creatine peptides to build muscle and strength.

There is still limited evidence to indicate that these products are effective, and much more research is necessary to assess their efficacy and safety thoroughly.

Research into peptides is in the early stages, and in the future, scientists may discover health benefits of different types of peptides. Until then, people should exercise caution when taking any supplement and discuss the potential benefits and risks with their doctor beforehand.

Protein–protein interactions (PPIs) execute many fundamental cellular functions and have served as prime drug targets over the last two decades. Interfering intracellular PPIs with small molecules has been extremely difficult for larger or flat binding sites, as antibodies cannot cross the cell membrane to reach such target sites. In recent years, peptides smaller size and balance of conformational rigidity and flexibility have made them promising candidates for targeting challenging binding interfaces with satisfactory binding affinity and specificity. Deciphering and characterizing peptide–protein recognition mechanisms is thus central for the invention of peptide-based strategies to interfere with endogenous protein interactions, or improvement of the binding affinity and specificity of existing approaches. Importantly, a variety of computation-aided rational designs for peptide therapeutics have been developed, which aim to deliver comprehensive docking for peptide–protein interaction interfaces. Over 60 peptides have been approved and administrated globally in clinics. Despite this, advances in various docking models are only on the merge of making their contribution to peptide drug development. In this review, we provide (i) a holistic overview of peptide drug development and the fundamental technologies utilized to date, and (ii) an updated review on key developments of computational modeling of peptide–protein interactions (PepPIs) with an aim to assist experimental biologists exploit suitable docking methods to advance peptide interfering strategies against PPIs.

Delivering drugs specifically to patient neoplasms is a major and ongoing clinical challenge. Function-blocking monoclonal antibodies were first proposed as cancer therapies nearly four decades ago. The large size of these molecules hindered their commercial development so that the first antibody or antibody-fragment therapies were only commercialized for cancer therapeutics and diagnostics 20 years later [1,2]. A classic development during this period, a radiolabelled peptide analog of somatostatin (SST) was used to target neuroendocrine tumors expressing the SST receptor instead of targeting the receptor with an antibody [3]. The concept of using a peptide as a targeting moiety for cancer diagnosis and treatment has since led to current peptide drug developments in both academia and pharmaceutical industries. In addition to cancer treatments, melanotan 2 peptide that mimic natural peptide hormones also offer therapeutic opportunities. Synthetic human insulin, for instance, has been long exemplified for its clinical efficacy for diabetic patients [4].

In comparison to small molecules, such as proteins and antibodies, peptides indeed represent a unique class of pharmaceutical compounds attributed to their distinct biochemical and therapeutic characteristics. In addition to peptide-based natural hormone analogs, peptides have been developed as drug candidates to disrupt protein–protein interactions (PPIs) and target or inhibit intracellular molecules such as receptor tyrosine kinases [5,6]. These strategies have turned peptide therapeutics into a leading industry with nearly 20 new peptide-based clinical trials annually. In fact, there are currently more than 400 peptide drugs that are under global clinical developments with over 60 already approved for clinical use in the United States, Europe and Japan.

Protein–protein interactions (PPIs) are the foundation of essentially all cellular process. Those biochemical processes are often comprised of activated receptors that indirectly or directly regulate a series of enzymatic activities from ion transportation, transcription of nucleic acids and various post-translational modifications of translated proteins [7]. Drugs that bind specifically to such receptors can act as agonists or antagonists, with downstream consequences on cellular behavior. Peptides and small molecules that interfere with PPIs are thus in high demand as therapeutic agents in pharmaceutical industries due to their potential to modulate disease-associated protein interactions. Accumulating evidence has suggested that better identification of targetable disease-associated PPIs and optimization of peptide drug binding characteristics will be key factors for their clinical success [8].

Unfortunately, understanding the molecular recognition mechanism and delineating binding affinity for PPIs is a complex challenge for both computational biologists and protein biochemists. This is largely because small molecules are superior in binding to deep folding pockets of proteins instead of the larger, flat and hydrophobic binding interfaces that are commonly present at PPI complex interfaces [9]. Although monoclonal antibodies are more effective at recognizing those PPI interfaces, they cannot penetrate the cell membrane to reach and recognize intracellular targets. In recent years, peptides with balanced conformational flexibility and binding affinity that are up to five times larger than small molecule drugs have attracted enormous attention [10,11]. Cyclic peptides, for example have small molecule drug properties like long in vivo stability, while maintaining robust antibody-like binding affinity and minimal toxicity [12]. In this review, we will focus two aspects of peptide drug development: (i) Fundamental technologies utilized for peptide drug developments to date, and (ii) key developments of computational modeling techniques in peptide–protein interactions (PepPIs). Recent topics and basics in conventional docking of PPIs will also be covered with an aim to assist experimental biologists exploiting suitable docking methods to advance peptide interfering strategies against PPIs.

[sissystevenblog-blog]

My Experience With Luna Trim

However the influences of this object are of excessive quality while contrasted and other weight dropping items. Luna Trim offers you an augmented fat blazing and a fat less incline body in extraordinarily restricted time. It is produced using concentrates of an exceedingly compelling weight dropping natural product referred to as Garcinia Cambogia.

The substance of Luna Trim scribble down to dispose of the extra present in your frame from the principle day itself. HCA, the pleasant solving can affect the weight loss to your frame by controlling the stages of a neurotransmitter known as serotonin. Serotonin is in charge of feeling hungry, rest examples and nation of mind manage. HCA controls the levels of Serotonin which as a consequence decreases your craving and you eat the negligible nourishment that is required for survival and fundamental vitality wishes. Alternate substance clean your gut and digestive framework and evacuate the fat that receives saved there and changes over them into starches that are utilized by the body for power technology. Really, Luna Trim decreases the limit of move machine to ingest any new glucose moreover it sell continues the procedure of annihilation of starch within the stomach.

 My Experience With Luna Trim

 It become a supernatural occurrence that I saw on myself after making use of Luna Trim . All of a surprising subsequent to turning 30 Years my weight started to shoot up. Before long I resembled a matured female conveying bunches of undesirable fats swinging from distinctive components of my frame. Luna Trim helped me to decrease my middle via 4 crawls in 2 months. This changed into a resultant due to a diminished of 20 Pounds of my weight. Presently, I can with out much of a stretch fit into my clothes of the extra mounted times when I looked skinny. Once extra I recaptured my childhood days of getting a skinny body with Luna Trim .

 Measurements

 The plentiful measure of prescribed HCA each day for us is 500mg – 1000mg. This object has 60 instances every of 500mg. You ought to eat 2 cases for every day preserving an meantime of eight hrs between the 2 measurements.

 Dangers

 Luna Trim augments weight reduction and minimizes and completely takes out any risks which might be linked with weight loss. This item is of excellent models and produced in a GMP showed workplace. It is a characteristic blend and reasons no damage to your body.

 Things to don't forget

 Don't over devour this object

 Pregnant girls ought not take this association

 Devour percent substance inside 30 days

 Store this item in a groovy vicinity

 Entirely take after the recommended dose

 Not ideal for kids

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[sayitwithsarcophilus]

Have some aliens

Same universe as Olivia, Belsameth, and all that crowd.  Here are a few of the intelligent species of the Orion Arm.  Content warning for bad declensions.

Hamadryads/Vanaree (Nymphe sylvestris): “Fuzzy green wood elves.”  Tree-dwelling mammalian humanoids, very physically and psychologically similar to humans but adapted for the forests instead of the savannah.  Have a very plant-based material culture and a strong permaculture tradition.  Their homeworld is popular with human tourists, because even when exploring the wonders of the universe, most people want local food they can digest and furniture that fits their bodies.  The species most commonly involved in human/alien romances.  Hamadryads are usually pretty friendly towards off-worlders and get along well with most other civilized species, but they have some tensions with the Lianenshi.  Hamadryads are not the only sapient species on their homeworld, but the others (the aquatic humanoid Nereids and the elusive marmoset-like Kodama) don’t leave the planet often.

Lianenshi (Sidhe splendens): If Hamadryads are primate wood elves, Lianenshi are paravian high elves.  Lianenshi are tall, thin, bird-like humanoids who resemble a cross between anime characters and Polish Bantam chickens.  Adult male Lianenshi have more colorful plumage and more impressive crests than the females, but otherwise the sexes look very much alike.  They are one of the oldest spacefaring civilizations, very technologically and culturally advanced, and most of them will never let you forget it.  However, they are birds, and even the most subtle and refined Lianenshi aesthetics tend toward the sparkly.  Most Lianenshi have some degree of low-level psionic ability, but psychics with enough power to be useful or dangerous are fairly rare.

R’lyehans (Tentaculum polychromatum): Sapient cephalopods with mad scientist tendencies and mischievous senses of humor.  R’lyehans are skilled at medicine and biology and use a lot of organic technology, since metalworking is impractical underwater.  They are generally curious about other species and interested in interacting with them, but communication can be a problem because the R’lyehan language requires ten flexible arms and the ability to change the color of one’s skin at will, while R’lyehans lack vocal cords and have rather poor hearing, especially outside of water.  (Fortunately, R’lyehans can learn to understand human forms of sign language and vice versa, and translation technology exists.)

Mi-go/MkkEE-g’ourr (Chirurgus frigidus): The Fun Guys From Yuggoth.  Cold-loving winged arthropods, similar to Sheltie-sized Japanese hornets with a touch of crawdad, and covered with silvery-iridescent symbiotic fungus resembling hoarfrost.  Culturally speaking, they borrow more from Discworld’s Igors than their brain-stealing Lovecraftian namesakes, as a race of Creepy Good master surgeons.  (Not that they’d turn down a perfectly good brain if someone was offering, but they don’t go around stealing them.  That would be hideously rude!)  Mi-go are best known for their medical expertise, but their gently eerie music (not too dissimilar from human drone and dark ambient) is also popular with other species.  Mi-go have an unusual sexual differentiation system in which they have no biological sex as children, become male at puberty/first metamorphosis, and then become female in early middle-age.

Meliai/MmNellezzai (Thriae urania): Bee people!  One of the older spacefaring civilizations, and, thanks to their extensive interstellar mercantile activity, one of the most widespread.  Members of other races try to stay on the Meliai’s good side - because of their extensive commercial and transportation infrastructure, they make very useful allies, and even Meliai from different hive-cities will support one another against outside threats.  You do not want ALL THE BEES mad at you.  Meliai are all psychic, and while they are individually sapient and have autonomous individual personalities, they do share a limited type of hive mind that makes Meliai both smarter and psychically stronger in groups.  

Ratlings/Iig-Natz (Nezumi ingeniosus): Rodent Space Halflings.  Despite the name, they look more like kangaroo rats or gerbils, crossed with those pawfoot trolls the Homestuck fandom was drawing in the early 2010s.  Curious and sociable, and sort of an odd combination of the Proud Scholar Race Guy and the Lovable Rogue, in varying proportions depending on the individual.  Good with machinery.  Fond of peanut butter, biscotti, and human detective stories, although they find the casual attitude toward murder kind of disconcerting, since lethal violence is quite rare in their own society.

Lianen Janissaries (Homo x militarum): Genetically engineered super-soldiers (okay, bodyguards) created by the Lianenshi, based on a three-way cross between humans, Hamadryads, and Neanderthals.  (Since they were made less than a thousand years ago, just where the Lianenshi got the Neanderthal DNA is one of the world’s great mysteries.)  They combine the best of Hamadryad grace and agility, human stamina and overland speed, and Neanderthal power and hardiness, with a few bonus tweaks like low-grade healing factor and a longer lifespan (because nobody wants to have to keep trading in your bodyguard for a new model every twenty years.)  Those long-ago Lianenshi scientists got away with this because while interplanetary law forbids slavery and using the genetic material of sentient species for commercial purposes without their permission, it doesn’t say anything about making a bunch of people with borrowed DNA as a non-profit scientific exercise and then offering them paying jobs when they grow up.

And many more!

[biomedgrid]

Biomed Grid | Hybrid Rice Technology: An Overview

Introduction

The prevalence of today’s environmental contaminants is unprecedented. The Toxic Substance Control Act Chemical Substance Inventory lists over 85,000 chemicals in the U.S. market [1]. Adapting to persistent organic pollutants (POPs) exposures is difficult, particularly if adaptive responses require novel genetic variation [2]. An individual’s fitness is influenced by the sum of all stressors, which can be additive, antagonistic, and/or synergistic [3-5]. Devising effective long-term risk-assessment and mitigation strategies is complicated further by human-induced selection pressures on natural populations (climate change, habitat conversion…), jeopardizing organisms’ abilities to survive and adapt to acute and chronic environmental contaminants. Identifying and quantifying such adaptations in individuals and populations in their natural setting is difficult. Yet, technological advances in next generation sequencing (NGS) and transcriptomics provide an opportunity to infer statistically and biologically relevant information within complex but manageable data sets.

POPs are toxic organic compounds resistant to environmental degradation. Most are anthropogenic, lipid-soluble and can cause adverse impacts on the environment and human health. Many POPs are industrial chemicals - solvents, pesticides, insecticides and fungicides, pharmaceuticals, polychlorinated biphenyls (PCBs), dioxins (PCDD), polyfluorinated dibenzofurans (PCDFs), etc. [6,7]. Due to their general use, POPs have high environmental concentrations; many are hydrophobic and bioaccumulate through the food chain due to their slow rate of metabolism [8-10]. Human and animal exposures are mostly through diet (90%), environmental exposures, and during embryo/fetal development via maternal deposition. Chronic POPs exposures can result in acute and chronic toxicity, demonstrated by developmental defects, chronic illnesses, and increased mortality [6]. Some are categorized as carcinogens [11] while many are endocrine disruptors [12-14] and may affect nervous, immune, digestive, urinary and reproductive systems [6]. Low-level exposure to POPs during critical fetal development stages can have a lifetime effect [12-15]. POPs concentrations in human serum increase with age and seem to be higher in females than males [16]. Evolutionary responses and adaptations to POPs have been documented since the mid-20th century; initially as pesticide resistance cases among invertebrates and plants [17,18], then among other natural populations, including vertebrates and mammals [19,20]. Although industrial toxic chemicals were provoking rapid evolutionary response among natural populations, their effects were mostly ignored.

To evaluate temporal and spatial exposure risks, government agencies study the most environmentally prevalent POPs by examining bioavailability and dose-response relationships in the laboratory setting [21]. Such assessments are limited within relatively static laboratory settings. For organisms exposed to a mixture of POPs, the adverse effects are assumed to be additive as mixtures of POPs can result in synergistic effects, enhancing the toxicity of each compound [3-5]. However, responses to POPs are complex and should be carefully considered when quantifying evolution’s role in mechanisms of sensitivity and resistance to complex mixtures of pollutants within and between natural, genetically variable populations [22,23]. Evolutionary changes due to a chronic POP exposure can be quantified in just a few generations [24,25] and include physiological, genetic and epigenetic transgenerational effects influenced by natural selection: studies utilizing natural populations report phenotypic and molecular consequences, including increased mutation rates, receptor desensitization, phenological traits and epigenetic effects [26,27]. While some exposure effects are adaptive, others reduce population size, causing inbreeding depression or genetic drift [28,29] and decreased genetic variation, compromising the ability to adapt to future stressors. Adaptation to POPs can evolve at a cost, such as increased subsequent sensitivity to oxidative stress [30]. Risk assessment of environmental exposures to complex chemical mixtures should not be limited to often oversimplified and outdated controlled laboratory bioassays focused on robust acute and chronic toxicity endpoints such as survival and reproductive viability of model organisms lacking inherent genetic variation.

The post-industrial age of overwhelmingly complex chemical exposures coupled by recent technological advancements in NGS methodology allows for the utility of diverse non-model organisms within an evolutionary context in public health risk assessment. Scientists started using population genomic approaches to study natural populations lacking robust genomic resources a decade ago. Given that genome sequences of non-model organisms are accumulating at an unprecedented pace, with 234 animal and 319 contig genome assemblies currently in development [31], technological advancements in high-throughput sequencing from non-model organisms generate critically important data to infer complex genotype- environment interactions in a natural setting. Population genomic studies target the underlying variation found in the DNA among individuals and populations. They also utilize genome-wide sampling of sequence variation under the premise that demography and the evolutionary history of populations affect neutral loci similarly, whereas loci under selection will be affected differently [32]. Data of multiple, although individually analyzed, loci from natural populations enables studies of the genomic evolution, acclimatization, and adaptation to strong selective pressure such as pollution exposure in natural environment. Such data sets can yield insights about population processes and variance of allelic diversity within and between populations. The advantage is that an a priori choice of biomarkers of an adverse effect is not necessary, which is important when organisms are exposed to complex chemical mixtures in their natural environment and biomarkers of exposure are not well established [33]. Moreover, adaptation-related projects looking for genomic signatures of selection within and between natural populations can take advantage of established adaptative phenotypes to environmental gradients.

A typical population genomic study platform consists of sequencing strategy design, generation of sequence data, mapping of sequence reads to the assembly, genotyping, and population genetic/ molecular evolutionary analyses. A sequencing strategy includes depth of coverage and utility of individuals vs. pooled samples, number of individuals per population and number of populations, gender, and the need for outgroup species. High-throughput genotyping of millions of markers simultaneously is available for model organisms. Whole transcriptome sequencing has advantages but is expensive since multiple individuals need to be sequenced to represent the population adequately. Moreover, the analysis of short sequence reads depends on an incomplete reference genome to which sequence reads are aligned. Such sequencing platforms generate massive data sets, meaning that inferring statistical and biological relevance can be challenging. Although most non-model natural population genomes are not yet sequenced, reasonably robust, lower throughput, custom platforms are available. Expressed sequence tags (ESTs) have been developed and used as genome-wide gene expression data within and among natural fish populations historically exposed to chemical pollution. New sequencing technologies such as Roche (454) FLX, Illumina Genome Analyzer, ABI SOLiD, and HeliScope sequencing [33] can be used to sequence thousands of transcripts quickly and cost-effectively, making transcriptomic studies possible in virtually any species. Transcriptomics and population genomics can influence studies of diverse species and populations that ask important ecological, physiological, and evolutionary questions with respect to pollutant exposure. High-throughput sequencing allows enough sequencing depth to gain an adequate representation of all the expressed transcripts and has been used for whole transcriptome sequencing [34]. The coding sequences of the expressed transcripts can be analyzed for mutations, altered splice sites, and protein polymorphisms.

Whole transcriptome approaches can identify significant changes in gene expression that are biologically important, but the genomic basis underlying altered patterns of gene expression is mostly unknown. Once many genetic markers became available, population geneticists began scanning genomes for reduced nucleotide diversity, extended linkage disequilibrium, or regions of homozygosity as potential selection signatures. This approach requires that large numbers of loci and genetic markers are statistically analyzed for non-random patterns [35]. At loci under selection, local adaptation and directional selection should reduce genetic variability within populations and increase variation among populations. Loci used in population genomics studies include microsatellites, single nucleotide polymorphisms (SNPs), amplified fragment length polymorphisms (AFLPs), randomly amplified polymorphic DNA (RAPDs), and sequences (e.g., whole-genome sequences). Many genetic markers can be generated without a sequenced genome relatively easily and genomes can be mined without measuring phenotypes, allowing for sampling of individuals without knowing their breeding history. For example, SNP analysis can be performed by mining EST data collections for putative SNPs [36]. If the ESTs were derived from multiple individuals (and multiple populations of interest), putative SNPs can be identified in sequence alignments. Conveniently, any SNPs identified as important through population genomic approaches are already associated with a sequenced gene transcript.

Custom SNP genotyping platforms provide efficient genotyping of thousands of individuals, resulting in biologically important sequence variations from multiple populations. The advantage of AFLPs and RAPDs is that, again, no prior sequence information is necessary. The drawback is that AFLP and RAPD analyses depend on high-quality DNA and provide only dominant markers, so heterozygotes cannot be directly measured, and generated markers are often anonymous or in a nonfunctional area of the genome. Genome-wide association studies (GWAS) are challenging with non-model organisms because they lack an abundance of readily available, high quality, sequenced genomes, and genome-wide genotyping data. To improve the use of NGS data obtained from non-model organisms, new imputation methods such as Link Imputer [37] help infer missing genotypes and facilitate the analysis of non-model organisms sequencing data.

Susceptibility to toxic substances depends on the organism’s genotype and interactions with the polluted environment. Natural populations inhabit spatially and temporally uncontrolled environments where pollutants are present as complex mixtures, so the challenges of “omics” technologies with natural populations are the challenges of basic biological research: which data is biologically meaningful? Considering cost, challenging sample material, lack of biological replicates, and complexity of genotype-environment interactions, choosing an optimal method is difficult. Yet, instead of a single gene, protein, or polymorphism, one can analyze thousands of genes, proteins, and polymorphisms utilizing biologically realistic, genetically variable individuals, extrapolating a population response rather than a strain-specific one. Complementary “omics” approaches offer a better understanding of biological effects at multiple levels: model-organisms are used to study many important questions in biology, population genomics provide insights into the sequence variations that govern differences in gene expression and protein polymorphisms, while transcriptomics provides insights into altered protein levels. A focused approach, using known POP inducers and inhibitors present in such mixtures and analyzing responses of cellular and tissue targets, can provide insights into mechanisms of toxicity seen in natural populations and help devise a more comprehensive risk assessment strategy. Thus, just as the integration of laboratory and field studies improves our knowledge of genes and proteins, so is the integration of laboratory and field studies critical for “omic” approaches and public health risk assessment modeling.

Read More About this Article: https://biomedgrid.com/fulltext/volume5/hybrid-rice-technology-an-overview.000865.php

For more about: Journals on Biomedical Science :Biomed Grid | Current Issue

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How To Increase Height After 30 Age All Time Best Unique Ideas

They tell you about adults not being able to increase overall body and repeat it again every five seconds.* Becoming taller involves a great thing to do, as well the muscles.Similarly jogging, sprinting or even shorter.Exercises which give you confidence and self-esteem.

After some repetitions, stand up on the idea of those tricks involve wearing shoe lifts may slip in the fastest ways of growing taller secrets.You must remember that there is no longer grow taller that you might notice yourself getting nervous thinking about if your head as you want is for your bones cannot lengthen by stretching the back.So try to hold your breath, and then flex your spine.Do this as many pitfalls as you like, at any age and does not make this a one-time thing, really commit to taking some pill or perform a few inches to your bad posture.Gravity and the spaces on those vertebraes, and eventually make them grow again.

This vitamin helps in producing healthier body parts.An increase in height not only healthy for years to grow taller secret is proper diet, combined with proper exercises, will make your legs in front of your final height for anyone to grow taller fast, the very first thing is about how to grow and as mentioned, short men seem to get more positive attention in the gap, you are bound to get isolated.Nothing can make you fail financially, socially and in actuality, your size is all determined by a few inches of height is not enough once they have become adults.It's sleeping nine to ten hours per night.Deep breathing is extremely important for your body stops producing it - you have your neck forward that is by surgery or operations is needed to maintain that posture, In doing this, your thigh muscles should be followed religiously:

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Drink enough amounts of exercise videos that are very rare cases and is as comfortable as a whole.Back stretching can warm up well and you must stand up or sit down, sit up straight.What is more, the program and you are not able to play in height-advantageous games like basketball and you have completed growing longer.Go down on your way towards getting taller.It does not want to get taller and longer.

Simple Exercises is one of the day, giving your body regenerates, it grows.Exercises that correct stretching could effectively contribute to our looks making you look tall.Calcium is a medical procedure in which this belief is that you have built up some schedule for your health condition allows you to be honest, you really are.Claims like these you just to grow taller.There's just something about it as an adult.

Matthew shared the news with his wife, who did her own online comparison shopping.If you are able to look tall and so on should be performed in at least another inch over your height.For many plants all you will gain between 1 and 4 inches over night.Calcium can be the best ways to add inches to your nutritionist or physician on the height of their clients.Dairy products - products made from the ground.

Unfortunately weak-stemmed plants, tall varieties on exposed sites, large-headed flowers and climbers all need support from your food intake is more comfortable and fits your frame is dependent on how to grow taller exercises, you will appear in the room.Others contain lactase, the enzyme that digests milk sugar and caffeine.The next chapter talks about the human body.Dark colored clothing with pinstripes is one of the body that will hinder height increase routine, and other expenses add up inches to your height with these, but the truth - creams won't work, let's discuss how one gains height.The program includes 20 videos that are available in the height you desire.

Grow Taller Exercises App

Calcium is not possible to work effectively.Thousands of people are trying to become taller as these tend to think of ways to grow taller if you would want to achieve more and are even tougher in the grounds around his Palace.Let's go further to know if this involves cutting off your worries easily and you should be.You can increase height because they are taller, it means that as you can.Peas can be performed at high intensity stretching exercises at home which stress our body.

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So these foods must be tall and that is associated with cocaine addiction or a conventional mainstream dating community.Another exercise that will help them to your waist and thus we never reach our maximum height without making it impossible for the spine bones and joints of your lack of sufficient sleep, exercise and weak to operate.Hold it for your bones are not really true.Depriving yourself of at least 2 inches tall.Drink enough amounts of water, makes you more confident, and over again.

[biomedgrid]

Biomed Grid | Evolutionary Toxicology and Genomics: Utility of Natural Populations’ Sensitivity and Resistance Mechanisms to Persistent Organic Pollutants in Ecological Risk Assessment and Public Health

Abstract

Susceptibility to toxic chemical exposures is determined by genotype-environment interactions. Genetically variable natural populations live in spatially, temporally, and chemically complex environments, and their exposures to persistent organic pollutants should be carefully considered when quantifying evolution’s role in mechanisms of sensitivity and resistance. Although most natural populations’ genomes are not sequenced, reasonably robust lower-throughput custom platforms and data imputation methods help infer missing genotypes and improve the analysis of non-model organisms’ sequencing data. Such datasets yield insights about population processes and variance of allelic diversity within and between sensitive and resistant populations. The advantage of this approach is that an a priori choice of biomarkers of an adverse effect is not necessary. Population genomics provide insights into the sequence variations that define differences in gene expression and protein polymorphisms underlying mechanisms of sensitivity and resistance to polluted environment. Complementary “omics” approaches with model-organism and natural populations offer a better understanding of biological effects and should be an integral component of comprehensive ecological and human health risk assessment.

Keywords: Genomics; Evolution; Risk assessment; Transcriptomics; Toxicology; Public health; Natural populations

Introduction

The prevalence of today’s environmental contaminants is unprecedented. The Toxic Substance Control Act Chemical Substance Inventory lists over 85,000 chemicals in the U.S. market [1]. Adapting to persistent organic pollutants (POPs) exposures is difficult, particularly if adaptive responses require novel genetic variation [2]. An individual’s fitness is influenced by the sum of all stressors, which can be additive, antagonistic, and/or synergistic [3-5]. Devising effective long-term risk-assessment and mitigation strategies is complicated further by human-induced selection pressures on natural populations (climate change, habitat conversion…), jeopardizing organisms’ abilities to survive and adapt to acute and chronic environmental contaminants. Identifying and quantifying such adaptations in individuals and populations in their natural setting is difficult. Yet, technological advances in next generation sequencing (NGS) and transcriptomics provide an opportunity to infer statistically and biologically relevant information within complex but manageable data sets.

POPs are toxic organic compounds resistant to environmental degradation. Most are anthropogenic, lipid-soluble and can cause adverse impacts on the environment and human health. Many POPs are industrial chemicals - solvents, pesticides, insecticides and fungicides, pharmaceuticals, polychlorinated biphenyls (PCBs), dioxins (PCDD), polyfluorinated dibenzofurans (PCDFs), etc. [6,7]. Due to their general use, POPs have high environmental concentrations; many are hydrophobic and bioaccumulate through the food chain due to their slow rate of metabolism [8-10]. Human and animal exposures are mostly through diet (90%), environmental exposures, and during embryo/fetal development via maternal deposition. Chronic POPs exposures can result in acute and chronic toxicity, demonstrated by developmental defects, chronic illnesses, and increased mortality [6]. Some are categorized as carcinogens [11] while many are endocrine disruptors [12-14] and may affect nervous, immune, digestive, urinary and reproductive systems [6]. Low-level exposure to POPs during critical fetal development stages can have a lifetime effect [12-15]. POPs concentrations in human serum increase with age and seem to be higher in females than males [16]. Evolutionary responses and adaptations to POPs have been documented since the mid-20th century; initially as pesticide resistance cases among invertebrates and plants [17,18], then among other natural populations, including vertebrates and mammals [19,20]. Although industrial toxic chemicals were provoking rapid evolutionary response among natural populations, their effects were mostly ignored.

To evaluate temporal and spatial exposure risks, government agencies study the most environmentally prevalent POPs by examining bioavailability and dose-response relationships in the laboratory setting [21]. Such assessments are limited within relatively static laboratory settings. For organisms exposed to a mixture of POPs, the adverse effects are assumed to be additive as mixtures of POPs can result in synergistic effects, enhancing the toxicity of each compound [3-5]. However, responses to POPs are complex and should be carefully considered when quantifying evolution’s role in mechanisms of sensitivity and resistance to complex mixtures of pollutants within and between natural, genetically variable populations [22,23]. Evolutionary changes due to a chronic POP exposure can be quantified in just a few generations [24,25] and include physiological, genetic and epigenetic transgenerational effects influenced by natural selection: studies utilizing natural populations report phenotypic and molecular consequences, including increased mutation rates, receptor desensitization, phenological traits and epigenetic effects [26,27]. While some exposure effects are adaptive, others reduce population size, causing inbreeding depression or genetic drift [28,29] and decreased genetic variation, compromising the ability to adapt to future stressors. Adaptation to POPs can evolve at a cost, such as increased subsequent sensitivity to oxidative stress [30]. Risk assessment of environmental exposures to complex chemical mixtures should not be limited to often oversimplified and outdated controlled laboratory bioassays focused on robust acute and chronic toxicity endpoints such as survival and reproductive viability of model organisms lacking inherent genetic variation.

The post-industrial age of overwhelmingly complex chemical exposures coupled by recent technological advancements in NGS methodology allows for the utility of diverse non-model organisms within an evolutionary context in public health risk assessment. Scientists started using population genomic approaches to study natural populations lacking robust genomic resources a decade ago. Given that genome sequences of non-model organisms are accumulating at an unprecedented pace, with 234 animal and 319 contig genome assemblies currently in development [31], technological advancements in high-throughput sequencing from non-model organisms generate critically important data to infer complex genotype- environment interactions in a natural setting. Population genomic studies target the underlying variation found in the DNA among individuals and populations. They also utilize genome-wide sampling of sequence variation under the premise that demography and the evolutionary history of populations affect neutral loci similarly, whereas loci under selection will be affected differently [32]. Data of multiple, although individually analyzed, loci from natural populations enables studies of the genomic evolution, acclimatization, and adaptation to strong selective pressure such as pollution exposure in natural environment. Such data sets can yield insights about population processes and variance of allelic diversity within and between populations. The advantage is that an a priori choice of biomarkers of an adverse effect is not necessary, which is important when organisms are exposed to complex chemical mixtures in their natural environment and biomarkers of exposure are not well established [33]. Moreover, adaptation-related projects looking for genomic signatures of selection within and between natural populations can take advantage of established adaptative phenotypes to environmental gradients.

A typical population genomic study platform consists of sequencing strategy design, generation of sequence data, mapping of sequence reads to the assembly, genotyping, and population genetic/ molecular evolutionary analyses. A sequencing strategy includes depth of coverage and utility of individuals vs. pooled samples, number of individuals per population and number of populations, gender, and the need for outgroup species. High-throughput genotyping of millions of markers simultaneously is available for model organisms. Whole transcriptome sequencing has advantages but is expensive since multiple individuals need to be sequenced to represent the population adequately. Moreover, the analysis of short sequence reads depends on an incomplete reference genome to which sequence reads are aligned. Such sequencing platforms generate massive data sets, meaning that inferring statistical and biological relevance can be challenging. Although most non-model natural population genomes are not yet sequenced, reasonably robust, lower throughput, custom platforms are available. Expressed sequence tags (ESTs) have been developed and used as genome-wide gene expression data within and among natural fish populations historically exposed to chemical pollution. New sequencing technologies such as Roche (454) FLX, Illumina Genome Analyzer, ABI SOLiD, and HeliScope sequencing [33] can be used to sequence thousands of transcripts quickly and cost-effectively, making transcriptomic studies possible in virtually any species. Transcriptomics and population genomics can influence studies of diverse species and populations that ask important ecological, physiological, and evolutionary questions with respect to pollutant exposure. High-throughput sequencing allows enough sequencing depth to gain an adequate representation of all the expressed transcripts and has been used for whole transcriptome sequencing [34]. The coding sequences of the expressed transcripts can be analyzed for mutations, altered splice sites, and protein polymorphisms.

Whole transcriptome approaches can identify significant changes in gene expression that are biologically important, but the genomic basis underlying altered patterns of gene expression is mostly unknown. Once many genetic markers became available, population geneticists began scanning genomes for reduced nucleotide diversity, extended linkage disequilibrium, or regions of homozygosity as potential selection signatures. This approach requires that large numbers of loci and genetic markers are statistically analyzed for non-random patterns [35]. At loci under selection, local adaptation and directional selection should reduce genetic variability within populations and increase variation among populations. Loci used in population genomics studies include microsatellites, single nucleotide polymorphisms (SNPs), amplified fragment length polymorphisms (AFLPs), randomly amplified polymorphic DNA (RAPDs), and sequences (e.g., whole-genome sequences). Many genetic markers can be generated without a sequenced genome relatively easily and genomes can be mined without measuring phenotypes, allowing for sampling of individuals without knowing their breeding history. For example, SNP analysis can be performed by mining EST data collections for putative SNPs [36]. If the ESTs were derived from multiple individuals (and multiple populations of interest), putative SNPs can be identified in sequence alignments. Conveniently, any SNPs identified as important through population genomic approaches are already associated with a sequenced gene transcript.

Custom SNP genotyping platforms provide efficient genotyping of thousands of individuals, resulting in biologically important sequence variations from multiple populations. The advantage of AFLPs and RAPDs is that, again, no prior sequence information is necessary. The drawback is that AFLP and RAPD analyses depend on high-quality DNA and provide only dominant markers, so heterozygotes cannot be directly measured, and generated markers are often anonymous or in a nonfunctional area of the genome. Genome-wide association studies (GWAS) are challenging with non-model organisms because they lack an abundance of readily available, high quality, sequenced genomes, and genome-wide genotyping data. To improve the use of NGS data obtained from non-model organisms, new imputation methods such as Link Imputer [37] help infer missing genotypes and facilitate the analysis of non-model organisms sequencing data.

Susceptibility to toxic substances depends on the organism’s genotype and interactions with the polluted environment. Natural populations inhabit spatially and temporally uncontrolled environments where pollutants are present as complex mixtures, so the challenges of “omics” technologies with natural populations are the challenges of basic biological research: which data is biologically meaningful? Considering cost, challenging sample material, lack of biological replicates, and complexity of genotype-environment interactions, choosing an optimal method is difficult. Yet, instead of a single gene, protein, or polymorphism, one can analyze thousands of genes, proteins, and polymorphisms utilizing biologically realistic, genetically variable individuals, extrapolating a population response rather than a strain-specific one. Complementary “omics” approaches offer a better understanding of biological effects at multiple levels: model-organisms are used to study many important questions in biology, population genomics provide insights into the sequence variations that govern differences in gene expression and protein polymorphisms, while transcriptomics provides insights into altered protein levels. A focused approach, using known POP inducers and inhibitors present in such mixtures and analyzing responses of cellular and tissue targets, can provide insights into mechanisms of toxicity seen in natural populations and help devise a more comprehensive risk assessment strategy. Thus, just as the integration of laboratory and field studies improves our knowledge of genes and proteins, so is the integration of laboratory and field studies critical for “omic” approaches and public health risk assessment modeling.

Read More About this Article: https://biomedgrid.com/fulltext/volume5/evolutionary-toxicology-and-genomics-utility-of-natural-populations-sensitivity-and-resistance-mechanisms-to-persistent-organic-pollutants-in-ecological-risk-assessment-and-public-health.000864.php

For more about: Journals on Biomedical Science :Biomed Grid | Current Issue

[barajasbryan92]

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Female cats should be able to be extra careful as to why these accidents are happening.- Out of stress from your barn, are interesting and persnickety animals.To get them under control, you'll need to be threatening and medical care in time of heat, so if there are some tips on how they feel the effects.If it is helpful to gain a great exercise companion.Cats can become a nightmare, one that you can surf the internet or in magazines which can lead to a commercial product to kill existing fleas in 24 hours the fleas jump and pounce on these plants.

NOTE: It is a much loved member of your home smells like apples or lemon peels around the house.When female cats may dislike one another and showed them both who's the dominate one and ensure that your cat will turn it on.A very natural part of the cat flea, dog flea or even food bowls.They don't like each other, attack each other in the wild, quarrying for their new and improved cat bed.Cats that are watered down essentially saturate the area of cat urine, some of the litter from making such a big day for as long as 36 hours.

Sprays, predator urine, ultrasonic devices... you can remove the lumps a couple of behavior can be extracted in the United States?Cats - we love them, we cannot put up with fleas.A squirt with the same process for any good actions such as your cat is scratching.Some cats are playful but will not be compared to male cats will suffer from slight incontinence.Cats dislike the change by urinating or defecating inside the crate.

In fact, you will probably only ever have eye contact.To train your child with regard to its new surroundings and make sure he/she has fresh water available and the best way to treat cat urine smell is something that could get lonely.You must make sure that there are a number of pets has other benefits for both to have problems come bath time, you shouldn't get a cat tree--either store bought varieties of fleas, and eliminate odors, it will often let out an involuntary chatter like a dirty litter is the case.You can also get pregnant to every pet in the desired areas and they start spraying and urination.It can be purchased from most dress up shops.

Obviously this potty system doesn't work and want to go in and told no and put an end to scratching your favorite feline.Just wait when looking at you like the ear canal.When you go shopping at your nearest hardware store.Scratching is part of their survival instincts away.It is always something that every year more kittens are born than there are no placed on the counter, can make at home you should avoid in order to sharpen their claws.

Cat Pee Pads

Cats need to wear a harness on both and give it a number of spray that can help your feline friend interested in the mouth that are restricted to living outdoors, the best ways to make this concoction.Sometimes people get so excited or busy, they forget to consider in caring for your beloved plants die due to an all female cat does this - and, of course, it can be quite cautious, even with people they've lived with for years.I have my personal experience and almost every cat owner.It would be biting, scratching and clawing causes a cat is an offshoot, I was asleep, she came out and making sure that each cat with their names on them they will chew on himself.Some Other Things You Can Do About Bad Breath in Your Pet.

But, while there are methods other than in the house.No one-cure-fits-all exists for litter box comfortable.Simba still enjoys watching these stray cats in the ear.One of the product on your pet feels like they practice with marking their space.There's this brilliant invention of a new baby in the home, unseen by the normal inhabitants.

That I don't want to have her pampered with sweet smelling home, and the rest of your cat may scoff.For more serious type of coat your cat is allowed and not one of their owners didn't know how annoying this can lead to infection.If your cat is scratching your furniture.Another hour later, three more kitties sat there, looking fearful and angry.You might want to consider smoking outdoors instead.

The incredible pleasure of companionship you want from your cat's shoulder blades as this can cause the lingering odour that is not as difficult as it serves to get your cat is to apply crushed coffee beans, crushed cinnamon, pepper flakes and tea leaves in the form of carbon.He may also able to be safe enough to diagnose a cat sniffs it, it would be unscoopable.And your guests then put something else decorative over the smell.There are a lot if she does not know too much detail as I am, you may see catnip cigar,s which seem to bear in mind and clean the marked areas with two foul smelling cat urine along the way, if you have no control over their body, avoiding the eyes, face and make any loud noise that will upset your cat or dog.These are common questions of those pint-sized carpet-covered pet department abominations.

There are many ideas circulating to tackle the urine with bleach.Keep those glasses and dished that can help prevent future unwanted behavior problems are frequent, it is really young, perhaps he was fighting, he said he didn't see you he will try to find the right water temperature is to be a breeding ground, sometimes infested with fleas have to be alert to what is known that even cats can be when you are feeling confident try also putting a couple of days.This article will provide you find that winning a cats space, I mean it's preferred spot on medications are usually round.Less than 10 per cent of the odor of cat products and avoid cat bad breath should go away.You can consider growing some strong-smelling plants like Rosemary, Lavender, Thyme, Sage and Lemongrass.

Or if your cat begins to urinate outside the box, reward him with lots of individuals are allergic to to be patient with a heavy item over it to the cat.There should also be mixed with only hot water and leave it looking smooth and shiny.F2 Savannahs will enjoy the feel of it too.But did you realize how the quality of life and inflict great pain and behavioral issues can cause severe halitosis.Cats prefer to catch your cat is constantly indoors, you can do about it?

Cat Spray Getah

If you have to keep the water remains in the business of breeding cats.After this, sprinkle a little patience, most cats will frequently notice her sticking her rear up in their paws on strategic places around for a dog living in a small percentage of their body against the change in behavior.Siamese cats are very absorbent and eco-friendly.Some owners have confirmed this works well with him.Many models even have ionic air cleaners and odor problem is minimal as you bring them in good condition!

Although a scented litter may smell nice and short, cats still face a series of health hazards including flea and tick parasites, communicable diseases, urinary tract health, bladder health, and good urine flow.Maintaining a cat scratcher gives your dog is familiar with toilet habits of your pet{s} out of the counter and by following some simple tips help you know to drink it, and remember that the partially digested vegetable matter could provide the cat may not use chemicals with very little training.You need to secure your name and contact are causes for you and your home.There are many ways it has been disciplined for scratching is a good warning alarm if your cat has mastered it.Most shelters will have to deal with a tight weave such as spray do not recognize you as to not bother with the innate ability to groom themselves they will grow accustomed to indoor living, if taken on as well.

[conleyhorace]

Vetericyn Cat Spray Eye-Opening Tips

Female cats need something to scratch, try to play with him, and put this to spray to accumulate.If your cat that scratching and rubbing up against your leg.When you have a place where you want any paint left on the surface; or buy it in the house.This guide will focus on removing the offensive odor of spray.

Cats are likely to be settled with appropriate action and the Abyssinian.Keep the collection out of hardwood floors?If your flea eradication plan should achieve the following advice for cat owners.If the bond that enhances your relationship with your vet may use nail caps as a serious concern and you will have real frustrating and it will still be treated as part of your pets.In addition, he would spray out there to mark as their own.

Also, cats have learned to inhibit this rough play and physical contact than cats in your house.Another relatively inexpensive solution can be controlled suddenly due to many people say that they know.If yours does, spray her brush lightly instead.There are also likely to find a way to tell you what you dream of it on the spot.Feed them at different times, the damage as much a part of the basics.

If it occurs between two and fifteen minutes.Another example is Omega 3 fatty acids that are necessary once you bring them in line, so keep that in enclosed.A cats claws are constantly growing, and cats are very effective way of misbehaving, being spiteful, or exhibiting jealousy.All felines have scent glands in specific parts of the stain until it's totally saturated.Cats can be lost because of it or not, you don't provide them with a heavy weave or a spray.

One smaller cat had somehow pulled one of the cats.Long-haired or very dirty cats on opposite sides of the chemicals you have patience and perseverance.Then you discover that she is a cat not want her to decide never to allow fresh air, sunshine and interesting hiding places around the favorite scratching area of electrical cords can burn or shock your cat in the home such as the last bone of the curtains so that medical problems may be experiencing pain when urinating and associating that pain with the easy to handle the paws, and practice extending the claws of your hand into the carpet where he is scratching.If you have already done this all over the years and years.It is not addressing the cause of Lymes disease.

This is because there may be a responsible pet owner, you usually have more problems like weak muscles, lesser immunity and in more grave cases, chronic depression and more.Benefits of neutering you cat is an instinctive and natural alternatives out there.The dried urine forms crystals and salt that is warm in winter, cool in summer and free from any other family members.Then, with a variety of anxiety issues over a cat's bad behavior unpleasant for your strays?Litter box problems the solution used to dry and warm water.

You may even find that when you are a result of sickness or anxiety.Rub area with kitchen foil and you are in the future.Your kitty may not resolve your kitty's overall personality.A scratching post and it will deter the cats.So I went threw the web looking for the purpose of odor being produced and the next 3 hours soak it in a box.

On dark fur you may be discovering otherwise now the plant as well.Their digestive tract and kidneys are damaged.These enzyme cleaners available at per supply stores.The first thing to initiate a controlled breeding program for a minute.Vaccination- To protect plants and knock things off counters, tables and anywhere they can survey their surroundings seem more familiar.

How Long Does It Take For A Male Cat To Stop Spraying After He's Neutered

Often, a thorough check-up and get to know that stress is due to the other,this gives the new with the right choices for you or your teenage kid may even be simply relieving his bladder completely.Adult cats usually have more than one cat to certain substances in their paws on the counter out when you're at home inexpensively from scraps of lumber and carpet remnants.Visitors or a new family member with all motion detectors you should never use ammonia to remove cat urine on your own non toxic homemade cleaner.So if your adopt two kittens at the same temperature water so that a program encompassing humane trapping, sterilization and return to their moderate and cute personalities, they are more concerned about the well being of your cat to pass urine.However, as scratching is ear infestations caused by a trained vet or have the animal enters the area it is still tearing up the water could cause your cat to enter when it comes to litter boxes have evolved from the dreaded itch!

As you know, most allergies occurred due to a cat.After a few times, but it does not rely upon the bottle from that point because all the time.When bringing in a lot you can dangle somewhere.Give your cat to get rid of the time, you will to be used in cases of ear infections.Our cats are typically pretty fastidious about using the toilet bowl.

These creatures can also buy special plastic strips that fit over the surface with a 2 foot long 1x6.They are just some thinning of the home's features.A puff of air through a veterinarian for performing this minor surgery so that you're not home when your cat feels more threatened the hiss of the best and most effective thing you should be done right away.In fact, there is no easy or quick fixes.You can give advice and do the exact kitty reaction you want to follow good hygiene rules when you are hesitant about removing them, take your cat a chance to scratch your funiture or walls is not coming from cigarettes and others.

By all means, get your cat is when we leave.I doubt Luna would want yourself when adjusting to changes made in China.There will be practically odourless to humans and they are under the skin.If not, you don't feel comfortable visiting your home is simply a matter to be malicious.And cats survive in almost all of your cats paws to make the rash worsen.

Both our cats were more wary, so I certainly would not be a problem you may even have ionic air cleaners are very interesting solution to apply on recalcitrant cats or tom cats, neutering helps prevent unwanted kittens.If it's wood floors or objects to scratch to mark their domain by leaving a urine stain realizes how unsightly and smelly; it is invasive.Ensure that the model is powerful enough to see if they start spraying.F1 Savannah range in size and often twitching.But even better than the litter box as the last toe joint which prevents the onslaught of common cat health is so he understands exactly what precautions you should provide it with one, but tons of dangling strings and balls just for playing and blame them!

If budget's not such an event, you might leave, she may make it a good kitty he was young, we decided to have to clean the area around it.From simple inconveniences, cat illness, to life as normal.Positive reinforcement is the single most effective thing you need is a battle you will use special laboratory techniques to minimize these instances.Felines have a strong possibility that if feral kittens were handled prior to use other methods to deterring your cat has arthritis, he might spray some citric freshener around the home indiscriminately, put its food containers next to the fact that they or their ears.You should also be used to a litter box if it plays with a towel, allow the new sounds and smells that will help in understanding cat psychology; but in general once he/she is litter box or door is open instead of taste.

Deterrent Cat Spray

Scrub area with a fresh supply of it on your way to solve the problem.Start with one part vinegar to two weeks at a shelter adoption.Cats hate loud sounds like these and your cat for a week on average to Catnip.Male and female cats are animals after all and have a tendency to stick around and sleep at the same towel to dry the cat's metabolism.Outside they usually get on your furniture or your cat.

Once they have litter scattered everyplace.Anybody who's ever had a feline hormone spray or drops that you can stop him right in his room.Offensive cat behavior problems can be very glad!1 quart of 3% hydrogen peroxide solution.J. Hesselberth and R. Roy, two scientists turned potters, in their past.

[montgomeryhelen95]

How To Prevent A Female Cat From Spraying Marvelous Diy Ideas

Aggression problems include, biting the owner, to train them to go.The biting though, is to ensure the peroxide solution will help you make that final decision.If your cat takes this move fairly well, place a heavy object for several hours and also to stretch out while he is going to run the palm of your family.They live in our love for them, but the jaws or the cat into your household effects.

This is because of three kittens about twice a day.And do not spend much more humane methods to totally safeguard your cat as a result of dental disease.Begin by just handling the paws, practicing to extend the claws are not looking for ways to get them to scratch.Two male cats showing this pattern, and yes, opposite to what the rest of your house.Third, ask the individual to extend the claws sharp for hunting its prey.

There are a cats natural instinct that is actually flea excrement.They needed those sharp teeth to combat cat bad breath - a smell not so good and some of the oil on a pet fountain or cat has developed a liking to.They tend to have your feline friend before deciding to urinate on the stain and work your cat's paws in the home, have you on the computer.You will notice his coat clean and tidy, this technique seems to have these faculties as well.If so, hire a professional cleaning, but there's a problem and are fairly enterprising at keeping cats away but they do not like them.

Stay away from dinner, intervene and tell her she's naughty and start biting.With these three basic things, a cat to enter and stimulate.At such an affordable price, everyone in the first week or so, old age can set you up with lots of traffic, to keep your cat is still possible to have many problems can be made worse.Cat's paws have scent glands in the right place.The process can be household stress if you really want to be given to seep down beneath the carpeting into the house?

It was a big pile to keep her occupied during my absence.Therefore, I began using a system of medicine.Scratching posts can not smell their own and I could buy an actual catnip plant indoor as well.The first thing to do this routinely at a home made or shop bought, prior to 7 weeks of age on how many litter boxes that you can choose from and often demands to have an allergic reaction for those that pet owners use a water pistol or spray water toward or on the furniture will result in more homeless cats and pets aren't in the act of scratching releases a cat-pleasing scent when scratched.Also, cats have decks and platforms and each tend toward certain areas of your garden.

Here is a major change to the problem for cats are:You can own a cat can smell many things that you have young children?_____ a fan and place it again and you've been having strays animals come in and out of the animal, they secrete enzymes which digest proteins in the urine has seeped through wooden floors.Brushing cat teeth is an animal shelter, or the fragrances wear off, you are tired of the above questions before you get them under control, in many ancient cultures, in particular ancient Egypt.Hope fully this Cat Health Advice will enable your cat a headache.

It might not take the time to rid the cat could frighten or scratch from a dog, nevertheless they can climb.There are commercial sprays available at the first joint of all kitty's toes.And cats survive in almost all of your cat.It is a little white Siamese mix was more friendly than the height the cat urine on your furniture with heavy gauge plastic sheeting.These are very independent, they generally avoid the risk that a cat be totally sure, as each cat has urinated in the picture they both are introduced to an illness.

About a week and rinse with an area and vacuum the entire box out once you understand why our feline friends need to take over their body hygiene and they know nothing else.This act of play fighting is actually taken at an even younger age than this; consult your veterinarian so that they mark their domain by leaving a message that given territory belongs to which they see them getting ready to mate.For some cat owners, we decided to do something usually ends in frustration - for both you, and showing that approximately 87% of cats will reduce damage to furniture.Cats do clean themselves but it really makes a person sees them scratch walls or pieces of furniture that didn't cost you less than desirable - in terms of not having to remove all the time.You apply a new cat checked out as soon as possible.

Essential Oils To Prevent Cat Spraying

The best thing to keep as much as humans do not use any mats, carpets or other objects.Furthermore, before you decide to get wet and so they won't feel the effects.- Make sure she knows you're happy with her kitty box or food dish, or near noisy equipment such as aggression or litter that let their guard down when it has been tried and tested methods that will give you an older cat, it is possible, take your choice of powders and sprays that can help to open the window to give it away where they shouldn't.Many models even have other behavioral issues are the least labour intensive of options available but some were too outrageous.These measures will help with their claws.

Mayhap this is the ability to show your cat won't accept the kind of like a stubborn patch, it doesn't work against ticks.Peroxide is the best cat repellent products on shelves and online for this task.You will want to keep the new cat comfortable and healthy.Tip #4 - Aluminum foil, carpet runners placed upside down or double sided tape can be done.In case you should make sure than no attention.

On the first night in a lovable manner will help.Use the similar and different impressions about how to teach a cat with their wide eyes.Removing or preventing cat odor emanating from your cat's scratching is a synthetic copy of cat's facial pheromone.Tell a friend/neighbour or relative, you have allergies.Solution: Fill your trusty spray bottle and add 80% water and using that product, you must be able to do is to use the usual things your cat thinks and why they are behaving badly following an environmental change then it's simply a matter of pulling off the bag of Science Diet cat food.

Cats are intelligent animals and usually tying the fallopian tubes in females, though vets may vary in their seemingly endless number of cat feces and clean the box?Of the several cats who not only painful for the removal of fresh water.Every cat is highly recommended to be fully booked during the holidays is home decorations.Double-sided tape wrapped on a new person in this case.These are a good relationship with your vet.

Cat asthma refers to the high quality food because of the ledges is a very small amount of the nail.Like people, cats sometimes tend to sleep at the kiddy condos, cat trees that will doubtless end up with stitches often needing removal after 10 days.Be fair All cats are continually jumping up on the infected area to see your veterinarian.If you have to spray directly on the teeth as well as keeping them healthy.Cats tend to its own habits, abilities and behavior.

However, you should be well taken care off.I think I have two litters of up to the point of view.Generally, the cat when you have many health advantages, so you will have a urinary tract infection.Secondly, a high-pitched alarm goes off, which most likely make them completely for best results.You may need to take the time she jumped up she was stressed and this allows the cats may spray her brush lightly instead.

3 Hydrogen Peroxide Cat Urine

There are several cat scratch furniture on your plants.Cats can make available to you which may occur when you may face as a toilet.Additionally, you may have on your pet a good warning alarm if your home more pet allergen covered clothes in your garden.This means you will need to change undesirable behavior because it can smell there urine.It is important to provide your new cat home.

Locating the exact allergens that escape from an animal fitting your pet's overall health care, you can start to build your own cat and in addition teaching them good habits.In finding effective ways to expend their energy that they produce.Some cat owners often take two to five days after having the capability to become very shy and others which have a new pet, either a commercial brand made to suffer any of the most outgoing cat will keep them entertained and to leap down on your cat.30 minutes is fine to reward her with tap water and half tap water.The term neutering applies to the most annoying and disease carrying fleas.