#Chloroform Extract

[irawhiti]

prepping some gay fucking tubers for various chemical tests over the next few months to see if they contain any of a wide range of toxins because there's literally no official research on this wildly invasive plant's edibility/toxicity outside of like "this plant is eaten by grazing animals, birds, and some native species with seemingly no ill effect But we haven't sequenced its compounds or anything" so i have to do it my own self -_-. (and i'm polynesian so unfortunately i'm drawn to random tubers in the ground like a moth drawn to the depths of the underworld for a glimpse of its hellfire) wish me luck i guess

[rosewind2007]

Re-reading All Systems Red (again)

I honestly had NOT spotted that MB leaves Gurathin alone at the habitat when everyone else is out on the worm attack survey trip/assessment*—I am kinda surprised?

As someone who has dived, and also worked in laboratories with potentially dangerous chemicals we always had a buddy system—so if you did a (in the lab, not on a dive) phenol chloroform, or pollen extraction—you’d always have a buddy with you

Gurathin is all alone

Which, after the whole “It calls itself Murderbot” thing? Yeah, wouldn’t have raised an eyebrow, like it says in chapter 6:

I knew Gurathin was testing me again, but if something went wrong and he got killed, I wouldn’t mind as much as I would if it was one of the others.

BUT in chapter 1 Gurathin is still the loner with the:

small, quiet smile

Who everyone seems to like…

*Volescu, Bharadwaj and Murderbot are in the crater—then Pin-Lee and Ratthi jump from the hopper with Mensah at the controls, as MB climbs into the cabin Overse and Ratthi are there, then Arada and Pin-Lee drag Volescu inside—that’s the seven of the eight PresAux members out and about, leaving Gurathin alone

[idle-daydreams]

idk if you’re doing oc-reader requests. But uh…yeah

could you do a Yandere fyodor with a reader inspired by my oc, Nera?

the gist is, she’s the guardian of the book(the one everyone wants). However, she has a deep rooted loyalty to the agency. The only thing rivaling that loyalty, would be her love for Sigma, one of the few she views as family. She’s distrustful of people, but still nice to people she knows and likes. Her ability allows her to feel the emotions of others, but she’s very sensitive to it. She usually wears mostly back formal wear, liking pants better so she can defend herself if need be

[A.N.: Sorry for being so late, I've been busy with midterms. Hope you like it!]

Rat And Mouse

Tw: Yandere, kidnapping, manipulation, mentions of murder and torture.

Nobody knew about your connection to the Book.

It was something you’d never discussed - not with the agency, despite your loyalty to them; not even with Sigma, though he’d been born of its pages. You’d kept your head down, continuing to pretend that you were just another Agency member, just an ordinary person with a near-useless Ability. It was worth more than your life if anyone found out.

And yet somehow, Fyodor did.

You cowered before him as much as you could while tied to a chair. Though the room was cold, your skin was slick with sweat, hair hanging loose around your face. Your head was still a little fuzzy, but you were certain you’d been chloroformed in your apartment.

The man sitting before you smiled serenely, one leg crossed over the other.

“Be calm, dorogaya,” he said.

You grimaced. Your ability allowed you to feel other peoples’ emotions, yet you could sense nothing from him. Not anger, nor excitement, nothing.  It was as though he wasn’t a real person, but an empty doll with blank eyes and an unsettling smile.

It was unnatural, inhuman, and utterly terrifying.

“What do you want?” you said, trying to keep your voice from quavering.

Fyodor spread out his pale fingers. “Come now, I know they do not hire idiots at your Agency. Do you believe I do not know about your connection to the Book?”

Your heart leapt to your throat. “I don’t know what you’re talking about,” you said, trying to inject as much confusion as you could into your voice.

Fyodor smiled. “How disappointing.”

He stood up. You’d never seen Fyodor Dostoevsky in person before. In photos he’d seemed unimpressive – a pale, thin man with lanky hair and a tired expression. But what those pictures had failed to capture was the malice of his demeanour, the aura of darkness that hung around him. It was almost palpable, making your flesh crawl as he came closer.

“Please,” you said. “I don’t know what you’re talking about. I’m just an innocent—”

“—secretary? Forgive me, but I do not believe you.”

You tensed as he placed a hand upon your face, expecting blinding pain before the oblivion of death overtook you. But nothing happened. His hand was ice-cold, but nothing else.

“I said to be calm,” he murmured. “I am not looking to kill you.” To your horror and confusion, he ran his hand through your hair in an almost gentle caress. “You are very beautiful. I should have noticed a piece of such monumental importance earlier.”

“I-I-” you began, “I’m not ly-”

“Ah, please do not. Sigma has already told me everything, so there is no need for you to lie any further.”

No. A leaden weight dropped in your stomach. Sigma wouldn’t have - he couldn’t have! ‘But he could,’ a treacherous voice in your head reminded you, ‘his Ability allows him to extract information from people with a touch, remember?’

You looked up to meet Fyodor’s indifferent gaze. “You’re lying.”

He shrugged. “Believe what you wish, dorogaya,” he said.

Then he turned and left the room.

You exhaled, mind racing a mile a minute. Sigma wouldn’t have betrayed you, even if he did have the ability to do so. He hated Fyodor, and had no reason to work with him ever since the Vampire Incident. But then, the only other person who’d known your secret was your ex – actually your first, and only – boyfriend, whom you hadn’t seen in several years, and whom Fyodor had no reason to contact unless he’d already suspected you of hiding something. And the only thing which would give him cause was...

‘No,’ you firmly told yourself. ‘It wasn’t Sigma. He would never betray me like that! I trust him with my life. Fyodor must have found out... some other way.’

You shook your head vehemently to convince yourself. It didn’t matter much at the moment anyway. What was more important was the fact that Fyodor would use you to find the Book. Though you were its Guardian, you possessed no supernatural link to it, only the knowledge of its location. Something that he could draw from you through torture.

You looked around. It was a small room. There was no window, no clock, no way to gauge the time. Fyodor had tied you up rather cruelly; the rope binding your wrists had been looped around your neck, so that you were forced to keep your arms bent at an unnatural angle to avoid strangling yourself. Your eyes welled with tears as the full extent of your plight hit you. Fyodor was a madman, a cruel and barbaric terrorist no matter how mildly he acted. Your ability was useless, your fighting skills non-existent - how were you going to get out of this alive?

‘Its fine,’ you told yourself. ‘The Agency will know that I’m missing. They’ll find me. I just have to hold it out till then.’

Your arms were burning, sweat beating your brow, when Fyodor returned.

“Ah, my bird has kept herself on her toes,” he said, as mildly as though he’d caught you in some trivial task. “I am glad my arrangement was not beyond your capabilities.”

“What do you want?” you said through gritted teeth. “I’m not telling you where the Book is no matter how matter what you do, so just forget about it.”

“No? That is quite the bold claim. Are you not afraid that I will hurt you?”

“It doesn’t matter.” You swallowed. “No matter how much you t-torture me or whatever. I’m not telling you anything.”

Fyodor nodded, looking only mildly disappointed. “I admire your resilience.” He reached for your hair again, running his fingers through it to straighten it out. “There is a lot about you that is to… admire.”

His words sent a chill down your spine. Fyodor movements were gentle, almost tender – more suited to a lover than to a lunatic. You tried to pull your head out of reach, only for him to pause with a raised brow.

“You do not like it?” he said. “Apologies.”

He stepped away. You followed his movements nervously as he began fiddling with the things on the sideboard. “Tell me,” he began, as there was the clink of glass, followed by the sound of pouring liquid. “Determined as you are to keep the location of the Book from me – what makes you think I do not already know?”

“What?”

You started. Fyodor turned back to you, a full wine-glass in his hand.

“Would you like something to drink?” he said, proffering the glass to you.

“No,” you said distractedly, ignoring the dryness in your throat. “What do you mean, you already know? You know where the Book is?”

Fyodor slowly took a sip of the wine as he strolled over to you. That faint, awful smile danced upon his bloodless lips as he leaned closer. “Well, we have used a page from it before,” he said softly. “Did you not wonder where and how we got it?”

Your heart skipped a beat. Your position as Guardian meant that you knew the Book’s location, but you had no way of knowing if someone had removed it from its place. If Fyodor had somehow found it…

“Ah, I have agitated you. I am sorry for that.” He held out the glass to you once again. The wine smelled enticing; it was a deep red colour, almost the rich red of blood in the dim light. Yet your stomach roiled at the sight of it. Fyodor had the Book… if Fyodor had the Book, then the world was already as good as done for.

You met his soulless eyes, and dropped your gaze to the floor.

“You’re lying,” you said miserably, trying to think of what to do next. It was your worst nightmare; to be battling wits against one of the smartest terrorists in the world.

“Why would I lie?” Fyodor casually circled behind you, tracing a finger across the rope around your neck. You braced yourself yet again, imagining his finger slicing your throat.

“If you already had the Book, you wouldn’t need me,” you said, biting back the scream that threatened to erupt from your lips. “You’d already be halfway already to your next awful scheme.”

“Oh?” He ran his fingers along the base of your neck, leaving a trail of goosebumps in their wake. “And what makes you think that you are not part of that scheme?”

‘It can’t be.’ Your heart drummed painfully against your ribs. The door before you seemed to dance, the shadows in the corners laughing at your misfortune. “You – I’m only as important as the Book. The only reason you could want me is because of the Book. There’s no other reason.”

“Before the vastness of God, everybody is insignificant. But for sinners such as us, there might be other reasons.”

Something simmered beneath your skin, a faint flicker of an emotion so dark, so twisted, it made you feel ill.

Desire.

Lust.

Obsession.

It disappeared in a flash, as though dragged back into the void.

“I will give you a chance to make your own decision,” Fyodor murmured from behind you. There was a soft swish, and the pressure on your neck and wrists slackened. You slowly pulled your hands to your lap, realizing what had happened.

“You’re letting me go?”

Fyodor stepped back into your field of vision. “I told you; I am giving you a chance to make your own decision.” He pointed at the door. “You wish to leave? Leave.”

You stared at him, alarm bells ringing in your head. “What are you planning? You’re planning something.”

He smiled that awful, soulless smile of his. “It would not do to reveal all of my secrets, yes? You must figure out some things for yourself.”

You looked from him to the door, then back. There was no point in arguing with him. Fyodor hadn’t become who he was by being honest or transparent. There wasn’t a chance in hell that this wasn’t part of some elaborate strategy – but this was also your only chance to escape.

You stood up slowly, joints stiff after the prolonged restraint. “I don’t – whatever you’re planning,” you began guardedly, as you began backing towards the door, “but you’d better drop it. I’m not so stupid as to lead you to the Book.”

“I wouldn’t expect you to be.”

“So—” You broke off as you bumped into the door. You were wasting time. Fyodor watched as you fumbled with the doorknob, making no attempts to stop you. His eyes – those empty, all-knowing eyes – chilled you to the bone. You looked away as you flung open the door, fleeing the room. There was a narrow corridor that led down a flight of stairs, but you barely paid your surroundings any heed in your desperation to leave. 

‘I have to get to the Agency,’ you thought. ‘I have to tell them about the Book. And I need to go check up on the Book, see if it is still safe. It has to be – I’d have known otherwise!’

But an awful thought hit you just as you reached the front door. What if Fyodor did have the Book?

“What is it?”

Fyodor spoke from behind you. You jumped; you hadn’t realized he’d caught up to you.

“Nothing.” You spun towards him, hand still gripping the door-knob. “I – stay away from me!”

He raised his hands in a placating gesture. “I have done nothing,” he said. “That door is unlocked; you are free to leave. Why do you not do so?”

“Because you brought me here!” Wrung by a sudden jolt of anxiety, you threw open the door. A blast of cold wind hit you; it was night, a chill wind howling through the trees. A snow-covered landscape surrounded you, a foreign horizon visible from the distance.

“Where are we?” you said with dread.

“Siberia.” Fyodor cocked his head. “I did bring you here. But even I can make mistakes sometimes, can I not? Or do you consider me incapable of fault?”

“No! But—” Your head spun. You hadn’t drunk the wine; you hadn’t ingested anything back in the room. Were you still suffering the aftereffects of having been drugged? Or had Fyodor done something to you?

“But you said that I was part of your scheme,” you said, rubbing your temple. “Don’t tell me you realized your mistake in the last five minutes. So it stands to reason that letting me go is part of your plan.”

“Is it? What if you’ve already told me what I need to know?”

“Because I haven’t told you anything!”

“Haven’t you?”

“I—” You ground your teeth. You didn’t know. You didn’t know what Fyodor wanted. But if he was simply done with you then he would have killed you, which meant that letting you go was part of his plan. He was either hoping for you to lead him to the Book, or he was somehow using you to get to the Agency. There was no way to tell unless you wrenched it out of him.

You looked at him, and felt yet again, that faint prickle of desire radiating off of him. He claimed to be a human… and humans were capable of more than just fault. They could hunger, for more than just food or water. They could desire, and be swayed by it.

“You… want me,” you said slowly.

Fyodor smiled. “The awful thing is that beauty is mysterious as well as terrible. God and the devil fight; and the battlefield is the heart of man.”

“What is that supposed to mean?”

“Is it not obvious?”

It wasn’t. The only obvious thing was that he trying to trick you, to manipulate you somehow. Whether or not Fyodor was attracted to you – and the idea made you both want to laugh and scream – his main motive was the Book and the eventual destruction of the world.

‘I can’t stand against him,’ you thought. ‘If I try to foil him directly, he’d crush me. But if I stay close to him, I may be able to get a hint of his plans, enough to take down the Decay of Angels for good. I could just pretend that I’m f-falling for him or something. So long as I don’t reveal anything about the Book, it’ll be fine.’

It was a sickening thought. The very idea of staying close to Fyodor filled you with dread; pretending to be his lover was straight-up repulsive. And the longer you stayed with him, the more you were at his mercy.

But you were a member of the Agency. And you were a Guardian of the Book. And if that meant putting your life on the line, you were willing to do it.

You took a deep breath.

“You want me,” you said, fighting down the panic clawing in your chest. “That’s why you brought me here. And you’re letting me go because—?”

“I brought you here in a fit of passion, but it is obvious that you don’t want to be with me. And I do not want a woman to submit to me by force.”

“That’s considerate of you,” you said carefully. “But I hope it isn’t a lie. You really have no interest in the Book?”

His smile widened. “Even if I swear on my life that I am not lying, would you believe me?”

‘Fair point.’ “So if I walk away, you’d just let me go?”

Fyodor gestured towards the snow-covered woods in response.

You bit your lip. “And how far are we from the nearest airport? How am I to get home?”

“Are you really asking me that?” he said, raising a brow.

“I don’t fancy travelling through the snow at night by myself. Your guidance is as good as flailing around blind.”

He inclined his head. “True. Well, we are far from the nearest city, at least a couple of days on foot. In any case you would find it difficult to go home from there — you have no documents on you, after all.” He slyly cocked his head to the side. “My private transport will be here in a couple of weeks; you are welcome to stay with me until then.”

The revulsion deepened. But you forced yourself to nod politely. “Then… I guess I have no choice but to stay.”

“A difficult decision.”

“But—”

The words stuck in your throat. How could you talk about living with a murderous psychopath like him? How could you simply state your demands as though hashing out a deal with a roommate?

Fyodor stepped up to you, leaning to whisper in your ear. “Do not worry, dorogaya,’ he said softly, his breath tickling your ear. “I will not force you in any manner. Anything you do will be entirely by your own will.”

You shivered. His presence pressed upon you like a shroud. ‘This is just a game,’ you reminded yourself. ‘A game of cat and mouse. Just play along for a while and you can bury this guy.’

“Thank you,” you said out loud. “Could you please step aside?”

Fyodor pulled away. You threw a glance over your shoulder, at the stars twinkling in the night sky. Then, squaring your shoulders, you stepped into the Rat’s Lair.

[katiajewelbox]

A close up look at DNA extraction from Medicago leaves ☘️. The small plastic tube I’m holding shows 3 distinct layers. The tube contains pulverised frozen leaf tissue mixed with CTAB buffer and chloroform. This chemical mixture causes 3 distinct layers to form in the tube based on their density. The dissolved DNA is in the upper layer (the least dense) where I can collect it and transfer it to a new tube for further purification with isopropanol and ethanol.

[whumpees]

Kyle xy whump list

Synopsis: it's a sci-fi drama about a mysterious teenage boy named Kyle, who is found wandering alone with no memory and no belly button. Taken in by the Trager family, Kyle struggles to adapt to the normal world while exhibiting extraordinary abilities, such as incredible intelligence and superhuman reflexes. As Kyle tries to uncover the truth about who he is, he encounters challenges that reveal he is far from ordinary. The show explores themes of identity, family, and the unknown.

Episodes: 43 eps (3 seasons 2006-2009)

Warning: the show was supposed to continue after season 3 but got cancelled and in the worst cliffhanger possible 🙂 but nice show overall i enjoyed it. Watch the interview where the producers talk about the ending to know where the story was supposed to go.

_______________________________

S01

Ep1: emotional over someone playing piano and cries. runs through glass, minor cuts on his face, wakes up nicole and tells her in a little shaky voice "I am leaking" aka bleeding 😂, Crying. Teary eyes

Ep2: emotional, teary eyed "looking for his family:("

Ep6: nightmare. emotional and crying

Ep7: nightmare

Ep8: thrown to the ground, put in a choke hold

Ep9: sudden headache while using his super senses. Wobbly after using his senses. Again feeling faint while using his senses, collapses and has an aggressive seizure, in the hospital. Emotional, crying

Ep10: chloroformed with a cloth and kidnapped, half awake in the car, unconscious on the ground, takes a bit to finally come to his senses. Teary eyed

_______________________________

S02

Ep1: knocked out

Ep2: headache while arguing with someone. Snd again later

Ep3: teary eyed

Ep4: falls and hurts his shoulder during extreme training, later putting cold/hot water bag on his shoulder. glassy eyes

Ep6: head pain

Ep8: head pain. And again

Ep9: inside an underground lab and breathing toxic gas, decides to go back into the lab knowing that he couldn't survive much longer in the gas, feeling weak and breathing heavily, (flashback: foss doing cpr on him) struggling to walk and leaning against the wall, makes it out but collapses on the entrance unconscious, amanda giving him cpr, wakes up

Ep11: kind of an explosion throws him away on the ground, just laying on a stretcher but is fine

Ep12: sways, dizzy, blurry vision. Again feels dizzy suddenly and falls on his knees, blurry vision, puts his hands on his head in pain. hearing everybody arguing by his super senses and gets overwhelmed and dizzy, head pain, blurry vision

Ep14: winces in pain. Strapped to a chair to extract data from his brain and told if he resisted it'd fry his brain, given medication through iv, information being extracted, resisting and shaking, scientists say "he's on the verge of cardiac arrest" but they keep going anyway. Is fine

Ep19: cut on his finger and it bleeds a little. Heals someone and then has nose bleed

Ep20: sleep walking. Nicole told him he's sleep walking because of stress and that he should make his life 'simpler' BUT literally EVERYBODY keeps asking him for help all at once he became so stressed and started being clumsy and not pay attention which is completely out of character for him, jessy asks him if he's been sleeping and that he looks worn down and that something is wrong with him, loses control over his powers, everyone got so concerned for him cuz he kept making horrendous mistakes and told him to get some rest. He'd wake up and find that he drew dozens of stuff and he didn't even realise he fell asleep. Turns out using his powers to heal that person is what took a toll on his body. Overwhelmed by sensory stuff and things are too loud for him he feels pain, holds his head in pain and uses his powers involuntarily and it causes a mini fire

Ep22: uses his powers to resuscitate jessy and says if he fails it could kill both of them, succeeds then collapses

_______________________________

S03

Ep1: electrocuted and passes out, kidnapped, half awake in the ambulance and muttering "amanda" then injected with a sedative i guess, wakes up strapped to a bed with iv on and hooked up to some machines, tries to free himself but can't rip the straps. Takes a fake bullet for someone and falls unconscious on the ground (he didn't know it was fake), woke up on the floor and some men sit him up on a chair and he's still trying to process what's going on and his vision is starting to clear

Ep2: nightmare and wakes up startled. Amanda getting kidnapped messed him up and made him so anxious about her he kepy having nightmares, she left his sight for 1 min and he freaked out and started screaming for her (her reaction to it was hilarious she got freaked out that he got freaked out and was like WHAT???!!! 😂😂😂😂). Suuuper anxious all day cuz the psychic told him something bad will happen to his soul mate

Ep3: crying

Ep4: depressed over amanda and everybody is trying to help him move on 😂. Super drunk (to forget amanda), clumsy and close to getting himself into trouble at the bar cuz of his innocence and lack of social knowledge (it was so cute "i love you" 😂😂😂😂) and blurting out all his secrets in front of everyone, swaying, helped to walk, says he feels really sick and throws up. Receives bad news and tears up

Ep5: tears up. On the verge of crying

Ep6: tries his invention and falls off of it

Ep7: teary eyed (cute)

Ep9: jessy had a nightmare and started choking him thinking he was someone else. Using his powers to resuscitate jessy and is in pain (faking)

Ep10: electrocuted, weak and struggling to walk, electrocuted again and falls on his knees. They threatened to hurt his family and WE GOT TO SEE KYLE LOSE CONTROL OVER HIS ANGER FOR THE FIRST TIME AND ACTUALLY HURT SOMEONE AND HE COULD'VE EVEN KILLED THAT PERSON IF IT WASN'T FOR THE PLOT TWIST!!!!!! "nicole had been right when she said she didn't recognise the person i had become" OMG I WANTED TO SEE MORE OF THAT this is the worst cliffhanger in history I'm devastated the show was cancelled after we finally got to see kyle lose his innocent side i wanted to see him capable of hurting people who don't deserve good. I want to see his ANGER and how living in this world will eventually turn him morally neutral and not that idealistic "good" they make all heros be.

[instruth]

An Extract Of Dementia

An extract of rich dementia

Let the bygones be bygones

Read the Book of Alzheimia’s

Step in, breathe in the Ozone

Intoxicated to the blur amnesia

Jump off the Cliff of Oblivion

Enroll in the class of extinction

Start off with forgetfulness

Revive the joy of happiness

Take a leap to the darkness

Don’t you want to remember?

Fire from snow, red from amber

Drunkenness from being sober

Falls or the White Cliffs of Dover

President from ordinary members

Sniff a doze of chloroform

Sleep on, in the dental chair

Monitored by a girl in uniform

Rub your head, count your hair

Tooth bent as worm, on golf fair

©Johnny J P Lee

13 June 2024

A Gogyoshiren Poem (20)

Photos: J. P. Album Collection

[mxwhore]

the good thing about failing so many rna extractions is that im slowly building up a resistance to chloroform

[theprinctontapes]

shuffle your on repeatr playlist and tag 10 people

i was tagged by @houndsofdoom

starkiller-bear ghost

2. assimilate-skinny puppy

3. key entry extraction 2: holly wood the cracked- cooheed and cambria

4. jenny, you're barely alive-rilo kiley

5. pet- a perfect circle

6. harvey-her's

7. cop car-mitski

8. said the spider to the fly-the paper chase

9. chloroform girl-polkadot cadaver

10. the small of your back the nape of your neck (the flood)-the paper chase

i tag: @weenhands @pomesa @faerieyuri whoever wants to do this idc

[drluciengaudet]

To say Lucien took the news of Aurelius' death poorly was putting it mildly. After an extended internal disassociation and a short clipped exchange between his slave who wouldn't, per usual, do as he requested he reassessed the situation and what and who he'd need. Hence how he ended up where and with who he was. The climate of things as they were gave him no reason to cover his tracks. Who would blame him for taking the head of magical security with him to Egypt. Furthermore he had every right. Cairo was his. Granted, he would now have a purpose other than being an insufferable reminder of failure.

Lucien had gone to Egypt in hopes to find it was all a farce, some plan he couldn't understand laid out by his former sire. Faced with the ashes of Aurelius Hathor the lines between genius and madness started to cross as much as all the unsettled and unresolved moments shared between him and the strigoi. Lucien took the strigoi's ashes and sun ring back to his lab in France along with his son, their son.

He'd cornered Cairo there and demanded the impossible. Lucien was no expert at Necromancy, but he knew its limitations and understood a vampire didn't have a spirit, that Cairo couldn't bring Aurelius back as with necromancy and couldn't allow the strigoi's spirit to posses him eternally as a medium because there was no spirit left. With magic out, Lucien turned to science. The probability was so low, but Lucien would give anything for the chance to have Aurelius back. He had to.

While his son tried to compel him to return to the castle Lucien ended up chloroforming him and strapping him into one of his hybridization machines. There was a humane way to do these kinds of things, but Lucien had never been one to be humane to his test subjects and when it came down to it, that's all Cairo was and a failed one at that. Cairo was genetically a mix of himself, Raphael, and Aurelius so technically all Lucien needed to pull out was Aurelius genetic material and he could leave the rest and Cairo alive. This could also be done by the da vinci like operation machine in setting all the designated needles in to the bones for extracting bone marrow and spinal fluid, but Lucien set to doing each with no anesthesia or numbing of any kind.

Organs weren't really needed, but was going to take them out for good measure and had cut down Cairo's chest and was bout to split open his breast plate. The scalpel was still in his hand when he felt the unmistakable presence of his current sire. The only sound the pumps of his machines extracting the life from Cairo who had finally stopped screaming making Lucien realize the roaring noise in his head was momentarily quiet. The three piece suit he wore was coated in Cairo's blood, Lucien not even bothering to put on any surgical gear. He stepped in front of the gurney as if he could keep Raph from his victim.

"You have no right to be here, De Luca." Lucien growled defensively combative pointing the scalpel at him. "This is my lab, this is my space."

@nottheangel-raphael

[icryyoumercy]

@nimblermortal

first, because these things are important nowadays, i am not a medical professional, this is not professional medical advice, the WHO has helpful information about malaria, when living or travelling in a region where malaria happens, mosquito repellent and appropriate protective gear are mandatory and non-negotiable, and so on

quinine is made from tree bark, which makes it one of the two medically useful things made from tree bark i know of, and also makes me wonder how people learn these things. were they just. walking around biting random trees. is the desire to chew on tree bark just part of what makes humans human.

it has initially been used as a muscle relaxant by the quechua people, to treat uh. random shivering? which is apparently a thing people sometimes do? which i could look into, but then i'd probably get distracted

the spaniards brought it back to europe, as they did with so many things, and because things like germ theory and microbiology and chemistry were still centuries from being discovered, what people knew of malaria was that it causes fever and thus shivering with very noticeable periodicity. and they had just been told about a thing that can stop shivering, so might as well give it a shot. if the symptoms are all you are aware of, the symptoms are all you can treat.

and for some utterly baffling reason, it turned out it didn't just help against shivering, it actually cured malaria? which. wasn't what anyone was aiming for, but gift horses and all that.

rome, being located very conveniently in a swamp, and having a rather inconvenient amount of popes and other people important to the catholic church, was perfectly located to pioneer such treatment and make a great many of the rich and powerful (and thus by advertisment of word of mouth and rumor everyone else) want some more of this marvellous drug, which made quinine (that is, the bark it's extracted from) one of peru's most important stolen goods

then, of course, a lot of fucked up colonialism happened (including in africa, because it's hard to do colonialism while dying of malaria), because europeans were unwilling to engage in things like fair and equal trade with non-europeans, we get fun medical price gouging and attempts at monopolies and general unpleasantness, and someone finally managed to isolate the exact chemical compound instead of just grinding up the bark and mixing it with something that tastes better than tree bark

and around the 1940s, malaria treatments with fewer unpleasant side effects were discovered (which i know nothing about and won't look up because adhd), and by 2006 the WHO has declared that quinine shouldn't be used as the first choise in treating malaria for a variety of reasons, including resistant strains and aforementioned side effects

also, if you're really curious about the taste, tonic water is traditionally made with quinine, and has been used as a prophylactic against malaria. once it wasn't used for that purpose any longer, though, people have decided to add less quinine and more sugar and citrus because they didn't enjoy just how incredibly bitter that stuff was. also, the FDA says you can't have more than 83 ppm of quinine per liter of tonic water, so if you wanted to treat malaria with it, you'd need to drink some ten liters per day, and if you want to use it for prevention, you'd need around 20 liters per day, at which point malaria seems like the better option

what tonic water can help with, on the other hand, is muscle cramps! not sure how much of that is the quinine and how much is the placebo effect, but at that point, we're back to readily available and comparatively harmless

either way, in the 1860's, it was one of the few actually working medical things (along with chloroform and diethyl ether for general anaesthesia, and opium for pain relief), so they will throw it at anything that has even the slightest ressemblance to periodic fever (to be fair, a number of other things they did also had the required medical effects, they just ran afoul of paracelsus's basic adage of toxicity

Alle Dinge sind Gift, und nichts ist ohne Gift; allein die Dosis macht, dass ein Ding kein Gift ist. All things are poison, and nothing is without poison; the dosage alone makes it so a thing is not a poison.

—Paracelsus, 1538

by reaching the poisonous dosage at the same or a much earlier point than the therapeutic dosage)

[iroatechnologies]

A Step-by-Step Guide to Yeast Extract Metabolite Analysis in Metabolomics

Metabolomics is a powerful field that focuses on the analysis of metabolites within biological systems, offering crucial insights into cellular processes and metabolism. Yeast, as a model organism, is widely used for metabolic studies due to its well-characterized genome and simple cultivation. In this post, we’ll walk through a detailed, step-by-step guide on how to extract and analyze metabolites from yeast extract, offering practical tips for researchers to optimize their workflow. Additionally, IORA Technologies offers cutting-edge solutions for streamlining the Yeast Extract Metabolite Workflow, ensuring accuracy and efficiency at every step.

1. Sample Preparation

Start by culturing the yeast strain of interest under optimal conditions. It’s essential to harvest cells during the appropriate growth phase (log phase is commonly used) to ensure the metabolites are representative of the active metabolic state. After harvesting, wash the yeast cells with cold water or buffer to remove any residual growth media and contaminants.

2. Metabolite Extraction

Metabolite extraction is a critical step to ensure the representative collection of metabolites. A common method involves using a mixture of organic solvents such as methanol, chloroform, and water in a ratio that effectively solubilizes both polar and non-polar metabolites. Be sure to include an internal standard to normalize the data later. Homogenize the cell pellet using methods like bead beating or sonication to ensure complete cell lysis and efficient extraction.

IORA Technologies offers advanced tools to automate and optimize your Yeast Extract Metabolite Workflow, significantly improving the reproducibility and consistency of metabolite extractions.

3. Sample Clean-Up

After extraction, the samples often require clean-up to remove proteins, lipids, and other unwanted compounds. Solid-phase extraction (SPE) or liquid-liquid extraction methods can be employed to isolate the metabolites from contaminants. This is a critical step to avoid interference during analysis.

4. Analysis by Mass Spectrometry or NMR

Once the metabolites are extracted and cleaned, they can be analyzed using techniques such as Liquid Chromatography-Mass Spectrometry (LC-MS) or Nuclear Magnetic Resonance (NMR) spectroscopy. LC-MS is often preferred for its high sensitivity and ability to identify a wide range of metabolites, while NMR offers detailed structural information. IORA Technologies provides state-of-the-art data analysis software that integrates seamlessly with these analytical techniques, ensuring that your metabolite data is processed and interpreted with precision.

5. Data Processing and Interpretation

The data obtained from metabolite analysis should be processed using software to identify and quantify the metabolites. Normalize the data using the internal standard to correct for variations in extraction efficiency and instrumental response. Statistical analysis can then be performed to draw meaningful conclusions about the metabolic state of the yeast cells.

IORA Technologies’ Yeast Extract Metabolite Workflow solutions provide comprehensive tools for data normalization and advanced statistical analysis, enabling you to gain deeper insights into metabolic patterns with confidence.

By following these steps and incorporating IORA Technologies’ innovative solutions, researchers can ensure accurate and reliable metabolomics data, leading to better insights into yeast metabolism and its applications in biotechnology, pharmaceuticals, and other fields.

[alchemyofazeroth]

Chloroform

Chloroform is an organochloride compound. As a common organic solvent, chloroform exists as a colorless, volatile liquid at room temperature. It possesses a distinctively sweet odor that is recognizable even at low concentrations. With a density greater than that of water, chloroform sinks when added to aqueous solutions. Although it is miscible with a wide range of organic solvents, chloroform exhibits very limited solubility in water.

The name "chloroform" itself is a portmanteau derived from two alchemical terms - "terchloride" meaning a tertiary chloride, and "formyle," an obsolete name for the methylylidene radical.

In the Redridge Mountains, chloroform once found use as an inhalational anesthetic for medical procedures. Its relatively high volatility allows chloroform to rapidly evaporate and mix with air, enabling it to be effectively administered to patients via inhalation. However, the risk for patient death from chloroform was found to be too high to be acceptable, and the practice has since been rendered obsolete.

Natural Occurence

Chloroform is a volatile organic compound. It is produced by a variety of natural sources in the environment. Numerous species of seaweed have been found to synthesize chloroform, likely as a byproduct of their metabolic processes. Additionally, certain fungi present in soil are hypothesized to generate chloroform, although the specific fungal species and biochemical pathways involved remain to be elucidated. Abiotic mechanisms may also play a role in the natural production of chloroform in soils, but the precise physical and chemical processes that lead to its formation are still not well understood and require further investigation.

Interestingly, in the Redridge Mountains, the Muckdweller fish possesses specialized glands capable of producing chloroform. These glands can be carefully extracted and the chloroform harvested, providing a unique biological source of this compound. The evolutionary advantages and ecological implications of chloroform production in the Muckdweller fish have yet to be fully explored.

Anaesthetic Usage

Chloroform was first used as an anesthetic. Its fast-acting sedative and analgesic properties made it an attractive option for human doctors and surgeons seeking to render patients unconscious and insensible to pain during medical procedures.

When inhaled, chloroform vapors rapidly induce a state of deep relaxation and euphoria, followed by a complete loss of consciousness within minutes. This allowed physicians to perform surgeries and other interventions that would otherwise be excruciatingly painful or impossible to tolerate.

The use of chloroform quickly spread from the Redridge Mountains throughout the Kingdom of Stormwind and beyond. However, despite its effectiveness, chloroform's reign as an anesthetic was relatively short-lived. As more experience was gained with its use, the medical community began to recognize its dangerous toxicity, especially its propensity to trigger potentially fatal heart arrhythmias without warning. This phenomenon, termed "sudden sniffer's death," along with concerns about chloroform's long-term safety, ultimately led to its abandonment in favor of ether and other anesthetic agents with more favorable risk profiles.

Nevertheless, chloroform's historic role in the development of anesthesia cannot be overstated, as it paved the way for the discovery and use of safer, more reliable drugs that have transformed the field of modern medicine.

Hostile Usage

Chloroform has a sinister history of being exploited for nefarious purposes. Its anesthetic properties have been misused by criminals to incapacitate, render unconscious, or even murder their victims.

In fiction, the trope of a villain pressing a rag doused in chloroform over a victim's nose and mouth to swiftly knock them out has become a well-known cliché. However, in reality, it is exceedingly difficult to incapacitate someone using chloroform in this manner. Inducing unconsciousness via chloroform inhalation requires a prolonged exposure of at least five minutes.

Most real-world criminal cases involving chloroform also entail the simultaneous administration of another substance, such as alcohol or tranquilizers, or the complicity of the victim. Furthermore, once a person is rendered unconscious by chloroform, a continuous dose must be carefully administered and their airway must be vigilantly maintained by supporting the chin to prevent the tongue from causing an obstruction - a delicate procedure normally requiring the expertise of a trained anesthesiologist.

That being said, I have found records in human literature of the use of chloroform on Blackrock orcs invading the Redridge Mountains in the past. In order to obtain the effect of instantaneous insensibility from the chloroform, humans would mix the chloroform with other sedative agents before applying it in covert operations against their invaders.

[ivareriksson78]

Solvent Suppliers in Chennai: A Detailed Review of Quality and Reliability

Chennai boasts a vibrant chemical industry with a range of suppliers providing high-quality solvents for industrial, laboratory, and pharmaceutical applications. The choice of the right solvent suppliers in Chennai should ensure product integrity, cost-effectiveness, and timely delivery. This article will discuss leading solvent suppliers, the range of solvents provided, and key considerations while making a purchase.

Rationale for Choosing Professional Solvent Suppliers in Chennai

Solvents are an essential ingredient in many industries, such as pharmaceuticals, textiles, paints, and coatings. Choosing a good solvent supplier ensures:

Consistent Quality: Suppliers adhere to industry standards and provide solvents of high purity.

Regulatory Compliance: Trustworthy suppliers adhere to environmental and safety regulations.

Bulk Availability: Enables the purchase of bulk orders for industrial applications.

Competitive Pricing: Offers cost-effective solutions for businesses and researchers.

On-Time Delivery: Offers timely and secure transportation facilities.

Types of Solvents Available in Chennai

Solvent suppliers in Chennai offer a versatile range of products based on the various requirements of industries. Some of the most commonly available solvents are:

Industrial Solvents: Used for manufacturing, cleaning, and chemical processing. Examples include Acetone, Toluene, and Xylene.

Pharmaceutical Solvents: High-purity solvents used in the formulation and extraction of drugs. Examples include Ethanol, Methanol, and Isopropyl Alcohol.

Laboratory Solvents: Essential for research and testing applications. Examples include Chloroform, Dichloromethane, and Hexane.

Specialty Solvents: Custom-formulated products for specific applications. Examples include Glycol Ethers and Fluorinated Solvents.

Leading Solvent Suppliers in Chennai

There are a few well-established solvent suppliers in Chennai that offer high-quality products and superior service. Some of the leading suppliers are:

Chennai Chemical Industries: A well-established supplier with a wide range of industrial and laboratory solvents.

Galaxy Chemicals: Known for providing high-purity solvents and bulk supply options.

Solvent Solutions India: A name to rely on for specialty solvents and customized blends.

Lab Chemicals Chennai: Laboratory-grade solvents for lab and pharmaceutical industry usage.

South India Chemical Company: Expert in industrial and eco-friendly solvents.

Things to Keep in Mind While Selecting a Solvent Supplier

Before buying solvents, keep the following points in mind to ensure reliability and affordability:

Quality Standards: Ensure the supplier is ISO or GMP certified.

Pricing & Bulk Discounts: Compare prices from multiple suppliers to get the best offer.

Packaging & Safety Measures: Opt for safe and secure packaging to avoid spillage or contamination.

Delivery Options: Opt for suppliers who provide timely and efficient delivery services.

Customer Support: Opt for suppliers who provide good customer support for hassle-free transactions.

Conclusion

It is vital to be careful while selecting the best solvent suppliers in Chennai, keeping in mind the quality, price, and reputation of the supplier. Whether you are looking for solvents for industrial, pharmaceutical, or research usage, Chennai has an adequate number of reliable suppliers. By choosing a reliable vendor, you can be sure about quality products, regulatory compliance, and efficient service. Choose top solvent suppliers today and make an informed decision for your chemical needs!

[instruth]

An Extract Of Dementia

An extract of rich dementia

Let the bygones be bygones

Read the Book of Alzheimia’s

Step in, breathe in the Ozone

Intoxicated to the blur amnesia

Jump off the Cliff of Oblivion

Enroll in the class of extinction

Start off with forgetfulness

Revive the joy of happiness

Take a leap to the darkness

Don’t you want to remember?

Fire from snow, red from amber

Drunkenness from being sober

Falls or the White Cliffs of Dover

President from ordinary members

Sniff a doze of chloroform

Sleep on, in the dental chair

Monitored by a girl in uniform

Rub your head, count your hair

Tooth bent as worm, on golf fair

©Johnny J P Lee

13 June 2024

A Gogyoshiren Poem (25)

Photos Credit: J. P. Album Collection