#ypd

[digitalgate02]

Ryo: HEY! You got very good friends here, Kenny boy!

Monodramon: Ryo, please stop making them feel awkward.

Ken: Please, can you stop, Ryo-san?

Daisuke: Why does he sound so super duper familiar?

Daisuke: REEEALLY familiar...

Ken: What do you mean Daisuke?! You never had met him before, right?! Or are you saying that because I said something like that when we first met at the soccer match ten years ago?!

Daisuke: Are you a voice actor, dude?

Oh hey. I got the urge to draw him again. But I accidentally changed his jacket.

Oh yeah, i'm still inserting the headcanon that Daisuke likes a CERTAIN game character and that franchise so yeah. The joke is... Well, if you know you know.

[variouscolors-old]

I need to write a bit more about Noel-kun but... If you speak French please rest assured i'm using Google Translator + researching stuff... Because his French is fake.

He's a poser, sorry folks (^・ω・^ )

(Also I totes encourage roasting him for the bad French lmao)

[gabrielsbubblegumbitch]

Google isn't talking to me so you're the only person I can ask, I'm so sorry-

Is it possible to have NPD, APD, BPD AND HPD at the same time?-

It's honestly complicated. You know, personality disorders, particularly those within the same cluster, often share multiple traits at the behavioral level, such as attention-seeking, unstable personal relationships, and violent outbursts. However, similar behaviors can stem from very different motivations. For example NPD might act aggressively to dominate their partner and maintain their ego, while someone with BPD might exhibit the same behavior out of a fear of abandonment. What should technically allow for differentiation between disorders is their core mechanisms that underlie everything going wrong. However, in my opinion, this is largely a theoretical concept because the core of the psyche is interpreted differently across psychological paradigms. That's partially what makes diagnostic procedure so difficult. Also there are for example multiple studies about "BPD in men" or "APD in woman" which only showcases that classification is not carved in stone as symptoms can differ across other categories like gender. At the group therapy I encountered very few people with a single diagnosis. In the majority of cases, it was "XPD with traits of YPD" because fitting neatly into one prototype is not easy. Also DSM V is only one of available classifications, ICD lists like 5 more specific personality disorders.

What I'm saying is that the labels we use are the flawed results of statistical cluster analysis, and it's not uncommon for someone to exhibit traits that span across multiple clusters. In cases of someone showing traits from three or more personality disorders I would just describe it as mixed personality disorder or unspecified personality disorder, ICD style.

[detectiveichijouji]

Ok, more Noel-posting.

Now that I finished reading that specific book, I'd like to elaborate a few things about Noel/Arsenemon.

First, his evoline is basically the same as Tailmon, except for a few changes: Botamon → Nyaromon → Plotmon → BlackTailmon. The peculiar change in this line is that, except for Botamon, All previous forms had green eyes.

He's a BlackTailmon who lived in a small village within the aerial city of Larc until he went to Witchelny and started studying magic. But since digimon can absorb data from the real world... This cat digimon got access to a ton of Phantom Thief genre works -- including Arsene Lupin, the patron of this genre -- and got impressed by it. The countless stories of thieves doing things like justice and having incredible and amazing heists defying the human logic...

This then made him evolve, on Christmas, into a brand new digimon called Arsenemon. And since then, Arsenemon kept following that code, but not doing things for himself and yes for the sake of recovering Digital World treasures stolen by bad guys or mean spirited Chosen Children.

He rarely uses his BlackTailmon form, but when he does he tends to call himself "Hikage" to get intel from his targets. Hikage doesn't need pronouns, so he sticks with whatever the target calls him with.

During YPD he took also the identities of Ken and Daisuke, briefly, and just to not get caught... or to help saving the kids (under Ken's brilliant plans ofc) -- Daisuke claims Arsenemon is unable to copy his mannerisms and this is why the thief couldn't get away from the 02 kids.

But just like any other digimon or human, he has flaws. He's kinda prideful and likes to have fun so... Sometimes he can be quite insensitive and make things harder. If he's going off the rails, he needs Miss Espimon's intervention immediately, because she seems to make him realize the glaring dangers coming in his direction.

Arsenemon is not that smart, he's very skilled and creative, but even with the most genius plan he tends to get fooled or caught off guard. Though, his charming nature just makes everyone ignore when he's completely an idiot (affectionate).

Still, he had not much attachment to his allies except for Miss Espimon who had been with him since a long time ago during his stay in Witchelny, until he met Hino Youta and Hikawa Mizuki (Soleil & Lune) and formed a partnership with them. And later, grew attached of Motomiya Daisuke, who offered him kindness and loyalty (despite everything, Daisuke deeply cared about Noel after all). Thanks to those three he feels like being a digimon or human doesn't matter, because there's people who sees him as their friend.

Noel Leblanc identity was created especially for this case, previously, Youta and Mizuki didn't know him by "Noel" and yes by another forms/identities. After creating this new persona, with glaring suspicious references to his namesake Arsene Lupin, he just grew extremely attached to it thanks to those three mentioned peculiar humans.

[postsofbabel]

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[auphelia]

I know tryptic soy broth (media for growing bacteria) tastes nice... but what about ypd (common media for growing yeast)??

[xythlia]

It's been ten days since anyone has seen Jhenae Cedeno and Youngstown police believe she is in danger here.

Jhenae Cedeno is a 16 year old trans girl from North Carolina that police in Youngstown, Ohio believe travelled here to meet with a stranger, possibly someone she met online. They also believe she is in danger because she has no friends, family or known associates in Ohio and nobody has seen or heard from her since July 10th.

As most people know, Ohio is a dangerous state for any LGBTQIA+ person, but especially for a black trans girl and YPD has not been putting any effort into finding Jhenae beyond simply publishing a release on facebook through their juvenile department. We have multiple digital billboards in Youngstown, Boardman, Canfield ,and Austintown that they could be putting her info on, but they haven't. We have multiple local news stations they could be sending her information to, but they aren't. We have multiple community centers in the different neighborhoods of Youngstown they could be putting her information up in, but they aren't. There are several other community sources all throughout the north side, the west side, the east side, and the south side they could be working to find out if anyone has seen her, yet they aren't.

I know posting this does very little in the grand scheme of things, and I'm currently trying to find out if there are any search efforts going on here in my city that I can volunteer with to help find her but more people also need to be aware of her case here in general because as of right now Jhenae is being let down by our cops severely when the worst possible outcomes are still on the table and I can't imagine what her family is going through.

[yanoharuhito]

ypd (yano personality disorder)

[digitalgate02]

imagine a murder mystery story with the 02 kids.

look we got a canon detective in that team.

[variouscolors-old]

Peeps, this acc is not abandoned it just that i had lost muse for almost everyone and i'm hyperfocusing on 02TB/Rui-Ukko atm.

I'm writing post-movie fanfic rn (i finished YPD -cheers-, the blog is still active tho) if you had watched the movie already check it on @beginningobserver blog or on AO3.

The only ship i care is DaiRui atm. I have no motivation to write/draw anything but Rui-Ukko + DaiRui sorry.

I feel like opening a sideblog for my Rui-Ukko muses for a while, but Various Colors (the song i re-named this acc after) is a song from Rui's POV so it would make no sense to separate him & Ukko from this blog methinks.

Still i have a deep attachment with Rui and Ukkomon so i'm not rping with doubles of them for now. Maybe Ukkomon OCs (if any), but not Rui's Ukkomon. This is the first time i get extremely wary around other peeps writing a favorite character (i usually don't mind) so please understand my wish. It might change later in the future, tho.

Besides that, i'm just lurking here from time to time to see if this dash gets active again. I don't feel like following non-digi blogs or gen multimuses tho (the few ones i follow are from friends btw) so i'm sorry in advance.

[ladygwyndolin]

Idk if you know it but if you do, where does "Be Careful, Onee-san" fall on the YPDS

hm idk that one, let me look it up rq-

[detectiveichijouji]

✨ Oldest to Youngest - YPD edition ✨

Miyako (18yo - May 30)

Mizuki/Lune (17yo - Jun 22)

Takeru (17yo - July 19)

Youta/Soleil (17yo - Aug 22)

Hikari (17yo - Sep 26)

Ken (17yo - Oct 31)

Daisuke (17yo - Dec 1)

Noel/Étoile (16yo - Jun 15)

Iori (15yo - Dec 28)

Extra chart:

Joe (21yo - Sep 6)

Yamato (20yo - Jan 23)

Sora (20yo - Feb 21)

Taichi (20yo - Mar 29)

Koushiro (19yo -May 12)

Mimi (19yo - July 29)

Miyako

Wallace (17yo - Jun 8)

Takeru

Hikari

Ken

Daisuke

Lui (16yo - Feb 28*-29)

Iori

※ Headcanon birthday for Lui on non-leap years is Feb 28 for me at least.

[postsofbabel]

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[yeastfeaster]

this whole blog is just probably gonna be for me ranting or sharing cool shit that I do in the lab!

3/6/24

today I did a plate assay to get a quick (3 point) anisotropy based binding curve for Pol alpha on fluorescein labeled template/primer DNA. a couple months ago we did a whole bunch of these with different conditions and they were working great, but now that we're back to doing them, they aren't working anymore. I just did a quick 3 point one today (0nM, 6nM, 150nM Pol alpha) to test and see if we're getting the same mP values we were getting before, and we are not. I think something is up with our plate reader cause the values were like 10x less than they should be at our previously discovered binding saturation (which started around 150nM pol). big sad for today in that regard.

Another thing I did today was restreak a yeast clone I've been growing. I transformed it a little bit ago, screened a couple colonies, and now I'm restreaking a few of the clones positive for my DNA. The DNA in question is a linearized plasmid integration, which I integrated in the middle of the yeast Trp1 operon. The DNA also has N-Terminally STREP-tagged Sen1 flanked by a Gal promoter so we can overexpress it. I tried linearizing my plasmid with 2 different restriction enzymes in the Trp1 operon this time (being SnaB1 and BSU361), because the last time we did this linearization, some weird stuff happened. our lab's yeast strain acts weird with Trp1 integrations if you linearize with BSU361, and we had done this before. The resulting protein was inactive and during the prep we could see a whole bunch of strange truncated product. We think relinearizing and reintegrating might help that.

Anyway, I'm confirming stable integration of Trp1 by streaking onto YPD (regular yeast food) plates, and then back onto -W (no tryptophan) selection plates. Our yeast can't grow on -w without Trp integration, so growing them on that plate confirms they have what I'm looking for. Additionally once I'm done restreaking I'll do another heat-pop PCR screen to check for Gal1-Sen1. if the PCR is able to amplify that region, it confirms that the plasmid got integrated into the yeast.

I'm doing a whole bunch of other stuff right now but I think im gonna provide background for that when it actually comes up. ill share some pics too! here's the first restreak of that clone onto YPD. I took a colony from each of these guys and streaked them onto -w earlier today.

Also, here's that colony screen i was talking about. most of the clones had the integration, seeing as there are bands of a size that I expected in each one (except 5s its dumb)

[snailmilksolution]

9/19/23

holy SHIT i want to leave this job so fucking bad

like this is all compounded by the fact that im so, so close to leaving this lab and that im splitting time with another lab that i like a lot more, but oh my GOD everything is so much harder than it needs to be. had to spend 30 minutes running back to my lab to pick up some YPG plates for the colonies and he caught me as I was waiting for the shuttle to come back around and asked how it was going. told him i was only there to grab plates and we spent ten genuine minutes going back and forth over the point of streaking on YPG vs YPD and for some reason i could not get through to him that it was simply a point of comparison to ensure theyre actually respiring. like oh my fucking god im not trying to run a TMRE on this thing its literally just to see that theyre capable of respiration and not solely relying on fermentation after, you know, editing the proteins that go into allowing them to fucking respire. its not that deep because it shouldnt need to be at this stage. jesus fuck. literally had to come back at 5:30 and get it ironed out.

the fact that its only tuesday and i have to spend the rest of the week there and not at the other lab makes me want to throw up for real. i already know the presentation tomorrow is going to go poorly and im probably going to sob in my car about it. and even then itll only be wednesday. cant even pretend to have covid to get out of a week of work because only now am i actually responsible for things and i need to turn shit out at the speed of fucking light. i know that isnt his fault at least but god i could have used some of this energy back in like june and july when i was just sitting on my hands.

at least the tagging worked. baby's first western went well and honestly was pretty fun.

[moleculardepot]

YPD Agar Medium

YPD Agar Medium Catalog number: B2014072 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 250 g Molecular Weight or Concentration: N/A Supplied as: Powder Applications: molecular tool for various biochemical applications Storage: 2-8°C Keywords: Yeast Extract Peptone Dextrose Agar, YEPD Agar Grade: Biotechnology grade. All products are highly pure. All solutions are made with…