#vanguard frisk

[niyanoireee]

vanguard frisk heart emoji

reblogs appreciated kissy emoji

[captainstrawberrywings]

Damn it Morgana you told Ren to go to sleep now he sleeping in battle

[legomocfodder]

Vanguard Squadron from Star Wars Squadrons

Keo Venzee, Frisk, Lindon Javes, Grace Sienar, and Gunny

I know Squadrons isn't really relevant or played much anymore, but that's never stopped me from making minifigs before

[itzjoseph04]

There's my Xtale Oc:

Name: X-Joseph Dreemurr

Soul: Determination

Rank: Great Admiral

Race: Human

Headcanon VA: JP:Yūichi Nakamura (same va of Gojo); EN:Philiph Reich (Same VA of Giorno Giovanna); ITA:Renato Novara (Same of luffy)

Story: Joseph was initially a orphan who at the age of 6 the Dreemurr family adopted him, at the start he gave great raport with his brother Asriel, instead of frisk and Chara, who they saw him like a "Fourth wheel" of the family, but time later they accept to live with him.

He joined the royal guard, at age of 16, and at 18, with permission of his father, he took half of the new recluses of Royal guards and found the navy part of Royal guards, named "New Advanced Vanguards of Loyalty" aka "Navy".

Now at age of 19 he's the top rank of the Navy, and top of him there just the rulers, his parents, and under at him, there are the Three admirals (Yes, is a one piece inspiration), whose role is to protect the people and the royal family

[chaoticlatexknight]

ULTRAMagic Alternate Character Rules

Given how there is mature art for UMAE, I am going lay some ground rules.

Rule of Thumb: If I haven't drawn a character and they are not appropriate for mature art, they won't appear here on this blog and I will be not drawing mature art of them... FOR OBVIOUS REASONS.

Rules:

List of Characters:

Some things to note:

These changes are only retroactive to characters on the SFW only list. Unless I SPECIFICALLY ask you to take down art, you are fine.

If a character in my gallery is not listed here, it is most likely a retired character. Please do no draw them until I decide to use them again (my discretion). Note that this mainly applies to older characters. Some characters may not be ready to be placed on the list yet. This applies to newer characters. And I may have missed a character or two (let me know if that is the case)

I will not change my stance on the SFW characters, so don't even bother asking. I also reserve the right to move characters into the SFW category at my discretion.

Unless I specifically asked you take down art made prior to the list, assume you're fine

There is a lot of characters on display here, but just remember the following: This list covers multiple series, many characters are important to other characters, and there is overlap with younger and alternate versions of certain characters

Some of these characters will only ever be mentioned

All characters can be found in their respective folders.

Yes, I am fully aware this can potentially spoil future characters. And YES, I even alphabetized this for easy reading and reference. Also this will be updated as time goes on.

SFW Only characters:

Aoife Crawford/ULTRAMagic Devil

Bethany Briggs

Brendan (child + wolf)

Charles Blackwell Ford

Cynassa (young) (UMAE)

Dark lord Kris

Deadlock/Zas (young)

Dr. Ethan Luminate

Elaine "Ember" Gabriella O'Nessie

Eldritch Frisk

Gabriella Pari

Grendel Bombastus Scarfe

Leonardo Hammond O'Nessie

Orion

Randalph Theoprastus Scarfe

Stolas (young)

Terrus, Toa of Shadow

Trevor

ULTRAMagic Ex

ULTRAMagic Revenant

Will (child)

Zelgo (Pre-reboot)

Verdict: These characters are either too young, not human, are legitimately not into NSFW, or are a unique situation. If I find NSFW art of these characters, I will expect you to take it down ASAP.

Please refrain from NSFW/Mature art of:

Buster Ash

Brendan (adult)

Captain Roger

Cynassa (UMAE)

Ellen the Wayward

Ignatius "Infinity" Daren Ford

Kyu #9

Lucifuge Rofocale (UMAE)

Olivia Briggs

Stolas (UMAE)

ULTRAMagic Walker

Vladislav Velimir Faust

Verdict: I am not saying no, but I'm also not saying yes. Please refrain from drawing NSFW/Mature art of these characters.

NSFW/Mature art wouldn't make sense for:

Abraham Van Helsing (UMAE)

Adrien Irons

Af, Angel of Anger (UMAE)

Antares Briggs

Archangel Raguel (UMAE)

Auda

Baphomet (UMAE)

Barna Schindewolf

Chernobog (UMAE)

Dark Queen Dunja (UMAE)

Desislav Robles

Dr. Adalph Wolfgang Faust

Dragoslava the Indomitable

Empress Eliza-Rex

Englehart Schindewolf

Gilgamesh, King of Uruk (UMAE)

Heinrik Rofocale

Hemah, Angel of Rage (UMAE)

Hermes Trismegistus/Nicolas Flamel (UMAE)

Lady Blood Rose of Grishnakh

Lapsus Spiritus Fausta

Loki (UMAE)

LBRX - HALVAR

Marin Schindewolf

Mercurius II

Monsterlock

Professor Marcel Matheo Germaine

Quetzalcoatl (UMAE)

Set (UMAE)

Sir Odo, Knight of the Unlight

Spiritus Magni Antonia

Spiritus Magni Athena

Spiritus Magni Drusa

Spiritus Magni Octavia

Spiritus Magni Vita

Sun Wukong (UMAE)

Thaddeus

Trumna Wintergate

ULTRAMagic Blood Wraith

ULTRAMagic Boulder

ULTRAMagic Daemon

ULTRAMagic Desperado

ULTRAMagic Disciple

ULTRAMagic Dreadnaught

ULTRAMagic Entropy

ULTRAMagic Frost

ULTRAMagic Hunter

ULTRAMagic Lance

ULTRAMagic Magistrate

ULTRAMagic Master Skull

ULTRAMagic Reaper

ULTRAMagic Thunder

ULTRAMagic Vanguard

ULTRAMagic Ultimatum

Unlight Will

Victor Von Frankenstein (UMAE)

Vlad III Dracula (UMAE)

Weaver Craddock

Will (Red Lantern)

William "Tusk" Willfort/ William Owyn Von Hohenheim

Zal-Rint

Zoltan Tenebre Raphael Dracul

Verdict: NSFW/Mature Art would not make much sense for these characters. Some reasons are as follows:

Some are well beyond carnal desires

Some have no interest in that stuff

Some are evil and are not to be trifled with

Constructs and Robots have no interest

Eliza-Rex has no time for that nonsense

Some characters are emotionally unstable

TL;DR You better have a GOOD REASON for why you're drawing this kind of art for these characters.

Characters I'm neutral on:

Abezithibod (UMAE)

Cynassa (Adult) (UMAE)

Dark Queen Dunja (Floralia Alternate)

Durlexxy "Lexxy" Golumms

Farrow

Faustus

Haunt

Mira

Samantha

Spook

Will (Absol/Adult)

Will (Carnage/Adult)

Will (Inque/Adult)

Will (ULTRAMagic Alternate/Adult)

Verdict: I don't mind with these characters, just be mindful of who they are, what they're like, and what setting they come from.

Acceptable NSFW/Mature characters:

Alfonso Devious

Antonio Pari (+ Spiderlock)

Celena

Cheryl Garnier

Deadlock/Zas (Adult)

Dolus & Iocus

Erika Storm

Hel Jessica Ford

Iden Athenon

Lerissa Bloodfang

Marion "Tanya" Elizabeth Devilfay

Maya Athenon

Max (Eloslime)

Morrigan Devilfay

Ms. Phantasm

Ornias of Aquarius (UMAE)

Stark Devious

Turvy-Topsy (Adult)

Valentina Pari

Will (Floralia Alternate/Adult)

Will (Phantasm LLC/Adult)

Will (Gorgon/Adult)

Will (LD/Adult)

Zelgo (Reboot)

Verdict: These characters are acceptable for NSFW/Mature art. They are all adults and appear as adults. They are either my characters or characters I have permission to use. In regards to characters I have permission for, check with the owner regardless before drawing.

[pyreflydust]

Top 250 tags 6-27-2023

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to the abandoned sacred beasts

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rebecca streisand

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food

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story: restless

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[niyanoireee]

OHMY GOD IM SCTEAMJNG RN

also the reason you can’t find anything about them is bc it’s a custom made au ; it was formed by me and my friends SOO LMFO. IDK WHERE U FOUND A BATTLE SPRITE FOR THEM? BUT I LOVE THIS SO MUHHC KISSES AND LOVES TO U XOXOXOXO

New drawing after I'm not even sure how long

I've researched this character for 30 minutes and i can only find a battle sprite and 3 posts by @niyanoireee so credit to them.

[darth-memes]

HAPPY MAY THE 4TH!!!

[cosmik-homo]

I feel like I'm nearing the end of the star wars squadrons story mode and it's making me so sad... I'll miss my vanguard squadron buddies, y'know? Fighting is fun in multiplayer too but I need my found family ok

[fatehbaz]

The perfect storm had been building for some time. Ferguson is at the bottom of the income spectrum and has acted as a sort of vanguard for the outward march of suburban poverty. [...] [T]he dwindling population, fleeing industry, and plummeting property values had created a budgetary crisis, forcing many of the area’s small municipalities to rely less on their shrinking tax base and more on extra-tax fees and fines, enforced by the police and facilitated by the city’s arcane court system.

The result was that Ferguson and similar suburbs existed in what the Huffington Post called “a totalizing police regime beyond any of Kafka’s ghastliest nightmares.” Out of a population of roughly 21,000, over 16,000 Ferguson residents had arrest warrants issued. And this number only counts individuals with warrants, not the total number of warrants. In 2013 this figure was a staggering 35,975, roughly 1.5 warrants per person in the city.

These warrants were part of a complex racket designed to impose unrelenting fines on the poor population in order to fund the city government, which itself had largely been redesigned to facilitate this predatory practice.

In 2013 fines, court fees, and other such extortions accounted for some 20 percent of the city’s budget. These fines were disproportionately applied to the city’s black residents, with black drivers twice as likely to be stopped, searched, and arrested as their white counterparts. [...]

---

These funding systems are not unique to St Louis, but instead became a national trend as more and more municipalities found themselves in dire conditions after the last crisis. The suburbanization of poverty and skyrocketing incarceration rates have thus been paired with growth in these massive, extra-tax extortions applied to the poor -- and particularly the suburban and rural poor, who are more likely to live in small, cash-strapped municipalities (or counties) with a dwindling tax base and less access to federal aid. In most places, this takes the form of an expanding net of legal search, supervision, and harassment that essentially extends the walls of the prison out into the new suburban ghetto.

Increasingly expensive incarceration is gradually replaced by a predatory probation system composed of extra-carceral monitoring, fines, and seizure of property, all amplified by the fusion of public budgets and for-profit probation companies. 

Many of these are relatively recent trends, with Ferguson’s dependence on probation funding skyrocketing after 2010. But rather than an unfortunate exception, Ferguson is a window into the future. As low growth, deepening crisis, and general austerity continue [...] [t]hese cities will be forced to find new sources of funding, and the easiest way to do this is for better-off residents to utilize existing legal resources in order to prey on the poor.

As the economic situation becomes increasingly dire, similar patterns emerge at greater scales: the county, the state, and the federal government will all turn to such predatory practices, facilitated by growing armies of police and preexisting legal mechanisms for debt collection, surveillance, and incarceration.

---

These patterns are piloted in the poorest areas, applied first to the most disadvantaged social groups. In Anaheim, California, the poorer, predominantly Latino neighborhoods in the city have seen a series of gang injunctions, allowing plainclothes police to arrest and open fire on residents for things as simple as their clothing color or gathering in a crowd. In 2012 a sequence of police shootings in the city led to nights of rioting just outside D!sneyl@nd. In the poorer parts of New York, stop and frisk policies and the enforcement of laws against minor offences (such as selling loose cigarettes) have allowed for similar practices, resulting in local riots around the killing of Kimani Gray in Flatbush in 2013 and national riots around the killing of Eric Garner in 2014. Similar practices have long been applied to the rural poor, including the black residents of regions such as the Mississippi River Delta, Native residents of reservations such as Pine Ridge, Latino farmworkers across the country, and the white poor in places like the coal-mining towns of Appalachia.

-------

Text by: Phil Neel. Hinterland: America’s New Landscape of Class and Conflict. 2018. [Bold emphasis and some paragraph breaks/contractions added by me.]

[dissociativedaydream]

I'll Come Back to You

Pairing: Feresk Tssat "Frisk" x gn!reader

Word Count: ~1.5K

Tags: Mostly fluff, little bit of reader being a worrywart, tiny bit of licking lol

A/N: I don't know too much about the character other than what I found on the wiki and recounts of people who played the game 😅 hopefully it's not too OC - also sorry it's kind of short but I thought the ending was cute so I'm sticking by it.

“Hey if it isn’t my favorite bartender!”

You sigh, there weren’t too many regulars around these parts but one Trandoshan was determined to get your attention every time.

“Hi Frisk, the usual?” You put on your best customer service smile, flinging the towel you were cleaning the bar with over your shoulder.

“You know me so well.” He chuckled as he plopped himself down with a noticeable sigh in his usual spot by the bar well.

Weird. No flirty compliments or trademark smirk on his face.

“Bad day?” You asked, placing a glass of Trandoshan ale in front of him.

His eyes flickered across your face briefly while he nodded in thanks before glancing down at the drink, his face not betraying anything.

You backed away, kind of surprised by his mood. For the last several months he’d been coming here it’d been nothing but flirting and compliments, he always seemed to be in an upbeat mood despite his work with the Vanguard Squad.

More patrons walked in and you moved to serve them but kept stealing glances over at the lumbering man. His mood didn’t seem to change as the night wore on, you were careful to keep his glass full. Eventually, the bar quieted down and you could stop and pry.

“Did I do something to deserve the quiet treatment?” You teased, leaning over the bar a little bit away from him, not wanting to crowd him too much.

“Not a thing sweetheart,” he replied, not looking up from his drink.

You waited a beat, not sure how to proceed or if you even should. You decided against it, instead opting to move down the bar doing some cleaning. After a little bit, you had the feeling someone was watching you.

“Do you have a story for me Frisk?” You asked, peeking over your shoulder in time to see him drop his gaze back down to the counter.

He started tapping his claws against the bar for a moment before putting some credits down. “Not today dear, I just wanted to see your face before I leave.”

“What's going on?” You wouldn’t normally question a bar patron like that but you couldn’t stop yourself. The Trandoshan turned to look at you seemingly surprised at your question.

You laughed nervously, wondering what the hell was wrong with you. He chuckled in response, “I’ll be back sweetheart, I’ve got a mission to do.”

Your heart dropped a little bit. He was worried about a mission? From the stories he told you were a little surprised. He’d always been so confident in his flying skills and for a guy with the Imperial death mark on his head, he always seemed so carefree.

“You’re not getting soft on him are you?” Your coworker came behind you, causing you to nearly jump out of your skin.

“Don’t scare me like that!”

They smirked, “that sounds an awful lot like deflecting. So you are getting soft on him! I knew you would!”

“No, I’m not! Why would you think that?” You turned away, worried that they’d somehow sense the heat that was currently radiating from your face.

They snorted at your statement, “right, well. No one is blaming you, if he dates the way he flirts he’d probably worship the ground you walk on.”

You let out a breathy laugh, trying your best to return to your work but your mind wandered to the albino pilot and whether you’d see him again.

~

It had been weeks since you last saw Frisk. While that wasn’t unusual you couldn’t help but jump to conclusions and think the worst. You almost hoped he died in a dog fight. You didn’t even want to entertain the thought of him being caught by the Empire. Bad enough he had been in trouble for the stupid counterfeit painting with the Imperial officer, but now also aligned with the rebels?

You groaned, leaning back in your seat. You were supposed to be doing the budget for your supervisor but you couldn’t focus. This was stupid. Since when were you worried about Frisk? It wasn’t that long ago you were complaining about his long-winded (and probably embellished) stories and his insistence that you be the one to hear them all. But now you’d kill to hear one. Even if it was another of his famous repeats.

Groaning again, before dropping your head onto the desk. The musty smell of old flimsi filling your nose. Stupid old fashion owner wanting to use this stupid old book when a datapad would be so much faster. You wanted to get back out to the bar instead of sitting here alone with your thoughts.

A familiar voice drifted into the office from the bar, interrupting your thoughts but you could only catch your coworker’s response.

“Sorry, she’s working in the back tonight. Can I get you anything?”

You sat up straight suddenly, there was only one person that would ask for you.

“Yeah, a Trandoshan ale please.”

You stood up fast enough to hit your leg on the low desk, mumbling a quiet swear you didn’t stop, you had to confirm that you weren’t dreaming.

Sure enough, sitting by the bar well was your favorite pilot. He was drumming his talons against the bar like he was nervous watching the other patrons at the bar. His eyes wandered before falling on you standing in the doorway, his body posture perking up.

Your feet moved before your brain could catch up.

“Aren’t you supposed to be doing the budget?” Your coworker asked, a knowing grin on their face.

“Yeah, yeah, yeah.” You mumbled in response, eyes focused on the man in front of you.

He chuckled as you threw your arms around his neck, his own arms going around your waist.

“Now that’s not the welcome home I was expecting but I’m not complaining.” His breath tickled your ear, his body warm against yours.

“I thought you weren’t coming back.” You murmured back, burying your face in the crook of his neck.

You inhaled deeply, he smelled like sweat and something metallic, he must’ve come straight here after getting back. His hands on your back started to trace patterns and he nuzzled into your neck.

“I’m enjoying this sweetheart but pretty sure everyone at the bar is staring at us.” He relaxed his grip, giving you a chance to move away.

Instead, you pulled him tighter to you. “I don’t care. I was worried about you.”

“Sweetheart,” Frisk released your waist and grabbed your arms to position you for him to see your face. “Seriously. You don’t need to worry about me. I’ve made it this long.”

You glared at him. “You were acting weird before you left. I assumed you were worried about your mission. You’re never worried.”

He sighed before chuckling again, “I’m a damn good pilot. You don’t need to worry your pretty little head about me.”

You made a face at him, after months of hearing him talk and getting to know him you could tell he was holding something back.

“It was a little more dangerous than the usual mission,” he relented. “But nothing the Vanguard Squad couldn’t handle.”

“Don’t scare me like that again.” You dropped your voice down to a whisper as your coworker approached, drink in hand.

Frisk’s eyes softened, “I honestly didn’t think you’d care.”

You smiled, “You came in here regularly, telling me all those stories and flirting but didn’t expect anything to come of it? Really?”

You had to stifle a giggle as his eyes lit up. He pulled you closer, bumping his forehead against yours. His comlink started beeping, an uncharacteristic growl coming from him.

“I’ll be right back, it’s probably the general.” He mumbled before moving to the far side of the bar, giving you a wink as he answered it.

“Aww look at you.” Your coworker finally interjected with a smirk on their face. “Finally admitting to your lil crush.”

You rolled your eyes with a smirk, “Yeah, you got me. Guess I should probably finish that budget.”

“Nah, I think you’re not feeling too well. You’re all feverish and loopy, acting weird and stuff, you should probably go home.” They grinned before leaning in, dropping their voice to a whisper. “I’m not going to say if it’s with anybody.”

“You’re a dork and I appreciate you.” You whispered back, returning your attention to the Trandoshan at the back of the bar.

Frisk notices your shift in attention and winks again, curling a finger to beckon you over. As you walked closer you could hear fragments of the conversation, it sounded like they were talking about the next mission.

“Got it, I’ll be there first thing in the morning. Goodnight General Syndulla.”

“Bright and early start tomorrow?” You asked.

An arm curled around your waist, effectively pulling you into his lap. Nuzzling your neck, he doesn’t answer right away, seemingly enjoying your closeness.

“Frisk?” You prompted.

He sighed against your skin before briefly trailing his tongue along part of your exposed collarbone, causing your breath to get caught in your throat.

“Some of the Imps got away on the mission, the general wants us to see if we can find them.” He murmured before nuzzling into the crook of your neck again.

“Is it dangerous?” You asked, your brain honestly not working too well with the Trandoshan’s hot breath against your neck.

“It’s war, my dear, it’s all dangerous. But I’ll come back to you, I promise.”

[niyanoireee]

Since the main monsters mentioned previously dont sound too far off from canon, is there one that -is- significantly different presonality wise?

Also if chara is fed up with frisk, how does frisk feel about it? Or feel about chara overall?

And i get the feeling the fanart implies that they share a soul and/or determination, is that so?

Thnx for replying btw

if you didn’t alr know, just for context this au is formed by multiple people.

nope they don’t have much that differs from canon except the clear outfit changes and character development. Cause it’s rlly just undertale 7 years in the future with a fucked up timeline.

Let’s see, if there’s someone i had to name changed personality wise it’s flowey. He’s not the same as canon; far off. He’s kinda like in a way depressed? He appeared like once early in the story to try and get Frisk to reset and give up. That didn’t work and he’s kinda turned into this like shadow. He used to follow Frisk around because of interest, but now his whole existence is like miserable. He doesn’t have reset save and load anymore, he isn’t the power he once was. And now here he is being tormented by this fucking timeline. So yea, character who’s changed the most is prolly flowey.

Frisk didn’t find out what Chara was tryna do yet. But she’s well aware that Chara doesn’t like her. Frisk doesn’t hateee Chara, but she holds a slight resentment for him because he’s a reminder. Reminder of her grim past just like Flowey. Plus; Chara is very moody and does not hide the fact he detests Frisk. He’s always snakily remarking some insult in her ear whenever she does an “impure” action. Frisk overall just tries to ignore Chara because she doesn’t see any reason to hate him. If anything, Frisk is also annoyed with Chara because he’s such a hypocrite. He had literally been the one to lead her on the genocide when she first began,, LITERALLY THE ONE SUPPORTING HER. But look at them now, now they’ve switched up and are putting all fingers on Frisk. Frisk feels like he’s constantly egging on her despite his own wrongdoings. So it’s not a mutual dislike, more like; Frisk wants to make things right and Chara couldn’t care less for what she wants to do. Frisk tries to reason with Chara like very often, to try and look for his advice so he feels like he has a choice and isn’t just a ghost. Because well, she’s the only one who can see him. Chara doesn’t give actual advice; He’s more like a “dumbass just figure it was out” type of guy. Anyways, I’ll definitely ask the creator of Frisk for more info so you can get an actual rant :3 (no i didnt create Frisk, i made the design for them. I was the one who made Chara tho!!)

And, no they don’t share a soul or DT. Chara is looking to leech off that determination so he can have a soul of his own and ERASE the timeline. Chara is only able to be there because he’s leeching off Frisk’s determination. If he wasn’t doing that, he’d also wouldn’t have been around so often. But, little fun fact!’ His locket has a tiny bit of DT that he stole from Frisk. If you look at the arts i think you can see the red at the bottom, or like he’s holding it when she’s looking o forgot.

thanks for asking 🫶

[stephenjaymorrisblog]

Today’s Pig is Tomorrow’s Bacon

Thursday, June 11, 2020

 By Stephen Jay Morris

©Scientific Morality

       History to some is a boring subject.  The way it was presented to me in grade school was all about memorizing dates and peoples’ names you didn’t really care about.  It was about the mid term, and then the final exam.  Public school curriculum was always a tug of wag between progressive methods and conservative techniques of education.

So, who won the war?  Neither ideology won.  It became a compromise of mediocrity.  Most subjects in school were anemic because of censorship by various groups on the left or right; mostly right wing Christian groups.  That is why science classes were so flimsy.  You couldn’t teach that the world was billions of years old because it contradicted the bible.

History is not boring! You remember your own name and birth date, don’t you?  Well…I am going to lay down some history on you: my own!  My history parallels social history.  Back then I wasn’t just a spectator of history, but a participant.

In the late 60s, what was happening in the black community was a daily ritual of police brutality. If you were black and walking anywhere, you were stopped, frisked, searched, and asked for identification. Were you to show any disrespect to the cops, you would “accidentally” fall down on your face and cause injuries to your body.  It was their word against yours.

Other non-white people suffered the same injustices by the police.  And, so did white hippies!  Long hair meant you were on dope and you were a “nigger-lover.”  I had my share of police harassment.  My long hair covered my face, so my white skin privilege was suspended until I got a haircut.  “Are you a boy or a girl?” a cop would ask.  There was also penis privilege, of which mine was revoked because I had long hair.  There used to be heterosexual privilege; mine was canceled until I got…well, you know. If you had long hair, the cops would call you a cock sucking queer.  All I had to do was get a haircut and all my problems would be over.  However, for those who were Black, it was a constant, day-to-day struggle.

Enter the Black Panther Party.  The Panthers were not some ideological or political group.  In the beginning, they were a militant Civil Rights group, which ultimately became the vanguard of the New Left revolution.  They supported Third World resistance and uprising against Imperialism. They were in support of the Viet Cong and others.  Their focus was on police brutality.  The Panthers went on street patrols, armed with rifles and law books.  When they saw cops harassing someone, they would stand nearby, shouting instructions to the victim on how he should legally respond. They put a halt to police harassment for a couple of years.  It was illegal to call a cop a curse word, so the Panthers would call them “pigs.” That word was and is protected by the First Amendment of the Bill of Rights.  Once, I used that word to some cops.

In 1970, I was on my way home, walking.  I was in the alley that ran adjacent to my house, when some unmarked cop car stopped beside me.  Inside, there were two plain-clothes, white cops in dark sunglasses, with sideburns and mustaches. They looked like characters from the sit-com, “Reno 911.”  They were dressed in casual clothes, like male models out of a department store ad in the Sunday paper.  The driver asked me, “Have you seen a Chicano wearing khaki pants and a yellow shirt, about 6 feet 4, walk by here?”  Now, I was a stupid, 16-year-old militant, so in a reflex action, I replied, “I didn’t see shit, pig!”  Both of them were clearly exasperated, plus they were wielding giant flashlights that cops commonly used as Billy clubs.  They slowly exited their car and approached me.  One of them asked softly, “What did you say?”  I felt a surge of adrenaline invading my belly; I knew that I was going to get the shit beat out of me!  Just then, behind me, I heard my mom open the front door of my house. With great relief, I turned around and said, “Hey, mom!”  She didn’t say a word, but she looked concerned.  The head pig said to my mom, “Ma’am!  Tell your son to watch his mouth!”  They both got in their car and sped off in pursuit of their suspect. What was even scarier was that this incident occurred right next to my house on Martel Avenue!

That’s just one of many encounters I had with the cops.  This next anecdote took place in my high school history classroom.  My history teacher enjoyed holding class discussions of current events.  One day, were talking about police brutality and how to stop it.  Now, this was 1969 and the network news nightly showed film clips of cops bashing and whacking the hell out of student demonstrators with their batons.  By this time, I was in solidarity with the Black Panther Party, who’d proposed having community control of the police department.  I said to classroom, “We should abolish the police department!”  One girl’s eyes popped out her head and she shrieked, “Are you out of your mind?!  Who is going to protect the public from crime?”  To this, I replied, “The community peace patrol, I imagine!” Then she had the nerve to recite this bumper sticker text, “Well...if you don’t like the police, next time you are trouble, call a hippie!”  It turned out that her dad was a cop.  The bell rang and my teacher said, “What Stephen said is a valid point.  Good day.”  Wow!  No teacher had ever validated anything I said!

So here we are.  It seems a million years since the Chicago cops assassinated Black Panther Fred Hampton, and it’s been just 15 days since a cop murdered George Floyd.  His murder started a wild fire of protests and riots around the world.  There are calls now from the Black Lives Matter movement to defund the police.  I also heard some anarchists calling for the abolition of the police department.  Bam!   Some ideas never die.

So, what do I believe now? Since the police are part of the working class, they should be revolutionized toward progressive values. They should take off the uniform and not carry guns.  They should be trained in the martial arts.  They should be peace officers not police officers.  Progressive unions should represent them.  I say abolish the police department and transform it into a community peace force.

The white-wingers (formerly, right-wingers) claim they are pro-police.  They are not.  They only support the cops when they violently abuse some poor victim.   Now, should the cops go on strike for higher wages, you will not see one conservative on the picket line with them. Conservatives are not willing to pay higher taxes to support their local police.  Hell, no!  They’d rather privatize the police force and have them guard and protect the white rich and their acres upon acres of property.  Here’s one argument worth contemplating:  Abolish the police so they can never be privatized!

Okay, Boomer?  

 Take a listen to this song I wrote in 1980 about the Los Angeles Police Department:

https://www.youtube.com/watch?v=LpIzWoS0Vto

[thepnictogenwing]

brief reflections on music, being bad with music, and being bound up with music

we have a curse of sorts, as a plural system: music.

in our “real life” childhood (as opposed to all the other childhoods we’ve experienced), we developed a great love of classical music very early‚ fortified by a school field trip to the concert hall in San Diego to see a performance. we devoured library books about composers and musical instruments. we daydreamed about becoming a composer or a concert violinist or even a conductor.

in vain. we lack a good sense of left-right coördination; we do very poorly in tasks that required concerted effort with both hands, and that rules out most musical instruments. our attempt to learn violin met humiliating failure at the hands of a sarcastic 6th grade music teacher, and while we made a couple attempts later in life to learn a musical instrument—trying piano lessons and guitar lessons—nothing ever “took”, and eventually we gave up the dream as dead. the entire subject was becoming too painful to contemplate.

we’ve also never really learned about musical structure, composition, or anything of that sort. possibly we don’t have the brain for it, or maybe our traumas hinder us—in any case, the subject has remained opaque to us.

so it’s been profoundly ironic that our system has come to depend so heavily upon music. part of our substance, you might say, includes the pieces of music that help define our characters: “Undertale” music, “Deltarune” music, “Fate/Zero” and “Fate/Apocrypha” music, many other pieces of music. we listen to this music, we try to attune ourselves to it—and yet, we don’t really know how to talk about it. it’s not like film or literature; we can explain (roughly) why we respond to some films and stories, but not others. we can’t do that with music.

it also seems quite fitting that I’m the vanguard of the “Undertale” presence in this system; I introjected well before anyone else, and I have been by far the most active UT fictive introject in the Pnictogen Wing...and yet, I have no theme music. Azzy does, Undyne does, other do; even our introject of Frisk considers “Ruins” to be more or less theirs.

not me, though. Toby Fox gave me nothing. I exist as a hole in the game, a cypher, and that goes for the game’s music too.

~Chara of Pnictogen

[biomedgrid]

Biomed Grid | The Nucleocapsid Protein Gene as Excellent target for Detection of Canine Distemper Virus by Reverse Transcriptase-Polymerase Chain Reaction

Introduction

The Canine Distemper Virus (CDV) belongs to the genus Morbillivirus of the Paramixoviridae family, Mononegavirales order and is a pleomorphic virus with a lipid envelope, a diameter of 150 to 300 nm, whose genome is a single-stranded RNA, with a negative sense and with a helical nucleocapsid.

The genome consists of about 15.7 kilobases (kb), which codes for six structural proteins: the nucleocapsid protein (N), the phosphoprotein (P), the matrix protein (M), the polymerase (L) and the envelope glycoproteins, the fusion protein (F) and the haemagglutinin (H). These last two, located in the lipid envelope surrounding the virion, are responsible for the recognition and entry of the virus into the host cell [1-4]. The helical nucleocapsid contains the N, P and L proteins, which initiate intracellular replication. The M protein connects the glycoproteins of the surface and nucleocapsid during viral maturation [1,5].

The N gene, of around 1.5 kb, is one of the most conserved genes of the CDV genome and it has highly conserved regions in the first 2/3 of the gene between members of the genus Morbillivirus and encodes the most abundant of the structural viral proteins, the nucleocapsid protein, which has regulatory functions of transcription and replication, as well as the encapsidation of the RNA genome in a resistant RNAsa nucleocapsid. However, despite being a highly conserved region of the CDV genome, some variations of the N gene have been shown among field isolates [6-9].

Diagnosis of CDV. Of the diagnostic methods, the clinical signology is the main tool used by veterinarians, however, the non-specificity of the signs in some cases makes it impossible to reach a final diagnosis, so it is necessary to use laboratory tests. Among them, one of the most used is the ELISA (Enzyme-Linked Immune Sorbent Assay), which can detect serum antibodies (Abs) IgM (against the nucleocapsid proteins, N and P of the CDV) or IgG (against the antigens of the envelope, H and F of the CDV). However, this test does not differentiate if the Abs produced correspond to maternal Abs, vaccine or infection. Otherwise, the production of Abs depends on the stage of the disease and may not be in the initial and final phases of it. In dogs not vaccinated with acute infection, they can die without presenting Abs against the virus. In animals with neurological signs, the determination of specific Abs against CDV in cerebrospinal fluid not contaminated with blood, is a definitive diagnosis of DC encephalitis, however this is a very invasive method and its results are variable [7,10].

Another diagnostic method used is the detection of eosinophilic intracytoplasmic inclusion bodies by cytology or by immunofluorescence, however, these can only be visualized in certain periods of the disease, generally being absent when clinical signs are generated [10,11]. Immunohistochemistry is used to detect viral antigens and/or inclusion bodies in tissues, with reliable results only when there is a marked viremia, and this technique can be performed exclusively on samples taken postmortem [7,9]. The isolation of the virus has a very high sensitivity and specificity, however it is a laborious and very slow procedure, so it is not routinely used for diagnosis [11]. The RT-PCR consists of an exponential amplification of DNA fragments of the virus, after reverse transcription from RNA to complementary DNA, allowing the presence of the virus RNA to be detected early, being a Unlike the other techniques mentioned, a sensitive, specific and rapid test for the diagnosis of CDV can be positive even when other tests fail to detect the virus [7,10,11]. In the last decades, several methods based on RT-PCR for the diagnosis of CDV have been developed, being the main targets for amplification, genomic regions that present a high degree of conservation among the virus isolates. This is how, as in 1999, Frisk et al. developed a method, based on the amplification by RT-PCR of a conserved 287 bp fragment of the N gene, which was later used by several authors, proving to be a very effective technique for the detection of the virus genome [7,8,12]. The main objective of this title memory is to diagnose CDV molecularly, through the implementation of the RT-PCR assay of the nucleocapsid protein gene.

Materials-and-Methods

This work was carried out in the Virology and Microbiology laboratories of the Department of Animal Preventive Medicine of the Faculty of Veterinary and Animal Sciences of the University of Chile, with financing from FIV PROJECT 121014019102010

Controls

Positive controls: For positive vaccinal controls, blood was used from the negative control without vaccination, contaminated with live attenuated CDV virus obtained from the vaccines “Nobivac® Puppy DP” (Onderstepoort strain), “Canigen MHA2PPi/L” (Lederle strain) and “Vanguard” Plus 5/CV-L “(Snyder Hill strain). For this, each vaccine was reconstituted with 0.5 mL of 0.9% sodium chloride, and then this mixture was added to 0.5 mL of blood, to later extract the RNA. The ten positive controls from national isolates of the virus corresponded to viral RNA. These isolates came from domestic dogs from Santiago de Chile and were confirmed as positive by a previous RT-PCR based on the hemagglutinin gene [13,14]. Negative controls: Blood was used with anticoagulant of two uninfected animals, of an adult dog without clinical signs of the disease, without a risk and without vaccination history and of an adult dog without clinical signs of the disease and without a risk history, but with a calendar of daily vaccination (vaccinated for the last time ten months ago).

Suspicious samples of DC

Eleven samples of peripheral blood were analyzed with anticoagulant (2 mL), coming from dogs that showed nervous signs compatible with DC and that in some cases presented positive to the ELISA test specific for CDV, with IgM antibody titers ≥1: 80. These samples were collected from veterinary clinics of Santiago de Chile, maintained in refrigeration at 4 °C and processed in less than two weeks. In addition, they were classified according to race, sex, age, vaccination status against CDV and anti-CDV IgM antibody titers (Table 1 )

Table 1: Classification of peripheral blood samples from dogs with suspected nervous signs of CD, according to: race, sex, age (y=year; m=month), vaccination status against CDV and anti-CDV IgM antibody titers.

Obtaining the phlogistic layer

In blood samples with anticoagulant, negative controls and animals suspected of DC, the preparation “Histopaque®-1077” from Sigma-Aldrich® was used according to the manufacturer’s instructions, for the isolation of lymphocytes and other mononuclear cells, which are the target of the CDV infection, so a higher concentration of viral RNA was obtained in the samples where the virus was found. For this, 2 mL of Histopaque®-1077 were placed in centrifuge tubes, adding 2 mL of blood with anticoagulant, and then centrifuging at 400xg for 30 minutes at room temperature (RT). The mononuclear band (opaque interface) was then carefully transferred with a Pasteur pipette to a clean centrifuge tube, washed with 10 mL of saline, shaken and centrifuged at 250xg for 10 minutes, to then aspirate. The supernatant and discard it.

Viral RNA extraction using “Trizol LS” kit from Invitrogen®

750 μL of Trizol reagent was added to the mononuclear blood cells, obtained as described above and to 250 μL of the positive vaccine controls (blood contaminated with vaccine), incubating for five minutes at RT. Then, 200 μL of chloroform was added to each tube, they were mixed vigorously for fifteen seconds and incubated at room temperature for five minutes. Then, they were centrifuged at 7000xg for fifteen minutes and the aqueous phase was transferred to a clean tube. For the precipitation of the RNA, 0.5 mL of isopropanol was added, left at room temperature for ten minutes, centrifuged at 7000xg for ten minutes, the supernatant was removed, washed three times with 75% ethanol (1 mL), fifteen were shaken seconds in vortex and centrifuged at 2000xg for five minutes. For the resuspension of the RNA the supernatant was removed, the RNA precipitate was dried under vacuum for five minutes and resuspended in 100 μL of nuclease-free water. Finally, the RNA was incubated at 55-60° C for ten minutes and kept at -20 ° C for later use.

RT-PCR

A 96-well, 96-well Apollo thermocycler (CLP, USA) and a protocol with appropriate temperatures, times and cycles for each stage were used. The primers for the RTPCR were sent to be synthesized to the Bioscan® company, their sequences being in a highly conserved region of the nucleocapsid gene: P1: 5’-ACAGGATTGCTGAGGACCTAT-3’ and P2: 5’-CAAGATAACCATGTACGGTGC-3’. These primers allowed to amplify a fragment of 290 base pairs (bp).

RT-PCR reaction

This was done using the “SuperScript ™ one step RTPCR with platinum Taq” kit (Invitrogen®) according to the manufacturer’s instructions. The reaction began with the synthesis of complementary DNA and a pre-denaturation performed in one cycle (45°C for thirty minutes and then two minutes at 94°C). The PCR amplification was carried out in forty cycles, which consisted of a denaturation (94°C for one minute), alignment of the starters (60°C for two minutes), elongation (72°C for two minutes), and a final extension in one cycle (72°C for ten minutes).

Detection of the DNA fragment synthesized in the RTPCR

The RT-PCR products were analyzed by means of 2% agarose gel electrophoresis in Tris-HC1 buffer (100 mM Tris-HC1, 10 mM EDTA) and comparison against a molecular size standard: Hyperladder IV (Bioline®). The PCR product was mixed with a commercial loading product (Fermentas®) and then the electrophoresis was carried out at 90 V for forty-five minutes. After electrophoresis the gel was incubated with ethidium bromide (0.5 μg/mL) for thirty minutes, then visualized in an ultraviolet light transilluminator and subsequently photographed.

Sequencing of Amplified Fragments

The fragments of DNA obtained from two positive samples were sent to be sequenced, to the Sequencing Center of Genytec Ltda

Bioinformatic analysis of the nucleotide sequences of DNA fragments

The sequences obtained were aligned using the free online program Clustal Ω for obtaining a consensus sequence for each sample used. In addition, the consensus sequences were entered the BLAST program to identify the origin of the DNA fragments obtained in the RT-PCR.

Analysis of results

Those samples that after RT-PCR synthesized a DNA fragment of approximately 290 bp were considered positive and after analysis with the BLAST program their nucleotide identity corresponded to CDV.

ResultsImplementation of the RT-PCR assay of the CDV Nucleocapsid Protein Gene

Once the RT-PCR protocol of the nucleocapsid protein gene of the CDV was established, the operation of the method was tested with positive and negative controls, to later analyze the field samples suspected of DC

Controls

Figure 1: Visualization of products amplified by RT-PCR by electrophoresis in 2% agarose gel and subsequent incubation in ethidium bromide. Lanes 1 and 2 correspond to the negative controls, unvaccinated dog vaccinated and without vaccination respectively. Lanes 3, 4 and 5 correspond to positive vaccinal controls, negative control blood without vaccination contaminated with Onderstepoort, Lederle and Snyder Hill vaccine strains, respectively. Lanes 6 and 16 correspond to the molecular size marker (100-1000 bp). Lanes 7, 8, 9, 11, 12, 13, 14, 15, 17 and 18 correspond to the positive controls of viral RNA from national isolates to CDV.

Three positive controls from commercial vaccines, ten positive controls corresponding to CDV RNA (national isolates) and two negative controls from uninfected dogs were processed. All these controls were subjected to the RT-PCR of the nucleocapsid gene of the CDV and after the visualization of the products, all the positive controls generated intense bands of around 290 bp and in the case of the negative controls, they did not present visible bands (Figure 1 ).

Suspicious samples of DC

Eleven samples of peripheral blood of dogs with nervous signs concordant with DC were collected and, in some cases, they were positive to the specific ELISA test for CDV (IgM) (Table 1 ). All these samples were submitted to the RT-PCR of the nucleocapsid gene of the CDV and after visualization of the products, ten samples (canine 1 to canine 10, Table 1 ) presented bands of around 290 bp, the expected DNA fragment. On the contrary, the sample from canine 11 did not generate visible bands (Figure 2 ).

Controls

Figure 2: Visualization of amplicons by RT-PCR by electrophoresis in 2% agarose gel and subsequent incubation in ethidium bromide. Lane 1 are the positive vaccine control, Onderstepoort strain. Lane 2 is the negative control, uninfected dog without vaccination. Lane 9 and 16 are the molecular size marker (100-1000 bp). Lanes 3, 4, 5, 6, 7, 8, 11, 12, 13 and 14 are blood samples from canines 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 respectively. Lane 15 are canine 11 (Table 1 ).

Sequencing of amplified fragments and bioinformatic analysis of the nucleotide sequences of the DNA fragments

The DNA fragments from two samples considered positive (canine 1 and canine 3, Table 1 ), were sent to be sequenced in triplicate to the Sequencing Center of Genytec Ltd., obtaining five sequences for each sample. The selection criteria for these samples was based on the ages of the animals, where the six-year-old canine 1 was chosen, which is the age where chronic encephalitis would most frequently occur due to CDV infection, which could be related to greater nucleotide variations according to [7]. Likewise, dog 3, eleven months old, was chosen, which is the age in which more animals are usually infected by CDV (less than one year old). The sequences were then aligned using the Clustal Ω program, obtaining a consensus sequence for each sample used: from canine 1 the sequence CDV/CMC1 was obtained and from canine 3 the sequence CDV/CMC2 (Annex 1) was obtained. Next, the consensus sequences were entered into the BLAST program, to identify the origin of the DNA fragments obtained in the RT-PCR, which revealed that the sequence CDV/CMC1 presented a percentage of nucleotide identity of 95% and the CDV/CMC2 sequence of 94%, with respect to the first one hundred results corresponding to CDV, and that in the case of both sequences, these corresponded to CDV (Annex 2).

Discussion

The CD is a disease of worldwide distribution, viral and highly contagious, affecting a wide range of terrestrial carnivores and some marine mammals, which have had a progressive increase in recent years, infecting even animals with their vaccination plans at day. It is due to this increase in hosts, that a true diagnosis of the disease caused by the CDV is crucial, to limit the spread of it to other animals and to give an opportune treatment to the sick. Although the diagnosis of CD is based mainly on the clinical signs of the patient, in certain occasions, it is difficult to reach a final diagnosis in this way, since the main respiratory and digestive signs are common to other diseases that affect the canines and in the case of the more specific clinical signs of DC, such as nervous signs, these most commonly occur in the later stages of the infection, after it has become more widespread. Also, the different clinical presentations of DC vary from one animal to another, making the final diagnosis more complex [1,10]. Although there are several techniques to help the diagnosis of CD, such as virus isolation, immunofluorescence and ELISA, most of these methods are laborious, time-consuming and may give false results, which is why they are convenient for the definitive ante-mortem diagnosis of the disease. For this reason, a sensitive, specific and rapid method is necessary to detect a small amount of the virus early in the infection, which, thanks to the development of molecular biological techniques such as RTPCR, may be possible, where a good white sequence is required. conserved between different strains of the CDV, to avoid mismatches of the splitters and the subsequent failure to amplify it.

In this work a diagnostic method for CDV was implemented through RT-PCR, based on the detection of the nucleocapsid protein gene, a highly conserved region of the CDV genome, which makes this an excellent target gene for the molecular detection of this virus. In fact, several authors have revealed the suitability of molecular biology methods based on the nucleocapsid protein gene for the detection of CDV [1,6-8,12,15]. Additionally, the primers used in this study are in the most conserved region of the nucleocapsid protein gene, the central zone, which would increase the sensitivity of the method [7,8].

Although two Faculty reports based on the CDV RT-PCR have been carried out in our Faculty [13,14], these were based on the hemagglutinin gene, which has the highest antigenic and genetic variation among the CDV genes, presenting about 10% variability between different strains of CDV, which is not the most suitable gene for the diagnosis of the virus [8,11].

In the present study, the previous observations of the RT-PCR utility of the CDV nucleocapsid protein gene were confirmed, with the virus RNA found in about 91% (10/11) of the field samples suspected of DC (Figure 2 ). This result suggests that the protocol used in this title memory, from the obtaining of the phlogistic layer to the visualization of the amplified products, could be highly sensitive. At the same time, this method would be more sensitive than the protocols implemented in previous studies, since [13] only found viral RNA in 7% (3/42) of the samples analyzed that came from dogs suspected of DC and in the case of [13], he found the RNA of the virus in 83% (5/6) of the samples analyzed, which had previously been confirmed as positive by another RT-PCR, however in twenty field samples of dogs suspected of DC, did not find viral RNA [13]. However, to corroborate the sensitivity of the method implemented, additional studies are required. The specificity of the method was corroborated with the amplification of the expected fragment in 100% (10/10) of the positive controls and in the nonamplification of the fragment expected in the negative controls (Figure 1 ). As for the negative controls, it is important to note that one of the animals used for these controls, had its vaccination schedule up to date and had been vaccinated for the last time ten months ago, which supports the preliminary observations that a previous vaccination does not causes false-positive results [7].

On the other hand, CDV can remain active indefinitely at -70°C, -192°C (liquid nitrogen) or lyophilized and only remains active for about a month at -10°C [16], without However, in the positive controls corresponding to CDV RNA (coming from national isolates), it is important to note that this RNA had been stored for more than a year at -20 °C, which is not the ideal temperature to maintain integrity and not degradation, but despite this, the stability of the virus RNA was not affected. Regarding the type of sample chosen for the field samples, blood was chosen with anticoagulant (ethylenediaminetetraacetic acid “EDTA”), because it is one of the most suitable as a substrate for RT-PCR, since it is an easy sample of obtain, maintain cell morphology for a longer time preventing the viral RNA from being degraded by endogenous RNAses when leaving the cell and also the amount of EDTA present in the tubes to obtain blood, would not inhibit the result of the RT-PCR [17]. However, similar results have also been seen with serum and urine samples, further increasing the sensitivity of detection if more than one is used for the diagnosis of the disease [1,7].

Until now, confirmation of CDV infection in live dogs was not useful, mainly due to the low level of sensitivity of the available methods, such as the ELISA test, where the finding of neutralizing antibodies in general does not correlate with the results of the RT-PCR [7], as in this study, where some animals positive for CDV infection had lower antibody titers than those considered positive (<1:80); which could indicate the non-contributory role of neutralizing antibody titers for the etiological diagnosis of the disease.

The field sample suspected of DC that was negative after being analyzed by the proposed method (canine 11, Table 1 ), could have been due to the absence of viral RNA, since either because the virus was not in blood, it could be in other epithelia of the body or because the sample was poorly preserved or poorly processed.

Although DC disease is less frequent in developed countries due to immunization, in recent years, the virus that produces it has emerged as a significant pathogen of vaccinated animal populations, and in the case of this study, the presence of some animals suffering from DC, which were at least once vaccinated against the virus, which raises the hypothesis of failure in the immunization of the vaccine, which could be due to incorrect vaccination plans or genetic variations of the virus [6], however, in order to respond to these presumptions it is necessary to carry out other studies on the subject.

Annexx 3:

After obtaining the sequences of two of the samples that amplified a fragment of approximately 290 bp and defining the consensus sequence for each of them (Annex 1), the BLAST program was used, where it was obtained that both sequences presented a percentage of nucleotide identity greater than 94% with respect to the first one hundred results corresponding to CDV and that in both cases the amplified fragments belonged to this virus, corroborating the specificity of the method implemented (Annex 2).

Annexx 4:

The technique implemented, would allow the early isolation of infected animals and the establishment of an appropriate treatment. In addition, in the case of wild animals, the rapid and specific diagnosis given by this method could help the conservation of protected species, which have been at risk due to the dramatic increase in CDV hosts occurred in recent years.

Conclusion

The implemented system represents a fast and specific antemortem diagnosis method of the Canine Distemper disease, being effective for the detection of the virus. The stability of the RNA of the Canine Distemper Virus was not altered when kept for more than one year at -20 °C.

According to the genomic analysis of the sequences CDV/ CMC1 and CDV/CMC2, both showed greater similarity to the Onderstepoort strain of the Canine Distemper Virus and, on the other hand, both sequences showed greater similarity to the American 1 lineage of the Canine Distemper Virus

Based on the results of this study, the implemented method can collaborate with the prevention and control of the increase in Canine Distemper, since it provides a rapid and specific diagnosis for the identification of the responsible virus, which could help improve health of the dog population, as well as that of other animals that are susceptible to the disease.

Read More About this Article: https://biomedgrid.com/fulltext/volume5/the-nucleocapsid-protein-gene-as-excellent-target-for-detection-of-canine-distemper-virus-by-reverse-transcriptase-polymerase-chain-reaction.000935.php

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[ruffsficstuffplace]

The Viridian Vanguard (Part 2)

Note: There’s gonna be a lotta blood and violence in this chapter, what with no Aura keeping things relatively clean in this universe.

The boundaries and barriers were set, Blake and Zwei inspected the arena for anything out of the ordinary, Ren and Nora sat at at a commentary box set up on a tree, Penny recording and powering a Fae version of a speaker system strung up around the grounds.

The crowds talked excitedly among themselves as Blake and Zwei escorted both Winter and Qrow into the arena and under the commentary box, where they were sniffed, frisked, and had the fit of their armour and helmets checked, the sharpness and strength of their swords tested.

They quieted down as Ren stood up. <Is the arena ready?> he asked.

<The arena is ready.> Blake replied, Zwei nodding with her.

<Are the combatants properly equipped?>

<The combatants are properly equipped.>

<Are the spotters ready?>

<The spotters are ready.>

Ren nodded. <Proceed to your duties.>

Blake and Zwei bowed or made a respectful gesture, before they went to opposite sides of the arena, stopped just outside the ring of shield generators and rope.

Nora stood up, she and Ren began to address Qrow and Winter, one in Actaeon, the other in Nivean.

“Do any of you wish to step down?”

“We do not.” Qrow and Winter replied.

”Are the combatants ready to fight?”

“We are ready to fight.”

“Are you all aware of the rules?”

“We are aware of the rules.”

“Then prepare for combat.”

Qrow and Winter cast each other surly looks, before they went their separate ways, stopped at an equal distance from each other.

Nora and Ren addressed the audience now. “This duel will be Tooth and Claw! No magic, no martial arts, only whatever the combatants naturally have, whatever is presently in the arena, and the equipment prescribed to them!

“Victory shall only be won by death or surrender, nothing else!”

The crowds were starting to whip themselves into a frenzy again, Qrow and Winter unsheathed their weapons and got into combat stances, Weiss tensed up and clutched the fabric of her skirt as Nora put her lips to the ceremonial horn.

Its bellow echoed throughout the training grounds, Qrow and Winter charged!

Ironbark struck ironbark, the strikes so fast Weiss could only see the reflection of the light off the swords. Punches were thrown; blades, limbs, and other body parts were grappled; kicks were aimed anywhere and everywhere, especially between legs. Sword points and edges were driven towards heads, hearts, and anywhere else important and/or vulnerable.

The two disengaged, they eyed each other carefully as they began to circle each other. They only ended up with superficial damage to their armour, small nicks and cuts that stopped bleeding almost immediately, bruises they barely felt, but it was clear that both had intended to inflict much worse.

The audience went wild, howling and cheering for their champion of choice!

Weiss blankly stared at the arena. “What the hell?!” she screamed over the din. “What just happened?!”

“Didn’t catch any of that, Weiss?” Yang yelled back.

“No!” Weiss cried. “That was over in seconds, how did any of you see that?!”

“Training and/or Fae genetics, maybe a little bit of mutations as a consequence of living here!” Taiyang replied. “You can probably just watch the recordings later in slow motion!”

“That’s not what I’m--”

Weiss’ words were cut short as Qrow and Winter charged at each other again.

Knees were driven into stomachs, and heads if they could reach. The hilts of their swords were bashed and smashed against each other. Winter got a good grip on Qrow, wrestled him into the ground. Clouds of dust kicked up all around them as she tried to stab him.

Qrow twisted, kicked, grabbed and wrenched Winter’s sword away, anything to keep her from hitting something vital, until he managed to scramble out from under her.

“What happened to you winning this fight in the blink of an eye, Qrow?!” Winter snapped as she watched him flee. “Found you underestimated me?!”

“Nah, just holding back a little to be be polite to you!” Qrow said as he got back up on his hind talons, put pressure on the worst of his injuries. “It’s considered bad form to destroy your opponents too quickly in a duel—few folks like a curb-stomp.”

“So I suppose you’re going to take this seriously now...?” Winter spat.

Qrow chuckled. “Oh, you bet.”

He charged again, Winter braced herself.

Clang!

Their swords locked, Winter forced her blade down on Qrow’s shoulder. It grazed the armour there as he spun away from her, his arms twisting unnaturally—for a human, at least. He slashed at her side, Winter cried out and jumped back.

Her eyes were wide as she looked her new wound, then at Qrow casually bending his limbs back to their normal appearance.

“Yeah, naturally double-jointed—opens up some real interesting avenues of attack, among other things,” he said. “If you surrender now, I could show you them, how to counter it with the range of motion you’re limited to.”

“No thank you,” Winter replied, “I prefer to learn by doing.”

Qrow shrugged. “If you say so, Ice Queen,” he said, before he attacked again.

Strikes came at unexpected angles, Winter could only deflect or dodge them, little time nor opportunities to make a counterattack. Qrow began to make full use of his talons, deflecting Winter’s blade, tearing into her armour like daggers, digging into her and showing just how hard it really was to escape his grip.

Winter lunged, Qrow latched onto her shoulders and vaulted right over her. Winter spun around, she cried out as Qrow’s hind talons slashed at her face, a second kick wrenched her sword right out of her hands, and sent it flying away!

Winter gritted her teeth as she raised her hands in front of her, one eye closed for the blood dripping down.

Qrow lowered his sword, gestured to Winter’s fallen weapon. “Pick it up,” he said, “I want to win, but not like this.”

Winter growled at him as she carefully inched to her weapon, an eye always on Qrow.

“Oh come on!” Weiss yelled over the howling of the crowds. “How was that even legal?! That can’t possibly have been legal!”

“It’s legal!” Ruby said. “’Only whatever the combatants naturally have’ means everything—talons, super senses, tails, you name it! Otherwise, we’d have all kinds of complicated rules and exceptions depending on the species of whoever is fighting!”

“I wouldn’t worry too much about it, though!” Yang said. “She’s doing pretty good for someone who’s never had to fight a bird Fae head-on before!”

“What makes you say that?”

“She’s not dead or lost a limb yet!”

Weiss punched her.

“OW!” Yang cried, clutching her arm, frost seeping into the skin. “I was legitimately complimenting your sister!”

“Well it was a shitty compliment!”

As Ruby and Taiyang worked to prevent another fight breaking out, Winter picked her sword back up, wiped the blood off her face now that the bleeding had stopped.

“Sure you still want more, Ice Queen?” Qrow called out. “You didn’t seem to be doing too good just now!”

“Only because of surprise, Qrow, only because of surprise!” Winter fired back. “I’m rather sure I know how to fight you now—as a matter of fact, I invite you to strike me again, so I can demonstrate.”

“Painfully obvious trap aside, you really sure about that?” Qrow asked. “I respect enough to tell you I’ve still got more up my sleeves—and those are just the tricks I can legally use here.”

“Whatever they are, I am sure I can handle them, so bring them out, Qrow!” Winter said as she got into a combat stance.

Qrow smirked. “Don’t say I didn’t warn you.”

He threw his sword into the ground, blade first. Winter watched in confusion, until Qrow removed the armour from his arms, detached his robes’ sleeves and revealed the thick black feathers underneath. He spread his wings out wide, before he blasted towards Winter, his sword clutched in his rear talons, clouds of dust getting kicked up in his wake.

Weiss whipped her head around to Taiyang, Ruby, and Yang.

“Still legal!” they all said.

Winter braced herself as Qrow neared, her teeth gritted. She shut her mouth as he shot upwards and over her at the last second, blasting a cloud of dust at her. Qrow swooped down around her, kicking up more and more dirt, completely obscuring them from both from view.

“WINTER!” Weiss screamed.

Outside the cloud, Zwei and the crowds nearest to it covered their eyes as the shield generators kicked in, creating gusts and blowing it back into the arena.

Inside, Winter stood braced, tensed, and blinded. The excited chatter of the crowds was deafening, but not so loud that it completely drowned out the sound of flapping wings, the hum of the shield generators, and most importantly, a sudden rush of air.

Whoosh!

Qrow dove at Winter, slashed at her side.

Winter gritted her teeth, swung her sword through empty air.

Whoosh!

Qrow struck again, flying low and catching her on her leg.

Winter jerked it back, held her sword tightly as she listened to the air blowing all around her.

Whoosh! Clang!

Winter’s blade met Qrow’s, deflected it upwards and away from her.

Qrow frantically flapped up and away, Winter spun around, dropped her sword as she dashed forward and took a leap into the air!

Qrow broke out of the cloud just in time for the crowds to see Winter tackling him from behind, pummel and strangle him until we was forced to land, face-first in the dirt. He struggled and flailed, but Winter was merciless, raining down punches on him, ending with one solid hit to the back of his head!

Crack!

Qrow’s head dropped into the dirt, Winter got off of him and stood back up. He rolled around, coughing and wheezing, he choked as Winter brought her foot down on his neck. He tried to grab at his leg, she stepped down harder.

He choked again, he put his arms down flat.

Weiss let go of the breath she had been holding. “Oh… good… does this mean she’s won?”

“Nope!” Taiyang said, shaking his head.

“What do you mean ‘Nope!’?”

“All duels have to have a mutually agreed upon, unmistakable gesture that you’ve surrendered, in case you can’t say it out loud—a non-verbal safeword.” Taiyang explained. “I don’t see either of them making anything close to that.”

Back down in the arena, Winter took her foot off Qrow’s neck. “Pick it up,” she said, pointing to where Qrow had dropped his weapon. “I want to win, but not like this.”

Qrow sucked in a breath as he bent his arms back to normal, his feathers folding close to his skin. The both of them kept an eye on each other as he retrieved his sword, before they calmly moved back to the center of the arena, stared each other down as they readied their weapons.

An ominous quiet fell over the crowd; even Nora, who had been enthusiastically cheering the whole duel, went silent.

“What’s going on...?” Weiss asked.

Almost everyone around her shushed her, Weiss flinched.

Ruby offered her hand, Weiss gripped it tightly, sweat pouring down her head, her gloved hand pulsing and pouring magic into Ruby.

Winter and Qrow charged at each other one more time, not two clean, decisive, faster-than-the-eye-could-see slashes as in the holos, but a messy, desperate explosion of violence, filled with punches, slashes, grapples, kicks, thrusts, and finally, a headbutt that sent the one who gave it, Winter, and the one who received it, Qrow, staggering backwards.

The crowd collectively held their breaths as both combatants struggled to remain standing. Winter suddenly dropped her sword, clutched at the worst of her wounds. Qrow chuckled softly until he noticed the bloody feathers falling from his head, the blood dripping into his eyes.

Then, the two of them fell to their knees or dropped flat on the ground.

<MENDERS!> Ren yelled.

Zwei, Blake, and the emergency menders sprung into action, trying to stem the worst of the bleeding, or just hauling them to the medical tent as quickly as possible.

Nora blew the horn, yelled, <And so this duel ends with a draw!> before she, Ren, and Penny climbed down from the commentary box to help.

The crowd went berserk, cheering and howling from the aftermath.

Weiss groaned, before she fell limp against Ruby.

“There, there, Weiss...” Ruby said, patting her with the hand that wasn’t half-frozen.

Note: Fae saying, roughly translated: “If you don’t want to fight in the trees, don’t fight a monkey. If you don’t want to fight in the air, don’t fight a bird. If you don’t want to fight in the water, don’t fight a fish.”