#microlit

[touchflesh]

new post !!!

liberated sisyphus searches for objects of a similar weight and finds the most frail grandmother moving to the top floor apartment with a Refrigerator

[microlitseo]

How to Handle Volatile and Hazardous Liquids with a Pipette?

Working with volatile and hazardous liquids in a laboratory needs to be done with precision, safety, and proper equipment to prevent contamination, spillage, and exposure to toxic chemicals.

The liquids in an experiment need to be handled when transferred from one device to another. It is risky as well as affects the overall experimenting time and processes.

There are various places such as in a medical factory plant, or an industrial facility, where the person will be asked to learn the process of fluid transferring.

In this blog we discussed guidelines on best practice liquid handling using pipettes and other liquid handling devices for ensuring efficiency as well as safety.

Having Knowledge of the Dangers of Hazardous Liquids

Flammable and dangerous liquids comprise acids, bases, solvents, and biological samples that may harm individuals if not handled properly. Risks posed by these chemicals are:

Inhalation Risks: Certain volatile liquids evaporate rapidly and release toxic vapours.

Skin Contact: Toxic and corrosive chemicals can result in burns, irritation, or allergic reactions.

Cross-Contamination: Handling may contaminate samples, causing inaccurate test results.

Fire and Explosion Hazards: Flammable liquids are prone to ignition if improperly handled.

Selection of Suitable Liquid Handling Equipment

The selection of suitable equipment for handling dangerous liquids reduces risk and enhances accuracy. The most important equipment are:

Micropipette

Micropipettes are typically applied to accurate liquid transfer in tiny amounts. Filter tips should always be employed during handling dangerous liquids with the micropipette to avoid aerosols reaching the pipette's internal mechanism.

Best Practices:

Use disposable tips to avoid cross-contamination.

Calibrate often for precision.

Avoid over-pipetting to reduce exposure.

Bottle Top Dispenser

A bottle-top dispenser is best suited to dispense hazardous liquids directly from reagent bottles. This minimises exposure to fumes and spillage.

Best Practices:

Select dispensers with a recirculation valve to avoid dripping.

Use models for corrosive liquids.

Use proper PPE when dispensing.

Acid Dispenser

An acid dispenser is designed particularly for the safe handling of corrosive acids and bases. Dispensers may come equipped with chemical-resistant materials that guarantee durability and reliability.

Best Practices:

Always test compatibility with the acid being handled.

Work in a well-ventilated space to minimise fume accumulation.

Cleaned thoroughly after every use to prevent corrosion.

Electronic Pipette Filling Instrument

An electronic pipette filler instrument fills liquids electronically, eliminating liquid aspiration and dispensing, which reduces manual manipulation of volatile liquids.

Best Practices:

Set the speed controls to the viscosity of the liquid.

Charging these liquid handling devices with a full charge before use is important.

Use in combination with safety cabinets where highly toxic chemicals are handled.

Safety Precautions in Handling Hazardous Liquids

Irrespective of the instrument used, some safety procedures are essential to be followed all the time:

1. Proper Personal Protective Equipment (PPE)

Wear lab coats, gloves, and safety goggles constantly when working with hazardous liquids. For volatile chemicals, wear a respirator or use a fume hood to reduce inhalation hazards.

2. Work in a Well-Ventilated Area

Including volatile liquids in the laboratory can emit fumes that are toxic to inhale. Work at all times in a fume hood or well-ventilated area to let these vapours evaporate. This could also prevent you from suffocation which may result in several respiratory problems.

3. Do Not Pipette by Mouth

Mouth pipetting is entirely forbidden since it can cause ingestion of harmful chemicals. A mechanical or electronic pipette pump can always be used. They can also play a significant role in streamlining experiments and reducing the chances of liquid loss.

4. Dispose Waste Correctly

Carcinogenic liquid waste has to be disposed of following rules. Always use proper containers and do not pour chemicals into drains except for specific instructions while working in the laboratory. This will prevent your direct contact with the liquid waste and also reduce the risk of getting affected.

5. Label Correctly and Store Liquids Properly

Dispose of liquid hazardous wastes in mislabelled, chemically resistant containers. Separate incompatible products from one another to prevent dangerous interactions. Experimenting in the laboratory will keep the process smooth when you have the labelled containers in front of you. 

Dealing with Emergencies

In the laboratory, when you are being careful, then also at time accidents may occur. Various steps can be used to prevent accidents. Here are the ways how to deal with an emergency:

Spills

There can be a moment when a solution gets spilt in the laboratory. You can work on containing spills instantly with absorbent material. Immediately, neutralise acids and bases before cleanup, or else they can affect the laboratory atmosphere and also affect your experiment processes.

Exposure

Gloves and other PPE kit instruments are required while working in a laboratory. But there can be an instance when the solutions can contact with your skin. You should wash instantly for a minimum of 15 minutes. Call the medical help if necessary.  

Inhalation

If you have inhaled the harmful and hazardous solutions in the laboratory. Evacuate to fresh air at once and get medical attention. This can prevent your health getting deteriorating, which would also keep you healthy throughout the experiment processes. 

Fire

Apply a suitable fire extinguishing agent (e.g., CO2 for flammable organic solvents, dry powder for burnable metals).

In case of fire, while experimenting, this can be a hazardous issue. First, apply a suitable fire extinguishing agent (e.g., CO2 for flammable organic solvents, dry powder for burnable metals). This would play a significant role in reducing the burning sensation and also reduce the further fire effects. 

Conclusion

Proper equipment, safety procedures, and expertise in correct techniques are needed to handle volatile and dangerous liquids using the pipette. Liquid handling devices such as a bottle top dispenser, micropipette, acid dispenser, and electronic pipette filling devices provide accuracy and reduce hazards. Safety procedures and best practices allow you to have a safe working environment while producing accurate results in your liquid handling process. If you are looking for safe and suitable experiment instruments, without further ado, you can connect with Microlit to get your preferred instruments.

[chekhovsclaymore]

So, I've been thinking about it, and you only really need to know 2 things about Micah in order to understand him as a person.

The 1st is that if you asked him what he cares about most in this world, he would tell you that the ONLY thing he cared about was his own survival, and that he would throw anyone and everyone he's ever loved under the bus if it ensured his survival. He'd tell you that he'd lie, and steal, and manipulate, and kill if it kept him alive, and it doesn't matter how many times his actions directly contradict that belief, because you will NEVER convince him otherwise. He will risk his life just to make someone else happy, then look them dead in the eye and call himself the most selfish person who's ever lived, and he truly believes that about himself with every fiber of his being. He thinks he's a horrible person for SURVIVING.

The 2nd thing you need to know to understand him is that he cares WAY too much about what other people think of him, and it's not because he's insecure. He views himself as very competent and intelligent, and he takes pride in that, but he has an undying NEED for other people to KNOW that he's intelligent and competent. He desperately craves approval, and he's been STARVED of it for a long time, so Micah will do incredibly stupid things to prove to people how smart he is.

So, Micah views himself as someone who is extremely competent at being horrible, and it is BOTH a source of pride and shame for him.

[caesarsaladinn]

🌻

did you know that molecular biology chemicals can go extinct?

the most versatile way to visualize proteins in an organism is by creating antibodies (i.e., immune system proteins) that will bind to that protein and nothing else. some of these antibodies are produced only by one cell strain, maintained and distributed by a single lab somewhere, and if that strain gets contaminated, or dies out, or just stops making the antibody, it’s gone for good.

this is most often the case with older antibodies, because they weren't produced with any DNA or protein sequence in mind--the cells in question were just given an antigen (i.e., the protein you want the glowing antibodies to bind to) to fight, with basically no idea which *part* of the protein that antibody might be recognizing. ergo, you could only replicate it by starting from scratch.

it would be a good scam, though. you could make a killing by selling counterfeit vintage antibodies and then telling the researchers who write angry emails about how it didn’t work that their lab must not be clean enough, or they left it out of the fridge for too long, or whatever.

I'm an undergrad with barely pocket money to my name and even I would spend a hundred bucks on a quarter of a milliliter of FGF/Wnt binding fluorescent protein. but afaik it no longer exists!

[liquidhandlingproduct]

Microlit - Elevating Precision Liquid Handling for Your Brand

At Microlit, we offer a comprehensive suite of liquid handling instruments, including Bottle Top Dispensers, Micropipettes, Electronic Burettes, Pipette Controllers, and Pipette Tips. Our reliable solutions are designed to enhance both the efficiency of your operations and the reach of your brand.

[girinma]

anyway frothing at the mouth looking at my scheduled boat shifts because I need that overtime pay

[thydungeongal]

As someone who genuinely likes d&d 5e, what OSR games would you recommend?

Five Torches Deep is a neat little "OSR by the way of 5e" type of game that takes the basic mechanics of D&D 5e, simplifies them a lot, and then injects a lot of missing OSRisms like wandering monsters and somewhat deeper procedures for dungeon-crawling in. It's good if you want to try out something OSRish but want to keep the core mechanic of rolling d20 plus modifiers for most things instead of the singleton mechanics of older editions of D&D. It is decidedly stripped down at its core but it has a lot of support in terms of optional rules and additional content that can be added on top of it.

Shadowdark is one that I keep hearing about a lot: it's a decidedly dark fantasy take on D&D 5e but, again, simplified and with lots of OSR style mechanics ported back in that are missing from core 5e. One neat mechanic is that torches provide light for one hour of real time, meaning that, like their characters, players are incentivized to make decisions decisively and quickly. I have not played nor read Shadowdark but it also has a lot of community support and the few things I have seen of it look promising.

Finally there's FORGE which is a free OSR style RPG with some modern quality of life added in and it's honestly a very nice, full game with everything you need to experience OSR style play but presented in a way that is easy for newcomers to understand.

Other things worth mentioning: the Microlite family of RPGs, Basic Fantasy Role-Playing Game, Mausritter. If you have the time and will, these are all worth looking at if just to know what's out there and to be better able to make an informed decision.

[wolfjackle-creates]

Bring Me Home, Chapter 2 Part 3

A little shorter this week. I had my graduation ceremony over the weekend and the opportunity to hang out with my sister-in-law for the first time in a few months! (She and my brother moved states a few months back.) If you scroll down a bit, you'll be able to see how I decorated my graduation cap! I love how it turned out.

But you don't care about that. It's Wednesday! Time for a WIP Wednesday segment!

Story Summary: Tim and Danny are both neglected by parents who care more about their work than their families. They deal with this by spending too much time online and find each other playing MMORPGs. They keep up their friendship as Tim becomes Robin and Danny becomes Phantom and don't bother keeping secrets from each other.

First, Previous

Word Count: 1k

----------

Unable to get Tim’s attention, Conner asked, “Who’s Technus?”

Danny shrugged. “One of my rogues. Tuck thinks he’s the ghost of Nikolai Tesla. He’s interested in controlling all technology and will make himself a giant mechasuit cannibalized from any electronic he can find in, like, a half mile radius. Super annoying.”

Tim hummed. “You didn’t tell me about him being Nikolai Tesla.”

“It’s a new hypothesis of Tuck’s. He’s been trying to research all the ghosts that come through as part of our profiles on them. That involves trying to figure out who they might’ve been in life. We’re hoping it’ll help me deescalate confrontations to cut back on property damage. Thanks to my parents talking about how evil all ghosts are, no one trusts Phantom and I get blamed for everything.”

Tim reached out and squeezed Danny’s shoulder. Practically everything Danny ever said about his parents made him like them less. To change the subject before he learned something else that’d make him want to attack Jack and Maddie while they were under the same roof, he asked, “So why does ectoplasm harm electronics anyway?”

Danny seemed to lean into his touch. “Well, ectoplasm is complicated. It is generated in this dimension but doesn’t really belong here. It comes about through death and leads the way to the Ghost Zone. At least… that’s the hypothesis I think is the most likely. I’ve only really been studying it for a few months since my own accident, though.” He shook his head. “Anyway! When it interacts with things on Earth that aren’t trying to get to the Zone, things get weird. Especially with non-sentient things that can’t will the ectoplasm to act in a specific way. Even animals can exert some control over ectoplasm. But electronics can’t.”

It was only a few minutes more before Danny had completely disassembled the phone. He then grabbed another pipette and adjusted the volume and added ectoplasm to certain pieces. Then took a third size and did it all over again.

“How on earth did you find out how much to add?” asked Bart. “You’re changing quantities constantly.”

“Trial and error. Long and tedious trial and error. We tried dipping sections in the ectoplasm to start, but that generally fried the tech and mutated its function. Wires do do best with submersion, though. No more than a second or two for small ones. Even after we stopped submersion, we started by adding way too much—spreading it over the entire chip. But that also didn’t work. Realized just half a microliter applied to the connections was best. The camera, speaker, and microphone need more. Those get ten microliters apiece. And we just kept trying different amounts until we had something that worked. We ruined four phones before we started testing each component individually.”

Conner let out a low whistle. “Well we’re glad you have. Thanks for helping with this.”

“Of course. Anything for Tim.”

Tim’s face heated as Cassie laughed. “Yeah, our Tim has a way of winning people over, doesn’t he?”

“I think I won him over, actually.” Danny hung the pipette back up on the holder. “All right, now just to put this baby back together. Who’s hungry?”

“Me!” called Bart. “It’s been ages since we’ve last eaten.”

“You’ve got an accelerated metabolism, right? We’ll stop by a store and get some extra stuff if you need anything overnight or tomorrow.”

“I like you,” said Bart. “You should come with us when we leave. Join our team.”

Tim buried his face in his hands, did none of his teammates know the definition of subtlety? Offering Danny a place with the Teen Titans or Young Justice was the first thing he tried.

“Thanks for the offer, but as I’ve told Tim, I can’t leave Amity. No one else is capable of responding to ghost threats.”

Conner shook his head. “Looks like your parents have it under control.”

Danny laughed. “Oh hell no. They’ve got a lot of inventions and most of them do something. But it’s not always what they expect them to do. And dad’s aim is terrible.” As he spoke, he continued to reassemble Tim’s phone.

Tim couldn’t help but admire how expertly Danny’s fingers moved over the pieces. And before he knew it, Danny was handing the phone back to him.

“Should work now. Turn it on and double check.”

Tim took it and held the power button until the WE logo appeared. Sure enough, once the screen loaded, so did a dozen missed phone calls and even more missed texts.

Bruce, Dick, and Barbara had all attempted contact multiple times. Even Alfred had called once. He winced and immediately called Bruce back.

“Hey, B,” he said as soon as the call connected. “We’re all fine. Just crossed an area that messed with our tech.”

“How did it mess with your tech?” Bruce demanded.

“It’s normal in this area. But I’ve a local friend and he fixed my phone. He’ll take care of Conner’s, Cassie’s, and Bart’s after we grab some dinner. So if anyone else is worried, tell them we’re fine and they can call me in the meantime if they have questions.” Tim made sure to use civilian names so Bruce would know they were no longer in costume.

“Who is this ‘friend’?” asked Bruce.

“God, B, it’s fine. I’ve known him for years. We game online together when we can. Have since we were kids.”

“Hn.” Why was it so much harder to read Bruce over the phone than in person? It was so annoying. “I see. Where are you currently?”

“We’re in Illinois. Will probably stay here a day or two with Danny and his parents. And then we’ll come home and share everything about our trip.” Aka, submit an official report about the outcome of their mission.

“Very well. I expect to know all the details. And I want twice daily check-ins until you’re home.”

“Fine, fine. Will do. Bye, B.” Before Bruce could demand anything else, Tim hung up on him. Next he shot texts to Dick, Alfred, and Barbara assuring them he was fine and his phone was working again. Replies came instantly and he ignored them all. “All right, that’s done. Let’s go eat.”

---------

Next

I think this is the first time I've had an actual scene break to stop the segment at. I usually just go until I see a change in the conversation, but I've got my <hr> marker at this point and there's gonna be a scene change! (So I won't have to repeat a paragraph or two next time I post.)

You get a different explanation for ectoplasm in this fic! Wasn't planning on that, but it happened and I like it.

Hope you enjoyed.

Tag List Part 1

@gremlin-bot, @bonebrokebuddy, @britcision, @lady-time-lord-, @welcometosasakiworld, @akikkobara, @phoenixdemonqueen, @dolfay, @skulld3mort-1fan, @we-ezer, @markus209, @sjrose1216, @onyxlightdragon, @dragonsrequiem, @jesus-camp-the-sequel, @spidey29phangirl, @kyrianclawraith, @evilminji, @introvert-even-on-the-internet, @emergentpanda-blog, @lexdamo, @v-inari, @idontgetpaidenoughforthisshit, @longlivethefallen, @undead-essence, @xye-chan, @liandrin, @seraphinedemort, @kisatamao, @schalensitzbucket, @caelestisdreamer, @runfromthemedic, @nutcase8691, @channajen, @tonicmii, @ambiguouslyominous, @vythika96, @addie-lover-of-stories, @ironicvixen, @violetfox2, @pickleking8, @mysticalcomputerdetective, @ark12, @mygood-bitch99, @squirrel-wolf, @satisfactionbroughtmeback, @sometimesthingsfallapart, @automaticsoulharmony, @d4ydr34min9, @revnantdpxdclover, @midigeria, @raginblastocyst

[theclockworkkidart]

(JJBA LotL AU) I redrew it because I wanted to. Link to the original that’s (wheezes) two years old now (link).

Acanthite (Sorbet) is shattered by Lunarians while trying to protect Orthoclase (Gelato). A hardness of 2.5 meant it didn’t take much. Parts recovered and reassembled.

Anatase (Illuso) is accidentally covered in Cerussite's (Fugo) toxic metal. Parts need to be carved off and Anatase ends up losing some memory but ultimately recovers.

Wavellite (Pesci) is shattered in a fight against Lunarians, Celsian (Prosciutto) already damaged. The Lunarians were defeated but recovering Wavellite’s body from the sea took a bit of time.

Celsian (Prosciutto) heavily damaged in a fight against the Lunarians. Watched Wavellite nearly being abducted and attempted to fight but ultimately the others got rid of the Lunarians.

Strengite (Melone) shot with a Lunarians arrow through the mouth. Strange attack as Strengite is a long distance weapon fighter and he was positioned in the building looking for the Lunarians.

Arctite (Ghiaccio) shot through the neck with a Lunarian arrow.

Microlite (Formaggio) severely damaged during a fight with Lunarians, especially to the face where both eyes ended up damaged and attempted to battle blind. Recovered.

Orthoclase (Gelato) caught in a Lunarian trap as Acanthite was damaged. Orthoclase being the soldier and Acanthite not took a mental toll.

And finally Eucryptite (Risotto) damaged in a downpour of Lunarian arrows.

All of them were recovered 😌.

[dieletztepanzerhexe]

The most recent culture of the Upper Palaeolithic (the Late Upper Palaeolithic) in western and Central Europe is the Magdalenian, 20,000 - 12,500 years ago.

A man of the Moravian Magdalenian. He holds a spear-thrower using the principle of leverage, and a spear with a bone projectil point with saw-like razor-sharp microlites. The horses on his clothing are depicted according to a decorated object found in the Pekárna Cave (the Moravian Karst).

In front of the tent in the Pekárna in Moravian Karst cave. The large cave didn´t espace notice of the Magdalenian hunters, which were tracking the cariboos herds around the karst areas. In the cave of Pekárna, the rank of fireplaces was discovered across the corridor, which was probably the standing place of the tents. The cave climate was convenient for fabrication of the cariboo meat. The karst area enabled the hunters to use the terrain for building perfect traps.

Fascinating artefacts found in the Pekárna cave: tiny bone needles, Silicit tools, embedded into boned or horn handgrip, harpoons, blade-liked, decorated artefacts and fascinating portrayals of the scenes of the wildlife on the horse ribs (grazing horses, fighting bisons).

A camp of reindeer hunters of Petersfels

The picture takes us to southwest Germany, the well-known site of Petersfels by Engen. The narrow valley became a trap for hunted reindeer. Reindeer were killed there on a large scale, as the remains of their bones witness. The peculiarity of this picture is that the photographic studies were not created artificially, but were made just on the site, where well-versed fans displayed their home-made clothes, tents and Magdalenian weapons

Notice the antler heads of the throwing weapons, which were made so well that they can be compared with the artistic level of carving in butts of the recent firearms. 

One of the most beautiful and imaginative engravings on the thrower represents two fighting Capricorns. The Trois Frères cave in France. The image reconstruction represents the artefact in it´s original form (the heads of the Capricorns are not preserved).

A Magdalenian bone points with microlites A bone point from the Drátenická Cave in the Moravian karst. This artefact is now in the Institute Anthropos, which is a part of the Moravian Museum (Moravské zemské muzeum) in Brno. You can see (the lower part to the right) an impression of the former careful and fine binding. The reconstruction depicts the procedure of its piecing together.

The convenient terrain helps during the hunting. The Northern societies often hadn´t have enough members for driving the gregarious animals. Therefore, they built stone pillars, which the hunted cariboos were not able to distinguish from the hunter.

The bone needles found in abundance both in Petersfels and the Moravian Karst are different from those of the Gravettian. The woman in the picture is sewing a piece of clothing using an awl and a needle. The man is repairing the bone point of a weapon. He easily replaces individual broken segments of the cutting edge with new ones. To the left from him, there is his equipment, such as throwing weapons, lamps, a chieftain’s baton, a flute and bone points.

The reconstructional imitations of clothes dating back to the era of the Magdalenian reindeer and horse hunters. The first clothing is exhibited in the Museum of Engen. You can see decorative patterns made according to an ornamented shovel-like object found in the Moravian Karst on the right clothing.

A spear-thrower  was ideal for hunting animals living in herds, such as horses and reindeer. It was capable of shooting a maximum amount of projectiles within a minimal period of time. The accuracy of shots at a mass of running bodies was unimportant, the work was completed by sharp points. Most likely massive stone pillars were used when driving reindeer. Reindeer avoided them in the same way as they avoided people. That is why the Magdalenians preferred such environment where limestone screes, of which they erected the pillars, were available, e.g. outcrops of limestone, which are characteristic of the karst areas. The whole life of these people was closely connected with animals living in herds, and it formed their culture. The Magdalenian settlements came to Central Europe, namely to Poland, 16,000 years ago and to Moravia about 13,000 years ago

The representation of women, the unmistakable Magdalenian Venuses, was quite curious. Women’s figurines hardly bore any detail, they were often just outlined, and their bottoms were always stuck out. Some small figurines had holes for hanging.

One of the few Magdalenian Venuses that provided at least some information about her hairdo.

[touchflesh]

turkey gizzard and the shitty wizard

up now on patreon

TOUCHFLESH.COM

TOUCHFLESH.COM

TOUCHFLESH.COM

[microlitseo]

Pipetting for Molecular Biology: Tips and Tricks

Pipetting can be considered one of the important processes of molecular biology, it acts as a foundation on which various experiments are conducted. This does not depend on the preferences of usage, even if they are used by a seasoned researcher or a budding student researcher. 

This follows with the process of mastering pipetting techniques is crucial for accurate and reproducible results. The overall pipetting process with micropipette is more than just pressing a button, as it is delicate, consistent, and craves precision which produces finest results. 

This blog is a guide to enhance your pipetting techniques and processes which can be seamless while you are in a laboratory, along with understanding your pipette. It also includes various essential tips and tricks which can transform your experimenting and  make you a pipetting maestro. 

Understanding your Pipette

Before carrying out an experiment in molecular biology, you may need to understand the pipette and factors you can consider while in a laboratory. There are various ways to understand the pipette such as,

Choose the Right Pipette

Like any other laboratory equipment, pipettes also come in various sizes, including volumes from microliters to milliliters. This can provide you with a wide range in sizes from which you can use for your required pipetting. 

Incorporating pipettes as a acid dispenser, using for required volumes can play a significant role in experimenting. Further, if you miss to get the accurate size of the pipette, you may affect the accuracy of the results at the end of the experiment. 

Calibration is Key

When you connect your pipettes with other liquid handling instruments, you may need to precisely calibrate before conducting the experiment. This can play a major role in providing you with a precise volume of liquids transferred during experiment. 

Along with this, you must be conducting experiments in a laboratory, so you can follow your lab’s calibration schedule. This can wither off the hesitation of calibration if you ever suspect any inconsistencies. 

Understanding Pipetting Mechanics

Along with working in a laboratory, you will be getting familiarized with the pipetting mechanics. It would also make you aware about the two stop positions while working with the pipettes. Including the electronic pipette pump, the first stop will be for aspirating the liquid and the second stop for dispensing the remaining liquid.

Tips and Tricks for Precision

In a laboratory, during the experiment in molecular biology you would need an enhanced precision which will also eventually, provide you with accurate results. There are multiple tricks for required precision which you can follow like, 

Vertical Pipetting

There can be various ways for pipetting during an experiment, when the experiment procedures are connected with the bottle top dispenser.  You can follow processes of vertical pipetting which can ensure the accurate aspiration. 

Vertical pipetting processes during an experiment in a laboratory will be significantly helpful, if you are dealing with small volumes of solutions throughout the experiment. 

Pre-Rinsing the Tip 

During an experiment in a laboratory, you can use the processes of pre-rinsing with the solution of the tip of the equipment. This saturates the air space in the tip and minimizes the volume discrepancies. As it prevents bubble forming, which can provide you with the accurate results through the experiment.

Angle for Dispensing

While you are in a laboratory, dispensing liquids from one equipment to another can make way through the experiment. So when dispensing at first touch the tip against the wall of receiving the tube at a slight angle. 

Along with the experiment processes, it can play a significant role in ensuring a complete dispensing of the liquid. Eventually, this will support your overall process of transferring liquids and provide you with accurate results. 

Viscous Liquids

Viscous liquids are the liquids which flow resist, which can slow down the pipetting speed and consider using reverse pipetting techniques. This can provide support in your experiment processes, which can also lead to seamless and accurate results. 

Using the right tips

This can be one of the important points among the tips before you carry out suitable pipetting techniques. It will also follow by choosing filtered tips for sensitive applications to prevent aerosol contamination, which will play a significant role in avoiding mixing of foreign elements in your experiment solutions. 

Avoiding Common Pitfalls

Air Bubbles

Bubbles can affect the pipetting standards, evidently by affecting the experiment results. You may need to avoid the bubble formation by immersing the tip properly. Further, this will allow you to pipette smoothly. One of the other ways to accurately get the results is to pre-rinse the tip first before starting with the experiment. 

Contamination

In an experiment, you must be dealing with multiple solutions and laboratory equipment, which makes it important to keep all the parts contamination-free. You can change tips between each solution which will prevent cross-contamination.

Along with this, you can also follow aseptic techniques such as hand washing and sterilization with sterile solutions. These will prevent contamination and also keep you safe from the infection if you catch them through the experiment. 

Conclusion

As you get used to conducting pipetting for molecular biology, you would gain an indispensable skill while experimenting. This will be crucial for achieving accurate and reproducible experimental results. 

By understanding your pipette, from selecting the right size to ensuring proper calibration and by following precise techniques in an experiment. Through which you can significantly enhance your laboratory work. 

Furthermore, you can recognize and avoid common pitfalls like air bubbles, along with the contamination which will maintain the integrity of your experiments. This will also let you get the accurate pipetting results. 

Eventually, consistently following the pipetting standards in molecular biology, this will transform your pipetting from a task to refined consistent art. It would also contribute to your results in the lab and build a foundation of your molecular biology experiments. 

Are you also a laboratory technician and looking for suitable pipettes or equipment for your experiments. Without further ado, you can consult Microlit today for making a leap in your experiment standards and elevate your pipetting standards.

[delightfully-deranged-dagur]

Found this really good TOA fic and I had to draw the main OC for it.

https://archiveofourown.org/works/53121163/chapters/134408227

The fic has some smut in later chapters so be warned

Micah Martinez, aka Microlite. Had to draw Douxie & Zoe too of course.

[mysticalblizzardcolor]

Amazing Kunzite with Tourmaline, Microlite and Cleavelandite From Darai Paech, Kunar Province, Afghanistan.

[liquidhandlingproduct]

Adjustable vs. Fixed Volume Micropipettes - Which is Right for Your Lab?

In the precision-centric world of laboratory science, the choice of pipetting tools is crucial to the efficiency and accuracy of experiments. Among the essential tools in any lab are micropipettes, specifically adjustable and fixed volume micropipettes. Each type has its unique advantages, and choosing between them depends on your lab's specific needs. Let's delve into the characteristics of variable volume micropipettes, Microlit micropipettes (a renowned brand known for its precision tools), fixed volume micropipettes, and adjustable micropipettes to help you make an informed decision.

Variable Volume Micropipettes

Variable volume micropipettes are versatile tools that allow researchers to adjust the volume of liquid dispensed, making them suitable for a wide range of applications. This adjustability is particularly beneficial in experimental setups that require different volumes or in labs with varied pipetting needs. Variable volume micropipettes ensure that you can perform multiple tasks with the same pipette, reducing the need for multiple fixed volume pipettes and saving space and resources.

Microlit Micropipette

Microlit is a brand known for its high-quality, ergonomic pipettes designed to provide accurate and precise liquid measurement with reduced user fatigue. They offer both adjustable and fixed volume micropipettes, catering to the diverse needs of laboratories. Microlit micropipettes are engineered with user comfort in mind, featuring lightweight designs and easy volume adjustments. Their products are an excellent choice for labs looking for reliable, ergonomic pipetting solutions.

Fixed Volume Micropipettes

Fixed volume micropipettes are designed to dispense a specific volume of liquid. These pipettes offer simplicity and speed, as they eliminate the need to adjust the volume for each use, making them ideal for repetitive tasks requiring the same volume. Fixed volume micropipettes are known for their reliability and consistency, providing precise measurements every time. They are particularly useful in high-throughput settings where the same procedure is repeated multiple times.

Adjustable Micropipettes

Adjustable micropipettes combine the versatility of variable volume pipettes with the precision and ease of use characteristic of fixed volume models. They allow for quick adjustments of the dispensed volume, catering to a broad range of tasks within a single experiment. Adjustable micropipettes are perfect for labs that handle complex protocols requiring different volumes but still demand the precision and accuracy that fixed volume pipettes offer.

Which is Right for Your Lab?

Choosing between adjustable and fixed volume micropipettes depends on your lab's specific needs:

For Versatility and Broad Application Range: If your lab performs a wide variety of experiments that require different volumes, variable volume or adjustable micropipettes are the way to go. They offer the flexibility needed to accommodate diverse protocols.

For Repetitive Tasks and High Throughput: If you're working in an environment where speed and consistency are paramount, and the tasks involve repetitive pipetting of the same volume, fixed volume micropipettes offer the efficiency and reliability you need.

For Precision and Comfort: Brands like Microlit offer both adjustable and fixed volume micropipettes designed with ergonomic features to reduce fatigue and improve accuracy, making them a good choice regardless of the type you choose.

In conclusion, the decision between adjustable and fixed volume micropipettes should be guided by the specific requirements of your laboratory. Consider the nature of your experiments, the importance of versatility versus speed, and the ergonomic needs of your team. By carefully assessing these factors, you can select the pipetting tools that will best support your research and ensure the success of your lab's operations.