#hiroyuki takano

[cinemaobscura]

Pastoral: To Die in the Country | 田園に死す (1974) dir. Terayama Shuji

[haveyouseenthistoku]

[ruruuds]

[agendaculturaldelima]

#ProyeccionDeVida

🎬 “EL CIRCULO” [The Ring / Ringu]

🔎 Género: Terror / Intriga / Tecnológico / Sobrenatural / Fantasmas / Película de Culto / J-Horror

⌛️ Duración: 91 minutos

✍️ Guión: Hiroshi Takahashi

📓 Novela: Kôji Suzuki

♫ Música: Kenji Kawai

📷 Fotografía: Junichirô Hayashi

🗯 Argumento: En una pequeña y apacible localidad japonesa, entre los estudiantes circula una leyenda en torno a unos videos malditos cuya visión provoca la muerte. Tras la muerte de su sobrina Tomoko, una periodista, Reiko, investigará el origen de dichos videos.

 👥 Reparto: Nanako Matsushima (Reiko Asakawa), Hiroyuki Sanada (Ryuji Takayama), Rie Inō (Sadako Yamamura), Miki Nakatani (Mai Takano), Yūko Takeuchi (Tomoko Oishi), Rikiya Ōtaka (Yoichi Asakawa), Hitomi Satō (Masami), Takashi Takayama (Takehiko Nomi) y Yutaka Matsushige (Yoshino).

📢 Dirección: Hideo Nakata

© Productoras: Kadokawa Shoten & Omega Project

🌎 País: Japón

📅 Año: 1998

📽 Proyección:

📆 Martes 22 de Octubre

🕗 8:00pm.

🎦 Cine Caleta (calle Aurelio de Souza 225 - Barranco)

🚶‍♀️🚶‍♂️ Ingreso libre

🙂 A tener en cuenta: Prohibido el ingreso de bebidas y comidas. 🌳💚🌻🌛

[oliviapurwanti]

Ohsama Sentai King-Ohger Episode 37 English Sub - Full Episodes

Ohsama Sentai King-Ohger Episode 37 English Subtitles

There is an ancient prophecy shared amongst the kingdoms of Earth - 2,000 years after their fall, the Earth Empire Bugnarak will once again rise up to kill all humans. However, five kings and their guardian deity, King-Ohger, will stand up to face them. This is the story of kings who will defend the Earth, as well as a Coming of Age Story for one young man who will achieve kingship henceforth. (Source: powerrangers.wikia.com) Edit Translation

⬇️⬇️Watch Ohsama Sentai King-Ohger Episode 37 Eng Sub - Full Episodes⬇️⬇️

Website : https://dramasian.xyz/search/Ohsama+Sentai+King-Ohger

Related Content Ohsama Sentai King-Ohger: The Secrets of King Racules (Japanese side story) Ohsama Sentai King-Ohger: Adventure Heaven (Japanese side story) Ohsama Sentai King-Ohger: Transformation Lesson (Japanese side story)

Country: Japan

Episodes: 50

Aired: Mar 5, 2023 - ?

Aired On: Sunday

Original Network: TV Asahi

Duration: 23 min.

Content Rating: Not Yet Rated

Native Title: 王様戦隊キングオージャー

Also Known As: Osama Sentai Kingu Oja , Osama Sentai King Oger

Director: Yamaguchi Kyohei, Kamihoriuchi Kazuya, Kato Hiroyuki

Screenwriter: Takano Minato

Genres: Action, Tokusatsu, Sci-Fi

[soon-fm]

Song of the day: “Chubby” by Pitcher56!

I wanted to post “Woman” by them but it wasn’t available on instagram :( so for consistency I’m also posting Chubby here.

Pitcher56 is a Japanese Whisper and Shibuya-kei band from the early 2000’s. Over their short career, they were signed to multiple different record labels. Their discography contains a variety of covers and original works. The three members of the group: Hiroyuki Morita (guitar, bass, drums, and programming), Manabu Takano (guitar and vocals), and Yuki Nakano (vocals) are all doctors today. They released their most popular album “Her Abiding Memory” while in medical school.

[ultra-facts]

Today’s Ultra Trivia:

The man in Ultraman Taiga episode 6 “The Flying Saucer Did Not Come” credited as “The Man Who Wishes To Return to The Stars” is portrayed by Hiroyuki Takano. But back when he was a child actor, Takano had also appeared in the 45th episode of Ultraseven (1967) “The Flying Saucer Has Come”, playing the role of a young boy (the human disguise of Alien Perolynga) luring Saburo into his trap.

This episode of Taiga also pays tribute to episode 45 of Ultraseven, with Takano reuniting with his alien alter ego for the first time in more than 50 years.

[iilluussoorr]

Pastoral: To Die in the Country (1974, dir. Shūji Terayama)

[ghostcultmagazine]

Church of Misery – Atomic Bitchwax - Toke: Live at The Middle East

Church of Misery – Atomic Bitchwax – Toke: Live at The Middle East

Walking into The Middle East in Cambridge, Massachusetts, I realized I was unintentionally about to bookend a month-ish long tour by attending both the first date (at Maryland Deathfest) and last date of the U.S. run of Japan’s doom masters, Church of Misery. For this, the final show, the supporting bands were The Atomic Bitchwax from New Jersey and Toke, minus their very recently axed drummer,…

[leenaevilin]

[Announcement] ミュージカル「Dance with Devils ~ Fermata~」(musical dance with devils ~fermata~)

you will be able to watch it @ 衛星劇場 on April 12th, 2020 (6pm) & April 25th, 2020 (11pm)

[recentanimenews]

SCARLET NEXUS TV Anime 2nd PV Introduces OP Theme by THE ORAL CIGARETTES

    The official website for the upcoming TV anime based on Bandai Namco's action-RPG Scarlet Nexus has posted an 80-second second PV to introduce its OP theme song "Red Criminal" by the four-member Japanese rock band THE ORAL CIGARETTES. The band also provides the theme song "Dream In Drive" for the game.

  The song will be digitally released on June 30, 2021. The band's vocalist/gutarlist Takuya Yamanaka says, "We're really happy to be able to sing the OP song for the anime following the game version of 'SCARLET NEXUS.' The song expresses Kasane and Yuito's struggle against their heroic fate, and the conflicts they feel in the midst of it. Please enjoy our music along with the world view of the anime."

    TV anime 2nd PV featuring the OP theme song "Red Criminal":

    Game's launch trailer featuring the theme song "Dream In Drive":

    The Sunrise-animated TV series is set to premiere in Japan on TOKYO MX, BS11, and Sun TV on July 1, 2021. Prior to the premiere in Japan, its first episode is now streamed on Funimation's official YouTube channel for a limited time.

      Synopsis:

  Solar calendar year 2020: grotesque organisms called Others have begun eating people. To take down this new enemy, the Other Suppression Force is formed. Saved by this elite team as a child, psychokinetic Yuito withstands the training to enlist. On the other hand, prodigy Kasane was scouted for her abilities. But Kasane’s dreams tell her strange things, dragging the two into an unavoidable fate. 

    Key visual:

    Main Staff:

  Director: Hiroyuki Nishimura (Gundam The Origin character designer)

Series Composition/Screenplay: Yoichi Kato, Toshizo Nemoto

Screenplay: Akiko Inoue

Character Design: Yuji Ito, Hiroyuki Nishimura

Art Director: Osamu Tayama

Color Design: Nagisa Abe

CG Line Director: Hitomi Ito

CG Modeling Director: Tatsuya Shimano

Director of Photography: Daichi Izeki

Editor: Masato Yoshitake

Music: Hiroyoshi Anazawa

Sound Director: Yayoi Tateishi

Original Story: Namco Bandai Entertainment

Planning and Anime Production: Sunrise

Production: SCARLET NEXUS Production Committee 

    Main Voice Cast:

  Suito Sumeragi: Junya Enoki

Kasane Randall: Asami Seto

Hanabi Ichijo: Tomomi Mineuchi 

Tsugumi Nazar: Marika Takano

Gemma Garrison: Ryota Takeuchi

Luka Travers: Chinatsu Akasaki

Siden Ritter: Kengo Kawanishi

Arashi Spring: Yui Horie

Kyouka Eden: Yumi Hara

Kagerou Dan: Daisuke Namikawa

Nagi Carman: Yuma Uchida

Naomi Randall: Asuka Nishi

    Source: TV anime "Scarlet Nexus" official website / Twitter, Funimation YouTube channel

  ©BNEI/SUNRISE

  By: Mikikazu Komatsu

[riffrelevant]

CHURCH OF MISERY Rare U.S. Headline Tour; Support From THE ATOMIC BITCHWAX, MONDO GENERATOR & TOKE

CHURCH OF MISERY Rare U.S. Headline Tour; Support From THE ATOMIC BITCHWAX, MONDO GENERATOR & TOKE

Article By: Pat ‘Riot’ Whitaker, Senior Writer/Journalist ‡ Edited By: Leanne Ridgeway, Owner/Chief Editor

In a stunning announcement, Japan’s ministers of stoner doom metal heaviness, CHURCH OF MISERY, this spring they will undertake a U.S. headlining tour! (more…)

[leenaevilin-birthday-calendar]

[July] J-Actors Birthday Calendar

♪ July 1st

◎  Furuie Hiroyuki 古家広之 1990

◎ Horikoshi Ryou 堀越涼 1984

♪ July 2nd

◎ Itakura Takeshi 板倉武志 1985

◎ Takano Akira 高野洸 1997

◎ Yoshitaka Shion 吉高志音 1999

♪ July 3rd

◎ Akaba Mio 紅葉美緒 1987

◎ Kaku Kento 賀来賢人 1989

◎ Minami Keisuke 南圭介 1985

♪ July 4th

◎

♪ July 5th

◎ Kosaka Ryoutarou 小坂涼太郎 1996

◎ Oomi Takuto 大見拓土 1994

◎ Uesugi Teru 上杉輝 1982

♪ July 6th

◎ Inoue Yoshio 井上芳雄 1979

◎ Kuroba Mario 黒羽麻璃央 1993

♪ July 7th

◎ Itou Yousuke 伊藤陽佑 1984

◎ Yashima Ryou 八島諒 1995

♪ July 8th

◎ Akiyama Shintarou 秋山真太郎 1982

◎ Suehara Takuma 末原拓馬 1985

♪ July 9th

◎ Furukawa Yuuta 古川雄大 1987

◎ Kishimoto Yuuta 岸本勇太 1993

◎ Masaki Kaoru 正木郁 1995

◎ Nagata Seiichiro 永田聖一朗 1998

◎ Yashiro Takuya 矢代卓也 1999

◎ Yoshida Tomokazu 吉田友一 1982

♪ July 10th

◎ Koizumi Koutarou 小泉孝太郎 1978

◎ Yazaki Hiroshi 矢崎広 1987

♪ July 11th

◎ Ayagiri Takuya 綾切拓也 1991

◎ Morimoto Ryouji 森本亮治 1982

◎ Murata Hisashi 村田恒 1993

◎ Murata Youjirou 村田洋二郎 1980

♪ July 12th

◎ Isaka Tatsuya 伊阪達也 1985

◎ Kageyama Tatsuya 影山達也 1992

◎ Onoda Ryuunosuke 小野田龍之介 1991

♪ July 13th

◎ Tsuji Ryoushirou 辻凌志朗 1993

♪ July 14th

◎

♪ July 15th

◎ Igarashi Keisuke 五十嵐啓輔 1987

♪ July 16th

◎ Mitani Kenta 三谷謙太 1994

◎ Ogasawara Ken 小笠原健 1986

♪ July 17th

◎ Hayashida Kouhei 林田航平 1988

♪ July 18th

◎ Murakawa Shouichi 村川翔一 1995

♪ July 19th

◎ Chiwata Yuuhei 千綿勇平 1991

◎ Saeki Daichi 佐伯大地 1990

♪ July 20th

◎ Fujito Yuuhi 藤戸佑飛 1994

◎ Katou Junichi 加藤潤一 1985

◎ Kujirai Kousuke 鯨井康介 1987

♪ July 21st

◎ Kudou Hiroki 工藤博樹 

◎ Sasamori Hiroki 笹���裕貴 1997

◎ Yoshimura Kazuhiro 吉村和紘 1983

♪ July 22nd

◎ Suzuki Mirai 鈴木身来 1993

♪ July 23rd

◎ Nishino Ryuuta 西野龍太

♪ July 24th

◎ Shiozawa Hidemasa 塩澤英真 1986

◎ Matsuzaki Yutaka 松崎裕 1978

♪ July 25th

◎ Chida Kyouhei 千田京平 1996

♪ July 26th

◎ Harano Masaaki 原野正章 1992

◎ Mizutani Atsushi 水谷あつし 1965

◎ Nakajima Takuto 中島拓人

♪ July 27th

◎ Miyagi Koudai 宮城紘大 1994

◎ Mori Ippei 森一平 1995

◎ Onaga Suguru ���長卓 1983

♪ July 28th

◎ Adachi Yuuto 安達勇人 1988

♪ July 29th

◎ Koga Ruito 古賀瑠 2004

♪ July 30th

◎ Mitsui Ryou 三井理陽 1997

◎ Uenobori Makoto 植野堀誠 1986

♪ July 31st

◎ Ono Kazuki 小野一貴 1990

[sambart93]

2018.03.19 Dance with Devils The Musical: Fermata [Part One Review!]

I have two tickets to see this play (I gotta see both endings, duh!)  and I’m seeing it for the second time on Wednesday but seriously you guys...this is definitely making my top 5 stages/musicals for the year! I HAVE to get this review/report out ASAP! And once I’ve seen it a second time, I’ll make a Part Two post which will be more coherent and more detailed perhaps? Or just has extra notes.. we’ll see. 

MAIN POINT 

THIS WAS AMAZING AND I NEED TO GET THAT OUT ASAP! 

I’m watching Patarillo today (Tuesday) between the two DWD shows but in all honesty, now I don’t give a shit about Patarillo! I just wanna watch DWD again!! Hurry up Wednesday!!!

Official Website here Official Twitter here Press Coverage 1, 2, 3, 4

CAST and CHARACTERS

Kaminaga Kousuke as Remu Takano Akira as Lindo Yamazaki Shougo as Urie Yoshioka Yu as Mage Yasukawa Junpei as Shiki Tanaka Ryuusei as Jek Naitou Daiki as Loewen Washio Noboru as Viv Kimata Hiroyuki as Grand Fukui Daisuke as Ell Kobayashi Tatsuyuki as Shida Fukushima Kaita as Kazura Kitagawa Naoya as Marius Kimotsuki Rui as Ritsuka Dancer: Imai Ryo, Tsuji Daiki, Kimura Atsuki, Osaka Sora, Ozeki Kousuke

*

I had a front FUCKING row for this tonight! I had THE center seat for this! IT WAS AmAZiNG! Pretty much the best 8800 yen I’ve every spent! Excluding that one front row seat in Ki ni Mesu Mama where Tomo held my hand.

*NON-SPOILER WILL BE POSTED AFTER SECOND VIEWING but guaranteed it’s atleast a 9/10 rating.

Jumping straight into spoilers!

STORY

So the story is still the bare basics, the devils coming to the human world to find Ritsuka and the Grimore and the vampires stealing Ritsuka BUT we have Marius who watches over and narrates this story (in fact he even says the 'who will you choose? Akuma or Exocist?' which is what used to just be written on the walls via projection mapping) and hints that history and this story will forever repeat itself and he has some connection with Loewen and Jek ad is able to visually manipulate Shiki which causes the story to change somewhat; Shiki ends up taking Jek's side which ends in Shiki and Mage fighting, Shiki is also manipulated into seeing his old angel friend who he brutally killed and murdered years ago and is the reason why he's so in love with breaking things. << As you can tell, we get a LOT more Shiki this time, so much Shiki story, scenes and development this time around. Also instead of Holland we have Urie's butlers so we get more of Urie's backstory and personality, which leads to Urie being more forward to Remu about liking Ritsuka and this causes a MASSIVE fight between them RIGHT at the end of the story -- it broke my heart! 

The ending I watched for my first show was the Remu ending.

That's pretty much all the major changes, so let's get down to the structure changes.

We actually spend a LONG time doing scenes before we get to the main opening theme song! We have Urie's butlers having daydream scenes of Urie and his relationship with Remu -- they do it via an over-the-top ridiculous comedy skit. And then we have Remu and Urie talking about Remu going to the human world by himself but Urie persuades Remu to let him go too; Mage and Shiki over hear and force their way into joining too. THEN we finally get to the opening theme song.

The story between Ritsuka starting school and Ritsuka being kidnapped is essentially the same 100%; we get their solo songs, we get them trying to talk to her in the school council, we get Remu saving her when her house is burnt down, we get Lindo returning from London and the students trying to figure out who he is etc. BUT we have extra scenes of Shiki and Ritsuka in this when she sees him painting and he goes to destroy it and she tries to stop him and they have a moment. Also Jek's song this time is a crowd pleaser that you're allowed to clap along to and squeal to. AND the freaking students have a song this time around that we can clap along to and is so much fun!

As previously mentioned we also get Shiki's backstory of when he was in heaven and he had a fellow angel friend called Ell but Shiki was drawn towards destroying stuff, so much so that eventually he turned on his friend and murders him and that's why he got kicked out of heaven.

*

OKAY now to me randomly flailing about everything! It's going to be VERY all over the place but as long as you know the basic story, I think you'll understand what I'm talking about:

During the scene when the students are talking about Lindo 'the new transfer student', the students reminisce about when they gave presents to Remu and it turns into a comedy skit where: The students drew the worst picture of Remu but it was during a comedy skit so Remu was fake happy about it and we had Mage behind him like 'it looks exactly like you!' and Remu replied 'STFU!' xD The drawing of Remu!!! Then Shiki came in and took the drawing and ripped it oh so slowly.

When the boys first meet Ritsuka, this time Urie was REALLY feeling up Ritsuka!! He held her and then rubbed his hand up her thigh really strongly and slowly and I was like 'OH MY!' 

When it's just Ritsuka and Shiki, and Shiki holds her close and goes pretty vampy on her with his mouth SO SO close to her neck. I love how after this scene, she goes to her brother but still has Shiki's paint brush in her hand. OHHHHH AND HOW FUCKING MUCH SHE TRIES TO PROTECT SHIKI MULTIPLE TIMES FROM EVERYONE ELSE (cos they're trying to beat him up and hurt him) AT ONE POINT!!! OMG IT WAS TOO PRECIOUS!!!

Also the scene with Shiki and the students and the knife! The students didn't know what to do with him so they tried to hold it but got scared so tried to put it in their pants but got spooked again, their reactions were amazing! Serious kudos to the students this time around! They were absolutely FABULOUS!

In fact in general, ALL the reactions and little movements we get from the rest of the characters when they're in the background and/or aren't the main focus of the scene at that point were all amazing! We got such good moments; like the students (above) and we had things like Mage and Shiki holding hands at one point, and we had Shiki smiling so evilly when things weren't going well for Remu, 

AND!! Marius in general - he's never really the center stage, he's always in his little bubble up on the top left so he is constantly giving the best and most interesting reactions and Naoya does this thing of evilly licking his lips multiple times and I enjoyed it a BIT too much xD But, Naoya is ridiculously thin. You could see all the muscles in his legs and arms (like even the muscles between his wrist and elbow) and his elbows have like THREE points because he's just so thin. I swear he's currently medically anorexic which hurts my heart! I hope he puts weight on soon.

And just... ALL the little things were amazing.

I feel like this time around is SO MUCH DARKER. Especialy towards the end when we have Shiki and Jek pairing up, we also have Shiki almost killing Ritsuka at one point, we also have (I think it was in the anime though) Jek slitting Ritsuka's wrists, we have Shiki and Ell fighting, Shiki and Mage, Remu and Urie fighting. Especially the DAMN ending where it looks like Ritsuka and Remu are about to walk off and Urie is like 'fight me here RIGHT now. I'm not letting you have Ritsuka' and they beat the crap out of each other... goddammit. 

Which also leads to my next point; I CRIED LIKE A BITCH! Seeing Shiki and Mage and then the twist with Remu and Urie, I was in STREAMS of tears! I was an absolute wreck! I also burst out into tears when they did the slow version of the theme song ♡ It's so good!

Personally I feel that ALL the crap they added and change and stuff was just PERFECTION!

Ohh! We also had new songs from Lindo, Jek, the Students and I think one more. I liked all the new songs! And I really liked Lindo's new song and he dances with Ritsuka in it which is so cute! Also he sang this song during the live but it was with Loewen and seeing them do the dance together was ADORABLE! During Lindo and Loewen's duet and Loewen was all curled up and barking and it was adorable! AND during the students' song Lindo was so bullied by them because they didn't know it was him and he tried to join in with their dancing and stuff and they just pushed him out xD

OOHHH BUT I HAVE TO TELL YOU!!!!!! YOSHIOKA AND JP ACCIDENTALLY KISSED AT ONE POINT!!! AND YOSHIOKA COULDN'T GET BACK INTO CHARACTER COS HE WAS SHOCKED AND THE AUDIENCE WERE SQUEALING. JP managed to keep his cool and Yoshioka was taken completely off balance xD It happened just after Mage's solo and Shiki walks in after Ritsuka has run away from Mage.

The new Urie is so damn PRETTY!! He’s the best singer for Urie they’ve had I think. And he reminded me of Ino Hiroki a tad. While I am still Kamisato bias for Urie, this guy did a damn good job and I wouldn't mind him staying as Urie from now on. 

They kept Ritsuka as a male ballet dancer and he did a really good job! He was cute too (not Miura Hiroki cute but cute enough) He was a bit taller than what we've had before - so I kinda giggled when the rest of the boys got close to 'her' because I think she was taller than most of them xD And because he's over 20 (the dancer) this time, it explained why Urie, Shiki and such got a lot more touchy feely with Ritsuka this time around. I feel like they also they weren't as bothered this time about hiding Ritsuka's face because (I assume) they know the audience likes that it's a guy playing Ritsuka and we all want to know what he looks like and such. BUT it could just be because I was right at the front so the lighting and stuff didn't hide any of her face from me? We'll see.

It was either Remu and Urie or Mage and Shiki but either Urie/Shiki smiled and starred at the other for a long time and I was getting just such BL feels.... It was Shiki and Mage! I remembered now! Shiki was just starring down Mage and I was getting a little...hot.

I am so thankful that the production and director listened to us fans this time and gave us so much more Shiki ♡ it was the best!

Also it did not feel like 2 and a half hours which proves it was a damn good play! If something is so enjoyable that you don't feel it, you know they did good.

I feel like Kaminaga is SUCH an UNDERATED actor and I too always underrated him; like I LOVE whatever the hell I see him in but the rest of the time, I just forget about him! But he's such a good Remu!!!!!!!! He's so great!!!! He needs more love!!!

The girls behind me were like ‘woah this is our fifth time! But we haven’t been THIS close (second row) yet! Now I’m super nervous’ xD

ANNND! Can we discuss AKIRA’S THIGHS?!?!?! Can we just?! Because they are SO NICE! They fit his jeans so TIGHTLY! They looked great!!!

Seriously, the ending fucked me up when Urie started turning on Remu!!! Holy crap! And it sounded like the actor really smacked his leg on the floor so I think his hobbling was real for a good few minutes.

OMG and just before Jek's solo, Ryuusei came trampsing on stage like 'Today's magic word it Maji Manji!' and then he went on talking about something like being a high school girl xD I wouldn't put it past him.

I LOVE how Urie's butler Viv and his guard Grand dressed up in school uniforms and had these cute buns and hairstyles for their cover.

During the live Urie gave his rose to Mage and he was like 'what do I want this for?!' and then Shiki ran after him and was like 'give it to me!' but Mage refused and offered to a girl in the front row instead xD Seriously, JP and Yoshioka are such good friends and it just SHINES through whenever I watch DWD. 

There's a moment where Urie is constantly whipping and Mage is constantly punching Shiki.... Urie with a whip is not good o_O but really it is xD

I really really liked the new set! Center back we had this steampunk like bell and we had cogs around the rest of the stage and in the center we had an arrangement of steps around in a circle and the circle platform would spin around. I really liked it!

OHHH! Remu's Sauron eye of a father made several appearances. AND I spend the first few minutes of the stage laughing at Remu because I remembered just HOW MUCH I hate his shoes.

Also after the fight scene, I could hear Urie's actor ripping the velcro straps off his costume the MOMENT he stepped off stage xD It was pretty loud actually, especially because it was a non-music quiet scene on stage.

ALSO I swear to god one of the music tracks is very similar to LoTR music! I heard it and I was like 'where are my elves at?!' it sounds so familiar! 

In general, all the DWD music is great! I don't think I have a song or soundtrack number I don't like...

Final note: I will fight ANYONE who’s all for Ritsuka/Lindo instead of Ritsuka/Remu!!! << But watched me watch the other ending on Wednesday and I fall for Lindo/Ritsuka xD

*

OH! After the show, there were kankeishas in front of me when I was walking out;

Guy: so this is a series? What number are we at? Girl: this was the second one

It took ALL my energy not to butt in and correct her. It is the THIRD one ACTUALLY!

*

GOODS

*

And I’m done! Now to go and see Patarillo now... but I just want tomorrow already!

[crazyasianlove]

HiGH&LOW THE MOVIE 3 FINAL MISSION (JM, 2017) (Sub. Esp)

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Título: HIGH&LOW The Movie 3 Final Mission País: Japón Género: Acción Duración: 118 min. Fecha de estreno: 11 de noviembre, 2017 Dirección: Kubo Shigeaki, Nakakuki Tsuyoshi Guion: Hiranuma Norihisa, Watanabe Kei, Kamijo Daisuke Producción: Ueno Hiroyuki, Fujimura Naoto, Nakabayashi Chikako SINOPSIS A fin de ocultar la pasada corrupción del gobierno, el grupo Kuryu sigue un plan para destruir uan calle y construir un casino. Para detener al grupo Kuryu, los miembros de SWORD comienzan a moverse. CAST ASOCIACIÓN SANNOH Iwata Takanori como Cobra Suzuki Nobuyuki como Yamato Machida Keita como Noboru Yamashita Kenjiro como Dan Sato Kanta como Tetsu Sato Taiki como Chiharu Yagi Masayasu como Ijuin Kabuto Iwaya Shogo como Ken Yamamoto Shogo como Hikaru WHITE RASCALS Keiji como Rocky Endo Yuya como Koo Inaba Yu como Kizzy Yanagi Shuntaro como Kaito Nishimura Ikki como Heidi Hirose Tomoki como Marco Nisikawa Shunsuke como Lassie Matsuda Ryo como Cosette Kyan Yutaka como Bito Darvish Kenji como Shimura INSTITUTO OYA Yamada Yuki como Murayama Yoshiki Suzuki Takayuki como Furuya Hideto Ichinose Wataru como Seki Kotaro Aoki Ken como Nakakuki Kiyohara Sho como Nakabayashi Jinnai Syo como Nakazono RUDE BOYS Kubota Masataka como Smoky Sano Reo como Takeshi Zen como P Sano Gaku como Yu Fujii Karen como Lala DARUMA IKKA Hayashi Kento como Hyuga Norihisa Abe Ryohei como Sakyo Ozawa Yuta como Kato Shu Mizuno Masaru como Futa Tanaka Shunsuke como Raita Moriya Koji como Agyo Izawa Yuki como Ungyo DOUBT Nakamura Aoi como Hayashi Ranmaru Akiyama Shintaro como Takano Takeda Kohei como Hirai MIGHTY WARRIORS Elly como Ice Oya Kana como Sarah Nogae Shuhei como Pearl Shirahama Alan como Bernie Anarchy como 9 Likiya como Diddy Sukezane Kiki como Dixie PRISON GANG Naoto como Jesse Sekiguchi Mandy como Pho Iwanaga Joey como Brown Nakatani Taro como Mocai Jay como Nakamon Takeru como Miou Kido Yasuhiro como Akune (EX)MUGEN Akira como Kohaku Aoyagi Sho como Tsukumo HERMANOS AMAMIYA Takahiro como Amamiya Masaki Tosaka Hiroomi como Amamiya Hiroto GRUPO KURYU Tsugawa Masahiko como Kuze Ryushin Iwaki Koichi como Kurosaki Kimitatsu Kishitani Goro como Zenshin Yoshitatsu Kato Masaya como Katsunari Ryuichiro Sasano Takashi como Ueno Ryuhei Takashima Masahiro como Minamoto Ryukai Kinoshita Youka como Fujimori Tatsuo Nakamura Tatsuya como Iemura Tatsumi Saotome Taichi como Ryu Tatsuhito Kobayashi Naoki como Kuki Genji Kenchi como Nikaido Onozuka Hayato como Kirinji Takeda Kozo como Yoshida Kagetora Kuroishi Takahiro como Udaka Shinichi Yuki Jutta como Oba Kunimitsu Shogen como Okido Ken Shiroishi Tomoya como Eto Araki Hideyuki como Fukuda Watanabe Kohei como Yagi ICHIGO-MILK Kaede como Shiba Sato Harumi como Oshiage Yamaguchi Nonoka como Nonoriki Kido Airi como Ichikawa OTROS Fujii Shuka como Naomi Bando Nozomi como Ijuin Nika Nakai Noemie como Furuno Suzuki Rio como Eri Horibe Keisuke como Natano Eichi Hasegawa Hatsunori como Shinohara Toyohara Kosuke como Saigo Ikeue Kohei como Kikuchi

TRÁILER: https://www.youtube.com/watch?v=Jq7mAAjn8nw

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bFGF Expression by Mast cells in Osteoarthritis Patients

New Post has been published on https://depression-md.com/bfgf-expression-by-mast-cells-in-osteoarthritis-patients/

bFGF Expression by Mast cells in Osteoarthritis Patients

Ken Takata, Kentaro Uchida, Shotaro Takano, Manabu Mukai, Gen Inoue, Hiroyuki Sekiguchi, Jun Aikawa, Masayuki Miyagi, Dai Iwase, Masashi Takaso

Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, 252-0374, Japan

Correspondence: Kentaro Uchida Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-Ku Kitasato, Sagamihara City, Kanagawa, 252-0374, Japan Tel/Fax +81-042-778-9217 Email [email protected]

Purpose: Obesity is associated with the risk of developing knee osteoarthritis (KOA). Furthermore, synovial basic fibroblast growth factor (bFGF) is linked to the severity of KOA. We previously demonstrated that bFGF and mast cell (MC) marker expression were elevated in the synovial tissues (ST) of KOA patients with obesity. However, it remains unclear whether MCs contribute to bFGF expression and regulation. Patients and Methods: Radiographically diagnosed KOA patients (n=249) were assigned to groups based on the body mass index (BMI) classifications used by the World Health Organization: normal-weight (NW, BMI 2, n=95), overweight (OW, BMI ≥ 25 and 2, n=45). BFGF expression in the ST was examined using quantitative polymerase chain reaction and compared across the BMI groups. Additionally, BFGF and interleukin (IL) 13 expression were examined in freshly extracted MC-rich (THY-1-, CD3-, CD14-, and CD19-) and MC-poor (THY-1+, CD3+, CD14+, or CD19+) fractions from ST. Moreover, regulation of BFGF expression by IL-13 was studied in CD14-negative (fibroblast-rich) and CD14-positive (M&phis;-rich) and cells in culture. Results: BFGF expression was significantly higher in OB than in NW patients. Furthermore, although IL13 was significantly higher in the MC-rich than the MC-poor fraction, BFGF expression was comparable. Recombinant human IL-13 stimulated expression of BFGF in synovial fibroblast cells. Conclusion: BFGF expression is higher in the ST of KOA patients with obesity. Increased numbers of MCs may contribute to the elevated BFGF expression through IL-13 in KOA patients with obesity.

Introduction

Obesity has been shown to increase the prevalence and development of osteoarthritis (OA) in weight-bearing as well as non-weight-bearing joints.1,2 This suggests that other factors apart from mechanical loading may explain the association between obesity and OA. Such other factors may also contribute to OA pathology. However, these factors remain elusive.

Basic fibroblast growth factor (bFGF) regulates cell proliferation and contributes to the matrix metalloproteinase production and breakdown of the extracellular matrix in articular cartilage.3 Furthermore, bFGF contributes to the pathology of joint destruction,4 with bFGF levels in synovial fluid having been shown to be associated with radiographic severity of knee OA (KOA).5 In our previous report, we found that BFGF mRNA expression was increased in the synovial tissues (ST) of KOA patients with obesity.6 However, the mechanisms underlying the rise in bFGF in patients with obesity remain unclear.

Mast cells (MCs) are present in the ST, and are elevated in patients with rheumatoid arthritis (RA)7,8 and OA,9–11 where they are suggested to play a pivotal role in both acute and chronic inflammation. Recent evidence indicates a possible link between MCs and KOA severity.10 bFGF is among a range of growth factors generated by MCs under inflammatory conditions.12,13 We previously reported that BFGF expression was correlated with the MC marker expression.6 However, bFGF expression in MCs remains to be studied.

Interleukin (IL)-13 is an MC-secreted cytokine.14–16 Increased IL-13 levels are observed in the synovial fluid of patients with KOA and rheumatoid arthritis.17,18 IL-13 level in synovial fluid is correlated with OA pain and function.19 Interestingly, IL-13 has been shown to regulate bFGF in airway smooth muscle cells.20 We hypothesized that MCs express IL-13 and that IL-13 regulates bFGF in the ST of KOA patients.

We investigated BFGF and IL13 expressions in MCs, and the effect of IL-13 on BFGF expression in KOA patients.

Patients and Methods

Total knee arthroplasty (TKA) was performed on all participating subjects at our hospital. A total of 249 ST specimens were harvested from patients diagnosed with radiographic KOA during TKA. A small section of each specimen was immediately frozen in liquid nitrogen and placed at −80°C until RNA extraction. Specimens taken from 10 patients were subjected to experiments aimed at determining BFGF expression in MCs, and samples taken from 8 patients to experiments aimed at examining whether IL-13 regulates BFGF expression.

The study protocol received approval from Kitasato University Hospital institutional Ethics Review Board (approval number: KMEO B19–259). All participants provided written informed consent to participate and for removal of their synovial tissue the day prior to surgery. This study complied with the principles of the Declaration of Helsinki.

Patients were assigned to groups based on the body mass index (BMI) classifications used by the World Health Organization: normal-weight (BMI <25 kg/m2, n=95), overweight (BMI ≥25 and <30, n=109) and obese (≥30 kg/m2, n=45). The patients’ clinical characteristics are summarized in Table 1 by BMI group. MC marker (CD117, CD203c, TPSB2 (β-tryptase)) and BFGF expression in the ST were compared across the BMI groups using RT-PCR.

Table 1 Patients’ Clinical Characteristics

qPCR

Details of RNA extraction, cDNA synthesis and qPCR using SYBR Green are published elsewhere.8,21 qPCR primer sequences are shown in Table 2. All gene expression was normalized to that of GAPDH.

Table 2 Primer Sequences

Expression of BFGF and IL13 in MCs

Cells isolated from synovial tissue were separated into MC-rich and MC-poor fractions as described previously,21 and examined for BFGF and IL13 expression. To digest ST, samples were immediately immersed in collagenase solution at 37°C for 2 h. Extracted cells reacted with the following antibodies for 30 minutes at 4°C: biotin-conjugated anti-THY-1 (fibroblast marker), anti-CD3 (T cell marker), anti-CD14 (Mφ marker), and anti-CD19 (B cell marker). The cells were subsequently washed twice with Hanks’ balanced salt solution, and exposed to streptavidin-conjugated magnetic particles (BD Biosciences, CA, USA). A magnetic separation system (BD Biosciences) was used to separate the cells into negative (MC-rich; THY-1-, CD3-, CD14-, and CD19-) and positive (MC-poor; THY-1+, CD3+, CD14+, or CD19+) fractions. qPCR was used to determine the BFGF and IL13 expression in negative (MC-rich) and positive fractions.

Effect of IL-13 on Synovial Fibroblasts and Mφ

Synovial fibroblasts and Mφ were isolated from ST samples harvested from 8 KOA patients to study the role of IL-13 in the synovium. CD14-positive (Mφ-rich) and CD14-negative (fibroblast-rich) cells were separated using the magnetic separation technique described in a previous protocol.22 CD14-negative and CD14-positive cells were seeded in α-MEM in six-well plates and cultured for 7 days. Thereafter, the cells were exposed to vehicle (serum-free media) or 100 ng/mL of recombinant human IL-13 (rh-IL13; BioLegend) for 6 hours. The IL-13 concentration was chosen based on a protocol reported previously.20 Following stimulation, qPCR was conducted to measure BFGF expression.

Statistical Analyses

All analyses were conducted based on a pre-specified statistical analysis plan. All analyses were performed using SPSS 25.0. Continuous variables were compared between two groups using the Wilcoxon signed-rank test and among three groups using the Kruskal–Wallis test followed by the Mann–Whitney U-test using Bonferroni correction. Categorical variables were compared using the Fisher’s exact test. P<0.05 was indicative of statistical significance.

Results

Clinical Characteristics by BMI

OB patients were significantly younger than NW and OW patients (Table 1). The proportion of male to female patients (P=0.099) and those with Kellgren and Lawrence grade 3 and 4 (P=0.634) was comparable across the BMI groups (Table 1).

Expression of Synovial MC Markers (CD117, CD203c, TPSB2) and BFGF by BMI

OB patients had significantly higher levels of CD117 than NW patients (P=0.011), while NW- and OW patients had comparable levels (P=0.535) (Figure 1A). OB patients also had significantly higher levels of CD203c than NW patients (P=0.027) but comparable levels to OW patients (P=0.161) (Figure 1B). Likewise, OB patients had significantly higher levels of TPSB2 than NW patients (P=0.002) but comparable levels to OW patients (P=0.247) (Figure 1C).

Figure 1 CD117 (A), CD203c (B), TPSB2 (C), and BFGF (D) mRNA expression in the synovium of normal, overweight, and obese patients with knee osteoarthritis. *P<0.05. P values are indicated.

Similarly, OB patients had significantly higher levels of BFGF expression than NW patients (P=0.020) but comparable levels to OW patients (P=0.283) (Figure 1D).

Correlation Between MC Markers and BFGF Expression

Since our results indicated that KOA patients with obesity had higher expression levels of MC markers (CD117, CD203c, TPSB2) and BFGF, we next investigated the correlation between MC marker and BFGF expression. BFGF expression was significantly correlated with CD117 (ρ=0.273, P<0.001; Figure 2A) CD203c (ρ=0.432, P<0.001; Figure 2B) and TPSB2 (ρ=0.164, P<0.001; Figure 2C) levels.

Figure 2 Correlation between expression of BFGF and MC markers. Correlation between expression of BFGF and CD117 (A), CD203c (B), and TPSB2 (C).

MC Markers, BFGF and IL13 in MCs

Next, we measured the expression of MC markers (CD117, CD203c, TPSB2), BFGF and IL13 in MCs in MC-rich and MC-poor fractions isolated from ST specimens of KOA patients. The MC-rich fraction expressed significantly higher levels of MC markers (P=0.005; Figure 3A–C) and IL13 (P=0.009; Figure 3D) than the MC-poor fraction. Meanwhile, MC-rich and MC-poor fractions expressed comparable levels of BFGF (P=0.285; Figure 3E).

Figure 3 Expression of MC markers, BFGF and IL13 in mast cells. CD117 (A), CD203c (B), TPSB2 (C), IL13 (D), and BFGF (E) expression levels in mast cell (MC)-rich (THY-1-CD3-CD14-CD19-) and MC-poor (THY-1+, CD3+, CD14+, or CD19+) fractions. *P<0.05. P values are indicated.

Effect of IL-13 on BFGF Expression in Synovial Fibroblasts and Mφ

Given that BFGF expression was comparable between MC-poor and MC-rich fractions, we next studied the effect of rh-IL13 on BFGF expression in fibroblast- and Mφ-rich fractions isolated from the ST of KOA patients. Exposure to rh-IL13 induced BFGF expression in synovial fibroblast-rich fractions (P=0.043; Figure 4A) but not Mφ-rich fractions (P=0.093; Figure 4B) at 6 h following stimulation.

Figure 4 Effect of IL-13 on BFGF expression in synovial fibroblasts and Mφ. BFGF expression in CD14-negative (fibroblast-rich) (A) and CD14-positive (Mφ-rich) (B) fractions at 6 h after stimulation with vehicle or IL-13. *P<0.05. P values are indicated.

Discussion

Obesity alters local and systemic bFGF levels. Furthermore, high levels of plasma FGF2 have been shown to be correlated with fat mass in Han Chinese populations.23 In our previous study, we reported that KOA patients with obesity had higher expression of synovial bFGF and MC markers than normal-weight patients with OA.6 Consistent with our previous results, we found that KOA patients with obesity had elevated bFGF and MC marker expression in the present study. Accumulating evidence indicates that bFGF contributes to OA pathology and cartilage destruction. bFGF stimulates the expression of MMP-13 expression in human articular chondrocytes.24 Thus, elevation of bFGF may contribute to OA pathology in KOA patients with obesity.

Immunohistochemical studies have reported increased bFGF staining in hyperplastic lining synoviocytes, and vascular cells in ST derived from rheumatoid arthritis patients.7 To investigate the potential role of MCs in bFGF expression, we measured bFGF expression in MCs isolated from osteoarthritic ST. However, we found no differences between MC and non-MC populations. Thus, although MCs express bFGF, whether MCs contribute to the elevation of bFGF in the osteoarthritic ST remains unclear.

Previous studies have reported that IL-13 is released by several immune cells including T cells,25 MCs,15,26 eosinophils,27 and basophils.28 We found that MCs expressed higher IL-13 levels than non-MC populations. In addition, IL-13 stimulated BFGF expression in synovial fibroblasts. Thus, increased MC numbers may induce BFGF expression in OA pathology by activating synovial fibroblasts in KOA patients with obesity.

There were main two limitations in the present study. First, we lack data on non-OA patients. Second, we did not examine protein levels.

In conclusion, KOA patients with obesity showed higher BFGF expression than normal- and overweight patients. Increased MC numbers may contribute to the elevation of BFGF expression through IL-13 in KOA patients with obesity.

Acknowledgments

This investigation was supported by Grant-in-Aid for Early-Career Scientists Grant No. 20K18073.

Disclosure

The authors report no conflicts of interest regarding this work.

References

1. Grotle M, Hagen KB, Natvig B, Dahl FA, Kvien TK. Obesity and osteoarthritis in knee, hip and/or hand: an epidemiological study in the general population with 10 years follow-up. BMC Musculoskelet Disord. 2008;9:132. doi:10.1186/1471-2474-9-132

2. Oliveria SA, Felson DT, Cirillo PA, Reed JI, Walker AM. Body weight, body mass index, and incident symptomatic osteoarthritis of the hand, hip, and knee. Epidemiology. 1999;10(2):161–166. doi:10.1097/00001648-199903000-00013

3. Im HJ, Muddasani P, Natarajan V, et al. Basic fibroblast growth factor stimulateSTatrix metalloproteinase-13 via the molecular cross-talk between the mitogen-activated protein kinases and protein kinase Cdelta pathways in human adult articular chondrocytes. J Biol Chem. 2007;282(15):11110–11121. doi:10.1074/jbc.M609040200

4. Li X, Ellman MB, Kroin JS, et al. Species-specific biological effects of FGF-2 in articular cartilage: implication for distinct roles within the FGF receptor family. J Cell Biochem. 2012;113(7):2532–2542. doi:10.1002/jcb.24129

5. Honsawek S, Yuktanandana P, Tanavalee A, Saetan N, Anomasiri W, Parkpian V. Correlation between plaSTa and synovial fluid basic fibroblast growth factor with radiographic severity in primary knee osteoarthritis. Int Orthop. 2012;36(5):981–985. doi:10.1007/s00264-011-1435-z

6. Uchida K, Takano S, Inoue G, et al. Increase in mast cell marker expression in the synovium of obese patients with osteoarthritis of the knee. DiabeteSTetab Syndr Obes. 2019;12:377–382. doi:10.2147/DMSO.S201523

7. Ramirez J, Celis R, Usategui A, et al. Immunopathologic characterization of ultrasound-defined synovitis in rheumatoid arthritis patients in clinical remission. Arthritis Res Ther. 2016;18:74. doi:10.1186/s13075-016-0970-9

8. Rivellese F, Rossi FW, Galdiero MR, Pitzalis C, de Paulis A. Mast Cells in Early Rheumatoid Arthritis. Int J Mol Sci. 2019;20(8):2040. doi:10.3390/ijms20082040

9. Buckley MG, Gallagher PJ, Walls AF. Mast cell subpopulations in the synovial tissue of patients with osteoarthritis: selective increase in numbers of tryptase-positive, chymase-negative mast cells. J Pathol. 1998;186(1):67–74. doi:10.1002/(SICI)1096-9896(199809)186:1<67::AID-PATH132>3.0.CO;2-D

10. de Lange-brokaar BJ, Kloppenburg M, Andersen SN, et al. Characterization of synovial mast cells in knee osteoarthritis: association with clinical parameters. Osteoarthritis Cartilage. 2016;24(4):664–671. doi:10.1016/j.joca.2015.11.011

11. Dean G, Hoyland JA, Denton J, Donn RP, Freemont AJ. Mast cells in the synovium and synovial fluid in osteoarthritis. Br J Rheumatol. 1993;32(8):671–675. doi:10.1093/rheumatology/32.8.671

12. Inoue Y, King TE, Tinkle SS, Dockstader K, Newman LS. Human mast cell basic fibroblast growth factor in pulmonary fibrotic disorders. Am J Pathol. 1996;149(6):2037–2054.

13. Qu Z, Liebler JM, Power STR, et al. Mast cells are a major source of basic fibroblast growth factor in chronic inflammation and cutaneous hemangioma. Am J Pathol. 1995;147(3):564–573.

14. Cameron L, Hamid Q, Wright E, et al. Local synthesis of epsilon germline gene transcripts, IL-4, and IL-13 in allergic nasal mucosa after ex vivo allergen exposure. J Allergy Clin Immunol. 2000;106(1 Pt 1):46–52. doi:10.1067/mai.2000.107398

15. McLeod JJ, Baker B, Ryan JJ. Mast cell production and response to IL-4 and IL-13. Cytokine. 2015;75(1):57–61. doi:10.1016/j.cyto.2015.05.019

16. Pawankar R, Okuda M, Yssel H, Okumura K, Ra C. Nasal mast cells in perennial allergic rhinitics exhibit increased expression of the Fc epsilonRI, CD40L, IL-4, and IL-13, and can induce IgE synthesis in B cells. J Clin Invest. 1997;99(7):1492–1499. doi:10.1172/JCI119311

17. Isomaki P, Luukkainen R, Toivanen P, Punnonen J. The presence of interleukin-13 in rheumatoid synovium and its antiinflammatory effects on synovial fluid Mφ from patients with rheumatoid arthritis. Arthritis Rheum. 1996;39(10):1693–1702. doi:10.1002/art.1780391012

18. Moue T, Tajika Y, Ishikawa S, et al. Influence of IL13 on Periostin Secretion by Synoviocytes in Osteoarthritis. Vivo. 2017;31(1):79–85. doi:10.21873/invivo.11028

19. Nees TA, Rosshirt N, Zhang JA, et al. Synovial Cytokines Significantly Correlate with Osteoarthritis-Related Knee Pain and Disability: inflammatory Mediators of Potential Clinical Relevance. J Clin Med. 2019;8(9):1343. doi:10.3390/jcm8091343

20. Lee JH, Kaminski N, Dolganov G, et al. Interleukin-13 induces dramatically different transcriptional programs in three human airway cell types. Am J Respir Cell Mol Biol. 2001;25(4):474–485. doi:10.1165/ajrcmb.25.4.4522

21. Noordenbos T, Yeremenko N, Gofita I, et al. Interleukin-17-positive mast cells contribute to synovial inflammation in spondylarthritis. Arthritis Rheum. 2012;64(1):99–109. doi:10.1002/art.33396

22. Takano S, Uchida K, Miyagi M, et al. Nerve Growth Factor Regulation by TNF-alpha and IL-1beta in Synovial Mφ and Fibroblasts in Osteoarthritic Mice. J Immunol Res. 2016;2016:5706359. doi:10.1155/2016/5706359

23. Hao RH, Guo Y, Dong SS, et al. Associations of PlaSTa FGF2 Levels and PolymorphiSTs in the FGF2 Gene with Obesity Phenotypes in Han Chinese Population. Sci Rep. 2016;6:19868. doi:10.1038/srep19868

24. Im HJ, Sharrocks AD, Lin X, et al. Basic fibroblast growth factor induceSTatrix metalloproteinase-13 via ERK MAP kinase-altered phosphorylation and sumoylation of Elk-1 in human adult articular chondrocytes. Open Access Rheumatol. 2009;1:151–161. doi:10.2147/oarrr.s7527

25. Corry DB. IL-13 in allergy: home at last. Curr Opin Immunol. 1999;11(6):610–614.

26. Burd PR, Thompson WC, Max EE, Mills FC. Activated mast cells produce interleukin 13. J Exp Med. 1995;181(4):1373–1380. doi:10.1084/jem.181.4.1373

27. Schmid-Grendelmeier P, Altznauer F, Fischer B, et al. Eosinophils express functional IL-13 in eosinophilic inflammatory diseases. J Immunol. 2002;169(2):1021–1027. doi:10.4049/jimmunol.169.2.1021

28. Li H, Sim TC, Alam R. IL-13 released by and localized in human basophils. J Immunol. 1996;156(12):4833–4838.

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