#dgm x ml
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What is the Function of TwinSpin in Buffy Coat Preparation?
You can go to our website for more details. Use our products to experience the distinction for yourself. Start using our density gradient centrifugation products for cell separation right away!
A buffy coat is an accumulation of platelets and white blood cells (WBCs). We'll discuss how TwinSpin helps in the preparation of a buffy coat.
A sample of peripheral whole blood contains less than 1% white blood cells (WBCs) and platelets. When scientists spin the blood sample in a centrifuge, these WBCs and platelets combine to form their own layer that is suspended between the red blood cells (RBCs) and supernatant plasma. This thin layer is referred to as a buffy coat because of its color (yellowish to brownish).
We will also find out how TwinSpin density gradient centrifugation tubes are used in buffy coat extraction.
A Brief On TwinSpin Tubes
Spin Medium can be used to separate peripheral blood mononuclear cells (PBMC) from bone marrow and whole blood into TwinSpin centrifuge tubes. A standard 15-inch tire and an inner tube make up the TwinSpin. The open bottom of the inner tube is submerged in the density gradient medium (DGM).
Instructions for Using the TwinSpin Tube
1. Ensure that the (diluted blood) sample, the TwinSpin, and the centrifuge are all at room temperature.
1.1 Check that the inner tube is partially filled and immersed in the DGM. If not, maintain the vertical position while rotating the TwinSpin device.
1.2 Take out and throw away the transport stopper.
2. Include Sample Material
2.1 Pipette the sample material on top of the DGM in the inner tube by tilting the TwinSpin 45° and allowing the sample to run down the inside of the dropper.
2.2 Push the elastic cap firmly into the opening to close the TwinSpin. PluriSpin PLT Depletion can be used to reduce platelet contamination.
3. Spin
3.1 Centrifuge at 800 x g for 20 minutes at room temperature in a swing bucket rotor, brake off. If the blood is more than 4 hours old, centrifuge it for 30 minutes at 1000g.
4. Collect
4.1 Remove the inner cell separation tube by unscrewing it.
4.2 Push the elastic cup down to collect cells in the opaque layer in a new tube.
5 Wash (if necessary)
5.1 Add wash buffer to the reaction tube.
5.2 Cells are spun down for 10 minutes with 300 x g at 4 °C (no or small break).
5.3 Remove the supernatant and set the reaction tube on the table for 20 seconds.
Excess wash buffer will flow down the tube wall and accumulate at the bottom. Aspirate the majority of the liquid above the pellet. The liquid will appear foggy because it contains mostly platelets; aspiration will improve the washing result.
5.5 Carefully pipette the pellet with 1 ml of wash buffer.
5.6 Re-do steps 5.1–5.4.
5.7 Refill the pellet to the desired volume.
Preparation of buffy coat from scratch
Buffy coats can be prepared in two different ways. To separate the whole blood into red cells, plasma, and buffy coat, the whole blood donation is centrifuged on the one hand. The second option is to filter the blood and keep the leukocytes from circulating.
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DGM X ML AU - SUPERHERO NAMES
Guess who’s back with more?? Me obviously! So i did after start writing the first chapter to this fic and midway through writing and drafting and outlining i realized that i didn’t come up with names for our miraculous holders (holy hell im a mess i should have thought that earlier) so unless anyone else has like cooler suggestions these are everyones current persona names that ill be using. @faeriexqueen @yugirl-with-dragons @kitty-ray (i know this au is all i talk about with you three but listen i have a mighty need okay)
Lavi - Pyraxis
Pyraxis is a rough translation to ‘line of fire’ and with Lavi being a chemist pun nerd, this was a name that he figured would be cool. After all, there’s only so many game of thrones references that he could handle.
Kanda - Tora
In Japanese, the way to say tiger is tora. Kanda isn’t very creative when it came to names and he got annoyed with the media calling him Tigger the Hero so he went with the easy route and gave himself a persona name in his native language.
Allen - Salem
Allen didn’t have much of a childhood being stuck with Cross Marian for a good portion of it. However, the small time that he was with Mana Walker, Allen remembered watching Sabrina the Teenage Witch and how much he loved the witch cat Salem. So of course when he got the black miraculous, he decided to go with it.
Miranda - Lady Age
Miranda chose this name due to her powers being relative to time. She also went with age since she wanted to sound just a tad bit mysterious and also just a little sophisticated.
Lenalee - Sky Niăo
Lenalee’s superhero name comes from a mixture of her current language of english and her native language of Mandarin. Lenalee embraces her newfound culture in the u.k. but doesn’t forget her roots in China.
Alma - Kumiho
Before getting to london, Alma was in Korea for awhile doing his own hero thing before following and tracking the enemy down to Western Europe. In Korea, the citizens there gave Alma the name Kumiho, a fox like creature in korean mythology. Alma felt that it was appropriate and so he kept it.
Cross - Candid Keeper
A name given to him as a joke by Tiedoll, Cross Marian thought it rather suited him well since he’s not very interested in the war of the miraculous. He likes to keep things on the downlow, which is why he travels all the time since he’s aware that the Earl is searching for the miraculouses.
Tiedoll - Gala-Pant
Tiedoll is one of the oldest members to posses a miraculous and was also the one to distribute the magic items to each of their current wielders. Since his miraculous armor resembles that of a galapagos tortoise and being the only with the knowledge of the miraculous’ origins and their true potential, he took the name Gala-Pant coming from the combination Galapagos and Pantomath.
Krory - Paladin Nyx
To be fair, Krory didn’t come up with this one. His kwami Wally decided that Krory’s other choice of name were not extravagant enough and so they both opted to go with this.
Road - Road
Road’s true name is a secret that most people are unaware of, she goes by this name since the Earl had said that they merry band of Akuma would pave the road to a new and better world.
Tyki - Joyd
Like the other members of the Noah, Tyki’s name was given to him by the Earl. As they all have names that relate back to ancient times when the miraculouses first came into being.
#dgm x ml au#shady au#dgm x ml#aaaaaaaaaand#like a century later i gained inspiration back for this au#i changed some of the heroes from what they originally were#but boom#i kind of think imma stick with these#unless someone comes up with something better#than i might opt out#but i think these are staying#so have fun with these yall
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Soulless Butterfly
Since I posted it on FF.net, I thought that I might as well post it here too.
Summary:
"What!?" It was just an accident. What started off as a simple search for lost companions in the Ark, turned into a fall that will give our heroes a trip of a lifetime. Watch as our 3 main exorcist and their scientist companion travel through a world completely unlike their own. "You call that an Akuma?!" Or was it…? Rated T for Kanda's French and some future themes.
Before you ask, this takes place before Timothy's arc. So the gang haven't been to Paris yet.
Disclaimer: I don't own DGM nor MLB.
So without further ado~ It's showtime!
Chapter 1: The Great Fall
A white haired teen was walking in a large dark hallway. It looked like something out of a horror film. Dark, brick walls all around him. With torches lighting the walls as he walked along. There was a noise in the distance, it sounded like…whining? As the teen walked, the sound grew louder and louder, until he was in front of a large door. Gentle pushing on it, the door swung open and a golden light flowed out of the doorway. He took a step and his foot connected with a sheet of paper. And another and another.
"But BRIGITTE! I need those papers!" there was a [semi] masculine whine.
There was a women with curly, short hair that was holding a stack of papers in her hand. She had a blank expression on her face. In front of her, looking up at her from the desk, was a man with dark purplish hair that curled at the ends. He had a white uniform like outfit with a rose cross on the chest.
"You do not, Mr. Lee. These are just plans for another Komlin." She spoke, her tone slightly monotone.
"Which I need! Nooo!" The man whined once more as the plans were taken from him. The white haired teen just smiled at the elder in sympathy. Noticing his presence, the man spoke. "Ah! Allen! You're here! Come in." the chief beckoned him.
Allen stepped further into the messy room. The white haired teen strolled over to the Chief. He nodded his head and smiled to the women, Brigitte, before focusing his attention on the man in front of him.
"The others will arrive shortly."
"What's going on, Komui?" Allen asked, confused.
"Well..We...Let's wait until the others get here, then I'll tell all of you at once. I do not want to repeat myself." Komui explained.
Suddenly the door swung open and in stepped two tall men. One had had fire red hair that spiked in all directions, but held back by a black bandana with a green X embroidered into it and on his face a black eyepatch. The other had his midnight blue hair tied back with a woven red tie. They were dressed in casual clothing.
The redhead had a dark green, long sleeved shirt. With the sleeves ending at the a little bit past the elbows. He wore white pants with a black belt and large boots that covered the lower part of his pants, past his knee. At his waist was a small black war-hammer. The other had a sleeveless dark blue shirt on with black pants. It exposed part of a black tattoo that seemed to crawl on his shoulders and arms. Around his waist was a white cloth* that had a scale looking texture to it and held a long sword. On his feet were black boots.
"What's the Moyashi doing here?" the midnight haired one grumbled.
Allen glared at the swordsman, "It's Allen, Bakanda." he growled.
Bakan- The swordsman growled back and grabbed the hilt of his sword, taking a step forward. The white haired teen responded by pulling on his white glove.
"Now, now! Yuu! Moyashi! No need to fight in Komui's office! It's already messy as it is!" The redhead laughed.
The two angered males turned to the laughing redhead. Their glares sharped. Making them look like predators after a small rabbit.
"I thought I told you. DO. NOT. CALL. ME. THAT!" the two synced before glaring at each other.
"You guys!" the chief called to them, but was ignored.
"What? They're just names! Right Yuu-chan~!"
"Usagi," The swordsman took a step to the redhead, "You have 3 seconds."
"Guys!"
"Ahaha! 3? Aww! Don't hurt me Yuu-chan!"
"1." he took another step.
The redhead took a step back and chuckled nervously. "Haha..Moyashi! You'll protect me right?"
Allen crossed his arms and glared at the redhead, "Don't call me that, Lavi. It's Allen!"
"2."
"GuYs!"
"OK, ok! Allen won't you protect me!?" Lavi cried out.
"No." Allen looked away and Lavi dramatically gasped.
"B-B-but Moyashi!" he whined.
"3!" the swordsman unsheathed his sword and ran at the Usagi.
"EXORCISTS!" the leader yelled halting all action.
They trio focused their eyes on him as he fixed his glasses.
"Now that I have your attention. I would like to inform you of the task."
They just nodded-well two of them did, the other just Tsked. They sat on the sofa and glared at each other-well Allen and Bakanda did-before looking away. Lavi just chuckled.
"Ok, so...We, the science team, want to examine and inspect the Ark more closely." Komui started.
"What does that have to do with us?" Bakanda asked.
"I'm getting to that. And so, we sent some of our scientists in and well...They got lost."
"They got lost?!" the trio looked at him is disbelief.
"Yes! So, we sent more in...and-"
"Let me guess...they got lost?" Lavi laughed but stopped at the look on the man's face.
"They...did."
"So you want us...to go in and find them?" Allen questioned in disbelief. They know I get lost just as easily! I can barely make it to the musician's room some days!
"Exactly!" Komui visibly brightened up.
The swordsman stood up. "That is not a job for an exorcist, Komui." he glared at the leader.
"I know! But it's the only small job I can give you! I promise to make it up to you, Kanda, after this! But we really need you guys on this!...No one else wanted to go in and look for them." Komui cried. "Well...actually...except for one."
"Then let them do it!" Kanda growled.
"Bakanda, maybe we should help. I mean, the Ark is a very big place, it's easy to get lost. Four people looking would be better than one. I don't really mind helping. I do need to learn the Ark's layout." Allen reasoned with Lavi nodding in agreement.
"Besides! I'm sure Panda-Jiji would want me to document more on it. So I say, I'm in!"
Kanda scoffed and turned to the door, ready to walk out. He ignored Komui's cries and just kept briskly walking to the door. Until he was stopped.
"Ok! Bakanda! If you do this with us, then I'll fight you when we get back." Allen tried to bribe him.
There was a moment of silence. The three anticipated the swordsman answer. Each hoping he would accept.
"Fine. You're on."
The three broke out in cheers.
Standing in front of massive white gate, Allen was decked out in his usual uniform. Black and red along with gold. On his chest was a badge of the famous Ross Cross. He looked to his side and found his fellow exorcists. They wore the same uniform as him. While his was just a jacket and pants, they had their very own versions of it. Kanda's had a long trench cloak like jacket instead of the short one. Lavi traded the pants for his normal white ones, which transitioned to black towards the ends. All of them sported a type of long black combat boots.
Suddenly, he felt something round pop from his uniform pocket. Looking down he saw a familiar golden sphere. With a face of a cross, tiny horns, and wings. Allen smiled before patting the being. It grinned, revealing a row of sharp teeth. A sight that terrified others but made the exorcist's smile grow warm with fondness.
"Well, what are we waiting for?! Let's go in!" Lavi chimed.
Of all the days Link is gone...
Without turning back the three climbed up the stairs and into the light. Upon entering the Ark, Allen heard the shouts of a female. Considering how quiet the Ark is, it wasn't so hard to hear them.
"Rikei! Shifu! Where are you?!" the female yelled out.
Wait...is that?
"It sounds like they're coming from that way." Kanda bluntly pointed out. "Lets go."
He walked off. I guess he really wants to get this over with? Lavi and Allen shared a look before following after him. As they drew closer, the shouts got louder. Soon they found themselves behind a very familiar scientist.
"Hey! It's Allen's-Ack!" Lavi received a swift elbow in the ribs before he finished that sentence.
The female turned to them. Her brown hair was in long, twin pigtails with flower hair ties. She had small, round framed glasses over their big brown eyes. She wore a long white science coat over a blue vest, covering a long sleeved white button up shirt, and a dark blue skirt.* Once she had saw them, she brightened up considerably and her cheeks were colored pink.
"Mr. Walker!" The scientist waved her hand to them and Allen thought he saw hearts in her eyes when she greeted him.
"Ms. Lou Fa, It's great to see you again." He greeted her with a smile.
"Did you get my present?" she asked cheerfully.
The white haired exorcist nodded his head, "Yes, it was good! Thank you for that."
"You're welcome!" She seemed to glow brighter.
"So little lady," Lavi draped his arm over the small women's shoulders, gaining her attention. "We heard you calling out to your buddies. Are they lost as well?" his eyebrows wiggled at the girl.
Lou Fa nodded her head. "Yeah, they went in to find the others who were lost and now I can't find them." she looked down, sadness in her eyes at the fact her friends are probably lost.
"Don't worry, we're here to help look for them." Allen reassured her, not wanting her to feel saddened.
The scientist's smile returned and his widened. Lavi looked at them and smirked, confusing the cursed exorcist. What is he smirking for?
"Yeah, little miss. We got this! I bet Moyashi, Yu-chan and I can easily find them." Lavi grinned at them.
I'm not! Ugh! "It's Allen, Lavi." The name irritated Allen to no end. First it was Kanda, then Lavi. He did not need Lou Fa calling him that too.
Kanda responded by unsheathing his sword. Lavi ducked behind Lou Fa.
"Ah! Don't hurt me Yuu!"
The swordsman growled. Allen noticed how Lou Fa seemed to be scared. Her eyes wide and staring at the swordsman as Lavi hid behind her. Sending a glare at the crazy bunny before turning his attention to the angered exorcist.
"Kanda, calm down. You can take your anger out on Lavi later." Allen glared at the swordsman, ignoring Lavi's cry of disbelief when he mentioned that Kanda can pretty much beat him up later.
"Don't tell me what to do, Moyashi." he glared back at him.
"It's Allen! Bakanda! Or can you not hear?" Allen smiled evilly at him.
Kanda aimed his sword at the parasite innocence user, while Allen tugged on his glove once again. There was a sudden fire around them partnered with bolts of electricity flying. Their glares and battle stances stayed. Before they went any further, they were stopped by a cute "Ah-hem." Allen looked over and saw a blushing Lou Fa, with a cowering Lavi behind her-still.
"Well, since you guys are h-here, I suggest we start looking! I want to help!"
Kanda tsked and sheathed his sword, looking away. Allen sent him another glare before fixing his glove and straightening up. He directed a smile at the scientist.
"You're right, but are you sure you want to come with? You probably have a lot of other work to do."
She shook her head, her blush intensified for some reason. "I'm sure, my friends are more precious to me. I-If they are lost then it's my duty as a friend to find them."
Lavi patted the girls head and came out from behind her. "Now aren't you just the sweetest?"
The scientist blushed shyly at the attention. Allen smiled at her once again, proud and happy with her answer.
"So! Which way do we go?" Lavi asked, crossing his arms behind his head in a casual pose.
Kanda grunted and headed towards the left, totally leaving them behind, much to Allen's irritation. Lavi jumped up and followed the swordsman with a "here we go." Allen sighed and looked at the female.
"Shall we get going?"
Her face became redder and she mustered a small nod, following after the cursed exorcist. The quartet walked on with Kanda leading them. Lou Fa occasionally shouted out to her lost companions, earning no reply each time. Eventually they wondered into an unfamiliar part of the Ark. In this area, doors were colored differently. Almost like the time when they fought the Noahs inside the Ark, not too long ago.
They walked past a blue door with a sword carved in it. A ebony door with...is that an H? And various others. It was when they passed a normal brown door, did Lou Fa stopped walking.
"Do you guys hear that?" She asked.
Allen shook his head, confused. The other two repeated the action.
"I don't hear anything." Kanda grumbled.
"Listen." She put her finger to her lips, the universal sign of 'Shh' or be quiet.
Allen shrugged his shoulders and closed his eyes to listen. At first he heard nothing, then he heard something...fluttering? Or was it flapping. He tried to pin-point the sound. It seems to be coming from-
"There, it sounds like it's from there." Lou Fa interrupted his train of thought as she pointed towards the brown door.
Now with a smaller yet closer examination, Allen saw in the door a cat chasing a ladybug. There were also butterflies in the corners of the door. The odd thing was, this door had no handle. In where a handle would be, was just a flat, gold piece of metal. Where is the handle?
Allen watched as she walked towards it and pressed her ear against it. The scientists looked her their way and beckoned them. He and Lavi exchanged at look before walking towards the door. Now that he was closer, Allen could most definitely gear the fluttering. It sounded like a mass hoard of...butterflies. Could they be a certain Noah's Teezes?
Suddenly to his horror, the door flew open. It seems the woman pressed to much against the door. Causing it to open. He saw the fear paint on her face before she fell in. Reacting on instinct, the cursed exorcist sprung forward into the opened door way after the fallen girl. Thankfully he gripped her hand in time. Until he realized he jumped in as well and didn't grab anything to keep him from falling. In desperation, he reached out and took hold of a boot? It was Lavi's, he recognized. His eyes wandered up and saw the redhead being held up by his shirt by Kanda, who was gripping onto the door frame with his body leaned in.
How ironic to be in this situation again.
"What the hell is the big idea?!." Kanda grumbled.
"Ack! Ca-Calm down! I-I'm sure we can get out of this...like last time!" Lavi choked out.
Lou Fa couldn't help looking down into the darkness. It seemed endless and so suffocating. It was like it could swallow them whole any second now. She did not expect the door to swing open like that. It had no handle, surely it would've stayed close. Too bad that wasn't the case. Oh how horrible she felt. If only she hadn't leaned on the door! She would be fine now!
And I dragged Mr. Walker down with me too! He must hate me now!
She felt tears pool up in her eyes. She felt so unlucky. A burden. Useless. All the negative feelings whirled inside her and she almost didn't notice the flapping of a butterfly. Her tear shot eyes shot up and at the flying creature. It was all black yet seemed to give off a purple glow. And it was heading right her direction.
Wait are those the things in the reports about that one Noah?!
Panic filled her. She had read about the cruel butterflies that killed one of the exorcist and injured Allen Walker to near death. And now the same beast was coming her way?!
She tightened her grip on Allen's hand. A tiny yelp left her throat as she panicked. She let her body sway around, trying to stay away from the flapping monster. Quickly, she let her eyes go up to the cursed exorcist, trying to convey her fear to him.
He looked down at her and she saw his eyes widen. He must've seen the horrid bug. She felt his grip tighten before he jerked his arm up. Lou Fa felt herself fly up, her hand momentarily leaving Allen's. Making her panic, before she felt an arm quickly snake around her waist and pull her toward a warm, solid chest.
She looked up and saw Allen's face scrunched into a glare. He was looking past her. Suddenly, her cheeks felt warm and she let out a small squeak. She was in his arms! She was being held in his ARMS! For a moment, the poor scientist forgot to breathe. She could smell the hints of vanilla and sweet dangos radiating off of his uniform. It took every fiber in her body to keep her from actively sniffing the guy. I don't want to come off as weird!
Suddenly, they were swinging and twisting. Lou Fa noticed the butterfly still flying after them. With each twist, it flew around only to fly back when they twisted the opposite way. This kept going on and to her it was like they were dancing in air with the ability to fall to their death anytime. Ugh, how romantic...
"ACK! Moyashi stop moving!" Lavi choked.
"I can't help it! There's a Teez down here! It's after Lou Fa for some reason!"
"A what?!" Kanda yelled, sounding strained.
Lou Fa and Allen kept swinging and twisting. Which was not helping Lavi at all. The boot Allen held onto, started to move and twist as well.
"S-Stop! My boot!" Lavi cried out in gasps, his shirt choking him.
Unable to take the shirt's choking hold, Lavi moved his head around trying to get comfortable and free.
"Usagi! Stop!" Kanda yelled in irritation.
Lavi coughed and suddenly his head slipped out of the hole. The relief was instant, but the realization of the action hit him hard. In desperation, he reached out and grabbed Kanda's wrist, tugging on it. Making the swordsman's grip slip. Lou Fa let out a scream and they all suddenly went tumbling down. Allen let go of Lavi's boot in favor of holding Lou Fa so that she wouldn't get hurt by whatever impact they were facing. Lavi was flying down shirtless. Kanda was falling with a scowl in his face, his grip on the shirt tightened as they all fell.
"Wayzz?" a gentle voice murmured
The owner of that voice was a short Chinese man. He was wearing a red Hawaiian shirt with beige pants. He was kneeling on a large futon-like mat and staring out of glass balcony doors at the sky. It was clear and sunny yet he knew better. In his hands was a cup of piping hot tea. It's aroma waved in the air and gave the room a tranquil feel to it. Floating next to him was a still creature. It resembled a turtle. With a shell and everything. It's eyes were wide and focused on the sky. It seemed to be frozen in shock.
"They're here." it whispered.
"Who's here? Wayzz?"
Wayzz's big eyes glazed over and plump tears rolled down his tiny face. A smile spread on his face. It let out a fond sigh and whispered.
"God's Children."
It took a long while before their bodies came in contact with the ground. But when they did, Allen and Lou Fa hit first. The cursed exorcist's back crashed against a flat, stone surface and his spine shook at the force of the impact. He winced at the pain. In his arms was the frightened scientist woman, who was clinging to him and trembling. Allen cracked an eye open. He was assaulted by the sight of his companions falling towards him. Thinking on the fly, he rolled to the side and let them crash next to him. Dust flew up into the air and obscured his vision, momentarily.
He heard a chorus of coughs before a familiar growl. "Fucking hell."
A choking gurgle spoke up next, "Ack! Y-Yuu. You're...crushing...me!"
There was a 'tsk' before a ruffle of movement and a very dramatic gasp. Allen carefully sat himself and Lou Fa up. The dust around them settled and he saw a Lavi, who was gasping for breath, and a very angry Kanda. The swordsman glared their way and grumbled.
"What the hell?!" the look he was giving him was very accusatory.
"Don't look at me like that, Bakanda!" Allen shot him a glare, unintendedly pulling the scientist closer in his irritation. Not noticing that her cheeks were flaring up.
The two bickered bitterly back and forth. The bookman apprentice snatched his shirt that had fallen to the ground. He shook it out, letting the dust fall off it before sliding it back on. He twisted his head from the bickering children and focused his attention the surroundings. He walked forward and grabbed onto a stone railing and took in a large gulp of air. The boy stopped and stared at the sight that was before his eyes.
"Guys."
"It's your fault we landed in here!" Kanda growled.
"It's no one's fault Bakanda! It was an accident!" Allen hissed.
"Allen. Kanda."
"I'll make your death look like an accident, Moyashi!" There was the metal sound of a sword being unsheathed.
"Just go ahead and try!"
"Allen! Kanda!" Lavi shouted and they halted.
"Tsk, what?"
The red head looked back at them and his single eye gleamed with a confusion and disbelief. "You have to see this."
Okay that was Soulless Butterfly. I hope you liked the first chapter so far! The first chapter is never my best, but just stick with it. I promise that it'll get better.
*1: White Cloth - seriously...what is that?
*2: Description of Lou Fa(Rohfa) - I choose the newer look she has for this story along with a colored picture of her with a blue vest and skirt.
So shippings...I know how crazy both of these fandoms are with those...so just so you know. I already have them planned out. Alright? Alright!
Ciao-su~
#Soulless Butterfly#dgm#dgm crossover#ml#ml crossover#allen walker#Lou Fa#lavi#kanda yu#Marinette Dupain-Cheng#adrien agreste#this has been the thing that's been plaguing my mind for the past few months#dgm x ml#miraculous ladybug#mlb#My writingz#Lovely's Fanfiction
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Information die kaum jemand weiß - Kochbuch Combi Dampfgarer ++ Mehr erfahren
Diese können im mitgelieferten Vorratsbecher, der 120 ml fasst, direkt nach der Zubereitung eingefüllt und im Kühl- oder Gefrierschrank aufbewahrt werden. Deine Checkliste ist in vielen Situationen enorm nützlich! Neben der Dampfstoßfunktion gibt es nun auch eine Vereinigung von Backofen und Dampfgarer in nur einem Gerät. Latest affordable siemens einbau dampfgarer hbd with dampfgarer einbaugert with einbau dampfgarer. Die meisten Elektrodampfer verfügen über drei übereinander angeordnete Container mit Löchern im Boden, https://drive.google.com durch die der Dampf hindurchfließen kann.
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Miele Combi Dampfgarer
Normale und elektronische Dampfgarer werden von den Unternehmen Tefal, Philips und Sharp verkauft. Entdecke die besten Dampfgarer des Jahres 2018 in.. Meist kann hier auch die Dauert des Garvorgangs mittels einer Zeitschaltuhr geregelt werden. Da beim drucklosen Dampfgaren, der erzeugte Dampf nach außen entweicht, wird im Inneren des Dampfgarers kein Druck aufgebaut und das Garen findet bei Umgebungsdruck statt.
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DGM X ML AU - KWAMIS
what? you didn’t think I’d leave the kwamis out did you? while these kwamis won’t be our beloved tikki and plagg, they’ll be a mixture from some dgm and mostly my own creativity. so if you guys have any better names for these kwami (which you probably will) send them over to me and talk to me about it!!!
as usual tagging @faeriexqueen @yugirl-with-dragons @kitty-ray since somehow they keep enabling me with this au
Black Cat Kwami: Timcampy
This one is probably the most obvious one.
While Tim appears as a golden cat like kwami, the rest of Allen’s super hero outfit doesn’t share these colors. The superhero outfit sticks to the black and white color scheme and Tim takes place as the golden bell that hangs around Allen’s neck.
Tim is a playful kwami that tends to get sidetracked alot. They also tend to be super forgetful and they like to poke fun at Allen for some silly things.
Ladybug Kwami: Nelli
If you figure out the nerd ref, props to you.
Nelli is the calm, positive kwami that Miranda is forever grateful that she has. Nelli’s calm nature helps Miranda with her panic attacks and her positive attitude helps Miranda build up her self-esteem.
These two together really balance out in terms of personality which they are both happy about.
Peacock Kwami: Feather (Boots as a nickname granted by Lenalee)
Boots real name is feather, but Lenalee gave them the nickname of boots since Feather has a tendency to nap in her hero’s boots.
Feather is full of energy and ready to jump headfirst into any situation. While the enthusiasm is great, this has led to some troubles in school with Lenalee trying to keep her super hero identity a secret.
Dragon Kwami: Pyros
Enter our teenage rebel kwami.
Pyros is a mischievous kwami that tends to cause Lavi more headaches than joy with their rebellious defiant nature. While Pyros and Lavi tend to butt heads a lot, when it comes to pranks, the two of them are partners in crime.
Bat Kwami: Wallachia
The most extra kwami you’ll ever meet in your entire life.
Seriously, like Wally had a mini fashion show for themselves when Krory had (as a joke) made them some costumes.
Wally loves the fact that their current hero is involved with the theater since they enjoy being the center of attention and must have the spotlight on them almost at all times. The only time he doesn’t is when he’s napping or simply not camera ready.
Monkey Kwami: Simian
Simian is the most curious out of all the kwami. They tend to go a little more dangerously out in the open since they want to explore the world.
Simian also tends to ask lots of questions.
About everything
They are also the most obidient of the kwamis.
Turtle Kwami: Helvlaska
Helvlaska is one of the older respected kwamis. They were the first Kwami to exist
They tend to be extremely knowledgeable in all verses of history including the creation of the miraculouses and their respective kwami.
While they have this knowledge, they tend to keep some parts of history a secret. Sharing too much information can cause problems for our heroes.
They are also able to sense when a potential wielder of the miraculous is nearby and therefore also chooses the wielder best suited for the miraculous.
They are also the most wise out of all the kwami and more often than not the other kwami will seek Helvlaska out for guidance when it comes to their heroes.
Moth Kwami: Lero
The last kwami to be created.
Once a kind bubbly kwami is now corrupted by the evil within the Earl’s heart.
Lero now has more cautious but vicious nature to their personality.
They mock their fellow kwamis for being weak and they seem to have a disdain for mankind.
They no longer care to preserve life, only to destroy it.
Rat Kwami: Rogue
Rogue is the most talkative out of all the kwami and with their nature being corrupted, has delved into more of the blackmail and gossip kind of talk.
They are easily described as two faced since their growing darker nature appears under the surface of their usual happy exterior.
Rogue also enjoys playing mind games with whoever falls victim to their traps that set up for it.
Snake Kwami: Ophidia
Tends to be one of the lazier kwami.
They enjoy sleeping and would rather rest than do much else.
The corruption of their nature has made them disinterested in life and to simply wish to join Altacia and Vixen in death.
Tiger & Fox Kwamis: No longer exist
Formerly known as ‘Altaica & Vixen’ respectively.
Altaica was the most serious kwami and also the most protective. When alive the other kwami would jokingly call them “mother” as they were overprotective of their fellow kwami and their wielder.
Vixen was once a vibrant trickster kwami with relentless energy. They enjoyed everything about life and they were the most appreciative out of all the other kwami.
These kwami gave their lives in an attempt to save their previous wielders, but ultimately it failed as their wielders died regardless.
Their bodies and their respective accessories were combined together through a series of experimentation and dark magic under the command of a secret organization that also knows about the miraculous powers.
While it took many failed trials, the combined bodies of the Altacia and Vixen and the accessories eventually bonded to Kanda and Alma respectively.
While this makes Kanda and Alma more powerful than the rest of the miraculous holders, this has major downsides.
Kanda and Alma no longer remember their life prior to the experiments.
The two of them both experience flashbacks or memories to the lives of the previous wielders of the tiger and fox miraculous.
This has sort of started to take a toll on their mental health.
#shady au#dgm x ml#dgm x ml au#and now i get to talk about the kwamis#what up#probably gonna more to this one later#but i think that this is pretty solid for the time being#welp here it is#and don't worry#theres more of these posts to come before i actually start writing this thing#still debating on whether or not to make it chapter based or just a series of one shots#ugh decisisons
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DGM x Miraculous AU - Villains and Heroes
Yep, so I’m making the au that nobody asked for. This post is literally just about the heroes and villains in this au with the miraculouses and their abilities. Don’t worry, I’m making another post later about settings, what’s happening with our heroes and villains, and some vague backstories lol.
Tagging both @yugirl-with-dragons (since she didnt believe this was an actual thing lol) and @faeriexqueen (since she indulged me into this au and now she gets to suffer with me lol)
Miraculous Holders: Villains
The Millenium Earl: Moth Miraculous
With this power, the Earl is able to create Akuma and also grant indivduals great super powers and enchanced capabilities (aka the Noah Family).
The Earl obejctive is to obtain all the miraculous and to rule over the world with all his incredible new powers.
Tyki: Snake Miraculous
Tyki is the current miraculous holder of the snake miraculous. This miraculous appears as a dark green bracelet wrapped around his left wrist.
With this miraculous, Tyki is able to:
Summon out a whip which much like a snake can constrict against an enemy.
Using his special ability, Serpent Strike to inject venom into his enemies to incapcitate them.
Like the other miraculous holders, Tyki has enchanced human reflexes, strength, and durability.
Road Kamelot: Rat Miraculous
Road Kamelot is the current miraculous holder of the Rat miraculous . This miraculous appears as a key chain that she usually places with her house keys.
With the rat miraculous, Road is able to:
Her tail on her outfit has a mind of its own, therefore it usually acts as a defensive block from behind when she’s occupied from the front.
Using her special ability Rogue of the Rat: Road can change shape and is able to get in or out of places that are impossibly hard to break into or escape from.
Like the other miraculous holders, Road has enhanced human reflexes, strength, and durability.
Road has a preference for hand to hand combat
The Rest of The Noah:
Okay, so I’m aware of the Noah clan, but even with the combined total of the miraculouses, there wasn’t enough.
So the Earl has granted them extra powers to be close to the level of fighting the other miraculous holders.
However, his influence holds them in too deep and therefore they choose not to go into their human forms since when they leave their akumatized forms, a lot of pain goes with it.
Miraculous Holders: Heroes
Allen Walker: Cat Miraculous
Allen Walker is the current miraculous hero of the Black Cat miraculous. His miraculous accessory appears as a black stealth glove on his left hand.
With the cat miraculous, Allen is able to:
With the use of his mask, Allen is able to see the Akuma’s true identity.
When he activates his special ability Crown Clown, Allen’s left hand gain sharp claws that can cut through any material. (This leads various accidents around the town since Allen can get carried away)
Like the other miraculous holders, Allen has enhanced human reflexes, strength, and durability.
Miranda Lotto: Ladybug Miraculous
Miranda Lotto is the current miraculous hero of the Ladybug miraculous. Her miraculous accessory appears as a charmed bracelet with different colored ladybug charms, which correspond to each of her special abilities.
With the use of the ladybug miraculous, Miranda is able to:
Sense the emotions of the akuma to attempt to figure out what the cause of their distress is.
When she uses her Time Record ability, Miranda is able to do the following abilities:
Time Out: Creates a space in which time stops, Miranda uses it as a shield for her fellow miraculous heroes.
Reverse: Miranda can target a creature or object, rewind its current state, and freeze it.
Time Recovery: Miranda can create a zone in which everything in that zone can revert back to its original state.
All of Miranda’s abilities are only temporary in the use of battle, when the akuma is caught and retains innocence, a form of Time Recovery is used to return the battleground back to it’s original state.
Miranda cannot reverse time on those who have died.
Like the other miraculous holders, Miranda has enhanced human reflexes, strength, and durability
Lenalee Lee: Peacock Miraculous
Lenalee is the current miraculous hero of the peacock miraculous . Her miraculous accessory appears as a feathered peacock hair pin.
With the use of the peacock miraculous, Lenalee is able to:
Move through the skies at high speed and increased leg strength.
When she activates her ability, Zephra’s Gales, Lenalee is able to create various damage inducing windstorms depending on her level of control.
Like the other miraculous holders, Lenalee has enhanced human reflexes, strength, and durability
Lavi: Dragon Miraculous
Lavi is the current miraculous hero of the dragon miraculous. His miraculous accessory appears as a necklace with a seemingly decorative war hammer hanging from the chain.
With the use of the dragon miraculous, Lavi is able to:
Make the hammer grow freakishly huge or smaller in size.
When he activates his ability Elemental stamp, Lavi is able to pick an element from the natural world with the use of his hammer and wield that element.
Like the other miraculous holders, Lavi has enhanced human reflexes, strength, and durability
Yu Kanda: Tiger Miraculous
Kanda is the current miraculous hero of the tiger miraculous. His miraculous accessory appears as a tattoo on the left side of his chest. When activated, the tattoo shifts to a more aggressive face of a tiger.
With the use of the tiger miraculous, Kanda is able to:
Have enhanced strength and agility
When Kanda activates his special ability Tygra’s Fury, he is able to:
Use his sword to cut down a swarm of akuma with a single strike.
Is able to create a barrier that keeps the enemy from leaving.
Like the other miraculous holders, Kanda has enhanced human reflexes, strength, and durability
Arystar Krory III: The Bat Miraculous
Krory is the current miraculous hero of the bat miraculous. His miraculous accessory appears as a brooch.
With the use of the bat miraculous, Krory is able to:
Fly for short distances and enhanced awareness of surroundings.
When he activates his special ability Nocturnal Flight, Krory can do the following:
Slink away into the shadow undetected
Use echolocation to find his way around
Mark the akuma he attacks so that the rest of his fellow comrades can strike them down.
Like the other miraculous holders, Krory has enhanced human reflexes, strength, and durability
Cross Marian: Monkey Miraculous
Cross Marian is the current miraculous lazy ass motherfucker hero to posses the monkey miraculous. His miraculous appears appears as part of a mask that covers the right side of his face.
With the monkey miraculous he can do the following:
Gain incredible agility and strength.
Enhanced flexibility.
Special abilities for the monkey is currently unknown as Cross tends to finish the akuma before it comes to that.
Like the other miraculous holders, Cross has enhanced human reflexes, strength, and durability
Froi Tiedoll: Turtle Miraculous
Tiedoll is the current miraculous hero of the turtle miraculous. His miraculous appears as his glasses that he wears.
With the turtle miraculous he can do the following:
Gain incredible durability
Stronger armor from the hero outfit.
When Tiedoll activates his ability, Healing Word, he is able to heal all comrades of his choosing within a certain radius.
Like the other miraculous holders, Tiedoll has enhanced human reflexes, strength, and durability
Alma Karma: Fox Miraculous
Alma is the current miraculous hero of the fox miraculous. Their miraculous appears as a fox tattoo on their lower right hip.
With the Fox miraculous, Alma is capable of:
Enchanced agility, reflexes, and flexibility.
When they activate their special ability Kitsune’s curse, Alma is able to create illusions that mess with the enemy’s psyche.
This could result fatally if the power is not controlled correctly.
Like the other miraculous holders, Alma has enhanced human reflexes, strength, and durability
The Other Miraculouses are currently missing and are being fought over by the two sides.
#dgm x ml#shady shitpost#shady au#miraculous ladybug#dgrayman#crossover au#omg i spent way too much time on this#and i still plan on making more posts#i might die#someone send help#im drowning in his au
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DGM X ML AU - SUPER HERO DISGUISES
you know how a lot of the time people question how no one knows clark kent is superman because of a pair of sunglasses? okay this is like that except not obvious (especially with allen’s white hair). So I���ve decided to make yet another post about what changes our wielders go through while they in/out of superhero mode. @kitty-ray @faeriexqueen @yugirl-with-dragons
Casual!Allen vs Hero!Allen:
So while Allen has white hair when he’s gone into super hero mode, his hair is normally a light brown when he’s not in disguise.
His eye color changes slightly when he’s in/out of disguise.
When he’s not in superhero mode, Allen’s eyes are dark grey, almost like the color of clouds during a thunderstorm.
When Allen is using his miraculous, his eye color shifts to lighter gray mixed with blue.
Also the scar he obtained as a child when mana died is mostly hidden by his mask, so it doesn’t raise suspicion.
Casual!Miranda vs Hero!Miranda:
Miranda has dark brown hair which is normally held up in a bun. When she’s in her superhero persona, her hair is loose and a bit wild.
Miranda also has more confidence in herself when she is in her superhero persona than she is in real life.
CasualLenalee vs Hero!Lenalee
Lenalee has long black hair that flows down to her lower back. She typically wears her hair down out of her persona.
When in her hero persona, her hair turns dark green and her hair is put into high pigtails.
Lenalee’s eyes are dark brown and when her miraculous disguise is active, her eyes turn purple.
Casual!Lavi vs Hero!Lavi
Lavi has a wild mess of red hair that is held out of his face with a headband.
When in suoerhero mode, the majority of his mask covers his face so that no one else picks up on the fact that its Lavi.
His voice also sounds rougher and deeper than his usual voice, which helps since Lavi’s voice is pretty distinct.
Casual!Kanda vs Hero!Kanda
Kanda is usually found wearing a regular pair of jeans with a hoodie (almost all the time) and usually has his black hair up in a ponytail to keep out of his face (why he doesnt just cut it, the world may never know).
When he’s transformed into his superhero persona, his hair color tends to change from varying shades of dark blue to a light purple (it depends on how angry he is and how much power of his miraculous he’s using).
While Kanda’s eyes are typically a dark blue color, when in the persona his eyes are a light blue.
Also, the pattern of his tattoo/location of his miraculous are visible on his superhero suit and the facial expression of the tiger changes depending on Kanda’s current constitution.
Casual!Alma vs Hero!Alma
Where do I even begin with the changes to this kid omfg
Alma has a tendency to dye his hair color differently every few weeks or so. Currently, his hair color is green at the bottom half of his hair which fades to a dark purple in the middle and eventually up to a light purple at the top. He’s very experiemtnal when it comes to hair color dyes and also cooking.
His Hair color under the magic disguise of the miraculous tends to be black.
Alma’s eye color tends to be light blue as himself
meanwhile, as a hero, his eyes are a dark green.
Casual!Krory vs Hero!Krory
Krory has one of the least changes to himself when it comes to transforming into his persona.
His black hair is usually slicked black prfessionaly for his job.
When he’s in his super hero persona, he gains a wide white streak through the middle of his hair and his hair gets more wild.
Krory also gains sharper teeth to go with the bat theme persona.
Casual!Road vs Villain!Road
Due to the nature of the miraculous being corrupted by evil, Road’s appearence changes are a little more drastic.
While as her human self, Road has pale white skin with dark blue eyes and matching blue hair. She also tends to dress more in a gothic style.
In her persona, Road has dark grey skin with yellow eyes. She also gains a form of black stigmata symbols across the top of her forehead.
Casual!Tyki vs Villain!Tyki
Tyki constantly changes between his clothing style between that of a high class rich person to a mismatched outfit that you’d find at the thrift store.
When in his persona, his appearence (like Road’s) changes drastically due to the miraculous being corrupted.
His fair complexion is changed to a dark brown.
He gains stigmata across his forehead
He also has yellow eyes, but the pupils tend to be more snake like.
Casual!Cross vs Hero!Cross
Cross is never usually in his hero persona since he doesn’t get into fights very often he usually just runs away if its not necessary so the changes made him when using his miraculous are currently unknown
His casual wear is usually a business formal outfit since he refuses to wear anything below that.
Casual!Tiedoll vs Hero!Tiedoll
Tiedoll is in the same situation as Cross. He hasn’t needed to use his miraculous for some time and therefore his hero persona has not been seen in some time.
Tiedoll’s causual wear usually consists of a mix of paint stained clothing and an old pair of jeans. Although, when it comes to public events, he dresses up for the occasion and doesn’t seem to mind the change in clothing.
The Millenium Earl is never seen out of his persona and the only thing to note is that his persona becomes creepier the more evil he becomes.
#dgm x ml au#shady au#will i ever stop with this au#lol no#i am in deep hell for this au#no seriously#like i have a notebook now#that how dedicated i am to it#.......#i have a problem#dgrayman#miraculous ladybug
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HAHAHAHAHHA
Guess who started writing the dgm x ml au that I created like forever and half ago... whoops
@faeriexqueen @yugirl-with-dragons @kitty-ray
#dgm x ml au#shady au#it's finally coming#I'm currently writing the first draft of the first chapter#oh boo#get ready for the memes#and angst#so much angst#and also the inherit randomness that is my sense of humor
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DGM x ML AU - The Differences
i told you that you guys can pry this au from my cold dead cursed hands. So here’s what I got so far on some of the varying differences in this au (ages, majors, jobs and living situations). @yugirl-with-dragons @faeriexqueen time for you two to join me in hell... and i guess @kitty-ray too even though she doesnt know dgm that well lol)
So ages, majors, and jobs for our characters are:
Kanda: 20
A third year in university and a double major in criminal justice and psychology
Kanda doesn’t really have a job since he does enjoy having some free time to himself, but he does get a spending allowance from Tiedoll (which he never uses unless its for Tiedoll’s birthday).
Kanda currently lives in a house with Tiedoll. He used to live on his own, but one day he showed back up on Tiedoll’s doorstep with all of his belongings and no warning. He moved back in with Tiedoll and he never asked Kanda why but he’s always wondered.
Allen: 18
A first year in university and a triple major in Accounting, Marketing, and Finance (someone save this poor boy)
Allen has a job at the university library and a job as a tutor for statistics. While it doesn’t pay much, Allen will gladly take job earned money rather than scamming people in a game of poker.
he only scams people for money if those people are like total douchebags
Allen currently lives on campus, he can’t afford to live off campus since that’s extra money he doesn’t want to spend. He has extra money stashed away just in case for emergencies since being with Cross has somewhat traumatized Allen when it comes to preserving money Although he has a room to himself since his roommate never exactly showed up and from what Allen hears, dropped out of university.
Lenalee: 18
A first year in university with a double major in journalism and communications
She has a job in the school newspaper, she freelances some work for the city newspaper as well and teams up with Alma for photos.
She also has another part time job at the local flower shop that she does during school breaks (typically during summer break).
Lenalee currently lives with her older brother Komui in an apartment that they share together.
Lavi: 21
A third year in university with a major in biochemistry and a minor in physics.
Lavi is a tutor in all of the sciences. He also has an internship at one of the research centers in London and is typically seen with Komui in the science department.
Lavi lives in an apartment on the other side of London which is awful when he has to get up early and make it to classes.
Alma: 21
A third year in university with a triple major in art, graphic design, and photography.
Alma has a freelance photography job that he gets paid for by the quality of photos. He also helps deliver pastries for his landlady’s business. Alma also has an unofficial internship with Tiedoll when it comes to art.
Alma currently lives in a loft above a bakery (think kiki’s delievery service style) which is pretty cozy.
Miranda, Krory, and Komui are all the same ages as they are in canon so they are: 26, 29, and 30 respectively.
Miranda is the clumsy history teacher that everyone enjoys having. Miranda also is a co-owner of a shop that displays old clocks and tell the tale of the clock’s jounrey before ending up at the shop. think of a museum for clocks but you can still buy it. Miranda lives in her apartment several blocks from the campus.
Krory is the over dramatic theater teacher assistant that is usually in charge of various productions put on by the theater department. Krory lives in one of the mansions located in London on the other side of town.
Komui is the overly passionate science department chair that gets way too carried away with personal experiments and laboratory tests.Lives in an apartment with Lenalee.
Cross Marian: 42 (although he’ll lie and tell the women that he’s somewhere in his early thirties)
He used to have a job as a lawyer, but no one knows why he suddenly quit one day and just disappeared. He travels around and no knows how in the world he still makes money, but he doesn’t seem to like London very much.
He adopted Allen when Allen was only ten years old.
Froi Tiedoll: 48 and damn proud of it
A retired art teacher from the Order University. He still restores art and sells art (art is a passion he will never let go), but he retired from teaching soon after he adopted Kanda (he already had Noise and Daisya under his care, Tiedoll is a tired dad) in order to spend more time with him.
He currently shares a house with Kanda and Daisya when he decides to pay a visit.
Road is 23
Graduated from university and majored in psychology.
Road lives with the other Noah in a mansion in the English countryside
Tyki is 25
Graduated from university and majored in business and minored in psychology
Tyki travels around through the U.K. (and formerly most of Europe before the battles started) and typically stays in cheap places until called back for battle. That’s when he returns to the mansion with the rest of the Noah clan.
Don’t ask the Earl for personal info, he might stab you
#dgrayman#dgm x ml au#shady au#this has been sitting in my drafts for too long#dear lord someone spazz with me on this au for the love of god#miraculous#i have no idea what to officially call this au#suggestions would be nice#cuz im drawing a blank and all of my creativity is going into these posts lol#no but seriously#someone help me#im dying
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TwinSpin and centrifugation: How it works?
TwinSpin centrifuge tubes support density gradient centrifugation. Let’s find out how and we will also discuss the protocol for the use of TwinSpin in combination with density gradient media.
Scientists divide materials using the centrifugation technique based on their size and shape. The two types of centrifugal methods used to separate particles are differential centrifugation and density gradient centrifugation. This blog will discuss TwinSpin's functionality as well as how it supports density gradient centrifugation.
Peripheral blood mononuclear cells (PBMC) can be separated from whole blood and bone marrow using TwinSpin centrifuge tubes that have already been pre-filled with PBMC Spin Medium. The TwinSpin is made up of an inner tube and a standard 15. The inner tube's open bottom is buried in the density gradient medium (DGM). The blood sampling tube can be directly pipetted into the TwinSpin with anticoagulated blood or bone marrow.
The sample is placed on top of the DGM inside the inner tube. Depending on the density gradient used (Leuko Spin, PBMC Spin, PBMC 24+, or PLT Spin Medium), leukocytes, lymphocytes, and PBMCs are separated from unwanted erythrocytes and granulocytes during Density Gradient Centrifugation.
Target cells above the DGM are therefore enriched in the interphase. Through the DGM, the erythrocytes will exit the inner tube and fall to the bottom of the outer tube. Once the inner tube has completely separated, just take it out.
As a valve, the elastic cap is used. The collection tube can be converted into a pipette by removing the cap. Drop by drop, the contents can be collected.
Instructions for Using the TwinSpin Tube
1. Ensure that the (diluted blood) sample, the TwinSpin, and the centrifuge are all at room temperature.
1.1 Check that the inner tube is partially filled and immersed in the DGM. If not, maintain the vertical position while rotating the TwinSpin device.
1.2 Take out and throw away the transport stopper
2. Include Sample Material
2.1 Pipette the sample material on top of the DGM in the inner tube by tilting the TwinSpin 45° and allowing the sample to run down the inside of the dropper.
2.2 Push the elastic cap firmly into the opening to close the TwinSpin. PluriSpin PLT Depletion can be used to reduce platelet contamination.
3. Spin
3.1 Centrifuge at 800 x g for 20 minutes at room temperature in a swing bucket rotor, brake off. If the blood is more than 4 hours old, centrifuge it for 30 minutes at 1000g.
4. Collect
4.1 Remove the inner separation tube by unscrewing it.
4.2 Push the elastic cup down to collect cells in the opaque layer in a new tube.
5 Wash (if necessary)
5.1 Add wash buffer to the reaction tube.
5.2 Spin down cells for 10 minutes at 4°C with 300 x g (no or small brake).
5.3 Remove the supernatant and set the reaction tube on the table for 20 seconds.
Excess wash buffer will flow down the tube wall and accumulate at the bottom. Aspirate the majority of the liquid above the pellet. The liquid will appear foggy because it contains mostly platelets; aspiration will improve the washing result.
5.5 Carefully pipette the pellet with 1 ml of wash buffer.
5.6 Re-do steps 5.1–5.4.
5.7 Refill the pellet to the desired volume.
Want to see TwinSpin in action? Check out our line of products that supports density gradient centrifugation, and let us know if you have any questions.
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₹ 79.00
#24
Sassoon® Original 2 Pcs N95 Protective Mask with Respirator, Can be Use as Pollution Mask & Face Mask, Best for Respiratory Protection, White Color)
3.3 out of 5 stars 3
₹ 330.00
#25
Salus Disposable Surgical Face Mask, Blue - 100 Pieces
3.1 out of 5 stars 1,031
₹ 228.00
#26
La Forte Safety Adult Face Shield, Fluid Resistant, Elastic Band and Soft Sponge- Sheet thickness- 500 Micron (.5 mm)(5)
3.3 out of 5 stars 26
₹ 318.00 - ₹ 1,199.00
#27
Omron NEC 101 Compressor Nebulizer For Child & Adult (White)
4.1 out of 5 stars 1,028
₹ 1,560.00 - ₹ 3,034.00
#28
Kodenipr Club Disposable Cap Stretchable Blue Bouffant Caps/Surgical Caps/Cooking Caps (100 Pieces)
3.9 out of 5 stars 87
₹ 115.00 - ₹ 625.00
#29
N95 Anti Pollution Mask With 5 Layer Filteration And Respirator - Re-usable Mask, DRDO & ISO-9001:2015 Approved with Filter.(White or Yellow)
4.6 out of 5 stars 3
₹ 180.00
#30
Sassoon® Original 5 Pcs N95 Protective Mask with Respirator, Can be Use as Pollution Mask & Face Mask, Best for Respiratory Protection (White Color)
4.0 out of 5 stars 2
₹ 699.00
#31
DOCTOR LE PARCO ® Medical Examination Disposable Gloves 20 PCs
2.8 out of 5 stars 10
₹ 299.00
#32
Venus Safety V-20 Cup Filter Respirator with Valve, ISI and DGMS Approved
3.7 out of 5 stars 11
#33
3M 6200 Medium Half Facepiece and 6003 Organic Vapour/Acid Gas Cartridge (Set of 2 Pcs)
4.4 out of 5 stars 25
₹ 3,045.00
#34
Urban Basics Mouth Nose Cover Respirator Anti Pollution Reversible, Reusable & Washable Cotton Unisex Face Mask (Multicolor, Pack of 10)
3.2 out of 5 stars 18
₹ 299.00
#35
SG HEALTH N95 Pollution Kids Dust Mask with Activated Carbon (Pink)
₹ 295.00
#36
Vritraz Kid Children N95 PM 2.5 Carbon Activated Anti Pollution Dust Face Mouth Mask with Replaceable Filter Green
₹ 499.00
#37
Lioncrown PN95 Reusable Anti Pollution Face Mask With Dual Respirator
2.5 out of 5 stars 63
₹ 399.00
#38
Nano Mist Sprayer
2.4 out of 5 stars 14
₹ 219.00
#39
Procare Disposable Latex Medical Examination Gloves (Medium) (100 Pcs)
3.0 out of 5 stars 16
₹ 495.00
#40
Ben Martin Industrial and Lab PPE KIT with Hoodcap (Color May Vary in White and Blue)
3.1 out of 5 stars 25
₹ 399.00
#41
Clasiko Face Shield Fluid Resistant Clear Full Face Masks Protective Anti-Splash Facial Cover with Elastic Band and Soft Sponge (5 Pcs)
2.8 out of 5 stars 12
₹ 277.00
#42
IndoSurgicals Silvery II-SS Stethoscope (Black)
4.2 out of 5 stars 1,383
₹ 1,299.00
#43
Portable Sanitizer Spray Bottle Pen 10 ML Empty (Set of 2) - Sanitizer Spray Pen Transparent, Refillable for Travel & Daily
3.5 out of 5 stars 3
₹ 135.00
#44
Airex N95 High Grade Mask for face, Filters Bacteria/n95 mask with respirator/Anti-Pollution Mask With n95 PM 2.5 Filter (White)|Pack of 3
2.7 out of 5 stars 3
#45
Lioncrown PN95 Luxuria Premium Pollution Respiratory Face Masks with UV Protection
4.5 out of 5 stars 2
₹ 399.00
#46
ZAPMASK Anti-Dust Anti Pollution Reusable Cotton Fabric Washable and Dust Proof Safety Face Mask
1.9 out of 5 stars 26
₹ 198.00
#47
Lsarimo Anti-Pollution N95 Face Mask With Carbon 2.5 Filter - NIOSH Approved (Grey, Pack Of 1)
2.5 out of 5 stars 3
₹ 299.00 - ₹ 799.00
#48
CaseX® N95 Anti Pollution Mask for Men And Women PM 2.5 Valve Face mask for Protection.
5.0 out of 5 stars 6
₹ 499.00
#49
HomeStrap 3 Layer Reusable 4 Piece Printed Fashion Safety Outdoor Reversible Face Mask/Cover (Multicolour)
3.6 out of 5 stars 46
₹ 369.00
#50
Honzen 10Pcs Transparent Protective Face Shield Anti-Fog Splash Oil-Splash Proof Full Face Protect Shield Anti-UV Anti-Shock with Clear Film Elastic Band and Comfort Sponge
2.4 out of 5 stars 51
₹ 260.00
https://ift.tt/303y5WJ
0 notes
Link
#1
Hoteon UREVO Mask-3ply Disposable Face Masks Pack with Built-IN Nose Pin, Bacterial Filtration Effiency(BFE) ≥ 95%,Particle Filtration Effiency (PEE) ≥93.6% , CE & ISO & SITRA Certified (Blue, Pack of 50,Sealed box packing)
3.8 out of 5 stars 64
₹ 599.00
#2
Careway Latex Medical Examination Disposable Hand Gloves, White, Medium, 100 Piece
2.9 out of 5 stars 1,674
₹ 480.00 - ₹ 730.00
#3
K-N95 Mask efficient protective K-N95 mask for adults, non-woven and melt blown fabric, particulate filtration, efficiency ≥95%, FFP2 for regular use, outdoor, travel, industrial activities
2.6 out of 5 stars 12
₹ 70.00 - ₹ 2,200.00
#4
Rudham Disposable Gloves, 300 Pieces, Transparent
3.3 out of 5 stars 1,895
₹ 195.00
#5
Kodenipr Club Disposable Stretchable Blue Bouffant Caps/Surgical Caps/Cooking Caps (100 Pieces)
3.4 out of 5 stars 435
₹ 148.00
#6
Bon Organik Reusable Cotton Gloves (Pack Of 10)
3.3 out of 5 stars 83
₹ 345.00
#7
Careway Latex Medical Examination Disposable Powderd Hand Gloves - 100 Pcs (Large)
2.7 out of 5 stars 484
₹ 570.00
#8
Billebon Nose Mask-3Ply Disposable Mouth Dust Pollution Mask 4 pcs
₹ 69.00 - ₹ 1,699.00
#9
URBAN HAAT Premium Disposable Latex Medical Examination Gloves 70 Pcs Medium Size
3.7 out of 5 stars 6
₹ 368.00 - ₹ 1,999.00
#10
Mirah Belle - Disposable Hand Gloves (White) - Pack of 10
5.0 out of 5 stars 8
₹ 100.00
#11
ARNV 3-Ply Disposable Surgical Mask with Advanced Melt-Blown Middle Layer, Set of 25
2.6 out of 5 stars 81
₹ 349.00 - ₹ 399.00
#12
Kodenipr Club Disposable Cap Stretchable White Caps - Head Cover Hair For Cooking & Hygiene(100 Pieces)
4.1 out of 5 stars 380
₹ 188.00
#13
Safe X Disposable Latex Medical Examination Gloves (100Pcs)| Powdered, Non Tearable, Made In Malaysia (Medium)
2.5 out of 5 stars 397
₹ 449.00
#14
Disposable Air Pollution & Protection Mask Face Mask Nose Mask Dust Mask surgical mask with tie (50)
2.2 out of 5 stars 718
₹ 125.80
#15
Chaithanya Orthopaedics Medicure Latex Gloves (White, Medium) - 100 Pieces
2.8 out of 5 stars 309
₹ 485.00
#16
Iconic personal protective equipment kit/PPE kit/set of 1
2.7 out of 5 stars 74
₹ 271.00
#17
Gilary Safety Face Shield, 5 Pcs Anti-fog Full Face Shield, Universal Face Protective Visor for Eye Head Protection, Anti-Spitting Splash Facial Cover for Women, Men (pack of 10 mask)(5 Pcs face sheild) And ( one hand senitizer)
3.3 out of 5 stars 34
₹ 429.00
#18
A & Y STORE KN-95 Face Mask Respirator Anti-Dust Breathable Protective Mask with N95 Filter for Adults men Women 5 layer protection Face Mask (Pack of 6)
3.9 out of 5 stars 21
₹ 155.00 - ₹ 499.00
#19
Go Hooked 350 Microns Safety Face Shield, Anti-fog Full Face Shield, (Pack of 10)
3.1 out of 5 stars 15
₹ 238.00 - ₹ 999.00
#20
AG E-Com Reusable Safety Face Shield, 10 Pcs Transparent Protective Shield, Anti-Saliva Windproof Dustproof Full Face Cover Hat for Men and Women (Combo) +Free 5pcs 3ply Mask
2.4 out of 5 stars 56
₹ 370.00
#21
Lioncrown Herring PN95 Reusable Outdoor Pollution Face Mask (Pack 1)
3.3 out of 5 stars 34
₹ 349.00
#22
Sassoon® Original 2 Pcs N95 Protective Mask with Respirator, Can be Use as Pollution Mask & Face Mask, Best for Respiratory Protection, White Color)
3.3 out of 5 stars 3
₹ 330.00
#23
Nitrile Surgical Black Gloves Medium (Pack of 100)
3.8 out of 5 stars 82
₹ 699.00
#24
N95/PM2.5 Anti pollution safety mask (Pack of 1)
2.6 out of 5 stars 11
₹ 81.00
#25
Salus Disposable Surgical Face Mask, Blue - 100 Pieces
3.1 out of 5 stars 1,031
₹ 228.00
#26
La Forte Safety Adult Face Shield, Fluid Resistant, Elastic Band and Soft Sponge- Sheet thickness- 500 Micron (.5 mm)(5)
3.3 out of 5 stars 26
₹ 318.00 - ₹ 1,199.00
#27
Omron NEC 101 Compressor Nebulizer For Child & Adult (White)
4.1 out of 5 stars 1,028
₹ 1,560.00 - ₹ 3,034.00
#28
N95 Anti Pollution Mask With 5 Layer Filteration And Respirator - Re-usable Mask, DRDO & ISO-9001:2015 Approved with Filter.(White or Yellow)
4.6 out of 5 stars 3
₹ 180.00
#29
Kodenipr Club Disposable Cap Stretchable Blue Bouffant Caps/Surgical Caps/Cooking Caps (100 Pieces)
3.9 out of 5 stars 87
₹ 115.00 - ₹ 625.00
#30
Sassoon® Original 5 Pcs N95 Protective Mask with Respirator, Can be Use as Pollution Mask & Face Mask, Best for Respiratory Protection (White Color)
4.0 out of 5 stars 2
₹ 699.00
#31
DOCTOR LE PARCO ® Medical Examination Disposable Gloves 20 PCs
2.8 out of 5 stars 10
₹ 299.00
#32
3M 6200 Medium Half Facepiece and 6003 Organic Vapour/Acid Gas Cartridge (Set of 2 Pcs)
4.4 out of 5 stars 25
₹ 3,045.00
#33
Venus Safety V-20 Cup Filter Respirator with Valve, ISI and DGMS Approved
3.7 out of 5 stars 11
#34
Urban Basics Mouth Nose Cover Respirator Anti Pollution Reversible, Reusable & Washable Cotton Unisex Face Mask (Multicolor, Pack of 10)
3.2 out of 5 stars 18
₹ 299.00
#35
SG HEALTH N95 Pollution Kids Dust Mask with Activated Carbon (Pink)
₹ 295.00
#36
Lioncrown PN95 Reusable Anti Pollution Face Mask With Dual Respirator
2.5 out of 5 stars 63
₹ 399.00
#37
Vritraz Kid Children N95 PM 2.5 Carbon Activated Anti Pollution Dust Face Mouth Mask with Replaceable Filter Green
₹ 499.00
#38
Procare Disposable Latex Medical Examination Gloves (Medium) (100 Pcs)
3.0 out of 5 stars 16
₹ 495.00
#39
Nano Mist Sprayer
2.4 out of 5 stars 14
₹ 219.00
#40
Ben Martin Industrial and Lab PPE KIT with Hoodcap (Color May Vary in White and Blue)
3.0 out of 5 stars 24
₹ 399.00
#41
Clasiko Face Shield Fluid Resistant Clear Full Face Masks Protective Anti-Splash Facial Cover with Elastic Band and Soft Sponge (5 Pcs)
2.8 out of 5 stars 12
₹ 277.00
#42
Portable Sanitizer Spray Bottle Pen 10 ML Empty (Set of 2) - Sanitizer Spray Pen Transparent, Refillable for Travel & Daily
3.5 out of 5 stars 3
₹ 135.00
#43
Lioncrown PN95 Luxuria Premium Pollution Respiratory Face Masks with UV Protection
4.5 out of 5 stars 2
₹ 399.00
#44
Lsarimo Anti-Pollution N95 Face Mask With Carbon 2.5 Filter - NIOSH Approved (Grey, Pack Of 1)
2.5 out of 5 stars 3
₹ 299.00 - ₹ 799.00
#45
Airex N95 High Grade Mask for face, Filters Bacteria/n95 mask with respirator/Anti-Pollution Mask With n95 PM 2.5 Filter (White)|Pack of 3
2.7 out of 5 stars 3
#46
CaseX® N95 Anti Pollution Mask for Men And Women PM 2.5 Valve Face mask for Protection.
5.0 out of 5 stars 6
₹ 499.00
#47
ZAPMASK Anti-Dust Anti Pollution Reusable Cotton Fabric Washable and Dust Proof Safety Face Mask
1.9 out of 5 stars 25
₹ 198.00
#48
IndoSurgicals Silvery II-SS Stethoscope (Black)
4.2 out of 5 stars 1,383
₹ 1,299.00
#49
Honzen 10Pcs Transparent Protective Face Shield Anti-Fog Splash Oil-Splash Proof Full Face Protect Shield Anti-UV Anti-Shock with Clear Film Elastic Band and Comfort Sponge
2.4 out of 5 stars 51
₹ 260.00
#50
Shakuntla Disposable Non-Woven Bouffant Surgical Head Cap (Blue) -50 Pieces
3.5 out of 5 stars 16
₹ 103.00 - ₹ 234.00
https://ift.tt/303y5WJ
0 notes
Link
#1
Hoteon UREVO Mask-3ply Disposable Face Masks Pack with Built-IN Nose Pin, Bacterial Filtration Effiency(BFE) ≥ 95%,Particle Filtration Effiency (PEE) ≥93.6% , CE & ISO & SITRA Certified (Blue, Pack of 50,Sealed box packing)
3.8 out of 5 stars 63
₹ 599.00
#2
Careway Latex Medical Examination Disposable Hand Gloves, White, Medium, 100 Piece
2.9 out of 5 stars 1,674
₹ 480.00 - ₹ 730.00
#3
K-N95 Mask efficient protective K-N95 mask for adults, non-woven and melt blown fabric, particulate filtration, efficiency ≥95%, FFP2 for regular use, outdoor, travel, industrial activities
2.6 out of 5 stars 12
₹ 70.00 - ₹ 2,200.00
#4
Rudham Disposable Gloves, 300 Pieces, Transparent
3.3 out of 5 stars 1,895
₹ 195.00
#5
Kodenipr Club Disposable Stretchable Blue Bouffant Caps/Surgical Caps/Cooking Caps (100 Pieces)
3.4 out of 5 stars 435
₹ 148.00
#6
Bon Organik Reusable Cotton Gloves (Pack Of 10)
3.3 out of 5 stars 83
₹ 345.00
#7
Careway Latex Medical Examination Disposable Powderd Hand Gloves - 100 Pcs (Large)
2.7 out of 5 stars 484
₹ 570.00
#8
Billebon Nose Mask-3Ply Disposable Mouth Dust Pollution Mask 4 pcs
₹ 69.00 - ₹ 1,699.00
#9
URBAN HAAT Premium Disposable Latex Medical Examination Gloves 70 Pcs Medium Size
3.7 out of 5 stars 6
₹ 368.00 - ₹ 1,999.00
#10
Mirah Belle - Disposable Hand Gloves (White) - Pack of 10
5.0 out of 5 stars 8
₹ 100.00
#11
ARNV 3-Ply Disposable Surgical Mask with Advanced Melt-Blown Middle Layer, Set of 25
2.6 out of 5 stars 81
₹ 349.00 - ₹ 399.00
#12
Kodenipr Club Disposable Cap Stretchable White Caps - Head Cover Hair For Cooking & Hygiene(100 Pieces)
4.1 out of 5 stars 380
₹ 188.00
#13
Safe X Disposable Latex Medical Examination Gloves (100Pcs)| Powdered, Non Tearable, Made In Malaysia (Medium)
2.5 out of 5 stars 397
₹ 449.00
#14
Disposable Air Pollution & Protection Mask Face Mask Nose Mask Dust Mask surgical mask with tie (50)
2.2 out of 5 stars 717
₹ 125.80
#15
Chaithanya Orthopaedics Medicure Latex Gloves (White, Medium) - 100 Pieces
2.8 out of 5 stars 309
₹ 485.00
#16
Iconic personal protective equipment kit/PPE kit/set of 1
2.8 out of 5 stars 73
₹ 271.00
#17
Gilary Safety Face Shield, 5 Pcs Anti-fog Full Face Shield, Universal Face Protective Visor for Eye Head Protection, Anti-Spitting Splash Facial Cover for Women, Men (pack of 10 mask)(5 Pcs face sheild) And ( one hand senitizer)
3.4 out of 5 stars 33
₹ 429.00
#18
A & Y STORE KN-95 Face Mask Respirator Anti-Dust Breathable Protective Mask with N95 Filter for Adults men Women 5 layer protection Face Mask (Pack of 6)
3.9 out of 5 stars 21
₹ 155.00 - ₹ 499.00
#19
Go Hooked 350 Microns Safety Face Shield, Anti-fog Full Face Shield, (Pack of 10)
3.1 out of 5 stars 15
₹ 238.00 - ₹ 999.00
#20
AG E-Com Reusable Safety Face Shield, 10 Pcs Transparent Protective Shield, Anti-Saliva Windproof Dustproof Full Face Cover Hat for Men and Women (Combo) +Free 5pcs 3ply Mask
2.4 out of 5 stars 56
₹ 370.00
#21
Lioncrown Herring PN95 Reusable Outdoor Pollution Face Mask (Pack 1)
3.3 out of 5 stars 34
₹ 349.00
#22
Sassoon® Original 2 Pcs N95 Protective Mask with Respirator, Can be Use as Pollution Mask & Face Mask, Best for Respiratory Protection, White Color)
3.3 out of 5 stars 3
₹ 330.00
#23
Nitrile Surgical Black Gloves Medium (Pack of 100)
3.8 out of 5 stars 82
₹ 699.00
#24
N95/PM2.5 Anti pollution safety mask (Pack of 1)
2.6 out of 5 stars 11
₹ 83.00
#25
Salus Disposable Surgical Face Mask, Blue - 100 Pieces
3.1 out of 5 stars 1,031
₹ 228.00
#26
La Forte Safety Adult Face Shield, Fluid Resistant, Elastic Band and Soft Sponge- Sheet thickness- 500 Micron (.5 mm)(5)
3.3 out of 5 stars 26
₹ 318.00 - ₹ 1,199.00
#27
Omron NEC 101 Compressor Nebulizer For Child & Adult (White)
4.1 out of 5 stars 1,028
₹ 1,560.00 - ₹ 3,034.00
#28
N95 Anti Pollution Mask With 5 Layer Filteration And Respirator - Re-usable Mask, DRDO & ISO-9001:2015 Approved with Filter.(White or Yellow)
4.6 out of 5 stars 3
₹ 180.00
#29
Kodenipr Club Disposable Cap Stretchable Blue Bouffant Caps/Surgical Caps/Cooking Caps (100 Pieces)
3.9 out of 5 stars 87
₹ 115.00 - ₹ 625.00
#30
Sassoon® Original 5 Pcs N95 Protective Mask with Respirator, Can be Use as Pollution Mask & Face Mask, Best for Respiratory Protection (White Color)
4.0 out of 5 stars 2
₹ 699.00
#31
DOCTOR LE PARCO ® Medical Examination Disposable Gloves 20 PCs
2.8 out of 5 stars 10
₹ 299.00
#32
3M 6200 Medium Half Facepiece and 6003 Organic Vapour/Acid Gas Cartridge (Set of 2 Pcs)
4.4 out of 5 stars 25
₹ 3,045.00
#33
Venus Safety V-20 Cup Filter Respirator with Valve, ISI and DGMS Approved
3.7 out of 5 stars 11
#34
Urban Basics Mouth Nose Cover Respirator Anti Pollution Reversible, Reusable & Washable Cotton Unisex Face Mask (Multicolor, Pack of 10)
3.2 out of 5 stars 17
₹ 299.00
#35
SG HEALTH N95 Pollution Kids Dust Mask with Activated Carbon (Pink)
₹ 295.00
#36
Lioncrown PN95 Reusable Anti Pollution Face Mask With Dual Respirator
2.5 out of 5 stars 63
₹ 399.00
#37
Vritraz Kid Children N95 PM 2.5 Carbon Activated Anti Pollution Dust Face Mouth Mask with Replaceable Filter Green
₹ 499.00
#38
Procare Disposable Latex Medical Examination Gloves (Medium) (100 Pcs)
3.0 out of 5 stars 16
₹ 495.00
#39
Nano Mist Sprayer
2.4 out of 5 stars 14
₹ 219.00
#40
Ben Martin Industrial and Lab PPE KIT with Hoodcap (Color May Vary in White and Blue)
3.0 out of 5 stars 24
₹ 399.00
#41
Clasiko Face Shield Fluid Resistant Clear Full Face Masks Protective Anti-Splash Facial Cover with Elastic Band and Soft Sponge (5 Pcs)
2.8 out of 5 stars 12
₹ 277.00
#42
Portable Sanitizer Spray Bottle Pen 10 ML Empty (Set of 2) - Sanitizer Spray Pen Transparent, Refillable for Travel & Daily
3.5 out of 5 stars 3
₹ 135.00
#43
Lioncrown PN95 Luxuria Premium Pollution Respiratory Face Masks with UV Protection
4.5 out of 5 stars 2
₹ 399.00
#44
Lsarimo Anti-Pollution N95 Face Mask With Carbon 2.5 Filter - NIOSH Approved (Grey, Pack Of 1)
1.0 out of 5 stars 2
₹ 299.00 - ₹ 799.00
#45
Airex N95 High Grade Mask for face, Filters Bacteria/n95 mask with respirator/Anti-Pollution Mask With n95 PM 2.5 Filter (White)|Pack of 3
2.7 out of 5 stars 3
#46
CaseX® N95 Anti Pollution Mask for Men And Women PM 2.5 Valve Face mask for Protection.
5.0 out of 5 stars 6
₹ 499.00
#47
ZAPMASK Anti-Dust Anti Pollution Reusable Cotton Fabric Washable and Dust Proof Safety Face Mask
1.9 out of 5 stars 25
₹ 198.00
#48
IndoSurgicals Silvery II-SS Stethoscope (Black)
4.2 out of 5 stars 1,383
₹ 1,299.00
#49
Honzen 10Pcs Transparent Protective Face Shield Anti-Fog Splash Oil-Splash Proof Full Face Protect Shield Anti-UV Anti-Shock with Clear Film Elastic Band and Comfort Sponge
2.4 out of 5 stars 51
₹ 260.00
#50
Shakuntla Disposable Non-Woven Bouffant Surgical Head Cap (Blue) -50 Pieces
3.5 out of 5 stars 16
₹ 103.00 - ₹ 234.00
https://ift.tt/303y5WJ
0 notes