#ministrainer

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Buy 1000 MiniStrainers, Get 1000 FACS Tubes FREE!

The pluriStrainer Mini is designed for precise sample filtration in laboratories. It fits various tube sizes, offers multiple pore sizes, and ensures sterile, hassle-free use, making it a lab essential for reliable results. 40 um cell strainer and different sizes are available in UberStrainer.

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TwinSpin and centrifugation: How it works?

TwinSpin centrifuge tubes support density gradient centrifugation. Let’s find out how and we will also discuss the protocol for the use of TwinSpin in combination with density gradient media.

Scientists divide materials using the centrifugation technique based on their size and shape. The two types of centrifugal methods used to separate particles are differential centrifugation and density gradient centrifugation. This blog will discuss TwinSpin's functionality as well as how it supports density gradient centrifugation.

Peripheral blood mononuclear cells (PBMC) can be separated from whole blood and bone marrow using TwinSpin centrifuge tubes that have already been pre-filled with PBMC Spin Medium. The TwinSpin is made up of an inner tube and a standard 15. The inner tube's open bottom is buried in the density gradient medium (DGM). The blood sampling tube can be directly pipetted into the TwinSpin with anticoagulated blood or bone marrow. 

The sample is placed on top of the DGM inside the inner tube. Depending on the density gradient used (Leuko Spin, PBMC Spin, PBMC 24+, or PLT Spin Medium), leukocytes, lymphocytes, and PBMCs are separated from unwanted erythrocytes and granulocytes during Density Gradient Centrifugation. 

Target cells above the DGM are therefore enriched in the interphase. Through the DGM, the erythrocytes will exit the inner tube and fall to the bottom of the outer tube. Once the inner tube has completely separated, just take it out. 

As a valve, the elastic cap is used. The collection tube can be converted into a pipette by removing the cap. Drop by drop, the contents can be collected.

Instructions for Using the TwinSpin Tube

1. Ensure that the (diluted blood) sample, the TwinSpin, and the centrifuge are all at room temperature.

1.1 Check that the inner tube is partially filled and immersed in the DGM. If not, maintain the vertical position while rotating the TwinSpin device.

1.2 Take out and throw away the transport stopper

2. Include Sample Material

2.1 Pipette the sample material on top of the DGM in the inner tube by tilting the TwinSpin 45° and allowing the sample to run down the inside of the dropper.

2.2 Push the elastic cap firmly into the opening to close the TwinSpin. PluriSpin PLT Depletion can be used to reduce platelet contamination.

3. Spin

3.1 Centrifuge at 800 x g for 20 minutes at room temperature in a swing bucket rotor, brake off. If the blood is more than 4 hours old, centrifuge it for 30 minutes at 1000g.

4. Collect

4.1 Remove the inner separation tube by unscrewing it.

4.2 Push the elastic cup down to collect cells in the opaque layer in a new tube.

5 Wash (if necessary)

5.1 Add wash buffer to the reaction tube.

5.2 Spin down cells for 10 minutes at 4°C with 300 x g (no or small brake).

5.3 Remove the supernatant and set the reaction tube on the table for 20 seconds.

Excess wash buffer will flow down the tube wall and accumulate at the bottom. Aspirate the majority of the liquid above the pellet. The liquid will appear foggy because it contains mostly platelets; aspiration will improve the washing result.

5.5 Carefully pipette the pellet with 1 ml of wash buffer.

5.6 Re-do steps 5.1–5.4.

5.7 Refill the pellet to the desired volume.

Want to see TwinSpin in action? Check out our line of products that supports density gradient centrifugation, and let us know if you have any questions.

[milena-bond]

My birthday is coming up, it's time to learn how to make cocktails. Just today I received a steel set of tools for their preparation from the La Kitchen Applesses: OXO GOOD GRIPS STEEL SPINNING BAR SPOON, OXO STEEL BOTTLE POURER STAINLESS STEEL Ensures preregistered portion control Helps mix cocktails quickly and efficiently. Prevents drips and soils. Fits most size liquor bottles. Controls the pour for more accurate measurements. OXO GOOD GRIPS GOOD GRIPS 3-INCH MINI STRAINER Whether you're separating seeds or mulling spices, the Mini Strainer is a useful tool to have on hand. It features fine mesh for thorough straining, and stainless steel, double-rod construction for strength and stability. The small loop at one end allows it to balance atop cups for one-handed use. Handy for brewing tea, straining, and separating, OXO STEEL COCKTAIL STRAINER, which the Strainer's raised lip forces liquids to strain through the springs, preventing drips and puddles. The soft finger rest offers comfort while straining, and the short handle allows for convenient storage. Designed to strain cocktails without dripping. Durable stainless steel body effectively strains ice, fruit, and more from drinks for smooth cocktails and OXO GOOD GRIPS STEEL ICE TONGS . All goods are Dishwasher Safe. For making Mojito I'm gonna use: Lime 1 pc 5-7 fresh mint twigs Sugar syrup (or 3 teaspoons of sugar) 20 ml Highly carbonated drinking water (or soda) Sprite Ice. Usually used crashed ice, but since there will be minors among the guests, I will prepare non-alcoholic Mojito and I will use figured ice for more beautiful look. If you decide to make Mohito after this article and use crushed ice, put it in a tight plastic bag, and wrap the bag in a towel. Then knock on it with a rolling pin or a hammer for chops until the ice turns into a fine crumb. Also I will need: OXO Spinning Bar Spoon 12IN. And OXO GOOD GRIPS STEEL ICE TONGS . Washing lime and cut in half. One half of lime cutting into small slices, the other half I'll use to decorate the finished cocktail. Next step: Washing mint and separating the leaves from the twigs. Putting into the cooled glass. Crushing with Spinning Bar Spoon until a bright mint flavor appears. The Spinning Bar Spoon helps ensure proper technique without the effort. It has the length of a classic bar spoon, but doesn’t require changing your grip to keep the back of the spoon against the edge of the glass. Spoon spins while I stir for effortlessly professional result Now adding lime wedges. To make sugar syrup, using pour 3 tsp of sugar into a small saucepan and pour 3 tsp of water. Bring to a boil and dissolve the sugar. Once the sugar has dissolved, the syrup is ready. Cool the prepared syrup quickly by placing the pan on ice or in cold water. I don't eat sugar, so I'm adding honey. Also Instead of sugar syrup, you can add sugar in a glass with mint and lime. In this case, it's better to add a little soda water to the sugar and stir until the sugar is completely dissolved. Sugar can be used white or brown. Brown sugar gives Mojito a subtle caramel flavor. If you making alcoholic mojito - just add rum to the cocktail. Now mixing gently with OXO Spinning Bar Spoon, trying to grab the ingredients from the bottom of the glass. CONTOURED HANDLE provides a comfortable grip. Spoon spins while you stir for effortlessly professional result. 12 IN LENGTH easily gets to the bottom of tall mixing glasses. Pouring some chilled sprite. And also knead until the juice appears. Now you can pour in rum, if you making alcohol Mojito. Adding ice into the glass to the top. With OXO GOOD GRIPS STEEL ICE TONGS, STAINLESS STEEL I'n not worry about slipping on errant ice cubes scattered on the floor, thanks to Ice Tongs’ sharp teeth! They make it easy to grab and hold ice and place it into glasses. Soft grips and a locking switch provide comfort and convenience. . Because they get a solid grip on ice when . serving cocktails. Chilling drinks requires gently spinning , especially if you making spirit-based ice in the glass to keep the cocktails crisp and clear, and to help prevent frothing or unwanted ice chips that could occur while shaking – OXO GOOD GRIPS STEEL SPINNING BAR SPOON, helps to stir like an expert that's very important, because the proper technique required to stirring and also prevent the ice from chipping. Pouring sparkling water, filling the glass to the top. Garnish Mojito with a slice of lime, a sprig of mint and serve immediately. It's 82 degrees today the ice is melting fast and the stars of ice are become rounded. Apparently, next time, I will use crushed ice for the preparation, add figured ones for decoration just before serving. Let me know in comments if you tried to make mohito, after reading this blog, did you used sugar / sugar syrop oh honey like me? which kind of ice did you used crashed or figured? Did you used OXO GOOD GRIPS STEEL SPINNING BAR SPOON, OXO GOOD GRIPS STEEL ICE TONGS ? If yes, how do you like them? You can find them on https://dealspotr.com/getcode/iF5KB (Or click the link La Cuisine Appliances in linktree in my bio) USE code MILENANARCISO Follow La Cuisine Appliances Promo Codes & Deals for the most updated list of deals.. Have a great weekend!

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