#autofluorescence

[aardwolfpack]

A fluorescent ant by Dr. Jan Michels.

[conspectusargosy]

Salvia mellifera, black sage, is a perennial shrub in the mint/sage family native from central California down through the top of Baja California. It is part of the cast of plants present in coastal sage and chaparral habitat, thriving only in a specific range of rainfall in a mediterranean climate. The flowers host a variety of pollinators including butterflies, hummingbirds, and native bees and its seeds, like other Salvia species, are abundant and provide food for animals and birds. The fragrant leaves contain chemicals used by the Chumash to treat pain and a unique spicy honey is derived from the plant in the modern day.

[biologist4ever]

Plant root growth under microscope

[bpod-bpod]

Trust Your Eyes

"Can’t see the wood for the trees". It isn’t just an old English proverb, it’s also a problem scientists grapple with when imaging whole organs during animal model experiments. They want to see the fine details (the trees) but also capture the entire organ (the wood). Researchers now present an affordable, rapid and high-resolution approach to image whole organs in 3D called TRUST (Translational Rapid Ultraviolet-excited Sectioning Tomography). Using an inexpensive ultraviolet LED light and a colour camera, TRUST imaging of sections through mouse tissue captured images of fluorescent dyes bound to specific molecules and autofluorescence generated by unstained tissue. The result? Detailed 3D images of whole mouse organs, such as the brain and heart, as well as tissue networks, such as blood vessels of the brain (pictured) and nerves. Entire mouse embryos were also imaged, revealing how TRUST can help investigate health and disease, as well as development.

Written by Lux Fatimathas

Video from work by Wentao Yu and colleagues

Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology, Hong Kong, Hong Kong, China

Video originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)

Published in eLife, November 2022

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[sitting-on-me-bum]

Autofluorescence of the tip of the stamens of flowers at 405 nm, 488 nm, and 561 nm, emitting a greenish blue and emerald green color like peacock feathers.

By Mei Yu (China)

Light Microscopy Awards

[thereissomethingunderthestairs]

Hey everyone go to bonecrate dot gov and use promo code Sighialmr for 20 Us dollars off your first monthly crate that ill send you (note some boxes may contain violations of your states protected species act and i am not to be held liable)

[reportwire]

Fresh understanding of ageing in the brain offers hope for treating neurological diseases

Newswise — Scientists from the Trinity Biomedical Sciences Institute (TBSI) have shed new light on ageing processes in the brain. By linking the increased presence of specialised immune cells to conditions such as Alzheimer’s disease and traumatic brain injury for the first time, they have unearthed a possible new target for therapies aimed at treating age-related neurological diseases. The…

[drafthearse]

Hazelnut (male flower), overlay of 7 channel autofluorescence microscopy. Imaged with ZEISS Axio Observer, Axiocam, Colibri 7.

[eudikot]

you're a researcher!! that's so cool! if you don't mind me asking, what do you do?

Thank you for the ask, I love talking about my research!! Currently, I'm working on studying barley and its interaction with Pseudomonas fluorescens using a clear hydrogel media instead of soil. I got interested in this project because I think that the ability to grow plants in hydrogel instead of soil to study their roots is such a cool thing, and since I'm majoring in both plant and microbial biotechnology adding the bacterial aspect helps bring everything I'm working on all together (and is something I can make a paper out of). Then to add biotechnology into the equation, we added GFP (green fluorescent protein) into the bacteria, which causes them to glow green when hit with UV light and viewed under a filter. This makes it easier to see the bacteria on the roots.

Because this projects excites me so much and has some cool pictures, I have to share some. The first picture are roots without any bacteria and the second one has the bacteria. Feel free to take a second and see if you can identify any differences between the two images.

The first noticeable thing I see is within the hydrogel itself. The non-bacterial control has a much darker background, likely because there are no bacteria to fluoresce in it. However, you may notice that the roots still have a glow to them. That's actually because plants have autofluorescence based on the proteins/chemicals they produce which was visible through the filter. Now, the things that stand out to me on the bacterial roots compared to the regular ones are that they almost have a rougher appearance, likely due to the bacteria colonies growing on it. And then there is the bright spots of bacteria that are not present on the bacteria-free roots. Seeing such a stark difference so easily (since I have been looking at images of these for a while now and know what I'm looking for) honestly blew me away when this actually seemed to work.

Also, just to talk about autofluorescence again, the chlorophyll in the leaves make the plants so bright in this system. I mean look at that leaf!! I never though I'd see such a pretty plant (the last one looks slightly different from the rest because it was taken with my phone instead of the microscope camera, but catches the entire autofluorescence really well).

And to wrap this whole spiel up, let me give a little background on where I started with doing research. I actually started out by doing data analysis on differential gene expression, or the differences in expression under different conditions. A lot of analysis uses an "out of 100%" model if you will, and because of this if one gene changes expression a lot but everything else stays the same, it'll look like all those genes have lower expression compared to the one that changed even if they might not. You can see how that might be a bit of a problem. I was testing a new R package that would compare gene expression in a different way to bypass this problem and more clearly see what is actually happening to different genes.

Because I was just helping a bit on this project I never saw its conclusions before moving onto other things. I took a side off of that project to get a grant and do a systematic review on how other researchers are describing their gene expression clustering, since in R you need to define the clustering method (how different groups are compared to one another) and the number of clusters you want. Because these are human entered and can change the outcome of the data you're analyzing, it's important that what you do can be replicated, but a lot of researchers were very vague in even what packages they used to cluster. I'm still finishing up that work so hopefully I'll be able to publish a paper on it soon.

Then I've also had fun helping some of our grad students with barley biomass measurements and, my absolute favorite, soil microbe DNA extraction. That student is using differences in the soil microbiome to see if it has an impact on the growth of the barley, and is actually what inspired me to student plant-microbe interactions!

[conspectusargosy]

Dudleya cymosa ssp. pumila is a succulent native and endemic to California, meaning it cannot be found growing naturally anywhere else in the world. This plant is most often found on north-facing canyon walls and roadcuts throughout the transverse ranges (a series of mountains that run east-west through CA instead of the usual north-south.) Dudleya in general have special adaptations which allow them to survive challenging environments, in this case growing in the wet but coldest part of the year and surviving a long, very hot, and very dry summer. Even in this subspecies there are many included forms, with varying traits such as flower color, leaf farina, altitude, etc., which goes in hand with Dudleya being a genus of complicated and still-developing taxonomy with quite a lot of undescribed species. Molecular phylogeny is making this possible by sampling genetic material to determine evolutionary relationships. This process is further complicated by the fact that Dudleya is a highly poached genus and many populations of unique plants are threatened by sprawling human development and introduced herbivores before they even have a chance to be recognized. Dudleya is one of my favorite genera of plants, so expect to see more of them in my posts in the future!

[health-views-updates]

Comprehensive Geographic Atrophy Market Analysis: Size, Share, and Industry Growth

Geographic Atrophy Market Revenue is expected to witness substantial growth over the coming years as new therapies and treatments emerge to meet the needs of the growing number of individuals affected by this advanced form of age-related macular degeneration (AMD). Geographic atrophy, marked by irreversible retinal cell damage leading to vision loss, has spurred the need for innovative solutions and the development of novel therapeutic approaches. The market report by SNS Insider sheds light on the rising incidence of geographic atrophy and examines key industry dynamics, emerging treatment modalities, and ongoing advancements in therapeutic interventions that are expected to reshape the landscape of GA management globally.

As aging populations increase globally, so too does the prevalence of geographic atrophy, driving robust growth in the GA market. The rise in geriatric populations worldwide, especially in developed regions like North America and Europe, has escalated the demand for effective treatments to manage GA's impact on patients' quality of life. This market analysis explores the latest breakthroughs in geographic atrophy treatment, such as complement inhibition and regenerative therapies, which are making promising strides toward slowing disease progression and preserving vision. Industry players are heavily investing in research and development to bring these advanced therapies to market, with several promising candidates in clinical trials.

The report further highlights the pivotal role of regulatory support, research funding, and collaborative efforts among pharmaceutical companies, research institutions, and healthcare providers to expedite the development and approval of new GA therapies. The growing need for early diagnosis and treatment of geographic atrophy has led to increased investment in advanced diagnostic technologies, which will be crucial in shaping the future of patient care and market growth in the GA industry.

Get Free Sample Report@ https://www.snsinsider.com/sample-request/2480

Key Drivers Fueling Geographic Atrophy Market Expansion

Technological Advancements in Diagnostics and Treatment Technological innovations have led to the development of advanced diagnostic tools and imaging modalities that aid in the early detection and accurate monitoring of GA progression. Enhanced diagnostics, such as optical coherence tomography (OCT) and fundus autofluorescence imaging, are vital in understanding the underlying disease mechanisms and assessing patient-specific treatment responses. The increased utilization of these cutting-edge diagnostic solutions by healthcare providers is anticipated to support the growth of the geographic atrophy market significantly.

Growing Focus on Complement Pathway Inhibition Complement pathway inhibition represents a promising therapeutic strategy in GA treatment. Research has identified that targeting specific components of the complement system can potentially slow the progression of geographic atrophy. Several companies are now focusing on developing therapies that act on these pathways, including ongoing clinical trials that are showing favorable results in delaying disease progression. Complement pathway inhibitors could become a major breakthrough in GA therapy, with approvals anticipated in the near future.

Increased R&D Investments and Strategic Partnerships Industry leaders are increasing their R&D investments to accelerate the development of novel GA therapies. In recent years, there has been a surge in strategic partnerships and collaborations, both within the pharmaceutical sector and with academic institutions, aimed at advancing GA research. These collaborations are helping to bridge knowledge gaps and expedite the development of new therapeutic candidates, pushing the market toward rapid growth. Moreover, public and private funding support has proven instrumental in advancing research and development activities, resulting in a growing pipeline of innovative treatments for GA.

Regional Insights and Competitive Landscape

The geographic atrophy market report from SNS Insider delves into regional trends, highlighting significant market share concentration in North America and Europe. These regions lead the market due to the high prevalence of age-related macular degeneration and greater healthcare expenditure on innovative treatments. The Asia-Pacific region, however, is projected to experience notable growth over the forecast period due to its expanding healthcare infrastructure, increased disease awareness, and rising demand for advanced therapeutic options.

With an increasingly competitive landscape, major pharmaceutical companies are working toward obtaining regulatory approvals for GA therapies and expanding their portfolios to capture the growing demand. Companies like Apellis Pharmaceuticals and Iveric Bio are at the forefront of this development, and the report provides a comprehensive analysis of their competitive strategies, product pipelines, and recent advancements in the GA market.

Market Outlook and Future Potential

The future of the geographic atrophy market looks promising, driven by the increasing incidence of GA, aging demographics, and continued advancements in therapeutic research. While challenges remain, such as the high cost of treatments and limited awareness in certain regions, the ongoing innovations in GA management, particularly in regenerative and gene therapies, are anticipated to create new avenues for growth in the market. The market is also poised to benefit from improved reimbursement policies and enhanced healthcare infrastructure, especially in emerging economies.

The report concludes by emphasizing the importance of continued research, stakeholder collaborations, and patient awareness initiatives in advancing GA treatment options. With a commitment to addressing the unmet needs of GA patients, the market is set for a transformative period that promises significant improvements in patient care and quality of life.

About Us: SNS Insider is one of the leading market research and consulting agencies that dominates the market research industry globally. Our company's aim is to give clients the knowledge they require in order to function in changing circumstances. In order to give you current, accurate market data, consumer insights, and opinions so that you can make decisions with confidence, we employ a variety of techniques, including surveys, video talks, and focus groups around the world.

Contact Us: Akash Anand – Head of Business Development & Strategy info@snsinsider.com Phone: +1-415-230-0044 (US) | +91-7798602273 (IND)

[retinography]

AMD: Geographic atrophy

In age-related macular degeneration (AMD) with geographic atrophy, fundus autofluorescence (FAF) is used to assess the extent of retinal pigment epithelium (RPE) loss. Areas of atrophy appear as hypoautofluorescent zones due to the absence of RPE, while hyperautofluorescent borders may indicate regions at risk for progression. FAF is essential for monitoring disease advancement and assessing the…

[dawntechnologies]

Parylene Coating: The Future of Optical Biointerfaces in Photonics

Parylene is a trade name for a generic polymer material that is characterized by rather useful properties. Parylene C and Parylene N are two of the most frequently used Parylenes Parylene C is appreciated for its high pinhole resistance and barrier characteristics and Parylene N is appreciated for that it is biocompatible and has low autofluorescence.

Source URL : https://relxnn.com/parylene-coating-the-future-of-optical-biointerfaces-in-photonics/

[divinejhonson]

The Future of Medical Retina: Trends to Watch in Eye Care

 As we progress into the future, the field of ophthalmology continues to evolve rapidly, especially in the specialized area of medical retina. With advancements in technology, research, and patient care, the landscape of eye care is transforming, leading to better outcomes for patients suffering from retinal diseases. In this article, we will explore the key trends shaping the future of medical retina, focusing on innovative treatments, technological advancements, and the importance of specialized care, particularly at the best eye hospital in Peshawar.

Understanding Medical Retina

Medical retina focuses on diagnosing and treating diseases affecting the retina, the light-sensitive layer of tissue at the back of the eye. Conditions like diabetic retinopathy, age-related macular degeneration (AMD), and retinal vein occlusion are prevalent, leading to vision impairment and, in some cases, blindness. The future of medical retina revolves around improving patient outcomes through cutting-edge technologies and personalized care.

1. Advancements in Imaging Technology

One of the most significant trends in medical retina is the advancement of imaging technology. High-resolution imaging techniques such as Optical Coherence Tomography (OCT) and fundus autofluorescence allow for detailed visualization of the retina. These technologies enable eye care professionals to diagnose conditions earlier and more accurately, which is crucial for effective treatment.

Optical Coherence Tomography (OCT)

OCT is a non-invasive imaging test that captures cross-sectional images of the retina. This technology has revolutionized how retinal diseases are diagnosed and monitored. With OCT, specialists can visualize the layers of the retina, allowing them to identify subtle changes that may indicate the onset of disease. The ability to detect these changes early significantly enhances treatment outcomes, particularly for conditions like diabetic retinopathy.

Fundus Autofluorescence

Fundus autofluorescence is another advanced imaging technique that provides valuable information about the health of the retina. This method captures images based on the natural fluorescence of certain molecules in the retina. It is especially useful in diagnosing and monitoring conditions like AMD and inherited retinal disorders.

2. Innovative Treatment Options

The future of medical retina is not just about diagnosis; it also encompasses innovative treatment options that are transforming patient care. Here are some promising advancements:

Gene Therapy

Gene therapy has emerged as a groundbreaking approach to treating retinal diseases, especially those with a genetic component. Treatments that target specific genetic mutations offer the potential to halt or even reverse the progression of diseases like retinitis pigmentosa and Leber congenital amaurosis. These therapies involve delivering healthy copies of genes directly into retinal cells, restoring their function.

Anti-VEGF Therapy

Anti-vascular endothelial growth factor (anti-VEGF) therapy has become a standard treatment for various retinal conditions, particularly wet AMD and diabetic macular edema. These injections help reduce fluid leakage and prevent abnormal blood vessel growth in the retina. Ongoing research aims to improve the effectiveness of these therapies and extend the duration between treatments.

Sustained Release Devices

The development of sustained-release devices for delivering medications directly to the retina is another exciting trend. These devices can provide long-term treatment without the need for frequent injections. For example, the implantable devices that release anti-VEGF drugs over several months could significantly improve patient compliance and outcomes.

3. Telemedicine and Remote Monitoring

The COVID-19 pandemic has accelerated the adoption of telemedicine across various medical fields, including ophthalmology. Telemedicine allows patients to receive care from the comfort of their homes, making it easier for those with mobility issues or those living in remote areas to access medical retina services.

Remote Monitoring Technologies

Remote monitoring technologies, such as smartphone apps and home monitoring devices, enable patients to track their vision and retinal health. For instance, patients with diabetic retinopathy can use apps to monitor their blood sugar levels and report changes in their vision. This real-time data can help ophthalmologists make timely interventions, ultimately preserving patients' vision.

4. Personalized Medicine

The future of medical retina is leaning towards personalized medicine, where treatments are tailored to the individual patient's needs. Advances in genetics and molecular biology allow healthcare providers to identify specific biomarkers associated with retinal diseases. This information can guide treatment decisions, ensuring that patients receive the most effective therapies based on their unique profiles.

Genetic Testing

Genetic testing is becoming increasingly important in medical retina. By identifying genetic mutations linked to specific conditions, specialists can better predict disease progression and tailor interventions. For example, patients with a genetic predisposition to AMD may benefit from early interventions and lifestyle modifications to slow down the disease's progression.

5. Multidisciplinary Approach to Patient Care

A holistic approach to patient care is gaining recognition in the field of medical retina. Collaboration among various healthcare professionals, including ophthalmologists, endocrinologists, nutritionists, and primary care physicians, is essential for managing complex conditions like diabetic retinopathy. By working together, these specialists can provide comprehensive care that addresses not only the eye health of patients but also their overall well-being.

6. Education and Awareness

Increasing public awareness about retinal diseases and the importance of regular eye examinations is crucial for early detection and treatment. Educational campaigns aimed at at-risk populations, particularly those with diabetes, can significantly impact patient outcomes.

Community Outreach Programs

Community outreach programs can help educate individuals about the signs and symptoms of retinal diseases, encouraging them to seek timely medical attention. Hospitals like Amanat Eye Hospital in Peshawar play a vital role in these initiatives, providing information on available services and the significance of regular eye check-ups.

Conclusion

The future of medical retina is filled with promise, driven by advancements in technology, innovative treatment options, and a patient-centered approach. As we look ahead, it is essential for patients and healthcare providers to stay informed about these trends to ensure the best possible outcomes for individuals affected by retinal diseases.

With the ongoing developments in imaging technology, treatment options, and personalized care, patients can expect better management of their conditions and improved quality of life. Facilities like Amanat Eye Hospital, recognized as the best eye hospital in Peshawar, are at the forefront of these advancements, ensuring that individuals have access to the latest in medical retina care.

[in-viro-veritas]

Autofluorescence, my old nemesis...

[donnierhettkoonce]

An Overview of Hydroxychloroquine Retinopathy

Hydroxychloroquine retinopathy is a serious side effect associated with prolonged use of hydroxychloroquine, a medication commonly used to treat autoimmune diseases like lupus and rheumatoid arthritis, as well as malaria. This condition primarily affects the retina, the light-sensitive tissue at the back of the eye, and can lead to irreversible vision loss if not detected and managed promptly.

Several factors contribute to an individual’s risk of developing hydroxychloroquine retinopathy, including the duration and dosage of use, age, weight, existing eye conditions, and kidney or liver disease. Symptoms may not be noticeable in the early stages, but can include blurred or distorted central vision, difficulty reading, color vision changes, and blind spots in the visual field as the condition progresses.

Early detection is crucial, and routine ophthalmologic screening is recommended for all patients on long-term hydroxychloroquine therapy. Diagnostic tools such as visual field testing, optical coherence tomography (OCT), and fundus autofluorescence (FAF) help identify retinal abnormalities.

Preventive measures include adherence to dosage guidelines, regular eye examinations, consideration of alternative medications for high-risk patients, and awareness of individual risk factors. By implementing these precautions, patients and healthcare providers can mitigate the risk of hydroxychloroquine retinopathy while maximizing the drug’s therapeutic benefits.

It’s important for healthcare providers to educate patients about the potential risks associated with hydroxychloroquine use, and to closely monitor their ocular health throughout the course of treatment. Additionally, patients should report any changes in vision or other symptoms promptly to their healthcare provider for further evaluation.