#Sry for the extreme delay

[jaylienpotter]

October 6th @prongsfoot-microfic prompt "blow" (I'm v late ik, I'm sorry)

Birthday

James sat at his desk studying - or trying to. He was in his last year at Loughborough University, studying sport science. Besides wanting be a professional athlete, he also decided on helping those in that area. He could be a sports psychologist, since he always had a passion for helping, or a coach. He had options and time. He just needed to focus.

His phone rang. 'Sirius' - scratch that plan.

They exchanged numbers after Black finished the commission of drawing Potter's dog, Padfoot. It was on the owner's phone screen. To his delight, Pads was also inspiration for a small ceramic sculpture, 'consider this extra as a thank you for your family's pottery lessons', Sirius had said.

"Hello?"

"Hey, it's Sirius."

"I know, I have your contact saved. And there aren't many Siriuses, I reckon." His smile was probably audible. The other boy had the same type of humour, which was comforting.

"I'm special like that. So uhm... My birthday's next week's Friday. I don't normally celebrate it but my friend Marlene insisted on doing something since we don't see each other much ever since I moved here. We're going to a bar, just us, her girlfriend and my ex."

"Your ex?" Perhaps he still had feelings for them? I mean, wasn't it awkward to be around an ex?

"Yeah, he's my best friend. Even after the breakup." He. If it hadn't been clear already, this was the confirmation that the hottest man James had ever met was, in fact, not straight.

"Oh, that's nice."

"So... Would you like to come? You don't have to, I understand that we haven't met long ago and you don't know my friends..."

"I'd love to!" Maybe he answered a tad too fast. But Black didn't seem to mind.

"Wicked! It's at The Bell Foundry. How does 8 PM sound?"

"Perfect!"

"Brill. A'ight, see ya then, mate. Oh, and don't get me anything." He definitely would.

"Bye, cheers."

Potter got there 15 minutes earlier. He was used to arriving first. When the birthday boy arrived, the tanned one blushed, heart skipping a beat. Fuck, he's bloody hot.

Glittery sheer crop top, his pierced nipples and chest tattoo visible, ripped black jeans with fishnets underneath, a buckled belt and chains, high heeled platform black boots, a choker that James wanted to pull, several silver necklaces and rings, and a glittery black smokey eye makeup with star rhinestones on the inner corners. His sharp features were even more noticeable with his hair in a messy bun.

"Like what you see?"

"Uh- I-" He composed himself and cleared his throat "Happy birthday!" The other man snorted but didn't press on the matter.

"Thank you. And thanks for coming."

"Thanks for the invitation. I know you told me not to but... I was never good with following orders." It was half true. He obeyed those he looked up to, like his parents and coach. Other than that? Mischief maker that looked too innocent for his own good.

He extended a black tote bag with a ribbon. "You really didn't need to-"

"I wanted to. Go on, open." Pale hands opened the bag, a bit confused. "It's empty?" To further confuse him, Jamie grinned.

"Oh silly me! It's backwards!" He exclaimed theatrically, took the bag and turned it inside out, revealing a Guns 'N' Roses logo. "There you go!"

"Bloody hell, this is great! You like them too??"

"Haven't heard much, to be honest. But you were wearing a graphic shirt the day we met and you have a quote of them on your Instagram so I figured you were a fan."

"That's actually so thoughtful, thanks." Good, he doesn't see it as creepy. You can relax now.

They hugged and pulled away after a whistle caught Sirius's attention.

"Happy birthday Starlight!"

"Moony!" He ran and hugged who Potter presumed was the ex. He was a tad intimidating. Very tall, neutral expression that could hide so much emotion, some few scars that indicated fights... Yet he was strangely attractive. He could see the appeal.

The brown haired lad hugged back, patting his friend's head. Potter couldn't help but feel jealous.

"Meet my new friend James! James, this is Remus."

"Hi, nice to meet you." Was the waving awkward?

"Likewise. Yer here early. I usually have to wait for ye." He had turned to his ex and swiftly put a cigarette between his chapped lips.

"Yeah, wanted to be with James before anyone else got here so he wouldn't be alone." Potter wasn't blushing. He wasn't. He was.

"Oh, thanks. That's nice of you."

"I'm always nice." The snicker coming from the tallest lad indicated otherwise. And from the little James already knew about Black, he was definitely very cheeky. "We should probably head inside. We'll catch a cold if we wait for Marlene."

"I dunno how ye two manage to be late everywhere. If ya weren't both gay, ya'd be a perfect match. Ye two go ahead, imma finish my smoke."

It had taken another half an hour before the last two members of the party arrived together. The mentioned Marlene was a short bubbly non-binary Filipino who was unapologetically loud. Their girlfriend Dorcas was more collected yet sociable. Her very dark skin looked amazing with the bright colours of her outfit.

Potter's nervousness eased after a while. They hit it all off well, albeit being ever so slightly jealous of 'Moony'.

"So what's the deal between ye two?" They both looked over to where the birthday boy was moving his hips to the music, next to a box dyed blonde head.

"Oh, we're just friends." Sadly. The tallest probably read his mind, giving him an unreadable look.

"I reckon he fancies ye. I recognise those eyes. Don't know ye but I'd say y'also do."

Saved by the bell, or rather, by the cake. "Happy birthday to you..." Dorcas had a cake in her hands, placing it next to James. Marlene was pulling their friend through the crowd.

"Happy birthday to you... Happy birthday dear Sirius..." All four were singing, some clubbers and bartenders joined, others didn't bother. Black seemed to love the attention he was getting.

"Happy birthday to you!" The grin. The eye twinkle that matched the top. He ought to celebrate his anniversary from now on.

"Make a wish!" Silver eyes shut as if concentrating very hard and the candles were blown. People cheered and went back to their lives.

"Thank you all for this. Really. Did you make it, Dorcas?"

"Yes I did. My famous chocolate fudge."

"You're amazing. A'ight, let's cut it before Moony starts drooling." They all laughed.

The cake was top tier. The music had been great. The environment was friendly and so were the people. All in all it was a very fun night, one like James hadn't had in a long time.

[mintjuliee]

I have covid 😓

[timetobeaghost]

Made up my mind about the fruit Pizza scene

I was always questioning what Mike really wanted to say, but am sure now he wanted to break up like El. She was clearly going to suggest being friends, and he was basically about to do the same, only that he was going to apologize and explain himself and emphasize how much he really DOES care about her, but not in that way, and will always be there for her, while she would have made it short and sweet.

Basically they were on exactly the same page and everything would be fine if Argyle had not interrupted. ARGHgyle! But he does interrupt and they enjoy getting to delay the somewhat difficult conversation and just be friends. Which Will of course interprets as true love and an outstanding example of heterosexuality. Oh, Will.

Mike was under the influence of the van scene, in which he got a lot of mixed messages, but one of them being that El needs him and he always will. But he was also still under the influence of the "Sometimes it's hard to tell the truth... because what if they don't like the truth" conversation and I think that was an extremely important one, putting him on the path of mustering the courage to tell the truth.

Then after the both conversations we have him reunite with El, deliriously happy that she is well and he found her. She makes it romantic, putting their foreheads together and cradling his face, while he breaks the romantic tension within seconds as per usual. (Which is what made El come to the conclusion that they really should be just friends)

Then there is the extremely interesting fact of his face falling and him looking between Will and El with a sad expression. That is the moment he really decided or realized that he can not love El "the way she wants him to". All leading up to his attempted confession in the Surfer Boys.

But all went to shit when El was in trouble and Will insisted that Mike must confess his love to El or she would literally DIE. Also Max would die and the world would end. Medium pressure.

WILLLLLL, no! Mike looks so desperate and pained because he already decided to be honest with El and that this would be the best for both of them. He is forced far back into the closet at gunpoint by the boy he is in love with. It's heartbreaking shit.

Disclaimer: All credit to the gif makers. Don't know their names nor would I dare to name them because many are probably milenvens. Sry..

[youngreckless]

32. "You could have died", for Malec pls? (hope I did it all right I'm new on tumbrl sry)

hello, you sweet pebble. i am extremely sorry for the delay, and thank you for being patient. 🌈 and you did it absolutely right, don't worry, i am still new to some of this stuff too. here you go, i hope you like it.

32. "You could have died." for malec (magnus/alec)

Alec released three arrows in quick succession; all of them hit bullseye. He closed his eye, nocked another one and shot. Bullseye.

He was in the Institute's training room, not because he needed to train, but because he missed it. He had not really been able to get any demon-fighting experience ever since becoming the Consul. Which did not mean that he was complaining about his new position, he just... missed the thrill of the action.

That is why he had come to the Institute, to spend some time with his bow and arrow. He nocked another arrow and shot it, but surprisingly, this one did not reach the target board. Alec only noticed it when he did not hear the familiar sound of the arrow hitting the board, which brought him out of the trance he was in.

He shook his head and was surprised to see Magnus standing between him and the board, holding the arrow Alec had just shot in his hand, a satisfied look on his face.

"Magnus! What are you doing?! You could have died!"

Magnus rolled his eyes. "Let's not be so dramatic now."

"Fine, but you could've been badly injured. You're immortal, you know, not invulnerable."

Magnus waved his hand dismissively in reply. "I would've been fine."

Alec glared at him.

"Seriously, Alexander. I have magic," said Magnus.

Alec sighed and walked over to Magnus. He leaned up to press a chaste kiss against his husband's lips but Magnus grabbed his face when he tried to pull away, and deepened the kiss. Alec kissed back with equal passion, if not more.

He hummed when they finally broke the kiss. By the Angel, he would never get tired of kissing Magnus. He gave one final peck to his husband and pulled away smiling. "What are you doing here, anyway?"

Magnus mirrored Alec's smile. His hand was still lightly caressing Alec's face when he replied, "It's dinner time."

"Already? Wow, I really lost track of time there. Where are the kids?"

"They ran straight to find your mother as soon as we portaled in."

Alec chuckled at that. Of course. Max and Rafe loved Maryse, and Maryse loved the kids. And even though Magnus and Alec loved their kids beyond measure, they rarely got time together, just the two of them. So it was a win-win for everyone.

Alec kissed Magnus again, and again; he couldn't help it. He wanted to savour the moment. Magnus clearly did not mind, though, for he kissed back with equal fervour.

In time, they would go back downstairs to find their kids. And they would all go to dinner, laughing and joking, with all the love in the world between them. But for now, this moment was for just the two of them, Magnus and Alec.

[fmdrohan]

hello, hi ! 👋🏽  i hope this introduction post finds everyone well. ♡ i’m no one new to these parts, but for those of you who don’t know me, you can call me alé. i’m 21+, use he/him pronouns, and i’m the current typist for aria’s main vocalist, nina seo, & now, i’m bringing you all yet another muse. so, without further delay, i’d love to introduce you to brand-new creation of mine called 𝖞𝖔𝖔 𝖗𝖔𝖍𝖆𝖓. he is knight’s main dancer, sub vocalist, and rapper, as well as their currently marketed “sexy” member. outside of his career, he’s honestly just one big douche-bag skater kid that seeks cheap thrills, and who wants to do nothing more than put a smile on your face... even if causes him trouble sometimes. more info about him is below the cut, so please like this to plot ! ♡ tumblr im’s or discord by request.✨ 

𝖍𝖎𝖘𝖙𝖔𝖗𝖞.

born in seoul, south korea, to a working class, christian family.

mom was a manager at a local restaurant, dad worked at a law firm.

both of them were busy all the time; they lived paycheck-to-paycheck.

he didn’t get much attention at home, so he sought it out at school.

there, he was known as a a huge “class clown” or “class daredevil.”

all he wanted was to put smiles on people’s faces and push limits.

did he end up in the principal’s office a lot? YES, that was his “brand.”

as he got older, his class clownery turned to pranks and thrill-seeking.

he also became HELLA interested in skateboarding and parkour lmao.

rohan felt as if he wasn’t living if he wasn’t on constantly on the edge.

he’s broken a few bones, endured tons of scrapes, but never gives in

this, obviously, didn’t please his mother and father... they were just busy.

too busy to reprimand him and care—rohan got away with a loooot of shit.

he didn’t start receiving discipline until he was scouted by bc entertainment.

despite his messy hair, distinct style, and overall vibe, he fit the bill somehow.

the two years he spent in practice changed him a little as a person; for better.

he found a new love in dancing, and he was naturally pretty great at it tbh.

his background made him extremely coordinated, flexible, and really strong.

his fearlessness made him bold enough to try out new moves or stunts.

two years later, he débuted as a main dancer and kept his impulses at bay.

this isn’t exactly what he saw himself doing in life, but he wasn’t mad at it.

over time, his image began to shift into sexier and “stud-lier” territory.

his outfits became more revealing and he was treated differently in public.

this... fucks him up a lot because it’s the exact opposite of how he is.

he’s just some skater / parkour punk that gives off dumb big bro vibes.

how on earth do they expect him to be portray and convey sexy 24/7?

as this is more of a “job” to him than passion, he does what they tell him to.

so long as his checks get signed and he accumulates wealth, he’s good.

𝖕𝖊𝖗𝖘𝖔𝖓𝖆𝖑𝖎𝖙𝖞.

as mentioned above, rohan gives off very douchey “big brother” vibes.

he’ll pick on you, tease you, pull pranks on you, etc. all in the name of fun.

he’ll go too far sometimes, and he’ll feel bad, but has “no regrets.” 😎  lol.

lowkey loooves anime and draws his own bad sketches; also into sci-fi.

underneath the dumb, douchebag exterior is just a big ol’ nerd, that’s all.

if you’re a cute guy, he’ll DEFINITELY flirt with you—taken or not taken lol.

he’s someone that hasn’t really let being an idol ruin his personal life tbh??

he still does whatever the fuck he wants within the constraints of his contract.

though, whenever he gets “bored,” he does things he’s not supposed to.

bc entertainment’s warned him of being careful not to injure himself, but...

i don’t really think he cares at this point in his career; he likes more freedom.

little random, but he loooves underground / indie rock music and rap, too.

one of those assholes that doesn’t really listen to any other idol music lmao.

to be fair though, he’s not really a massive fan of knight’s music either. 😁

he just sings or raps whatever they tell him to and rolls with the punches.

lowkey wants to take up tattooing and graffiti at some point, he’s that guy.

loooves to talk and meet new people, even if they find him a li’l annoying.

whenever he’s stressed, angry, upset, hungry... he shows it with aggression.

it’s never gotten intense much, but he’s not much of a sensitive “crier” yk.

there’s more of him for me to discover as time goes on, so that’s it for rn!

𝖕𝖑𝖔𝖙-𝖑𝖎𝖓𝖊𝖘.

no plots page yet, sry :/// i’ve been a little busy this week rip.

some ideas will be below though so lmk if they appeal to you!

one, he’s definitely known to ghost people, so... maybe your muse is one of ‘em? like, this would be locked to males in the lgbt community, but you know. give him some really awkward encounters to make him feel like shit for ditching you after tons of sweet talk. sad thing is, he’s aware of it, and it’s mostly because of his schedule, but also ??? he’s just having fun, you know. he doesn’t wanna get tied down to anything right now.

two, maybe that ONE GIRL he tried to fuck with at the beginning of his career. while he knows he’s always been attracted to men, i think he would’ve convinced himself to at least try it out with a woman to make sure he wasn’t also interested in them, too. he’s a MASSIVE flirt and sweet talker, definitely made her feel special, but like... it never really went anywhere because... he just wasn’t into it, but didn’t wanna make her mad lmao. clearly ended up in a break-up and it could be both an ugly or pretty ending imo idk.

three, i’d DIE if other muses in famed were super into skating, or anime, or really any of his interests, too, and they’ve formed a “club” of sorts? just your local band o’ dirtbags who get together and fuck shit up a little bit here and there. i feel like this could be open to anyone who shares any of his interests tbh ??? maybe closer to his age is preferred, but honestly, i’m here to discuss whatever! i’m all for compromises and shit tbh.

four, maybe someone’s he’s lowkey seeing ( male, male-presenting lock ) that he’s really vibing with, but he just can’t... feasibly reason why he should have a bf right now. he’s got a lot going on, so he’s kind of a flake, but at the same time, he doesn’t intend to be an asshole to them. they’re maybe one of the only people he’s “softer” with. can end up romantic or platonic depending on how they handle it together, me thinks!

five, a dance partner or two would be pretty cool tbh! he’s not a dancer first, so he likes to dance with other people who are passionate about it to learn from ‘em. he can handle his own with choreography, but doesn’t have his own distinct style, so he’d really appreciate all the help he can get in becoming better ig? all in the name of work!

six, maybe a person who fucking HAAAATES him because they find him really annoying and super douchey, so they just... avoid him at all costs. however, he kinda picks up on the fact that they avoid him, so he pesters them even worse, which doesn’t end well... ever. this is definitely more open to anyone tbh and we can plan as we go!

seven, flipside where it’s someone that he CAN’T stand because he finds them really persnickety, snobby, and rude as fuck. if there’s one thing he hates A LOT, it’s bratty rich kids with silver spoons in their mouths. like, if this was a “the outsiders” verse, he’d be a greaser 1000000%, not a soc. lmao. he’d butt-heads with this person a fuck-ton.

eight, however many guys wanna piece of him, he’s happy to have a catalogue of fwb’s he calls on. he’s definitely not someone who says no to a good time, and he can act like a perfect boyfriend if that’s what you want. sadly, this’ll always be a temporary game. any of these fwb’s can go in many directions and can be plotted out individually!

nine, the age old question... “who’s your ideal type?” under pressure, he said you as a joke ( because of this, it could be male or female ) and now, fans of your respective groups ship you two together all the time. it’s a little awkward, and you two haven’t really talked about it much, but here you are... at a shared event sitting next to each other. how do you handle it? what do you do? is it awkward or all in good fun?

ten, someone who kinda hates that he doesn’t really take being an “idol” all the serious. he’s got a lot of shit on his bucket list he wants to do and he doesn’t let fame fuck with that, so he’s careless. he didn’t go into this business because of passion, it’s just where he ended up. your muse doesn’t like that, and thinks people like him are lazy as fuck.

[spyder-incorporated]

Spyder Topic Season 1

Ok, so we all know Spyder to be the comic relief character in the show (even though we all know he deserves way more than that) P.S. This took way longer than I thought it would cuz I had to restart after accidentally deleting it instead of saving it to my drafts and then unreliable wifi while on vacation = big delay sry...

ANYWAY here is a list of all the times he suggested that he’s led a different life before becoming friends with Harris and Ryan during season 1 // the ultimate Spyder angst post

Btw this post is like 3 miles long so if your reading this on mobile.. I’m sorry.

Let's Call It MECH-X4!

Starting off in the first episode, this is a small moment in the first part... (episode is 45 minutes long and by first part I mean first 20 minutes)

Is no one going to acknowledge the fact that Spyder was just electrocuted to the point of passing out? Harris seemed to be concerned at the time but as soon as Spyder got back up to blast the monster, the entire ordeal was forgotten. Spyder may not seem to have any physical injuries but what about internally? 

If Spyder was knocked unconscious from the shock then that means that he will have some internal damage.. So why is no one concerned about it in the next scene?

Let's Get Some Air!: 

This is a gif and you can’t hear anything but it sounds like Spyder’s voice cracked at the end.. Whether or not he decided to show it, the mean comments he receives, right to his face, does in fact affect him.

What if Spyder’s been yelled at before? What if he’s accepted and can handle being yelled at by everyone he thinks will yell at him? Except for his friends.

Monster Harris just yelled in Spyder’s face that he was useless in helping the team. How can you assume he would just forget about it? Especially since Mark just called him a train wreck a little while ago as well. 

Why did Spyder try to fix his leg with duct tape? Did he not want to go to the hospital and get it professionally fixed so he tried to fix it himself but ended up making it worse? If I recall, his surgery had to be crowd funded. Did his family not have enough money to afford a hospital visit and he knew it? Is that why he tried to fix it himself as to not burden his parents with more money spending? Credit to @peanutryan who pointed this out to me! Thank you!

Let's Open The Monster Heart!: 

This was a very very very tiny moment but if you re-watch the episode and turn up the volume, it almost sounds like Spyder is in pain when Harper’s goons manhandle him. 

And look at his movement! It looks like he’s in pain. I might be nitpicking but I just don’t want to miss anything...

In this episode we learn that Spyder has a “crush” on Ryan and Marks mom and disapproves of Harper dating her, which the others think is gross. But what if it’s more psychological than that? What if back home, he doesn’t have a real relationship with his own mom and hardly sees her? So he subconsciously “likes” Grace Walker because she’s more invested in Ryan and Mark’s life which is something he doesn’t get from home?

Let’s Be Idiots!:

I know this was supposed to be a comedic sort of line but what if this was one of those criminal things he did as a bad boy before he met Ryan and Harris? I assume you would get in trouble for doing something like that? What is mooning someone mean? Look it up.

So children usually learn about pronouns during the years of preschool to about grade 2 (I couldn’t find an accurate grade sorry) So why doesn’t Spyder know what a pronoun is? 

Let’s Survive in the Woods!: 

Look at Spyder’s face though after he says “No, I didn’t.” He’s like “what are you talking about?” Like the aspect that he’s had a good life isn’t something that he thinks is possible...

For what reason would someone want to physically tie a rope around Spyder and keep him immobile?

Let's Get Our Robot Back!: 

So we know that Spyder has been to jail, but we don’t know whether or not he was in a cell serving a sentence or if he was visiting someone there. It would make sense both ways. So in later episodes we find out that Spyder knows how to pick locks which means he could have broken into places or things. What if one day he was caught and had to spend some time in a jail cell? It could go the other way though. We know Spyder has an unnamed mother but we don’t know anything about his father. What if he visits his dad in jail?

Later on we find out that Harris and Ryan met 18 months before the modern day in that episode. In the picture it looks like Harris and Ryan have been pretty good friends at that time so where is Spyder?

Let's Get Some Answers!:

The inner good side and the inner bad side of you. Spyder knows harris is on the good side so he tries to encourage him to act on the outside of the box with his inner Spyder. Would that mean that Spyder thinks of himself on the bad side, like he thinks he’s not a good person?

Let's Go Clubbing!

Spyder with the therapist talk! Later we learn Spyder actually does go therapy but why? Did he used to do the same thing that Ryan was doing but learned not to from his therapist?

Let's Get Leo!: 

This was one of those moments where Spyder showed how much he loves his friends.. If one of them is showing signs that they may leave (even if it’s not true) he will do everything in his power to make sure they stay. Like in Versus Miami! Almost as if he doesn’t just want his friends to stay but he needs them to stay.

Why does Spyder seem so calm when they could literally be crushed to death at anytime..?

Let's Destroy Some Ooze!

So this is the second time we see Spyder pretty upset (not counting season 2) in the series.. But it’s almost as if Spyder is saying it was an accident because he thinks his friends will blame him for destroying their school. Does he think they won’t see it as an accident?

Let's End This! Part One:

Ahh the famous Spyder scene. So here we learn Spyder goes to a therapist and even though he knows the world may be ending he’s so calm! What if Spyder could tell that Ryan was freaking out because he knows snapping at everyone is one of the signs of extreme stress and remained calm to reassure and calm Ryan down as best he could.

BONUS GIFS: Thought I’d add this in because this was a great scene with great character development. Spyder reassuring Ryan on his powers, knowing that they will win this battle!

AAAANNNDDD That’s Season 1!! Wow this took me wayyyy longer than I thought it would.. Sorry for the wait but here it is! 

This was way more angsty then I thought it would be omg

Did I miss something? Teeeellllll meeee         

Season 2 coming soon

[juniperpublisherscasestudies]

Juniper Publishers- Open Access Journal of Case Studies

Male with 45,X/46,X(r)Y Mosaicism due to a Ring Y Chromosome: A Case Report

Authored by Soumya Nagaraja

Abstract

The clinical, molecular, and cytogenetic findings in a boy with 45,X/46,X(r)Y mosaicism are described here. He has history of ring Y chromosome mosaicism diagnosed by amniocentesis performed due to advanced maternal age. He was treated for short stature and growth failure with growth hormone therapy. He was transferred to our care at 12 years of age. On presentation, he had a normal male phenotype, short stature, palpable testes and delayed sexual development. A post-natal karyotype and chromosomal SNP microarray revealed deletions of both terminal regions of the Y chromosome, consistent with the prenatal diagnosis of the ring Y chromosome. On karyotype, the presumptive ring Y chromosome was present in 29% of the cells and a single X chromosome was present in the other 71% of cells. FISH analysis demonstrated the presence of a ring Y chromosome in 37.1% of the cells. SHOX gene analysis revealed a complete gene deletion and is the likely cause of his short stature. He continued treatment with growth hormone and an aromatase inhibitor was added to delay growth plate fusion and to potentially increase his final adult height. The 45,X/46,X(r)Y mosaicism has been rarely reported. This mosaicism, due to the presence of the ring Y chromosome and depending upon on the presence or absence of the SRY gene can result in a wide spectrum of manifestations ranging from females with a Turner syndrome-like phenotype to phenotypic males. This karyotype can be associated with mixed gonadal dysgenesis in both boys and girls, depending on the tissue distribution of the 45,X and 46,X(r)Y cell lines. As the phenotype in mosaicism depends on the proportion of each cell line, analysis of other tissue types (other than lymphocytes) is needed to test the level of mosaicism and study its effect on the sexual differentiation and phenotype.z

Keywords: 45,X/46,X(r)Y mosaicism; Ring Y chromosome

Abbreviations: FISH: Fluorescent in Situ Hybridization; GH: Growth Hormone; ISS: Idiopathic Short Stature; SRY gene: Sex Determining Region Y; SHOX gene: Short Stature Homeobox Gene

Introduction

A ring chromosome is an extremely rare chromosomal aberration, which can occur in autosomes and sex chromosomes. The frequency of a ring chromosome in clinically detectable conceptions is 1/25,000 however, the incidence of the ring Y chromosome is unknown [1]. The formation of a ring Y chromosome involves terminal breakage in both chromosomal arms (p and q arms) and fusion of the resulting ends with loss of the deleted distal material (Figure 1). Because of the instability of a ring Y chromosome during mitosis, a second monosomic cell line is often present in these patients [2]. There are several mosaicisms reported with ring Y chromosomes including 45,X/46,XX/46X(r)Y, 45,X/46,XY/46,X(r)Y, 46,XX/47,XX(r) Y, 45,X/46,X(r)Y, 45,X/46,X(r)Y/46, X, dicr Y [3]. Patients with mosaic 45, X/46,X(r)Y karyotype, present with phenotypes ranging from females with Turner-like phenotypes, phenotypic males and females with mixed gonadal dysgenesis, to almost phenotypic normal males [4,5]. Variability of phenotype depends on the percentage of monosomic cells in different tissues and on the genetic material deleted during the formation of ring Y chromosome. There are very few published reports on 45,X/46, X(r)Y mosaicism. In most of the reported cases, patients have presented later in life due to infertility. Few of the reported cases in children have presented with clitoromegaly, chordee, cryptorchidism, hypospadias, and short stature. Here, we present the molecular cytogenetics of 45,X/46,X(r)Y mosaicism in a 12-year-old boy with normal male phenotype, chordee, short stature andpubertal delay.

Clinical Case Report

A 12-year-old boy of healthy and non-consanguinous parents was transferred to our care for management of short stature. He was being treated with growth hormone (GH) therapy for short stature and growth failure since 8 years of age. He was born at term after a normal pregnancy and delivery. He had history of ring Y chromosome mosaicism diagnosed by amniocentesis performed due to advanced maternal age. He had chorde eat birth and underwent surgical correction. His motor and mental development was normal. On examination his height was 139.5cm (8th centile). His weight was 37.4kg (32%), body mass index (BMI) was 19.2kg/m2 (70th centile). He was a well- nourished boy without any dysmorphic features. His external genitalia appeared predominantly male, his penis was 4.5cm, and right testis measured 2cc and the left testes 1cc with no axillary or pubic hair.

Initial amniocentesis showed 45,X karyotype in all cells. Prenatal ultrasound revealed a male phenotype and thus the specimen was re-evaluated by fluorescence in situ hybridization (FISH) analysis showing the presence of a ring Y chromosome. Since his prenatal chromosomal analysis was conflicting, we performed post-natal chromosomal analysis which revealed mosaicism 45,X/46,X(r)Y. On analysis, 71% of the cells showed a single X chromosome and 29% of the cells showed the presumptive ring Y chromosome. FISH for the Y chromosome showed that it was present in 37.1% of cells. Microarray showed deletion at both ends of the Y chromosome with a presumptive ring Y chromosome.

Analysis ofthe SHOX gene showed that there was a whole gene deletion. The SRY gene was present on the ring Y chromosome that contributed to his male phenotype. Ultrasound of the abdomen showed a horseshoe kidney but there was no gonadal dysgenesis. The echocardiogram and the EKG were normal. There were no other stigmata of Turner syndrome. Thyroid analysis showed TSH 9.47uIU/ml with normal thyroid hormone levels. Both thyroid peroxidase and antithyroglobulin antibodies were positive. Levothyroxine was added to GH therapy. He showed a good response to treatment and improvement in his height however, he continued to have delayed pubertal development. Since there was advancement of his bone age, anastrazole was added to delay growth plate fusion and to potentially increase his final adult height. Eventually, there was improvement in his gonadarche. At 14 years of age, his testicular size increased to 6cc bilaterally and complete biochemical analysis along with hormonal assessment was performed and was suggestive of hypergonadotrophic hypogonadism. At 16 years of age, semen analysis was done to test spermarche and he was noted to be azoospermic. These findings were consistent with deletion of the AZFa gene during the ring Y-chromosome formation. The AZFa gene is essential for spermatogenesis. The fertility potential in this patient cannot be determined at this point. A GnRH stimulation test and repeat semen analysis will be performed after discontinuing anastrazole and GH therapy

Prenatal chromosomal analysis and FISH studies on cells in the Amniotic fluid

In the initial cytogenetic evaluation of the amniotic fluid, an abnormal female karyotype was found in which all the cells examined contained a single X chromosome consistent with the diagnosis of Turner syndrome. Subsequent ultrasound of the fetus suggested a male phenotype. Therefore, FISH studies were performed on the interphase cells preserved from amniocentesis using the Vysis, sex chromosome probes: CEP1 which is specific for the X chromosome centromere, SRY which is specific for Yp11.3, and CEPY which is specific Yq12. These FISH studies showed a very low-level mosaicism for the hybridization to the Y chromosome probes. Two of the 100 cells showed male pattern of hybridization with the SRY probe. Two of the additional 100cells showed an unusual but positive hybridization to the Y centromere and long arm probes. Three cells contained small ring-shaped chromosomes that likely represented a ring Y chromosome. The SHOX gene was completely deleted on the Y chromosome but the SRY gene was present. The AZFa locus was deleted but the AZFb and AZFc loci were preserved. Its effect on the phenotype remains unexplained.

Post-natal chromosomal analysis

Post-natal chromosomal analysis showed 45,X/46,X(r)Y chromosome mosaicism. Both karyotype are shown below. The ring Y chromosome is indicated by the arrow (Figure 2 & 3).

FISH and microarray studies

Whole genome SNP microarray revealed both a terminal Yp and Yq deletion. There was a 1.02MB terminal deletion of the YPTER- P11.32 and 34.6MB terminal deletion of YQ11.223- QTER. This was consistent with ring Y chromosome. The presumptive ring Y chromosome was present in 29% of the cells and only a single X chromosome was present in 71% of the cells. Concurrent FISH analysis demonstrated the presence of a ring Y chromosome in 37.1% of the cells. Although the SHOX gene was deleted, the SRY gene was present on the ring Y chromosome that may have contributed to his male phenotype.

Semen analysis

At age 16, semen analyses were performed. Uncentrifuged and centrifuged semen specimens were examined microscopically and zero sperm cells were seen.

Discussion

Mosaicism is the presence of two or more populations of cells with different genotypes in one individual who has developed from a single fertilized egg [6]. Mosaicism can be seen in the somatic cells or the germline cells. In somatic mosaicism (Figure 4), different somatic cells of the body have more than one genotype. Different genotypes arise from same single fertilized egg cell due to mitotic errors at initial or later cleavages. In some rare cases, disorders of sexual development can be caused by mosaicism where some cells in the body have XX and other have XY chromosomes.

In germline mosaicism (Figure 4), the diploid germ cell precursors in the gonad (either sperm or oocytes) are heterogeneous. Some may have a mutation and some may be completely normal. A mosaic germline mutation is very important because it can be passed to offspring. Germline mosaicism can be observed with any inheritance pattern but it is most commonly seen with autosomal dominant and X linked disorders. Most individuals are unaware they possess germline mutations until they have an affected offspring [7].

Genetic mosaicism can be confused with chimerism in which two or more genotypes arise in one individual similarly to mosaicism. However, the two genotypes arise from the fusion of more than one fertilized zygote in the early stages of embryonic development, rather than from a mutation.

Here we present a male patient with a mosaic karyotype with a high percentage of monosomic 45,X cell line and small ring (Y) variants in his karyotype. The high percentage of the monosomic 45,X cell line in our patient, is seen due to the instability of the ring chromosome during mitosis. Molecular analysis showed deletion of the SHOX gene with the presence of the SRY gene on the ring Y chromosome, which explains his short stature and male phenotype, respectively.

Bettio et al. [8] indicated that phenotypic sex is determined by the presence of the 45,X vs. SRY-bearing cells and depends more on the number of copies of the SRY gene rather than on the percentage of 45,X cells, in the gonads. Thus in our patient, although the majority of cells have a 45,X karyotype, the phenotype is male and this could be explained by an enrichment of the 46,X(r)Y karyotype in the gonads.

The phenotypic spectrum of males with a 45,X/46,X(r)Y karyotype and bilaterally descended testes varies greatly from males with short stature and spermatogenic failure to males with normal stature and less severely affected spermatogenesis [9,10]. There has been a report of a 45,X/46,X(r)Y male with normal male phenotype, oligospermia, who fathered a son via in vitro fertilization [11]. Some of the case reports have reported subtle signs of Turner syndrome with male phenotype. Even with absence of Turner stigmata, cardiac and renal anomalies must be excluded in all such cases. It is also important to check for gonadal dysgenesis in these patients as it is associated with increased risk of gonadoblastoma.

The findings in this patient with 45,X/46,X(r)Y raise important questions about differing genetic mechanisms leading to the varied clinical spectrum of presentation. Previous findings from the prenatal amniocentesis prompted us to recheck the post-natal karyotype, SHOX gene and SRY gene in this patient, which would have been otherwise missed considering there were no associated characteristic features of a disease or syndrome in this patient except short stature, chordee and delayed puberty.

In pediatric endocrinology practice, deletions of the entire SHOX gene or mutations within or near this gene have been identified in some patients with short stature. However, there are many other genetic causes of short stature which go undiagnosed. These patients with short stature are grouped under the diagnosis of idiopathic short stature (ISS) and have no characteristic features of a known disease or syndrome. Cases like ours prompt us to think about the need for a thorough genetic evaluation to uncover the underlying cause of ISS so that appropriate treatment is provided. Performing karyotype, FISH and microarray can help to delineate the underlying genetic defect (including mosaicism and other chromosomal aberrations), which can affect not only growth and puberty, but also sexual development and maturation.

More case studies will give us a better understanding of how to treat children with short stature and hypogonadism as a result of mosaicism due to ring Y chromosome.

Conclusion

As the phenotypic presentation in mosaicism depends on the proportion of each cell line, analysis of other tissue types (other than lymphocytes) needs to be studied to test the level of mosaicism and to understand its effect on the sexual differentiation, phenotype, growth and development.

For more articles in Open Access Journal of Case Studies please click on: https://juniperpublishers.com/jojcs/index.php

[funnymalemodels]

It feels like forever haven't seen you on my dashboard lol! How are you there? So anw which Gods you want to see back to runway this SS18?

…hi dear anonymous…:D…awwwwwwww…hahaha..XD….yeah i feel the same…XD….awww….i’m fine…:’)…i didn’t post much coz i’m strugelling for the lack of decent work of GODS!!….*angry tears*…..but thanks!!!….u r vry nice!!..;)….abt ur question…..a lot…but so far ..i’m losing hope coz….most of them r busy….or probably didn’t go to fw….:’(…however i’m rly excitng to see Elijah!!!!!!!!!!!!!!!!!!!!….*screams*…he’s back!!…finally!!!!…and i swear i’m praying coz hope he bokeed the shows i like!!!!!….*tears*….i can’t wait to see him!!!…

…and Evan!!!!!!!!!!!!!!!!!!!!!!!!...*hysteria*!!….i love him…i mean …i’m crying ..sry coz he’s a real and total GOD!!!!!!!!!…*screams*.

…..then babyshark….Tim!!!!!!!....*sigh*….i was extremely happy to see him at Prada last season…..and for me if i saw him again i’ll die!!!!!!!….i rly miss him..:’(..…also  i want to see Nathan S…and Simone!!..…i wish see Simon…but i know .. it will not happen…:’(……and i know i’m asking too much but i’m praying to see Baptiste without beard!!!….….but…in general… i’m always praying for all the gods u see on my blog coz i wish them book as many shows as they can….and get payed extremely well…..XD….anyway….i can’t wait to see this ssfw!!!…*screams*….HUGE THANKS 4 asking and have an incredible day and life!!!….;)!!!

ps:…soz 4 the delay….;)!!!

[hishotandhaywireheart]

Survey from Esther~

1: 6 of the songs you listen to most? Janina Gavankar - Don't Look Down The Irrepressibles - Two Men In Love The Irrepressibles - The Arrow Flor - Warm Blood Shearwater - Animal Life Bastille - Laura Palmer 2: If you could meet anyone on this earth, who would it be? I'm overwhelmed by this question and can't pick a single answer, sorry lol. This is like asking me what my favorite Pokémon is. 3: Grab the book nearest to you, turn to page 23, give me line 17. I'm in a dog boarding facility's kitchen at the moment, no books in sight, I'm afraid. But I am reading the first book of the Raven Cycle series in audio form, if that helps 4: What do you think about most? What a question. Art? Animals? 5: What does your latest text message from someone else say? It's my boyfriend saying he's going to sleep :3 6: Do you sleep with or without clothes on? *Gamagoori voice* I sleep in the nude 7: What’s your strangest talent? Even though I am not a smoker [unless you count a couple of hookahs per year], I can tell the brand of a cigarette by the smell of the smoke. The smell on someone's clothes, I know immediately what brand it is. Mixture of childhood exposure, sensory sensitivity, and the jobs I've worked lol. 8: Girls… (finish the sentence); Boys… (finish the sentence) Girls are powerful. Boys are powerful. 9: Ever had a poem or song written about you? I dunno. Maybe? 10: When is the last time you played the air guitar? Uhh. Not a thing that occurs to me to do, to be honest lol 11: Do you have any strange phobias? Big phobia of hypodermic needles, or generally anything like a splinter, tiny shard of glass or hook getting underneath my skin. Phobic of getting pregnant. I guess those are weird. 12: Ever stuck a foreign object up your nose? Uhhh probably? 13: What’s your religion? What a complicated question lol. Simple answer: I'm pagan. But there is literally nothing simple about my endless thoughts about spirituality 14: If you are outside, what are you most likely doing? Taking a walk in the woods, or sitting by a stream or body of water [water sources are especially sacred parts of nature for me] 15: Do you prefer to be behind the camera or in front of it? Both. But I love taking photos and nobody really takes any photos of me. So I guess behind. Especially when it's behind a Polaroid. 16: Simple but extremely complex. Favorite band? Shearwater. Honorable mention to the Irrepressibles. 17: What was the last lie you told? Fuck if I know 18: Do you believe in karma? Not really. But as always I believe in the awesome power of the brain. 19: What does your URL mean? It's my name with "Irrepressible" after it, in the style of Jamie Irrepressible, the vocalist of, well, you can probably guess which band. 20: What is your greatest weakness; your greatest strength? Weakness - I'm extremely prone to gaslighting and self doubt about my own reality. Strength - I might not be a fan of abrupt change, but I am very adaptable, given time to adjust. 21: Who is your celebrity crush? None 22: Have you ever gone skinny dipping? No, sadly. Remember what I said about my irrational fear of stepping on hooks or sharp objects? Interferes with my sacred need to swim in every lake I see. 23: How do you vent your anger? Ideally, art. Drawing, writing, even recording myself ranting to my microphone about it. 24: Do you have a collection of anything? I collect retro Pokémon merchandise and certain old video games. :> this makes me sound like a massive genwunner but rest assured, it's just an Aesthetic™ 25: Do you prefer talking on the phone or video chatting online? Text, to be honest. Us auties generally do better communicating in text based media. Skype calls are useful at times but I've always found them too awkward with delays and such to use reliably. 26: Are you happy with the person you’ve become? Hmmm. Tentatively, yes? 27: What’s a sound you hate; sound you love? Sound I hate: male voices shouting. Sound I love: music bouncing off walls and becoming ethereal and far away. 28: What’s your biggest “what if”? What if I don't understand what this question is asking me? 29: Do you believe in ghosts? How about aliens? Ghosts, no. I talk about them a lot though because the idea of them is dear and important to me, but literally, no, I have no belief in some vaporized version of your personality that goes on after death. Aliens, I assume are a matter of inevitability. But I don't believe we will probably ever find or meet them in the foreseeable future. Humans think they are much more fascinating than they actually are. Aliens are not crawling all over themselves to build technology just to come fly over to our house and meet us. Sry 30: Stick your right arm out; what do you touch first? Do the same with your left arm. Air both times lol 31: Smell the air. What do you smell? The heat coming on at work because morning is here. Faint dog poop smell. Gonna have to tidy that up lol 32: What’s the worst place you have ever been to? Buttfuck nowhere, West Virginia 33: Choose: East Coast or West Coast? East Coast, if we're talking america 34: Most attractive singer of your opposite gender? I don't have an opposite gender 35: To you, what is the meaning of life? To create something meaningful and beautiful, and to enjoy myself to the fullest while helping others whenever possible 36: Define Art. If it makes you think about whether or not it's art, then it's art. 37: Do you believe in luck? Uhh. Like as an actual outside force that decides whether good or bad stuff will happen to me, no. 38: What’s the weather like right now? Coldddd 39: What time is it? 6 am. Time to get off work! 40: Do you drive? If so, have you ever crashed? Yes, I drive, don't love it but glad I am able. No, but I did run over a bin once 41: What was the last book you read? A book about the history of heterosexuality as a concept, fascinating read actually 42: Do you like the smell of gasoline? As a kid I liked it but it's kinda gross now 43: Do you have any nicknames? Many 44: What was the last film you saw? Can't remember a film rn but I am currently watching The Story Of Film which I CANNOT recommend enough, it is a documentary series about, well, the history of cinema and even if you don't care about movie making... It will absorb you completely and make you not only care but be totally fascinated. I adore it. 45: What’s the worst injury you’ve ever had? You know, I've been really lucky. Never broken bone or needed stitches or even like, been to the hospital, lol. I think I chipped my shin bone on a brick stair once. And a couple years ago I missed a stair going down and sprained my ankle, and my work refused to let me spend any time off my feet so I was crying from pain in front of customers lol. But it healed fast once I had a brace. No problem. 46: Have you ever caught a butterfly? I dunno. I was taught as a child that if you touch their wing dust even a little, they will lose their flight and die. So probably I haven't. Even though I now know this isn't quite true. 47: Do you have any obsessions right now? The Story of Film, some bands, getting sucked back deep into my lifelong love Pokémon again lol 48: What’s your sexual orientation? Pansexual, or: Why Are Gender And Genital Shape Our Main Social Indicator Of Romantic Or Sexual Preferences, Of All Things, That's Really Weird And I Can't Relate, Please Save Me From This Bizarro World 49: Ever had a rumour spread about you? Oh yes, plenty back in school 50: Do you believe in magic? Ahh. I believe in the power of will. I believe in the ability to make your own life full of magic via willpower. I believe in the harmless use of willpower to try and cause a change in your environment. I believe the force exercised by children known dismissively as "imagination" have incredible power to influence the mind and soul. I have no belief in a metaphysical force in the universe called "magic" that could describe basically anything and everything unknown to current science. If you ask me flat-out, I will say yes, I believe in magic. But this is more of what I mean. I don't believe in "magic", except that I do. Adamantly. 51: Do you tend to hold grudges against people who have done you wrong? I do not tend to forget. Forgive is a matter of situation. But I don't forget. And I am quite the talented ice prince when it comes to freezing someone entirely out of my life. This includes immediate family. I only speak to my little sister out of my entire family. 52: What is your astrological sign? Taurus-Gemini cusp! 53: Do you save money or spend it? Spend :T 54: What’s the last thing you purchased? Bread, milk and a couple very cheap, very pink, very glittery nail polishes. My weakness. 55: Love or lust? Yes. 56: In a relationship? Yep 57: How many relationships have you had? Uhh... Many? Serious, deep romantic relationships, which I suspect is your real question: three. 58: Can you touch your nose with your tongue? Nope 59: Where were you yesterday? Home, and briefly out at the store. 60: Is there anything pink within 10 feet of you? My nails are pink and glittery. 61: Are you wearing socks right now? Yep. I wear two pairs to work because my super comfy work shoes are just the tiniest bit too big. 62: What’s your favourite animal? You asked the impossible question. Today, your answer is: praying mantis. Specifically praying mantis godmothers. Ask me again in two hours for an entirely different answer. 63: What is your secret weapon to get someone to like you? Sorry, too socially awkward for this prompt 64: Where is your best friend? In bed, it is very late and/or early. 65: Give me your top 5 favourite blogs on Tumblr. Don't really have favorites? Just people and stuff I like. 66: What is your heritage? Whitey white. Scots Irish, English, a bit of German, and like everything else you can expect from a family that's been in America about as long as a white devil could possibly be. My mother is a hobbyist genealogist, so this isn't just typical white folks bullshit, I'm vaguely more educated on my roots. I am in fact a distant cousin of notable American politician of the 19th century, Henry Clay. 67: What were you doing last night at 12AM? Playing Pokémon Blue! Beating the game for literally the first time ever! 68: What do you think is Satan’s last name? Uh. Um. Oh god, I don't know lmao 69: Be honest. Ever gotten yourself off? Yes, everyone has, normalize it 70: Are you the kind of friend you would want to have as a friend? Hmmm. Yes. 71: You are walking down the street on your way to work. There is a dog drowning in the canal on the side of the street. Your boss has told you if you are late one more time you get fired. What do you do? Lose my job bitches, and maybe hope to go viral and boost my chances of getting a new job for doing this thing lol 72: You are at the doctor’s office and she has just informed you that you have approximately one month to live. a) Do you tell anyone/everyone you are going to die? b) What do you do with your remaining days? c) Would you be afraid? A-Maybe. At least a few people. B-Travel, make good art, write my will, get my affairs in order. C- Yes, for a while. 73: You can only have one of these things; trust or love. Trust obvs 74: What’s a song that always makes you happy when you hear it? Many. First one that comes to mind is Empire by Jukebox the Ghost 75: What are the last four digits in your cell phone number? Nope 76: In your opinion, what makes a great relationship? Being best friends and trying to understand one another and willing to be open and honest, no ego in the way 77: How can I win your heart? Buy me sushi. 78: Can insanity bring on more creativity? Yes. 79: What is the single best decision you have made in your life so far? To not have children. 80: What size shoes do you wear? American size seven in men's, nine in women's. Sometimes half a size up or down. 81: What would you want to be written on your tombstone? No idea. I should get thinking on that. 82: What is your favourite word? Don't really have one favorite tbh 83: Give me the first thing that comes to mind when you hear the word; heart. Glowing lights, core imagery 84: What is a saying you say a lot? Hell yeah 85: What’s the last song you listened to? Maxiimo Park - Going Missing 86: Basic question; what’s your favourite colour/colours? Long story short: indigo. 87: What is your current desktop picture? Some Pokémon, I forget which. 88: If you could press a button and make anyone in the world instantaneously explode, who would it be? A random white supremacist, maybe, but honestly, I probably wouldn't press it 89: What would be a question you’d be afraid to tell the truth on? No idea rn 90: One night you wake up because you heard a noise. You turn on the light to find that you are surrounded by MUMMIES. The mummies aren’t really doing anything, they’re just standing around your bed. What do you do? I'd be quite disturbed because I cut my teeth on Ocarina of Time and was very creeped out by ReDeads 91: You accidentally eat some radioactive vegetables. They were good, and what’s even cooler is that they endow you with the super-power of your choice! What is that power? Shapeshifting. Always my answer. Covers being an animal, or being a child, or flying, or swimming. 92: You can re-live any point of time in your life. The time-span can only be a half-hour, though. What half-hour of your past would you like to experience again? First thought is a marching band performance from high school. Second thought is back in that car in the vast moonlit Utah desert. 93: You can erase any horrible experience from your past. What will it be? Bad math teachers. Gave me a complex about math and didn't improve me as a person in any way like most of the others did. 94: You have the opportunity to sleep with the music-celebrity of your choice. Who would it be? No interest 95: You just got a free plane ticket to anywhere. You have to depart right now. Where are you gonna go? Japan motherfuckers 96: Do you have any relatives in jail? Not that I'm aware of but I couldn't care less tbh 97: Have you ever thrown up in the car? If I have, I must have been very small. 98: Ever been on a plane? Yep, just twice. 99: If the whole world were listening to you right now, what would you say? If they actually absorbed what I had to say? Love yourselves. Love each other. Endeavor to understand each other. Try to figure out what you were taught wrong about yourself and your fellow humans, and unlearn those things. Embrace humanity in all its diversity. Open up and be vulnerable.

[w0wls]

Coms will resume tmrw

Sry fir any delays. New jobs is extremely overwhelming but today was last day this week so i will be good to continue tmrw!