#Pipette tip

[microlitseo]

Common Pipetting Mistakes & How to Avoid Them

Pipetting is one common task that’s performed in many laboratories, essential for ensuring precise and accurate results in experiments. However, even experienced scientists can fall prey to common pipetting mistakes that can compromise their work. After learning the necessary pitfalls and how to avoid them it will become significant to maintain the integrity of your data. Here are some common pipetting mistakes and tips on how to avoid them. 

1. Incorrect Selection of Pipette 

Mistake: Using the wrong type of pipette for your application can lead to inaccuracies. For instance, using a large-volume pipette to measure small volumes can result in significant errors. 

Solution: Always select a pipette that is appropriate for the volume you need to dispense. For small volumes, use a micropipette technology that’s designed for that range. Check the pipette’s specifications to ensure it matches your requirements. 

2. Not Pre-Wetting the Pipette Tip

Mistake: Failing to pre-wet the pipette tip before use can lead to inconsistent volumes due to evaporation or retention of liquid in the tip. 

Solution: Pre-wet the pipette tip by aspirating and dispensing the liquid several times before taking your final measurement. This helps to condition the tip and ensures consistent volume delivery.

3. Incorrect Pipetting Angle

Mistake: Holding the pipette at an incorrect angle while aspirating or liquid handling can cause air bubbles and inaccurate measurements.

Solution: Hold the pipette vertically (90 degrees) when aspirating liquids and at a 45-degree angle when dispensing. This minimizes air bubble formation and ensures accurate volume transfer. 

4. Plunging Too Quickly

Mistake: Rapidly depressing and releasing the plunger can introduce air bubbles into the sample, leading to inaccurate measurements.

Solution: Operate the plunger slowly and steadily. When aspirating, depress the plunger to the first stop, immerse the tip in the liquid, and release the plunger slowly. Similarly, dispense the liquid smoothly and consistently.

5. Immersing the Tip Too Deeply

Mistake: Immersing the pipette tip too deeply into the liquid can cause excess liquid to adhere to the outside of the tip, leading to volume errors.

Solution: Only immerse the tip 2-3 mm below the liquid surface when aspirating. For larger volumes, immerse slightly deeper, but avoid submerging the entire tip.

6. Using the Wrong Tip

Mistake: Using non-compatible or low-quality tips can cause inaccurate measurements and compromise the integrity of your pipette.

Solution: Always use high-quality, compatible tips recommended by the pipette manufacturer. Ensure the tips fit securely and are free from defects.

7. Not Using the Reverse Pipetting Technique for Viscous Liquids

Mistake: Pipetting viscous or foamy liquids with the standard pipetting technique can result in inaccurate volumes due to retention of liquid in the tip.

Solution: Use the reverse pipetting technique for viscous or foamy liquids. Aspirate more than the desired volume and dispense the exact amount needed. This technique helps to minimize errors and improve accuracy.

8. Inconsistent Pipetting Pressure

Mistake: Applying inconsistent pressure on the plunger can lead to variability in the volumes dispensed.

Solution: Maintain a consistent, gentle pressure on the plunger. Practice pipetting with a steady hand to develop muscle memory for consistent pressure application.

9. Neglecting Regular Calibration

Mistake: Failing to pipettes calibration regularly can result in inaccurate measurements due to wear and tear or mechanical issues.

Solution: Schedule regular calibration and maintenance checks for your pipettes. Follow the manufacturer’s guidelines and document calibration results to ensure accuracy over time.

10. Ignoring Environmental Factors

Mistake: Environmental factors such as temperature and humidity can affect pipetting accuracy, especially for volatile liquids.

Solution: Perform pipetting in a controlled environment where temperature and humidity are stable. Allow liquids to equilibrate to room temperature before pipetting.

Conclusion

Accurate pipetting is crucial for reliable experimental results. However, by being aware of common pipetting mistakes and following best practices, you can improve your pipetting technique and ensure the accuracy and reproducibility of your work. Regular training and practice, combined with proper maintenance of equipment, will help you avoid these pitfalls and achieve precise results in your laboratory experiments.

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Brief Introductions of Pipette Tips

Pipette tips are an indispensable accessory for pipettes. In some famous theories, the quality of the tip also is very important in the accuracy of pipetting, and its significance is no less than the pipette itself. Below is a brief introduction to pipette tips.

Brief Introductions of Pipette Tips:

Material of the pipette tip:

High-quality tips are generally made of natural polypropylene.

In addition, pipette tip material often with small amounts of additives. The more common additives are:

Color material. The common blue tips (1000ul) and yellow tips (200ul) on the market are with corresponding color-developing materials in polypropylene (as a user, you must hope that it is a high-quality color masterbatch, not low-quality) cost industrial pigments. )

Release agent. Mainly used to help the tip release quickly after molding. Due to the high production process requirements, often rare for pipette tips without additives are on the market, so our users can only choose pipette tips with fewer additives when choosing.

Classification of pipette tips:

1. Filtered pipette tip

There is a filter (usually white) on top of the filter. Such filter elements are typically made of polypropylene, similar to the filter structure of a cigarette.

Due to the existence of this filter element, the pipetted sample cannot enter the inside of the pipette, thus protecting the parts of the pipette from contamination and corrosion, and more importantly, it can also ensure that there will be no cross-contamination between samples.

Therefore,  filtered pipette tips are also an important tool for pipetting volatile and corrosive samples. Of course, the price of filter tips is relatively high compared to general tips, and the domestic market share is very low.

In addition, there are few domestic suction heads with filter elements, and their filtering effect is not satisfactory.

2. Sterile pipette tip

Hawach company provides the sterile pipette tip, which has all sizes, like 200μL, etc. And all of the sterile pipette tip with high-quality medical-grade polypropylene (PP) material;

The tips with high-temperature resistance, no deformation after high temperature, and high-pressure sterilization

3. Special tips.

Designed special tips are tips for special samples or special applications.

There are mainly the following categories:

The first category is gel tips (also known as dispensing tips): the tips of tips are either flat or very thin and are for electrophoresis experiments;

And the second category is the wide-mouth suction Tip: The diameter of the tip is larger than that of the ordinary tip, which is mainly for removing macromolecular solutions such as proteins;

Then the third type is the low-adsorption tip: the inner wall of these tips reduces the surface adsorption force, which removes viscous liquids;

The fourth type is the extension tip: mainly used to take liquids in containers such as volumetric flasks;

The fifth type is the outer piston tip: mainly used for special outer piston pipettes and pipettes on the continuous dispenser.

Features of pipette tip:

1. What are pipette tips used for:

First, the professionalism of the tips. Certain series of pipettes from certain brands can only use their own standard tips and cannot use other tips. For example, Rainin’s multichannel pipettes must use their own LTS tips;

The second is fit. The most common situation is that a pipette can use multiple tips, but the pipetting effect varies from tip to tip.

Third, should adjust the tip and pipette range. And the tip volume should be greater than or equal to the maximum pipette range. For example, a pipette with a maximum range of 20ul, 100ul and 200ul can use a 200ul tip;

Fourth, the characteristics of large-capacity pipette (5ml, 10ml, 20ml) tips. Large pipettes from the major pipette brands often require their standard tips, and replacements are difficult to find.

Therefore, if we need to buy tips, should tell the supplier which tip model we apply or at least the brand and capacity of the pipette we use.

2. Biological contamination

More and more users now require tips that are free of RNase, DNase, DNA, pyrogen, and ATP contamination.

And autoclaved tips are not completely guaranteed to be free of biological contamination.

Hawach company ensures there is no biological contamination, and gamma-ray treatments to produced.

There are also introduces about the function of disposable pipette tips, what is sterile filtered pipette tips, etc.

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[heliocentral]

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[hunters-angel]

tomorrow i'm working with a new research group for the first time, i am vibrating with anxiety

[fungi-maestro]

Lab season once again so that means it's time to draw the horrible slug that I can hear whispering in my ear when I fuck up my work

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no matter where i am, if i have to do any type of writing under pressure from an upcoming deadline, i WILL preferentially clean or organise the space in which i am currently existing.

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my favorite thing about tlou episode 7 is when they’re at the college and ellie is poking around and says “there were definitely doctors here” and she’s looking at…an open (miraculously unspilled, like all 96 are still in there, HOW) box of pipette tips under a weird magnifying glass thing

[accumax]

Different Types of Pipette Tips and When to Use Them

Like deciding what to wear for the day, walking into a laboratory means you have to choose the right pipette tips for your experiments. This is about precision in avoiding mistakes and contamination rather than personal preferences when working in a lab. There are various types of tips, each designed with a specific application in mind.

Below is an ultimate guide on how to make sense of different kinds of pipette tips and their uses.

1. Non-Sterile vs. Sterile Tips

Non-Sterile Tips: These can be used for general laboratory procedures where sterility is not necessary. Using non-sterile tips may save money if you are dealing with non-sensitive samples or performing routine tasks.

Sterile Tips:

Required when doing tests where contamination would lead to compromised results, such as in microbiology, molecular biology or clinical settings. Such tips are pre-sterilized and certified free from contaminants like RNase, DNase, endotoxins etc. Although it might seem appealing to autoclave non-sterile tips onto sterile ones, inconsistent outcomes may occur because autoclaving does not have quality control measures.

2. Filter Tips

Filter Tips They are designed in such a way that prevents aerosols and liquids from entering the pipette body and thus safeguarding your samples as well as the pipette. Whenever you are dealing with volatile substances, corrosives or infectious materials it is important to use them. In some cases like PCR experiments where there is risk of contamination leading to false results, presence of filter tips is compulsory.

3. Long Tips

Long tips can get to the bottom of narrow tubes and deepwell plates without causing contamination towards the pipette’s shaft. In case you are working in a laboratory where you transfer liquids from tall containers, it is advisable to use long tips that maintain sterility and accuracy.

4. Short Tips

If you have short tips, ergonomic pipetting will be made possible, and hence there will be less strain on your arm or hand especially if you are using small well plates or multichannel pipettes. By sitting closer to the bench, they ensure exact pipetting making them the best for such fast robots.

5. Low Retention Tips

Low retention tips refer to those that comprise of special polypropylene blend or else coated with a hydrophobic surface intended for minimizing liquid retention. It is particularly essential when handling viscous or low surface tension liquids like concentrated protein or DNA solutions; this kind of tips ensures maximum sample recovery as well as accurate pipetting which may influence sensitive assays.

6. Wide Bore Tips

In delicate specimens like cell suspensions, standard ones can shear cells mainly due to their small opening; while wide bore tips have bigger holes thus providing easy passage for the solution through them hence preventing damage among other things when dealing with delicate samples such as cells in suspension or very thick liquids.

What Counts Concerning Quality and Fit

Quality of the tips that you get is as important as choosing the right type of tip for your pipette. This is because, by selecting poorly fitting or low quality tips, you risk taking inaccurate measures, adding to the strain of pipetting and contaminating samples in the process. Thus, it is advised that a high-quality tip should be compatible with your pipette for better outcomes and accurate results. Keep in mind therefore, how well the tips fit into your pipette so as to ensure consistency in lab work.

The importance of selecting the correct pipette tip does not depend on someone’s liking but rather impacts on getting reliable results. In order to ensure successful and efficient lab work it is necessary to identify various types of pipette tips and their applications. This blog originally posted here:  Different Types of Pipette Tips and When to Use Them

[microlitseo]

How to Select the Right Pipette Tip: Step-by-Step Guide?

When working inside a laboratory setting, accuracy and precision of liquid handling are considerably of great importance for reliable results. One of the key components that play a significant role in this process is the pipette tip. Thus, choosing the right pipette tip can influence the outcome of your experiments by ensuring that the volumes are transferred accurately and contamination is minimized. This step-by-step guide will help you navigate the selection process to find the perfect pipette tip for your needs. 

Step 1: Understand Your Pipetting Needs

Before selecting a pipette tip, it's essential to understand the specific requirements of your experiments. Consider the following questions:

Volume range are you working with: Different pipette tips are designed for different volume ranges. Ensure the tip you select matches the volume capacity of your pipette.

Type of liquid you are handling: Viscous, volatile, or corrosive liquids may require specialized tips.

Working with sensitive samples: For applications like PCR, RNA/DNA work, or cell culture, contamination prevention is critical.

Step 2: Choose the Correct Tip Size

Pipette tips come in various sizes, typically indicated by their volume capacity. Common sizes include:

10 µL tips for very small volumes.

200 µL tips for general laboratory use.

1000 µL tips for larger volumes.

Make sure to select a tip that fits within the range of your pipette. Using an inappropriate tip size can lead to inaccurate measurements and potential damage to the pipette.

Step 3: Consider Tip Compatibility

Not all pipette tips are universal. Ensure that the tips you select are compatible with your pipette brand and model. Many manufacturers provide compatibility charts or guidelines to help you match the correct tips with your pipettes. Using incompatible tips can lead to poor fitting, leakage, and inaccurate results.

Step 4: Evaluate Tip Quality

The quality of the pipette tip can significantly impact your pipetting accuracy and precision. Look for the following features:

Smooth Surface: High-quality tips have a smooth surface that ensures accurate volume delivery and prevents liquid displacement. 

Low Retention: Some tips are specifically designed to minimize sample retention, which is crucial when working with valuable or limited samples.

Certification: For sensitive applications, choose tips that are certified DNase/RNase-free and pyrogen-free.

Step 5: Decide on Filtered vs. Non-Filtered Tips

Filtered tips have a barrier that prevents aerosols and liquid handling from contaminating the pipette. This is particularly important for:

Preventing Contamination: When working with biological samples or hazardous materials.

Protecting the Pipette: Filtered tips can protect the pipette from corrosive or volatile substances.

Sensitive Applications: Such as molecular biology or microbiology, where contamination can severely affect results.

Non-filtered tips are suitable as general laboratory equipment for they are used where contamination is less of a concern and can be more cost-effective.

Step 6: Assess Ergonomics and Ease of Use

Pipetting can be a repetitive task, and ergonomic design is crucial to reduce strain and improve user comfort. Consider:

Ease of Tip Attachment and Ejection: Tips should fit securely and eject easily without requiring excessive force.

Grip Design: Some tips come with a beveled or extended design that can improve grip and accuracy.

Step 7: Consider Bulk vs. Pre-Sterilized Packaging

Pipette tips are available in various packaging options, including bulk packs, racks, and pre-sterilized packs. Choose based on your workflow:

Bulk Packs: Cost-effective and suitable for high-throughput laboratories.

Racks: Convenient for everyday use, ensuring tips remain sterile and easy to access.

Pre-Sterilized Packs: Ideal for applications requiring strict sterility, such as cell culture or clinical diagnostics.

Step 8: Evaluate Cost and Budget

While it’s important to ensure quality and compatibility, budget considerations cannot be ignored. Assess the cost per tip and consider purchasing in bulk if it aligns with your usage and storage capabilities. However, avoid compromising on quality to save costs, as this can lead to inaccurate results and increased long-term expenses.

Step 9: Seek User Feedback and Reviews

Consult with colleagues or look for reviews and feedback from other users. Their experiences can provide valuable insights into the performance and reliability of different pipette tips.

Step 10: Conduct a Trial Run

If possible, obtain samples of different pipette tips and conduct a trial run in your laboratory. This allows you to evaluate firsthand how well the tips fit, their accuracy, and overall usability.

Selecting the right pipette tip is a crucial step in ensuring accurate and reliable results in the laboratory. By following this comprehensive guide, you can make informed decisions that align with your specific requirements, enhance your pipetting accuracy, and maintain the integrity of your experiments. Remember, the right pipette tip not only supports precision but also contributes to a smoother and more efficient laboratory workflow.

[liquidhandlingproduct]

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256cav dispolab tips mold

China medical mold maker, offer filter micropipette tips mold, disposable micro pipette tips mold, dispolab tips mold, plastic micropipettes mold, pipet tips mold

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Universal Pipette Tips In Laboratory Applications

The choice of pipette tips is a critical consideration that directly influences the accuracy, precision, and reliability of liquid handling processes. Among the various options available, Filter Pipette Tips and Universal Pipette Tips have gained prominence for their distinct advantages in addressing specific challenges associated with pipetting.

Filter Pipette Tips are equipped with a barrier, often made of hydrophobic material, situated at the end of the tip. This design offers several advantages, making Filter Pipette Tips particularly advantageous in applications where contamination prevention is paramount.

Aerosol Containment:

The primary benefit of Filter Pipette Tips lies in their ability to prevent the aerosolization of liquids during pipetting. The hydrophobic filter acts as a barrier, trapping any liquid that may be drawn into the pipette tip during aspiration. This is particularly crucial when working with biohazardous or volatile substances, minimizing the risk of sample contamination and exposure.

Reduced Cross-Contamination:

Filter Pipette Tips contribute to the aseptic technique by reducing the risk of cross-contamination between samples. The hydrophobic filter prevents liquids from reaching the pipette shaft, ensuring that only the intended sample is aspirated. This is essential in applications such as PCR, where even trace amounts of contamination can impact the reliability of results.

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Couldn't get the plastic off a new box of pipette tips so i just used the naked pipette to puncture a hole in the plastic