#Cell Culture

[consult-sherlockholmes]

Can I borrow ur skin for a min?

Which skin would you like? And for what kind of experiment are you intending to use it?

I have several different skins with different properties. In the fridge I have the skin of an arm on which I tested different corrosive qualities of acids to compare them. In the freezer there's an intact skin but it was obviously frozen so it won't have the same properties of fresh skin, but I needed to test the effect of freezing on human skin cells for a case where a body was kept in the freezer. I also have some petri dishes with normal human epidermal keratinocytes for another experiment. I could passage some of the cells into a new petri dish if you require those.

[bpod-bpod]

In the Round

Molecular and physical differences revealed between breast cancer cells lab-grown on a flat surface in 2D versus in 3D as spheroids – the nucleus of the 3D-grown cells is more rounded, has a larger surface, a more compact chromatin [DNA packaged with proteins in the nucleus] and a distinctive set of downregulated genes

Read the published research article here

Image from work by Julieta Ramirez Cuellar and Roberto Ferrari, and colleagues

Center for Genomic Regulation (CRG), Barcelona Institute for Science and Technology (BIST), Barcelona, Spain

Image originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)

Published in EMBO Journal, April 2024

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[miss-biophys]

Looking sexy in the cell culture lab

When I grow bacteria, I need to work extra clean not to infect myself. When I grow human cells (also cancer cells), I need to be even more careful not to infect the cultured cells with any bacteria from my clothes, skin, or hair. Otherwise they just die and we have no results of our experiments.

[aelloposchrysopterus]

A little comic I sketched today about learning to pipette, in a cross between lab journal entry and memoir.

[linstudy]

November 25, 2024 | pretty colors of cell culture

today i had to perform 2 different cell passaging. one with new and one with expired solutions. we have lots of expired medium, but they were well storaged. hopefully we can use them, it would be a shame to waste all of those expensive items. as far as i could see, this little devil, trypsin, works better than newly purchased one. lets hope mediums works as well as this one.

[himmelstudies]

16 August 2023 & 17 August 2023

I had sushi yesterday with my partner and got my favourite mentaiko sauce on the cooked items. 🍙 (My stomach cannot handle raw food sadly so no sashimi :c) Afterwhich, I went to listen to a research seminar and attended my lab session to check on my cells. There are growing pretty well I would say! 😄

My supervisor was really nice and has taught me a lot on how to handle the cells properly. I had to write up a manual for all the stuff he taught me for record purposes as well. But I do find it rather necessary as we are mainly conversing in our mother tongue, which I don't normally use when I am talking to anyone in the lab. So I am pretty glad there was this system in place to ensure I am understanding the information correctly! I didn't really do much after my lab ended when I got home as I am extremely exhausted and was supposed to have an early morning the next day but...

I slept past my alarm. By an hour. I had to rush to wash up and do my hair in 30 minutes without eating any food, except lozenges for my throat/cough. I managed to make it to my lecture hall kinda in time... I was late by 5 minutes but the professor was still doing the course introduction. So thank goodness for that. 🫣 Honestly glad to not be missing this class as I loved this module to bits. Second image was taken in my lecture hall. Since I was late, I felt bad to sit at the back, so I sat near the front of the professor. 🫥

After my lecture, I met with my partner again for lunch and headed to my school area for a short session to write up my lab manual and some key observations for my project so future me won't be dumb enough to do the steps incorrectly and risk re-culturing all the cells again. I will be heading to the lab again tomorrow after my lectures to do more lab training. 🦠 I am honestly kinda surprised that mammalian cells are quite easy to work with. (for now. This is my current impression. I feel like I might take these words back some time in future.) Hoping for tomorrow's session to be smoothsailing! ❤️💙

P.S. my old laptop died so I got a new laptop! Praying it will survive long! My old laptop lasted me 5 years before it died of multi-organ failure (AKA wifi card died, USB port all failed, keyboard malfunctioning, hinge for screen wobbly, trackpad having issues registering, audio port failure, and more... yep, I call it multi-organ failure 🥲)

[kosheeka]

Immortalized cell lines are highly regarded for scientific research. Over the period of a century, we have been able to collect numerous immortalized cell lines. They are either harvested from the tumors of individuals suffering from the condition. Unlike primary cells, which have a limited lifespan, immortalized cell lines can be generated by making genetic alterations that encourage continuous growth and division. This conversion is essential for providing a stable and sustainable source of biological material, which greatly benefits areas such as drug development, cancer studies, and genetic research. Having a deeper understanding of these procedures improves our capacity to effectively harness immortalized cell lines across various scientific disciplines.

Learn More: www.kosheeka.com

[theonetruekail]

Not something I’d normally post but I found these flower-shaped cell colonies at the bottom of a shotglass. Science tumblr can y’all help me figure out what they are?

[izadb]

[cherenkovs]

help why is this 2010 video from the eppendorf youtube channel making me scream...

[bpod-bpod]

Contractions and Wrinkles

Understanding the factors influencing myoblasts (cells from which skeletal muscle develops). In lab-grown cells, a soft substrate led to more myoblasts with a wrinkled nucleus with a reduced level of a proliferation marker, and lower contractility – a relationship with potential for therapeutic manipulation

Read the published research article here

Image from work by Jo Nguyen and colleagues

Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada

Image originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)

Published in Journal of Cell Science, March 2024

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[schaureninscience]

Daily cell culture life be like:

Astrocytes are so 🥺🤏🏼

[anthropologist-on-the-loose]

I'm as grateful for cellphones as the next person, but sometimes I think about how everyone having a phone on them at all times really did cause us to loose some things as a society. I mean - for example, kids these days will never experience their car breaking down and needing to find the nearest place with a phone they can use. They're never going to have the opportunity to tentatively approach a house only to discover that it's full of queer people having a party hosted by a transvestite to celebrate his creation of a sex homunculus, stay the night, and loose their virginity while unintentionally partaking in cannibalism. It's tragic, that kind of gay sexual awakening just doesn't happen these days because of cellphones.

[salengedusmiler-blog]

STOP IT THAT IS LITERALLY WHAT MY CELL CULTURES IN LAB ARE DOING EVERY GODDAMN DAY

if i was an animal and i knew i was being observed and researched i would do something super fucked up. but only once. never again. ruin their lives. keep them guessing.

[aelloposchrysopterus]

[linstudy]

December 17, 2024 | fried eggs mixed in my cell culture

ooh boy… where should I start? i f*cked up. yep. mycoplasma contamination is the worst. my only consolation is that these are my *practice* cells, not the real ones i should use for my thesis. i reported to my supervisor and waiting for a feedback. anyone has any advice?