#vaso6

[lupinepublishers]

Happy Thanksgiving Day Lupine Publishers | Assessment of Muscular Perfusion After Oral Intake of Pre-Workout Nitric-Oxide Stimulating Supplements by Contrast-Enhanced Ultrasound (Ceus).Study Protocol for A Placebo-Controlled, Randomized Cross-Over Trial in Healthy Young Males

Lupine Publishers |  Orthopedics and Sports Medicine

Abstract

Background: The market for dietary supplements in the sports sector has been growing rapidly for several years, though there is still lacking evidence regarding their claimed benefits. One group is that of nitric oxide increasing supplements, so-called “NOboosters”, which are claimed to improve the supply of oxygen and nutrients to the muscle by enhancing vasodilation.The aim of this study was to investigate three of these supplements in healthy male athletes for their muscle perfusion-enhancing potential using contrast-enhanced ultrasound (CEUS).

Methods: This placebo-controlled, double-blind, randomized cross-over trial will be carried out at the Center for Orthopedics, Trauma Surgery and Spinal Cord Injury of the University Hospital Heidelberg. Three commercial NO enhancing products including 300 mg of the specific green tea extract VASO6™ and a combination of 8 g L-citrulline malate and 3 g L-arginine hydrochloride will be examined for their potential to increase muscular perfusion in 30 male athletes between 18 and 40 years and will be compared with a placebo. On each of the three appointments CEUS of the dominant biceps muscle will be performed at rest and after standardized resistance training. Every athlete receives each of the three supplements once after a wash-out period of at least one week. Perfusion will be quantified via VueBox® quantification software. The results of CEUS perfusion measurements will be compared intra- and interindividually and correlated with clinical parameters.

Discussion: The results of this study may help to establish CEUS as a suitable imaging modality for the evaluation of potentially vasodilatory drugs in the field of sports. Other supplements could also be evaluated in this way to verify the content of their advertising claims.

Trial registration: German Clinical Trials Register (DRKS), ID: DRKS00016972, registered on 25.03.2019.

Keywords: Contrast-enhanced ultrasound; biceps muscle; muscle perfusion; L-citrulline malate; L-arginine; green tea extract; VASO6™

Background

Recently, the supply and the use of dietary supplements increase tremendously at all levels of athletic sports. Unfortunately, these much-courted supplements are often used without a full evidencebased understanding of the potential benefits and risks associated with their use.A vast number of supplements like proteins, vitamins, carbohydrates, or plant extracts are distributed promising several ergogenic effects. Especially supplements aimed at increasing the production of nitric oxide (NO) in blood vessels (so called NOboosters) make up one of the most popular supplement categories in the sport industry today.In endothelial cells, the enzyme isoform endothelial NO synthase (eNOS) catalyzes the conversion of L-arginine into L-citrulline and NO [1]. Among multiple effects on muscle physiology, NO acts as an endothelium‐derived relaxing factor with vasodilator properties while elevating intracellular cyclic guanosine monophosphate (cGMP) levels in smooth muscle cells [1-3]. Referring to this, products in this supplement category claim to improve the supply of oxygen and nutrients to the muscle via vasodilation and increased blood flow.

This supplement category includes, among others, L-citrulline, L-arginine and epicatechin gallates.

L-arginine is considered a semi-essential or conditional proteinogenic amino acid [4], which is endogenously synthesized mainly in the kidney from L-citrulline [5]. Furthermore, L-arginine is crucial for the normal function of the urea cycle, in which ammonia is detoxified and removed via metabolism into urea [6]. The effect of orally administered L-arginine on L-arginine plasma levels is limited, owing to its liver and particularly intestinal firstpass metabolism [5-8]. At the same time, oral intake of L-citrulline respectively its combination with L-arginine has been suggested to increase plasma L-arginine levels more efficiently than oral L-arginine alone, which was explained by inhibitory effects on catabolizing arginase enzymes as well as the fact that dietary L-citrulline is not metabolized by the liver and thus can serve as precursor of L-arginine in other tissues, e.g. the kidney [8-11]. Ultimately, this effect was associated with increased substrate availability for NOS [8]. Epicatechin gallates (ECG) are different components of botanical green tea or grape seed extracts. Botanical extracts contain numbers of phytochemicals which may provide health benefits. These phytochemicals can be isolated via fractionation [12]. Another product that is distributed in the category of NOboosting supplements is “VASO6™” (Serious Nutrition Solutions, 2965 Franklin Turnpike, and Danville, VA 24540, United States). This green tea extract is advertised as consisting of specific catechins which have attracted great interest as vasodilating agents ((product characteristics of VASO6™ [13], based on previous studies on procyanidins derived from extracts of grape seeds (GSE) [12, 14]. In 2002, single compounds of GSE fractions were bioassayed for endothelium-dependent relaxing (EDR) activity using the aortic ring model [14]. As isolated procyanidins showed most potential with NOS-mediated EDR activity increasing with the degree of polymerization, epicatechin content and with galloylation[14], specific procyanidin oligomers were proposed accordingly for the use in patients, especially the trimer-gallate epicatechin-(4–8)- epicatechin-(4–8)-epicatechin-gallate (C1-gallate) [15].

Previous studies have investigated the impact of these supplements advertised as “NO-boosters” on muscle blood flow or blood volume in resistance trained subjects with diverging results [16-19], making it challenging to draw a clear conclusion. Compared to the conventional examination tools that have been used in these studies such as strain-gauge plethysmography or Doppler-US, the increasingly applied technique of Contrast-Enhanced Ultrasound (CEUS) allows for a direct visualization of the microperfusion at higher resolution [20,21]. The contrast agent SonoVue®[24] which is commonly used for CEUS examinations, consists of microbubbles with a phospholipid shell and a sulfur hexafluoride core, an echogenic, poorly soluble gas [22]. Due to their size (comparable to that of red blood cells) [20],SonoVue® microbubbles remain intravascular after injection and oscillate measurably upon collision with ultrasonic waves, generating contrast-specific signals for (subsequent) quantification [21,23].SonoVue® is considered very safe with a low incidence of side effects [24]. The microbubbles are extracted from the lungs within 10 to 15 minutes by exhaling and the phospholipid shell is metabolized by the liver [21]. It does not interact with the kidneys. Thereby, it does not demand laboratory testing of renal function before administration and can be used in patients with renal dysfunction, when contrast agents for computed tomography (CT) or magnetic resonance imaging (MRI) are contraindicated.

Given the application of highly standardized examination algorithms, perfusion kinetics can thus be depicted in a reliable way [25]. The muscle perfusion quantified by CEUS allows insights into muscle (patho-)physiology that cannot be assessed by conventional MRI protocols [26]. In the past, CEUS has successfully been used to quantify skeletal muscle macro- and microperfusion and to assess the vitality of the skeletal muscle by means of the perfusion as a surrogate parameter in real time [26-29]. However, to our knowledge this method has not yet been used to investigate the impact of

pre-workout dietary supplements on muscular microperfusion. For this purpose, the following study will examine three different commonly used NO-boosting products via CEUS: -300 mg VASO6™ -8 g L-citrulline malate -3g L-arginine hydrochloride After oral intake, changes of muscular micro perfusion linked to resistance training will be quantified and the effects of supplementation will be compared with one another and with placebo in 30 healthy athletes.The study protocol for this study is described in the present manuscript.

Methods and Design

Go toObjectives

The primary objective is to generate hypotheses on the effects of selected supplement containing beverages on CEUS perfusion measurement in the resting and exercised biceps muscle compared to placebo. Therefore, the results of CEUS measurement will be compared intraindividually. In addition to that, the results of all participants will be sorted by beverage A, B and C and tested for significance.Moreover, several clinical parameters including body size, body weight and age will be correlated with the results of CEUS analysis.In future, CEUS could be an appropriate examination method to evaluate further potentially vasoactive supplements regarding their claimed impacts and benefits, especially in the sports sector. The current pilot study may thus contribute to initiate the implementation of CEUS in this field of research.Except for a compensation of 100 Euros, the participants have no self-interest or any personal advantages due to the study participation.

Study Design, Registration, and Ethics

The study protocol was approved by the local ethics committee (S-094/2019). Furthermore, it was registered at the German Clinical Trials Register (DRKS00016972). The study protocol will be conducted according to the Declaration of Helsinki.This is a registered, randomized placebo-controlled double-blind cross-over single-center trial.

Inclusion and Exclusion Criteria

Young male athletes aged between 18 and 40 years with periodical sporting activity in popular or serious sport are eligible for inclusion after giving their written consent. Exclusion criteria are recent myocardial infarction, cardiac or respiratory insufficiency, uncontrollable hypertension, severe respiratory disease (pulmonary arterial hypertension (PAH), severe acute respiratory syndrome (SARS), marcumarization or bleeding disorders, liver, intestine or kidney diseases and other known contraindications (e.g. allergies) to the contrast agent SonoVue®[24] as well as other similar contrast agents. Smokers in general will be also excluded. Additionally, persons who are not able to or do not agree to the informed consent will be excluded from the outset.

Enrolment of Participants and Study Setting

The present study is a randomized placebo-controlled doubleblind cross-over single-center trial.30 healthy male athletes are to be enrolled in this study. Only male athletes will be included because of better comparability. The recruitment is realized by a written announcement on a board in university buildings or in sports facilities. Further information can be requested by phone or email.Informed consent takes place in a face-to-face setting at the research site. A full verbal explanation of the study, a patient information sheet and informed consent form will be provided in advance. Each athlete will be informed in detail about the scientific purpose and risks of the study, its voluntary nature and the possibility to withdraw the agreement to participate in the trial at any time without giving any reasons. Athletes must be at least 18 years of age, without any exclusion criteria and provide their written agreement before any intervention. Moreover, the athlete’s health status will be assessed by a medical examination prior to the first examination.For every participant, the study includes three appointments for examination. In a randomized and doubleblind manner, the athlete receives one out of three beverages per examination (Figure 1) which always follows the same standardized procedure including CEUS perfusion measurement at rest and after exercising. The intervals between the study periods amount to at least seven days.

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Valentus Thermo Roast Dark Roast Coffee

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Valentus ThermoRoast Coffee Ingredients 

ThermoRoast Ingredients

Espresso Coffee

Caffeine

B-Phenylethylamine (PEA)

p-Synephrine HCL 

Green Tea Extract (as VASO6 tm)

(as Dynamine tm)

-Phenylalanine

Full Spectrum Cocoa Powder (5-HTP)

Chromium Polynicotinate

L-Theanine

  Espresso Coffee – Drinking espresso can be an appetite suppressant so you consume less calories. Studies show caffeine from espresso can also increase thermogenesis. Thermogenesis is an increase in your body temperature, which can also add to calorie burn.

An article in The American Journal of Clinical Nutrition showed 100 milligrams of caffeine could result in burning about 80 to 150 calories per day. One ounce of espresso has about 64 milligrams of caffeine.

That’s not a huge number of calories but it adds up over time. Burning 80 calories each day can lead to losing 8 pounds in a year.

p-Synephrine HCL – p‐synephrine is often used for athletic performance, suppressing appetite, weight control , increasing energy, and better focus.

Synephrine – is a stimulant. It has similarities with ephedrine and caffeine in it’s ability to burn calories and fat, add energy, and crush food cravings. It’s used to control weight and if taken along with caffeine the fat burning is kicked into high gear.

Dynamine (Methylliberine) – Dynamine is a trade name for a version of Methylliberine patented by Compound Solutions. Methylliberine is completely natural and it’s source is the kucha tree prmarliy the leaves of the tree. Similarities with caffeine and theacrine but with more positives and benefits.

Dynamine –Some will classify it as an ingredient for performance. An athlete’s or an active persons ultimate addition to their regimen. Adding Dynamine to Valentus ThermoRoast puts it on a higher level as a performance supplement.

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  DL-Phenylalanine (DLPA) – Phenylalanine is an ingredient naturally found in foods like milk, eggs, and meat that are rich in protein. An essential amino acid often offered as a diet supplement that has shown good results for pain, skin problems, and even anxiety and depression.

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Green Tea Extract (as VASO6 tm)  Another highly effective ThermoRoast component is Green Tea Extract. Known as an all-body reward system, Green Tea Extract benefits almost all organs in the body, including heart and brain health. It’s fights obesity, helps blood sugar control, protects the liver, and is good for the blood vessels.

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Full Spectrum Cocoa Powder (5-HTP) Cocoa Extract is another great ingredient in the Valentus ThermoRoast coffee product. It’s for heart health as well as stress and blood pressure. Mood improvements and depression relief can also be results of consuming 5-HTP.

L-Theanine Studies have shown a positive connection with L-Theanine and cognitive function and the lessening of cognitive loss. L-Theanine is also known for and regarded as an anti-anxiety supplement.

In addition L-Theanine assists with stress reduction and promotes calmness. A boost in attention spans and memory are also on the list of benefits from L-Theanine as well has promoting better sleep. Another great ingredient for ThermoRoast coffee.

Chromium Polynicotinate Chromium Polynicotinate is a trace mineral that has a number of highly beneficial functions that help with blood sugar balance and control. It can also help with sugar cravings and sugar withdrawal.

In addition to blood control, stable insulin levels and assisting insulin to perform better, Chromium Polynicotinate can also help to regulate cholesterol. That’s not all it’s also known to assist your work-outs by promoting the growth of lean muscle tissue.

  Alot of research has gone into the development of Valentus Thermo Roast coffee to improve it effectiveness and flavor we are sure you will love all the improvements 

   Learn More About 

Valentus Thermoroast Coffee

 Source-  https://hightech-health.com

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* These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose,treat, cure, or prevent any disease. Results not typical and may vary.

  from Valentus Products , Valentus Coffee , Valentus Emulin , Valentus Distributor https://valentus-products.com/valentus-products/valentus-thermo-roast-dark-roast-coffee/

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Valentus SlimROAST OPTIMUM Dark Roast Coffee with Dynamine

It's no secret that losing weight is no easy task, which is why Millions of people struggle with it every day. We want to make it a lot easier, that's why we created SlimROAST Optimum Dark Roast Coffee, which is designed to take your weight management to the next level!

Valentus Slimfast Optimum Coffee with Dynamine

SlimROAST Optimum Neurologic system for Optimum Performance cannot be accomplished with a single nutrient, it requires a "stack" (or group) of several independently performing nutrients working together. To deliver this stack to the right places in the brain, circulatory support nutrients that promote nitric oxide production and circulation (via vasodilation) is essential to optimize nutrient potential.

Formulated with natural appetite suppressants, feel good ingredients and detox components and powered by VASO6!22 a Nitric Oxide, Antioxidant Booster for Optimum Circulation & Oxygen delivery. SlimROAST Optimum is the perfect addition to your weight loss management program!

Not only will you find managing your weight with SlimROAST Optimum will produce exciting results, but you will love the taste of this delicious Dark Roast Coffee.

Directions & Supplement Facts

Directions

Mix 1 level scoop with 6-8 oz. hot water. Enjoy 1 or 2 cups per day.

Exclusive functional coffee formulated to:

13 Crush Cravings

13 Increase energy

13 Metabolize fat to energy

13 Help regulate Blood sugar

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Subscribe to our Compensation Valentus Disclaimer = I am an Independent Distributor For Valentus and make commissions on sales purchased through the links on these pages

Please Note this information is an explanation of Emulin Valentus Emulin Plus Products and is not to be used as medical advice, please always refer to your medical professional

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Valentus independent distributor

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We have tried many previous pre-workout drinks before and have always come to the conclusion that it could just be a placebo effect that gives you that extra boost to your workout.

Last month we were introduced to My Protein’s ‘The Pump’, the low-stimulant premium profile, THE Pump claims to be great in giving you the energy you need, so you can boost your workout. It also claims to be packed with clinically studied ingredients to get you closer to your workout goals.  

My Protein have rounded off the formula with super-popular ingredients as well such as AstraGin® and BioPerine®, sourced mainly from plants and being low in caffeine we couldn’t wait to put it to the test.

Mixing the powder in water half an hour before my workouts I even got to taste the Rainbow (no not the one on the advert), My Proteins ‘Rainbow Sherbet’ flavour powder and on first initial taste testing wasn’t displeasured in its taste and texture. 

One thing I did notice though was that my tongue tingled and felt warm towards the end of the drink, this being caused no doubt by the beta-alanine. 

For me, at first it was a bit unnerving as I thought I was having a reaction to the drink but then after realising this was the ingredient actually working I felt reassured that I was drinking something that was actually doing what it should be doing.

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I’m an early morning gym goer and to be honest I still don’t know if I felt that extra boost of energy from the powder or again from having a placebo affect but one thing I will say is it definitely made me feel more alert and focussed, which is always a good thing at 5am in the morning.

THE Pump™: Ingredient Breakdown

VASO6™: a patented ingredient, derived from green tea extract and containing naturally high levels of specific catechins and over 90% polyphenol content.

Vitamin C: helps your immune system so you can back to the gym time after time. Ideal for helping to support your natural defences after a tough workout.1

Iron: an important mineral that helps keep you sharp2 and supports circulation.3 We’ve finished off our blend with iron and vitamin B12 which helps to keep you on top of your game4 session after session.

Taurine: amino acids occur naturally in protein which helps to build and repair new muscle5 — that’s crucial whatever your fitness goals.

Thiamine: A notable ingredient in pre-workout blends, and helps support your cardiovascular system.6

1. Vitamin C contributes to maintain normal function of the immune system during and after intense physical exercise.

2. Iron contributes to normal cognitive function.

3. Iron Contributes to normal formation of red blood cells and haemoglobin and contributes to normal oxygen transport in the body.

4. Vitamin B12 contributes to the reduction of tiredness and fatigue.

5. Protein contributes to the growth and maintenance of muscle mass.

6. Thiamine contributes to the normal function of the heart.

https://www.myprotein.com/

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