#uv spectrophotometer

[lasanyinternational]

Exhibitions Certificate — Lasany International

Feeling proud and honored to receive this Certificate of Participation for our exhibit at Asia Labex and Asima and Global Lab. Our team put in so much effort and it's amazing to see it recognized. A big thank you to Lasany International for supporting us and believing in our work.

For More Information Call Us:- 9803047917

[labotronicsscientific]

Double-beam UV-Visible Spectrophotometer

Double-beam UV-Visible Spectrophotometer is adopted with microcomputer technology and electronic control system, features holographic grating with 1200 lines/mm. Equipped with solid silicone photodiode detector and xenon lamp as light source, have 190 to 1100 nm of wavelength range. Designed with 7-inch TFT screen with sensitive buttons and large sample chamber, it also supports USB storage and different data formats.

[microsilindia]

How to Choose the Right UV VIS Spectrophotometer in India

A UV-Vis spectrophotometer measures the absorbance and transmittance of light in the ultraviolet and visible regions of the electromagnetic spectrum. It is widely used in chemistry, biology, and materials science for analyzing the concentration of solutions, studying reaction kinetics, and characterizing compounds. Microsil India: Leading UV-Vis spectrophotometer manufacturer in India, delivering precision, reliability, and innovation for accurate scientific and industrial applications. The instrument provides vital data for qualitative and quantitative analysis in various research fields.

[kimberlabtron]

Atomic absorption spectrophotometer comes with Deuterium lamp for background correction, RS232 interface, Multi-linear and nonlinear curve fitting. Its double beam system automatically compensates the light source drift andw avelength drift caused by the variation of temperature and electronic circuit drift so as to reach a good basic line stability. The specially designed fine light beam passes through the flame to ensure a high precision analytical test and low characteristic concentration in this atomic absorption spectrophotometer. It has multiple applications in research and development and industrial analysis.

[crystalflores786]

Visible Spectrophotometer

A visible spectrophotometer is a laboratory instrument used to measure the intensity of light absorption or transmission of a sample in the visible region of the electromagnetic spectrum. It's a crucial tool in various scientific disciplines such as chemistry, biochemistry, biology, physics, and environmental science.

[ftirspectrophotometer]

Leading UV-Vis Spectrophotometer Xenon Lamp Manufacturer

Uncover brilliance in every wavelength with our Xenon Lamp-powered UV-Vis Spectrophotometers. Precision analysis for scientific excellence! We are leading UV-Vis Spectrophotometer Xenon Lamp Manufacturer, supplier and exporter from India. For product details or quotes contact us via email and chat support.

[labnic235]

UV-Visible Spectrophotometer

UV-Visible Spectrophotometer is a double beam instrument with a computer simulation optimized optical base. The continuously adjustable spectral bandwidth ranges from 0.1 to 5nm, making it ideal for samples with sharp absorption peaks. Precision is improved across the UV-Vis spectrum, from 190nm to 900nm, due to the well-aligned PMT detector and high-quality deuterium, tungsten, and mercury lamps. The device includes an optional 8-position cell holder as well as a high throughput 100-position autosampler with an easy-to-use interface.

[analabtron]

Single Beam UV/Vis Spectrophotometer

Wavelength range=190-to-1100-nm; Spectral bandwidth=2-nm; Wavelength Accuracy=plusmn-0-1nm-656-1-nm-plusmn-0-3-nm-all; Wavelength Repeatability=le-0-1-nm; Optical system=single-beam-1200-lines-mm. Shop Online at Labtron.us

[labozon]

UV-VIS Spectrophotometers

UV-VIS Spectrophotometers are Equipped with a microprocessor to set parameters and a 1200-line double beam grating, which ensures high resolution. For more, visit www.Labozon.com.

[vidoeslot]

Oh my god, I absolutely ADORE lab equipment. There's just something to dreamy about those little machines and it pains me so much that they're either expensive or impossible to get. My most recent crush has definitely been the Pharmacia LKB Ultrospec Plus UV-Vis Spectrophotometer, specifically one I saw in a tiktok that always gets the date wrong but they cost like 200 bucks and there are like 5 pictures of them online.

Sorry for the ramble, I'm just always happy to see fellow lab equipment enthusiasts <3

I LOVE THIS ASK, I absolutely adore lab equipment. there's a dearth of people talking about it, I should post some more... I've had the fortune to use some of the big biochem machines before and they are an absolute delight -- including the miniature sibling of that one I just reblogged, the NOVA FLEX2:

[electronicobjectum]

The niche attraction post is so real because I am attracted to the Pharmacia LKB Ultrospec Plus UV-Vis Spectrophotometer (I know, a mouthful) and it is such a specific model of such a specific object there are like 5 pictures of it online and they cost like 200 bucks😭

Anyway, if I ever get my hands on one, I'm gonna romance the hell out of it and until then, I'll continue to dreamily look at the same 3 listings of it

tumblr didn't notify me of this 🥹

looked it up and i definitely get where you're coming from, they're so shaped ☺️ i hope you get your hands on one someday!!

[lasanyinternational]

Visible Spectrophotometer Model – LI – 721s | Lasany International

Visible Spectrophotometer Model LI-721s by Lasany International is a precision instrument designed for accurate measurement of light absorbance in the visible spectrum. It features user-friendly controls and high reliability for various laboratory applications. This model ensures precise, consistent results suitable for research and quality control purposes.

[labotronicsscientific]

Double Beam UV-Visible Spectrophotometer

Double Beam UV-Visible Spectrophotometer is a compact, tabletop, double beam and Czerny-Turner monochromator comprised Double Beam UV-Visible Spectrophotometer, comes with 20 mm of focal length and 1600 lines/mm of grating. With 190 to 1100 nm of wavelength range, features adjustable 5-speed bandwidth of 0.5 nm, 1 nm, 2 nm, 4 nm, and 5 nm with fast-medium-slow scanning speed. Designed with Silicon Photocell detector, deuterium and tungsten lamp as light source, and 8-inch color touch-screen, supports spectra printing, storage and data analysis. Incorporated with USB communication port, ARM chip control and data processing, and easy user interface, this spectrophotometer has automatic scanning of measured spectrum, multi-wavelength (1-3 λ) measurement, kinetic measurement, 1-3 curve fitting, and 1-4 derivative spectra.

[helen0086]

Liposomes Coenzyme Q10 Quality Evaluation

Liposomes are ultra-microscopic spherical carriers that encapsulate drugs in a lipid bilayer. They exert their effect by being released through osmosis or phagocytosis by macrophages [1]. Liposomes have a variety of characteristics as drug carriers. They can encapsulate both water-soluble and fat-soluble drugs. In particular, they have attracted much attention for their ability to improve the selectivity of drugs for target organs, reduce toxicity and increase the therapeutic index.

Coenzyme Q1O (coenzyme Q1O) is a coenzyme-type biochemical drug [2, 3] that is a major component of proton transfer and electron transfer in the respiratory chain of cells of living organisms. It is an activator of cellular metabolism and respiration. Coenzyme Q1O can improve mitochondrial respiratory function, promote oxidative phosphorylation, protect the integrity of the structure of biological membranes, and has a significant anti-radiation effect and 5α-reductase inhibitory effect.

It is often used in clinical practice to assist in the treatment of coronary heart disease, hypertension, congestive heart failure and arrhythmia, and also has significant effects on photosensitivity, skin ulcers, dermatitis, bedsores, trauma and other conditions. In recent years, it has also been widely used in the treatment of hair loss, pigmentation and the elimination of wrinkles to improve the quality of life. Coenzyme Q1O liposome is a newly developed pharmaceutical preparation in this laboratory, and this experiment studied the quality evaluation method.

Materials and Methods

1. 1 Instrument and Reagents

Experiments: pHS-3TC precision digital pH meter (Shanghai Tianda Instrument Co., Ltd.); CARY-1OO UV-Visible spectrophotometer (Varian, USA); high performance liquid chromatograph (Waters, USA): 51O high pressure constant current pump, 486 variable square wave length data UV detector; SR2OOO chromatography data workstation (Shanghai Sunrui Technology Co., Ltd.); TSM ultrafine particle size analyzer (Shanghai University of Technology); FA 1OO4 electronic balance (Shanghai Tianping Instrument Factory); 8O-2 centrifugal sedimentation machine (Shanghai Surgical Instrument Factory). Coenzyme Q1O raw material (Nisshin, Japan), the reference substance is refined from the raw material, purity 99.98%; Coenzyme Q1O liposome (homemade); blank liposome (homemade); anhydrous ethanol (China Pharmaceutical Group Shanghai Chemical Reagent Company); water is deionized distilled water; the remaining reagents are analytically pure.

2 pH Value Inspection

According to the 2OOO edition of the Pharmacopoeia of the People's Republic of China, Appendix VI H, a glass electrode is used as the indicator electrode and an acidimeter is used for measurement.

1. 3 Morphological Observation and Particle Size Distribution Measurement

Coenzyme Q1O liposomes were taken, stained with a negative staining method, and the morphology of the particles was observed under a transmission electron microscope; the particle size distribution was determined using a TMS ultrafine particle size analyzer.

1.4 Determination of Coenzyme Q1O Content by RP-HPLC

1. 4. 1 Chromatographic Conditions

DABC18RPODS column (15Omm× 4. 6 mm , 1Oμm, Shanghai Yiqi Technology Co., Ltd.); mobile phase: anhydrous ethanol; flow rate: 1. Oml/min; ultraviolet detection wavelength: 275nm, sensitivity: 0. O5 AuFS, injection volume 1Oμl, external standard method for quantification.

1.4. 2 Standard Curve and Detection Limit

A series of coenzyme Q1O solutions were prepared with anhydrous ethanol to give mass concentrations of 1. O, 2. O, 4. O, 8. O, and 16. O ��g/ml, and detected by RP-HPLC.

1. 4. 3 Precision of the Method

Coenzyme Q1O standard solutions of 1. O, 2. O, 4. O, 8. O, and 16. O μg/ml were prepared, and the measurements were repeated 5 times within the day and between days.

4. 4 Sample Addition Recovery

Take an appropriate amount of empty liposomes, place them in a 1Oml volumetric flask, and measure precisely 25ml of coenzyme Q1O stock solution (O. 1g/ml) each. Shake well and determine the content according to the sample analysis method.

1. 4. 5 Sample Determination

Measure accurately an appropriate amount of coenzyme Q1O liposome, place it in a 1Oml volumetric flask, make up to the mark with absolute ethanol, shake well, and then dilute 1OO times with absolute ethanol. Shake well to obtain the test solution. Measure accurately 1Oμl of the test solution and inject it. Calculate the content of the test solution from the standard curve.

5 Determination of the Encapsulation Rate of Liposomes Coenzyme Q1

Dilute coenzyme Q1O liposomes 20 times with phosphate buffer (pH 7.4), measure an appropriate amount and place it in a centrifugal ultrafilter with a molecular weight cut-off of 5OOOO. Centrifuge at 3OOOr/min for 1O min to obtain a clear centrifugal liquid. Centrifuge the solution carefully, take an appropriate amount of the supernatant, dissolve it in anhydrous ethanol and make up to the mark. Shake well to obtain the test solution. Centrifuge a precise amount of 10 μl of the solution and calculate the free coenzyme Q10 content from the standard curve. Calculate the drug encapsulation rate according to the following formula:

where Wtotal and Wfree represent the total drug content and the free drug content in the liposome, respectively.

2 Results

1 Main Characteristics of Liposomes Coenzyme Q1O

Coenzyme Q1O liposomes are a pale yellow emulsion with a pH of 5. O~7. 5. They are mainly composed of unilamellar liposomes and a small amount of multilamellar liposomes. The particle sizes are relatively uniform and they are a colloidal colloidal dispersion; the particle size is basically normally distributed, with an average particle size of 0.184 μm and particles > 1 μm accounting for 0.17%.

2. 2 Results of RP-HPLC Analysis

2. 2. 1 Standard Curve Regression of Peak Area (A) Against Concentration (C) Gives the Standard Curve:

C= 6. 974× 1O— 5A—5. 894× 1O— 2, r = O. 9999(n=5)

The results show that the concentration in the range of 1. O~16. Oμg/ml has a good linear relationship with the peak area. The lowest detectable concentration of the method is 0. 1μg/ml, the lowest detection limit is 1ng, the retention time of coenzyme Q1O is about 4. 4min, and the theoretical plate number is more than 3OOO.

2. 2 Method Precision and Recovery

Intraday and interday RSD<2%. The average recovery rate was (1OO. 5±O. 65)%, n = 3.

2.2. 3 Sample Measurement Results

Three batches of samples were measured, and the coenzyme Q1O content in coenzyme Q1O liposomes was 94. 71%, 1O3.46% and 91. O9% of the labeled amount, respectively, all within the range of 9O. O%~11O. O% specified in the quality standard.

2.3 Encapsulation Rate of Liposomes Coenzyme Q10 

The encapsulation rate of three batches of coenzyme Q1O liposome samples was measured, and the encapsulation rate was >95%, which meets the requirements of liposome quality control.

3 Discussion

At present, the main methods used at home and abroad for ultrafine particle size analysis include optical microscopy, light transmission, laser scattering, and specific gravity methods. The TSM ultrafine particle size analyzer used in this experiment is a new type of particle size analyzer based on the principle of total light scattering [4]. It is used to measure the size distribution of ultrafine particles to meet the requirements of particle detection and size analysis.

A solution of coenzyme Q1O in absolute ethanol of a certain concentration was scanned with UV light in the wavelength range of 19O~ 35Onm. It was shown that coenzyme Q1O has a large absorption at 2O4, 275nm. Since there is interference in the measurement at 2O4nm, 275 nm was selected as the measurement wavelength in this experiment.

Literature reports [1] that there are many methods for determining the encapsulation rate, mainly including the dextran gel filtration method, ultracentrifugation method, dialysis method, etc. The dextran gel filtration method is a classic determination method, but it is cumbersome, time-consuming, and the raw materials are also more expensive; the ultracentrifugation method requires high instrument conditions and is expensive; the dialysis method requires continuous replacement of the dialysis solution. In this experiment, a centrifugal ultrafilter was used to determine the encapsulation rate. Coenzyme Q1O phosphate buffer can completely pass through the filter membrane. This method is convenient, fast, easy to operate, and has good reproducibility, making it more suitable for quality control in the production process.

Coenzyme Q10 is easily decomposed by light. Formulating it as a liposome preparation can improve the stability of the drug and prolong its duration of action. In addition, liposomes are hydrophilic carriers composed of phospholipid bilayers, which can also increase the compatibility of coenzyme Q10 with other hydrophilic components and increase its absorption in the body.

References:

[1] Gao Shen, ed. New dosage forms and technologies for modern drugs [M]. Beijing: People's Military Medical Publishing House, 2OO2. 197-228.

[2] Greenberg S, Frishman WH. Coenzyme Q1O: a new drug for cardiovascular disease [J]. J Clin Pharmacol, 199O, 3O(7) : 596- 6O8.

[3] Hoppe U, Bergemann j , Diembeck W , et al. Coenzyme Q1O, a cutaneous antioxidant and energizer[j]. Biofactors, 1999, 9(2- 4): 371-378.

[4] Cai XS. Measurement of particle size distribution with light extinction method [J]. Guangxue xuebao (Acta optica sin), 1991, 11(11): 49-53.

[tradesworthgroup]

Top Laboratory Equipment: Essential Tools for Accurate Scientific Research

In any scientific laboratory, having the right equipment is crucial for obtaining precise and reliable results. From basic instruments to advanced analytical systems, the tools in a lab help researchers perform experiments, analyze data, and reach conclusions. In this article, we explore the top laboratory equipment that plays a vital role in scientific research.

Spectrophotometers

Spectrophotometers are widely used for measuring the amount of light absorbed by a sample at different wavelengths. These instruments are vital in fields like chemistry, biology, and pharmaceuticals.

Key Uses:

Quantifying the concentration of DNA, RNA, and proteins.

Determining the purity of substances.

Types:

UV-Vis Spectrophotometer

Infrared (IR) Spectrophotometer

Chromatography Systems

Chromatography is essential for separating components of a mixture, and it includes a variety of systems suited to different types of samples.

Types:

Gas Chromatography (GC): Best for volatile compounds.

High-Performance Liquid Chromatography (HPLC): Ideal for separating liquid mixtures.

Thin-Layer Chromatography (TLC): For qualitative analysis.

Mass Spectrometers

Mass spectrometers measure the mass-to-charge ratio of ions and are crucial for identifying compounds, determining molecular structures, and quantifying substances.

Types:

Quadrupole Mass Spectrometer: Commonly used for quantitative analysis.

Time-of-Flight (TOF) Mass Spectrometer: Known for speed and resolution.

pH Meters

pH meters measure the acidity or alkalinity of a solution. Accurate pH measurement is vital for a range of industries, including water treatment, food, and pharmaceuticals.

Types:

Digital pH Meters: High precision for laboratory use.

Balances and Scales

Laboratories rely on balances and scales for accurate mass measurements. Analytical balances can measure down to micrograms, which is essential for preparing accurate solutions.

Types:

Microbalances: For ultra-precise weighing.

Top-Loading Balances: For general lab use.

Centrifuges

Centrifuges separate substances based on their density. They are used extensively in biological and biochemical research to prepare samples for further analysis.

Types:

Microcentrifuges: For small-volume samples.

High-Speed Ultracentrifuges: For high-density particle separation.

Thermocyclers (PCR Machines)

Polymerase Chain Reaction (PCR) machines are used to amplify DNA samples, making them crucial in genetics, diagnostics, and research.

Types:

Standard PCR Machines: For DNA amplification.

Real-Time PCR (qPCR): For quantifying DNA or RNA during the amplification process.

Atomic Absorption Spectrometers (AAS)

AAS is a technique used to measure the concentration of metal elements in a sample, including trace metals such as lead, mercury, and arsenic.

Applications:

Environmental testing

Food safety analysis

Fourier Transform Infrared Spectrometers (FTIR)

FTIR spectrometers are used to identify organic materials by measuring their absorption of infrared light.

Applications:

Pharmaceutical analysis

Quality control in material testing

X-Ray Fluorescence (XRF) Analyzers

XRF analyzers are non-destructive tools used to determine the elemental composition of materials, making them valuable in industries like mining and materials science.

Applications:

Geological surveys

Material analysis

Equipping a laboratory with the right tools is essential for conducting accurate research. Whether it's spectrophotometers, chromatography systems, or mass spectrometers, each instrument serves a specific purpose to ensure precise results. By investing in top laboratory equipment, researchers can maintain high standards of quality, ensuring their findings contribute to advancements in science and industry.

[crystalflores786]

UV-Visible Spectrophotometer

A UV-Visible spectrophotometer is a scientific instrument used to measure the absorption of light in the ultraviolet (UV) and visible (VIS) regions of the electromagnetic spectrum. It's widely used in various fields such as chemistry, biochemistry, molecular biology, environmental science, pharmaceuticals, and materials science.