#UV-Visible Spectrophotometer

[labnic235]

UV-Visible Spectrophotometer

UV-Visible Spectrophotometer is a double beam instrument with a computer simulation optimized optical base. The continuously adjustable spectral bandwidth ranges from 0.1 to 5nm, making it ideal for samples with sharp absorption peaks. Precision is improved across the UV-Vis spectrum, from 190nm to 900nm, due to the well-aligned PMT detector and high-quality deuterium, tungsten, and mercury lamps. The device includes an optional 8-position cell holder as well as a high throughput 100-position autosampler with an easy-to-use interface.

[labotronicsscientific]

Double-beam UV-Visible Spectrophotometer

Double-beam UV-Visible Spectrophotometer is adopted with microcomputer technology and electronic control system, features holographic grating with 1200 lines/mm. Equipped with solid silicone photodiode detector and xenon lamp as light source, have 190 to 1100 nm of wavelength range. Designed with 7-inch TFT screen with sensitive buttons and large sample chamber, it also supports USB storage and different data formats.

[lasanyinternational]

Best Blood Bag Tube Sealer Supplier And Manufacturer In India — Lasany international

One of our standout products is the Best Blood Bag Tube Sealer, which has been widely acclaimed for its reliability and durability. As a trusted blood bag tube sealer supplier, we offer advanced sealing solutions that maintain the integrity of blood samples, preventing any contamination or leaks.

Lasany International, we are not only dedicated to manufacturing top-notch medical equipment but also committed to being a preferred blood collection monitor supplier. We cater to various healthcare institutions, hospitals, clinics, and medical facilities across India and beyond. Our focus on customer satisfaction and prompt service has earned us the trust of countless clients nationwide.

Contact us today to learn more about our products and services and experience the Lasany International difference!

If you are looking for top-tier medical equipment manufacturers and reliable blood collection monitor suppliers in India, Lasany International should be your ultimate destination. Their commitment to excellence, product quality, and customer satisfaction make them the trusted partner in the healthcare industry.

[drpramod]

he UV visible spectrophotometer is one of the traditional Analytical techniques, which is widely used in the pharmaceutical industries for both quantitative(such as assay and content test) and qualitative analysis (such as identification test and to find out wavelength maxima of pharmaceuticals.

[microsilindia]

Exploring the Role of UV VIS Spectrophotometer in Modern Science

A UV Vis spectrophotometer measures the absorbance or transmittance of ultraviolet and visible light by a sample, providing insights into its composition and concentration. Commonly used in chemistry, biology, and material sciences, it enables precise analysis of molecules with chromophores. Applications include protein quantification, DNA analysis, and quality control in industries like pharmaceuticals and environmental monitoring. Microsil India, a trusted UV Vis spectrophotometer manufacturer, delivers precise, high-quality instruments for advanced research, industrial analysis, and academic applications, ensuring accuracy and reliability every time.

[helen0086]

Extraction and Immunoreactivity of Phycocyanin Subunits from Spirulina

Abstract:To investigate the extraction and immunoreactivity of alginate subunits of Spirulina alginata. Methods: The algal blue protein subunit was extracted by SephadexG-50 gel filtration after low-temperature wall-breaking and centrifugation to obtain the supernatant, which was filtered through a coarse fiber membrane, and the purity and molecular weight of the protein were detected by SDS-PAGE, and the absorbance at 620 nm and 280 nm was measured by infrared spectrometry and UV/visible spectrophotometry, so as to further observe the effect of the extract on the lymphocyte cells of the spleen of mice. The effect of the extract on mouse spleen lymphocytes was further investigated. Results: Extracts No. 1 and No. 2 were extracted by low-temperature aqueous extraction and gel filtration chromatography, and were dark green and blue water-soluble dry powders stable at room temperature, respectively. 1 and 2 extracts showed a protein band with molecular weight of about 17 kDa on SDS-PAGE.

Infrared spectroscopy showed that in the wavelength range of 1600~1800 cm-1 , one absorption peak was seen at 1656 cm-1 for Spirulina powder, three absorption peaks were seen at 1655 cm-1, 1633 cm-1 and 1594 cm-1 for extract No.1, two absorption peaks were seen at 1631 cm-1 and 1602 cm-1 for extract No.2, and one absorption peak was seen at 1658 cm-1 for the residue. Two peaks were seen at 1631 cm-1 and 1602 cm-1 for extract No.2, and one peak was seen at 1658 cm-1 for the residue. The UV/Vis spectrophotometer detected absorption peaks at 232.0 nm and 617.0 nm for extract No. 1, and 263.5 nm and 619.5 nm for extract No. 2. The purity of extract No. 1 was 0.78 with 15% A620/A280, and the purity of extract No. 2 was 0.85 with 20% A620/A280. The extracts were able to promote the proliferation of mouse spleen lymphocytes.

Conclusion:The subunits of algal blue protein were extracted at low temperature and possessed immune-enhancing activity.

Spirulina is a kind of filamentous multi-cellular spiral prokaryotic algae, rich in protein, fat, vitamins, minerals, chlorophyll, B-carotene and polysaccharides, which is an ideal food and medicine resource for human beings. Phycocyanin is an important active substance in Spirulina, in which phycocyanin, as a natural light-trapping pigment protein, only exists in a few algae such as cyanobacteria, and is a rare natural blue pigment, which has the physiological activities of enhancing immunity [1-2], antioxidant [3], anti-inflammatory and bacteriostatic [4], anticarcinogenic [5], preventing diabetes [6], and scavenging free radicals, and it can be made into biochemical medicines and used in food and nutrition as natural coloring and nutritional protein. It can be made into biochemical drugs, and can be used in food and cosmetic industry as natural pigment and nutritional protein. Using the strong fluorescence of algal blue protein, it can also be prepared into fluorescent probes and used in clinical testing. Phycocyanin consists of two subunits, α and β, and exists in the form of heterodimers such as (αβ)3 or (αβ)6 in the natural state. It has been found that the subunit has a small molecular size, good cell permeability and good anti-tumor activity[7] .

The extraction methods of algal cyanoproteins include ion exchange chromatography, gel filtration chromatography, ammonium sulfate gradient chromatography, hydroxyapatite, levano precipitation and expanded bed adsorption [8-10], etc. Different extraction methods have an impact on the large-scale production of algal cyanoproteins, the total yield, purity, protein activity, etc. The extraction of algal cyanoproteins is based on SepharoseG 75FF chromatography or SephadexG-75 gel column. Based on the extraction of cyanobacterial proteins, SepharoseG 75FF chromatography or SephadexG-75 gel columns were used to obtain cyanobacterial proteins, and the α-subunits were obtained by natural reduction of the β-subunits[11-12] . In the present study, we proposed to extract the crude extracts of α and β subunits of algal cyanobacteria by low-temperature wall-breaking, centrifugation to obtain the supernatant, and gel filtration and chromatography, and further investigate whether they are active or inactive, with the aim of providing a new way to utilize algal cyanobacteria as a high-value resource.

1 Materials and Methods

1.1 Materials

1.1.1 Reagents    

Spirulina powder was purchased from Fujian Fuqing Xin Da Ze Spirulina Co., Ltd; trypsin, Acrylamide, Tris-Cl, TEMED, Con A, MTT were purchased from Sigma; APS, SDS, bromophenolan, β-mercaptoethanol, glycerol were purchased from Shanghai Sangong; SephadexG-50 was purchased from Amersham.

1.1.2 Instruments   

GZX-9070MBE electric constant temperature blast drying oven was purchased from Shanghai Boxun; LGJ-18 freeze dryer was purchased from Beijing Songyuan Xinghua; DZG-303A ion water purifier was purchased from Moore; YDL5M centrifuge was purchased from Xiangyi Centrifuge Factory; TU-1901 UV/visible spectrophotometer was purchased from Beijing PUYA General Company; electrophoresis apparatus and electrophoresis baths were purchased from Beijing Liutyi Company; enzyme marker was purchased from thermo company. The electrophoresis instrument and the electrophoresis tank were purchased from Beijing Liuyi Company; the enzyme labeling instrument was purchased from Thermo Company, USA.

1.2 Methodology

1.2.1 Extraction and purification of algal blue protein subunits    

Dissolve 1 kg of spirulina powder in distilled water, put it in the refrigerator at -30~-35℃ overnight, thaw naturally and then crush and centrifuge it at 10 000 r/min for 10 min, separate the supernatant, retain the supernatant, remove the slag, and then extract the cyanobacterial protein subunits by SephadexG-50 gel filtration after filtration with a coarse fiber membrane and then obtain the dry powder by freeze-drying.

1.2.2 Spectral characterization    

The composition of the extracts was determined by infrared spectroscopy and scanned by UV/Vis spectrophotometer at the Analytical Center of Tsinghua University.

1.2.3 SDS-PAGE    

SDS-10% PAGE: Prepare 12% separating buffer and 4% concentration buffer, add samples, add equal volume of 2 × Loading buffer to the sample, boil at 100 ℃ for 5 min to denature, centrifuge at 10 000 r/min and centrifuge at 4 ℃ for 1 min, and then add the supernatant into the sample wells by pipetting with a spacer, and then electrophoreze at a constant pressure of 130 V. Bromophenol blue was used as an indicator until the electrophoresis reached the bottom of the gel. Electrophoresis was carried out, and bromophenol blue was used as an indicator until the electrophoresis reached the bottom of the gel. The gel was stained with Caulmers Blue R-250 for 30 min, then decolorized by shaking the bed until the bands were clear, and then photographed.

1.2.4 Purity and yield analysis   

The absorbance of the sample solution was measured at 620 nm and 280 nm by UV/Vis spectrophotometer, and the purity of the phycocyanin subunit was calculated according to the following formula.

Spirulina Extract Purity = A620/A280

Spirulina extract yield = alginin lyophilized powder mass/spiroplasma raw material mass × 100%.

Where:A280 and A620 are the absorbance at wavelengths of 280 nm and 620 nm, respectively.

1.2.5 Effects of extracts on mouse spleen mononuclear cells    

Mice were killed by decapitation, spleens were aseptically extracted, 200 mesh steel mesh grinding was used to make splenocyte suspension, 70% Percoll was used to isolate single nucleated cells, 2000 r/min for 20 min, cells were aspirated from the gray and white layers, IMDM washed for two times, and counted by 0.04% Taipolan staining, the cell viability was > 90%, and the concentration of the cells was adjusted to 5×106/mL for preparation. Negative control group, Con A positive control group, No.1 extract and No.2 extract experimental group were set up respectively. Negative control group did not add any stimulant, positive control group was stimulated by 5 μg/mL Con A, and the extracts were filtered and sterilized after configuration, and experimental groups were stimulated by 0.5 μg/mL, 1 μg/mL and 2 μg/mL extracts respectively. The experimental group was stimulated by 0.5 μg/mL, 1 μg/mL and 2 μg/mL of extracts respectively, and cultured in 96-well plates with three replicate wells, 5×105 splenocytes per well, and 200 μL of total system, and cultured for 44 h. After 4 h, MTT was mixed with the extracts, and 10 μL of MTT was added into each well.

2 Results

2.1 Characteristics and molecular weight of alginate subunit extract The protein was extracted from Spirulina powder by low temperature extraction and gel filtration chromatography, and two parts of extracts were obtained, which were called No.1 and No.2 extracts respectively. The results are shown in Fig. 1, respectively, for the original Spirulina powder (A), extract No. 1 (B), extract No. 2 (C), and the residue after extraction (D), the colors of which were in the order of dark green, dark green, blue and black, and the extracts were stored in the conventional room temperature.The results of SDS-PAGE are shown in Fig. 2, and the molecular weights of the protein of extract No. 1 and extract No. 2 were all about 17 kDa.

2.2 Spectral Scanning of Spirulina Extract    

The extract spectrum was scanned by infrared spectroscopy (Fig. 3), and the results showed that in the wavelength range of 1600~1800 cm-1, one absorption peak was visible at 1656 cm-1 for Spirulina powder, three absorption peaks were visible at 1655 cm-1, 1633 cm-1 and 1594 cm-1 for extract No.1, two absorption peaks were visible at 1631 and 1602 cm-1 for extract No.2, and one absorption peak was visible at 1658 cm-1 for residue. 2 peaks at 1631 and 1602 cm-1 and 1 peak at 1658 cm-1 for the residue. The above two extracts were taken separately, diluted to a suitable number of times, and scanned by UV/Vis spectrophotometer in the wavelength range of 200-800 nm. The results are shown in Fig. 4, No. 1 extract had absorption peaks at 232.0 nm and 617.0 nm, and No. 2 extract had absorption peaks at 263.5 nm and 619.5 nm, and the purity of No. 1 extract was 0.78 for A620/ A280, and that of No. 1 extract was 0.78 for A620/ A280, and that of No. 1 extract was 0.78 for A620/ A280. The purity of extract No.1 was 0.78 and the yield was 15%, while the purity of extract No.2 was 0.85 and the yield was 20%.

2.3 Extracts induced proliferation of mouse spleen lymphocytes    

In order to test whether the extracted proteins were active or not, the effect of the extracts on mouse spleen SINCs was observed. The stimulation results of different concentrations of the extract showed that the SI value of the positive control Con A group was 1.84±0.07, and the proliferation of mouse splenic mononuclear cells was obvious in all groups of the experimental group (P＜0.05 or P＜0.01). It is suggested that the extract of alginate subunit has the effect of promoting the proliferation of mouse splenic lymphocytes. See Table 1.

3 Discussion

Algal bile proteins are mainly found in cyanobacteria, red algae, cryptophytes and a few methanobacteria, and their main function is to act as light-trapping pigment complexes for photosynthesis. The known phycobilins can be divided into four main groups, namely phaeocyanin, phycocyanin, phycocyanin and allocyanin. Phycocyanin is one of the major algal bile proteins, and its extraction mainly involves wall-breaking and cell lysis, isolation, purification, drying, and product characterization [7]. Physical methods of wall breaking include ultrasonication, negative pressure cavitation, osmotic pressure impact, freeze-thaw, and chemical methods using acids, bases, detergents, enzymes, and so on. Different methods of destruction of Spirulina cell wall to different degrees, algal blue protein dissolution varies, research reports ultrasonic wall-breaking technology to deal with material wall-breaking rate is high, the purpose of the material extraction effect is good, and the algal blue protein extraction rate is high [10], but the protein extraction process, not only to extract the higher rate of protein, but also to maintain the activity of the protein, the literature reported that ultrasound is the propagation of mechanical vibration energy, in such an intense vibration sound field, in such an intense vibration sound field, in order to maintain the activity of the protein. Ultrasound is the propagation of mechanical vibrational energy, and in such an intense vibrational field, it can lead to changes in the function or structure of biological systems. Strong ultrasound irradiation of organisms can occur in the ultrasonic cavitation effect, so that the local temperature in the organism rises to thousands of degrees, which will cause changes in the structure of proteins and damage.

Considering the protein yield and the maintenance of protein activity, as well as the interference of chemical reagents, the protein was extracted by freeze-thawing method at low temperature in this study. There are several methods for further purification of protein extracts, such as ion exchange chromatography, gel filtration chromatography, ammonium sulfate gradient chromatography, hydroxyapatite, levano-precipitation, and expanded bed adsorption [8-10]. Ammonium sulfate chromatography coupled with hydrophobic chromatography was used to isolate and purify phycocyanin from Spirulina, and the high salt level may lead to denaturation of the protein. Hydroxyapatite separation is complicated and costly. The alginate obtained by levano-precipitation and expanded bed adsorption had a high overall yield, but the purity was low and further purification was required.Patil et al[13] devised a simple and efficient method consisting of a two-step procedure: dual-phase aqueous extraction and ion-exchange chromatography, but chemical reagents were also added to the procedure.14 The method is based on a combination of ammonium sulfate hydrolysis and hydrophobic chromatography.

In this study, we used the common separation method of centrifugal precipitation to remove the residue, and then used gel filtration chromatography to separate the different molecular weight proteins. Comparison of the infrared spectra of Spirulina powder, extract No.1, extract No.2 and residue showed that there were different degrees of differences in different wavelengths. The absorption peaks near the wavelength range of 1600-1800 cm-1 suggested the presence of proteins, and it was seen that there were differences between the absorption peaks of Spirulina powder and extracts in this range, and the peak areas in the range of the wave number of the extract were obviously larger than those in the range of the wave number of the extract, suggesting that the protein content was high. The peak area in the wave number range of the extract was significantly larger than that of the Spirulina powder, suggesting that the protein content was high. The molecular weights of the α- and β-subunits of phycocyanin were reported to be 18.4 kDa and 21.3 kDa, respectively [14], and the molecular weights were reported to be 15.4 kDa and 17.3 kDa, respectively [10], as well as 16 and 17 kDa, respectively [15], whereas extract No. 1 and extract No. 2 obtained in this study had only one band, and the molecular weights of these bands were both about 17 kDa, which is consistent with the literature [15]. This is consistent with the molecular weights of 16 kDa and 17 kDa for the α-subunit and β-subunit, respectively, as reported in the literature [15]. However, the SDS-PAGE results of Extract No. 1 and Extract No. 2 in this study showed a single band with the same molecular weight, which is inconsistent with the results reported in the literature [15] that there are two subunits of phycocyanin, and there are two bands on the SDS-PAGE results, so the results of the extracts of Phycocyanin No. 1 and Extract No. 2 in this study are not consistent with the results reported in the literature [15]. This is not consistent with the literature report[15] that there are two subunits of phycocyanin and two bands on SDS-PAGE.

The absorption spectra of the major algal bile proteins were reported to be different in the literature. Further scanning of the spectra of the extracts by UV/Vis spectrophotometer revealed that extract No. 1 had a maximum absorption peak at 617.0 nm and extract No. 2 had a maximum absorption peak at 619.5 nm, which was consistent with the literature report that phycocyanin had a maximum absorption peak at 615 nm-620 nm [ 7,16], and the literature reported [17] that the maximum absorption peak of visible phycocyanin was 620 nm and there was a clear shoulder peak at 600 nm. This is consistent with the literature report that algal blue protein has a maximum absorption peak at 615 nm-620 nm [7,16], and the literature report [17] shows that the maximum absorption peak of algal blue protein is 620 nm, and there is also an obvious shoulder peak at 600 nm, the maximum absorption peak of α-subunit is 624 nm, and the maximum absorption peak of β-subunit is 610 nm, and the peaks of the absorption peaks have a relationship with the extraction process of the protein, purity, and the PH value of the solution during the test, which is not an absolute value but is a fluctuation. This value is not absolute, but a fluctuation range, and the results suggest that extract No. 1 and extract No. 2 in this study are two subunits of algal blue protein respectively. The purity of phycocyanin was evaluated by the absorbance of A620/A280, and the purity of food grade was 0.7, the purity of reaction grade was 3.9, and the purity of the analytical grade was above 4.0.

In this study, the purity of extract No. 1 was 0.78 A620/A280 with 15% yield, and the purity of extract No. 2 was 0.85 A620/A280 with 20% yield. In the present study, food-grade algal blue protein subunits were extracted. Further investigation of its immunological activity revealed that the extract induced proliferation of mouse spleen lymphocytes. In conclusion, the extract was extracted by low-temperature aqueous extraction and gel filtration chromatography without the addition of any chemical reagents, and the extract was active. The group will further purify the extracts of algal cyanine subunits and explore the subunits and their bioactivities of extracts No. 1 and No. 2, so as to provide experimental data for the extraction and application of algal cyanine subunits.

References:

[1] Marín-Prida J, Pavón-Fuentes N, Llópiz-Arzuaga A, et al. Phyco⁃ cyanobilin promotes PC12 cell survival and modulates immune and inflammatory genes and oxidative stress markers in acute cere⁃ bral hypoperfusion in rats [J]. Toxicol Appl Pharmacol,2013,272(1): 49-60.

[2] Chang CJ, Yang YH, Liang YC, et al. A novel phycobiliprotein al⁃ leviates allergic airway inflammation by modulating immune re⁃ sponses [J]. Am J Respir Crit Care Med,2011, 183(1):11-25.

[3] Zheng J, Inoguchi T, Sasaki S. Phycocyanin and phycocyanobilinfrom Spirulina platensis protect against diabetic nephropathy by in⁃ hibiting oxidative stress [J].  Am J Physiol Inteqr Comp Physiol, 2013, 304(2): 110-120 .

[4] Pak W, Takayama F, Mine M, et al. Anti-oxidative and anti-in⁃ flammatory effects of Spirulina on rat model of non-alcoholic ste⁃ atohepatitis [J]. J Clin Biochem Nutr,2012,51(3):227 .

[5] Ravi M, Tentu S, Baskar G, et al. Molecular mechanism of an⁃ ti-cancer activity of phycocyanin in triple-negative breast cancer cells [J]. BMC Cancer,2015, 15(23):768-771.

[6] Ou Y, Lin L, Yang X, et al. Antidiabetic potential of phycocyanin: effects on KKAy mice [J]. Pharm Biol,2013,51(5): 539-544 .

[7] Kuddus M, Singh P, Thomas G, et al. Recent Developments in Pro⁃ duction and biotechnological applications of C-Phycocyanin [J]. Biomed Res Int,2013,8(26):1-9.

[8] Niu J F,Wang G C,Lin X, et al. Large-scale recovery of C-phyco⁃ cyanin from Spirulina platensis using expanded bed adsorption chromatography [J].  J Chromatogr B Analyt Technol Biomed Life Sci,2007,850(1-2):267-276 .

[9] Minkova KM, Tchernov AA, Tchorbadjieva MI, et al. Purification of C-phycocyanin from Spirulina (Arthospira) fusiformis [J]. J Bio⁃ technol,2003, 102(1): 55-59 .

[10] SHAO Mingfei, ZHAO Nan, LI Yongyong, et al. One-step column chromatographic purification of Spirulina alginata cyanobacterial protein [J]. Journal of Biology,2013, 30(5):59-63.

[11] GUO Rui-Yong, HUANG Bei, ZUO Man-Man, et al. Preparation of liposomes of phycocyanin subunits and their photodynamic antitumor effects [J]. Journal of Pharmacy,2008,43(10):1060-1065.

[12] TAN Yang, HUANG Bei, REN Yan Min, et al. Study on the cell permeability of phycocyanin subunits and their photosensitizing effect on tumor cells [J]. Laser Biology Letters,2007, 16(6):684-688.

[13]Patil G, Chethana S, Sridevi AS, et al. Method to obtain C-phyco⁃ cyanin of high purity [J]. J Chromatogr A,2006, 1127(1-2):76-81.

[14] Chen T, Wong YS, Zheng W. Purification and characterization of selenium-containing phycocyanin from selenium-enriched Spiruli⁃ na platensis [J]. . Phytochemistry,2006,67(22): 2424-2430 .

[15]Kumar D, Dhar DW, Pabbi S, et al. Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540) [J].  Ind J Plant Physiol,2014, 19(2):184-188.

[16] Lüder UH1, Knoetzel J, Wiencke C. Two forms of phycobilisomes in the Antarctic red macroalga Palmaria decipiens (Palmariales, Florideophyceae) [J J]. Physiol Plant,2001, 112(4):572-581.

[17] ZHANG Xin, LI Jianyong, GONG Xingguo. Separation of C-phycocyanin subunits and antitumor activity of Spirulina [J]. Journal of Zhejiang University(Science),2010,37(3):319-323.

#Phycocyanin #Spirulina #algalblueprotein #Phycocyaninextraction #Spirulinapowder

[emmarobinsonsworld]

Microplate Reader LMPR-B10

Labtron Microplate Reader is a UV-visible spectrophotometer with a UV-transmissive 96-well optical system. It features a long-lasting xenon lamp, wavelength range of 190-1000 nm and 0-15.0 OD measurement. With single, dual and full-wavelength detection, it supports quantitative/qualitative analysis and multiple calculation methods. Ideal for protein quantification and G protein detection.

[susanlabnic]

UV Visible Spectrophotometer 500 W

Labnic UV-Visible Spectrophotometer, which features a double beam optical system with a PMT detector, has a 190–900 nm wavelength range and 0.1–5 nm spectral bandwidth. Its ergonomic touch-screen, ultra-high-speed scanning, and compact design save 30% installation space.

[labotronicsscientific]

Double Beam UV-Visible Spectrophotometer

Double Beam UV-Visible Spectrophotometer is a compact, tabletop, double beam and Czerny-Turner monochromator comprised Double Beam UV-Visible Spectrophotometer, comes with 20 mm of focal length and 1600 lines/mm of grating. With 190 to 1100 nm of wavelength range, features adjustable 5-speed bandwidth of 0.5 nm, 1 nm, 2 nm, 4 nm, and 5 nm with fast-medium-slow scanning speed. Designed with Silicon Photocell detector, deuterium and tungsten lamp as light source, and 8-inch color touch-screen, supports spectra printing, storage and data analysis. Incorporated with USB communication port, ARM chip control and data processing, and easy user interface, this spectrophotometer has automatic scanning of measured spectrum, multi-wavelength (1-3 λ) measurement, kinetic measurement, 1-3 curve fitting, and 1-4 derivative spectra.

[lasanyinternational]

What are The Different Types of Equipment Used in Laboratories? Lasany International

Lasany International is the manufacturer of different types of equipment used in laboratories including microscopes, centrifuges, incubators, balances and scales, pipettes and syringes, spectrophotometers, autoclaves, freezers and refrigerators, and glassware and plastics.

Microscopes: Used to observe and magnify small objects, microscopes are essential in many scientific disciplines, including biology, chemistry, and physics.

Balances and Scales: These instruments are used to measure the mass, weight, and density of substances accurately.

Bunsen Burners: A standard tool used in chemistry labs for heating, sterilizing, and combustion reactions.

Glassware: Commonly used glassware in a lab includes beakers, flasks, test tubes, pipettes, and graduated cylinders.

Centrifuges: Used to separate substances based on their density, centrifuges are widely used in biological and chemical research.

Spectrophotometers: These instruments are used to measure light intensity in a sample and are commonly used in biochemistry and microbiology research.

Incubators: These instruments are used to create and maintain controlled environments for the growth of microorganisms, cells, or tissues.

Chromatography Equipment: This equipment separates compounds and analyzes their composition. Common chromatography instruments include gas chromatographs, high-performance liquid chromatography (HPLC), and thin-layer chromatography.

pH Meters: Used to measure the acidity or basicity of a solution.

Safety Equipment: This equipment includes gloves, goggles, lab coats, fire extinguishers, and other tools to ensure a safe laboratory environment.

For More Information Contact Us: 9803047917

[sudheervanguri]

Mankind Pharma, a renowned name in the pharmaceutical industry, is conducting a walk-in drive for experienced professionals in Quality Assurance and Quality Control. This drive is set to take place on September 1, 2024, from 9:00 AM to 5:00 PM at Hotel Silver Cloud, Ahmedabad. If you hold a degree in MSc Chemistry, B Pharmacy, or M Pharmacy and have relevant industry experience, this could be your chance to join one of India’s leading pharma companies. Job Location: RIICO Industrial Area, Kaladwas (Extn.), Udaipur, Rajasthan – API & Formulation Plant Available Positions and Qualifications 1. Quality Assurance (IPQA) Position: Sr Executive / Executive / Sr Officer / Officer Qualification: MSc (Chemistry), B Pharmacy, M Pharmacy Experience: 3 to 5 years Key Skills: Preparation of IPQA SOPs IPQA Checks, AQL, Line Clearance Review of BPR, Audit & Compliance Sampling of API & Drug Products Document & Data Control Control Sample Management 2. Quality Assurance (Qualifications) Position: Sr. Executive / Executive / Sr. Officer / Officer Qualification: MSc (Chemistry), B Pharmacy, BE (Instrumentation) Experience: 4 to 6 years Key Skills: Calibration & Qualification of Instruments/Equipment Utility/Water System/Environment Monitoring Process Validation, Cleaning Validation Technology Transfer for API & Drug Products 3. Quality Control Position: Sr Executive / Executive / Sr Officer / Officer Qualification: BSc, MSc (Chemistry) Experience: 2 to 6 years Key Skills: Analysis of RM, PM, Intermediates, APIs, Drug Products Proficiency in HPLC, GC, FTIR, UV-Visible Spectrophotometer Analytical Balance, Sampling [caption id="attachment_59502" align="aligncenter" width="930"] Mankind Walk-In Drive for Quality Assurance & Quality Control[/caption] Walk-In Interview Details Date: September 1, 2024 Time: 09:00 AM to 05:00 PM Venue: Hotel Silver Cloud, Opp. Gandhi Ashram & Dandi Bridge, Ashram Road, Wadaj, Ahmedabad - 380 027 Contact for Queries: Phone: 8955607876 Email: dhruv.jhala@mankindpharma.com Note: Immediate joiners will be given preference.

[qualityherb]

Development Trends of Chinese Plant Extract Manufacturers

Chinese Plant Extract Manufacturers can generally be divided into four tiers. The first tier includes A-share listed companies such as Layn Natural Ingredients and Chen Guang Biotechnology Group (CCGB), which are technologically advanced, have large-scale operations, and have high visibility. The second tier consists of some New Third Board-listed companies, such as Day Natural, and Red Star Pharmaceutical, which have a certain level of visibility and are continuously expanding in scale. The third tier includes companies like BGG WORLD and JOYWIN Natural Products, recognized by the industry, while the rest are relatively weaker players in the fourth tier.

Plant Extract Manufacturers typically feature the following main characteristics and equipment:

Extraction equipment: These devices are used to extract useful compounds from plant materials. Extraction processes can utilize various methods such as solvent extraction, supercritical fluid extraction, and distillation.

Separation and purification equipment: Extracted mixtures need to be separated and purified to obtain the desired compounds. This may involve techniques such as chromatography, crystallization, and membrane separation.

Testing and analysis equipment: Factories are typically equipped with devices for testing and analyzing to ensure product quality and purity. These devices may include high-performance liquid chromatography (HPLC), mass spectrometry (MS), and ultraviolet-visible spectrophotometers (UV-Vis).

Production and packaging equipment: Once compounds are extracted, separated, and purified, they are processed into final product forms and packaged for sale. This may involve processes such as formulation, drying, filling, and packaging.

Quality control and compliance: Plant extraction factories need to comply with relevant quality control standards and regulations to ensure product quality, safety, and compliance. This may include implementing good manufacturing practices (GMP), quality management systems (QMS), etc.

Major publicly listed companies in the Plant Extract Manufacturers:

Layn Natural Ingredients: Layn specializes in the research, production, and sales of plant extracts and natural drugs. Its flagship products may include various plant extracts used in pharmaceuticals, health products, cosmetics, etc. For example, they may provide active ingredients extracted from various plants for the manufacture of health products and medicines. Specific products may include grape seed extract, soy isoflavones, green tea extract, etc.

Chen Guang Biotechnology Group: CCGB is a high-tech enterprise specializing in the extraction, separation, purification, and application of effective plant components. Its main products include six major series with hundreds of products, among which natural pigments have the highest sales volume nationwide, capsanthin ranks first in the world in terms of sales volume, and capsicum oleoresin accounts for over 85% of domestic production. Lutein, lycopene, and other varieties are also important in the international market.

Possible future directions for Chinese Plant Extract Manufacturers:

Technological innovation and process improvement: With the continuous advancement of technology, plant extraction techniques and processes will continue to improve and innovate. Factories may invest more resources in the research and application of new extraction technologies to improve extraction efficiency, reduce costs, and develop more types of plant extracts.

Product diversification and functionality enhancement: With the increasing focus on health and beauty, the demand for plant extract products is expected to continue growing. Factories may increase efforts in the research and development of product diversification and functionality enhancement to develop more types of plant extract products with unique characteristics to meet the needs of different customer groups.

Quality control and compliance: With the intensification of market competition, factories may pay more attention to quality control and compliance. Strengthening quality management during the production process to ensure the safety, efficacy, and stability of products, and strictly comply with relevant laws and standards.

Sustainable development and environmental awareness: Against the backdrop of increasing environmental awareness, factories may pay more attention to sustainable development and environmental protection. Adopting more environmentally friendly production processes and materials to reduce energy consumption and waste emissions, and actively participating in green production and circular economy.

Expansion into international markets: With the advancement of globalization, Chinese plant extraction factories may increase their efforts to expand into international markets. Actively participating in international cooperation and exchanges, opening up overseas markets, and enhancing brand influence and competitiveness.

Chinese Plant Extract Manufacturer

[labnics25]

UV-Visible Spectrophotometer

Labnics UV-Visible Spectrophotometer is a compact, ergonomically designed instrument for optical measurements of transparent solutions or opaque solids. With a wavelength range of 190-1100nm and spectral bandwidth of 2.0/4.0nm, it performs analyses like Photometric T%, Absorbance, Standard Curve, and Time Scan Kinetics. Features include low noise, low stray light, real-time data storage, durable construction with a reinforced baseboard, and an LCD display with a numeric keypad.

[microsilindia]

How to Choose the Right UV VIS Spectrophotometer in India

A UV-Vis spectrophotometer measures the absorbance and transmittance of light in the ultraviolet and visible regions of the electromagnetic spectrum. It is widely used in chemistry, biology, and materials science for analyzing the concentration of solutions, studying reaction kinetics, and characterizing compounds. Microsil India: Leading UV-Vis spectrophotometer manufacturer in India, delivering precision, reliability, and innovation for accurate scientific and industrial applications. The instrument provides vital data for qualitative and quantitative analysis in various research fields.