#uhlc

[leedsomics]

Urine-HILIC: Automated sample preparation for bottom-up urinary proteome profiling in clinical proteomics

Urine provides a diverse source of information related to health status and is ideal for clinical proteomics due to its ease of collection. To date, there is no standard operating procedure for reproducible and robust urine sample preparation for mass spectrometry-based clinical proteomics. To this end, a novel workflow was developed based on an on-bead protein capture, clean up, and digestion without the requirement for processing steps such as precipitation or centrifugation. The workflow was applied to an acute kidney injury (AKI) pilot study. Urine from clinical samples and a pooled sample were subjected to automated sample preparation in a KingFisher Flex magnetic handling station using a novel urine-HILIC (uHLC) approach based on MagReSyn(R) HILIC microspheres. For benchmarking, the pooled sample was also prepared using a published protocol based on an on-membrane (OM) protein capture and digestion workflow. Peptides were analysed by LCMS in data independent acquisition (DIA) mode using a Dionex Ultimate 3000 UPLC coupled to a Sciex 5600 mass spectrometer. Data was searched in Spectronaut 17. Both workflows showed similar peptide and protein identifications in the pooled sample. The uHLC workflow was easier to set up and complete, having less hands-on time than the OM method, with fewer manual processing steps. Lower peptide and protein CV was observed in the uHLC technical replicates. Following statistical analysis, candidate protein markers were filtered, at [≥] 2-fold change in abundance, [≥] 2 unique peptides and [≤] 1% false discovery rate, and revealed many significant, differentially abundant kidney injury-associated urinary proteins. The pilot data derived using this novel workflow provides information on the urinary proteome of patients with AKI. Further exploration in a larger cohort using this novel high-throughput method is warranted. http://dlvr.it/SsvYGd

[urbanhandmadecollection]

The #UHLC unisex backpack was handcrafted with 100% high-quality leather in order to be your best, long-lasting friend as the leather is thick and very durable to time and conditions. It has a very spacious main compartment and five other pockets for you to carry your gadgets such as: 15" laptop, cellphone, iPad, scarf, camera, books, water bottle, pen/pencil, cosmetics, magazine and many many more • • • • • #UrbanHandmadeCollection #UrbanHandmadeLeatherCollection #Leather #LeatherCraft #HandCrafted #Instagram #InstaGood #Love #Wax #Ankara #AfricanPrint #Kente #Mudcloth #Kitenge #AfricanFashion #Tourism #Entrepreneur #WomenEntrepreneurs #Backpack #Unisex #TravelBag #WorkBag #LeatherBag #GadgetBag #Africa #WestAfrica #MadeInGambia #MIG #🇬🇲 (at The Gambia) https://www.instagram.com/p/BtrPh1FHIFe/?utm_source=ig_tumblr_share&igshid=20pscv7h5gai

[universityofhouston]

LEGALLY PROUD. UH Law Center ranked among top five most affordable law schools with the highest starting salaries for graduates in the nation. bit.ly/2rsB4mE

[amazingarchitecturewebsite]

10³ Boutique Hotel in Maoyang Town, #China by LQS Architects Read more: Link in bio! Photography: Lu Qishui, Ma Qing The project is located in Maoyang Town on the coast of Ningbo City, Zhejiang Province. The plot is a typical homestead with its three sides surrounded by mountains and one side faced with the sea. The site is very close to a scenic spot, so the client wished to build it into a hotel and expected to receive high profits. With modern design methods, LQS Architects made this white cubic building stand out uniquely among its surroundings. It has become a landmark in that area and has gain popularity among young people… #hotel #pool #архитектура www.amazingarchitecture.com ✔ A collection of the best contemporary architecture to inspire you. #design #architecture #amazingarchitecture #architect #arquitectura #luxury #realestate #life #cute #architettura #interiordesign #photooftheday #love #travel #construction #furniture #instagood #fashion #beautiful #archilovers #home #house ‎#amazing #picoftheday #architecturephotography ‎#معماری (at China) https://www.instagram.com/p/Cif2er-uhLc/?igshid=NGJjMDIxMWI=

[beholdersgazedice]

Spring Blossom 💜 Available at www.beholdersgaze.com #springblossom #dice #beholdersgaze #smallbusiness #eyegax #happyrolling #diceporn #diceset #polyhedraldice #geeklife #gamerlife #dicegame #gameofdice #diceofinstagram #dungeonsanddragons #dnd #dnddice #ttrpg #tabletopgames #rpg #rpgdice #criticalrole #pathfinder #dungeonmaster https://www.instagram.com/p/B-iEH-UhlCS/?igshid=3qbla157gomp

[lettherebemonsters]

Ehhh... Hey loser uwu

I see a UHLC coming! An unidentified Hey Loser comment!

Lol thank you so much! X3

[sista0509]

@Regran_ed from @urbanhandmadecollection - Have you shopped our backpacks yet? The toughest decision is which one to buy 😉 We had a pleasure seeing the faces of our little ones light up with excitement when they got their #UHLC bags for school reopening. #UHLC2018Collection • • • • • #UrbanHandmadeCollection #UrbanHandmadeLeatherCollection #Leather #HandmadeLeather #Handcrafted #Tradition #LeatherCraft #Handcrafted #Bespoke #InstaGood #Love #Ankara #AfricanPrint #Kente #Creativity #Kids #BackPack #BunnyBackpack #BackToSchool #Urban #WomenEntrepreneurs #Tourism #ArtsAndCrafts #Gambia #Africa #WestAfrica #MadeInTheGambia #🇬🇲 https://www.instagram.com/p/BorUDI7AXPh/?utm_source=ig_tumblr_share&igshid=8l0i5ce7qmq

[todaynewsguru]

DOST Admissions: TSCHE announces schedule for special drive; check dates

DOST Admissions: TSCHE announces schedule for special drive; check dates

According to the official schedule, the registration window will close on October 28. The window for web options will open on October 26 and close on October 28. The verification of certificates at UHLCs will take place on October 28. Press Note for Special Drive Phase release on 22.10.2022 pic.twitter.com/4gO7fcOMBI — DOST-Degree Online Services, Telangana (India) (@dost_telangana) October 22,…

[blairemclaren]

Jon David ("JD") Epstein Death - Jon David ("JD") Epstein Has Died

Jon David (“JD”) Epstein Death – Jon David (“JD”) Epstein Has Died

Jon David (“JD”) Epstein Death – Obituary, Cause Of Death – Former UHLC adjunct professor of health law Jon David (“JD”) Epstein has passed away. His obituary appeared in the Houston Chronicle. Our heartfelt condolence to everyone mourning this death. Click link to read more from source Former UHLC adjunct professor of health law Jon David (“JD”) Epstein has passed away. His obituary appeared…

[marcelakane]

International Association of Women Honors Marcela Kane as a 2018-2019 Influencer of the Year

[thecbdstoreri]

To all my #friends who own small #business’s. Our thoughts and #prayers go out to each of you who have been negatively impacted by the #COVID-19 virus. With all the #brightest #minds in the #world working on a solution, I am hopeful there will be a #cure, #vaccine or #drug developed soon. While the #virus is disrupting many businesses, it’s also providing new #opportunities for the business #owners looking for them. I have repeatedly seen throughout my #entrepreneurial career that with each #challenge comes #new #opportunities. While most #entrepreneurs throw their hands up in #defeat when faced with significant #challenges, you won’t read a #biography of a #successful #person who #quit when times got tough. Remember, #quitters never #win and #winners #never #quit. (at The CBD Store of Warwick) https://www.instagram.com/p/B-Uhlc-H-v7/?igshid=4rzldeboau18

[urbanhandmadecollection]

We made these beautiful cowrie sunglasses for our #UrbanPrincesses & #UrbanPrinces Your little ones will love them • Available @ the trade fair Stall: #174 #FekamaTradeFair • • • • #UrbanHandmadeCollection #UHLC #UrbanHandmadeLeatherCollection #Handmade #Leather #HandmadeLeather #UrbanDecorHomewares #MadeToOrder #CustomMade #WellMade #LeatherCraft #Handwoven #Handcrafted #Bespoke #BespokeBag #BespokeBags #Wax #Ankara #AfricanPrint #KenteCloth #Cowrie #Sunglasses #Africa #African #WestAfrica #Gambia 🇬🇲 (at Independence Stadium (Bakau)) https://www.instagram.com/p/CPbco6sNylk/?utm_medium=tumblr

[universityofhouston]

LEGALLY PROUD. UH Law Cougars traveled to Columbia for a moot court competition, conducted entirely in Spanish. bit.ly/2q8TiwL 

[humansofjohnjay]

José Luis Morín is a Professor and Chairperson of the Latin American and Latina/o Studies (LLS) Department.  Professor Morín is also Director of the Rossana Rosado Fellows Program, which provides unique internship opportunities to students in the LLS major and minor.  He is also an Advisory Board Member to the John Jay College Ronald H. Brown Law School Prep Program and the UHLC Pre-Law Pipeline Program, two programs that the LLS department runs in collaboration with St. John's University School of Law and the University of Houston Law Center to increase access to and diversify the legal profession. 

Watch the full video spotlight of Prof. Morín: https://youtu.be/QecBL4iG_Is

[mektext]

UHLC observes Black History Month with a lecture on race and the law by noted legal scholar and author, Paul Butler

UHLC observes Black History Month with a lecture on race and the law by noted legal scholar and author, Paul Butler

UHLC observes Black History Month with a lecture on race and the law by noted legal scholar and author, Paul Butler

via PR Newswire: African American

[sista0509]

@Regran_ed from @urbanhandmadecollection - The ultimate #UHLC wax/ankara baby carriers are designed for safe and easy babywearing, simplifying day-to-day tasks, travels and getting around for parents. Using an infant carrier can help you better bond with your baby & keep them close without missing out on life’s big and small moments. All carry positions: Forward facing & back They support newborns to toddlers from 3-13 kg #UHLC2018Collection #ProductNumber3 • • • • • #UrbanHandmadeCollection #UrbanHandmadeLeatherCollection #Leather #HandmadeLeather #Handcrafted #Tradition #LeatherCraft #Handcrafted #Bespoke #InstaGood #Love #Ankara #AfricanPrint #Kente #Creativity #Kids #BabyCarrier #Toddlers #Babies #Urban #WomenEntrepreneurs #Tourism #ArtsAndCrafts #Gambia #Africa #WestAfrica #MadeInTheGambia #🇬🇲 https://www.instagram.com/p/BoboiZjAztK/?utm_source=ig_tumblr_share&igshid=18ajcgrfoq8tg