#senolytic activator

[life-longevity-goals22]

Embracing the Future: Life Extension Senolytic Activator

In the grand mosaic of existence, there's a thread that binds us all: the pursuit of longevity. From the enchanting tales of the Fountain of Youth in ancient lore to the cutting-edge advancements of modern science, humanity has always sought to extend the boundaries of life. Now, standing at the threshold of a new era, we have a chance to redefine what it means to age with grace, vitality, and purpose. Enter Life Extension Senolytic Activator – a beacon of hope in the quest for extended youthfulness and healthspan.

At its core, Life Extension Senolytic Activator targets senescence – the biological process that drives aging. As we journey through life, our bodies accumulate senescent cells, akin to retired soldiers no longer contributing positively to the cause. These cells not only deteriorate our physical well-being but also pave the way for age-related ailments, casting shadows over our golden years.

But here's where the magic happens: Life Extension Senolytic Activator steps in to clear the clutter, sweeping away those senescent cells and paving the path for rejuvenation. Imagine it as a cosmic broom, sweeping aside the cobwebs of aging to reveal the brilliance beneath. By doing so, this supplement doesn't just mask the symptoms of aging; it addresses the root cause, offering a holistic approach to reclaiming youthfulness from within.

Think about waking up each morning feeling as though time has rewound, your body humming with vitality and vigor. Tasks that once seemed daunting now feel like child's play, and the world brims with possibilities once more. With Life Extension Senolytic Activator, age truly becomes just a number, while the essence of youth reignites within.

Yet, the impact of Life Extension Senolytic Activator extends far beyond the individual. By promoting healthier aging, it has the potential to alleviate the strain of age-related diseases on healthcare systems globally. It offers a lifeline to millions, promising a future where the twilight years are not marked by frailty but by fulfillment.

As we stand at this crossroads of history, the choice is ours to make. Will we resign ourselves to the inevitability of aging, or will we grasp the reins of destiny and steer towards a future where age is but a gentle whisper in the wind? Life Extension Senolytic Activator beckons us to embrace the latter – to embark on a journey where the sands of time flow ever gently, and the promise of youthfulness knows no bounds. So, join us as we step boldly into the future, where the fountain of youth awaits those brave enough to seek it.

[jim-fetter-illustrations]

We Humans aren't designed to live forever, but we can live longer than we do!

Most “anti-aging” supplements that many popular websites and books recommend do NOT slow down your aging process, they mostly benefit your health as you get older, and you are still ageing at the same rate you always have.

These are substances like vitamin A, vitamin E, coenzyme Q10, lipoic acid, B-vitamins, calcium, omega-3 fatty acids, zinc, multivitamins, curcumin, EGCG, nicotinamide riboside (NR), mushroom extracts and many more are great supplements but they won't slow down your aging!

This means many touted “anti-aging” supplements are nothing more than antioxidants, which do improve your health as you age, like vitamin A, vitamin E, coenzyme Q10, lipoic acid and so on. But they don’t slow down aging. Some antioxidants can even accelerate aging, like lipoic acid or vitamin A and vitamin E.

Ya see,... We human beings aren't supposed to live forever so we have an aging process which triggers (kicks in) at a certain age, and it's downhill from there on. We have a built in fail point, just like automobiles do, so they can keep selling us cars forever.

Ya wanna know what does slow down your ageing process, and they SLOW DOWN not stop it..........

1. "Fisetin", a natural ingredient found in vegetables and fruits, especially in strawberries. Fisetin is mostly known for its senolytic activity, meaning it can clear away senescent cells, ..... zombie cells literally that have done their job biologically but reuse to die off, and are still hanging around sponging off of other healthy cells, like many of our political leaders do.

2. Alpha-ketoglutarate (the calcium form)

Alpha-ketoglutarate (AKG) is a substance that naturally occurs in our bodies. When we get older, the levels of AKG decline. Most studies show in mice that they live 14% longer than mice that didn't take Alpha-ketoglutarate (AKG).

3. Microdosed lithium

Lithium is a mineral found in nature. It seeps from rocks into water, including drinking water, like natural spring water.

Various studies show the more amount of lithium in the drinking water the longer people have been living in that area, and had less neurodegenerative diseases like Alzheimer’s.

So the further you are from the city the better off health wise you are.

Can you see the stars at night,...... if not your still to close to the city!

4. Glycine

Glycine is an amino acid that occurs naturally in our body. When we age, glycine levels decline.

Glycine has many functions in the body. It improves the epigenome (the machinery that determines which genes are switched on or off, a process that goes increasingly awry when we get older). Glycine especially improves the epigenome of mitochondria, the power plants of our cells.

5. Pterostilbene

Pterostilbene is the better brother of the famous anti-aging substance resveratrol.

Resveratrol has long been hyped as a longevity substance. However, it unfortunately did not live up to that hype. Studies showed disappointing results when it came to resveratrol extending lifespan.

6. Malate or malic acid

Malate, also called malic acid, is found in apples, and in our own bodies.

Malate is an important substance in the mitochondria. In fact, malate is a component of the Krebs cycle, which consists of various substances that are chemically modified to provide the energy that keeps all cells going.

7. Magnesium

Magnesium is an indispensable mineral for the body to function properly.

Magnesium helps innumerable enzymes in our body to function properly. Cells shuttle magnesium in and out to propagate nerve signals and to generate muscle impulses, including the beatings of our heart.

Magnesium also sticks to our DNA, stabilizing our DNA, protecting it against damage. Increasing DNA damage is one of the reasons why we get older.

8. Glucosamine (the sulfate form)

Most people know glucosamine as a substance to reduce wear and tear of cartilage and to improve joint health.

Few people know that glucosamine can also extend lifespan in different organisms, including mice.

Studies show that glucosamine is one of the few supplements associated with reduced mortality in humans, and also reduced risk of cardiovascular disease in humans.

9. Hyaluronic acid

Hyaluronic acid is an important component of the skin. But hyaluronic acid (HA) surrounds and embeds many other cells in the body than just the skin cells.

The older we get, the less hyaluronic acid there is in the body. A 70-year-old has only about 19 percent of the amount of hyaluronic acid of a young person.

Studies show that hyaluronic acid, taken orally, can improve skin appearance by reducing wrinkles, improving moisturization of the skin and increasing skin radiance. It can also improve osteoarthritis, which makes sense given joints and cartilage contain a lot of hyaluronic acid.

10. Ginger

Ginger is a well-known spice. But it’s not just any spice, it's THE SPICE for longevity.

Many scientific studies demonstrated multiple beneficial health effects of ginger, like reducing inflammation and protecting cells against damage.

Ginger has been found to extend lifespan in simple organisms, like fruit flies.

Ginger can improve type 2 diabetes and inflammation in humans.

11. Rhodiola rosea

Rhodiola rosea is a very interesting plant that grows in the northern regions of Europa and Asia.

Rhodiola rosea has been used for centuries as an adaptogen, a substance that can improve resilience against both physiological stress and mental stress.

Rhodiola can also improve nerve regeneration.

Studies in humans show that Rhodiola rosea can improve memory, concentration and can reduce fatigue.

12. L-theanine

Theanine is a substance found in green tea, and is one of the reasons why green tea is healthy.

Theanine has been shown to extend lifespan in simple organisms.

Theanine has been associated with healthier blood vessels, and could reduce blood pressure and even obesity.

Theanine has shown to reduce neurodegeneration and protect neurons in the body.

13. Nicotinamide mononucleotide (NMN)

Nicotinamide mononucleotide (NMN) is needed to make NAD+.

NAD+ is a very important substance in the cells. It provides energy for cells and is also a cofactor for proteins that repair and maintain our epigenome and our DNA.

NMN improves metabolism and reduces inflammation.

In conclusion I can say I've studied anti-aging for decades and many anti-aging supplements are based on outdated insights and don’t have any or just very little science backing up their claims.

Also, they do not contain substances that have been shown to act on aging mechanisms.

I personally have been taking 5 of these 13 supplements for a decade, so I should outlive your grandchildren, Lol

I would say if you just took one of these 13 it should be L-Glycine, because Glycine is like the switch that turns on the light in the room, and if the switch doesn't work nothing else gets what it need to illuminate you.

Ginger being a close second to take, which both are fairly cheap in cost to get pharmaceutical grade supplements, and remember only pharmaceutical grade supplements are worth a damn, because Choosing a pharmaceutical-grade supplement is the only way to know for sure that you're giving your body the highest, purest, and most bioavailable form of an ingredient possible, so if it doesn't say "pharmaceutical-grade" on the label, it isn't!!

As Spock from Star Trek would say "Live Long And Prosper", and you do that by knowing how!

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[jhavelikes]

The accumulation of senescent cells is associated with aging, inflammation and cellular dysfunction. Senolytic drugs can alleviate age-related comorbidities by selectively killing senescent cells. Here we screened 2,352 compounds for senolytic activity in a model of etoposide-induced senescence and trained graph neural networks to predict the senolytic activities of >800,000 molecules. Our approach enriched for structurally diverse compounds with senolytic activity; of these, three drug-like compounds selectively target senescent cells across different senescence models, with more favorable medicinal chemistry properties than, and selectivity comparable to, those of a known senolytic, ABT-737. Molecular docking simulations of compound binding to several senolytic protein targets, combined with time-resolved fluorescence energy transfer experiments, indicate that these compounds act in part by inhibiting Bcl-2, a regulator of cellular apoptosis. We tested one compound, BRD-K56819078, in aged mice and found that it significantly decreased senescent cell burden and mRNA expression of senescence-associated genes in the kidneys. Our findings underscore the promise of leveraging deep learning to discover senotherapeutics.

Discovering small-molecule senolytics with deep neural networks | Nature Aging

[verofax]

Reversing the Clock? Could Dietary Supplements Hold the Key to Extended Lifespan?

The pursuit of longevity has long captivated humanity. Now, groundbreaking research from Dr. David Sinclair at MIT offers a potential paradigm shift: a method to potentially extend human healthspan through cellular rejuvenation. This isn’t science fiction – Dr. Sinclair’s approach focuses on dietary supplements aimed at preventing the buildup of “aging cells,” which contribute to age-related diseases.

Understanding the Science: Targeting Senescent Cells

As we age, our bodies accumulate senescent cells, damaged cells that stop dividing but don’t die. These “zombie cells” secrete harmful substances that disrupt surrounding healthy cells, accelerating aging and contributing to age-related diseases like heart disease, cancer, and Alzheimer’s.

Dr. Sinclair’s research focuses on targeting these senescent cells. His approach utilizes dietary supplements designed to:

Clear Senescent Cells: These supplements might encourage the body’s natural mechanisms to eliminate senescent cells more efficiently.

Prevent Senescence: Supplements could potentially interfere with the processes that cause cells to become senescent in the first place.

The Promise of Dietary Supplements: A Healthier, Longer Life?

If successful, Dr. Sinclair’s approach could revolutionize healthcare. Imagine a future where age-related diseases become less prevalent, and people enjoy a longer period of health and vitality. Here are some potential benefits:

Reduced Disease Burden: By eliminating senescent cells and their harmful effects, the risk of age-related diseases could be significantly reduced.

Extended Healthspan: A longer healthspan translates to more years spent living an active and fulfilling life.

Improved Quality of Life: With a reduction in age-related ailments, individuals could potentially enjoy a higher quality of life well into their later years.

A Note of Caution: Early Days and Important Considerations

While Dr. Sinclair’s research is exciting, it’s crucial to maintain a balanced perspective:

Early Stage Research: Dr. Sinclair’s work is still in its early stages. Extensive clinical trials and safety assessments are needed before these supplements can be widely recommended.

Focus on Healthy Habits: Supplements are not a magic bullet. Maintaining a healthy lifestyle with proper diet, exercise, and sleep remains paramount for healthy aging.

Regulation and Accessibility: As this research progresses, regulations and accessibility considerations regarding these supplements will need to be addressed.

The Future of Aging – A Multifaceted Approach

Dr. Sinclair’s research on senolytics offers a fascinating glimpse into the future of aging. While dietary supplements hold promise, it’s likely that a multifaceted approach will be needed to achieve significant longevity breakthroughs. This may include advancements in other areas like regenerative medicine, gene editing, and personalized healthcare.

Staying Updated on the Longevity Frontier

Dr. Sinclair’s breakthrough research is a significant step towards a future where aging is not a disease but a manageable condition. As the field of longevity research continues to evolve, staying informed about ongoing developments will be key. Keep an eye on this space for further updates on clinical trials, regulatory developments, and the potential impact of Dr. Sinclair’s work on the future of aging.

For more insightful tips and expert advice, follow me on LinkedIn, Facebook, Instagram, Twitter, TikTok, Threads, YouTube channel and stay tuned!

For more information, visit Verofax or contact info@verofax.com.

Blog source: https://verofax.com/blog/reversing-the-clock-could-dietary-supplements-hold-the-key-to-extended-lifespan

[sudheervanguri]

CSIR-Central Drug Research Institute Hiring Project Associate Are you an M.Pharm or MSc graduate in Biochemistry or Zoology looking for a rewarding career in research? CSIR-Central Drug Research Institute (CDRI) is currently hiring Project Associates. Located in Lucknow, this prestigious institute offers an exciting opportunity to work on cutting-edge projects with a team of dedicated professionals. If you have expertise in cell culture, animal handling, and molecular biology, this could be the perfect role for you. CSIR-Central Drug Research Institute Hiring Project Associate - M.Pharm, MSc About the Job Department: Research & Development (R&D) – Active Pharmaceutical Ingredients (API) Position: Project Associate Vacancies: 01 Project Code Number: GAP0475 Project Title: Novel ẞ3-adrenergic agonists as senolytics and insulin sensitizers: Studies in adipose tissue of metabolic dyshomeostasis model systems Scheduled Completion: May 2027 Location: CSIR-Central Drug Research Institute, Lucknow Responsibilities Conduct research and development activities for API projects. Perform synthesis and characterization of organic compounds. Collaborate with cross-functional teams to ensure project success. Document experimental procedures and results accurately. Ensure compliance with regulatory and safety guidelines. Qualifications Degree: M.Pharm with GPAT qualification or MSc in Biochemistry/Zoology with NET/GATE qualification. Experience: Freshers and professionals with relevant experience. Skills: Expertise in cell culture, animal handling (diabetes and obesity models - mice, rat, hamster), histopathological studies, cell and molecular biology. Salary and Benefits Monthly Emoluments: ₹31,000 + 16% HRA Upper Age Limit: 35 years (age relaxation as per Government of India norms) Application Process Interested candidates can apply by filling the Google form provided here. The last date for submitting the application is 18/07/2024, up to 5 PM. Selection Process Screening: The duly constituted Screening Committee will shortlist candidates based on their qualifications and experience. Interview: Shortlisted candidates will be notified via email and will attend the interview (online/offline) as communicated. Important Information The positions are purely temporary, initially for one year, and extendable based on performance and project duration. Engagement in these projects does not confer any right to regularization against any CSIR/CDRI post(s). The number of positions may vary at the time of selection depending on the project requirements. Contact Information For further queries, please contact: Email: info@csir.cdri.in Address: CSIR-Central Drug Research Institute, Lucknow [caption id="attachment_86214" align="aligncenter" width="1200"] CSIR-Central Drug Research Institute Hiring Project Associate[/caption] Instructions To apply for the above post, please go through the Instructions given below and fill the Google Sheet (link given above) All applicants must fulfill the essential qualification for the position and other conditions stipulated in the advertisement as on the last date of submission of application. They are advised to satisfy themselves before applying that they possess at least the essential qualifications laid down for position/positions as on the last date of submission of application. The prescribed essential qualifications are the minimum and the mere possession of the same does not entitle candidates to be called for interview. The duly constituted Screening Committee will adopt its own criteria for short-listing the candidates. The candidate should therefore, mention in the application all the qualifications and experiences in the relevant area over and above the minimum prescribed qualification, supported with. List of shortlisted candidates meeting the criteria (Essential qualification plus criteria set by the committee) shall be notified on the CDRI Website. Date and mode of interview shall be communicated to the shortlisted candidates through e-mail.

The position/s are purely temporary. The engagements shall be initially for a period of ONE YEAR or till the project duration or to the extent funds available, whichever is earlier. The engagements shall be extendable on yearly basis subject to performance review and project duration / funds availability. The engagement of a project staff in different projects either in the CSIR - Central Drug Research Institute, Lucknow or different Lab./Institute of CSIR taken together shall not exceed 5 years in any case. The engagement in these projects will not confer any right implicit or explicit on the candidate for consideration for regularization against any CSIR/CDRI post(s). The number of positions to be recruited may vary at the time of selection depending on the requirement under the project. No TA/DA or allowances will be paid to the candidates for attending the interview. Suitable Candidates may be empaneled for future requirement if any, under same project or different projects of similar nature and requiring candidates with similar essential and desirable qualifications. Age will be reckoned as on the last date of submission of application for this advertisement. Age relaxation will be provided as per Government of India norms. Candidates seeking age relaxation should enclose relevant certificates in prescribed format issued by the appropriate authority as per the latest instructions issued on the subject. Applications (filled in google sheet) submitted after the scheduled date and time shall not be considered for the interview and summarily rejected. The shortlisted candidates shall attend the interview (online/offline), as communicated to the candidate. In case a large number of candidates apply, the Screening Committee may include the additional screening criteria beyond essential and desirable qualifications and shortlist the candidates for interview in proportion to the available vacancies. to interact in English/Hindi before the Selection Committee. Candidate has the option 16) In case the final certificates reflect CGPA/SGPA/OGPA Grades, the candidate should convert the same into an equivalent percentage as per the approved formula of the University in the Google Form, and a copy of such conversion must be attached along with the google form. To avoid any inconvenience, only those candidates who strictly fulfil the eligibility criteria and possess the degree's / mark sheets in the subjects above should apply for the interview. Upon selection, the original documents, including eligibility qualification, DOB, caste, and age relaxation certificate, will be verified. If any discrepancy is found, the selection shall stand cancelled automatically. Advertisement / post may be cancelled at any stage due to force majeure, including closure of project, non-availability of fund, compromised integrity, unethical practices, etc. The decision of the CSIR-CDRI in all matters relating to eligibility, acceptance or rejection of applications, mode of selection, and conduct of examination/interview will be final and binding on the candidates.

[scientificinquirer-blog]

DAILY DOSE: Study Explores Atrial Fibrilation And Elite Athletes; Senolytics Target Zombie Cells in Clinical Trials.

TOUR DE FRANCE CYCLISTS FUEL HEART HEALTH STUDY. On July 1, 2023, as the Tour de France kicked off from Bilbao to the Pyrenees, sports cardiologist Andre La Gerche observed the event keenly from Melbourne. He is particularly interested because some of the leading cyclists are part of his study on the impact of endurance exercise on heart health. The research explores how such activities could…

[knowledgeandinformation]

How not to age

Slowing pathways of ageing

Only about 15-30% of our lifespan appears determined by our genes, which means how we live our lives may determine the bulk of our destiny.

AMPK

Autophagy is a housekeeping process by which defective cellular components are broken down and scrapped for spare parts. AMPK induces autophagy. The most reliable way to extend lifespan may be long term food reduction. AMPK activation is thought to be one of the mechanisms for this longevity boost. Exercise increases the AMPK activity. To help boost AMPK a) reduce consumption of saturated fat (concentrated in meat, dairy and desserts)b) increase consumption of fibre, c) 2 teaspoons of barberries, d) a dash of black cumin, e) hibiscus and/or lemon verbena tea, f) 2 teaspoons of vinegar

Autophagy

To boost this anti-aging pathway, consider a) 60 minutes of moderate to vigorous aerobic activity, b) minimise your intake of fried food, c) consume at least 20mg of spermidine by incorporating foods such as tempeh, mushrooms, peas and wheat germ into your diet, Wheat germ has 2.5mg per tbsp, mushrooms 9.2mg per 100g, tempeh 9.7mg per 100g d) drink three cups of regular or decaffeinated coffee.

Spermidine plays a key role in regulating cell growth. Wheat germ can significantly improve dementia way beyond all available antidimentia treatments so far.

Cellular senescence

To prevent cellular senescence, avert DNA damage by following the recommendations in the oxidation chapter and to clear such cells of their SASP there are natural senolytic compounds in foods (quercetin, fisetin and piperlongumine). While it is not yet clear whether sufficien levels can be reached by eating foods rich in these compounds, the foods are healthful in their own right. To slow this aging pathway, consider a) consuming quercetin-rich foods, beverages and seasonings such as onions, apples, kale, tea and salt free capers, b) eating strawberries, c) seaoning meals with pippali.

Epigenetics

Exercise frequency and intensity are associated with a deceleration of aging. In 2018 an aging analysis was published of the CALERIE study, the first major randomised trial of calorie restriction in humans. The control group continued to age at a rate of about one year per year but in that time the dietary restriction group only aged by about one month. And they achieved this with only a 12% calorie restriction. Aging rates were slowed independent of weight loss.

The lifestyle factor most closely associating with slowing aging, even more than exercise, is a marker of fruit and vegetable intake, blood levels of carotenoid phytonutrients like beta-carotene. The food most consistently linked to accelerating aging is meat.

To help boost this anti-aging pathway, restrict calories by 12% which would be cutting about 250 calories out of a 2000 calorie diet, and b) meeting the 400μg recommended daily allowance of folate, which could be achieved with about a cup of cooked lentils or edamame, a cup and a half of cooked spinach or asparagus, or two and a half cups of broccoli.

Glycation

Advanced glycation end products (AGEs) are one of the main factors contributing to the aging process. As AGEs accumulate in our bones, joints and muscles, they may contribute to osteoporosis, arthritis and muscle asting, the weaking, shrinking and loss of muscle mass with age. AGEs are implicated in age related memory decline, impaired wound healing, skin aging, cataracts and Alzheimer's disease.

Eating an AGE less diet by emphasising lower AGE foods such as fruits and vegetables, cooking high protein foods using relatively low heat and high humidity methods such as boiling or steaming rather than broiling or frying, favouring raw nuts and seeds over roasted or toasted, choosing lower glycemic load foods. Steel cut oats are a low glycemic index food but instant oats are high.

IGF-1

The higher the IGF-1 in your bloodstream, the higher your risk for developing some cancers such as breast, colorectal and prostate.

Inflammation

Chronic inflammation is systemic, persistent and non-specific and appears to perpetuate disease. A marker is C-reactive protein. Immunity deteriorates with age in a process known as immunosenescence. There is also more inflammation. The progressive increase in pro-inflammatory status is a major feature of the aging process and is referred to as "inflammaging". Applying skin lotion twice daily may be a simple way to dampen systemic inflammation. Some causes of inflammation are the buildup of dietary advanced glycation end products, senescent cells spewing SASP and age related decline in autophagy machinery. Two years of modest caloric restriction cut inflammation markers such as CRP by 40%, which could have been due to a boost in autophagy that cleared out inflammatory cellular debris or simply as a consequence of their weight loss. Fatty tissue plays an active role in secreting inflammatory chemicals. Dietary cholesterol may contribute to inflammation. As we age, there is an increate in visceral fat which may contribute to inflammaging.

Dietary Inflammatory Index: generally components of animal products and processed foods like saturated fat, trans fat and cholesterol were found to be pro-inflammatory, while constituents of whole plant foods such as fiber and phytonutrients came up strongly anti-inflammatory. Eating a more inflammatory diet has been associated with 75% increased odds of having cancer and 67% increased risk of dying from it.

mTor

Suppression of mTOR may be a central mediator of the lifespan-extending effects of dietary restriction. Caloric restriction has been heralded as a fountain of youth but negative side effects may include dangerously low blood pressure, infertility, slower healing of wounds, menstrual irregularities, sensitivity to cold and loss of strength, bone and libido. You're also walking around starving all the time. However the benefits of eating less may not be coming from restricting calories but from restricting protein. Methionine and the three branch-chain amino acids (BCAA) isoleucine, leucine and valine are particularly important to restrict, and they are particularly concentrated in animal proteins. A compound called DIM, formed when the cruciferous vegetable compound indole-3-carbinol hits our stomach acid, has been shown to suppress mTOR activation. Sulforaphane, another product of consuming cruciferous vegetables, also suppresses mTOR. Also coffee and green tea. To slow this aging pathway, strive to stick to the recommended daily intake of protein, 0.8g per healthy kg of body weight which translates to about 45g a day for the average-height woman.

Oxidation

Free radicals are unstable, violently reactive molecules with an unpaired electron. The unpaired electron steals electrons away from any molecule in their path. The resultant cellular damage causes aging. Mitochondria are the major source of cellular free radical formation. You can reduce mitochondrial free radical production rate through exercising and by lowering intake of the amino acid methionine. Methionine restriction is thought to account for about 50% of the lifespan extension attributed to full dietary restriction. Legumes have a comparably low methionine content. This is consistent with legume consumption being the most important dietary predictor of survival in older people around the world. Antioxidants can't seem to slow the rate of aging but they may be able to prevent age-related diseases linked to oxidative damage to the 99% of our DNA outside the mitochondria. Oxidative stress has been implicated in hair graying, the development of cataracts, arthritis, frailty and neurodegenerative, cardiovascular, kidney and pulmonary diseases, cognitive decline, age-related macular degeneration and muscle loss. Every meal is an opportunity to tip the balance in a pro-oxidant or antioxidant direction. Eating a single meal deficient in antioxidant-rich foods can leave us in a pro-oxidant state for hours. You can also experience increased oxidative stress due to illness, secondhand smoke, air pollution and sleep deprivation. Antioxidant levels can plummet within 2 hours of a stressful event and take up to 3 days to get back to normal. So healthier eating is especially important when we anticipate we'll be stressed, sick or tired. Kiwis, cooked carrots and green tea are particularly good at facilitating DNA repair. Red wine can improve blood antioxidant capacity. Nrf2 levels and signaling tend to decrease with age. 30 mins of cycling can boost them but the most potent natural Nrf2 inducer is sulfurophane (in cruciferous vegetables). B12 deficiency is associated with increased oxidative stress. To slow oxidation, consider daily exercising, restrict methionine intake by choosing plant based protein sources and reducing overall protein intake to recommended levels, activate Nrf2 defenses by eating green/ cruciferous vegetables and drinking green tea, eating berries and other naturally vibrantly coloured foods, using herbs and spices, avoiding added salt, sugar and saturated fat- and cholesterol-rich foods.

Telomeres

Telomeres are one of the aging pathways that have crept into the public consciousness. Increasing telomere length to slow or even prevent aging is a popular idea, though the science is controversial. Telomere elongation is possible through activation of the telomerase enzyme, but there is a constant battle between the forces hacking away at our telomeres such as aging, oxidative stress and inflammation and the lifestyle decisions that can help build them back up. To help boost this anti-aging pathway, on a daily basis consider: a) following the recommendations in the inflammation and oxidation chapters, b) eating a high-fibre diet centered around whole plant foods, drink tea and coffee, eat cruciferous vegetables, supplement with 800 to 1000 IU of vitamin D3 a day if your vitamind D blood level is under 20 ng/mL.

Lifestyle

Weight

BMI of 20 to 22 is the optimal weight for longevity (53-58kg for 5ft4).

Sleep

Sleep deprivation is no joke. The magnitude of impairment from a week of five-hour nights is similar to that reported in people who smoke, have diabetes or have coronary artery disease. There are some alarming contaminants in melatonin supplements. Pistachios are not just the most melatonin-rich nut, they are simply off-the-charts as the most melatonin-rich food ever recorded. To get a physiological dose of melatonin, all you have to eat is two pistachios.

Salt

Between 40 and 70 food intake drops by about a quarter due to a declining appetite. We also start losing our taste buds and sweet and salty tastes are often the first to slip, which can lead to diets particularly excessive in sugar and salt.

Preserving function

Bones

Inflammation and oxidative stress may play a role in osteoporosis. The intake of pro-inflammatory foods and an elevation of inflammatory markers in the blood such as C-reactive protein are both associated with osteoporotic fractures. A higher intake of fruits and vegetables is associated with lower fracture risk. The consumption of vitamin-C rich foods is associated with lower risk of bone loss, osteoporosis and hip fracture. Soy foods have been significantly associated with lower risk of fracture.

Nutritionally, soy milk is the best choice for replacing dairy milk in the human diet.

Bowel

Fibre.

Circulation

The capacity of our blood vessels to repair themselves is dependent on endothelial progenitor cells that emerge from stem cells in our bone marrow to patch up any holes in our endothelium, the innermost lining in our blood vessels that keeps our blood flowing smoothly. Reduce trans fat (mostly in meat and dairy), saturated fat (animal products and junk foods. also coconut oil palm oil) and dietary cholesterol (animal products, particularly eggs). Increase berries, onions, green tea. Eat plant based.

Immune system

Vaccination is less effective for the elderly. For example, while flu shots can build up sufficient antibody protection in 50-70% of younger individuals, that proportion falls to as few as 10-30% of older adults who are among those who need the protection the most. Also, the immune cells of elderly produce significantly more pro-inflammatory signals. This suggests the worst of both worlds - a decline in the part of the immune system that fights specific infections and an aggravation of nonspecific overreactions that can lead to inflammation. Immunosenescence is the decline in immune defense with aging. Exercise helps to improve efficacy of flu and pneumonia vaccines. Sleep improves the ability to clear viruses more quickly so you're less likely to become symptomatic. Those who eat more fruits and vegetables have a lower risk of getting an upper respiratory tract infection like the common cold. Those eating five or more servings of fruits and vegetables had an 82% greater protective antibody response to a flu vaccine than those who didn't. Disappointing trial results for echinacea. Kiwifruits, broccoli significantly shorten flu duration. Maybe seaweed and soy milk. Mushrooms, particularly shiitake. Nutritional yeast. Green tea. Fibre.

Joints

Athletic injuries to the knees are a well established risk factor for osteoarthritis later in life. At the same time, physical inactivity can put your knees at risk, not only because weakened muscles make for less stable joints but because cartilage also has a "use it or lose it" characteristic. Exercise is consistently effective for pain relief if you have osteoarthritis.

Dietary factors associated with accelerated progression: higher saturated fat intake, dietary cholesterol, pro-inflammatory omega-6 fat, pro-inflammatory diets.

Reduced acceleration: anti-inflammatory diet, fibre, green tea, strawberries. Ginger, turmeric (1/2 tsp daily). No evidence for glucosamine, chondroitin or collagen.

Muscles

Muscle mass starts to decline after 30 and accelerates after 50 at a loss of 1-2% every year. Not just because people become less active with age. Frailty is comprised of weakness, unintentional weight loss, exhaustion, slow walking speed and low physical activity. The heritability of muscle mass and strength is around 50%. Use it or lose it applies to muscle. Eating high on the Dietary Inflammatory Index has been associated with frailty. The recommended dietary allowance of protein is 0.8g of protein per ideal kg of body weight per day. Extra protein or essential amino acid supplementation appears to have little or no effect on muscle mass, strength or performance when taken alone or added to an exercise regimen. Whey protein stimulates the greatest response in terms of short term muscle protein synthesis but there is no correlation between this and long term changes in muscle mass. Higher fruit and vegetable intake linked to lower frailty. Creatine is effective if combined with exercise.

Skin

The three main constituents that make up the bulk of our skin are collagen, hyaluronic acid and elastin. As we age, the synthesis of collagen and elastin decreases by about 1% a year as does skin thickness. Intrinsic aged skin loses elasticity and develops fine wrinkles but is generally otherwise smooth and unblemished but extrinsic skin becomes leathery, bumpy, blotchy and mottled with coarse wrinkles and furrows. Between 80-90% of facial aging of those with light skin is due to sun exposure.

Fibre for varicose veins. Minimise sun exposure and use sunscreen to prevent skin cancer. Sunscreen to prevent skin aging, retinol to reverse aging. Nicotinamide for wrinkles. Vitamin C for oxidative stress but shelf stable versions aren't effective so buy L-ascorbic acid and mix 3g into 30g water and use a few drops on face daily. Collagen supplements haven't been shown to be effective. Stimulate collagen synthesis by ensuring a daily vitamin C intake of at least 95mg (an orange or a cup of broccoli have 70mg). Foods which may be defensive against wrinkles are antioxidant rich foods, anti-inflammatory foods, anti-glycation foods, fibre rich foods and foods shown to block collagen- and elastin- destroying enzymes such as garlic, turmeric and ginger.

Teeth

Green tea as a mouthwash, with or without added amla? Sugar consumption is considered to be the one and only cause of cavities. Superior dental health among vegetarians may be due to eating fewer pro-inflammatory foods or more anti-inflammatory components like high fibre diets. But vegetarians do have an increased risk of dental enamel erosion due to the consumption of more acidic fruits and vegetables such as citrus and tomatoes. The solution is to rinse out your mouth with water after consuming sour foods or beverages and waiting 30-60 minutes after consumption to allow your teeth to first remineralise. Flossing before brushing is much better for plaque removal than after. Nitrates may play an important antimicrobial role in saliva which alleviates gum inflammation.

Vision

Prevent cataracts with spring greens, spinach and goji berries.

[kowshikdm139]

Unlocking the Secrets of Lifespan Extension: Exploring Strategies for Prolonging Vitality

Introduction:

The Quest for Lifespan Extension

In a world where scientific advancements are continually pushing boundaries, the quest for lifespan extension has become a prominent topic of exploration. As humanity strives to unlock the secrets of longevity, researchers are delving into various strategies aimed at prolonging vitality. This article will explore key approaches and breakthroughs in the field of lifespan extension.

Understanding the Biological Basis:

Decoding the Aging Process

Before diving into strategies for lifespan extension, it's crucial to understand the biological basis of aging. Aging is a complex process influenced by genetic, environmental, and lifestyle factors. At the cellular level, the accumulation of damage, deterioration of DNA, and the decline in cellular functions contribute to the aging phenotype.

Strategies for Lifespan Extension:

Nutritional Interventions

One avenue gaining significant attention is nutritional interventions. Caloric restriction, for instance, has shown promise in extending lifespan in various organisms. Studies suggest that reducing calorie intake without malnutrition activates molecular pathways associated with longevity, promoting cellular repair and resilience.

The Role of Dietary Supplements

Certain dietary supplements, such as resveratrol and NAD+ precursors, have demonstrated potential in promoting cellular health and longevity. These compounds activate sirtuins, a class of proteins linked to various cellular processes, including DNA repair and energy metabolism.

Genetic Approaches

Advancements in genetic engineering have opened new possibilities for extending lifespan. Gene editing technologies like CRISPR-Cas9 enable scientists to manipulate specific genes associated with aging. Researchers are exploring the potential of enhancing cellular repair mechanisms and slowing down the aging process through targeted genetic interventions.

Telomere Extension and Cellular Senescence

Telomeres, protective caps at the end of chromosomes, naturally shorten with each cell division, contributing to cellular aging. Scientists are investigating ways to extend telomeres, delaying cellular senescence. Strategies like telomerase activation aim to maintain telomere length and promote cellular longevity.

Senolytics - Clearing Aging Cells

Senescent cells, which have ceased to divide and accumulate with age, contribute to tissue dysfunction. Senolytic drugs target and eliminate these aging cells, promoting tissue rejuvenation. Research in this area explores the potential of senolytics in delaying age-related diseases and extending healthy lifespan.

Hormonal Interventions

Hormones play a crucial role in regulating various physiological processes, and their decline with age is associated with several aging-related issues. Hormonal interventions, such as hormone replacement therapy, are being investigated for their potential in extending healthspan and mitigating age-related conditions.

Growth Hormone and Longevity

Studies on growth hormone suggest a complex relationship with aging. While excessive levels may accelerate aging, optimizing growth hormone levels in a controlled manner may have positive effects on muscle mass, fat distribution, and overall vitality.

Ethical Considerations and Challenges:

Balancing Progress and Ethical Concerns

As scientists make strides in the quest for lifespan extension, ethical considerations become paramount. Questions surrounding access to life-extending technologies, potential socio-economic implications, and the definition of a "good" or "meaningful" life in an extended lifespan scenario must be addressed.

The Challenge of Long-Term Studies

Conducting long-term studies on lifespan extension presents a significant challenge. The human lifespan is inherently lengthy, making it difficult to observe the effects of interventions over several decades. Researchers face the task of designing studies that can provide meaningful insights within a reasonable timeframe.

Conclusion:

Navigating the Future of Lifespan Extension

In the pursuit of unlocking the secrets of lifespan extension, a multidisciplinary approach involving genetics, nutrition, and hormonal interventions is revealing promising avenues. As science continues to advance, ethical considerations will guide the responsible development and implementation of life-extending technologies. The journey towards a longer, healthier life is complex, but the potential benefits for individuals and society as a whole make it a journey worth taking.

[dog-years]

Revitalizing Canine Health: The Role of NAD+ for Dogs

In the realm of pet health and longevity, there's a captivating molecule making waves - Nicotinamide Adenine Dinucleotide, better known as NAD+. It's not just a buzzword in human wellness; it's also a vital component in enhancing the well-being of our canine companions. In this article, we embark on a journey into the world of NAD+ for dogs, exploring its significance, how it contributes to revitalizing canine health, and the potential it holds for our furry friends.

Understanding NAD+

Before we dive into the role of NAD+ in canine health, let's unravel the mystery of this remarkable molecule. NAD+ is a coenzyme found in all living cells, including those of our beloved dogs. Its primary function is to facilitate crucial biochemical reactions, which are the building blocks of life.

The Multifaceted Role of NAD+

NAD+ wears many hats in the world of cellular health, and these roles are just as essential for dogs as they are for humans:

Energy Production: NAD+ plays a pivotal role in cellular respiration, the process by which cells convert nutrients into energy. This energy production is the cornerstone of vitality and function in all living organisms.

DNA Repair: NAD+ is a key player in DNA repair mechanisms. It assists in maintaining the integrity of genetic information, preventing mutations that can lead to diseases and the aging process.

Cellular Defense: NAD+ contributes to the body's defense mechanisms against oxidative stress and damage. It helps regulate the activity of enzymes that neutralize harmful free radicals.

NAD+ and Canine Aging

Aging is an inevitable part of life, and it affects our canine companions just as it does us. As dogs age, their NAD+ levels naturally decline, much like in humans. This decline has profound implications for cellular function, metabolism, and overall health. The consequences of reduced NAD+ levels in aging dogs can manifest in various ways, including:

Decreased energy levels

Impaired DNA repair

Increased susceptibility to diseases

Slower metabolism

Revitalizing Canine Health with NAD+

Recognizing the pivotal role of NAD+ in maintaining optimal health, researchers have embarked on a journey to develop NAD+ boosting treatments for dogs. These treatments aim to elevate NAD+ levels in a dog's body, effectively revitalizing their health and well-being. Key components of these treatments include:

NAD+ Precursors: Precursors like nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN) can be supplemented to increase NAD+ levels in dogs. These precursors are converted into NAD+ within the body, acting as a catalyst for enhanced cellular vitality.

Senolytics: Senolytics are compounds designed to target and remove senescent cells - cells that no longer function optimally due to aging. By eliminating these non-functional cells, NAD+ boosting treatments can rejuvenate tissue and organ function.

The Promise of NAD+ for Canine Health

NAD+ boosting treatments hold tremendous promise for revitalizing canine health. With increased NAD+ levels and the subsequent improvement in cellular function, these treatments offer various potential benefits for dogs, including:

Restored vitality and energy

Enhanced DNA repair mechanisms

Potential extension of the canine lifespan

Increased resistance to age-related diseases

In conclusion, NAD+ isn't just a molecule for human wellness; it's a game-changer in the world of canine health and longevity. As research in this field continues to evolve, NAD+ boosting treatments have the potential to redefine how we approach aging and well-being for our furry companions. So, the next time you look into those soulful canine eyes, know that NAD+ may hold the key to revitalizing their health and ensuring they enjoy a happy, active life by your side.

[manywhelps-handleit]

Rapamycin's Impact on Aging through mTOR Inhibition

Aging is a complex biological process characterized by a progressive decline in the physiological functions of an organism over time, resulting in an increased susceptibility to diseases and death. Researchers have long sought ways to extend human lifespan and improve healthspan, the period of life spent in good health. One intriguing avenue of investigation involves the mammalian target of rapamycin (mTOR) pathway, a key regulator of cellular processes linked to aging. Rapamycin, a drug initially developed as an immunosuppressant, has emerged as a promising intervention to delay aging and age-related diseases by inhibiting mTOR signaling. In this article, we explore the molecular mechanisms behind rapamycin's effects on aging via mTOR inhibition and discuss its potential implications for human longevity and healthspan.

The mTOR Pathway and its Role in Aging:

mTOR is a highly conserved protein kinase that functions as a central regulator of cellular metabolism, growth, and proliferation in response to various environmental cues, such as nutrient availability, growth factors, and energy status. The mTOR pathway forms two distinct protein complexes: mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). Of particular interest in aging is mTORC1, which plays a pivotal role in the regulation of cell growth and protein synthesis.

During aging, mTORC1 activity tends to increase, contributing to the accumulation of cellular damage and impairing cellular autophagy – the process by which cells remove damaged components and maintain cellular homeostasis. Dysregulated mTOR signaling has been associated with a variety of age-related diseases, including cancer, neurodegeneration, and cardiovascular disorders.

Rapamycin and its Mechanism of Action:

Rapamycin, also known as sirolimus, is a natural product originally discovered in the soil of Easter Island (Rapa Nui). It was initially developed as an immunosuppressive agent to prevent organ transplant rejection. Later, scientists discovered its ability to extend the lifespan of yeast, worms, flies, and mice. Rapamycin functions by binding to the protein FKBP12 and forming a complex that inhibits mTORC1 activity, thereby reducing protein synthesis and promoting autophagy.

Rapamycin's Impact on Cellular Senescence:

Cellular senescence is a state of irreversible growth arrest that cells enter into as a response to various stressors, such as DNA damage or telomere shortening. While senescence can serve as a protective mechanism against cancer, the accumulation of senescent cells can contribute to tissue dysfunction and age-related diseases.

Rapamycin has been shown to induce selective elimination of senescent cells, a process known as "senolytic" activity. By clearing senescent cells, rapamycin promotes tissue rejuvenation and improves the overall health of aging organisms.

Enhancing Autophagy and Cellular Maintenance:

Autophagy is a cellular process responsible for recycling damaged organelles and macromolecules, thereby maintaining cellular homeostasis and preventing the accumulation of toxic substances. As mTOR is a negative regulator of autophagy, rapamycin's inhibition of mTORC1 stimulates autophagic activity, leading to improved cellular maintenance and stress resistance.

Impact on Age-Related Diseases:

Studies in animal models have demonstrated that rapamycin treatment can extend lifespan and improve healthspan in various species. Rapamycin has been linked to the prevention and treatment of age-related diseases, including neurodegenerative disorders (e.g., Alzheimer's and Parkinson's), cardiovascular diseases, and certain types of cancer.

Caloric Restriction Mimetic:

Caloric restriction (CR) has long been considered one of the most robust interventions to extend lifespan and improve healthspan across species. Interestingly, rapamycin mimics some of the effects of CR by reducing nutrient signaling and promoting autophagy. This has led researchers to label rapamycin as a "caloric restriction mimetic" and explore its potential as an anti-aging intervention.

Potential Side Effects and Challenges:

While rapamycin shows great promise in delaying aging and extending lifespan, there are potential side effects and challenges associated with its use. Long-term rapamycin treatment may weaken the immune system and increase the risk of infections. Furthermore, the precise dosing and timing of rapamycin administration need to be carefully optimized to achieve the desired anti-aging effects without undue side effects.

Translating Rapamycin Research to Human Longevity:

Despite its potential benefits, there are still several hurdles to overcome before rapamycin can be widely used as an anti-aging intervention in humans. Clinical trials are ongoing to determine the optimal dose and treatment regimen for different age groups and to assess potential side effects and drug interactions.

Conclusion:

Rapamycin's effects on aging via mTOR inhibition offer a compelling avenue for research into anti-aging interventions. By modulating the mTOR pathway, rapamycin enhances cellular maintenance, reduces cellular senescence, and improves autophagy, thereby potentially extending lifespan and improving healthspan. 

However, further research is necessary to fully understand the complexities of rapamycin's impact on aging and to develop safe and effective interventions for humans. As the field of geroscience continues to advance, ageing people can now buy rapamycin and related compounds may play a vital role in our quest to unlock the secrets of aging and promote healthy aging in human populations.

Rapamycin has been used by many to improve their quality of life as they get into their golden years. 

[longevityboxcuk]

Stay Safe When Buying Supplement Online

A person's safety must always be their top concern while shopping online for dietary supplements of any kind, including those intended for weight loss, muscle growth, protein, and so on. Here are some guidelines to keep in mind while shopping for supplements online:

  While making a purchase, be wary of the firm.

Several retailers offer Senolytics Supplement of varying types. No two businesses are identical in the same way that no two individuals are same. You should exercise caution while dealing with the company. Rule of thumb: Stick with retailers who have earned the FDA's seal of approval. You can also read the reviews of previous customers to spot any warning signs that the firm might not be trustworthy. You should verify the company is on any necessary government lists.

    The BBB is an excellent resource for this type of research. You should not do business with this company if you have any reason to suspect it is not legitimate. It also matters greatly where the company was founded. You should only do business with an organization that is based in a country that has rigorous safety regulations for nutritional Senolytic Activator. Experts advise only purchasing from well-known, established brands when shopping for supplements. Although charging premium costs, most companies adhere to good production methods, thus their products are of a high standard.

  Pay attention to the constituents

When creating their supplements, many companies use a wide variety of resources. To avoid any potential dangers, it is important to check each ingredient used in the production process to make sure they are appropriate for human consumption. It's best to get your doctor's opinion if you have any doubts about whether or not the components are safe. Supplements with potentially dangerous components should be avoided as a general rule.

    Verification agencies' stamps of approval are just as important as the supplement's components, so make sure you look for them. Many groups fall into this category, among of which include NSF International and the United States Pharmacopeia. The supplement's medical claim should alert you to the need to keep an eye out for any clinical studies involving the Nmn Supplements.

  Go over the return policy thoroughly

It's possible that you'd like to send an item back for a variety of reasons, including receiving the wrong item, changing your mind, or experiencing negative side effects. Make sure you get the product from a reputable retailer that offers a return policy before making your purchase. Knowing you could always make a return and receive your money back is reassuring, and the policy serves as further evidence that the company is trustworthy.

  Read the company's policy paper thoroughly and ask questions if necessary, before placing an order. There are certain businesses that will accept returned supplements for a 10% restocking fee. While some businesses will accept returns, those that have been opened are typically rejected.

  Advice for making Nmn Supplement purchases from reputable online vendors. In order to avoid such problems, you should only purchase from a reliable vendor.

[biotech-news-feed]

The authors of a paper published in Pharmaceuticals tested multiple drug-polyphenol combinations to find the ones with the best senolytic properties {1}. Targeting senescent cells in lung diseases ... #BioTech #science

[jhavelikes]

The healthspan of mice is enhanced by killing senescent cells using a transgenic suicide gene. Achieving the same using small molecules would have a tremendous impact on quality of life and the burden of age-related chronic diseases. Here, we describe the rationale for identification and validation of a new class of drugs termed senolytics, which selectively kill senescent cells. By transcript analysis, we discovered increased expression of pro-survival networks in senescent cells, consistent with their established resistance to apoptosis. Using siRNA to silence expression of key nodes of this network, including ephrins (EFNB1 or 3), PI3Kδ, p21, BCL-xL, or plasminogen-activated inhibitor-2, killed senescent cells, but not proliferating or quiescent, differentiated cells.

The Achilles’ heel of senescent cells: from transcriptome to senolytic drugs - Zhu - 2015 - Aging Cell - Wiley Online Library

[transhumanitynet]

Trends in Regenerative Medicine

This is just a taste of what is out there.  A quick snippet of a few of the myriad trends that are coalescing toward a regenerative medicine movement that will transform the world.  Hopefully they provide some useful insight into where we are headed and how we are getting there.  I concocted this article recently as a writing sample for a job.  We will see whether or not I get it but no reason not to let everyone else enjoy the efforts as well.

BioMedical Advances – The future of keeping you healthy.

  By – Matthew Lehmitz

  Imagine regrowing an entire limb or organ using nothing but a handful of your own existing cells.  Consider the possibility of increasing muscle mass simply by injection.  

  We live in an age where machines and living things are ever more closely connected and where bioengineering is looking ever more like classical engineering.

  In march 2020 more than 112,000 people in the United States alone were waiting for organ transplants.  This far outstrips organ donations rates which for all of 2019 stood at 19,267. 

  What if we could cut that number to zero and reduce the wait time from years to months to days or even hours?

  This and more is the promise of modern and future bioengineering technology.  Let us explore more of what life might bring us and what we can bring to life.

  The famous quote from the six million dollar man goes “We can rebuild him, we have the technology”.  This has never been more true.  

  Not only can we now treat damage and slow the progression of time and illness but we have the capability to begin to rebuild and even improve the human body.  

  We can produce new organs or regrow limbs.  We can make new skin or alter the very DNA sequences that underlie our basic functioning.  

  In the future we can end aging and make our bodies even more resistant to disease and damage preventing harm in the first place.    

  3d bioprinting:

  One of the most interesting areas at this time is a field called 3D bioprinting.  

  We’ve all heard or read about 3d printing and the amazing things it can produce in plastic.  Whether you want a model of the USS Enterprise or a new steering wheel for your car it can save a lot of potential time and money.

  This technology is expanding rapidly.  By the year 2022 spending on 3D printers alone is expected to exceed 5.3 Billion USD annually.  

  So what if we converted the printer to create things not of plastic but of cells?  

  This is the question that is answered by the 3D bioprinting revolution.  

  Rather than rely on human donors for transplant organs we can simply turn on a machine primed with cells from the person needing a transplant and let it print away.  

  Of course not all organs are flat and so a biocompatible gel or soft material is used to form a scaffold on which to print.  

These prints can be finished in a matter of days or even less with some bone printing systems able to complete their tasks in as little as two hours.  

  Rejection has long been a problem with transplants.  Acute rejection, where the body rejects the new organ and attacks it as a foreign body,  occurs in up to 30 percent of cases for liver and as high as 20 percent for kidney transplants.

  A huge advantage of using organ printing is that it avoids the issue of rejection by using the patients’ own cells.  Since they are already accepted by the patients’ immune system they are very unlikely to be targeted as foreign bodies.

  Costs for medical procedures are quite high in the United States with Medicare spending exceeding 3.4 Billion annually for Kidney Transplants.  

  3D printing will bring these costs down significantly.  Not only will it be cheaper and easier to find and safely transplant the organs but the technology is cheap as well.

  Many quality 3D printers have fallen below the 300 USD price point.  We can expect to see the 170,000 USD price point of professional bioprinters fall quickly as we have seen with its sibling technology.

  Limb Regeneration:

  How hard would it be to live without a finger?  Without a hand?  Even an entire arm?  Life would certainly be very difficult.  Living with the loss of a limb is a challenge that faces more than 2.1 million Americans every day.  Approximately 400 amputations are performed nationwide on an average day.

  Most people currently use some kind of mechanical prosthesis to provide a limited replacement for the lost limb.  This may soon be changing however, as regrowing limbs becomes more feasible.  

  There are many creatures in nature that regrow lost parts.  It turns out that humans are not substantially different.  

  Studies have shown that babies can regenerate fingertips when they are young and prenatal humans can regrow entire limbs in the womb.  

  This fits with our own evolutionary history as we see a variety of reptiles do this as do some amphibians.  

  We can trace specific gene activation in mammals called lin28a.  This specific gene was found by George Daley and his team at Harvard Medical to result in significant tissue regeneration in mice.

  This only occurred however, when the gene was prevented from switching off and only when the mice were young.   

  So humans have this ability to regenerate and it can be found in other mammals.  It is simply one of the many genes that gets switched off as we age.  What can we do to reactivate it or keep it from leaving us in the first place?

  One amazing solution may be the development of a powder which can be rubbed onto damaged limbs which help them regrow.  While the studies on this have been limited some individuals have seen healthier and more complete limb repair than was otherwise expected.

  Stem cells seem like a strong approach.  They already are pluripotent which means that these cells can be induced to grow into any cell that is needed.  With the right inducement stem cells could be made to grow any organ or tissue in the body.  

  Future stem cell applications could enable the regrowth of an entire arm or leg.  

  Ending Aging:

  No health concern is as serious or omnipresent as aging.  It is the disease that every human is born with and which will eventually claim every person if nothing else does first.

  The plague of aging has followed humanity throughout its entire existence but that is beginning to change.  

Imagine a situation where humans live not just much longer lives but also much healthier ones where aging is a controlled condition or overcome entirely.  

  Many current research trends in gerontology, the study of aging, present a tantalizing perspective suggesting this isn’t just possible but that we can make great strides in this area.

  Genetic modification has become ever more achievable.  Since the development of CRISPr Cas-9 protocols the ability to accurately and reliably alter genomes has been greatly improved.  

  Genes are spectacular things that play such a key role in every facet of our lives that any ability to alter them can have amazing effects.

  A few genes have already been identified as having strong connections with aging.  

  One of them called AAV9-TERT is a gene tied to the production of telomerase.  

  Telomerase is a chemical that is responsible for managing the length of telomeres.  ‘Caps’ of a sort on the end of genetic sequences.  

  As we age our telomeres become shorter and can lead to genetic damage and decrease cellular functioning.  Increasing the length and safety of these coverings can help reduce effective biological age.

  This approach has been reported to improve the lifespan of mice by as much as thirty percent.  

  Work in the area of senolytics is very exciting.  Senolytics is the study of ways we can remove senescent cells from the body.  

  Senescent cells are those that are working poorly or not at all.  They take up resources and can be detrimental to nearby cells. 

  Senolytic therapies endeavor to remove these cells while leaving healthy tissue undamaged and free of their negative influence.

  Adding senolytic approaches to gene therapies has seen lifespans of mice increased by as much as 36 percent.

  Conclusion:

  Through constant effort and improvement of our experience and techniques we will continue to live longer and healthier lives.

  We will grow and harvest new and healthy organs, edit our genes to better serve our needs, live long and healthy lives.

  These will all have powerful impacts on society and one of the most significant adjustments that will have to be made across all sectors is how to deal with this.

  The health care sector will see huge shifts and disruption in its market as demand for these new treatments increases while more traditional treatments and approaches fall away.

  There is a massive business opportunity in this as a result and many new startups are moving into the anti-aging sector.  They will be at the forefront of next phase developments and will not only profit from the changing markets but provide great profit to humankind.

  Society will also see innovation racing ahead as we learn to deal with a whole new paradigm for generations, education, experience, sports and all other facets of human striving.  It may be difficult but will ultimately see us become stronger as friends and as people for the effort.  

Trends in Regenerative Medicine was originally published on transhumanity.net

[senolyfe-blog]

SenoStack is a Senolytic Cocktail of the most important flavonoid compounds (Fisetin, Curcumin, Quercetin and Luteolin) that have been shown to support Senolytic activity in dysfunctional senescent cells that accumulate with age.

http://bit.ly/SenoStack

[wisepowder]

Pure Fisetin Powder: A Dietary Antioxidant In Aging and Longevity

Fisetin is a common botanical polyphenol and flavonoid found in a wide variety of plants, fruits and vegetables including strawberries, apples, persimmons, onions and cucumbers. Fisetin is considered a plant pigment imparting many fruits and vegetables, such as strawberries, with their characteristic color and appearance. Fisetin has a very similar molecular structure as the more popular plant flavonoid and dietary supplement Quercetin. Unlike Quercetin, however, Fisetin may be a senolytic and perhaps one of the most powerful senolytics known.

Manufacture: Batch Production

Package: 1KG/bag, 25KG/drum

1.What is Fisetin?

2.The Mechanism Of Action of Fisetin: How does Fisetin work?

3.What food contains Fisetin?

4.What are the benefits of Fisetin?

5.Fisetin Vs Quercetin: is fisetin the same as quercetin?

6.Fisetin Vs Resveratrol: is fisetin better than resveratrol?

7.Fisetin and weight loss

8.How much fisetin should I take: The fisetin dosage?

9.What are the side effects of fisetin?

10.Fisetin powder and fisetin supplements online

Fisetin Chemical Base Information Base Information

Name: Fisetin Powder

CAS: 528-48-3

Purity: 65%，98%

Chemical name: 2-(3,4-Dihydroxyphenyl)-3,7-dihydroxy-4H-1-benzopyran-4-one

Synonyms: 2-(3,4-dihydroxyphenyl)-3,7-dihydroxychromen-4-one , 3,3′,4′,7-Tetrahydroxyflavone ,5-Deoxyquercetin , Natural Brown 1 , CI-75620 , NSC 407010 , NSC 656275 , BRN 0292829 ,

Cotinin , 528-48-3 (anhydrous)

Molecular Formula: C15H10O6

Molecular Weight: 286.24

Melting Point: 330° C (dec.)

InChI Key: GYHFUROKCOMWNQ-UHFFFAOYSA-N

Form: Solid

Appearance: Yellow Powder

Half Life: /

Solubility: Soluble to 100 mM in DMSO and to 10 mM in ethanol

Storage Condition: −20°C for long time

Application: Fisetin is a potent sirtuin activating compound (STAC), antiinflammatory and anticancer agent

Testing Document: Available

Flavonoid polyphenols are commonly used for their antioxidant properties. Their main source is fruits and vegetables that are consumed regularly, by

millions worldwide. Due to their health benefits, flavonoids have also become key ingredients in different dietary supplements, especially resveratrol.

Recent studies have found a new flavonoid namely fisetin, which is believed to be the most potent among the other flavonoids used as a dietary

supplement. Fisetin powder or Fisetin supplements have since increased in demand due to their health benefits.

What is Fisetin?

Fisetin is a flavonoid polyphenol that acts as a yellow pigment in plants. Originally discovered in 1891, fisetin is found in many fruits and vegetables such

as persimmon and strawberries. Although it has been around for a long time, it was only recently that the fisetin benefits were discovered and made it

stand out compared to other supplements. Moreover, it was the potential medicinal benefits of fisetin powder that encouraged research into the topic.

Although it has been studied and the fisetic benefits and fisetin side effects have been realized, there is still a lot that scientists haven’t been able to

understand about the flavonoid.

The Mechanism Of Action of Fisetin: How does Fisetin work?

Fisetin powder works through multiple pathways in the human body. Fisetin especially works on the antioxidants levels in the body and this is one of its

major benefits. It fights against free radicals, which are unstable ions that will participate in harmful chemical reactions to harm the body. Fisetin’s

antioxidant properties allow it to neutralize these free radicals and hence, reduce the oxidative stress that the body is under.

Another mechanism of action of fisetin is that it blocks the NF-KB pathway. This pathway is important for the production and release of

pro-inflammatory cytokines and eventually, inflammation. NF-KB is a pro-inflammatory pathway that induces gene transcription to synthesize the

inflammatory proteins. When overtly activated, the NF-KB pathway plays an important role in cancer development, allergies, and autoimmune diseases.

Fisetin powder blocks this pathway, hence, acting as an anti-inflammatory supplement.

Fisetin powder also blocks the action of the mTOR pathway. This pathway, much like the NF-KB pathway, is involved in the development of cancer,

diabetes mellitus, obesity, and neurodegenerative diseases. mTOR pathway causes the cells to panic as they struggle to meet the energy demands of

the pathway, resulting in an excessive workload on the cells. What this means is that cells are overworking and producing metabolic waste but there

isn’t sufficient time to clean up the waste resulting in the accumulation of waste. This can be detrimental to cellular health and the blockage of this

pathway by fisetin supplement is how fisetin helps manage obesity, diabetes, and cancer.

Apart from these major mechanisms of action, fisetin is also able to inhibit the activity of lipid-degrading enzymes, lipoxygenases. It also inhibits matrix

metalloproteinases or the MMP family of enzymes. These enzymes are crucial for cancerous cells to be able to invade other tissues, however, with the

use of fisetin powder, that is no longer possible.

What food contains Fisetin?

Fisetin is a plant-based flavone that is primarily extracted from apples and strawberries. It is a pigment of yellow and ochre color in plants, meaning that

most fruits and vegetables of that color are rich in fisetin. Fisetin, in plants, is synthesized from the amino acid phenylalanine, and the accumulation of

this flavone in plants is highly dependent on the environment of the plant. If the plant is exposed to shorter wavelengths of UV rays, then there is an

increase in the production of fisetin. Fisetin powder is made from the isolation of fisetin from the following plant sources.

Plant sources Amount of Fisetin (μg /g)

Toxicodendron vernicifluum: 15000

Strawberry: 160

Apple: 26

Persimmon: 10.6

Onion: 4.8

Lotus root: 5.8

Grapes: 3.9

Kiwifruit: 2.0

Peach: 0.6

Cucumber: 0.1

Tomato: 0.1

What are the benefits of Fisetin?

Fisetin benefits are quite a few, and they have all been seen on animal models. No research has been able to conclusively determine these benefits in

humans as most studies are still in the clinical phase. The different benefits of fisetin include:

Anti-Aging

Aging of the body is marked by a net increase in senescent cells, that are no longer able to divide. These cells release inflammatory signals, which

results in the complications of aging most commonly seen. Most age-related disorders are due to abhorrent inflammation in the body promoted by the

senescent cells. Fisetin powder consumption targets these cells and removes them from the body, hence, reducing inflammation and slowing down the

aging process.

Diabetes management

In animal models, fisetin supplement has been shown to reduce blood sugar levels significantly. This effect of fisetin comes from the flavonoid’s ability

to increase insulin levels, increase glycogen synthesis, and decrease the liver’s ability to initiate gluconeogenesis. Basically, fisetin acts on every pathway

in the body that results in glucose production and stops those while activating the pathways that either store or use up the glucose in the bloodstream.

Anti-Cancer

The anti-cancer effects of fisetin powder differ based on the type of cancer. In a study performed on prostate cancer, fisetin was able to reduce the

growth of cancer by blocking testosterone and DHT receptors, which are important for the growth of prostate cancer. In another study where the

cancer being studied was lung cancer, fisetin supplements were able to increase antioxidants in the blood that had been reduced by tobacco use.

Fisetin was also able to reduce the growth of lung cancer by 67 percent on its own, and 92 percent when combined with a chemotherapy drug. When

used in colon cancer, fisetin significantly reduced the inflammation associated with colon cancer. The study, however, did not mention any effect of

fisetin on cancer growth.

Neuroprotective

When older rats with age-related decline in cognition were given fisetin supplement, there was a significant improvement in their cognitive skills and

memory. In another study, animal models were exposed to neurotoxic substances and then given a fisetin supplement. The test subjects were found to

have not experienced any memory loss due to the supplement. However, it is not known if fisetin can cross the human blood-brain barrier with the

same efficiency as the mice blood-brain barrier.

Fisetin is also neuroprotective in the sense that it prevents the development of neurodegenerative disorders such as Alzheimer’s by reducing the

accumulation of harmful proteins in the brain. Similarly, mice with ALS showed an improvement in their balance and muscle coordination after being

given fisetin powder. They also experienced a prolonged life span than what was expected.

Cardioprotective

Researchers studied the effect of fisetin powder on the cholesterol levels of rats who were fed a high-fat diet. Total cholesterol and LDL levels were

found to have decreased significantly whereas HDL levels almost doubled. The hypothesized mechanism through which fisetin rids the body of

cholesterol is believed to be increased release of it into bile. The reduced cholesterol, overall, has a cardioprotective effect.

All these fisetin benefits point toward anti-aging and longevity of life which should be enough to promote more clinical studies so that the compound

can be approved for medicinal use.

Fisetin Vs Quercetin: is fisetin the same as quercetin?

Quercetin and Fisetin are both plant flavonoids or pigments that are well known for their anti-inflammatory and anti-oxidant properties. They both also

have significant anti-aging properties, which they perform by clearing senescent cells from the body. Fisetin powder, however, has been shown to clear

out the cells with increased efficacy and potency than quercetin.

Fisetin Vs Resveratrol: is fisetin better than resveratrol?

Resveratrol is a polyphenol that is also quite popular for its anti-oxidant properties. Taking quercetin and resveratrol results in a synergistic effect on the

body, although quercetin is more potent at mediating inflammation and managing insulin resistance. Since Fisetin is better at performing these

functions than quercetin, it can be concluded that fisetin supplement is better than resveratrol supplements.

Fisetin and weight loss

Researchers studied the effect of fisetin powder on fat accumulation in the body and it was found that it blocks certain pathways to reduce diet-related

obesity. It targets the mTORC1 signaling pathway. This pathway is important for cell growth and lipid synthesis, hence, inducing fat accumulation in the

body.

How much fisetin should I take: The fisetin dosage?

Fisetin dosage ranges between 2 mg to 5 mg, per kilogram of weight, however, this is not the recommended guideline for the dosage. There is no

specific dosage recommendation for fisetin usage, and speaking to a medical professional would help in determining a fisetin dosage range, specific to

one’s own conditions. In one of the studies conducted with the purpose of assessing the effect of fisetin powder on inflammation caused by colon

cancer, 100 mg per day was required to notice a significant reduction in inflammation.

What are the side effects of fisetin?

Fisetin only recently became the subject of multiple studies and different pieces of research. This late interest in the flavonoid means that most studies

performed have been on animal models or in a lab setting. Not many human studies have been performed to conclusively determine the potential side

effects and toxicities of the supplement. Animal models on exposure to high doses of fisetin supplement did not show any adverse effects, pointing

towards the safety of the supplement.

However, it is important to remember that lack of side effects in animal models does not mean that the risk of side effects in humans does not exist. To

reach that conclusion, more clinical studies need to be performed. In one study that was performed on cancer patients to assess the efficacy of fisetin

powder in managing the symptoms of cancer, both placebo and control groups reported gastric discomfort. Since the side effect was present in both

groups, and both groups were undergoing chemotherapy at the same time, it is hard to conclude that fisetin powder consumption can cause gastric

discomfort.

Fisetin powder may not have any reported side effects but it does interact with certain drugs, resulting in altered metabolism of those drugs. Fisetin

was found to reduce the blood sugar levels in animal models, which is quite a benefit on its own. But when taken in conjunction with anti-diabetic

medications, the glucose-reducing effect of both, the supplement and the drug could be exaggerated. This could result in several health complications.

Fisetin powder is metabolized by the liver, in the same way, that blood thinners are metabolized. Due to this, it is hypothesized that these two would

interact with each other and Fisetin powder would increase the effects of blood-thinning agents.

Fisetin powder and fisetin supplements online

Fisetin powder can be bought online from different fisetin powder manufacturers, in quantities based on the specific need. Buying fisetin bulk amounts

can help with the pricing as well. Fisetin’s price is not that out of range, and it is in the same range as other flavonoid supplements.

When looking to buy a fisetin supplement, it is important to thoroughly look through fisetin powder manufacturers and their manufacturing process.

This is to ensure that proper safety guidelines and manufacturing protocols are followed during the production of the fisetin supplement. It is crucial to

buy pure fisetin powder as it makes for the best fisetin supplement. If the supplier does not follow safety protocols in the extractions and synthesis of

fisetin, the end-product can be contaminated or tainted with ingredients that are either harmful to human health or have no effect on human health,

whatsoever. Either way, the fisetin benefits would not be experienced despite taking the supplement for a long time.

It is always important to look into the ingredients of the fisetin powder being purchased and the concentration ratio of these ingredients to ensure that

pure fisetin powder is being purchased. If this distinction is not made, then there is a huge likelihood of increased fisetin side effects and/or reduced

fisetin benefits, overall.

References

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5527824/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261287/

https://pubmed.ncbi.nlm.nih.gov/29275961/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780350/

https://link.springer.com/article/10.1007/s10792-014-0029-3

https://pubmed.ncbi.nlm.nih.gov/29541713/

https://pubmed.ncbi.nlm.nih.gov/18922931/

https://pubmed.ncbi.nlm.nih.gov/17050681/

https://pubmed.ncbi.nlm.nih.gov/29559385/