#nippon no DNA

[yume-kara-samete]

Nippon no D・N・A! - BEYOOOOONDS

BEYOOOOONDS CONCERT TOUR 2024 AUTUMN "DISCOOOOOTHEQUE"

[haropro-confessions]

The very first time that I heard Nippon no DNA I was eating cheesecake and I heard the part when they say "MONGOLOID DNA" and I almost choked on it. I was like: "TF did they just say?!" as I understand that it is not a nice word to use.

[the-northcap-university]

Leveraging the Power of Sports Sponsorship: Impact on Brand Equity in the IPL Era

In recent years, sports sponsorship has emerged as a potent marketing strategy for brands worldwide. The Indian Premier League (IPL), one of the most-watched cricket tournaments globally, has become a prime arena for brands to enhance their visibility and build brand equity. This blog explores the role of sponsoring sports events like the IPL in bolstering brand equity, supported by data and sources.

Increased Brand Exposure: Sponsoring the IPL offers brands unparalleled visibility to a vast and diverse audience. According to a report by Nielsen, the IPL 2020 garnered a cumulative reach of 405 million viewers, making it a lucrative platform for brand exposure (Source: Nielsen Sports). With matches broadcasted globally and extensive media coverage, sponsors gain widespread visibility, thereby increasing brand awareness among a broad demographic.

Enhanced Brand Recall and Recognition: Associating with a high-profile sporting event like the IPL helps brands to establish strong associations in consumers' minds. A study conducted by Kantar revealed that IPL sponsors experienced a 17% increase in brand recall compared to non-sponsors (Source: Kantar). Consistent brand presence throughout the tournament, including on player jerseys, stadiums, and advertisements, reinforces brand recognition and ensures that the brand stays top-of-mind among consumers.

Positive Brand Image and Perception: Sponsoring sports events like the IPL allows brands to align themselves with the passion and excitement of sports, fostering positive brand associations. Research indicates that consumers perceive brands sponsoring sports events as dynamic, youthful, and energetic (Source: Marketwatch). This positive brand image translates into increased consumer trust, loyalty, and preference, ultimately contributing to enhanced brand equity.

Opportunities for Consumer Engagement: Sports sponsorships offer brands unique opportunities for direct consumer engagement and interaction. Through various activation activities such as fan zones, contests, and social media campaigns, sponsors can create immersive brand experiences for fans. According to a study by Ipsos, 75% of fans feel more positively about sponsors who engage them beyond traditional advertising (Source: Ipsos). Such interactive initiatives not only strengthen brand-consumer relationships but also generate valuable user-generated content, amplifying brand reach and engagement.

Financial Performance and Market Share: Research suggests a positive correlation between sports sponsorship and financial performance. A study published in the Journal of Advertising Research found that companies investing in sports sponsorship experienced an increase in market share and stock prices (Source: Journal of Advertising Research). The heightened visibility and positive brand associations derived from sponsoring sports events like the IPL contribute to improved brand performance and competitiveness in the market.

Long-Term Brand Building and Loyalty: While the immediate impact of sports sponsorship is evident, its long-term benefits in brand building and loyalty are equally significant. According to a report by Repucom, 65% of sports fans are more likely to purchase products from brands actively involved in sponsoring sports events (Source: Repucom). By nurturing emotional connections with consumers through shared experiences and values, brands can cultivate enduring brand loyalty, driving sustained growth and profitability.

Here are some of the prominent team-brand associations in the IPL:

Mumbai Indians:

Principal Sponsor: Samsung

Official Partners: Marriott Bonvoy, Colors, Usha, Jio, Kingfisher, Dream11, Burger King, Kotak, William Lawson's, Boat, DNA Networks, Radio City, Performax, Astral Pipes

Chennai Super Kings:

Principal Sponsor: Muthoot Group

Official Partners: Equitas Small Finance Bank, Gulf Oil, Nippon Paint, EUME, FanCode, Dream11, Boat, SEVEN, Astral Pipes, Usha, Nippon India Mutual Fund

Kolkata Knight Riders:

Principal Sponsor: BYJU'S

Official Partners: Lux Cozi, Astral Pipes, Dream11, Jio, Kingfisher, Greenply, Royal Stag, Kotak, Nokia, Medimix, Colgate, Asian Paints, Royal Stag, TV9 Bangla, Kotak, and Fancode

Royal Challengers Bangalore:

Principal Sponsor: Muthoot Fincorp

Official Partners: Duroflex, Astral Pipes, Kotak, Jio, Max Life Insurance, Exide, Singha, Mai Dubai, Kingfisher, Dream11, and Nissin.

Delhi Capitals:

Principal Sponsor: JSW Group

Official Partners: APL Apollo, Dream11, Jio, FanCode, Boat, Kotak, Colgate, BKT Tires, Livinguard, Fever FM, Nerolac, and EbixCash.

Rajasthan Royals:

Principal Sponsor: TV9 Bharatvarsh

Official Partners: Kei Wires & Cables, Astral Pipes, Kingfisher, Jio, Red Bull, Dream11, DafaNews, Dream11, FanCode, Acko General Insurance, and Jai Raj Steel.

Sunrisers Hyderabad:

Principal Sponsor: JK Lakshmi Cement

Official Partners: Ralco Tyres, Astral Pipes, Dream11, Jio, TCL, Colgate, Kia Motors, Nerolac, BKT Tires, and Jai Raj Steel.

Punjab Kings:

Principal Sponsor: EbixCash

Official Partners: BKT Tires, Colgate, Astral Pipes, Dream11, Jio, Fancode, Tata Motors, and Jai Raj Steel.

These associations not only provide financial support to the teams but also help brands in gaining exposure and reaching out to a wide audience during the IPL season.

Sponsoring sports events like the IPL offers brands a powerful platform to enhance brand equity and achieve strategic marketing objectives. The extensive reach, heightened visibility, and positive brand associations derived from sports sponsorships contribute to increased brand awareness, recall, and preference among consumers. Moreover, the opportunities for consumer engagement and long-term brand building further solidify the value proposition of sports sponsorship for brands. As the global sports sponsorship landscape continues to evolve, brands leveraging the inherent synergy between sports and marketing stand to reap substantial rewards in terms of brand equity and financial performance.

Authored by Dr. Kanchan Yadav Assistant Professor, Department of Management & Commerce Studies, The NorthCap University

[bhushans]

Unveiling the Power of Data: Global Loop-Mediated Isothermal Amplification Market

The global loop-mediated isothermal amplification market is projected to be worth $106.6 million in US dollars worldwide. Between 2024 and 2034, the market is anticipated to expand at a typical rate, with a CAGR of 4.9%. The industrial value of loop-mediated isothermal amplification is expected to reach US$ 178.4 million by the end of the

Loop-mediated isothermal amplification is a nucleic acid-based point-of-care diagnostic method that requires fingerpick quantities of cell lysis, nucleic acid extraction, and purification chemicals before amplification can begin. Loop-mediated isothermal amplification is acknowledged as a dependable and potent isothermal amplification technique with the aim of identifying pathogens in patient samples. A developing global market is being seen for loop-mediated isothermal amplification as a viable substitute for the conventional PCR approach, thanks to breakthroughs and improvements in amplification technology. Moreover, there is a growing need for loop-mediated isothermal amplification methods due to the early detection of chronic disorders.

Loop-mediated isothermal amplification has been used in more research publications, and its applications in a wider range of sectors have grown as well. Significant advancements in Amplicon detection support the market's expansion even more. Monitoring loop-mediated isothermal amplification in real time Amplicon now makes use of pH-sensing, optical, electrical, and electrochemical methods. The market for loop-mediated isothermal amplification has been steadily expanding as a result of these developments in amplification technology.

“Global sales of loop-mediated isothermal amplification will be stimulated for the forecast period due to the growing application of loop-mediated isothermal amplification in multiple fields such as environmental science, bio-medicine, food and beverage, and others,” according to an FMI analyst.

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Key Takeaways:

Rapid diagnosis of chronic diseases and improvements in Amplicon detection is expected to drive the market.

Demand of loop-mediated isothermal amplification in China to witness a surge over the forecast period.

The U.S. loop-mediated isothermal amplification market will experience significant growth propelled by adoption of microfluidic technology.

Based on technology, microfluidic segment will be popular for the forecast period.

 Lack of versatility and absence of an internal PCR inhibition control limits market growth.

Competitive Landscape 

Jena Bioscience GmbH, HiberGene Diagnostics, Meridian Bioscience, Inc., Mast Group Ltd., Excellgen, Inc., Merck KGaA, QIAGEN N.V, NIPPON GENE CO., LTD, New England Biolabs, Bio-Rad Laboratories, Inc., Thermo Fisher Scientific, and others are some of the major players in the loop-mediated isothermal amplification market that are profiled in the full version of the report.

Major organizations in the market are employing various tactics to expand their product portfolio as well as to increase their regional dominance. These businesses are focused on using aggressive pricing strategies to gain a competitive advantage in the market. Some global market players are investing in research and development agreements to increase product development, adopting distribution & licensing agreements with regional players, and establishment of network of distribution and marketing agreements to ensure availability of products to different end-users globally.

Key Segments:

Loop-mediated Isothermal Amplification Market by Product:

Instruments

Incubation Systems

Turbidimeters

Agarose Gel Electrophoresis

Fluorescence Measuring Systems

Kits & Reagents

DNA Polymerase

Primer Mix

DNA Polymerase & Primer Mix

Dyes

Other Reagents

Loop-mediated Isothermal Amplification Market by Technology:

Microfluidic Technology

Advanced Infrared Optical Technology

Modern Microsystem Technology

Loop-mediated Isothermal Amplification Market by Application:

Diagnostic Purposes

Research Purposes

Loop-mediated Isothermal Amplification Market by End User:

Hospital Laboratories

Research and Academic Institutes

Diagnostic Centers

Loop-mediated Isothermal Amplification Market by Region:

North America Loop-mediated Isothermal Amplification Market

Latin America Loop-mediated Isothermal Amplification Market

Europe Loop-mediated Isothermal Amplification Market

East Asia Loop-mediated Isothermal Amplification Market

South Asia & Pacific Loop-mediated Isothermal Amplification Market

Middle East & Africa (MEA) Loop-mediated Isothermal Amplification Market

[helthcareheven]

Code Masters: DNA Synthesizer Innovations Driving Discovery

The DNA synthesize market has experienced significant growth in the recent years, owing to increased adoption of DNA synthesizers in gene synthesis applications. DNA synthesizers are used to produce both short and long strands of customized DNA through solid-phase phosphoramidite chemical synthesis techniques. Ability of DNA synthesizers to synthesize DNA sequences from scratch has enabled advancements in research and production of therapeutic genes and oligonucleotides. DNA synthesizers offer high specificity and reliability compared to traditional gene synthesis methods. Rising demand for DNA synthesis technologies in molecular diagnostics, genomics, and development of new therapies is a major factor fueling investments in DNA synthesizers globally. The Global DNA Synthesizer Market is estimated to be valued at US$ 4.3 Bn in 2024 and is expected to exhibit a 20 % CAGR over the forecast period 2023 to 2030.

Key Takeaways

Key players operating in the DNA Synthesizer market are Thermo Fisher Scientific, Merck KGaA, Agilent Technologies, Danaher Corporation (Integrated DNA Technologies), GenScript Biotech Corporation, Eurofins Genomics, LGC Limited, BioAutomation Corporation, Bio-Rad Laboratories, Twist Bioscience Corporation, Hamilton Company, New England Biolabs, Nippon Genetics, LGC Biosearch Technologies, DNA Script, Ribbon Biolabs GmbH, and Camena Bioscience. Thermo Fisher Scientific dominates the global DNA synthesizer market with a market share of over 30% and has a strong geographical presence through its Oligo and Gene synthesis business.

The global demand for DNA synthesizers is growing rapidly due to increasing adoption of DNA synthesis techniques in molecular diagnostics, discovery research, and advancement of genome editing technologies. DNA synthesizers are increasingly being used to produce synthetic genes, gene fragments, siRNAs, shRNAs, DNA primers, oligonucleotides and more. Rising research in areas of synthetic biology, cancer genomics and agriculture biotechnology is propelling market growth.

Geographical expansion of key DNA synthesizer manufacturers across regions such as North America, Europe, Asia Pacific and South America is another factor contributing to growth of the global market. Manufacturers are enhancing their global distribution networks and establishing manufacturing facilities in emerging countries to gain higher market share. Increasing collaborations between academic research institutions and DNA synthesizer providers is also expected to support market expansion over the forecast period.

Market drivers Increasing R&D investments in genomics and biotechnology: Growing investments by governments and private organizations in genomics research, drug discovery and precision medicine is one of the key drivers of the DNA synthesizer market. Synthesized DNA is finding increasing use as research models, biomarkers and targets in genomics research. Advancements in genome engineering techniques relying on gene synthesis is further fueling demand.

Development of new gene and vector synthesis applications: Emerging applications of gene synthesis such as development of gene and vector therapeutics, manufacture of oligonucleotides and molecular tools are propelling growth of DNA synthesizer market. Manufacturers are developing new synthesis chemistries, throughput methods and software to enhance utility in these applications. Need for shorter turnaround times and lower costs in application development is driving automation and scalability of DNA synthesizers.

Growing synthetic biology market: Expansion of global synthetic biology market that relies on use of synthesized DNA as foundational blocks is a major growth driver. Rising tools development, workforce expansion and investments are accelerating application scope of synthetic biology, thus supporting DNA synthesizer adoption. Development of CRISPR-Cas9 technology requiring customized DNA and RNA oligos is positively impacting DNA synthesizer sales.

Impact of geopolitical situation on DNA Synthesizer Market growth and future strategies

The current geopolitical tensions have impacted the DNA Synthesizer Market in multiple ways. Supply chain disruptions caused due to the Russia-Ukraine conflict have created delays and increased the costs of raw materials procurement for market players. Transport restrictions implemented between certain countries have also posed challenges in seamless movement of finished products. Furthermore, heightened political risks and economic uncertainties prevailing across some major markets have reduced expenditure on research activities utilizing DNA synthesis technologies by academic institutions and biotech firms.

To cope with these difficulties in the short-run, manufacturers are focusing on developing alternative regional sources for key components and exploring new logistics routes. In the long-run, building flexible manufacturing footprints spanning multiple geographic clusters would help mitigate regional supply chain dependencies. Forming strategic alliances with local players in emerging economies represents another route to circumvent geopolitical barriers and tap into high-potential growth areas. Additionally, intensifying R&D efforts to introduce more automated and affordable benchtop DNA synthesizers can aid wider adoption even during times of constrained research funding.

Geographical regions with high concentration in DNA Synthesizer Market

North America has historically been the dominant regional market for DNA synthesizers, accounting for over 40% of the global revenue. Presence of a large number of well-established life sciences and biopharma companies engaged in advanced research & development activities has sustained the high demand. Additionally, considerable investments by governments and private entities in synthetic biology and genomics domain have propelled the regional market. Europe is another major revenue generating region, supported by presence of various global industry leaders and a robust academic research infrastructure. Continuous technological improvements by domestic players also contribute to its prominent position.

Fastest growing region in the DNA Synthesizer Market

The Asia Pacific region is poised to witness the fastest expansion in the DNA Synthesizer Market over the coming years. This growth can be attributed to increasing R&D expenditures by pharmaceutical companies to accelerate drug discovery initiatives relying on DNA synthesis. Establishment of global research centers by leading organizations and expanding involvement of domestic players are amplifying regional demand. Additionally, growing focus of governments to strengthen synthetic biology and healthcare research through investments and initiatives offers a promising outlook. China, India and other Southeast Asian countries stand to gain significantly owing to their large population base and growing biotechnology industry.

[reportprimerahul]

Loop-mediated Isothermal Amplification (LAMP) Kit Market Size, Type, segmentation, growth and forecast 2023-2030

Loop-mediated Isothermal Amplification (LAMP) Kit Market

The Loop-mediated Isothermal Amplification (LAMP) Kit Market is expected to grow from USD 93.00 Million in 2022 to USD 164.00 Million by 2030, at a CAGR of 8.40% during the forecast period.

Loop-mediated Isothermal Amplification (LAMP) Kit Market Size

The Loop-mediated Isothermal Amplification (LAMP) Kit is a tool used for the detection and amplification of DNA or RNA segments in various applications such as medical diagnostics, agriculture, and scientific research. The market for LAMP kits is segmented based on type, application, and region, with market players including Eiken Chemical, HiberGene Diagnostic, Nippon Gene, Mast Group, New England Biolabs, Optigene, and Lucigen Corporation. Regulatory and legal factors specific to market conditions are also considered, ensuring compliance with regional laws and standards. The market is expected to see growth due to the increasing demand for accurate and fast diagnosis in medical and agriculture industries. The Asia Pacific region is expected to have the highest market share due to the presence of major market players and a growing need for efficient diagnostic tools.

Loop-mediated Isothermal Amplification (LAMP) Kit Market Key Player

Eiken Chemical

HiberGene Diagnostic

Nippon Gene

Mast Group

New England Biolabs

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Loop-mediated Isothermal Amplification (LAMP) Kit Market Segment Analysis

The Loop-mediated Isothermal Amplification (LAMP) Kit target market is primarily composed of molecular biology and genetics researchers, medical laboratories, veterinary clinics, and drug discovery companies. This technology is widely used in diagnosing infectious diseases, such as HIV, tuberculosis, malaria, and influenza. Moreover, LAMP is used in food safety testing, plant pathology, environmental monitoring, and forensic science. Recently, with the COVID-19 pandemic, there has been a surge in demand for LAMP diagnostics due to its high sensitivity, specificity, and portability.

The major factors driving revenue growth of the Loop-mediated Isothermal Amplification (LAMP) Kit market are increasing prevalence of infectious diseases, rising demand for rapid and accurate diagnostics, technological advancements in LAMP technology, and growing awareness among end-users. Moreover, the ease of use, cost-effectiveness, and user-friendliness of LAMP kits compared to traditional PCR and ELISA-based assays are also contributing to the market growth.

The latest trends followed by Loop-mediated Isothermal Amplification (LAMP) Kit market include the development of automated LAMP platforms, the integration of LAMP technology with microfluidics, and the incorporation of LAMP into point-of-care devices. Additionally, there is a growing trend towards the use of LAMP in field-based and resource-limited settings due to its suitability for use in low-resource settings.

The major challenges faced by the Loop-mediated Isothermal Amplification (LAMP) Kit market are the lack of standardization in LAMP protocols, the need for robust quality control measures, and the limited availability of LAMP assays for specific pathogens. Moreover, there is a need for regulatory approval of LAMP assays, which could lengthen the approval process and increase the cost of LAMP kits.

The main findings of the report highlight the rapid growth of the LAMP market, the increasing adoption of LAMP in various industries, and technological advancements in LAMP technology such as automation, miniaturization, and integration with microfluidics. The report's recommendations include the need for standardization of LAMP protocols, the establishment of quality control measures, the development of more specific and sensitive LAMP assays, and the need for regulatory approval of LAMP assays.

Overall, the Loop-mediated Isothermal Amplification (LAMP) Kit market is expected to continue to grow rapidly owing to its numerous advantages over traditional diagnostic methods. The increasing demand for rapid and accurate diagnostics, especially in the context of emerging infectious diseases, is expected to drive the market growth in the coming years. However, the challenges of standardization and quality control must be taken seriously to ensure the reproducibility and reliability of LAMP assays.

This report covers impact on COVID-19 and Russia-Ukraine wars in detail.

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Market Segmentation (by Application):

Medical Diagnostic

Agriculture

Scientific Research

Information is sourced from www.reportprime.com

[mageshima]

マゲシカに関する新たな発見！

9月8日に発表された論文を何度も読み、遅まきながらやっと理解できたのでシェアします。 論文内「表２」の功績がすごい！ ＊いちばん下にリンクあります。

どういうことかというと、 ・馬毛島と種子島間の個体の移動が制限されていることを示唆する。 ・馬毛島および種子島間の遺伝子流動が長期間制限されていたと考えられる。 （引用：「馬毛島および種子島間のハプロタイプ頻度の～利用可能性」項より）

つまり、馬毛島も種子島も同じマゲシカではあるけれども、固有タイプも共通タイプもいるということ。2島間を泳ぎ渡るマゲシカの噂とか、馬毛島のマゲシカを種子島に連れてきて保護すればいい、などの議論は、2島間のシカが遺伝子レベルで異なることから、一緒くたにしてはいけないということ。

そして重要なことはすべて「マゲシカの保全管理上の提言」項に書いてありました。

繰り返しになりますが、この研究のすごいところは、 “馬毛島と種子島間におけるmtDNAハプロタイプの組成の明確な違いが示された”ことだと思います。

これがどういうことかというと、

●環境省に対しては、 2014年レッドリスト改訂「馬毛島のニホンジカLP」（環境省2014）は、「マゲシカの馬毛島個体群LP」とする方が適切、という科学的根拠を持った提言ができ、

それから、

●防衛省に対しては、 馬毛島の環境アセス記載の“馬毛島の個体群に固有の遺伝子は確認されませんでした”（熊本防衛局 2022）という記述は、mtDNAと対立遺伝子の固有性の観点から、結論が全く変わってしまう。

と言えることにあります。 さらに、在来生息地としては馬毛島にしか存在しない対立遺伝子も存在しうるが、2001年以降、馬毛島における調査が認められない（立澤 2021）とのこと。

プレスリリースでは、そこまで踏み込んで書かれていませんでしたが、掲載論文をきちんと読むと、研究者の情熱が伝わってきてドキドキわくわくしました。ミトコンドリアDNAすごい。科学ってすばらしい！

(論文)マゲシカCervus nippon mageshimaeの遺伝的独自性についての再検討

プレスリリース

[mcz-orz]

ももクロ 15周年ツアー 雑感（ネタバレっていうか妄想全開っすよ）

8月1日追記： 「はじめに」の前のさらにはじめにw 7月末日に公開された玉井さんのAEインタビューで、周年ツアーの演出やスタッフに触れる記述がありました。その内容を汲むとクソ恥ずかしいほどに的外れなコトを書いている部分だらけなんですが、そもそも妄想まとめなので、そこは寛大なココロで生温かく見守ってやっていただけると幸いでございますw ＝＝＝＝＝＝ はじめに ライブの感想っていうより、あのライブを通じて自分が受け取ったモノというか、勝手な妄想ですw セトリ、演出、あと細かいコト、もろもろを全部つないでった結果、自分が感じたのは、このライブって「メンバーからノフに対しての、今後のももクロライブについてのプレゼン」ではないか？ということでした。 ＝＝＝＝＝＝

行ったのは、2公演目となる名古屋公演。 まずはセトリから…ですが、自分の記憶があまりにも曖昧だったので、先に上がっていた複数の方のネタバレ等を参考にさせていただきました←

■周年ツアー セトリ（多分） ・走れ！ ・DNA狂詩曲 ・PUSH ・笑一笑 自己紹介1 ＝ ・another world ・じゃないほう ＝ ・バイバイでさようなら ・Guns N' Diamond ・PLAY！ ・ゴリラパンチ ・Nightmare Before Catharsis（ラケットでボール打ち） 自己紹介2 ・Re:volution ・HAND ・リバイバル ＝ ・あーソロメドレー ・赤い幻夜 ＝ ・天国のでたらめ ・GOUNN ・MONONOFU NIPPON ・いちごいちえ 　（ももパン → 一味同心メドレーを挟み込み） ・行くぜっ！怪盗少女 ＝En＝ ・DECORATION ・吼えろ ＝ ＝＝＝＝＝＝ ということで、ライブを観終わって、つらつらと考えたことを。 最初にもってきたけど、いろいろ思い巡らせて、わりと後になって、頭に浮かんだことが以下になります。

●周年ツアーの意味と役割

15周年という区切りのツアーだけど、おめでとうとこれまでを振り返るよりも、こっからを作るための、実験的な試みを含んだライブという印象。年月を経て、メンバーもノフも歳を取って、これからも一緒に歩んでいくにはどんなライブにしていけばいいか？自分たちが進みやすいやり方にモノノフを導くには？そんなことが裏テーマにあるんだろうなと。 ひとことで言えば…

「サステナブルなももクロライブ」を作り続けるためのライブ

で、それがなぜこのツアーなのか ・15周年という区切りの年だから「変わる」ことも不自然ではない。 ・メンバープロデュースだから、自分たちの意思を明示しやすい（文句も出にくいw） ・複数公演だからいろいろ実験ができるし、繰り返すことで浸透しやすくできるかもしれない。 みたいな感じですかね。 ＝＝＝＝＝＝ プレゼンと感じたのは、今までと違うものをいくつか提示されたように思えたから。以下、それを含めた雑感もろもろです。

●自己紹介変えたい…よね？

自己紹介パートが2回あった。 １回目は、スクリーンに名前が表示され���センターに出てきてポーズ＆ひとこと。 2回目はいつもの自己紹介。 たしかに30越えて今の自己紹介をやり続けるのはキツイ。正直いえば自分はあの呪縛から解放してあげたいとここ数年思ってる。客観的に見て、あの年齢であの自己紹介はイタイっしょ。新たな自己紹介に変えたいが、反応はどうか？どういう自己紹介がいいか？そこを探ってる気がした。なので、ここは千秋楽まで変化する可能性のあるパートだと思う。 いつもの自己紹介もやったのは、一気に変えちゃうとビックリして拒否反応を示すノフもいると思うので、それを避けるためのクッションじゃないかと。ひょっとすると、先日の「あちこちオードリー」がメディア出演でラストの自己紹介、貴重な映像になるかもなぁ…とか思った。

●怪盗少女のカタチ変えたい…のか？

百田さんの腰への負担もあり、落ちサビ前間奏のアクロバットをなくす方への動きなのか、順番にアクロバットするところは、メンバーによる「ハイ、ハイ、ハイ」の煽りに。 一瞬、違和感を感じ、そして「これは、大天使口上打ちづれぇ…」と。目の前で、あーりんが「ハイ、ハイ」煽ってるのブチ破って口上打つ勇気なんかなかったw 結局、ももクロMIXをギリギリ打てただけだった。 でも、これ座席がステージに近過ぎた（実質2列目）から、そうなっただけで、離れてたら違った（普通に全力で大天使口上打ってたw）とも思う。

●生まれ変わりますからね宣言？

第2ブロックのスタートが「バイバイでさようなら」で、ここにちょっと違和感を覚えたんすよ。新しいブロックをなぜ死んじゃう曲からスタートするのかって。で、第3ブロックが「でたらめ」で始まり「GOUNN」につながって、あ…生まれ変わるってことか…と。

「バイバイで」→「ガンズ」（第2ブロック） 死んじゃって

なんやかんやあって

「でたらめ」→「GOUNN」（第3ブロック） 生まれ変わる

の流れは、生まれ変わって新しいものを提示してくからという意思表示じゃないかと。アルバム（Amaranthus）では「ガンズ」「バイバイで」の順だったけど、その順を入れ替えたのは、足掻きとか迷いとか空想とかその後のなんやかんや（「PLAY！」は “Welcome to 次なる世界”だし、「リバイバル」は復活の意味合いを含んでたりしするし…って、ゴリパンを説明できないから、こじつけでしかないんですけどもw）を経て「でたらめ」への道筋を作っているようにも思った。

で、衣装早替えで生まれ変わってからの「GOUNN」。輪廻転生をテーマにした「GOUNN」のフリがまるっと変更されてるのが、このブロックの胆。こんなの唸るしかないべ。そっからのヒャダイン新曲。変えてく、変わってくけど、楽しいことは続けてく���宣言みたいな。 何が言いたいかというと「若かった頃のももクロのやり方とはお別れするけど、生まれ変わっても楽しいモノを届けてくからね」ってメッセージが込められてるんじゃないかと。 なお、その「MONONOFU NIPPON」ですが、めちゃめちゃハードです。たとえば10年後にパフォーマンスするとか想像できないレベルのハードさw。で、楽しい。タイトルやコンセプト、曲調、歌詞、ノリ含めて、世界的にバズらせること狙ってるよな…あ…OVERTUREをバズらせたら、ここにつなぐってなシナリオか…とか思うなどw

●GOUNNのフリの今後

フリを一新された「GOUNN」。あのブロックの構成上、これ以上に適した曲はないことは理解できる。演出としても素晴らしい。でも、GOUNNのフリコピ大好きマンとしてはやっぱり寂しさも感じるわけで。ただ、MCで、大きくフリを変更した曲について、このツアー限定で…みたいなニュアンスのことを言ってたと思うので、そこに望みをつなげたいと思う。

●「いちごいちえ」の演出

「いちごいちえ」の間に過去曲メドレー（2009年「ももいろパンチ」から2022年「一味同心」まで）を挟み込み、過去から今までを辿る構成。今では変わってしまったフリも昔のフリを使ってるところも含めて、この曲自体の演出は、過去も全部未来に持ってくから置き去りにしないから、で、楽しいことを続けてくからね。みたいな気概を感じた。

●メンバーのソロの使い方

メンバーのソロ曲をインタールード的に挟み混んで衣装替えの時間として活用する。これまでもイベント等ではやったことはあるけど、ここまで明確に役割持たせてやるのはなかった気がする。これ、めっちゃアリだと思うし、今後バンバンやってほしい。

●4人でのハモ

「走れ！」「リバイバル」でイントロ・アウトロ等でのハモアレンジ。多分、自分の席が音の環境的に良くなかったのだと思う。ガチャガチャうるさくしか聞こえなかったので、PAさん頑張ってくださいw

●演出について

全体的な演出イメージとしては、歌詞の世界観を大事にしたというか歌詞をパフォーマンスに落とし込んだモノが多い気がして、最近の傾向で考えると、そのあたりは、やっぱりあーりんがイニシアチブ取ってるっぽいって思っちゃうんすよね。

●ももクロにとってのモノノフ

心強い味方である人が些細なことで敵に変わるなんてことはよくあることで、彼女たちはそのこともものすごくわかってると思う。だから、説明もなしに、一気に舵を切るのはリスクが伴う。媚びてはないけど、今回の変わろうとしている中で、まずはノフを納得させる、同意を得る、共犯にする。そういった部分への配慮はめちゃめちゃ感じられる。思えば、これまでもちょっと刺してみて、これくらいなら���丈夫かな。ってリアクションが得られれば、次��もうちょっと攻めてみるか…みたいな感じで、受け入れエリアを広げてきた感はある。間違えたらそのやり方はやめて、次の手で探る。ライブの現場はダイレクトにリアクションが受け取れるし、ツアーだとそれが繰り返しできるから、いろいろ試しやすいよな～とか思ったりもした。 ＝＝＝＝＝＝ あとがき 観たライブで受けた印象から、いろいろ妄想してんの楽しい← 今回のツアー、そもそもセルフプロデュースで何をするか…にいちばん興味があったんすけど、ぶっちゃけ自分は、エンタメとしてどういう構成、表現、手法を持ってくるんだろうと、そっちにしか意識が行ってなかったんすよね。 だから、お恥ずかしいことに、裏テーマに、ももクロのライブをこれからも続けていくにはどうすればいいか…みたいなことがあるとかまったく想像してなくて。その可能性に気づいた時に、やっぱももクロおもしれぇ。って嬉しくなったし、わかった！着いてくから。って気持ちを新たにした感じがありました。っても、自分の勝手な解釈と妄想なだけで、実際はそんな裏テーマなんてないかもしんないけどw ツアー期間が長いので、変わってく部分もあると思うし（あってほしいw）、できたら10月の武蔵野森は行きたいんすけど、チケ取れてないので、なんとか頑張って確保したいと思っております。 ＝＝＝＝＝＝ 私からは以上です。

[helloprovocalsonly]

Nippon no D・N・A！ - BEYOOOOONDS

[tampon-on-the-sidewalk]

Kaden Finally Watches the Beyooooonds MVs:

Okay y’all I’ll put this under a cut bc there’s a lot to take in but this is your boy’s first time actually sitting down and watching an MV since... Honestly, I think Furari Ginza(and before that the last MV I actually paid attention to was Shuukatsu Sensation). I almost entirely gave up on H!P after going to Japan and seeing both Meimi and Zukki’s graduation concerts live minus a few songs catching my interest here and there(and then I forced myself to get back into Momusu when I saw them in New York last year) but I’ve heard so much good about Beyooooond’s debut, not to mention the fact that they even surpassed Momusu with their best first week sales, that I had to watch.

1.Megane Otoko no Ko- ~Okay the talking bit in the beginning was adorable, the rakugo reference and then the girls holding up little manga frames around them like that was super cute. ALSO I swear I know the artist behind the mock manga cover they showed in the beginning

~And then the music hits and BAM we're back into mid 2000s style songs. Gave me a Berryz vibe right from the get go. This is super cute and catchy and I need a dance shot of this XD

~the fuckin rave scene in the bus XD

~the RAP

~This is.. So nostalgic and yet refreshing like I'm definitely intrigued. Let's see how the other two songs match up!

2.Nippon no DNA ~Already I'm loving these outfits

~Another song that immediately slaps you in the face with coolness

~You definitely get more of a feel for everyone's voices in this, more solos and much more power

~This is SUCH A BOP The chorus makes me wanna dance

~"Why do you bow when you're on the phone" ffff

~These music videos are the most fun I've had watching since Country Girls like there's just an exuberance of joy coming from them

~YEEEE ROCK THAT KEYTAR

~I need a colour coded version of this bc there are certain voices that really stand out but I literally have no clue who anyone is

3. Go Waist ~I actually really love that all of these open with a mini story scene like I don't think they've(Hello!Project I mean) done this in a long while

~THIS IS SO FUN It's very reminiscent of another song in melody and I can't place it, I dunno if it's a classical song I'm thinking of(probably) but I know it's not another H!P song I'm thinking of. Honestly it reminds me of a Eurodance song (OKAY YEAH It reminded me of Speed Over Beethoven so both were right XD)

~This is the Rap Group definitely

4. Kaden's final thoughts: ~Nippon no DNA is definitely my favourite of the three, and probably the only one I'd put on my iPod if I still had one, and maybe Megane Otoko no Ko(can anyone who bought the single tell me if the speaking part is part of the legit audio or does it go straight to the music?)

~Go Waist isn't really a casual listening song, I think

~The MVs are well thought out, fun and energetic. Definitely the best MVs I've seen in a long while and Megane Otoko no Ko is my favourite MV since Boogie Woogie LOVE

~All-in-all, this group is definitely a force to be reckoned with and I can’t wait to see what else is in store with them. 

[lovefoundmodelslost]

Model - Miki Ehara

Nationality - Japanese

Height - 5'10

Agencies - DNA (NY) Viva (Paris, London)

Notable Work - Vogue Nippon February 2018 Editorial

[feliciadraws]

A full view of Waka and Mei's kids, human/Moon Tribe hybrid twins Rin and Yumi! I had already drawn them with Nippon's number one babysitter Ammy, but I figured that they needed a proper full view! Being Waka and Mei's children, they're half human, half Moon Tribe, and each inherited a different degree of human/Moon Tribe DNA from each parent.

Rin, the boy on the left, is the more human-looking of the two, and has Mei's physical traits but Waka's cheekier, more outgoing personality, and he's the more energetic, naughtier of the two. Yumi, the girl on the right, is the more Moon Tribe twin, inheriting Waka's physical appearance but has Mei's quieter, more gentle and reserved personality. Because of her blonde hair, Yumi likely has more Moon Tribe blood in her than her brother Rin, and possibly inherited Waka's clairvoyance, too! I have alot of ideas for these two, mainly concerning Ammy babysitting them but also of what they might become when they grow up!

[ukaiknowsbest]

Daiya no A x NPB Collaboration videos found on YT

feat. Ceremonial First Pitches and announcements performed by DnA voice actors.

TOKYO YAKULT SWALLOWS

2016 Swallows Hiroki Touchi (kataoka), Ohsaka ryota (eijun), saito soma (mukai),masuda toshiki(gou) and tatsuhisa suzuki (mishima) doing ceremonial first pitch for the Swallows https://www.youtube.com/watch?v=6gI4bkxwZZ4

2017 yoshitsugu matsuoka (kanemaru), nobunaga shimazaki (furuya), ohsaka ryota (eijun) and hanae(haruichi) ceremonial first pitch for swallows https://youtu.be/AtIMAxLf2x4

2019 uchida yuuma (okumura), ohsaka ryota (eijun), and sakurai takahiro (miyuki) ceremonial first pitch for swallows https://youtu.be/wPzEcFE5PLA

2019 ohsaka ryota, sakurai takahiro and uchida yuuma dancing bon odori song with smol umbrellas for swallows rain break (?) https://youtu.be/0FRNyeqxglk

FUKUOKA SOFTBANK HAWKS

2018 softbank hawks ceremonial first pitch feat. kominato bros (nobuhiko okamoto and hanae natsuki) + sawamura https://youtu.be/8LIWHqFG-zQ

2019 Sakurai, Ohsaka and Nobunaga doing ceremonial first pitch for softbank hawks https://www.youtube.com/watch?v=XDka2wc80Jk

HANSHIN TIGERS

2018 ohsaka, sakurai, kaji yuuki and ono kensho ceremonial first pitch for hanshin tigers collab https://youtu.be/xKtKGrv8NnQ

2021 ohsaka ryota announcing tigers lineup https://www.youtube.com/watch?v=Hb-5SMGp0kU&t=109s

2021 Ohsaka Ryota Ceremonial First Pitch photos https://ukaiknowsbest.tumblr.com/post/664083388010053633/ohsaka-ryota-ceremonial-first-pitch-for-oct-3

2021 Ohsaka first pitch video https://www.facebook.com/100023429795464/posts/999330320857952/?d=n

NIPPON HAM FIGHTERS

2016 ohsaka ryota for nippon ham fighters https://youtu.be/nGT5yhOqMOg

2016 nobunaga shimazaki (furuya) ceremonial first pitch for nippon ham fighters https://youtu.be/QuEuOUrw0mg

2016 shimazaki as announcer for nippon ham fighters game https://youtu.be/wvEzThJf5bs

SAITAMA SEIBU LIONS

2015 aoi shouta (tojo), ohsaka ryota (eijun) masakazu morita (tanba) seibu lions ceremonial pitch for daiya act 1 https://youtu.be/So82scq1Gl0

2015 morita, aoi shouta and ohsaka ryota https://www.youtube.com/watch?v=4TFhIjBTICo

2017 morikubo showtaro (umemiya), ishikawa kaito (shunshin) and ohsaka ryota pitch for seibu lions https://youtu.be/nAHp3XzQamQ

YOMIURI GIANTS

2016 yomiuri giants ft. daiya seiyuus Tomoaki Maeno (Harada), Kaji Yuuki (Mei) Ohsaka Ryota (Eijun), Sakurai Takahiro (Miyuki), Ono Kensho (Raichi) https://youtu.be/koAuiJod1CA

CHUNICHI DRAGONS

2015 here's the ceremonial first pitch with shimono, sakurai and ohsaka https://youtu.be/-KNciH28hSs

2015 sakurai takahiro and shimono hiro for swallows x dragons game interlude https://youtu.be/ykhp8Lu8PHE

2015 Ohsaka Ryota shooting merch to the crowd while shimono announces stuff 📷 https://youtu.be/9H1Ut5YEAm8

TOHOKU RAKUTEN GOLDEN EAGLES

2016 ohsaka ryota, asanuma shintaro and hanae natsuki announcing the starting lineups https://youtu.be/6Tx3cjIKIEE

2017 asanuma (kuramochi), shimazaki nobunaga (furuya), ohsaka ryota (eijun) announcing rakuten eagles merch https://youtu.be/m6KTBt1Oic

2017 asanuma, shimazaki, ohsaka introduction and ceremonial first pitch https://youtu.be/5gcJ2dswMWQ

bonus

2016 Ohsaka Ryota performing ceremonial first pitch for Japan vs USA softball game in Tokyo Dome https://www.youtube.com/watch?v=YPX9WFQCkKM (ohsaka ryota used to be softball player in highschool)

[lupinepublishers]

Lupine Publishers| Association of Prx Gene Expression with the Gingival Phenotype.

Keywords

Gingival phenotype; Prx; Periodontal surgery; Transcription factor

Introduction

In addition to their role in development Prx genes have important roles in cell proliferation and migration [1]. Smooth muscle cells transfected with rat Prx1 showed increased proliferation [1]. Moreover, treatment of fibroblasts in vitro with morpholino oligos designed to specifically inhibit Prx1 and Prx2 protein expression resulted in downregulation of Tenascin-C expression and reduction in cell migration towards fibronectin [1]. Specifically, the healing process after surgical manipulation in the oral mucosa, is determined in a high degree by the gingival phenotype. Assuming that the gingival phenotype might be regulated by gene expression, the aim of our study was to investigate if there is an association between the presence and the levels of Prx1 and Prx2 mRNA and the phenotype of human gingivae.

Materials and Methods

Subjects included in the study: Twenty two patients in need of crown lengthening were included in the study, ten with thick and twelve with thin gingival phenotype. The assessment of the gingival phenotype was done based on the clinical characteristics of the gingiva. Surgical Procedures: All surgeries were performed by postgraduate students at the Department of Periodontology and Biology of Dental Implants at the Aristotle University of Thessaloniki. All patients were required to sign a consent form before. Tissue samples: Connective tissue fragments 1mm x 1mm were collected from the buccal flap during the surgical procedure and immediately placed in sterile tubes containing 1mL of RNA later (Sigma). Samples were maintained in RNA later at -80°C until further processing. RNA isolation-Reverse Transcription: Total RNA was isolated from tissue samples using the NucleoSpin RNA Kit (Macherey Nagel) following the manufacturer’s protocol. The concentration and quality of RNA in each sample was determined using a SmartSpec Plus spectrophotometer (Biorad). 200ng of RNA were reversed transcribed to cDNA using the PrimeScript II 1st strand cDNA synthesis kit (Takara) using oligo-dT primer. cDNA samples were stored at -20°C until further processing.

PCR Analysis for Prx1 and Prx2 mRNA Expression: The presence and levels of Prx1 and Prx2 mRNA expression were examined by semiquantitative PCR analysis. Two microliters of the cDNA were directly used as a template for the PCR in a total reaction volume of 25μL. The reaction mixture contained 12.5μL OneTaq Hot Start 2x Master Mix with standard buffer (New England Biolabs), 1μL of each primer (10μM), 2μL cDNA template, and PCR grade water. For detecting the presence of Prx1 primers Prx1F 5’-TCCCTCCTCAAATCCTAC-3’ and Prx1R 5’-ACTATATTCCTTGGCCTTC-3’ [2] were used.

For Prx2 detection primers Prx2F 5’-AGCACAGTGCCACCCTACA-3’ and Prx2R 5’-CTTGGCAGGCTCTTCCACC-3’ [2]. Expression of Gadph was used as an endogenous control. The primers for Gadph detection were: Forward 5’-TGG TAT CGT GGA AGG ACT CATGAC-3’ and Reverse: 5′-ATG CCA GTG AGC TTC CCGTTC AG-3′ [3].

PCR conditions were as follows: initial denaturation for 5 minutes at 94°C, then 25 cycles of denaturation at 94°C for 30s, annealing at 48°C for Prx1, 52°C for Prx2 and 48°C for Gadph for 30s, extension for 45s at 68°C and final extension at 68°C for 5 min. PCR products were visualized in a 2% agarose gel using Midori green Advance (Nippon Genetics) DNA/RNA stain and a FastGene LED Transilluminator (Nippon). Semi-quantification of the PCR products was done by using the ImageJ (https://imagej.nih.gov/) analysis program. All experiments were repeated twice to ensure statistical accuracy.

Statistical analysis: The differences between thin and thick phenotypes were analyzed using t-test. Inter-group differences were analyzed by t-test and Mann-Whitney test for independent samples. A p- value <0.05 was considered statistically significant.

Results

PCR Analysis for Prx1 mRNA Expression: PCR analysis showed Prx1 mRNA expression in higher levels in thick gingival phenotypes compared to thin. In addition, both Prx1a and Prx1b alternatively spliced isoforms were detected and Prx1a was more abundant in most of the samples compared to Prx1b. In thin gingival phenotypes Prx1a was either expressed in different levels or completely absent, fact that indicates that thin gingival phenotype might be regulated by other genes’ expression (Figure 1).

PCR Analysis for Prx2 mRNA Expression: PCR analysis showed that Prx2 mRNA expression was absent in thick gingival phenotypes except for one sample. On the contrary, Prx2 was highly expressed in all samples with thin gingival phenotype (Figure 2).

Discussion

Recent studies determine the gingival phenotype based only on clinical characteristics of the gingiva. Placing the periodontal probe in the gingival sulcus can give the appearance of transparency of the gingiva and this biotype is characterized as ‘thin’. If the gingiva is not transparent when placing the probe, the gingiva is characterized as ‘thick’ [4,5]. In this study we aimed to associate the expression of Prx genes with the gingival phenotype. Our results indicate that Prx2 is expressed in thin phenotypes, whereas Prx1 is expressed in thick phenotypes. It is well established that Prx genes play an important role in craniofacial development. Our study indicates that Prx genes are not only expressed in tissues of ectomesenchymal origin, but they might also play an important role in determining the gingival phenotype at a molecular level. It is interesting t that Prx2 was almost exclusively expressed in thin phenotypes and in the same samples Prx1 was absent, implying opposing roles of the two genes in adult gingival tissues. Overall, our study indicates a strong association of Prx1 and Prx2 gene expression with the gingival phenotype. It is possible that Prx gene expression can be used as a molecular screening test to define more accurately the gingival phenotype.

Acknowledgment

Apostolos S. Angelidis, D.V.M., M.P.V.M., Ph.D. Laboratory of Milk Hygiene and Technology Department of Food Hygiene and Technology School of Veterinary Medicine Aristotle University of Thessaloniki, Greece

[archertechno]

Fluid Room 07/10/2021 EVERY Friday www.clubradio.one/ START 9pm (it) Track list. 1-Moby - Hymn (European Mix) 2-VoMar - Back To The Moon (Club Mix) 3-Vicious - Listen To Them (Original Mix) 4-EJE - New DNA (Original Mix) 5-Klankwerken - Nippon (Space Jockey Remix) 6-Pitch - Back to the Rave (Original Mix) 7-Freak Unique-Cocain (Original Mix) 8-N.O.B.A., DOLBY D., Shadym-Attention 2 (I AM BAM Remix) 9-Play Insane - Integrated (Original Mix)