#my beautiful little gram-negative bacilli

[radioproto]

I LOVE SCIENCE- did I ever mention that???

I am so proud of my little bacteria. I grew these from scratch; I got the sample, streaked it on an agar plate, grew them over the weekend, transferred them to a slide, stained them, and they turned out so beautifully on my microscope •v•

[dogtorari]

YALL LOOK AT MY “UNKOWN” I FOUND TODAY IN THE LAB! PLEASE SWIPE RIGHT AND READ FOR THE EXPLANATION BELOW!!!

It was E. Coli and on my EMB Agar plate it showed this BEAUTIFUL green color! 🦠 🧫❤️🥼 🧪

EMB Agar plates are a selective medium because the dyes will inhibit growth of many gram-positive bacteria! This plate showed me bacterial colonies! This plate is used mainly to identify enteric bacilli (bacteria that inhabits and infects the intestinal tract). The green metallic sheen color shows us how acidic our bacteria is! Isn’t this cool!?!!

The next picture shows my other plates which are TSA (general medium plate which is clear at first but can grow BOTH bacteria and fungi)

MAC (MacConkey Agar plate is used to process specimens that contain or are contaminated with fecal matter).💩❤️ MacConkey Agar is selective because gram positive bacteria are inhibited by crystal violet and bile salts included in the medium but gram negative bacteria are not affected. Lactose is added to the medium to make it differential. Lactose- fermenting bacteria are distinguished from non-lactose fermenters by the color of their colonies. My results showed a pink-purple color due to the bacteria utilizing lactose and producing acids to show that color.

& XLD ( XLD is a selective differential medium for the isolation of Gram-negative enteric pathogens from fecal specimens and other clinical material. It is especially suitable for the isolation of Shigella and Salmonella species. Microbiological testing of foods, water and dairy products - my results were yellow colonies which were positive for e. Coli) 🦠 🧫❤️🥼 🧪

I also had a Urea broth which showed little to no change (perfect results for e. Coli as they won’t really grow in this urea broth) 🫶🏽

I hope you enjoy ! This was our last lab for the semester 😭😭 I can’t wait to take another class with my professor, she’s amazing!