#ml ivan chapter 5

[sorcerystudios-writing]

Cleaning Messes: Of Parents and Secrets

by Nakira Taisho

Date Posted: (2020-02-07)

Chapters: 5/10

Fandom: Miraculous Ladybug

Rating: General Audiences

Warnings: No Archive Warnings Apply

Relationships: Marinette Dupain-Cheng | Ladybug/Kagami Tsurugi

Characters: Marinette Dupain-Cheng | Ladybug

Additional Tags: Guardian!Marinette, Fox!Luka, Turtle!Ivan, Bee!Juleka, Post-Episode: s03 Miracle Queen (The Battle of the Miraculous Part 2), Canon Compliant, Chat will be fired, Salt, ml salt, Slow Burn, Cat!Kagami, unwarranted comments WILL be deleted

Summary: Marinette gets Kitty Section some time with Jagged Stone

[incorrectml]

”AGAIN”???

[urbvnny]

"Did you rip your dress in the woods, my beauty?"

(at Ivan. AT IVAN LOL)

"Breathe, girl! It wasn't you who just ran 20 kilometers! Personally, I'd have another ideas to make you sweat..."

My tip to Eloise: grab him and don't let go cuz from there is all a WILD ride with Ivan

FFS THIS ONE

1st: THE SIZE DIFFERENCE! (Apparently Eloise is 1,65, Ivan is 1,78 and Aaron is 1,88. I'm 1,5 cm taller than Eloise, so I think I'd feel too small lol)

2nd: this man straight up says

"You'll get inside Moondance when I give you the sign and will stop people from coming out. I HOPE NOBODY WILL SEE US."

LOOK AT IVAN! He's all red and swollen lol who's NOT going to see if you said you didn't want to hipnotize anyone you didn't need to which is EVERYONE but the victim? That's my man y'all.

Edit: 3rd: his madman face after the plan was all set lol he's the only one smiling

[animegenork]

Thoughts on Ivan Chapter 5

And so, here we are. I’m finally doing this.

As a disclaimer: I’m very very very very very very late, and I know that. But I also needed to finish the chapter before having official thoughts on it. (Because that makes sense.)

And now, I can.

I made a Google Doc to write down my reactions each day I played, as I am free 2 play and needed to keep track of this stuff. So here is the entirety of the mess that is my reacting to this chapter. (I’m putting all this under the cut so those who don’t care don’t gotta deal with this.)

TL;DR: Eloise and Ivan both fucked up. Eloise didn’t trust Ivan enough and ended up breaking Ivan’s trust. Now they’re at a bit of a stalemate because valid feeling vs. valid feeling = hella heated argument. [You’ll have to actually read to get the full story behind this.]

There we are. This was about 11 pages in Google Doc form. I had a lot of thoughts about this chapter.

I’m probably going to reference a couple things from this post I did where I dove into Ivan’s head a little bit. If you want to know more, take a read. I, uh... I ended up being really accurate, and I’m still freaked out.

To be clear, I bounced back and forth throughout this chapter on how I felt about Eloise and Ivan’s actions and words. And let me make a defining statement about my stance:

Eloise and Ivan both fucked up.

They did. Don’t try to convince me otherwise.

Eloise

I made a post once wondering why people were mad about Eloise being jealous. Because let’s face it: jealousy exists. I can tell you a story of a coworker of mine whose ex-girlfriend would consistently harangue him about our other coworker simply because she existed in the same room as him. Jealousy is some powerful shit.

Fans of otome games may not like it or want to deal with the past lovers/jealousy trope, but honestly it’s necessary. Romance ain’t a perfect science, and the more obstacles you have to go through to be with the one you love, the better it’ll be. But this post isn’t about that.

I understand Eloise a lot here. She’s met this Constance, who meant the WORLD to Ivan, and she knows how (seemingly) perfect she is. HELL, I WOULD BE INSECURE TOO. Eloise is insecure; she wonders if perhaps she can ever measure up to this past lover that Ivan had. She’s starting to experience feelings she may not quite understand, and that’s perfectly okay.

However, she kinda went about it all wrong.

Okay, sure. If I heard about my crush going and seeing his past lover during the day (risking life and limb to do so), I’d be a little suspicious. Maybe the “stalker” or “obsessed” thought would cross my mind. BUT if I remembered that Ivan was clinging to humanity AND realized that letting someone go who means the world to you is incredibly difficult, I would probably be more rational than Eloise. Honestly, Ivan’s big mistake here is letting Eloise find out about this outings because they poisoned her mind a bit.

Now, she could have asked him, “Okay, but why follow her?” Because that would have made more sense than doing the exact thing she was (mentally) calling him out for. But no. She did the same thing. And she found she was unable to hate this woman who, now remarried, used to completely hold Ivan’s heart.

What really ticks me off is that she did this because she didn’t believe him when he said he doesn’t feel the same way about Constance as he used to. She didn’t trust him enough to take him at his word. I mean, he’s a vampire, so I guess fully trusting him is off the table, even though she was fully admiring his shirtless form like 6 seconds ago (I mean, so was I), but anyway.

In the end, Ivan gets mad (UNDERSTANDABLY), and they argue because Eloise has somehow become a little obsessed with this Constance idea. Do you remember what I said about my coworker? His ex was ALSO obsessed with the idea of someone else in his life. It’s a pretty valid concern, and honestly, considering the way I argue with people, I don’t think Eloise’s freak out during the argument is completely unjustified. When you’re insecure, you are trying your DAMNEDEST to prove that there is merit to your worries.

I really sympathized with Eloise at the end of the chapter. She was convinced she’d ruined whatever it is she has with Ivan and that she should’ve kept her big mouth shut. This is exactly how I feel at the end of really bad arguments.

Poor Eloise. She’s never been in a relationship, right? So of course things would go wrong in the first one. But that doesn’t mean she needs to lose hope!

Ivan

I might get rant-y here. Only because some things people have said have PISSED. ME. OFF. Especially in relation to what I now know. (I’m not about to address the claim that Ivan needs a straitjacket now, but it might come in the future, if my newly-acquired knowledge of the definition of psychopathy has anything to say about it.)

Okay, so. Ivan definitely could have been a little clearer when he explained his relationship with Constance. He certainly could have clarified his exact reasons for going to the Village during the day. But with the bits and pieces I gathered, it was... I don’t know, obvious? When you care for someone as much as Ivan cared for Constance, you’re going to wonder how they’re faring after your sudden disappearance. It’s a thing in fiction where if X Character disappears, they wonder how life back home is going.

That might just be me over analyzing character motivations again. Sorry. (It’s an English/Creative Writing major thing.)

Still, this boy trusted that Eloise would give him space after he dug into this painful wound of his. He trusted that she wouldn’t ask anymore until he was ready to go back to poking it. But instead, she went and investigated on her own. Not only does that speak of her not trusting him, but now, how can he trust her when she did this?

I wanna note that before shit hit the fan (i.e. the argument), Ivan was perfectly content with Eloise visiting him. He was ecstatic that she would come to him so early in the night. When she gave him the plant, he BEAMED! He told her that he didn’t think he deserved to care for something like a plant! HE TOLD HER THAT THE FACT THAT SHE GAVE IT TO HIM MADE IT MORE BEAUTIFUL!

Have I mentioned I was sobbing at this part?

I’m not about to go into the nuances of the Chalice-vampire bond, because not much has been explained about it. However, Eloise did, in fact, say something that was very like Constance (all flower-knowledgeable and such), and the fact that that BRIEF tidbit clued her in to Eloise’s actions is interesting. So, yeah. He gets mad that Eloise went to see Constance in person, because that is, in fact, an invasion of his privacy in many ways. It’s like if I went to see my boyfriend’s ex in person just because he gave me a name. (I don’t have a boyfriend, and I wouldn’t do that. Just by the way.) But before that...

You know what I didn’t see in people’s reactions to Chapter 5? How worried Ivan was about us. He saw that Eloise wasn’t acting like herself. And he asked THREE TIMES (count ‘em in my reactions above, he asked THREE TIMES) if she was okay. The THIRD time, he said, “Did I do something wrong?”

I want someone to explain to me how in the fucking world this clues us in to him not caring about us. But anyway.

This concern, in fact, comes up when Ivan starts yelling at Eloise about being reckless. Because she was. Going too far from Ivan fucks with the Chalice bond, AND she did it during the day (and nearly got burnt real bad), AND there’s a murderer on the loose, AND Vlad could’ve found out. What I think a lot of people looked past was that there was concern behind Ivan’s anger. Anger based on concern is a helluva drug, and Ivan was high on that.

So, Eloise broke Ivan’s trust and endangered herself while doing it. And people are mad at Ivan? Okay. Cool. Good to know.

You know what was really crazy about all of this? A lot of the things Ivan said in this chapter really resonated with the post I linked above. I didn’t actually know Ivan didn’t take change (which had fucked him over in the past) well, but he directly said so. I didn’t actually know for sure that Ivan saw Constance as the embodiment of what he’d lost when he turned vamp, BUT HE ACTUALLY SAID IT (the part labeled, “OH MY GOD. OH. MY. GOD.”). I only vaguely understood that Ivan had a hard time coping with his transformation and feared that he was gonna ruin his relationship with Eloise. And yet! I ended up hitting the nail right on the head! I can’t tell if this means I’m magical or that I’m too good at diving into character’s heads.

The best part is that Ivan is the one to call an end to the shouting match. I’m kinda glad he kissed her, first of all, because there were some logical fallacies and circular arguing going on PLUS all of the emotions both were feeling PLUS the Chalice bond fucking them up a bit, SO it kinda served to tell him, “We gotta stop.” He basically says (and this is a paraphrase), “Okay, you go chill, and I’ll stay here and chill, because we’re both very angry and should probably calm down.” RATIONAL IVAN.

I’m really, REALLY confused why people are bashing him left and right (and I WOULD bring up the straitjacket thing again BUT).

[Side Note: I think at the beginning of Chapter 6, he ended up going out anyway because after an argument like THAT, how do you NOT get stuck in your own head and need to leave?]

Final Thoughts

This could all be bullshit. Even the parts where I directly quote what the English version of this chapter gave me. I could be going in too deep for the sake of defending Ivan. But what the Moonlight Lovers fandom needs to remember is that nobody ever said Ivan’s route was going to be easy. I got my fluff. I got some damn good fluff in this chapter, too. I NEVER expected things to NOT go to shit for the sake of the overarching plot. I expected it. That’s the point of this game, for God’s sake: things go to shit because of [PLOT].

There are two main things I think some people may have forgotten.

1. Eloise is allowed to be jealous. I’m sorry the past-lover-jealousy trope isn’t everybody’s cup of tea, but I, personally, find it realistic. Maybe she went about it in the wrong way (although we got some hefty dramarama), but I empathize. I’m hecka insecure; I would be acting very similarly to her.

2. Ivan does, in fact, have a point. He has multiple, actually. I don’t blame him for going further into the “so I would go out to see her-” thing, because how does one properly explain something like that to someone who doesn’t know them that well?!?! Plus, his concern is very clear throughout this chapter, so treating him like he has no emotions/doesn’t give a damn about Eloise is pretty fucking stupid. Perhaps he’s acting rather selfishly most of the time, but do you think Vlad and Bel were entirely selfless 24/7? HELL, I’D BE SELFISH TOO. LOOK HOW FUCKED UP HIS LIFE HAS GOTTEN SINCE HE BECAME A VAMP.

I didn’t really ever plan on doing this post, not until I saw people giving Ivan a lot of shit. Frankly, I’m giving him some shit as well. Considering how close in age Eloise and Ivan are, I’m really not surprised shit hit the fan in this chapter, especially with the decisions both of them made.

But then, I still enjoyed it.

So, yeah. I actually enjoyed this chapter. I was tossed back and forth between empathizing with Eloise and Ivan so much that my conclusion - that both of them had a point and both of them had valid feelings and reactions - was not one I thought I’d make when I went in.

And if anybody else actually happened to find merit in this chapter, don’t be afraid to say so. Hell, you can DM me and tell me EXACTLY why you found merit with it. I don’t mind.

Final Final Thoughts

This is a stupid random thought, but. When did Eloise and Ivan become parts of my psyche incarnate??? Like I empathized with them WAYYYYYY too much. Was this chapter written specifically for me? For a real person who’s felt many of these things before?

Beemoov, did you specifically write Ivan’s route for me? As flattered as I am, this is kinda weird. I didn’t expect to see myself so much in the MC of this game, but here we are.

I’m just bullshitting please no one roast me for having a huge ego it’s just that the coincidence was too uncanny

[isia]

Ivan chapter 5

[tetrakys]

The testing team

A while ago I wrote a list of all the people who contribute to the making of the guides in the description of the guides blog, but I reckon I never made a proper post here to introduce them. I’m doing it now to show them how much I appreciate them and so you all know who is involved and you can send them some love if you want to (especially because ML is ending probably in a couple of months and we are considering making Eldarya guides afterwards).

I’m going to introduce the current team, there were a couple of extra people involved in the past who were Ivan stans but left around the middle/end of his route and don’t want to be involved with the game anymore.

So, in the Raphael stans department, we have @incorrectml and @amaaaaterasu who are also Leiftan and Lysander stans, they do like nice, slightly shady gentlemen, don’t they? 😌 They are both really funny (the comedic relief of the group...? they make me laugh a lot 🤭), you can contact the first in spanish and Ama in french. Rinsyo (incorrectml) tests but doesn’t save her games with the guide because she’s a faithful woman of strong principles and aims to get the good ending with Raphael alone. Ama has replayed Ivan’s chapters I don’t know how many times so she probably lost some braincells in the process, be kind to her she is the hero we don’t deserve 🙏

In the Ethan stans department we find @kanraandchrome and @crownysworldofficial who are also Lance stans 🥰 they like problematic, psychos, I wonder what it feels like 😌 Chrome is also a fan of Kentin and Evan, and Crown is a Castiel girl. Crown is the sweetest cutiepie, she always replays the episodes multiple times to be sure to save her route following the guide to the T, she makes sure people can’t miss illustrations so she deserves lots and lots of love. Chrome is our nerd 🤭🤭 if you have a question about lore in general or more specifically Ethan lore (of whom he is the messiah) he is your guy 🤓 he’s also super organised and keeps us all afloat. You can contact them both in french.

Now, I’m not exactly sure where to place @neilada-d6-meghah at the moment, she used to be an Ivan stan and has been mourning the flop of his route ever since chapter 5. Meg, who is your bias at the moment, Beliath? Aaron? Both? She is also a Castiel (I think) and Lance girl 😌 Meg is the nicest person among us all and very diplomatic so she has the very important role of dealing with problems that may arise (especially on FB), she has lots and lots of patience. Also she is the one who communicates with the spanish-speaking fandom which is probably the largest in this game, so if you need to ask a question in spanish contact her.

@cintanna-rants is a new addition to our team, she recognised the Valkyon inside Aaron so she stans and has been playing his route, but I have the long-term plan to throw her into Beliath’s and Ethan’s arms too, don’t tell her 🤫. She is also a Castiel and Lance stan 😌 I have been accused (by myself) to have purposefully filled this testing team with Lance lovers 🤨 this is ridiculous, not my fault if there are lots of dragon f*ckers around 😌 You can contact Cintanna in spanish.

@mcl-pauly is our graphics expert, she is the one who makes the guides in their final format and is also responsible for the nice layout of the guides tumblr blog. Although reluctant at first, she gave Beliath’s route a go because someone convinced her when everybody else still hated him (I will never stop taking the credit for this 😌) and now she’s a rabid Beliath stan. She is also twice widower because of Armin (🥺) and Ezarel, but she’s not-so-secretly-anymore a dragon f*cker so she’s fine. Because all her husbands die I’m trying to revoke her dragon f*cker card and make her stan the new Mathieu guy. Pauly and Chrome are also our snoops, they expertly look into the code and find news and new additions. You can contact Pauly in french.

And finally there’s me, my role is to test and usually to collate all our testing into a first draft of a guide which is then tested and tested again by all of us multiple times to make sure that it works and it’s the best possible version before it’s released to the public. They refer to me as the CEO seemingly because I’m the one who started all this, but I believe this is a nice way to say that I’m a hoe because I stan everyone 🤨 (Ah and yes I’m strongly advocating for a Neil route 😌 I’d say you can contact me in Italian, but I could probably count the number of italian players on one hand.)

Thank you guys for jumping into this expensive, insane and time consuming experience, it’s hard work but it’s fun work because of you 😘

[maggies-scribblings]

Chapters: 3/11 Fandom: Miraculous Ladybug Rating: Explicit Warnings: Graphic Depictions Of Violence Relationships: Adrien Agreste | Chat Noir/Marinette Dupain-Cheng | Ladybug Characters: Marinette Dupain-Cheng | Ladybug, Adrien Agreste | Chat Noir, Sabrina Raincomprix, Xavier Ramier, Nino Lahiffe, Lê Chiến Kim, Max Kanté, Alya Césaire, Ivan Bruel, Chloé Bourgeois, Gabriel Agreste | Papillon | Hawk Moth Additional Tags: Alternate Universe - No Powers, ALternate universe - Witness Protection Programme, Alternate Universe - Police, Gabriel Agreste's A+ Parenting, Slow Burn, Romance, Fluff, Angst, Shooting Guns, Sniper - Freeform, descriptions of blood and injuries, Reference to Surgery, Hospitals, (Unrelated to sex), Smut, Plot With Porn, Explicit Sexual Content, Cunnilingus, Aged-Up Character(s), adrienette - Freeform, Adrinette | Adrien Agreste/Marinette Dupain-Cheng, ML Adrienette NSFW Zine Summary:

Marinette is a young and ambitious police officer. In her first high-profile case, Gabriel Agreste is arrested, with the cooperation of his son, Adrien Agreste. Now that he’s the star witness and a target, the best officers are assigned to protect him. Can Marinette guard Adrien and her heart at the same time? Can Adrien survive endless weeks of isolation? Is Kim a goof? Read on to find out!

(Alternate universe - Witness Protection Programme)

Part of "In the Sheets — A Naughty Adrienette Fanzine".

NOTE: The naughty bits only start on Chapter 5, so if you don’t like NSFW, you can read up to Chapter 4. Let me know if you want to read an SFW version.

@miraculousfanworks​

[mcl-pauly]

What are your takes and feelings about each Vampire and Eloise so far Pauly ? Sorry is someone asked this already :3

Oh I’ve never shared my opinions about ML in details before haven’t I?

I like Eloise! I find her hilarious and I like that she stands up for herself. Most of her reactions makes sense to me from what I can remember right now, the most annoying thing about her that I can think of is when she’s being jealous of another female character, because it doesn’t feel nice to play a character that’s insecure, but so far this is the only thing that bothers me about her character. And I actually like the way she’s dressed lolol wish they had kept her hair black though.

Vladimir was at the bottom of my ranking when the game was released... and he stayed there once I finished his route. I got sick of his mood swings by chapter 5 I think? He has his cute moments but him and Eloise are always arguing in his route. She is also always the one making efforts to make him come out of his shell, then 10 seconds later he’d become cold again.. it was just tiring. His neutral ending actually kind of redeemed him for me (not that I wanted him to die lol) because... I... like tragic characters I guess? I dunno, it kinda made me wanna go back to try and understand his character a bit more, at a moment where I was just sick of him. I wish they had focused on his depression more.

I wanted to drop Beliath’s route when I finished his first chapter. I was uncomfortable (I swear, who was it at Beemoov who decided the Crush would assault Eloise in the first chapter) and I had to spoil myself (which I hadn’t planned on doing when I started the game) up to chapter 3 to motivate myself to continue. And it was the BEST DECISION EVER. By the end of chapter 5, Beliath became my fav vampire. The Don Juan act can be off-putting in the beginning but I SWEAR it gets 100% better the more you progress into his route. His major default is that he’s a bit secretive but once his secret his revealed, it’s just... amazing. He’s so nice and playful, he’s respectful of Eloise and considers her as his equal. It’s the only route so far where the Crush acknowledges that the Chalice bond sucks (when on the other hand, Vladimir says that he “owns” Eloise up until chapter 9)... He’s just really good. I love him. And he’s also so nice when you’re not on his route. When Beliath says that he’s the best choice... maybe you should trust him.

When they revealed Ivan’s portrait back when the game was in development I thought he would be one of my fav... but when the game actually came out... well... let’s say that he wasn’t one. It’s not even because of the fact that he’s responsible for Eloise’s death... I don’t know, I guess the chemicals in my brain changed by the time the game came out lol. Anyway, he was kinda in the middle in my ranking, but he dropped hard once I started his route. He has his cute moments just like Vladimir, but he is... really bad-tempered. His relationship with Eloise is not healthy (they argue a lot too) and maybe it’ll get better but we’re already at chapter 6 and I don’t see any signs of the plot actually addressing the problems they had in the first chapters. I don’t like this weird dead lover trope they’re using for his route, Eloise is always either blaming herself or Constance for Ivan’s behavior and it’s just URGH girl, drop him and go get dicked down by Beliath already would you now. I’m actually enjoying his character more if I just ignore the romance parts (yeah, I’m invested in the Constance-Loic-Ivan drama, sue me).

I don’t really have much to say about Aaron right now. He’s in the middle in my ranking and I actually got more interested in his character after the game came out. He’s been nice so far and he can be really playful I think. I hope his bluntness will help us avoid spending entire chapters on secrets and stuff. Oh and I hope his route will give us lotssss of lore, considering Aaron is the oldest vampire in the manor.

Raphael is really nice and kind. Unfortunately, I have this very specific brain rot that makes me unable to like decent fictional men. Bottom of my ranking you go. It doesn’t mean that I’m not interested in his character though, I actually find it hilarious that he’ll gladly answer Eloise if she asks him about personal infos on the other vampires, but flatly refuse to say anything about himself if Eloise dares to ask. What are you so mysterious for, Jester boy. If ur kindness was genuine you wouldn’t be hiding things from me, Jester boy. Maybe if I bully him enough, I’ll love him by the time his route is released.

Ethan is an asshole. Unfortunately, I have this very specific brain rot that makes me like fictional bad boyz. It’s noticeable that he cares despite the layers and layers of snarks and I think that’s sweet. Now, I adore him in Beliath’s route, but I despise him in Ivan’s. I hate the way he’s behaving in it or the way he’s talking to Eloise, it just goes too far! In Bel’s route, he’s provocative but playful but in Ivan’s he’s straight up unsufferable. On another note, he’s also a character that will flatly refuse to say anything about himself if Eloise dares to ask so I can’t wait to learn more about his past.

[kanraandchrome]

When there’s a double chapter of ML tomorrow (Ivan 8 at 11AM and Aaron 5 at 2PM) and you’re part of the testing team.

[faireth-reed]

Comparing writing styles for the routes: Moonlight Lovers

Before I begin, this’ll be a long post. I just wanted to make Raphael vs Ivan at the beginning, but there’s much more to say. I know that I can’t fully decide my preferred writing style without the 2nd half of the routes, so I’ll take that into account. Careful, there might be some unpopular opinions, and spoilers, of course.

Let’s talk about each of them, by route order. At first I expected to have Aaron and Ethan as my favourites because of my Ezarel-Valkyon love, but these guys are not copies of Eldarya’s characters, so...

Vladimir

The first one tends to have it more difficult. While he has this “main LI” aura, we had to discover all the lore of ML through him, so it meant going at a slower pace. Good backstory, both for Eloise and him, and I was surprised to see a more unique past for Vlad. While some of us felt a bit frustrated at first because of “Vlad is mad at us again”, I really enjoyed seeing his character evolution, how he opened himself up.

Bad points? The route might seem “less unique” because it took a lot of time to explain the main story, and Vlad is not really present in other routes. That’s supposed to change in Raphael’s route, but it won’t be his main writer, so we don’t know how that’ll be.

Beliath

Who doesn’t look at him and say “my man!”? It’s impressive how we expected to get a boring casanova cliché and he was so much more, he managed to make us love him in all routes. And the relationship evolution was a gulp of fresh air, more interesting and a lot of respect. Beliath’s relationship with every manor member is unique, there’s fun and camaraderie.

The only thing I’d change about his route is the exaggeration of Leandra. Even if she’s a quite good character, there’s still that “Chiino’s too perfect/seductive self-insert” vibe.

Ivan

I’ve been thinking a lot of him, because he’s given me this “little bro must protect” ideas. Many people had Aaron-like expectations for him, but the events made lots of them stop playing, and I understand it. But I’ve come to a conclusion about his route.

I used to find his route really contradicting. Half of it gave me the sensation that our Chalice was falling in love out of pity, and that all the vampires were acting too badly, even out of character. But even if we mostly see her thoughts, we are seeing the story from Ivan’s point of view.

Why the clown-wholesome dissonance in every chapter? Is it contradictory or not? Turns out, it’s just two sides of the same coin. We’ve got a young man who’s ashamed of his past (rich boy who partied too much), who’s lived a trauma as big as the Chalice’s, and he doesn’t even remember it properly.

He has this pain that he doesn’t really understand, and he has to mature for his survival, and he’s responsible of other people too. What we see every chapter, even if it makes us uncomfortable, is the amount of reasons that we have to get the good or bad ending. Ivan is really fighting to overcome his condition, but the choices are so crucial that the outcome is either very romantic, or catastrophic.

The cruelty from the beginning is his point of view, he really thinks the others don’t trust him at all. He starts getting healthier when people show him their trust. He’s in desperate need of comfort (and that is worrisome), so that’s why he wants to fight so hard his previous obsession for Constance and Loic. He really wants to begin a new life, but the trust that he receives is such an important condition that he either manages to overcome his past or not. And it turns into a life-death situation.

I still don’t know what to think about the neutral ending. Is it just meme material? Is it good instead? Do Ivan and Loic deserve each other? Probably they do, but there’s someone else suffering here. I somehow like it, but if I were an Ivan stan, I’d be probably hurt.

Aaron

Most people’s favourite, and the best beginning, but... I’m so frustrated! It’s easy to compare the first chapters with Vladimir’s, because the main lore doesn’t need to go as slow now that we already know it. Even so, I put too many hopes for him. I expected a tavern scene, but the “trying to impress you in Moondance” was also cool.

And we got interesting stuff coming. His story, the naturality of being bisexual and our Chalice’s attraction towards him, both’s relationship with Farah... Until we were forced to see her as a rival too. WHY?! Where is the feminism that we were promised? Love rivals are boring! And Farah herself said that she didn’t want to compete. I found her cool, but it’s as if we were playing A6 and its dumb self-inserts.

I still have some expectations about the ending but not too many, Rather than having a choice to express attraction towards Farah or not, we were forced. Perhaps my problem is that I wanted to voice my thoughts, and this is a pre-made Eloise.

Raphael

No, no, no... I’m really sorry for his fans because they are really cool people, but I didn’t want Leiftan 2.0, and I’m getting Leiftan after buying stuff from Nathaniel.

I can’t judge because it’s too soon, but I really need an explanation. I have various hypothesis:

a) As “religious” as he is, Raphael thinks that a Chalice is some kind of miraculous gift, so he’s in an exthasis state.

b) Considering that some people confirmed that there’s a past lover again, Raphael is having as much trouble as Ivan. He could even compare the two women if they have physical resemblance, but I don’t know.

c) With one of the previous conditions, the vampire-chalice bond has become so strong all of a sudden that she feels the strong emotions as well. She wakes up already infatuated because he’s been feeling that for a while, looking at her unconscious form.

d) Much simpler, it’s manipulation again, we know that he’s good at mental stuff with humans. That would mean that he’ll have to respect her soon, if this is properly adressed.

Either way, I’m happy that the past lover seems to be an unknown woman and not Alessio, because his design is somehow interesting, and I want to know his role in the story. But I’m still mad that Eloise hates everyone but Raph.

Fortunately, this time we can choose our reaction with Ivan instead of what happens in the other routes. We can find a middle ground where she isn’t excessively comprehensive nor harsh.

Ethan

Here comes my bastard! He doesn’t have a route yet, but we’re learning so much from him. And I’ll defend him, because I just like relatable bastards (like with a certain SnK character).

We know his main behavior is caused by his war trauma. It’ll depend on how the writer manages him. Hopefully he won’t treat his chalice very heartlessly, but she’ll be scared at first for sure.

He’s seen the worst side of humanity, had a tough time adapting, and he cannot rest. There are two recently converted people that are a reflex of humanity and himself, so he’s conflicted. We know he respects Eloise and becomes closer with her in Beliath’s route, and some of the other vampires appreciate him as well.

From his point of view, Ivan doesn’t even try to survive (it’s practically true in his bad ending, and almost false in the good one). Ivan has technically put all the manor in danger and killed someone, so once the biggest danger happens, he snaps. He doesn’t even know how to react, so the first thing that comes to his mind is scaring Eloise or breaking a few bones if necessary, so Ivan stops going into a trap, or at least, it doesn’t affect the other members. Really twisted choice, but it comes in a rush.

He’s suffering the “only love-interests” problem of this kind of otomes. Since there are almost no secondary characters, he has to be the one to do the harsh comments. I don’t want to mention routes 4-5, but in Vlad’s he seems to snap when Neil’s situation is too badly handled.

So, by now you must already know which writer I prefer, and perhaps it surprises you. But I’ll add something more. The infamous list of ML’s incoherent things.

First writer’s mistakes:

”A chalice can’t kill their vampire”. Vladimir’s neutral ending proves otherwise, unless we acknowledge that euthanasia is consensual assistance. I still don’t like how much they make it look too romantic, but I understand the point.

”A chalice is only free when the vampire dies”. Ivan’s neutral ending has Eloise being “replaced” by Loic, and that has no explanation at all.

Neil’s disappearance.

Second writer’s mistakes:

”Vampires can’t have children, unless they are hybrids”. What about Beliath’s father? He was 100% vampire, otherwise Bel would be a mixture of more races.

There is an unwritten law about chalices not getting romantically involved with other vampires. Perhaps it’s just a matter of respect, but Ethan explains it as if it were as bad as drinking their blood. Was Beliath’s comment just a joke, then? Quite weird, anyways.

”You will be given more options in the story to make it more visual-novel-like, so you can choose more freely”. I don’t know about the endings, but the illus are harder to obtain than ever. Failing Aaron’s second illu? Raphael’s first? Or is it just Beemov having bugs again?

Go up again and stare at the pic. Now look at my bastard’s scared face. I don’t want him to have another past lover, or to act too out of character, even if he has a problem.

[vgckwb]

ML: Isolation Chapter 8: Deals

Arien walked into class the next day to see a crowd had gathered around Ivan. He decided to follow suit. The reason why became apparent, as Ivan had some scars on his face. “Ivan, what happened?”

Ivan smiled and said “Buster did it.”

Adrien was confused. “Allow me to explain,” Mylene said. “See, Ivan asked to help volunteer with me yesterday. I was going to the animal shelter, and I thought I had a good idea. See, some of the bigger dogs get a little too energetic, so I thought Ivan could play with them for a bit to help calm them down. And he was doing a great job, but Buster, one of the biggest ones, got too into it and scratched him.”

“I see,” Adrien said.

“It was a complete accident, and Buster even felt sorry about the whole thing,” Ivan said.

“Yeah, Ivan’s been really forgiving about this whole thing” Mylene said.

“Why don’t you show them the pictures?” Ivan asked.

“Pictures?” Alix asked.

Ivan nodded. He looked at Mylene. “Oh, alright,” Mylene said. “I was worried because they were all from before the scar, but since you say it’s OK…” she took out her phone and everyone gathered around her. Mylene showed off a series of photos of Ivan playing with dogs. It was completely adorable.

“I gotta say, I didn’t think you had it in you,” Alix said.

Ivan blushed. “I wasn’t sure I had it in me either. But honestly, it was a lot of fun.”

“It’s all thanks to Adrien here,” Kim said.

Adrien blushed. “Who me? I didn’t do anything.”

“That’s not true,” Ivan said. “Your talk inspired me to help, and I want to thank you for it.”

“Oh, well, thanks,” Adrien said.

“You’re a natural,” Kim said.

“Yeah” said Alix. “I mean, Lila’s been great and all, but I’ve never seen a result this dramatic this fast. Maybe she could learn a thing or two from you.”

Lila was surprised. She blushed, rubbed the back of her head, and said “Maybe.”

“We’ll see,” Adrien said. “I mean, I imagine we’re both pretty busy. From what I’ve heard, after school, your life is an adventure!”

“I’m sure we can make the time,” Lila said. “I mean, you’ve gotten to know the class, and you’ve only been here for a week or so.”

“That’s true. A lot has happened” Adrien said. What would happen if Lila and I were alone? She can’t know I’m on to her, can she? If she doesn’t know, I don’t think she would cue me in on her goal just yet. It’s still too early. Still, I should be on guard for that meeting. “Well, if our schedules allow for some one on one time, I guess we could talk.”

Lila blushed. “I’d like that.” Shortly after class began for the day.

During break, Ivan met up with Adrien alone. “Hey, thanks again man” he said.

“No problem” Adrien replied.

“I mean it” Ivan said. “I know I only asked to try and get closer to Mylene, but the whole experience was rewarding.”

“I’m glad you liked it,” Adrien said. Ivan then grew concerned. “What’s up?”

“Well… If I tell you something, can you promise not to tell anyone else?” Ivan asked.

Adrien smiled. “Of course.”

Ivan smiled back. “Thanks. ...It’s just, you kind of remind me of Marinette. You know, before she...well…”

Adrien was surprised. “Huh.”

“It’s just, she used to be so helpful,” Ivan said. “I guess she was, even when she was jealous of Lila. Don’t get me wrong, she shouldn’t have bullied Lila, or stolen from her, but if you were to talk to her outside of Lila, it was like talking to you right now.”

“I see…” Adrien said, pondering this.

“I mean, Lila’s been helpful,” Ivan said, “it’s just...uh...how do I say this? She is a nice person, but she’s not as good at it?”

Adrien smiled. “It’s OK. Remember, this is staying between the two of us.”

Ivan smiled. “Thanks. Well, we should get going.”

“Alright,” Adrien said. Ivan left. Adrien looked around and saw Lila standing by herself. He decided to walk over and say “Hello.” Lila looked at him curiously. “Looks like we’ll get that chance to talk sooner than we thought.”

“I guess” Lila smiled. She looked sad. “So, how do you do it?”

“Do what?” Adrien asked.

“Well, you heard the class today,” Lila said. “How do you help people so well?”

Adrien had to tread lightly. I don’t want her to undermine me on any future attempts to deconstruct her web. I still don’t even know how big it is yet. It would be foolish to make it bigger before my eyes. I just have to give a vague answer. “Well, I just listen and give positive reinforcement,” he said.

“I try to do that too,” Lila said. “But you’ve helped Ivan in a way I couldn’t. Why is that?”

She’s trying to get a more specific answer. Just keep it vague, and we should be fine for now. “Well, I guess that’s just because we’re different people,” he said. “We have different experiences, and as such, we would have different ways of helping people.”

“Huh” said Lila. “That wasn’t what I was expecting, but I guess that makes sense.”

Adrien smiled. “Tell you what. If you’re helping someone out, why don’t you text me or something? That way, we can work together! You showing off your strengths, and I showing off mine. I mean, two heads are better than one after all.”

Lilai was taken aback. She blushed and said “I’d like that.”

“I’d like that too,” Adrien said.

“Well, we should get back to class,” Lila said.

“OK” Adrien said. He started walking when he noticed Lila grabbing his arm. “Um, Lila?”

“Huh? Oh” Lila said. She let go. “Sorry. I just get nervous easily. You know, ever since the incident. And you just seem so brave. So, I just kind of…”

Adrien smiled. “I understand. Just make sure to ask. I’m not great with people touching me unexpectedly. Between my fans and Chloe, it started feeling uncomfortable.”

“Oh no,” Lila said. “Don’t worry. I’ll have a nice chat with Chloe about this.”

“You don’t have to,” Adrien said. Lila was surprised. “What I mean is, I’ve already talked to her about it, and she’s gotten better about it.” Lila smiled and nodded and the two walked off.

OK, I don’t think she’s on to me, and I’ve laid a trap. Sure, it’s double edged, and whether or not she’ll spring it is up in the air, but it’s still worth trying. Still, she knows one of my weaknesses now. It’s not a weakness she can use well, but I should be careful regardless.

After school that day, he hung out with Nino at the dance club. They taught each other more about the dancing styles they knew, and had another good time all around. However, when it came time for Adrien to leave, Nino rushed him out again. Once could be coincidence, but twice now? Something’s up. I just need to find a good time to ask about it.

He then got out his phone. He was going to message his bodyguard but decided to do a quick check of Noir Notes to see if there was anything new. He had now got in the habit of bringing his costume along, so if there was something he could do, he could do it.

Nothing around him looked like he was needed, and anything new on the site was more speculation, which was wildly varying, and more requests. Again, most of these requests were trivial in nature. But there was a new one that caught his eye due to how odd it was.

“Hello? Cat Noir? Um, I have a note I want to deliver to someone, but I don’t know how. Could you possibly do it for me? I’ll be in the TV station lobby tonight until 6:30. Thank you.”

Adrien was puzzled. He checked the time stamp. Today. And it’s about 5:45 now… He realized the Kids+ TV studio wasn’t too far from here. Well, let’s just hope this is the right one… He messaged his bodyguard saying to pick him up there at about 6:25, and went on his way.

Once he got there, he hid on the side of the building and dressed up, leaving his bag behind. As Cat Noir, he walked into the building. He approached the receptionist and rang the bell. The receptionist looked up confused and surprised.

“Pssst! Over here” said a voice. He looked over to see a young girl waving at him. Adrien was now the one who was confused and surprised. He walked over. He saw she had a lot of paper and some crayons for drawing. “I knew you’d make it!”

Adrien smiled. I love her optimism Although, she is a child, so she probably doesn’t know it was dumb luck that brought me here. I won’t tell her though. He grabbed some of the paper and a black crayon and decided to write his statements, stylizing them like a child. “How did you get onto Noir Notes?”

“Um, I saw mommy doing some research on there. She’s a news reporter! She gave me this” she said, holding up a tablet, “to watch some videos on, but I really needed to get this note out” she now held up her note. “So, I logged on and tried my best.”

Adrien smiled for her. “You did good kid. What’s your name?”

“Manon” she said. “Anyway, can you deliver this?” She held up the letter.

“Who’s it for?”

Manon looked sad. “It’s for my old babysitter,” she said. “I don’t know if you know her, but her parents run the bakery you went to.” Adrien was surprised. “Mommy said she did something bad, and that she’s not allowed to babysit me anymore. I know she didn’t do it though! She’s so nice! I wanted to deliver it to her, but I don’t know how to get there without getting a ride, and mommy won’t help me mail it. So, please?” She held out the note again, this time with her baby-doll eyes.

How could I say no to that face? He wrote “Do you need to get it to her ASAP?”

“A...SAP?”

Adrien silently chuckled. “As Soon As Possible. It means does she need it right away?”

“Oh. Well, I would like for her to get it right away, but as long as she gets it, I’ll be alright.”

“OK. Thank you. I will pass it along to her.”

Manon’s face lit up. She was so giddy she was just about to charge in for a hug. She then stopped herself and asked “Um, can I give you a hug?” Adrien nodded. Manon rushed in and hugged with vigor. Adrien hugged back.

Adrien hadn’t even considered something like this. I promise you, the two of you will be reunited. It may take some time, but I will do it. He let go and took the note. He did a two finger salute to say his goodbye and left.

He went to the side of the building to grab his bag. However, he bumped into someone. He looked up to see that it was his bodyguard, holding his bag. The two looked at each other for a little bit. Adrien surmised that he came by and saw the bag on the ground and went to investigate. Despite his appearances, Adrien knew his bodyguard wasn’t stupid. There wasn’t really a lot he could do in this situation. He sighed. “Can you at least not tell my parents and only talk to Nathalie about this?” The bodyguard was confused, but nodded. Adrien changed out of his disguise, and put it back in the bag. The two went to the car and headed home.

On his way back, Adrien messaged Marinette. “Hey, we have a bit of a situation. I got caught as Cat Noir by my bodyguard. I managed to convince him to only tell my family’s assistant, but I can’t lie to them. Could I just tell them about you? If that’s alright? Please get back to me ASAP.”

Later, Adrien was meeting with Nathalie. Adrien’s costume was on the table, along with his phone. Nathalie was rubbing her eyes. “Adrien. Please tell me you have an explanation for all of this.”

“I do,” Adrien said.

Nathalie put her glasses on and glared at him. “And?”

“Oh. Oh you want me to TELL YOU my explanation” he said. “Well...um, this is gonna be difficult.” Marinette hasn’t gotten back to me yet. I don’t know if I can keep this up.

“I can’t in good conscience hide this from your parents,” Nathalie said. “Please explain to me what possessed you to do any of this.”

“Ummmmmmmm...”

Suddenly the phone rang. Adrien reached for it, but Nathalie grabbed it first, as if her hand was as fast as hurricane winds. She noticed it wasn’t a registered number. She answered “How did you get this number?”

“Um, Adrien gave it to me” Marinette said timidly from the other line. “You’re the secretary right?” Nathalie was alarmed. “You’re talking about him being Cat Noir… Well, that’s kind of my fault.”

Adrien was worried about what was happening on the other end. He tried reaching for the phone, but Nathalie blocked him. She continued to listen to Marinette. “I see...Thank you. Have a good day.” She hung up. She sighed. “Adrien. I know you can be a bit of a problem child.” Adrien remembered several times in which he attempted to sneak out or run away just to be on his own for a little bit, causing his parents, his bodyguard, and Nathalie to worry. “But THIS just takes the cake.” Adrien looked worried. “I’ve heard what your friend Marinette had to say. Now let’s hear you explain it.”

Adrien was puzzled. “Well, so, you know how Ladybug is my favorite designer for father’s company?” Nathalie nodded. “Well, I’ve been wanting to meet with her for a while now. I figured out her name, but not much else. Then, when I went to school, I caught her catching a glimpse of me. I learned that Marinette was considered a bully, and then later, I was told by father that Ladybug had new designs for me. I knew then they were the same person.”

Adrien explained the rest of the story. How he couldn’t be seen going to the bakery, and how he felt so desperate to meet with her. The origin of the disguise. The whole Lila thing. He hoped that it got through to her.

Once he was finished, Nathalie composed herself. “Well, at least the two of you are good at corroborating, if nothing else.”

“But it’s the truth!” Adrien said.

Nathalie smiled. “I know. I can tell when you’re lying.” She sighed. “Alright, I won’t tell your parents about all of this, at least for now. But I want you to promise me something.”

Adrien was confused. “What?”

“I want you to talk to your parents more about what’s going on,” she said. “You don’t have to explain everything, at least not yet. Don’t get me wrong, I want you to, but I understand why you won’t for now.”

Adrien looked sad. “Adrien,” she said. He looked up. “They’re your parents. They care about you. They want to hear about how you’re doing. Perhaps they can even help you. Of course, I’ll be willing to help you too.” Adrien looked excited. “If what you said about this Lila person is true, then I wish to help you. I know a thing or two about behind the scenes work. Your bodyguard will also be able to help. Isn’t that right?” She looked at the bodyguard, and he nodded. “Just be sure you’re keeping up with school, and all of your other stuff.” Adrien nodded. “You’re going to be really busy. Are you sure you can handle it?”

Adrien grinned. “When you’ve had nothing to do for most of your life, there’s a lot of pent up energy to do all of this.”

Nathalie smiled a silly grin. Adrien was perplexed, because that smile was rare. “You know, I sat in on some of the meetings where your parents were discussing you going to school. Your father was worried about all sorts of things. I thought he had run through every possible scenario. I guess I was wrong.”

Adrien smiled. “Oh yeah” he said. He took Manon’s note out of his pocket. “Um, I’m supposed to deliver this to Marinette. Is that alright?”

Nathale smiled. “Don't worry Adrien, I’ll take care of this. Your father wanted me to investigate the bakery anyway. You know, after your little stunt.” Adrien blushed. Nathalie grabbed the note. “Alright. Just do whatever you were going to do when you got back before all of this. And remember what I said.”

Adrien nodded. “Thank you,” he said.

Nathalie smiled. “Let’s go!” she told the bodyguard. The two of them headed out.

Nathalie and the bodyguard had managed to get to the bakery before it closed. Nathalie walked in alone. Sabine and Tom turned to her. “Um, hello. How can we help you?”

“I know this is a bit sudden,” Nathlie said, “but I work with Gabriel Agreste. Because of the recent attention brought upon you, he wants to know about his place. I know that you’re closing now, but is there a good time to set up a meeting?”

Tom and Sabine were shocked. “Um, what would this meeting be about?” Tom stammered out.

“Mr. Agreste likes to consider a lot of possibilities” Nathalie said. “He was wondering about using your bakery as a set. Or barring that, using some of your goods for a baking motif.” Tom and Sabine looked at each other, not sure about what to do. “I will tell you, a lot of this goes nowhere. But, if you’re interested in the possibility, here’s my card,” she said, handing it to them. “Also, I found this outside your door. It’s addressed to a Marinette.”

“That’s our daughter,” Sabine said. “I’ll make sure it gets to her.”

Nathalie smiled. “Thank you. Also, if it wouldn’t be any trouble, could I get a box of cookies.”

“Oh, it’ll be no trouble at all,” Tom said. He left to get them.

“You know, Mr. Agreste’s son came in and he also got cookies,” Sabine said.

“So I’ve heard,” Nathalie said. “He told me they were delicious. I’m curious.”

“Here you go,” Tom said.

Nathalie paid for them. “Thank you.” She left the store and got in the car. They pulled off. Nathalie started eating the cookies. “Want one?” she said, offering one to the bodyguard. He rejected. She continued eating them.

The bodyguard then gave her a stern look. “Don’t look at me like that,” she said. “Look, I know it’s crazy, but I really want to help Adrien here. Was he foolish? Of course. But being young is all about making mistakes. I’m just here to make sure he’s making the right ones.” She smiled. “Besides, can’t you tell?” The bodyguard raised his eyebrow. “Our little Adrien’s in love.” The bodyguard thought about it, and then nodded. “I’m just not sure he knows it yet.”

Adrien went back to his room as soon as the meeting was over. He headed to the computer and saw Marinette messaged him. “Adrien! Are you alright? What happened?”

“I’m still trying to process that myself,” he replied. “But basically, not only has Nathalie agreed to not tell my parents, she’s willing to help me.”

“...? Why?”

“I’m not sure. But I’m glad she is.”

“Well, I’m glad things worked out for you.”

“Sorry about dragging more people into this…”

“It’s OK. This was sudden. I get it.”

“By the way, the mission I was on as Cat Noir involved you.”

“Me?”

“Yup. I was to deliver a note to you. But Nathalie’s taking it to your place.She said she needed to scout it anyway. You should be getting it soon.”

“Thanks.”

“You’re welcome. Good night.”

“Good night Adrien” she typed. She saw Adrien log off. She then spoke to herself. “What could that be?”

“Marinette!” her mom called. “There’s a letter for you! I’ll just slip it through the door as usual.” Marinette rushed over and opened the hatch door. Sabine was surprised to see her. “Marinette?”

“Uh, yeah. I just wanted to get this from you.” She took the note. “Thank you, mama!” She kissed her mom on the head, and closed the door.

Sabine was surprised. “Well, it’s good to see she's getting better.”

Marinette went up to her bed and looked at the note. “Manon?” she said, recognizing the handwriting. She opened it up.

“Marinette, Mommy says you did something bad, and you can’t be my babysitter anymore. I don’t think that’s true though. You’re so nice and kind. I can’t believe that you would do something so mean.

Mommy says you haven’t left your house much since got kicked out of school. You taught me that sometimes people just need to be alone sometimes. But I do want to see you again. I want to show you how much I’ve grown since the last time I’ve seen you. Please come out soon. Once they realize you didn’t do it, I’m sure they’ll forgive you. -Manon”

Underneath was a drawing of the two of them. Marinette was impressed, and at the same time heartbroken. Manon still believed in her. She couldn’t believe it herself. Manon looked up to her so much, and wanted her to leave. Meanwhile, here she was wallowing in self-pity. Marinette began crying. “I’m sorry Manon.”

[kathasofwander]

A ML MCU Crossover

It all started with a snap.

Chapter 2

The next three weeks were a blur. Manon ran into the bakery crying that Mommy vanished while feeding her lunch. Jagged Stone dropped in, panicking because Fang and Penny had 'magically dusted away'. More and more people who the four knew started coming to the bakery. Luka, Chloe, Kagami, Ivan, Kim, Marc, etc.

Adrien was devastated because all three of his guardians had been dusted away, and he was all alone. Nino and Alya took the news of Marinette and Adrien being superheroes very well. They were more then glad that Paris's beloved heroes were someone they trusted so much.

A quick trip Master Fu's house revealed that the old guardian had vanished too. Marinette took the miraculous box with her for safety. Master Fu's miraculous lay on the ground, and Marinette understood what that meant.

"We have to do something." Adrien said, as he, Marinette, Alya, Nino, Luka, Kagami and Chloe sat on the couch. (Manon was sleeping in Marinette's room while the others had been accomodated in the other rooms in the building.)

"I know." Mari said, "But what? Hawkmoth hasn't been active either. We don't know for sure what has happened." She finished. Just then, there was a cackling noise. A golden portal opened in the kitchen and alerted the seven. Mari and Adrien transformed. "Get back!" They shouted at the others. A few people stepped out of the portal. A large well built man. A Chinese man, a woman with blonde hair, another woman with silver hair, another man who had an axe, and...a raccoon??

"The Avengers?" Alya asked. The well built man nodded. "Miss Dupain Cheng? Mr. Agreste?" He asked. "We come in peace" he said. The two heroes dropped their transformations. There was really no point when these people knew their identities. However, how did they? "How??" Mari whispered. Thank god for Jagged Stone and his English lessons. "Tony Stark is a genius." The raccoon answered. "He pulled out your footages and even figured out who you both really are." One of the women answered.

"We need you to come with us." The other woman continued. "How can we trust you?" Adrien asked them. "We know that you are scared. But there is an explanation for all this. And your cataclysm and your luck could prove useful."

Marinette nodded. If this meant then everyone will come back, then she was willing to do anything, and she knew that so did Adrien. "All right. But first there's something we need to do. She turned to the other five and beckoned them to the other side of the room.

Marinette opened the miraculous box and handed the other miraculous out. "Keep them. In case Hawkmoth decides to create another Akuma, I need you to defeat it, and keep the akuma for me to purify when I come back. Alya, Nino. You both will lead the team. We trust you." The five nodded at them, and Marinette and Adrien entered the golden portal, hoping to find a way to rewind this disaster.

A/N: This was a long ass chapter. But hopefully it's good. The next chapter will be a bit smaller, and then the 5 year leap it was in Avengers Endgame. In the meantime, enjoyyyy!

[incorrectml]

bel’s nicknames for ivan: 11/10

[ao3feed-lukanette]

by xxoceanswavesxx

Summer is finally over. With school back in full swing, Marinette finds herself more at a loss with new allies, enemies, and wild cards with their own agendas.

Sequel to "All the Laughs We Had in the Past".

Words: 4331, Chapters: 1/5, Language: English

Series: Part 7 of The One to Make It Stay

Fandoms: Miraculous Ladybug

Rating: Not Rated

Warnings: Creator Chose Not To Use Archive Warnings

Categories: F/M

Characters: Marinette Dupain-Cheng | Ladybug, Luka Couffaine, Rose Lavilliant, Juleka Couffaine, Ivan Bruel, Jagged Stone (Miraculous Ladybug), Penny Rolling, Original Characters, Aurore Beauréal, Mireille Caquet, Lila Rossi, Kagami Tsurugi, Max Kanté, Vivica (Miraculous Ladybug)

Relationships: Luka Couffaine/Marinette Dupain-Cheng | Ladybug

Additional Tags: Lukanette, ml salt, ml salt fic, ml s3 au, Kitty Section

[animegenork]

Do you ever want to give someone a hug so badly that you’d do anything? Just so they can smile and stop berating themselves so much?

Because that’s how I feel about my sad baby boi Ivan.

If I have anything to say about it, I’m going to make him smile.

[binnedrubbish]

5/12/19 Notes

Lab Meeting Prep Pipeline:

(May 2nd, 2019 at 2:38 p.m.) 

[ ] Read the Results & Discussion cover to cover

[ ] Complete slides for all figures

[ ] Give a practice presentation

[ ] Read methods 

[ ] Complete fluorescence slides

[ ] Decide how to deal with ‘relationship between calcium activity and movement’ section

[ ] Give a practice presentation 

[  ] Read supplementary material cover to cover

[  ] Give a practice presentation 

Note to self: Relax.  Be meticulous.   Be disciplined.  Keep calm, do your best, trust your team.  

—— 

——

Advanced Optimization 

8 20 905

Live Action Poem, February 2nd, 6:41

Went to Brazil out of spite and saw

stone Jesus, arms open for a hug,

bought street weed, twice, from the same vendor

out of a reckless love for reckless love.

Hoped for a tropical muse and found 

a strong handshake from a dangerous man.

Holed up in Rio de Janeiro with piles

of paper money and paced all alone

angry at nothing if only for the moment.

Rain dampened slick stone walkaways,

waiters were too nice and I tipped too much.

One offered to be a bodyguard , violence

hinted in every smirking human moment.

God, I loved being a target, smug,

dumb, flitting away American Dollars.

Jesus Christ looming in stone on a hill top.

Titties and marijuana, iconic primadonna 

extravagant flora, dying fauna, fawning

over the climate. I went to Brazil

on an off month. To hole up 

safe from my sprawling little lovely life. 

To Do 26.1.19

[x] Cristina - Search for Hippocampus Models

[x] Ana G. - Draft e-mail call for interest in “Live Action Science”

[  ] 

Data science Club Thursday at 5:00 p.m. 

Astavakrasana 

laser-scanning photostimulation (LSPS) by UV glutamate uncaging. 

12.1.19 Goals

[x] Some Portuguese 

[x] Mouse Academy - first read 

[ / ] Dynamic mesolithic dopamine 

[x] Water rats * SMH

Acorn - tracks impact | BetaWorks | 2 years of money | PitchBook | Social Impact Start Up 

Mission Aligned Investors | Metrics | Costumer Acquistion Cost | Clint Corver -> Chain of Contacts -> Who To Talk to (Scope: ~100) 

Money Committed || Sparrow || Decision Analysis —> Ulu Ventures [500k] [Budget x ] 

Ivan - > IoS Engineering { Bulgarian DevShop } 

[market mapping] Metrics -> Shrug 

Peter Singer - Academic Advisory Board … 

[1 million ]

Product market testing 

Foundation Directory Online  - Targeted , Do Your Homework 

https://www.simonsfoundation.org/2018/11/19/why-neuroscience-needs-data-scientists/

Head-fixed —> 

~INHIBITION EXPERIMENT TRAINING PLAN~

STOP MICE:  20th.  GIVE WATER: 20th (afternoon) - 30th.  DEPRIVE: 31st... (Morning) RESUME: Jan 2nd.

21st - BLEACH/DEEP CLEAN BOXES 1-14 (Diluted bleach; Flush (with needles out) - Open Arduino Sketch with Continuously open Valves - PERFUSE System) *[NOT BOX 11 or 5]*; Run 15 mL of Bleach per syringe; Copious water through valves; Leave dry.

———

http://www.jneurosci.org/content/preparing-manuscript#journalclub

Friday - Dec. 14th, 2018 

[x] - Complete 2019 ‘Goals and Blueprint’ 

[x] - 2-minute Summary ‘Properties of Neuron in External Globus Pallidus Can Support Optimal Action Selection 

[  ] MatLab for Neuroscientists :: Basic Bayesian Bearded Terrorist probability plots 

[x] Statistics 101: Linear Regression 

“Golden Girls” - Devendra Banhart

“King” by Moor - FIREBEAT 

Reread - Section 3.3 to  

Monday - Apply for DGAV License (MAKE SHORT CV)

SAMPLE: ‘Sal’ From Khan Academy 

Make short CV

Tiago - Certificate 

MATH:

“We explicitly focus on a gentle introduction here, as it serves our purposes. If you are in 

need of a more rigorous or comprehensive treatment, we refer you to Mathematics for Neuroscientists by Gabbiani and Cox. If you want to see what math education could be like, centered on great explanations that build intuition, we recommend Math, Better Explained by Kalid Azad.”

Jacksonian March seizure (somatosensory) 

—

Tara LeGates > D1/D2 Synapses

Scott Thompson

Fabrizio Gabbiani - Biophysics - Sophisticated and reasonable approach 

Quote For Neuroscience Paper:

“Every moment happens twice: inside and outside, and they are two different histories.”

— Zadie Smith, White Teeth  

Model Animal: Dragonfly? Cats. Alligators. 

Ali Farke Toure 

Entre as 9 hora e o meio-dia ele trabalha no computador. 

Ele volta para  o trabalha à uma e meia.  

Ele vai as compras depois do trabalho.

A noite, depois do jantar, ele e a mulher veem televisão.

As oito vou de bicicleta para o trabalho.  (go)

As oito venho de bicicleta para o trabalho.  (come) 

A que horas começa a trabalhar?

Eu começo a trabalhar os oito e meia.

Normalmente… 

Eu caminho cerca de Lisbon.

É muito triste! Eu faço nada! Talvez, eu caminho cerca de Lisbon.  Talvez eu leio um livro.  Talvez eu dormi.    Eu vai Lx Factory.  

Depois de/do (after) 

antes de/do (before) 

—

Monday -> Mice 

MATLAB!

-

“New ways of thinking about familiar problems.” 

~*NOVEMBER GOALS*~ 

> Permanent MatLab Access [x] -> Tiago has license 

> Order Mouse Lines [ ] -> Health report requested… Reach out to Vivarium about FoxP2 

   -> Mash1 line -> FoxP2 expression?  

> Finish ‘First Read Through’ [ ] 

> Figure 40 [ ]

SAMPLE : ‘Afraid of Us’ Jonwayne, Zeroh 

Monday Nov 5th Goals: 

> Attentively watch:

> https://www.youtube.com/watch?v=ba_l8IKoMvU (Distributed RL)

> https://www.youtube.com/watch?v=bsuvM1jO-4w (Distributed RL | The Algorithm) 

MatLab License 

Practical Sessions at the CCU for the Unknown between 19 - 22 Nov 2018 (provisional programme attached)

Week of November 5th - Handle Bruno’s Animals 

Lab Goals - 

“Deep Networks - Influence Politics Around the World”

Paton Lab Meeting Archives

Strategy: Read titles/abstracts follow gut on interesting and relevant papers

Goals: Get a general sense of the intellectual history of the lab, thought/project trajectories, researchers and work done in the field and neighboring fields.

Look through a GPe/Arkypallidal lens… what can be revisited with new understanding?

First Read Through 

[x] 2011 - (22 meetings || 10/12 - SLAM camera tracking techniques)  

[ x] 2012a (18 meetings) 

 [x] 2012b (15 meetings - sloppy summary sentences)

[ x] 2013a (19 meetings - less sloppy summaries jotted down)

[x] 2013b (17 meetings) 

[x] 2014a (21 meetings) (summaries in progress)

[x] 2014b 

[x] 2015 (23 meetings)

[ ] 2016 (23 meetings) 

Current 

—

“I like, I wish, I wonder”

“Only Yesterday” Pretty Lights

retrosplenial dysgranular cx (?)

retrosplenial granular cx, c (?)

fornix (?)

Stringer 2018 arVix

Lowe and Glimpsher 

November Goals:

[  ] GPe literature - 

[ x ] Dodson & Magill

[  x] Mastro & Gittis

[  ] Chu & Bevan 

[x] Modeling (extra credit -Bogacz)

[  ] Principles of Neural Science: Part IV

[ x ] MatLab license… Website program… 

Extra credit:

Side projects [/ ] Neuroanatomy 40

[ -> ] ExperiMentor - Riberio, Mainen scripts… Paton! -> LiveAction Science

MACHINE LEARNING 

Week of Oct 29th - 

Symposium Week!

Wyatt -> John Hopkins -> He got into American University! 

Belly Full Beat (MadLib album Drive In) 

“The human brain produces in 30 seconds as much data as the Hubble Space Telescope has produced in its lifetime.” 

Sequence of voltage sensors -> ArcLite -> Quasar -> Asap -> Voltron -> ???

Muscarine -> Glutamate 

Ph Sensitive 

cAMP

Zinc sensitive 

5 ways to calculate delta f

2 main ways 

SNR Voltage — 

Dimensionality reduction of a data set: When is it spiking?

5 to 10 2-photon microscope open crystal 

…Open window to a million neuron…

Week of 10/15/18

Monday: Travel

Tuesday: Rest

Wednesday: Begin rat training.  Reorient.

Thursday:

Friday:

|| Software synergistically ||

—————

Beam splitter, Lambda, diacritic 

1.6021766208×10−19

‘sparse coding’

Benny Boy get your programming shit together. 

Week of Oct. 8th, 2018

10/9/18

[  ] Rat shadowing (9:30 a.m.) -> Pushed to next week 

10/8/18

[x] Begin Chapter 13 of Kandel, Schwartz, Jessell

[x] Outline of figure 36

[  ] Read Abdi & Mallet (2015) 

DOPE BEAT MATERIAL - Etude 1 (Nico Muhly, Nadia Sirota) 

Saturday - Chill [x]

Friday - ExperiMentor … mehhhhh scripts?  

Photometry -> Photodiode collects light in form of voltage (GCaMP) (TtdTomate as Baseline… how much fluorescence is based on TdTomatoe, controlling factor always luminesce - GCaMP calcium dependent) :: Collecting from a ‘cone’ or geometric region in the brain.  Data stored and plotted over time… Signals must be corrected… 

Cell populations are firing or releasing calcium.  (GCaMP encoded by virus injection, mice express CRE in a particular cell type).  

———————————————

———————————————

Brain on an Occam’s Razor,

bird on a wire, 

synaptic fatalism integrating 

consistent spiking;

strange looping: is this me? 

Thursday 

“We don’t make decisions, so much as our decisions make us.”

“Blind flies don’t like to fly”

[x] 9:00 a.m. Lab Meeting

[x] 12:00 p.m. - Colloquium

“It was demeaning, to borrow a line from the poet A. R. Ammons, to allow one’s Weltanschauung to be noticeably wobbled.”

“You must not fear, hold back, count or be a miser with your thoughts and feelings. It is also true that creation comes from an overflow, so you have to learn to intake, to imbibe, to nourish yourself and not be afraid of fullness. The fullness is like a tidal wave which then carries you, sweeps you into experience and into writing. Permit yourself to flow and overflow, allow for the rise in temperature, all the expansions and intensifications. Something is always born of excess: great art was born of great terrors, great loneliness, great inhibitions, instabilities, and it always balances them. If it seems to you that I move in a world of certitudes, you, par contre, must benefit from the great privilege of youth, which is that you move in a world of mysteries. But both must be ruled by faith.”

Anaïs Nin

[  ] MatLab trial expires in 1 day * 

[  ] 3:00 p.m. pictures

“We do not yet know whether Arkys relay Stop decisions from elsewhere, or are actively involved in forming those decisions. This is in part because the input pathways to Arkys remain to be determined.”

These studies prompt an interesting reflection about the benefits and conflicts of labeling and classifying neurons at a relatively grainy level of understanding.  

“The authors hypothesize that under normal conditions, hLTP serves an adaptive, homeostatic role to maintain a healthy balance between the hyperdirect and indirect pathway in the STN. However, after dopamine depletion, pathologically elevated cortical input to the STN triggers excessive induction of hLTP at GPe synapses, which becomes maladaptive to circuit function and contributes to or even exacerbates pathological oscillations.”

To Do Week of Oct. 1st - Focus: Big Picture Goals

[ x ] GPe Literature - Hernandez 2015 & Mallet 2016 (Focus on techniques and details)

[  ] MatLab! Lectures 6-7 (Get your hands dirty!)

[ x ] Kandel Chapters 12 - 13 

Tuesday Surgery Induction 10:00 with Andreia 

6:00 - 7:30 

Portuguese

Digitally reconstructed Neurons: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5106405/

To Do Week of, September 24th, 2018 

To Do Week of  Monday, September 17th, 2018

PRIORITY: 

DATA ANALYSIS PROJECT ITI 

———— PAUSE. ———————

Talks 

[x ] Mainen Lab - Evidence or Value based encoding of World State/Probability - ‘Consecutive failures’ - easy/medium/hard estimate of where the reward will be.  

Reading for the Week

[x] Chapter 9 - Propagating Signal | The Action Potential

[/ ] Ligaya et. al (2018)  (CCU S.I.?)

[x] Katz & Castillo (1952) Experiment where they describe measurement techniques

[  ] Raiser Chapter 4 - Stimulus Outlasting Calcium Dynamics in Drosophila Kenyon Cells Encode Odor Identity 

Video Lectures

[—  ] Linear Algebra (Trudge steadily through) 

[ — ] Khan Academy Logarithms (Trudge steadily through) 

MatLab

[  ] Trudge steadily through www.mathworks.com/help/matlab/learn_matlab 

*FIND PROBLEM SET/TEXT BOOK/WORK SHEETS*

Concepts to Grasp

[ / ] Master logarithms!

[  ] Review Kandel Et. Al  Part II *Chapters 5-9*

Neuroanatomy

[ x ]  Ink Figure 28

Project Planning?  Too soon! Too soon! Read some literature on the subject.  

17/9/18

1:00 p.m. Meet with Catarina to discuss “CCU Science Illustrated” (WIP) Project

2:30 p.m. Vivarium Induction 

_______________________________________________________

|      SPCAL Credentials     |

|   |

| login: |

| PW:   |

-————————————————————————

——

NPR:: https://www.npr.org/sections/health-shots/2018/09/11/644992109/can-a-barn-owl-s-brain-explain-why-kids-with-adhd-can-t-stay-focused

9.13.18

[ x ] Pauses in cholinergic interneuron firing exert an inhibitory control on stratal output in vivo (Zucca et. al  2018)

[ x ] Chapter 8 - Local Signaling: Passive Properties of 

-> Sub and supra threshold membrane potential (Conceptual) 

Monday, Sept. 10th 2018

“Eat the Frog First”

[ N/A ] Review SPCAL Lessons 1-5 (In Library?) CRAM THURSDAY? 

-> [/] wait for confirmation from Delores for theoretical test 

-> (Out of Office reply from person in charge)

To Do:

[/] Comment Out %PRE_PROCESS_vBeta.m 

[x] Change path name and run program in MatLab

[  ] Solve trial.blahblahblah error spkCount?  labels?

[  ] Change Epochs and run? 

[x] Chapter 7 - Membrane Potential :: Return to Pg. 136-137 Box 7-2 when sharp. ::

[x] Castillo and B. Katz (1954) 

[x] 12:00 - Neural Circuits for Vision in Action CCU

[x] 2:30 - THESIS DEFENSE: Mechanisms of Visual Perceptions in the Mouse Visual Cortex 

————

Extra-credit

[x] Ink Figure 24

[~ ] Finish “First & Last 2017” (100/127 = 78.74%)

——

Jax Laboratory Tools: https://www.jax.org/jax-mice-and-services/model-generation-services/crispr-cas9

Recommendation for Design and Analysis of In Vivo Electrophysiology Studies 

http://www.jneurosci.org/content/38/26/5837

On the Horizon: 

Schultz (1997) (Classic, classic, classic) 

*[x] 9/7/18 - 6:00 p.m. Flip water for Bruno’s mice *

ITI Data Analysis -> Next step ->…. 

[  ] (find the sigmoid call) /  Poke around preprocessing_beta 

Reading 

[x] Chapter 6 - Ion Channels

[ / ] Finish Krietzer 2016 —> [  ] write an experiment-by-experiment summary paper

Resource: https://www.youtube.com/watch?v=GPsCVKhNvlA Helpful explanation of ChR2-YFP, NpHR, and general ontogenetic principles.

[ / ] Reiser Chapter 3.3.38 - 3.4 (Need to finish 3.4.5, Look up Photoionization detectors, Coherence) 

Neuroanatomy

[/]  Finish Figure 24 (need to ink)

“Drawing Scientists “

[/] Storyboard for GCAMP6s targeted paper 

-> Show Filipe for feedback ->

-> Ask Leopold permission ? Talk to Catarina 

[  x] 16:9 

[x] Write script and record [ 1:00 ] 

Intellectual Roaming

[ / ] Return to Review of Reviews and Review Zoom-In | First & Last | 

[/] Explore Digital Mouse Brain Atlas 

9/6/18 - Thursday 

To Do: 

ITI Data Analysis :

[x] Draw data structure on mm paper -> Reach out for help understanding 

[ / ] What fields did Asma call?  What fields are necessary for a psychometric curve

Reading 

[x] Kandel - Chapter 5 | Synthesis and Trafficking of Neuronal Proteins 

[ / ] Reiser - Chapter 3 | A High-Bandwidth Dual-Channel Olfactory Stimulator for Studying Temporal Sensitivity of Olfactory Processing (Results complicated) 

[/ ] Krietzer 2016 - Cell-Type-Specific Controls of Brainstem Locomotor Circuits by Basal Ganglia

Talks:

[x] 12:00 p.m.  - Colloquium - Development of Drosophila Motor Circuit 

Tutorials: 

~ [x ] MatLab plotting psychometric curves 

Neuroanatomy 

[ x ] Outline brain for figure 24

———

MatLab

Laser stuff HZ noise, thresholds, 

// PCA -> Co-variance -> 

// Linear regression | Geometric intuition -> “What is known to the animal during inter-trial?  What features can be described by animals history”  ===> Construct a history space (axis represent different animals history ex. x-axis previous stimulus, reward, etc.?)  Predictive (?)  

Plot psychometric functions || PSTH (post stimulation of histogram )  of example neurons -> skills: bin spiking, plot rasters, smoothing (if necessary) 

Data:: Access to Dropbox -> /data/TAFC/Combined02/ [3 animals :: Elife] 

/data/TAFC/video

Tiago and Flipe know the video data

File Format -> Parser/Transformation (guideline) || 

> MatLab

Access to MatLab -> [/] 28 days!

How can I begin to analysis?

History dependent | Omitted 

——

To Do Week of September 3rd

Monday

Administrative

[ x] Check-in with HR (Don’t bombard!): Badge.   (Library access?) 

[  ] Reach out to SEF?

[x] 2:00 p.m. Meet with Asma - discuss data analysis.  Where is it?  How do I access it (Tiago?)  What has been done and why?

[x] 3:00 p.m. Lab Meeting “Maurico’s Data” - Pay special attention 

[x] Finish first read through of Theoretical Laboratory Animal Science PDF Lectures

[  ] Rat Surgery Techniques…

Mouse neuroanatomy project

[/ ] Figure 24

[  ] Figure 28

Math 

[x ] L.A. Lecture 2

[ x] L.A. Lecture 3 

Read:

[  ] Georg Raiser’s Thesis (Page 22 of 213)

Find time to do at least an hour of quiet focused reading a day.  (Place?).

Continue to explore whims, papers, databases, ideas, protocols, that seem interesting. 

Develop ‘literature scour’ protocol - (Nature Neuroscience, Neuron, Journal of Neuroscience) 

Dates to Remember: September 14th - Laboratory Animal Sciences Theoretical Test! 

https://www.sciencedaily.com/releases/2018/08/180827180803.htm:Can these be used for techniques?  

https://www.sciencedaily.com/releases/2018/08/180823141038.htm ‘Unexpected’ - Unexpected physical event and unexpected reward or lack of reward (neuronal modeling of external environment) 

—

In my first ten minutes at work I’m exposed to a weeks (month/year/decade) worth of interesting information.  Going from an intellectual tundra to an intellectual rain forest.  

1460 proteins with increased expression in the brain: Human Protein Atlas https://www.proteinatlas.org

Non-profit plasmid repository: https://www.addgene.org 

Protein database: https://www.rcsb.org/3d-view/3WLC/1

Started to think at the molecular level.   

“MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKTGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIR

HNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK

GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDF

FKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSL

FDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKGSYRDTEEEIREAFGVFDKDGNG

YISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK” - CCaMP6m amino acid code. 

  8/31/18 - (Friday) @12:00 in Meeting Room 25.08

GET USB ! ! 

[Lisboa Cultura na ru, Lisbon on the streets Com’Out Lisbon - Katie Gurrerirra ]

MatLab -> Chronux Neural Analysis 

SEPTEMBER 14th!

Week of August 27th, 2018

“Conserved computational circuitry, perhaps taking different arguments on different locations of Basil Ganglia” - Tuesday 

Andrew Barto: http://www-all.cs.umass.edu/~barto/

Basil Ganglia Labs

Okihide Hikosaka Lab: https://irp.nih.gov/pi/okihide-hikosaka

Wilbrecht Lab

Uchida N.  (ubiquitous dopamine motivation and reward) 

Peter J. Magill

Schultz (Pioneer in the field)

C. Savio Chan 

Doya, K. (theory) 

Calabresi, P. (muscarinic) 

Ana Graybiel (McGovern) 

James C. Houk (1994 - Book on Models of Computation in the basal Ganglia)

Evolutionary Conservation of Basil Ganglia type action-selection mechanisms: 

https://www.sciencedirect.com/science/article/pii/S0960982211005288

Dopamine D1 - Retinal Signaling https://www.physiology.org/doi/full/10.1152/jn.00855.2017 [Note to self: Too Off Track]

[ ~ ] Flurorphore Library

Official Badge? [  ] Printer Access [  ]?

—

Online Course on Laboratory Animal Science 

Monday  : 11 [x] 12 [x] 

Tuesday : 13 [x] 14 [x] 

Wednesday: 15 [x] 16 [/] 

Thursday: 17 [x] 18 [x]

Friday: 19 [x] 20  [/] 

Lesson 11 - Behavior and Environment, animals must be housed in an environment enriched to maximize their welfare. 

Lesson 12 - Rodent and Lagomorph Accommodation and Housing - A more comprehensive guide from the macro environment, facilities i.e. establishments, to the micro environments.  Covers health and safety procedures for personnel as well as geometry of housing units (rounded edges to prevent water accumulation).  Absolutely essential.  

Lesson 13 - Collecting Samples and Administrating Procedures - covers the most common collection techniques and materials collected and stressed the importance of doing as little harm as possible to the animal.  

Lesson 14 - Transporting the Animal : Shipper holds most of the responsibility.  Major goals are making sure the journey is as stress free as possible, contingency plans are in place, and that all of the logistics have been carefully planned, communicated, and coordinated between various parties responsible in the shipping.  Also, animals should be prepared mentally and physically for the journey and should have a period of post-transportation to adjust to the new surroundings and environment.  A number of practical issues must be considered such as temperature, availability of food, and access to animals during the journey.  Boxes should be properly labelled in whatever languages are necessary. 

Lesson 15 - The purpose of feeding and nutrition is to meet the energy needs of the animals, which vary by species, physiological state of animal (growth, maintenance, gestation, and lactation).  A number of category of diets exist as well as a variety of specific diets to best fits the needs of the experiment.  This chapter covers particulars of nutrition requirements and stresses the importance of avoiding obesity and malnutrition.  

Lesson 16 - Anatomy and Physiology of Teleosts (Skip for now: Focus on Rodents and Lagomorphs)

Lesson 17 - Anatomy and Physiology of Rodents and Lagomorphs - General characteristics of the anatomy and physiology of six species, 5 rodents and 1 lagomorph.  Mice, rats, guinea pigs, gerbils, and hamsters.  Rabbits.  It covers particularities of each species and has a quiz asking specific facts, mostly centered on commonalities and distinguishing factors.  Worth a close read.  

Lesson 18 - Anaesthesia and Analgesia in Rodents and Lagomorphs . Pre anaesthesia techniques, drug combinations, and repeated warning of the importance of choosing the right drugs and technique for the species.  Use of a chamber.  Methods of anesthesia (IP, IV, Volatile).  Endotracheal Intubation for rabbits; the proper use and administration of analgesics; monitoring during the operation (for example - the paw pain reflex disappears in medium to deep anesthesia 

Lesson 19 - Animal Welfare and Signs of Disturbance - This chapter repeatedly stresses the importance of the relationship between the caretaker and the animal.  It repeats the ideal social, environmental, and nutritional environments for rodents and rabbits and highlights peculiarities of each species.   After reading this one should be better suited to detecting stress, disease, or other ailments in a laboratory animal.  

Lesson 20 - Fish Psychology and Welfare (Skip for now: Focus on Rodents and Lagomorphs) 

Lessons 5, 17, and 20 pertain to fish 

TEST SEPTEMBER 14th 

—

MIT Open Course Ware:

Linear Algebra 

Lecture 2 [/ ] -> Elimination by Matrices, production of elementary matrices, basic computations, and a review of row and column approaches to systems of equations.  Introduction to the basic application of the rule of association in linear algebra.  

Lecture 3 [ ]

Mouse Neuroanatomy 

Ink Figure 16 [x]

Figure 20 [x]

Figure 24 [  ]

Introduction to MatLab:  https://www.youtube.com/watch?v=T_ekAD7U-wU [  ] 

Math Big Picture: Review Single Variable Calculus!  Find reasonable Statistics and Probability Course (Statistical Thinking and Data Analysis?  Introduction to Probability and Statistics?) Mine as well review algebra well I’m at it eh.  

Breathe in.  Breathe out.  

—

Data analysis :: Behavioral Analysis 

—

Ana Margarida - Lecture 6 - Handling Mice techniques 

EuroCircuit can make a piece.  Commercial v. DYI version of products.  

Dario is the soldering, hardware expert.  I.E. skilled technician. 

www.dgv.min-agricultura.pt; it is recommended that the entry on Animal Protection and the section on Animals used for experimental purposes be consulted first. 

—

Sir Ronald Fisher, stated in 1938 in regards to this matter that “To consult the statistician after an experiment is finished is often merely to ask him to conduct a post mortem examination. He can perhaps say what the experiment died of”. 

——

Finally, it is time to publish and reveal the results. According to Santiago Ramón y Cajal, scientific writers should govern themselves by the following rules: 

Make sure you have something to say; Find a suitable title and sequence to present your ideas; Say it; Stop once it is said. 

8/21 Goals

Access ->

:: Champalimaud Private Internet [HR]  Printer [HR]

:: Web of Science (?)

:: PubMed (Nature, Journals, etc.?) 

:: 

———

PRIORITY:  Online Course -> Animal Laboratory Sciences PDF’s 

20 total -> 4 a day || I can finish by Friday 

Monday  : 1 [x] 2 [x] 

Tuesday : 3 [x] 4 [x ] 

Wednesday: 5 [x*] 6 [x] 

Thursday: 7 [x* ] 8 [x]es

Friday: 9 [x ] 10 [x ] 

Notes:

Lesson 1 - Philosophical and ethical background and the 3 R’s

Lesson 2 - Euthanasia.   Recommended, adequate, unacceptable.  Physical or chemical.  Chemical - inhalable or injectable.   Paton Lab uses CO2 and cervical dislocation.   

Lecture 3 - Experimental Design.  Return to as a starting point for basic design (randomized samples and blocks) Integrate with “Statistical Thinking and Data Analysis”

Lecture 4 - Legislation.  Memorize specific laws and acts.

Lecture 5 is highly specific for the care and maintenance of Zebrafish

Lecture 6 - Handling of rodents and mice.  A theoretical overview, this material is essentially kinesthetic.  

Lecture 7 - Provides a technically detailed account of how genetic manipulations are done and propagated.   Deserves a ‘printed’ review and vocabulary cross reference.

Lecture 8 - Health and Safety.  Predominantly common sense.   

Lecture 9 - Microbiology - contains an appendix with list of common infections that will be eventually be good to know.

Lesson 10 - Anaesthesia pre and post operation techniques, risks of infections etc. 

// http://ec.europa.eu/environment/chemicals/lab_animals/member_states_stats_reports_en.htm

http://ec.europa.eu/environment/chemicals/lab_animals/news_en.htm -> General European News regarding 

http://www.ahwla.org.uk/site/tutorials/RP/RP01-Title.html -> Recognizing pain in animals 

Week of 8/20/18 To Do:

Tiago/Team -> Whats the most important priority?

Get Arduino Machine working again [?]

Jupiter/Python Notebook Up [ ]

Bruno MatLab Access [… ]

 - Get documents to HR

 - Animal Lab certified?

 - Logistical/Certificate/Etc.  

  - Start discussing personal project: 

    >  (Rat colony) Wet Lab

    > (Machine Learning) Electric Lab

    > Statistics project

  - Reacquaint with Lab Technology/Protocols 

  - Review papers - Engage back with the science 

  - 

Project Print: Screen shots

[  ] collect 

“Do the job.  Do it engaged.   Engage -> Not just execute the best you can, understand the experiment.

Why? Alternative designs?  Control experiments needed to interpret the data?  Positive controls and negative controls?  What do you need to do to get crisp.  Totally engage.  

How it fits into other experiments?  

“Engage with the science as if it were your baby.”

Execute beautifully… Ask --- et. al.  What does ideal execution look like 

Extra time: allocate time.  Technicians : Freedom to do other things, work with other things, other technical things, giving people independent project to carry out.    Project --- has in mind?  Design.   Hands on education of how science works then reading.   Spend time focused on a problem and in the ideal become the world’s foremost expert on whatever ‘mundane’ aspect of what ever problem you are working on.

Computational in the context of a problem.  Learn to use.   Defining “problems I want to solve.”   As an operating scientist, the technology can change very quickly.   Capable of learning, understanding, and applying.  

Answer questions in a robust way.  Thinking of technology in context of problem.   Deep domain knowledge; focus on experimental more than book reading.   

Realistic path -> Research fellow to PhD. program.  Industry…  Strong head’s up to do research.   First-rate OHSU?  Excellent.    IF: Remember that it is narrow, broader with neuroscience as a component.   Biology < > Neurology.   Real neuroscience computational ->

Juxtasuposed: Engineering, CS, A.I., and all that…

Label in broad ways: Molecular, cellular, systems, cognitive, psychology.   Borders are so fuzzy — as to be 

Domain bias.   In general -> other than P.I. protected from funding.  Publication, the life of the business.   Metric of success is the science they publish.    Work that contributes to being an author = more engaged, more independent.   Evolved to an independent project.    

So incredibly broad -> CRISPR, GFP, Optogenetics, with higher level systems problems.   100 years = absurd.   Look back -> Could we have conceived whats going on today.  

Foremost expert on something how-ever limited.  Grow from there.   Grown from a particular expertise.    

Molecular biologist || Do what a 3 year old is taught to do.  How?  How?  How?  How does that work.  Quantum physics.   Ask questions.  Be open.   

Go to seminars  -> Go to every talk.  Take every note.  Primary literature fundamentally different.   Always learn in context.  Don’t dilute too much (ignore title, abstract, discussion).  Look at figures and tables and derive for yourself what they say.   Look for THE FIGURE or THE TABLE that is the crux and look for the control experiment.    Understand the critical assessment, are the facts valid and warranted?  Infinite amount to learn, don’t spread yourself infinitely thin.  “ 

 To Do: Develop Independent Machine Learning Project 

Gain Access to Web of Science 

————

Paton Learning Lab

Personal Learning Goals 

September 1st - December 1st 

Major Goals 

[  ] Read Principles of Neuroscience 5th Edition

[  ] Complete CSS 229 

[  ] Deep read 12 papers (Write summary || Practice peer review)

Administrative

[  ] Reactivate 

[ / ] Figure out Residence Permit/Visa

Lifestyle

[ x ] Purchase commuter bicycle

[ / ] Purchase waterproof computer/messenger bag

Language

[x] …. Focused practice minimum 20 minutes daily …? 

[  ]   Find language partner 

[  ] Portuguese film/television/music 

UPCOMING

Phone conversation with --------

Tuesday, August 7th 9:00 a.m. EST (10:00 a.m.