#ecori

[sophios-draws]

my child ecori restriction enzyme

[finitepeace]

december reads: xiewei x jiang xuening <from story of kunning palace>

The Fantastic Mr. Zhang by ecorie | G, 2k words, post-canon |

Prime Minister Xie Wei is not above sending Zhang Zhe to prison for his "crimes against humanity". His father-in-law Minister Jiang, near retirement but still in full possession of his faculties and impeccable memory, thinks what goes around comes around. Yan Lin thinks he really need to teach his son not to start family drama.

[libraryben]

"Done by Dutch organization Ecorys, the study might have been lost altogether if not for the effort of EU parliamentarian Julia Reda. She submitted a freedom of information request in July 2017, and after stalling twice, the commission finally produced it. The conclusion? "With the exception of recently released blockbusters, there is no evidence to support the idea that online copyright infringement displaces sales," Reda wrote on her blog.

It's not as though the EU just forgot the study in a drawer. It concluded that one specific category, blockbuster movies, is negatively impacted by piracy, with ten downloads leading to about four fewer cinema visits. Overall, that reduced sales for certain films by about 4.4 percent on average. Two EU Commissioners used those results in a 2016 academic paper to bolster claims that piracy impacts cinema ticket sales, digital rights group EDRi noticed.

As for the other industries that rely on copyright (games, books and music), the study found "no robust statistical evidence of displacement of sales by online piracy." In the case of games, it concluded that unauthorized playing might actually make it more likely users will buy them. None of those results ever appeared in any EU Commission academic studies or to the public anywhere else, however."

[mongowheelie]

MAGAs Want to Spread Hate, Fascism and Disease. Don’t Let Them

Source: ecori.org

MAGAs Want to Spread Hate, Fascism and Disease. Don’t Let Them

Source: ecori.org

[factreviver]

DNA RECOMBINATION TECHNOLOGY (Process).

1. Isolation of Genetic Material (DNA)

The first step in creating recombinant DNA is isolating the genetic material. The genetic material (DNA) needs to be extracted from the cell of the organism whose DNA you want to manipulate. This process involves the following:

– **Cell Lysis:** Cells are broken open through physical or chemical methods, releasing the DNA into the solution. Common techniques include using detergents, enzymes, or mechanical disruption to break the cell walls or membranes.

– **Separation of DNA from Other Cellular Components:** Once the cells are lysed, the DNA must be separated from proteins, lipids, and other cellular materials. This is often done using a phenol-chloroform extraction process or commercial DNA extraction kits, which purify DNA from cellular debris.

– **Precipitation of DNA:** After the cellular debris is removed, the DNA is precipitated out of the solution using alcohol (typically ethanol or isopropanol). This helps to concentrate and purify the DNA further, making it suitable for subsequent manipulation.

The isolated DNA serves as the foundation for the entire process of recombinant DNA technology in Biology

2. Cutting of DNA at Specific Locations | Biology

Once the genetic material has been isolated, the next step is to cut the DNA at specific sites. This is done using **restriction enzymes**—molecular “scissors” that recognize specific sequences in the DNA and make cuts at these points.

– **Selection of Restriction Enzymes:** The choice of restriction enzyme is crucial. Each enzyme recognizes a specific short sequence of DNA (usually 4-8 base pairs) called a recognition site. For example, the enzyme EcoRI cuts the DNA at the sequence GAATTC. Scientists select enzymes based on where they need to cut the DNA for their experiment.

– **Digestion of DNA:** The DNA sample is incubated with the chosen restriction enzyme under specific conditions (e.g., temperature, pH). The enzyme recognizes its target sequence and cuts the DNA, creating fragments with either blunt ends or “sticky ends” (short single-stranded overhangs that can anneal to complementary sequences).

These cut fragments form the basis for the recombinant DNA assembly. The next step is selecting the desired fragment for insertion into a host organism. WANT TO KNOW MORE?

3. Isolation of the Desired DNA Fragment | Biology

Once the DNA is cut, the mixture contains a variety of DNA fragments of different sizes. To create recombinant DNA, the specific DNA fragment containing the gene of interest must be isolated. This is done through a process called **gel electrophoresis**.

– **Gel Electrophoresis:** The cut DNA fragments are loaded into a gel made from agarose (a gelatin-like substance), which is placed in an electric field. The DNA fragments are negatively charged due to the phosphate groups in their backbone, so they migrate toward the positive end of the gel when the current is applied.

– **Separation by Size:** Smaller DNA fragments move more quickly through the gel, while larger fragments move more slowly. This creates bands of DNA that can be visualized using a DNA stain (such as ethidium bromide) under UV light.

– **Extraction of the Desired Fragment:** The desired fragment is identified by its size, excised from the gel using a sterile scalpel, and purified. Several methods can be used to purify the DNA fragment from the gel, such as gel extraction kits or spin column purification methods.

The isolated DNA fragment, now purified, is ready for amplification and further manipulation.

 4. Amplification of Genes Using PCR                                    (FACTZONE.IN)

If more copies of the desired DNA fragment are needed, the next step is amplification through the **polymerase chain reaction (PCR)**. PCR is a technique that can generate millions of copies of a specific DNA sequence in a short amount of time.

– **Denaturation:** The DNA fragment is heated to around 94-96°C to separate the two strands of the DNA double helix.

– **Annealing:** The temperature is lowered (typically between 50-65°C) to allow short single-stranded DNA primers to bind (anneal) to their complementary sequences on the target DNA.

– **Extension:** The temperature is raised to around 72°C, which allows the enzyme **Taq polymerase** to synthesize new DNA strands by adding nucleotides to the primers, extending them to create a complete copy of the target DNA sequence.

This cycle of denaturation, annealing, and extension is repeated about 25-35 times, leading to the exponential amplification of the target DNA sequence.

5. Ligation of the DNA Fragment into a Vector

Now that the desired DNA fragment has been amplified, the next step is to insert it into a **vector**—a carrier that will help introduce the DNA into a host organism. The most common vectors are plasmids, small circular pieces of DNA that can replicate independently within a host cell.

– **Preparation of the Vector:** The plasmid vector is also cut with the same restriction enzyme used to cut the DNA fragment. This ensures that the ends of the vector and the DNA fragment are complementary, allowing them to anneal.

– **Ligation:** The DNA fragment is mixed with the cut plasmid vector and an enzyme called **DNA ligase**. DNA ligase joins the sticky ends of the DNA fragment and the vector, forming a continuous, stable piece of DNA known as a recombinant plasmid.

The recombinant plasmid now contains the gene of interest, along with other features (such as antibiotic resistance genes) that will allow for the selection of successful recombinants in the next step.

6. Insertion of the Recombinant DNA into the Host Cell

With the recombinant plasmid ready, the next step is introducing this plasmid into a host organism. The most common hosts are **bacterial cells**, such as *E. coli*, but yeast, plant, or animal cells can also be used.

– **Transformation:** The process of introducing the recombinant plasmid into the host cell is called transformation. In bacterial cells, this is often achieved by treating the cells with chemicals (such as calcium chloride) or exposing them to an electric field (a process known as electroporation). These treatments temporarily make the bacterial membrane more permeable to the plasmid DNA.

– **Selection:** Once the bacteria have been transformed, they are grown on agar plates containing antibiotics. Only the bacteria that have successfully taken up the plasmid (which contains an antibiotic resistance gene) will survive and grow. This allows researchers to easily identify the colonies that contain the recombinant DNA.

7. Culturing the Host Cells

The transformed host cells (usually bacteria) that have taken up the recombinant plasmid are then cultured in a nutrient-rich medium. This process allows the cells to multiply rapidly, creating millions of copies of the recombinant DNA within a short period.

As the bacteria divide, they replicate the recombinant plasmid along with their own DNA, ensuring that the foreign gene is passed on to each new generation of cells. This process is referred to as **cloning** of the DNA fragment.

8. Extraction of the Desired Gene Product

Once the host cells have been successfully cultured, the recombinant DNA can be extracted, or the gene product (such as a protein) can be harvested.

– **DNA Extraction:** The recombinant plasmids can be extracted from the host cells using plasmid isolation techniques, such as the alkaline lysis method. This purified recombinant DNA can then be used for further research or applications.

– **Protein Expression:** If the goal is to produce a protein, the host cells can be induced to express the gene of interest, leading to the production of the recombinant protein. This protein can then be extracted and purified using techniques like centrifugation, affinity chromatography, or electrophoresis.

9. Downstream Processing

The final step in recombinant DNA technology is **downstream processing**, which involves purifying and characterizing the desired product, whether it is DNA or protein.

– **Purification:** If the goal is to produce a protein, the recombinant protein must be purified from the host cell lysate. This is done using methods like chromatography or filtration, which separate the protein of interest from other cellular proteins and impurities.

– **Characterization and Quality Control:** The purified product is then characterized to ensure that it is functional and meets the desired specifications. This may involve determining the DNA or protein sequence, measuring protein activity, or confirming the presence of any modifications.

– **Packaging and Storage:** Finally, the purified product is packaged and stored under suitable conditions, ensuring its stability and readiness for use in various applications, such as gene therapy, vaccine production, or industrial biotechnology through the help of Biology.

INTERESTING FACTS AND INFORMATION ON MORE TOPICS. CLICK HERE.

[moniquill]

They're also a protected flower here in RI, along with Lady's Sippers:

[superstarpixelations]

Plague meister

[csesolar]

#MLPE NAO É TUDO IGUAL #ECORI #APSYSTEM #CSESOLAR https://www.instagram.com/p/CUsq0nOJZxX/?utm_medium=tumblr

[drwcn]

*Navigation*

~ESTABLISHED FICS~

{Begotten} — completed, on AO3.

{Alone Stands the Quiet} — completed, Begotten’s sequel, on AO3

{The Good Arion} — completed, on AO3. 

{The Shadow Beneath the Light} — completed, #tsbl, on AO3

~~~

{Before the Sun Falls} —  navigation page or AO3 :)

{Discordance} — navigation page or AO3. :) 

~FICLET SERIES~

#cql ficlet

{Deep Waters} — completed, dark!lxc, master list

{A Willing Hostage} — completed, nmj bridenaps jyl, master list

{Remnant} — completed, dark!lan brothers au #2, master list

{Midnight Sun} — completed, fem!wwx + wangxian kid, master list

{The Red Regent} — completed, evil!wen qing au, master list

{Without Envy} — completed, concubine/spy!wwx & prince!lwj, master list

~~~

{Courtship & Cultivation} — p&p x the untamed, #ppcql

{Carbon in the Steel} —dark!lan brothers au #1, master list

~TAGS~

cql reference - information about cdrama world building (fashion, language, culture etc). 

~ Corie :)

[just-like-playing-tag]

Emma, Norman and Ray - Chapter 153: Coward

[mdzs-fic]

Additional Tags:

Family Drama, Family Feels, Post Siege of Nightless City, Angst and Feels, Alternate Universe - Canon Divergence, Not really divergence, more like filling in the blanks, Lan Zhan being a good dad, Xichen being a good uncle, Jiang Cheng showed restraint for once, All the uncles will show up in this fic at some point, even Jin Guangyao, This kid has too many uncles than is good for him, the 13 years in between, Jiggy has shown up in chapter 4, he is still a terrible person but we write complex character in this joint, no smut this is a character study fic my dudes Series: Part 1 of Foundling

Word count: 37k

Summary:

“He’s mine.” He echoed what had once been teasingly said in jest, and added, “This is my son.”

Against all odds and without a choice, Lan Zhan brings A-Yuan back to Cloud Recesses. Xichen keeps his brother’s secrets, and shields the child when Lan Zhan could not.

[enalfersa]

VOX El Escorial exige reforzar la Policía Local ante el abandono del PP y PSOE

VOX El Escorial exige reforzar la Policía Local ante el abandono del PP y PSOE

“Sin seguridad no hay libertad”, ha recordado el portavoz de VOX, Emigdio Mª López, que insistirá en volver a proponer medidas de mejora para los agentes locales que ya fueron aprobadas pero ninguneadas por el Gobierno Municipal. El Escorial, 29 de marzo de 2022.– VOX El Escorial ha mostrado su preocupación por la seguridad, una de las bases de la libertad de los ciudadanos. Por ello, ha…

[wangxianficrecs]

have u read begotten 😭😭😭

Ohmygod you bastard

I just read it, and I’ve been CRYING, thanks so much for that!

Begotten

by ecorie G, 29k WIP (obviously I read it anyway, and I’m glad I did, but it hasn’t been very long so probably Author will finish the final chapter.)