#basal media in plant tissue culture

[kosheeka]

Basal Media Uses

Researchers have done some studies to choose the correct form of basal media and supplementation in form of culturing primary cells with company-composed specialty media and DMEM/F12, as this is a good supportive basal medium, for comparing the growth and maintenance of primary cells. Moreover, some studies also compared this medium with higher serum levels (10%) versus lower serum levels (5%) in combination with endothelial cell growth supplements, hormones, and growth factors for the optimized growth of endothelial cells.

Studies showed that primary cells grew more quickly with the formulation having 5% serum as compared to 10%, thus suggesting that a lower serum formulation, in combination with growth factors and hormones, is better for primary endothelial cell proliferation.