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My OCs! They're Miriam, Mary and Mireia, a trio of secret agents who work in the DPB, Europe's most prestigious secret agency
#dpb#dont play brave#dark phantom brigade#dpb miriam#dpb mary#dpb mireia#oc#oc art#original character
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DPB - "I Feel So Good Today" (Happy Mix) (@worldofdpb)
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DPDP’s New Data Breach Reporting Rules: A Comprehensive Guide for Businesses
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The specter of data breaches looms large in the digital age, casting a shadow over user trust and platform accountability. In India, the impending Digital Personal Data Protection (DPDP) Act promises a paradigm shift in data governance, with stringent regulations designed to safeguard user privacy and empower individuals. Among its most crucial provisions lies the mandate for immediate data breach disclosure to the Data Protection Board (DPB). This article delves deep into the intricacies of this requirement, dissecting its implications for platforms, users, and the broader data ecosystem.
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D-PBS And Its Role In Cell-Cell Interactions
Dulbecco’s Phosphate Buffered Saline (D-PBS) is an essential component in cell culture and research laboratories across the globe. To the casual observer, this may seem like simply a rinsing solution, but it maintains physiological conditions and facilitates important cell-cell interactions.
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Understanding D-PBS Composition
D-PBS composition has a very balanced salt solution especially constructed to emulate the physiological environment of mammalian cells. These are the main ingredients:
● Sodium chloride (NaCl): Osmotic balance, essential ions.
● Potassium chloride (KCl): Proper ion concentrations for cell function.
Sodium phosphate and potassium phosphate: Buffers with stable pH critical for optimal cell growth and activity.
Absence of Calcium and Magnesium
The important difference between D-PBS and standard PBS is the absence of calcium and magnesium ions. They are vital for many cellular processes, but they hamper certain experimental procedures:
● Cell dissociation: Calcium and magnesium ions encourage cell-cell adhesion. This is one factor that makes efficient dissociation of cells from culture surfaces or tissues problematic.
● Immunological studies: These ions may interfere with antibody binding, affecting the validity of immunostaining and flow cytometry experiments.
D-PBS and Cell-Cell Interactions
There are several ways in which D-PBS directly affects cell-cell interactions:
● Washing away growth factors and cytokines: These extracellular signaling molecules are known to influence different aspects of cell behavior-including proliferation, differentiation, and migration-when washed out using D-PBS potentially provide the possibility of being more controlled over experimental conditions.
● Cell viability maintenance: A physiologically balanced surrounding created by D-PBS minimizes osmotic stress and protects cell viability during washing and handling.
D-PBS Applications in Cell-Cell Interaction Studies
The crucial role played by D-PBS in all these various experimental settings with respect to cell-cell interactions:
● Co-culture experiments: Preparation of washed cells to be co-cultured with another cell type ensures that residuals do not affect the co-culture.
● Cell migration assays: D-PBS suspension of cells will be prepared and then produced in concentration-defined gradients of chemotactic substances, which will allow cells to migrate through chemotaxis.
● Adhesion studies: D-PBS washes both the cells and substrates before assays in order to prevent any potential altering of attachment of the cells due to residual proteins or factors.
Conclusion
D-PBS regulates cell-cell interactions and, despite its plain and simple composition, offers many aspects in governing cell-cell interactions. Delbecco’s PBS perhaps holds a spectrum in a microscopically changed ionic environment and neutral influence of divalent cations on any cell-based experiment, starting with simple cell culture to the most integrated studies of cell communication and behavior. Visit PurMa Biologics LLC to get more information.
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The latest technologies, Pass Boxes (SPB & DPB) from InnovativePPL, can help you improve your cleanroom performance! Our solutions transmit materials safely and efficiently, ensuring optimum efficiency and safety. Professionals trust them in supervised environments and labs. Visit us at http://innovativeppl.com/pass-box-spb-dpb.html or +91 9824243397 for more details.
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Thomann Cyberweek 2023 - Up to 60% of for 10 Days
Thomann Cyberweek 2023 runs from 17 – 23 and offers discounts of up to 60% off on a huge range of products. This sale is way better than just a regular Black Friday deal and includes major brands like Fender, Gibson, and Neural DSP in the deals. Up to 60% Off The annual Thomann Cyberweek has just started today and runs for 10 days straight with up to 60% off on gear purchased on the site. If you…
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#Black Friday#Bugera 333XL Infinium#Bugera V22HD Infinium#Cort GAMY-BV Natural#Diezel VH4 Pedal#Discount#Effects 60% off#ESP LTD TE-1000ET Koa Natural#Fender#Fender Tele Custom HS ACFM Relic#Gibson#Gibson Les Paul Standard 60s SB#Gibson LP 57 Black Beauty VOS#Gretsch G6128T-57 VS Duo Jet CG#Guitars#Harley Benton MR-Modern CAR#Line 6#Marshall Origin 20H Head#Maybach Motone J 3-Tone Aged Custom#MXR The Duke of Tone#Neural DSP Quad Cortex#Nux XTC OD#Squier Contemp Strat Special DPB#Squier Paranormal Offset Tele BK#Squier Tele 40th Anniv VB#tc electronic Hall of Fame 2#Thomann Cyberweek 2023
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Remember these? The investment of the future! Beanie Babies!
💲
The sign says $1.00
#Beanie Babies#Beanies#flea market#flea market finds#investment#DPB Farmers Market#Delta PA#Whiteford MD
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I know your box is on hiatus rn but I just wanted to know if you’re doing alright 😊 I just seriously love your work and wanted to know if your okay since you haven’t uploaded anything since a while ago. I AM NOT ASKING YOU TO RUSH THINGS TO GET CONTENT OUT THOUGH! 😭 I just wanted to know what you’re up to 💕💕
Lots of love,
tako_taki
Hi! I'm sorry for not getting to this when you sent it in, and yeah, I'm somewhat okay? things have just been pretty hectic; I'm no longer going to college since I had to drop out due to my mental health and financial situation. I've struggle with a lot of mental health issues, such as DPB(Dependant Personality Disorder),Depression, PTSD, ADHD, and BPD(Borderline Personality Disorder)*All disorders I have been properly diagnosed with from doctors*. Due to this I've been struggling to hold a job as well so that's been pretty taxing on my mental health as well. Currently my therapist is wanting me to file for some type of disability but with the American health system it can be quite hard if the government isn't convinced. I do plan to start writing again don't worry.
I will admit I have been debating the thought if on if I should continue with this blog or not. But after heavy consideration, I have decided to continue with it, I love to write, and I love kpop. So, I will proceed to opening up my request again. I have also even considered on openly talking about mental issues, so like if anyone has any questions or simply wants to talk about said topics I am here.
Again, I'm sorry for not posting and pretty much ghosting y'all, I love all of you guys and I really appreciate the support and love from all of y'all.
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Hi it’s me drift I’m gonna be emotional about a band called The Maine again sorry
So usually when TM is on tour I just get to enjoy everyone sharing videos and the fun antics and bits we love and have come to expect (John saying IM THIRTY FIVE AND DONT KNOW WHAT TIKTOK IS every night, for example). And I love it and I love seeing how much my friends gush over our band afterwards
BUT ON THIS TOUR I instead! Get to see people find them for the first time!! And go “I have never heard of this band but they were so fun!” Or “THIS WAS SO CHAOTIC AND ENTERTAINING WHO ARE THESE GUYS!!” Or like I’ll watch themaineontour ‘s nightly videos (my friend started this a couple years ago where it’s basically a traveling Instagram account, somebody who’s going to the show basically posts stories direct to it and it rotates thru the tour so you get to feel like you’re there, it’s my fave) and you’ll see people around them start the set sitting down but by the time DPB or certainly LYAL is playing they are daaaaancing!
And it is SO INCREDIBLE ATHAT *EVERY SINGLE NIGHT* I get to watch people fall in love with my band. It makes my heart so full. My entire heart. Just so happy.
Thank you FOB 🥹🥹 thank you 2ourdust
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Explore The Benefits Of D-PBS For Your Scientific Applications
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17.01.25
We went for a walk at the Devils Punchbowl in Hindhead today.
Our last visit to the DPB was three years ago!
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Voleva fare chiarezza, la premier Giorgia Meloni. Per ricondurre a verità, ovviamente via Social, le “molte falsità” che starebbero mettendo in discussione, a suo avviso, il “record della storia d’Italia” di fondi stanziati per la Sanità. Un tentativo, però, che ha finito per confondere ulteriormente le acque nel balletto di cifre che, ormai da 48 ore, sta andando in scena sui numeri della Manovra e, in particolare, su quelli relativi al capitolo salute.
La confusione, secondo il presidente della Fondazione Gimbe, Nino Cartabellotta, è presto spiegata: “Lei somma le risorse assegnate alla sanità in due Leggi di Bilancio: 2024 e 2025. In attesa del testo della manovra, stando al Dpb i numeri sono: +0,86 miliardi di euro nel 2025 ; +3,1 miliardi nel 2026; +0,17 miliardi nel 2027” (leggi articolo a pagina 2). Cifre accompagnate da un invito rivolto alla premier: “Lasci stare i record, quelli appartengono allo sport – conclude Cartabellotta -. Altrimenti citiamo come triste primato i 4,5 milioni di persone che non si curano più, di cui 2,5 milioni per ragioni economiche”.
Ma in fatto di record, a ben vedere, ce n’è sicuramente uno che il governo Meloni può vantare senza timore di smentite. Ed è il numero di minori e di famiglie di operai in povertà assoluta registrati nel 2023. I primi hanno toccato la cifra di 1,29 milioni, vale a dire il 13,8% del totale dei minorenni italiani. Le seconde, invece, hanno fatto registrare una crescita consistente passando dal 14,7 al 16,5% (leggi articolo a pagina 3). I numeri non mentono. E anche quando si prova a mischiare le carte scambiando l’ordine degli addendi, il totale non cambia. Mai.
I numeri non mentono - A. Pitoni su La Notizia
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SMKN 1 Grogol: Pusat Pendidikan Kejuruan Berbasis Teknologi dan Agribisnis
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SMKN 1 Grogol merupakan salah satu Sekolah Menengah Kejuruan (SMK) yang terletak di Kabupaten Kediri, Jawa Timur. Sekolah ini berfokus pada pengembangan keterampilan dan pengetahuan siswa di berbagai bidang yang relevan dengan kebutuhan industri dan pasar kerja. Dengan berbagai jurusan yang ditawarkan, SMKN 1 Grogol siap mencetak lulusan yang kompeten dan siap bersaing dalam dunia kerja. Berikut adalah beberapa jurusan unggulan di SMKN 1 Grogol:
1. Teknik Komputer dan Jaringan (TKJ)
Jurusan Teknik Komputer dan Jaringan memberikan pendidikan di bidang teknologi informasi yang mencakup dasar-dasar perakitan komputer, pengelolaan jaringan, pemrograman, dan keamanan siber. Siswa dibekali dengan kemampuan untuk merancang, membangun, dan mengelola jaringan komputer, baik untuk skala kecil hingga besar.
Keunggulan TKJ di SMKN 1 Grogol:
Laboratorium lengkap dan modern untuk praktik jaringan komputer.
Kerja sama dengan berbagai perusahaan teknologi untuk program magang.
Sertifikasi profesional yang diakui secara nasional dan internasional.
2. Akuntansi dan Keuangan Lembaga (AKL)
Jurusan Akuntansi dan Keuangan Lembaga membekali siswa dengan kemampuan di bidang akuntansi keuangan, pembukuan, perpajakan, serta analisis laporan keuangan. Jurusan ini dirancang untuk mencetak tenaga akuntansi yang handal dan mampu bekerja di berbagai jenis industri, baik sektor publik maupun swasta.
Keunggulan AKL di SMKN 1 Grogol:
Pembelajaran berbasis simulasi akuntansi untuk pengalaman praktis.
Dosen yang berpengalaman dalam industri akuntansi dan keuangan.
Peluang kerja yang luas di perusahaan, lembaga keuangan, maupun BUMN.
3. Teknik Sepeda Motor (TSM)
Jurusan Teknik Sepeda Motor adalah pilihan bagi siswa yang tertarik dengan dunia otomotif, khususnya di bidang perawatan dan perbaikan sepeda motor. Siswa akan diajarkan berbagai keterampilan seperti teknik perbaikan mesin, sistem kelistrikan sepeda motor, serta teknologi terbaru dalam dunia otomotif.
Keunggulan TSM di SMKN 1 Grogol:
Bengkel praktik yang dilengkapi peralatan otomotif modern.
Program magang dengan bengkel-bengkel resmi berbagai merek ternama.
Sertifikasi kompetensi otomotif yang diakui secara nasional.
4. Teknik Kendaraan Ringan Otomotif (TKRO)
Jurusan Teknik Kendaraan Ringan Otomotif fokus pada pembelajaran tentang kendaraan roda empat. Siswa akan belajar tentang perawatan dan perbaikan mobil, sistem mesin, kelistrikan kendaraan, hingga penggunaan teknologi otomotif terkini.
Keunggulan TKRO di SMKN 1 Grogol:
Akses ke peralatan diagnostik modern yang digunakan di industri otomotif.
Kurikulum yang selalu diperbarui sesuai dengan perkembangan teknologi otomotif.
Kerja sama dengan dealer mobil resmi untuk praktik lapangan.
5. Desain dan Permodelan Busana (DPB)
Jurusan Desain dan Permodelan Busana membekali siswa dengan keterampilan dalam mendesain, membuat pola, dan merancang busana. Siswa juga diajarkan teknik menjahit dan menyelesaikan pakaian, serta bagaimana menghasilkan produk busana yang kreatif dan fungsional.
Keunggulan DPB di SMKN 1 Grogol:
Studio mode yang lengkap dengan peralatan desain dan jahit.
Peluang untuk mengikuti lomba desain dan pameran busana.
Pembelajaran tentang industri mode dan tren fesyen terbaru.
6. Agribisnis Pengolahan Hasil Pertanian (APHP)
Jurusan Agribisnis Pengolahan Hasil Pertanian memfokuskan pendidikan pada pengolahan hasil pertanian menjadi produk yang bernilai jual tinggi. Siswa akan belajar proses pengolahan, pengemasan, hingga pemasaran produk hasil pertanian.
Keunggulan APHP di SMKN 1 Grogol:
Fasilitas laboratorium yang mendukung praktik pengolahan hasil pertanian.
Kunjungan industri dan kerja sama dengan perusahaan pengolahan hasil pertanian.
Pelatihan kewirausahaan dalam pengelolaan agribisnis.
Fasilitas dan Ekstrakurikuler
Selain menawarkan berbagai jurusan yang menarik, SMKN 1 Grogol juga menyediakan fasilitas penunjang seperti laboratorium komputer, bengkel otomotif, studio busana, serta laboratorium pertanian. Sekolah ini juga mendukung pengembangan minat dan bakat siswa melalui berbagai ekstrakurikuler seperti pramuka, olahraga, seni, hingga organisasi siswa.
Kesempatan Kerja dan Lanjut Pendidikan
Lulusan SMKN 1 Grogol memiliki peluang kerja yang luas di berbagai industri, baik di sektor teknologi, keuangan, otomotif, agribisnis, maupun mode. Selain itu, lulusan juga didorong untuk melanjutkan pendidikan ke perguruan tinggi atau menjadi wirausahawan di bidang keahlian mereka masing-masing.
Dengan beragam jurusan dan dukungan fasilitas yang cukup memadai, SMKN 1 Grogol dapat dijadikan pilihan ideal bagi siswa yang ingin mengembangkan keterampilan praktis dan siap menghadapi dunia kerja.
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Daraya's Punk Band is back in action! Featuring a whole new Chixie voice!
(I hope you guys like this, it took forever to put together since I forgot how to do keyframe animations in the time between this video and the DPB musical lol)
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Up to episode 12 of campaign 3 and I love that we're at the point of CR in jokes where I can see "DPB" two minutes ahead of where I am in Flando's timestamps and I'm already like 😏
#that was one of my favourite jokes last campaign#because it was one of those times Liam just says something SO WEIRD that you have to go with it#... and there it is. of course it was a Sam joke 😅#we'll have lost a lot of that joke from last campaign actually because a lot of it was on Talks#critical role#liam o'brien
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