#DDNP

[johnnyslittleanimalblog]

Staring Contest by Doug Dance Via Flickr: The love between a mother and her cub is unmistakable. Riding Mountain National Park, Manitoba, Canada

[coffeenuts]

[phregnancy]

ddnp gonna post a pic them kissing before the bed💑💑💑

you think they.. you think they k.. that they ki🤢🤢🤮🤮🤮🤮🤮🤮🤮

[randomluck-ofthe-universe]

DDNP anon.

thank you very much for your info.

😑😑

[megadilf]

LOL dan just turned 30 and is already settled down with a house what a NERD

[szabadpecsofficial]

Az ég kék, a beruházások drágulnak: most egy szaporcai turistaház 120 millióval

Az ég kék, a beruházások drágulnak: most egy szaporcai turistaház 120 millióval

A legfrissebb Magyar Közlöny szerint a kormány újabb költségvetési támogatást nyújt az Ős-Dráva Látogatóközpont mellé tervezett 50 férőhelyes turistaházhoz.

Ezzel már több mint 400 millió forintos költségvetésnél tarthat a Duna-Dráva Nemzeti Parkhoz (DDNP) tartozó projekt. Az eredeti tervek szerint 2018 végére kellett volna készen lennie.

A közbeszerzési dokumentumokból – amiknek a nyilvá…

[meetthebreedloves]

My 6 ❤️❤️❤️❤️❤️❤️ Justa a day at the #Beach Mommy & The Kids #meetthebreedloves #kids #family #playing #love (at Beaches, Jacksonville Beach, Florida) https://www.instagram.com/meetthebreedloves/p/Bvaa02-DdNP/?utm_source=ig_tumblr_share&igshid=lnbr6udo7m6r

[rnomics]

Molecules, Vol. 22, Pages 86: Novel PEI/Poly-γ-Gutamic Acid Nanoparticles for High Efficient #siRNA and Plasmid DNA Co-Delivery

The efficient delivery of sufficient amounts of nucleic acids into target cells is critical for successful gene therapy and gene knockdown. The DNA/#siRNA co-delivery system has been considered a promising approach for #cancer therapy to simultaneously express and inhibit tumor suppressor genes and overexpressed oncogenes, respectively, triggering synergistic anti-#cancer effects. Polyethylenimine (PEI) has been identified as an efficient non-viral vector for transgene expression. In this study, we created a very high efficient DNA/#siRNA co-delivery system by incorporating a negatively-charged poly-γ-glutamic acid (γ-PGA) into PEI/nucleic acid complexes. Spherical nanoparticles with about 200 nm diameter were formed by mixing PEI/plasmid DNA/#siRNA/γ-PGA (dual delivery nanoparticles; DDNPs) with specific ratio (N/P/C ratio) and the particles present positive surface charge under all manufacturing conditions. The gel retardation assay shows both nucleic acids were effectively condensed by PEI, even at low N/P ratios. The PEI-based DDNPs reveal excellent DNA/#siRNA transfection efficiency in the human hepatoma cell line (Hep 3B) by simultaneously providing high transgene expression efficiency and high #siRNA silencing effect. The results indicated that DDNP can be an effective tool for gene therapy against hepatoma. http://bit.ly/2j88gjz #MDPI

[johnnyslittleanimalblog]

Head to Head by Doug Dance Via Flickr: Yellowstone National Park, WY June 2011 Photographed in 'Little America'.

[dancedancenoise]

[johnnyslittleanimalblog]

Head to Head by Doug Dance Via Flickr: Yellowstone National Park, WY 

[randyrphotos]

Animals | Trio of Swans

Animals | Trio of Swans

Please use Searchy.One Description From Photographer if Any: By grayskies369 Source: 500px.com

[dancedancenoise]

[fulviomeloni]

Canary Hot Spring by grayskies369 http://ift.tt/1HoRm4B #travel #nature #500px