#sub!coquelic

[sporadic-gremlin]

Feeling quite moody today after having exams… all I want to do is to fuck and break coquelic, absolutely destroy her till she’s babbling nonsense and wanting more. And doing it in front of the other Garden members? Fuck yeah.

"god, you feel so—" your words are cut off with a moan, head involuntarily tilting forward as coquelic's pussy clenches around your cock.

coquelic arches off the bed, a whimper squeezing its way past her lips. her skin was flushed and damp but right now, she was nothing if not the image of divinity. those iridescent ruby eyes snapped shut as another orgasm tore through her, tears beading in the corners of her eyes. even as she came, you fucked her through it, folding her into a mating press.

"yes, right there!..." coquelic choked. you saw the way her chested heaved as she tried her best to catch her breath before you fucked it back out of her.

"fuck, 'm gonna cum," you groan, gritting your teeth as waves of euphoria took a hold of you, cock throbbing as ropes of warm cum filled coqeulic’s pussy. “do you think she deserves another, garofano?”

garofano hummed and narrowed her eyes at her mentor. coquelic has always been gorgeous but there’s just something about seeing her in this state that really captured her essence… “i think she does.”

you have a dopey smile to garofano before pulling coquelic’s legs over your shoulders, pressing sloppy kisses to the inside of her thigh. fuck, you were so sensitive but coquelic looked so pretty like this. it felt like coquelic was trying to push you out with the way she was pulsing around you but as you rolled your hips, your cock made its home deep inside her warmth.

“ohgodfuck, ‘m so close, ‘m gonna c—fuck!—cum,” coquelic sobbed, gripping at garofano’s dress. coquelic gasped and panted, trying to catch her breath as her orgasm ripped it from her.

you moaned, thrusting into her a little bit harder than before. “that’s it, baby. fuck, you feel so good around me.”

garofano blushed a little at your words. you were both so lost in each other that you must’ve forgotten that there were others in the room with you…

[vrachis]

ik coquelic is broke asf canonically but tell me she’d be a good sugar mama in an au where she’s rich 🤤🤤🤤.

(her not caring what her sugar baby buys but when reader shows up w a newly bought dildo that they want to try out- :3)

[sinsmockingbird]

SUCCUBUS!COQUELIC | Event

PAIRING: Coquelic x Afab!Reader

WARNINGS: Smut, NSFW, Sub!Reader, Dom!Character, Succubus!Coquelic, Human!Reader, Monster Fucking, Lingerie, Cunnilingus

AUTHORS NOTE: Finally the first part is here! Enjoy a relatively mild monster fucking fic to ease us into the event. Note; Reader is one of Coquelic's concubine.

IT WASN'T uncommon for Coquelic to return to the Garden quite... hungry. You've learned by this point to always be ready for her when she returns, knowing just how much she's craving some delicious essence.

Making sure that her room was tidy and that everything was set correctly, you moved your hands down your body to fix up the revealing lingerie you were wearing, into place. Then you glanced into the mirror inside her room and made sure your hair was neat (it wouldn't be in a little bit).

After making sure you looked good, you glanced over at the clock and noted that you only had a few minutes left. So quickly you settled yourself onto Coquelic's bed and laid down, but not before tossing some rose petals around you and making sure that the lingerie was revealing more than it already was before settling down and waiting.

And you didn't have to wait long.

When a portal opened up in the room, your heart quickly began to rapidly hammer in your chest. You watched as Coquelic came storming through, a scowl adorning her beautiful face. You noted the dark red horns sprouting and curving from her head, red wings briefly fluttering in aggravation, and then finally the slender tail swishing back and forth behind her.

You watched as Coquelic froze as soon as her eyes landed on you, slowly blinking them a few times before a wide grin spread across her lips as her eyes looked you over. "My my, what's this, my little rosebud?"

"U-Uhm, a gift... a gift for you," You answered, feeling your face flush red as you felt her eyes trailing over every inch of your body. "I- I thought you would be h-hungry upon your return."

"Hm, you thought correctly. As always," Coquelic mused as she moved towards you and crawled onto the bed, her tail swishing back and forth behind her in a tantalizing way. "You'll still let me do anything to you, correct?"

"C-Correct," You meekly nodded your head, obediently moving to lay flat on the bed on your back, allowing her to straddle your waist.

"Mm, good," Coquelic licked her her lips in hunger, before diving down towards you.

You gasped as her lips crashed against yours, your teeth hitting against each other as she shoved her tongue into your mouth. You put up no fight, surrendering yourself to Coquelic, moaning at the feeling of her long tongue snake out and swipe across every inch, before almost shoving itself down your throat. You choked for a moment as she stuck her tongue momentarily down your throat, before gasping for air as she pulled away.

She left open mouthed kisses down your jaw and neck, slowly but surely moving down your body, not caring how messy her kisses were, because currently all she wanted was you. She wanted to use you to her heart's content until you were nothing but moans. She wanted to feast on you, because she was in dire hunger for your taste.

Coquelic taked her fingers across your pretty lingerie, before grabbing them and tearing them off your body. As much as she loved to see you, her little concubine dressed so prettily in something so revealing, it was blocking too much of your pretty skin. And she just couldn't have that.

"C-Coco!" You whined as she forced your legs wide apart after tossing your torn lingerie somewhere behind her.

"What?" Coquelic asked, looking up at you with a raised eyebrow, before noticing the pout on your face, and she glanced behind her at the heap of your lingerie on the ground, making her let out a laugh. "Don't fret, rosebud, I'll have one of the other concubines remake it for you."

"Okay..." You mumbled, still frowning. You had spent a great amount of time learning from Garofano on how to sew that, so it kinda hurt that Coquelic could so easily tear it apart without a care.

"Attention on me, rosebud." Coquelic demanded, nipping a bit roughly at your inner thigh, making you gasp and quickly look at her. "Good girl."

Your face flushes red as Coquelic begins to pepper your inner thighs in kisses, moving closer and closer to your dripping cunt. You whine for her, and with a small laugh, she moves her face to where you want her. She blows air on it first, watching it clench and drop more slick, making her lick her lips.

"My, such a pretty cunt..." Coquelic muses, sighing dreamily as she gazes at your hole. You flush red at her prolonged staring, subconsciously beginning to close your legs around her head.

Eyes flashing red, Coquelic's tail wraps tightly around one of your legs, forcefully pulling it back while she she pins your other with her hand, making sure you can't move them an inch. "Now now, don't be misbehaving."

"M'sorry!" You quickly apologize, moving to spread your legs wider for her (not that you could anymore) and lifting your hips slightly up as you presented your cunt for her.

"Good girl." Coquelic purred, softening her grip on you as she moves her head back in between your legs.

You let out a whine of pleasure as soon as Coquelic flicked her tongue across your dripping folds. She smirked and began to teasingly drag her tongue slowly past your lower lips, wiggling it about and making your walls clench around it tightly.

"Mmmm Coco..!" You whimpered at her teasing, pouting once again and staring down at her pleadingly, silently begging her to just give you her tongue.

Coquelic let out a breathy laugh, wanting to tease you further, but the ache of hunger in her stomach made her decide to give in. She shoved the rest of her tongue into your cunt, the pointed tip flicking against every inch it could get a taste of. The rest of her tongue wiggled around inside you, coating itself in your delicious juices.

She let out a pleased moan at the taste of you, her eyes rolling back in her head. She could feel her hunger ache more, needing your cum flooding her mouth. So, without much warning, she began to fuck her tongue in and out of your needy cunt.

You threw your head back, a silent scream leaving your mouth as you felt Coquelic's tongue thrusting in and out with such intensity you'd think it was her strap if you closed your eyes. Your hands flew down to her head, grabbing fistfuls of her hair into them.

You tugged her head closer, thighs trying to enclose around her head and keep her in place, but her tail tightened around your one leg again, keeping it firmly in place while her one hand did as well. You whined at the feeling but you didn't dare fight, instead letting your legs stay spread.

"Taste so good..." Coquelic moaned, the sound of her voice sending vibrations through you, making you jerk slightly, causing her to laugh.

She snaked her free hand up between your legs, settling it above your pussy. She pressed her thumb against your aching clit, putting pressure onto it and rubbing it slightly, immediately making you cry out of how good it felt. She rubbed it more as she continued her relentless pace of her tongue.

You could feel the coil in your gut beginning to tighten more and worsen, making tears spring to your eyes. It was becoming too much, and Coquelic knew that. But she was desperate for your taste so she only went faster and harder.

"C-Cumming!" You gasp out through moans before arching your back off the bed, shoving Coquelic's head farther between your thighs as you reach your peak.

Coquelic lets out a whine as you begin cumming, flooding her mouth. She held your spasming body down onto the bed, while diving her tongue deep into you, trying to drink up every last bit of your juices. She could feel relief flood her system as your taste soothes her aching hunger.

When she was satisfied, she slowly moved her head away from between your legs, licking her lips and chin that were drenched in your essence. You whimpered at the sight, and cowered under her lustful gaze. Her eyes were glowing more red, and you knew she wasn't completely satisfied just yet. She wasn't done using you for her own relief just yet.

ENDING NOTES: Anyone wane to get feasted on by Succubus!Coquelic?

[kurogane2512]

Not you all wanting to get railed by ptn mommies while theres me wanting to rail them-

(i want ro rail the top looking characters and make them beg for more as you tease them-)

And you are totally based for that anon because I personally prefer that too. I very much like getting teased by them and them initiating things but then I want to be the one to be inside them and rail them, even if I'm more sub oriented myself. I guess that makes me bit of a service top and I absolutely love making these doms as power bottoms.

Like imagine Chameleon hypnotizing you first and making you pleasure her but eventually she's so overestimulated that she breaks the spell and you get full control to do whatever and she gladly welcomes that because she needs it so much😏

Or Eirene who is so much into being serviced, she loves watching you below her and you worshipping her. She loves having you between her legs under her office table and eating her out but she absolutely lives for when you come out and bend her on that table to pound into her as you wish, oh the sweet moans and whispers she lets out😩

Then there's Cabernet who talks about how irresistible you are and how much she wants to pounce on you and eat you up. And she will- she will pin you down first and start with the most passionate kisses and licks, moaning how delicious you are but the moment you turn her over, she's gone. The way she'll clench your back and make scratches on your skin all the while being a moaning and whimpering mess in your embrace, oh yes she may love to dine but she also loves being dined on for the ultimate pleasure❤️

(I only wrote my favorite characters here but I very much relate with you anon, other characters I have similar thoughts on are Garofano, Chelsea, Langley and Coquelic)

[zwan99]

Welcome back grandma! (She never went anywhere in global server but still).

"Coquelic will also be receiving a 2* Attire, available permanently. It will be obtainable for free from the Supply Office.

As an apology for not commenting about the situation sooner, when the event reruns they will be giving out 10 pulls for free."

By Jiu Jiu subs

Free Coquelic Attire splash

[prisoner-of-sin]

ᴍᴀꜱᴛᴇʀʟɪꜱᴛ

○ - SFW

● - NSFW

Headcanons

○ Shy/Affectionate S/O [Zoya, Cinnabar, and Macchiato]

○ Kissing Headcanons [Langley]

Fics

● A Night of Passion [Langley] (18+)

● Wildfire [Zoya] (18+)

● Just Us [Adela] (18+)

Drabbles

○ Moments Alone [Coquelic]

Thirsts

● Breeding I [Zoya] (18+)

● Breeding II [Zoya] (18+)

○ Parenting [Zoya]

● Pregnant Sex [Zoya] (18+)

○ Parenting II [Zoya]

○ Parenting I [Rahu]

○ Types of Parents [Zoya, Rahu, Langley, Ninety-Nine, Shalom, Coquelic, and Cinnabar]

● Sex [Cinnabar]

● Sub Time [Rahu]

[stiri-noi]

Captură de 1,7 tone de răşină de canabis într-un camion condus de un român în tunelul de sub Canalul Mânecii - Adevărul

[wristwatchjournal]

Introducing – TRIBUS, a New British Watch Brand

A three-month manufacturing delay and a global pandemic haven’t deterred new luxury watch brand TRIBUS from launching with its line of timepieces inspired by dress watches from the 1940s and 50s. The company, founded by brothers Jake, Jonathan and James Ward, combines its Liverpudlian roots with Swiss watchmaking traditions. The company draws upon key childhood interests influenced greatly by the three siblings’ father, Christopher Ward, a passionate Liverpool FC supporter and a keen watch enthusiast, which explains the story behind its Premier League Champions watch. Also amongst the selection is a celebratory 80th-anniversary Battle of Britain wristwatch that pays tribute to a Polish squadron and its contribution towards defeating enemy aircraft during the Second World War. 

Each watch created by TRIBUS uses a Swiss-made case and a chronometer-certified movement manufactured in Lugano, Switzerland. The four models that comprise the brand’s core collection share what the company refers to as “retro-temporary” DNA. In addition to this dress watch styling, some models offer extra functions like power reserve indicators and GMT features. Having officially launched within the last week, TRIBUS is keen to share its heritage and how a combination of partnerships, along with its own series of timepieces, enables it to stand out. 

TRIBUS is Timing Partner of Liverpool Football Club

The TRI-08 Premier League Champions Special Edition line (priced between EUR 1,095 and 1,426) marks a multi-million-pound deal and a five-year partnership between TRIBUS and Liverpool Football Club. By taking advantage of the club’s huge global reach of 800 million followers, the timepieces bear LFC’s signature red colour on a three-handed dial and its crest embossed upon a recessed sub-counter at 6 o’clock. 

The 303 Squadron

Another special timepiece, the TRI-05 303 Squadron P8331 model is limited to 303 pieces and holds a metal disc from the arming panel of Piotr Laguna’s Spitfire P8331 RF-M “Sumatra”. The aircraft was shot down from the skies above Coquelles in 1941. Commemorating the Battle of Britain’s 80th anniversary, the 41mm steel model (priced at EUR 2,707) has a special engraved caseback and glass. Its black three-handed dial with luminous printed Arabic indices is equipped with black gunmetal hands propelled by a Sellita movement. Having collaborated with Laguna’s Spitfire Legacy (LSP) to create this limited edition watch, TRIBUS remembers the crucial role carried out by Polish servicemen who had their place at the British Victory March of 1946 wiped from their legacy.

The COSC-certified core range

The TRI-01 Small Second model (priced between EUR 1,426 and 1,536) is available in an unusual variety of dial colours. Aside from classic black and cream colours, iterations in salmon pink, yellow, and teal, fitted with either black or brown leather straps feature Arabic dials and a small second sub-dial at 6 o’clock. 

The TRI-02 GMT 3 Timezone model (priced between EUR 1,756 and 2,086) features a yellow GMT hand and an adjustable raised bezel marked out in a 24-hour scale. The frequent traveller can thus track three different timezones simultaneously. 

The TRI-03 Power Reserve GMT collection features a bi-compax dial available in cream or black. The power reserve sub-counter at 12 is balanced by a 24-hour sub-dial at 6 o’clock. The models available in steel or gold PVD range from EUR 2,086 to 2,197 respectively. 

Lastly, the 41mm TRI-04 Power Reserve GMT Sport, as its namesake suggests, is a more athletic-looking design. Red and yellow accented dials and black PVD-coated steel cases are offered on a choice of a bracelet, rubber strap or leather band.  

With a debut catalogue of designs that mix the old with the new, TRIBUS is a watch brand that focusses on collaborating with some left-field partnerships to align its British heritage with reliable Swiss precision.

More details about the brand and its models here, at tribus-watches.com.

The post Introducing – TRIBUS, a New British Watch Brand appeared first on Wristwatch Journal.

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[abbkineeu]

New Post has been published on Biotech Advisers

New Post has been published on http://www.bioadvisers.com/renewing-felsensteins-phylogenetic-bootstrap-era-big-data/

Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Content introduction：

A randomized trial of normothermic preservation in liver transplantation

SAMHD1 acts at stalled replication forks to prevent interferon induction

Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Laser spectroscopic characterization of the nuclear-clock isomer 229mTh

Identification of the tumour transition states occurring during EMT

1. A randomized trial of normothermic preservation in liver transplantation

Liver transplantation is a highly successful treatment, but is severely limited by the shortage in donor organs. However, many potential donor organs cannot be used; this is because sub-optimal livers do not tolerate conventional cold storage and there is no reliable way to assess organ viability preoperatively. Normothermic machine perfusion maintains the liver in a physiological state, avoids cooling and allows recovery and functional testing. Here David Nasralla at University of Oxford in Oxford, UK and his colleagues show that, in a randomized trial with 220 liver transplantations, compared to conventional static cold storage, normothermic preservation is associated with a 50% lower level of graft injury, measured by hepatocellular enzyme release, despite a 50% lower rate of organ discard and a 54% longer mean preservation time. There was no significant difference in bile duct complications, graft survival or survival of the patient. If translated to clinical practice, these results would have a major impact on liver transplant outcomes and waiting list mortality.

Read more, please click https://www.nature.com/articles/s41586-018-0047-9

2. SAMHD1 acts at stalled replication forks to prevent interferon induction

SAMHD1 was previously characterized as a dNTPase that protects cells from viral infections. Mutations in SAMHD1 are implicated in cancer development and in a severe congenital inflammatory disease known as Aicardi–Goutières syndrome. The mechanism by which SAMHD1 protects against cancer and chronic inflammation is unknown. Here Flavie Coquel at University of Montpellier in Montpellier, France and his colleagues show that SAMHD1 promotes degradation of nascent DNA at stalled replication forks in human cell lines by stimulating the exonuclease activity of MRE11. This function activates the ATR–CHK1 checkpoint and allows the forks to restart replication. In SAMHD1-depleted cells, single-stranded DNA fragments are released from stalled forks and accumulate in the cytosol, where they activate the cGAS–STING pathway to induce expression of pro-inflammatory type I interferons. SAMHD1 is thus an important player in the replication stress response, which prevents chronic inflammation by limiting the release of single-stranded DNA from stalled replication forks.

Read more, please click https://www.nature.com/articles/s41586-018-0050-1

3. Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Felsenstein’s application of the bootstrap method to evolutionary trees is one of the most cited scientific papers of all time. The bootstrap method, which is based on resampling and replications, is used extensively to assess the robustness of phylogenetic inferences. However, increasing numbers of sequences are now available for a wide variety of species, and phylogenies based on hundreds or thousands of taxa are becoming routine. With phylogenies of this size Felsenstein’s bootstrap tends to yield very low supports, especially on deep branches. Here F. Lemoine at Institut Pasteur & CNRS in Paris, France and his colleagues propose a new version of the phylogenetic bootstrap in which the presence of inferred branches in replications is measured using a gradual ‘transfer’ distance rather than the binary presence or absence index used in Felsenstein’s original version. The resulting supports are higher and do not induce falsely supported branches. The application of their method to large mammal, HIV and simulated datasets reveals their phylogenetic signals, whereas Felsenstein’s bootstrap fails to do so.

Read more, please click https://www.nature.com/articles/s41586-018-0043-0

4. Laser spectroscopic characterization of the nuclear-clock isomer 229mTh

The isotope 229Th is the only nucleus known to possess an excited state 229mTh in the energy range of a few electronvolts—a transition energy typical for electrons in the valence shell of atoms, but about four orders of magnitude lower than typical nuclear excitation energies. Of the many applications that have been proposed for this nuclear system, which is accessible by optical methods, the most promising is a highly precise nuclear clock that outperforms existing atomic timekeepers. Here Johannes Thielking at Physikalisch-Technische Bundesanstalt in Braunschweig, Germany and his colleagues present the laser spectroscopic investigation of the hyperfine structure of the doubly charged 229mTh ion and the determination of the fundamental nuclear properties of the isomer, namely, its magnetic dipole and electric quadrupole moments, as well as its nuclear charge radius. Following the recent direct detection of this long-sought isomer, they provide detailed insight into its nuclear structure and present a method for its non-destructive optical detection.

Read more, please click https://www.nature.com/articles/s41586-018-0011-8

5. Identification of the tumour transition states occurring during EMT

In cancer, the epithelial-to-mesenchymal transition (EMT) is associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However, there is no direct in vivo evidence for this idea. Here Ievgenia Pastushenko at Université Libre de Buxelles in Brussels, Belgium and his colleagues screen a large panel of cell surface markers in skin and mammary primary tumours, and identify the existence of multiple tumour subpopulations associated with different EMT stages: from epithelial to completely mesenchymal states, passing through intermediate hybrid states. Although all EMT subpopulations presented similar tumour-propagating cell capacity, they displayed differences in cellular plasticity, invasiveness and metastatic potential. Their transcriptional and epigenetic landscapes identify the underlying gene regulatory networks, transcription factors and signalling pathways that control these different EMT transition states. Finally, these tumour subpopulations are localized in different niches that differentially regulate EMT transition states.

Read more, please click https://www.nature.com/articles/s41586-018-0040-3

[bioadvisers]

Bioadvisers shared on Biotech Advisers

Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Content introduction：

A randomized trial of normothermic preservation in liver transplantation

SAMHD1 acts at stalled replication forks to prevent interferon induction

Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Laser spectroscopic characterization of the nuclear-clock isomer 229mTh

Identification of the tumour transition states occurring during EMT

1. A randomized trial of normothermic preservation in liver transplantation

Liver transplantation is a highly successful treatment, but is severely limited by the shortage in donor organs. However, many potential donor organs cannot be used; this is because sub-optimal livers do not tolerate conventional cold storage and there is no reliable way to assess organ viability preoperatively. Normothermic machine perfusion maintains the liver in a physiological state, avoids cooling and allows recovery and functional testing. Here David Nasralla at University of Oxford in Oxford, UK and his colleagues show that, in a randomized trial with 220 liver transplantations, compared to conventional static cold storage, normothermic preservation is associated with a 50% lower level of graft injury, measured by hepatocellular enzyme release, despite a 50% lower rate of organ discard and a 54% longer mean preservation time. There was no significant difference in bile duct complications, graft survival or survival of the patient. If translated to clinical practice, these results would have a major impact on liver transplant outcomes and waiting list mortality.

Read more, please click https://www.nature.com/articles/s41586-018-0047-9

2. SAMHD1 acts at stalled replication forks to prevent interferon induction

SAMHD1 was previously characterized as a dNTPase that protects cells from viral infections. Mutations in SAMHD1 are implicated in cancer development and in a severe congenital inflammatory disease known as Aicardi–Goutières syndrome. The mechanism by which SAMHD1 protects against cancer and chronic inflammation is unknown. Here Flavie Coquel at University of Montpellier in Montpellier, France and his colleagues show that SAMHD1 promotes degradation of nascent DNA at stalled replication forks in human cell lines by stimulating the exonuclease activity of MRE11. This function activates the ATR–CHK1 checkpoint and allows the forks to restart replication. In SAMHD1-depleted cells, single-stranded DNA fragments are released from stalled forks and accumulate in the cytosol, where they activate the cGAS–STING pathway to induce expression of pro-inflammatory type I interferons. SAMHD1 is thus an important player in the replication stress response, which prevents chronic inflammation by limiting the release of single-stranded DNA from stalled replication forks.

Read more, please click https://www.nature.com/articles/s41586-018-0050-1

3. Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Felsenstein’s application of the bootstrap method to evolutionary trees is one of the most cited scientific papers of all time. The bootstrap method, which is based on resampling and replications, is used extensively to assess the robustness of phylogenetic inferences. However, increasing numbers of sequences are now available for a wide variety of species, and phylogenies based on hundreds or thousands of taxa are becoming routine. With phylogenies of this size Felsenstein’s bootstrap tends to yield very low supports, especially on deep branches. Here F. Lemoine at Institut Pasteur & CNRS in Paris, France and his colleagues propose a new version of the phylogenetic bootstrap in which the presence of inferred branches in replications is measured using a gradual ‘transfer’ distance rather than the binary presence or absence index used in Felsenstein’s original version. The resulting supports are higher and do not induce falsely supported branches. The application of their method to large mammal, HIV and simulated datasets reveals their phylogenetic signals, whereas Felsenstein’s bootstrap fails to do so.

Read more, please click https://www.nature.com/articles/s41586-018-0043-0

4. Laser spectroscopic characterization of the nuclear-clock isomer 229mTh

The isotope 229Th is the only nucleus known to possess an excited state 229mTh in the energy range of a few electronvolts—a transition energy typical for electrons in the valence shell of atoms, but about four orders of magnitude lower than typical nuclear excitation energies. Of the many applications that have been proposed for this nuclear system, which is accessible by optical methods, the most promising is a highly precise nuclear clock that outperforms existing atomic timekeepers. Here Johannes Thielking at Physikalisch-Technische Bundesanstalt in Braunschweig, Germany and his colleagues present the laser spectroscopic investigation of the hyperfine structure of the doubly charged 229mTh ion and the determination of the fundamental nuclear properties of the isomer, namely, its magnetic dipole and electric quadrupole moments, as well as its nuclear charge radius. Following the recent direct detection of this long-sought isomer, they provide detailed insight into its nuclear structure and present a method for its non-destructive optical detection.

Read more, please click https://www.nature.com/articles/s41586-018-0011-8

5. Identification of the tumour transition states occurring during EMT

In cancer, the epithelial-to-mesenchymal transition (EMT) is associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However, there is no direct in vivo evidence for this idea. Here Ievgenia Pastushenko at Université Libre de Buxelles in Brussels, Belgium and his colleagues screen a large panel of cell surface markers in skin and mammary primary tumours, and identify the existence of multiple tumour subpopulations associated with different EMT stages: from epithelial to completely mesenchymal states, passing through intermediate hybrid states. Although all EMT subpopulations presented similar tumour-propagating cell capacity, they displayed differences in cellular plasticity, invasiveness and metastatic potential. Their transcriptional and epigenetic landscapes identify the underlying gene regulatory networks, transcription factors and signalling pathways that control these different EMT transition states. Finally, these tumour subpopulations are localized in different niches that differentially regulate EMT transition states.

Read more, please click https://www.nature.com/articles/s41586-018-0040-3

[abbkine]

BioAdvisers said on Biotech Advisers

Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Content introduction：

A randomized trial of normothermic preservation in liver transplantation

SAMHD1 acts at stalled replication forks to prevent interferon induction

Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Laser spectroscopic characterization of the nuclear-clock isomer 229mTh

Identification of the tumour transition states occurring during EMT

1. A randomized trial of normothermic preservation in liver transplantation

Liver transplantation is a highly successful treatment, but is severely limited by the shortage in donor organs. However, many potential donor organs cannot be used; this is because sub-optimal livers do not tolerate conventional cold storage and there is no reliable way to assess organ viability preoperatively. Normothermic machine perfusion maintains the liver in a physiological state, avoids cooling and allows recovery and functional testing. Here David Nasralla at University of Oxford in Oxford, UK and his colleagues show that, in a randomized trial with 220 liver transplantations, compared to conventional static cold storage, normothermic preservation is associated with a 50% lower level of graft injury, measured by hepatocellular enzyme release, despite a 50% lower rate of organ discard and a 54% longer mean preservation time. There was no significant difference in bile duct complications, graft survival or survival of the patient. If translated to clinical practice, these results would have a major impact on liver transplant outcomes and waiting list mortality.

Read more, please click https://www.nature.com/articles/s41586-018-0047-9

2. SAMHD1 acts at stalled replication forks to prevent interferon induction

SAMHD1 was previously characterized as a dNTPase that protects cells from viral infections. Mutations in SAMHD1 are implicated in cancer development and in a severe congenital inflammatory disease known as Aicardi–Goutières syndrome. The mechanism by which SAMHD1 protects against cancer and chronic inflammation is unknown. Here Flavie Coquel at University of Montpellier in Montpellier, France and his colleagues show that SAMHD1 promotes degradation of nascent DNA at stalled replication forks in human cell lines by stimulating the exonuclease activity of MRE11. This function activates the ATR–CHK1 checkpoint and allows the forks to restart replication. In SAMHD1-depleted cells, single-stranded DNA fragments are released from stalled forks and accumulate in the cytosol, where they activate the cGAS–STING pathway to induce expression of pro-inflammatory type I interferons. SAMHD1 is thus an important player in the replication stress response, which prevents chronic inflammation by limiting the release of single-stranded DNA from stalled replication forks.

Read more, please click https://www.nature.com/articles/s41586-018-0050-1

3. Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Felsenstein’s application of the bootstrap method to evolutionary trees is one of the most cited scientific papers of all time. The bootstrap method, which is based on resampling and replications, is used extensively to assess the robustness of phylogenetic inferences. However, increasing numbers of sequences are now available for a wide variety of species, and phylogenies based on hundreds or thousands of taxa are becoming routine. With phylogenies of this size Felsenstein’s bootstrap tends to yield very low supports, especially on deep branches. Here F. Lemoine at Institut Pasteur & CNRS in Paris, France and his colleagues propose a new version of the phylogenetic bootstrap in which the presence of inferred branches in replications is measured using a gradual ‘transfer’ distance rather than the binary presence or absence index used in Felsenstein’s original version. The resulting supports are higher and do not induce falsely supported branches. The application of their method to large mammal, HIV and simulated datasets reveals their phylogenetic signals, whereas Felsenstein’s bootstrap fails to do so.

Read more, please click https://www.nature.com/articles/s41586-018-0043-0

4. Laser spectroscopic characterization of the nuclear-clock isomer 229mTh

The isotope 229Th is the only nucleus known to possess an excited state 229mTh in the energy range of a few electronvolts—a transition energy typical for electrons in the valence shell of atoms, but about four orders of magnitude lower than typical nuclear excitation energies. Of the many applications that have been proposed for this nuclear system, which is accessible by optical methods, the most promising is a highly precise nuclear clock that outperforms existing atomic timekeepers. Here Johannes Thielking at Physikalisch-Technische Bundesanstalt in Braunschweig, Germany and his colleagues present the laser spectroscopic investigation of the hyperfine structure of the doubly charged 229mTh ion and the determination of the fundamental nuclear properties of the isomer, namely, its magnetic dipole and electric quadrupole moments, as well as its nuclear charge radius. Following the recent direct detection of this long-sought isomer, they provide detailed insight into its nuclear structure and present a method for its non-destructive optical detection.

Read more, please click https://www.nature.com/articles/s41586-018-0011-8

5. Identification of the tumour transition states occurring during EMT

In cancer, the epithelial-to-mesenchymal transition (EMT) is associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However, there is no direct in vivo evidence for this idea. Here Ievgenia Pastushenko at Université Libre de Buxelles in Brussels, Belgium and his colleagues screen a large panel of cell surface markers in skin and mammary primary tumours, and identify the existence of multiple tumour subpopulations associated with different EMT stages: from epithelial to completely mesenchymal states, passing through intermediate hybrid states. Although all EMT subpopulations presented similar tumour-propagating cell capacity, they displayed differences in cellular plasticity, invasiveness and metastatic potential. Their transcriptional and epigenetic landscapes identify the underlying gene regulatory networks, transcription factors and signalling pathways that control these different EMT transition states. Finally, these tumour subpopulations are localized in different niches that differentially regulate EMT transition states.

Read more, please click https://www.nature.com/articles/s41586-018-0040-3

[sporadic-gremlin]

what if size kink with coquelic

implied power bottom Coquelic

she loves the difference and she makes it her goal to show you she loves it too. she’ll ride you, caressing the bulge you’re making and smile down at you all while rolling her hips. she’ll force you to keep your eyes on her and if they even waver for a second, she’ll stop moving because she knows that you can’t keep your eyes off of the way her pussy wraps so perfectly around your cock.

in my personal hc, coquelic has a mirror in her room or the chiefs room which she only uses to watch your cock sink into her. something of that size should not be able to fit inside her but it does and feels so fucking good. god, she doesn’t even realize her lip is bleeding until you lick or wipe it off. and her fangs—when you’re rutting into her, every inch of you stretching her out, expect some pretty deep bite marks.

and she cries or at least gets teary eyed when the pleasure comes a little too close to being overwhelming. coquelic will let out these pretty little gasps and whines, dragging her nails down your back or tangling them in your hair but it only fuels you to fuck her harder. and when she cums, her pussy clenches so tight it damn near pushes you out.

and at the end of it, you love watching her gaping pussy clench around absolutely nothing as your cum leaks out of it

[zwan99]

CN News: Path to Nowhere Devs' Future plans

All translated by Jiu Jiu subs (@ qwertdwlrma on Twitter):

1) Coquelic is returning

2) BFL DZ optimization

3) Surveillance Order Optimization

4) Preparation of New Content

1) Coquelic

They are finally able to say with certainty that Coquelic will be returning. They are already working on adjusting Coquelic's base art. Coquelic will have a rate-up banner in July. Rain Burst + Floral Unfurl will rerun at that same time.

After mid-June, Coquelic will rejoin the standard, routine, event, etc off-rate pool for CN.

In Global she was never removed.

Coquelic will also be receiving a 2* Attire, available for a limited time. It will be obtainable for free from the Supply Office.

As an apology for not commenting about the situation sooner, when the event reruns they will be giving out 10 pulls for free.

2) BFL

- A recommended lineup feature and increased Sunday attempts, so you can reference who to use and also more freely choose when you want to play.

When designing BFL, they wanted a place where Chiefs could prove their teams' strength and see the progress of their Sinners. However, long-term, the cyclical nature and high difficulty of it created too much pressure for players. In June, they will be changing BFL:

- Implement points and attempts retention within each season. If you beat a boss and earn enough points, you will be able to claim the rewards next week without needing to rechallenge it.

- New mode where you can get rewards for 160k (single boss)/500k (all three total) by simply spending attempts rather than it being based on score.

3) Surveillance Order

They received feedback that the SO cap was too high and could not be reached. They plan to make the following improvements in July when the next new SO releases:

- Players that purchase Advanced/In-Depth will receive additional Surveillance Emblems

- Lower the upper limit level of SO to lv 100. After this adjustment, players that purchase Advanced SO will be able to fully complete it.

4) New Content

In the recent questionnaire, they received a lot of feedback expectant about new content. Rest assured that the production team has been working and planning on that.

A completely new limited gamemode will be launching in August, and will be futher optimised based on peoples' feedback.

They are also working on new main story content.

[zwan99]

Advice for upcoming banners from someone who actually knows what they're talking about (certainly not me):

"Pull advice IF you care about meta:

- Skip Rahu

- Pull Shalom [S0 or S3]

- Pull Etti if lack Magic burst [S1]

- Pull Mantis if no Deren and lack Physical burst [S1]

- Skip Collective

- Skip Eleven

- Pull Dreya (Reruns after Eleven) [S1]

Coquelic is strong in long battles, BUT requires high investment.

She requires at least S1 to be useable, and ideally wants S3/S4. You make an Atkspd comp with her by pairing her with fast Normal Attackers (i.e. Summer, Pricilla)."

If you have questions go ask them on twitter: Jiu Jiu subs (@ qwertdwlrma)

All credits to Jiu Jiu subs

[bioadvisers]

Bioadvisers shared on Biotech Advisers

Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Content introduction：

A randomized trial of normothermic preservation in liver transplantation

SAMHD1 acts at stalled replication forks to prevent interferon induction

Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Laser spectroscopic characterization of the nuclear-clock isomer 229mTh

Identification of the tumour transition states occurring during EMT

1. A randomized trial of normothermic preservation in liver transplantation

Liver transplantation is a highly successful treatment, but is severely limited by the shortage in donor organs. However, many potential donor organs cannot be used; this is because sub-optimal livers do not tolerate conventional cold storage and there is no reliable way to assess organ viability preoperatively. Normothermic machine perfusion maintains the liver in a physiological state, avoids cooling and allows recovery and functional testing. Here David Nasralla at University of Oxford in Oxford, UK and his colleagues show that, in a randomized trial with 220 liver transplantations, compared to conventional static cold storage, normothermic preservation is associated with a 50% lower level of graft injury, measured by hepatocellular enzyme release, despite a 50% lower rate of organ discard and a 54% longer mean preservation time. There was no significant difference in bile duct complications, graft survival or survival of the patient. If translated to clinical practice, these results would have a major impact on liver transplant outcomes and waiting list mortality.

Read more, please click https://www.nature.com/articles/s41586-018-0047-9

2. SAMHD1 acts at stalled replication forks to prevent interferon induction

SAMHD1 was previously characterized as a dNTPase that protects cells from viral infections. Mutations in SAMHD1 are implicated in cancer development and in a severe congenital inflammatory disease known as Aicardi–Goutières syndrome. The mechanism by which SAMHD1 protects against cancer and chronic inflammation is unknown. Here Flavie Coquel at University of Montpellier in Montpellier, France and his colleagues show that SAMHD1 promotes degradation of nascent DNA at stalled replication forks in human cell lines by stimulating the exonuclease activity of MRE11. This function activates the ATR–CHK1 checkpoint and allows the forks to restart replication. In SAMHD1-depleted cells, single-stranded DNA fragments are released from stalled forks and accumulate in the cytosol, where they activate the cGAS–STING pathway to induce expression of pro-inflammatory type I interferons. SAMHD1 is thus an important player in the replication stress response, which prevents chronic inflammation by limiting the release of single-stranded DNA from stalled replication forks.

Read more, please click https://www.nature.com/articles/s41586-018-0050-1

3. Renewing Felsenstein’s phylogenetic bootstrap in the era of big data

Felsenstein’s application of the bootstrap method to evolutionary trees is one of the most cited scientific papers of all time. The bootstrap method, which is based on resampling and replications, is used extensively to assess the robustness of phylogenetic inferences. However, increasing numbers of sequences are now available for a wide variety of species, and phylogenies based on hundreds or thousands of taxa are becoming routine. With phylogenies of this size Felsenstein’s bootstrap tends to yield very low supports, especially on deep branches. Here F. Lemoine at Institut Pasteur & CNRS in Paris, France and his colleagues propose a new version of the phylogenetic bootstrap in which the presence of inferred branches in replications is measured using a gradual ‘transfer’ distance rather than the binary presence or absence index used in Felsenstein’s original version. The resulting supports are higher and do not induce falsely supported branches. The application of their method to large mammal, HIV and simulated datasets reveals their phylogenetic signals, whereas Felsenstein’s bootstrap fails to do so.

Read more, please click https://www.nature.com/articles/s41586-018-0043-0

4. Laser spectroscopic characterization of the nuclear-clock isomer 229mTh

The isotope 229Th is the only nucleus known to possess an excited state 229mTh in the energy range of a few electronvolts—a transition energy typical for electrons in the valence shell of atoms, but about four orders of magnitude lower than typical nuclear excitation energies. Of the many applications that have been proposed for this nuclear system, which is accessible by optical methods, the most promising is a highly precise nuclear clock that outperforms existing atomic timekeepers. Here Johannes Thielking at Physikalisch-Technische Bundesanstalt in Braunschweig, Germany and his colleagues present the laser spectroscopic investigation of the hyperfine structure of the doubly charged 229mTh ion and the determination of the fundamental nuclear properties of the isomer, namely, its magnetic dipole and electric quadrupole moments, as well as its nuclear charge radius. Following the recent direct detection of this long-sought isomer, they provide detailed insight into its nuclear structure and present a method for its non-destructive optical detection.

Read more, please click https://www.nature.com/articles/s41586-018-0011-8

5. Identification of the tumour transition states occurring during EMT

In cancer, the epithelial-to-mesenchymal transition (EMT) is associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However, there is no direct in vivo evidence for this idea. Here Ievgenia Pastushenko at Université Libre de Buxelles in Brussels, Belgium and his colleagues screen a large panel of cell surface markers in skin and mammary primary tumours, and identify the existence of multiple tumour subpopulations associated with different EMT stages: from epithelial to completely mesenchymal states, passing through intermediate hybrid states. Although all EMT subpopulations presented similar tumour-propagating cell capacity, they displayed differences in cellular plasticity, invasiveness and metastatic potential. Their transcriptional and epigenetic landscapes identify the underlying gene regulatory networks, transcription factors and signalling pathways that control these different EMT transition states. Finally, these tumour subpopulations are localized in different niches that differentially regulate EMT transition states.

Read more, please click https://www.nature.com/articles/s41586-018-0040-3

[vrachis]

???? HOLY SHIT???? 🔥

oh my fod this was so hot. im. hard af

seems like fano wants a turn too 🤭

Feeling quite moody today after having exams… all I want to do is to fuck and break coquelic, absolutely destroy her till she’s babbling nonsense and wanting more. And doing it in front of the other Garden members? Fuck yeah.

"god, you feel so—" your words are cut off with a moan, head involuntarily tilting forward as coquelic's pussy clenches around your cock.

coquelic arches off the bed, a whimper squeezing its way past her lips. her skin was flushed and damp but right now, she was nothing if not the image of divinity. those iridescent ruby eyes snapped shut as another orgasm tore through her, tears beading in the corners of her eyes. even as she came, you fucked her through it, folding her into a mating press.

"yes, right there!..." coquelic choked. you saw the way her chested heaved as she tried her best to catch her breath before you fucked it back out of her.

"fuck, 'm gonna cum," you groan, gritting your teeth as waves of euphoria took a hold of you, cock throbbing as ropes of warm cum filled coqeulic’s pussy. “do you think she deserves another, garofano?”

garofano hummed and narrowed her eyes at her mentor. coquelic has always been gorgeous but there’s just something about seeing her in this state that really captured her essence… “i think she does.”

you have a dopey smile to garofano before pulling coquelic’s legs over your shoulders, pressing sloppy kisses to the inside of her thigh. fuck, you were so sensitive but coquelic looked so pretty like this. it felt like coquelic was trying to push you out with the way she was pulsing around you but as you rolled your hips, your cock made its home deep inside her warmth.

“ohgodfuck, ‘m so close, ‘m gonna c—fuck!—cum,” coquelic sobbed, gripping at garofano’s dress. coquelic gasped and panted, trying to catch her breath as her orgasm ripped it from her.

you moaned, thrusting into her a little bit harder than before. “that’s it, baby. fuck, you feel so good around me.”

garofano blushed a little at your words. you were both so lost in each other that you must’ve forgotten that there were others in the room with you…