#stefee

[harringroveera]

Hopper is strict on all of his kids’s boyfriends actually

[ronanceisintheair]

s3 starts with too many jumpscares: the make-up they did for scoops Steve. The Mike and El kissing scene.

Like I'm not your strongest soldier I promise, please.

[thelastdj]

they killed my babygirl 😔

[inspiredwriter]

*Una noche en la pizzería Fazbaer tortuga*

Stefany 2018, Anastasia 2018, April 2018 and Candy 2018 :*entra la oficina de seguridad*

Stefany 2018 :* se acomda la gorra*mis chicas hoy será nuestra gran noche de trabajo en la pizzería Fazbaer tortuga 😉😁🏪🍕✨

April 2018 : estoy de acuerdo Stefes, increíble que William nos contrató como guardia de seguridad para cuidar el restaurante genial de todos los tiempos con lo más asombroso animatrónicos😄😊👍👮‍♀️🔦🤖*aprieta sus puños*o si viene ladrón y robar cosas o el dinero le daremos un merecido😈😁💰✊💥

Anastasia 2018 :*mirá las cámaras y ve las tortugas*wow, vamos a cuidar los animatrónicos más geniales del mundo🤩☺️🤖🐢��*señala la imagen de Mikey*pero Mikey es mi favorita como la pizza 🥰😋🍕❣️💗💞

Candy 2018 :*se cruza los brazos* eso ya lo sabemos Anastasia tranquilízate🙄😏📹*mira las cámaras*pero tenemos que tener cuidado escuché que la pizzería está embrujada que se fueron los antiguos trabajadores 🤔😟👮‍♂️👻🏪

Stefany 2018 : no existen los fantasmas Candy 🤨😒🚫👻*toma las papeles y los revisa* Acabo de revisar el papeleo qué los trabajadores renunciaron por miedo de los animatrónicos que cobran vida en la noche 🤔😟🤖🌃📄

Anastasia 2018 : Oh si Yo también revisé el papel que eran solo los antiguos trabajadores que trabajaban 😕🙁📑*muestra la foto las chicas*pero dijeron que se habían desaparecido por un accidente de un oso dorado 😣😨💦🐻🎩 no quiero que me lastime o peor nos meterá en trajes de animatrónicos😱😰🤖💦

April 2018 :*abraza a Anastasia* No te preocupes hermana no tienes que asustarte estoy aquí para protegerte 😉🤗💗💞❣️

Anastasia 2018 : tiene razón tú siempre me proteges de los miedos del peligro 😃😄💕💓✨

Stefany 2018 : pienso lo correcto no estás ya todos estaremos a salvo en la oficina solo hay que cerrar las puertas revisar las cámaras 😊😏🚪🔐📹*toma la tableta y no ve los Animatrónicos* Oh dios los animatrónicos están estaban a aquí un segundo😲😰💦🐻🐰🐥

Candy 2018 :*mirá cámara y ve la imagen de Raph* solo veo a Raph el Cove Kids por fuera de servicio 😟🤔🏴‍☠️ Pero qué pasa si están vivos y escondidos para asustarnos 😣😨👻

Stefany 2018 : Supongo que tenemos que revisar todo el lugar de la pizzería🤨😔🔦🏪*saca las linternas del cajón* Anastasia, quiero que revises en la cocina 🍳🍕April, tú revisarás en el cuarto de servicio 🚪⚙️🧰Candy, tú entrarás al Cove Kids y no se llevaron a Rafa El pirata 🏴‍☠️🔦yo, me quedaré Aquí revisando las cámaras de seguridad😤😉📹🖥️

Anastasia 2018, April 2018 and Candy 2018 : como gustes capitana 🫡🫡🫡*salen de la oficina*

Leo 2018 :*mirá a Stefany en escondidas de la ventana* jajaja~😄😈

@inspiredwriter

Stefany 2018: Hmm, was it just me or did someone laugh just now?🤨 *Examines CCTV cameras* I can't imagine where the animatronics could have gone, they are not in the kitchen or in the playroom..🤔😕🍽🎁🎈 Wait, I see something in the corridor...😥 Oh God, Leonardo is very close, I have to lock the door immediately!😱😫🚪🔐 *Locks the door*

Leo 2018: *Knocks on the window* (Thoughts💭) Shell, the security guard noticed me before I could burst into the office!✊😡👿

Stefany 2018: Oh, how scary his eyes shine in the dark...😰💦👀 *Turns on the flashlight and points it at Leo* Maybe if I shine the flashlight in his face, he will go away, because I have very little energy and soon the door will open😥😖🔦🚪🪫

Leo 2018: *Closes eyes and bends over* Ouch!😩😖 I almost burned my eyes with this stupid light...🤬😤🔦 But I would like to get a better look at what the new security guard looks like🤔🤨 *Covering his eyes with a hand, looks at Stefany* ...Oh my pizza slices~!😳😲🍕💘💖❣️💕

Stefany 2018: *Blushes* Huh?😳😧 Why is he looking at me so strangely?..😳💓💘🩷💞

*In the kitchen*

Anastasia 2018: *Walks through the kitchen, humming the song "Hello, my baby" by Chordettes* Jeez, when I hum it, I don't feel so scared😅😊🎶 But I have to be careful, because the animatronics may be somewhere nearby😰😥🔦

Mikey 2018: *Hiding in the dark* (Thoughts💭) Hahaha, when the right moment comes, I will suddenly jump out and scare the security guard😈😆 ...But Leo didn't warn me that the new security guard is a girl😧😳👮‍♀️📹 I see with my night vision eyes that she is very beautiful and singing so pretty~😀😍💖💝💗💕

*In the utility room*

April 2018: *Walks through the room* Oh, it's so cold and gloomy here...😰 I don't think going looking for the animatronics alone was a good idea, I hope none of them attacked my little sister. I'm so worried about her...😥😖 Ah, it seems like there's something iron behind me😧😰 *Shines a flashlight and sees Donnie* Aaah?!😱🔦

Donnie 2018: It was very nice of you to snuggle up to my metal chest frame😉😈 (Thoughts💭) Wow, I must admit, this girl looks very beautiful for security guard~😳🥰👮‍♀️💓❤💘💞 Now I don’t even know how I can grab her and put her in the animatronic costume...🤔🥺🤖💗💓💕

*In the playroom*

Candy 2018: *Opens the curtain of the pirate cove and shines a flashlight inside* It looks like Raphael really left his pirate cove😕🔦🏴‍☠️ I can't believe that such scary machines were created to entertain children...😓🎉🎂🎊🧒👦👧 Brrr, I'm a grown girl and the thought of meeting one of them who wants to scare me, makes my skin crawl...😖😥

Raph 2018: *Sneaks up behind Candy* (Thoughts) Mmmh, hahaha, get ready to attack the ship, pirates...😈⛵🏴‍☠️ *Looks at Candy's back and booty* Wait a second, my only eye spotted a gorgeous mermaid on the horizon!~😃😍🧜‍♀️💝💘❤💕 Such a beauty like she is dangerous to stay here, so I have to quickly lead her to the exit...🥺🥰💗💓💝💞 But her work clothes..🤨 She is the security guard?!😲😧👮‍♀️📹

@swagreecrow

[mahekinteractive]

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[birthday-bash14]

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[444names]

Names generated from hypocorisms, theophoric and Irish names, excluding the letter "H"

Abbilín Abdie Abdubay Abraelín Afranie Ainníbay Airie Aisiano Ambre Ameandie Ament Ammis Amonnéill Amormos Anuatty Anuel Aoindy Aoisreas Aolie Aomás Aormann Apold Arana Arisenán Arnistín Arreggy Arrie...

Bairi Baoill Baolly Baram Barbrinn Barnín Beace Beallait Beammary Beank Bearie Bearlia Bearnán Bekev Bekevice Beknailly Biblas Bigussie Biline Blaid Bogdavy Bogen Bogobnace Bogoil Brall Brear Brearí Brete Briamuric Brisenn Broisi Bréan Buaiggie Buatty Bufed Bufeitzi Caignemmy Cainn Caitlís Cally Cammilín Caoibby Caoill Caoilín Caoimeret Caoisíony Caolm Carcy Carni Carnán Catiodonn Catun Ciait Ciano Cianre Ciarcearn Ciarcy Ciargabie Cinew Cinny Ciodori Cioncel Clain Clemsany Clill Clinel Coben Cobóis Coibe Coiblán Coinny Coird Coluck Comáinnie Conal Conaomi Conas Connán Corliffy Cormaitz Corna Corry Críde Crídes Cuinsi Daigi Dainnus Dameabie Danatie Dandy Danouimmy Darnán Datra Davdony Davee Daverny Davic Davić Dawinn Dearka Dearrd Declie Dedie Dedun Deenuel Della Denmoswal Dericky Deruas Deudian Diadote Diame Diamen Diarj Diggy Dolán Donnia Dorean Dorla Dorongie Dorty Déaggie Déaolle Dúnlu Eagail Earny Edeimenie Eggarry Eimín Eirgus Eirie Eirín Eitéardie Eleárla Elsiarce Emeimídín Eminm Emmine Errias Fearigney Fearnín Feinise Feirrewt Ferie Ferníle Fiarne Fince Fioben Fionné Fiony Flais Flock Floddy Frenán Féigginn Fíonnc Fíosna Gabraindy Gailly Garristín Garry Gerdie Gerne Gilizzie Gláit Goviall Grait Greuel Gréammy Gusepe Iarny Iasarrd Iasán Ionnán Ionnín Iregy Irekenjy Irnán Isamadna Isikennad Isliff Issias Istannán Izzie Ióses Iúdáile Iúdán Jeddy Jedeudi Jenji Jernán Jimmikie Joele Jonán Jooddy Josebe Joset Josnaiss Judavick Kammy Karlart Kateff Kiell Koláinm Kolán Kwagán Laireann Lanial Lanicke Lanie Lanniamen Larmaolm Lasiggar Lasmenty Laure Leseb Libasnac Limentóin Lionait Lisea Lizzy Locky Lodougić Lomás Lonall Lugie Luird Luire Magán Maibel Mainn Mairearis Mamenie Mamer Mandán Mankie Mannceas Mannya Manorent Maoda Maodi Maodo Maodou Maoillad Maoin Maoir Maoire Maoll Maolli Maolmán Maose Marry Marta Menef Mennick Merus Milby Milie Milíony Miomórl Monie Morry Moseán Moslás Muiel Muinny Muire Muirear Muirí Muirín Muncear Máinais Mídeibal Mídín Móinc Mórle Naidy Nairicke Naoll Niara Nidouim Nolán Norcán Odymp Ofaodie Ofaoib Olucky Osnaisre Ostas Ozsince Peart Peilín Peris Petenn Pilinny Pranouib Pranuel Prear Premmie Ptasaen Pádrus Póillaurt Póire Póirv Rande Rannearie Raxie Reagdagán Rikey Risty Ritrit Rodaw Roilf Roille Roindy Roinn Roire Rollainn Ronace Roswalbil Rotep Ruadam Ruait Rually Ruargus Ruarty Rudie Ruffaoil Ruilie Réalean Réark Rídín Róseb Saffaorty Safrael Safre Sally Sameilín Saonc Saorry Saoscammy Seard Searj Searnew Seogdabby Seteff Sianni Siasara Sielín Sinéanuel Sionaitz Sobnaird Solmás Solmón Spenna Stefe Stionaill Stíonank Suirike Séady Séalla Séarricky Sílew Taddy Tamende Tammy Tannán Teffy Temmy Tialas Tianaire Timik Timor Tinnán Tobby Toibasad Toifre Toiriele Tomen Tomert Tomiony Torre Trear Trewt Trino Tritz Tuait Uaigna Uainov Uaitlín Ualdo Uanniamen Uargie Uirse Valtán Wilidymp Wilie Wilim Winnel Woose Xavić Zannce Áiltán Áindy Árdge Árdia Éamay Éamear Éannie Éasán Éimmy Éiríose Éistóire Émenny Émenus Étann Únlutz

[itsnot365papers]

Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis.

Hamish Stewart, Dmitry Grinfeld, Anastassios Giannakopulos, Johannes Petzoldt, Toby Shanley, Matthew Garland, Eduard Denisov, Amelia Peterson, Eugen Damoc, Martin Zeller, Tabiwang N.Arrey, Anna Pashkova, Santosh Renuse, Amirmansoor Hakimi, Andreas Kühn, Matthias Biel, Arne Kreutzmann, Bernd Hagedorn, Immo Colonius, Adrian Schütz, Arne Stefes, Ankit Dwivedi, Daniel Mourad, Max Hoek, Bastian Reitemeier, Philipp Cochems, Alexander Kholomeev, Robert Ostermann, Gregor Quiring, Maximilian Ochmann, Sascha Möhring, Alexander Wagner, André Petker, Sebastian Kanngiesser, Michael Wiedemeyer, Wilko Balschun, Daniel Hermanson, Vlad Zabrouskov, Alexander Makarov and Christian Hock.

Pre-print, 2023

Summary/Figures:

Fig 1- schematic- astral uses orbitrap as intact mass analyzer, then ions are routes/guided through multipole and octapole into a high pressure region, then a low pressure region where they are ejected into the astral analyzer. ions then move back along the mirrors until they are fully reflected and detected i nthe front of the analyzer

key feature is that the high pressure chamber allows pooling of ions at the same time as that the low pressure chamber is ejecting them into the astral, enables much faster times than other mass analyzer setups

fig 2- timing schema for each section- 4 packets of ions can be in processing at once b/c steps are so fast but also separate

fig 3- MS1 in orbitrap can be done t the same time as MS2 in the astral

fig 4- resolution remains high across a wide mass range and at two different ion injection levels, mass shift remains low and consistent at wide mass range and two different ion injection levels. would be useful to see orbitrap data overlaid on this.

fig 5- mass jitter across m/z range is mostly consistent, it gets slightly higher. 3sigma jitter seems to basically be the 3x the standard deviation of the measured mas for the handful of peaks plotted here, so jitter is just a measure of deviation, (?)

fig 6- mass shift of a specified calibration peak over 70 hours is within 1m/z shift, text states that this was compared to an intemrittent internal calibration, but it is unclear how that would be done simultaneously and all of the data points for both masses are continuous

fig 7- the correction for temperature based on the internal calibrant keep mass error much closer to 0

fig 8- higher accumulation time in the in the trap results in lower scan rate- this is because a greater proportion of the duty cycle is occupied by ion accumulation leaving less total time for scans, however if the accumulation time is left constant then the scan frequency is constant

fig 9- comparison of ion countrs between corrected and uncorrected data, based on astral coutnrs relative to orib counts, where even the uncorrected astral counts are 100%- 150% higher

fig 10- astral spectra include more peaks for the same # of measured ions of a given compound, also shows that resolution values for the astral stay around 100k but orbi resolution is much lower

fig 11- protein group and peptide identification increase with experiment time

fig 12- proteome mixtures can be accurately identified using the astral, CV values for protein ID's are very low

fig 13- similar experiment, but with DIA on hela cells. Successfully identified many peptides and proteins both with and without libraries, increasing with loading mass (these are expected results)

Questions:

resolving power vs resolution?- resolution is the ability to separate peaks in a mass spectra (describes the peak themselves), whereas resolving power is the ability of the mass spec to separate the peaks (a property/parameter),

relation of repetition rate (Hz), voltage, resolving power, m/z

are the Asymmetric Ion Mirrors actual glass mirrors, or are they some other kind of "mirror"?- they are metallic, the "mirroring" is achieved via voltage

scintillator- type of mass detector that can measure ionization, traditionally used for radiation, this is a portion of the mass detection system in the astral

hermetically sealed- sealing to prevent both gases and moisture from entering (not the same as airtight)

jitter- noise within a specified domain (?)

mass dependent correction (reference 40)-

Why does an orbitrap require 10 ions for a peak to be measured but the astral can measure single ions?

Why start with the Astral at 500% aGC target? What experiments went into determining this was a good level

How was data for figure 6 and 7 collected?

Does instrument resolution refer to the average of all the resolution of each individual peak?

[gasstationtweaker]

gwa gwa

[autistickfigure]

Stefe before i go

[createmydreamblog]

„Sny mają niewyobrażalną moc i to od ciebie zależy czy zaczniesz je spełniać” Witam was w tym pięknym słonecznym dniu z 1 książka na luty ❤️ Oczywiście musi być w śnieżnym wydaniu 😍 ⭐⭐⭐⭐⭐⭐⭐⭐⭐⭐⭐⭐⭐ Poznajcie Stefe, która bała się gwiazd, ale przeżyła niesamowitą przygodę 🤩 Dziewczynka wraz z sarną, która ją odwiedziła poznaje strażniczkę gwiazd, która mówi, że Stefa jest jej następczynią. Kiedyś strażników bylo wielu, ale pomału umierali i została jedna. Gwiazdy wygasają bo otulają je czarne róże tęsknoty za zaginionymi, w związku z czym pomału niebo nie świeci tak jak dawniej. Dziewczynka podczas swojej podróży po magicznych krainach poznaje piękno i jednocześnie smutek oraz strach. Odkrywa prawdę i jednoczenie dostaje nowe zagadki do odkrycia, w tym zaginięcie syna sąsiada. Jednakże czy dziewczynka postanowi zostać strażnikiem czy tęsknota za bliskimi będzie większa niż chęć nowej przygody? Przekonajcie się sami, bo naprawdę warto. Razem z dziewczynką poznajcie czym jest poznanie, smutek, nadzieja. Przeżyjcie przygodę tak niesamowitą, że mogłaby okazać się snem niż prawdą. Poznajcie krainę widoczną tylko w wyobraźni lub te zniszczone przez czarne róże. Poznajcie miasto Słowa i rodzinną atmosferę ❤️ Przyznam, że wciągnęła mnie ta krótka historia o małej dziewczynce, z pewnością czyta się ją dość szybko ❤️ Trafiłam na nią przez przypadek i stwierdziłam, że z milą chęcią zobaczę o czym to jest. Można to czytać dzieciom lub samemu i na pewno jednemu i drugiemu przypadnie do gustu. Takie książki nie muszą być tylko dla dzieci. Jedno jest pewne dzieci i sny mają wielką moc. Dzieci nie doświadczyły tych wszystkich problemów, patrzą na świat inaczej i są bardziej otwarci na pomoc jak i na smutek ale dostrzegają więcej niż dorośli. Polecam gorąco, jak dla mnie 10/10 ⭐ #book #recenzja #blog #star #sky #night #journay #love #sad #scared #strażnicy #animals #rose #tęsknota #fight #dream #family #amazing #beautiful #książkowescrabble #stefaitysiącegwiazd #ninajaszewska #girl #pomoc #february #winter #snow #read (w: Chaberkowo) https://www.instagram.com/p/CoR3XD2IsDI/?igshid=NGJjMDIxMWI=

[mahekinteractive]

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[stefaniamolnar]

Happy national girlfriend day to the girls who have gotten cheated on, lied too, left with no explanation, emotionally abused, &’ left a toxic relationship. You females are strong independent young women! 📷 You deserve the world and if no one out here is willing to give it to you go get it yourself. You’re all beautiful!