#polynucleotide phosphorylase

[biology-geology-beaches-india]

TSRNOSS. Page 225.

[blogpostatron3000]

I love that it's not called polynucleotide polymerase and that i mashed the names of two separate enzymes together. I'm sorry polynucleotide phosphorylase. I'm sorry polyadenylation polymerase.

Sometimes biologists name things like. Polynucleotide polymerase. It's an enzyme that makes polynucleotide polymers. And sometimes biologists name things like H2A*H2B. And you just have to be okay with that.

[rnomics]

Defects of mitochondrial #RNA turnover lead to the accumulation of double-stranded #RNA in vivo

by Aleksandra Pajak, Isabelle Laine, Paula Clemente, Najla El-Fissi, Florian A. Schober, Camilla Maffezzini, Javier Calvo-Garrido, Rolf Wibom, Roberta Filograna, Ashish Dhir, Anna Wedell, Christoph Freyer, Anna Wredenberg The #RNA helicase SUV3 and the polynucleotide phosphorylase PNPase are involved in the degradation of mitochondrial #mRNAs but their roles in vivo are not fully understood. Additionally, upstream processes, such as transcript maturation, have been linked to some of these factors, suggesting either dual roles or tightly interconnected mechanisms of mitochondrial #RNA metabolism. To get a better understanding of the turn-over of mitochondrial #RNAs in vivo, we manipulated the mitochondrial #mRNA degrading complex in Drosophila melanogaster models and studied the molecular consequences. Additionally, we investigated if and how these factors interact with the mitochondrial poly(A) polymerase, MTPAP, as well as with the mitochondrial #mRNA stabilising factor, LRPPRC. Our results demonstrate a tight interdependency of mitochondrial #mRNA stability, polyadenylation and the removal of antisense #RNA. Furthermore, disruption of degradation, as well as polyadenylation, leads to the accumulation of double-stranded #RNAs, and their escape out into the cytoplasm is associated with an altered immune-response in flies. Together our results suggest a highly organised and inter-dependable regulation of mitochondrial #RNA metabolism with far reaching consequences on cellular physiology. http://bit.ly/31du4MJ

[rnomics]

Antibiotics, Vol. 7, Pages 25: Novel Aspects of Polynucleotide Phosphorylase Function in Streptomyces

Polynucleotide phosphorylase (PNPase) is a 3′–5′-exoribnuclease that is found in most bacteria and in some eukaryotic organelles. The enzyme plays a key role in #RNA decay in these systems. PNPase structure and function have been studied extensively in Escherichia coli, but there are several important aspects of PNPase function in Streptomyces that differ from what is observed in E. coli and other bacterial genera. This review highlights several of those differences: (1) the organization and expression of the PNPase gene in Streptomyces; (2) the possible function of PNPase as an #RNA 3′-polyribonucleotide polymerase in Streptomyces; (3) the function of PNPase as both an exoribonuclease and as an #RNA 3′-polyribonucleotide polymerase in Streptomyces; (4) the function of (p)ppGpp as a PNPase effector in Streptomyces. The review concludes with a consideration of a number of unanswered questions regarding the function of Streptomyces PNPase, which can be examined experimentally. http://bit.ly/2tTDb9C #MDPI