#oncology primer

[grits-galraisedinthesouth]

The Princess delivered such a clear & well written statement for the layperson. She displayed unparalleled poise, courage, and compassion under the harshness of the global spotlight.

I feel for her. Unlike Charles (IMO), Catherine never anticipated that she might have been battling cancer symptoms. Hearing the pathology results was devastating. They deserve our compassion.

And yet THIS:

Knowledge is Power

I already created a post to help the layperson better understand "preventative chemotherapy."

The use of preventative chemotherapy means Catherine's lymphatic system is concerning and warranted aggressive action to ensure that she is cured of this disease.

Sometimes a malignant tumor is removed and the body is cancer free [Sarah York/Guiliani Rancic].

Thankfully Catherine's magins are cancer free as there is no evidence the cancer metastasized.

Despite those cancer free margins, the cancer gained access to the lymph channels (not her lymph nodes), hence she was prescribed a course of a "preventative" form of poison (chemotherapy) to arrest & annihilate the wandering cancer cells in every system of her body. 😥

[blackbiobiotech]

Advancing Cancer Diagnosis using 3B BlackBio Biotech's MGMT Methylation Detection Kit

In the realm of cancer diagnostics and treatment, precision and accuracy are paramount. One of the critical factors influencing treatment decisions in oncology is the methylation status of certain genes. Among these, the O^6-methylguanine-DNA methyltransferase (MGMT) gene stands out for its role in DNA repair and its impact on the effectiveness of chemotherapy. 3B BlackBio Biotech has developed an innovative solution to assess this important biomarker: the MGMT Methylation Detection Kit. This cutting-edge tool is revolutionizing the field of cancer diagnostics by providing reliable and precise detection of MGMT promoter methylation.

The Role of MGMT in Cancer

The MGMT gene encodes a DNA repair protein that removes alkyl groups from the O^6 position of guanine, a process crucial for maintaining genomic integrity. In many types of cancer, the promoter region of the MGMT gene becomes methylated, silencing the gene and reducing the production of the MGMT protein. This loss of function can be beneficial in certain chemotherapy regimens, as it renders cancer cells more susceptible to the DNA-damaging effects of alkylating agents, such as temozolomide. As a result, the methylation status of the MGMT promoter is a valuable biomarker for predicting patient response to specific treatments.

3B BlackBio Biotech's MGMT Methylation Detection Kit

3B BlackBio Biotech's MGMT Methylation Detection Kit utilizes real-time polymerase chain reaction (PCR) technology to accurately and efficiently determine the methylation status of the MGMT promoter. The kit includes highly specific primers and probes designed to differentiate between methylated and unmethylated alleles, ensuring precise detection.

The process begins with bisulfite conversion, a step that modifies unmethylated cytosines into uracil while leaving methylated cytosines unchanged. This change allows for the PCR amplification to clearly tell the difference between methylated and unmethylated DNA. The real-time PCR component of the kit then quantifies the methylation levels, providing a comprehensive analysis of the MGMT promoter's methylation status.

Key Features and Benefits

1. High Sensitivity and Specificity: The MGMT Methylation Detection Kit is designed to detect even low levels of methylation, making it highly sensitive. The use of specific primers and probes enhances the accuracy of detection, ensuring reliable results.

2. User-Friendly Protocol: The kit's streamlined protocol simplifies the detection process, allowing for quick and efficient analysis. This ease of use is beneficial for both clinical laboratories and research settings.

3. Comprehensive Package: The kit includes all necessary reagents and controls, providing a complete solution for assessing MGMT promoter methylation.

4. Reproducible Results: The MGMT Methylation Detection Kit offers consistent and reproducible results, which are crucial for making informed clinical decisions.

Applications in Oncology

The detection of MGMT promoter methylation has significant clinical implications. For patients with gliomas and other cancers, knowing the methylation status of the MGMT gene can guide treatment planning. Specifically, patients with methylated MGMT promoters are more likely to benefit from alkylating agents, as their cancer cells have a diminished capacity to repair DNA damage. This information can lead to more personalized and effective treatment strategies, improving patient outcomes.

Conclusion

The 3B BlackBio Biotech MGMT Methylation Detection Kit is a notable advancement in the field of cancer diagnostics. By providing a reliable and accurate method for detecting MGMT promoter methylation, the kit enables healthcare professionals to make more informed treatment decisions. This not only enhances the precision of cancer therapy but also contributes to the broader understanding of cancer biology.

For more information about the MGMT Methylation PCR and other innovative products, visit at https://3bblackbio.com/trupcr-oncology-mgmt-methylation-detection-kit.html. With a commitment to advancing cancer diagnostics and treatment, 3B BlackBio Biotech continues to lead the way in developing cutting-edge solutions for the healthcare industry.

[shawntillitt]

A Primer on Pediatric Cancer

Each year, 400,000 children worldwide receive a cancer diagnosis. Pediatric cancer claims more young lives in the US than any other disease. Medical professionals diagnose childhood cancers at age six, although they can affect children and teenagers of all ages.

Pediatric and adult cancers differ from each other. Cancers that affect children exhibit distinct behaviors compared to those seen in adults.

Unlike adult cancers, behavior, and lifestyle rarely play a role in why a child develops cancer. However, the consequences of treatment can have lifelong impacts on them. Delayed effects such as mental health issues, memory loss, hearing loss, organ damage, infertility, nerve damage, and stunted bone growth have become common among childhood cancer survivors.

There are over a dozen types and numerous subtypes of pediatric cancers. Common pediatric cancers include leukemia, brain and spinal cord tumors, neuroblastoma, Wilm's tumor, and other cancers that affect the kidneys and bones.

Common childhood cancer symptoms resemble childhood illnesses, such as headaches, stomach aches, and minor injuries like bumps and bruises. Additional potential childhood cancer symptoms include weight loss, swelling or pain in bones, joints, or other areas, lumps or masses, excessive bruising or bleeding, frequent infections, changes in eye appearance, persistent nausea or vomiting, fatigue or paleness, sudden eye or vision changes, and recurring fevers of unknown origin; discuss persistent or worsening symptoms with a healthcare provider.

Medical professionals diagnose childhood cancers through various tests based on age, overall health, and symptoms. They use blood tests to diagnose conditions like leukemia and lymphoma, biopsies to examine tissue samples, bone marrow biopsies to detect bone marrow issues, lumbar punctures to collect cerebrospinal fluid for cancer cell detection, ultrasounds, CT scans, MRIs, and PET scans combined with CT scans for imaging organs and tissues.

The primary factors influencing treatment for childhood cancer are type and stage. Common treatment modalities include surgery, radiation therapy, and chemotherapy (chemo). Cancer treatments developed for adults may not effectively address the needs of children as their growing bodies respond differently to medications. Striking a balance between intensive treatment and minimizing side effects is crucial for medical practitioners who are treating children with cancer.

In some cases, specialists use multiple treatment modalities to combat childhood cancers, such as high-dose chemotherapy followed by a stem cell transplant. Additionally, emerging treatments like targeted therapy drugs and immunotherapy are gaining significance in managing specific childhood cancers.

Typically, chemotherapy shows greater treatment efficacy due to the rapid growth rate of cancer in children. Children's bodies may withstand higher doses of chemotherapy compared to adults. While intensive treatments enhance the chances of cancer remission, they can also result in increased short- and long-term side effects.

Radiation therapy can be effective as a cancer treatment but may pose heightened risks for children, particularly young ones. To minimize adverse effects, medical professionals take several precautions before employing it.

Specialized teams at centers dedicated to treating children's cancers address the unique needs of their pediatric patients and their families. The interdisciplinary teams consist of experts well-versed in the distinctions between adult and childhood cancers and the specific requirements of children undergoing cancer treatment. Typically, the team includes pediatric oncologists, pediatric surgeons, radiation oncologists, pediatric oncology nurses, nurse practitioners (NPs), and physician assistants (PAs). Children's cancer centers may offer additional support through psychologists, social workers, child life specialists, nutritionists, rehabilitation and physical therapists, and educators to ensure comprehensive care and assistance for the entire family throughout the treatment journey.

Survival rates for childhood cancer can vary depending on the type of cancer. Due to advancements in treatments customized to their requirements, over 80 percent of children and adolescents survive five years after receiving a cancer diagnosis.

[hospitalstore]

Unveiling the DNA Detective: A Look at Thermal Cyclers and Their Role in Molecular Biology

The building blocks of life, DNA, hold the secrets to our biology, health, and even our history. Molecular biology has emerged as a powerful field, unlocking these secrets through various techniques. One of the most fundamental tools in this realm is the thermal cycler, often simply called a PCR machine (polymerase chain reaction). This blog will explore the fascinating world of thermal cyclers, delving into their role in amplifying DNA and how they are revolutionizing various fields of science and medicine.

Beyond the Microscope: The Power of Thermal Cycling

Unlike microscopes that allow us to see biological structures, thermal cyclers work on a molecular level. They utilize a precise temperature control mechanism to facilitate DNA replication millions of times, a process known as PCR. Here's a simplified breakdown:

Sample Preparation: Isolated DNA containing the target sequence is placed in a reaction tube along with primers (short DNA fragments), DNA polymerase (an enzyme that builds new DNA strands), and necessary chemical components.

Thermal Cycling: The thermal cycler precisely regulates temperature changes through multiple cycles. These cycles typically involve denaturation (high temperature to separate DNA strands), annealing (lower temperature for primers to attach to specific target sequences), and extension (optimal temperature for DNA polymerase to synthesize new complementary DNA strands).

Amplification: With each cycle, the target DNA sequence is replicated, resulting in an exponential increase in copies. Millions of copies can be generated from a tiny starting amount of DNA.

Applications of Thermal Cyclers: Transforming Research and Medicine

Thermal cyclers are versatile workhorses used across various scientific disciplines:

DNA Cloning: Amplified DNA can be inserted into plasmids (circular DNA molecules) used for further analysis or genetic engineering applications.

Genetic Testing: Thermal cyclers are vital for detecting genetic mutations, diagnosing inherited diseases, and identifying pathogens like viruses and bacteria.

Forensic Science: DNA profiling from crime scene evidence utilizes thermal cyclers to amplify trace amounts of DNA for identification purposes.

Oncology Research: Cancer research heavily relies on thermal cyclers to study genetic mutations involved in tumor formation and progression.

The Ever-Evolving World of Thermal Cyclers

Thermal cycler technology is constantly advancing:

Real-Time PCR: These machines monitor DNA amplification as it occurs, providing real-time data for faster analysis.

High-Throughput Screening: Automated systems allow parallel processing of multiple samples, increasing efficiency for large-scale studies.

Miniaturization: The development of compact thermal cyclers facilitates DNA testing at the point of care in clinical settings.

Thermal cyclers are indispensable tools in molecular biology research and diagnostics. By amplifying DNA, these machines enable scientists and medical professionals to diagnose diseases, understand biological processes, and develop new therapeutic approaches. As technology continues to evolve, thermal cyclers will undoubtedly play an even more significant role in shaping the future of medicine and scientific discovery.

When selecting thermal cyclers, research institutions and laboratories should partner with reputable suppliers like HospitalStore to ensure they receive high-quality, reliable equipment backed by excellent customer service and technical support.

[skreddy]

A Comprehensive Guide: List of Scopus Journals for Academic Research

Introduction:

List of Scopus Journals, one of the world's largest abstract and citation databases, plays a crucial role in academic research. It provides access to a vast collection of high-quality scholarly journals across various disciplines. Researchers, academicians, and students often rely on Scopus to access reputable journals for their research endeavors. In this article, we present an extensive guide listing some noteworthy journals indexed in List of Scopus Journals, categorized by academic fields.

Science and Technology:

Journal o

f Applied Physics

Journal of Environmental Management

Materials Science and Engineering: A

Computer Physics Communications

Applied Energy

Social Sciences:

Journal of Organizational Behavior

Social Science & Medicine

Psychology of Addictive Behaviors

Political Geography

Education for Sustainable Development

Health and Medicine:

The Lancet

Annals of Internal Medicine

Of Clinical Oncology

Nature Reviews Disease Primers

Vaccine

Business and Economics:

Journal of Business Research

Harvard Business Review

Journal of Financial Economics

Management Science

Journal of Marketing

Arts and Humanities:

Journal of Aesthetics and Art Criticism

Theatre Research International

Art History

Cultural Studies

Language and Literature

Engineering:

Engineering Structures

Journal of Mechanical Engineering Science

Robotics and Autonomous Systems

Journal of Electrical and Electronic Engineering

Sustainable Energy Technologies and Assessments

Environmental Science:

Environmental Science and Technology

Journal of Environmental Sciences

Environmental Pollution

Water Research

Ecology Letters

Mathematics:

Journal of Mathematical Analysis and Applications

Advances in Computational Mathematics

Mathematical Reviews

Linear Algebra and its Applications

Mathematics and Computers in Simulation

Conclusion:

This article has presented a comprehensive guide listing notable List of Scopus Journals indexed in Scopus across diverse academic fields. Access to these journals is vital for researchers, scholars, and students seeking reliable and credible sources for their academic pursuits. It's important to explore and utilize these journals to enhance the quality and rigor of academic research.

Hyderabad, Telangana

#research #papers #publish

[naresh12-08-2002]

 Navigating the Landscape of Medical Research Journals : A Comprehensive Review of  Medical Scopus Journals

Introduction:

In the ever-evolving field of medical scopus  journals , staying abreast of the latest scientific advancements is crucial. Scopus, a widely recognized abstract and citation database, plays a pivotal role in this regard. This article delves into the realm of medical research as documented in Scopus-indexed journals, providing an overview of the key publications and their contributions to the field.

Understanding Scopus:

Scopus stands out as a comprehensive database that encompasses a vast array of scientific disciplines, with a particular emphasis on medicine. It serves as a repository for scholarly articles, conference papers, and patents, making it an invaluable resource for researchers, academicians, and healthcare professionals.

Key Features of Scopus:

Extensive Coverage: Scopus boasts an extensive collection of medical journals, covering diverse areas such as clinical medicine, public health, pharmacology, and biomedical sciences. This breadth ensures that researchers have access to a wide range of topics within the medical domain.

Global Reach: One of the strengths of Scopus is its global coverage. Journals from around the world contribute to the database, fostering an international perspective on medical research. This global inclusivity enhances the richness and diversity of the content available.

Citation Analysis:Scopus provides citation metrics, allowing researchers to assess the impact and influence of specific articles. This feature aids in identifying seminal works and understanding the interconnectedness of research in the medical field.

Interdisciplinary Insights:As medical research often intersects with other disciplines, Scopus offers interdisciplinary insights by indexing journals from related fields. This interdisciplinary approach encourages collaboration and the synthesis of diverse perspectives.

Prominent Scopus-Indexed Medical  Journals:

The Lancet: A renowned medical scopus journals , The Lancet, consistently publishes high-impact research across various medical specialties. Its contributions range from clinical trials to public health policy, making it a cornerstone of evidence-based medicine.

Journal of Clinical Oncology: Focused on cancer research and treatment, this journal is a vital resource for oncologists, researchers, and healthcare professionals. It covers groundbreaking studies, treatment modalities, and advancements in cancer care.

New England Journal of Medicine (NEJM): NEJM is a prestigious publication that disseminates cutting-edge research in internal medicine. Widely regarded for its rigor and impact, NEJM is a go-to source for physicians and researchers worldwide.

Nature Reviews. Disease Primers:Combining the rigor of scientific research with comprehensive reviews, this journal provides primers on various diseases. It serves as an excellent resource for understanding the fundamental aspects of medical conditions.

Conclusion:

Navigating the vast landscape of medical scopus  journals is a complex task, but Scopus serves as a guiding light. Its extensive coverage, global reach, and emphasis on interdisciplinary insights make it an indispensable tool for researchers in the medical field. By exploring the wealth of knowledge within Scopus-indexed journals, professionals can stay at the forefront of medical advancements and contribute to the ongoing progress of healthcare worldwide.

27 Old Gloucester Street London, England.

#Research #papers #publish

[pathologylab]

#Genes2Me offers the #BRCA detection #IVD kit which is specifically designed for accurate detection of BRCA1 and BRCA2 #gene mutations associated with breast and #ovarian_cancers. Utilizing specific primers and probes, our #kit ensures error-free targeting and identification of gene #mutations.

Contact us today to learn more about our BRCA-Q #RT_PCR IVD Kit or visit our website for more information. https://www.genes2me.com/ivd-real-time-pcr-test-kits

#oncology #pcrtesting #madeinindia #breastcancer #cancer #testing #screening #manufacturer #brca1 #brca2 #pcr #diagnostic #invitro #cancercare

[covidclinic]

All You Need to Know About RT-PCR Testing

In the medical world, the stakes are high. At times, lives hang in the balance. That’s why it’s important to have a safe and reliable testing procedure in place to ensure a positive diagnosis. But what is RT-PCR? How much does it cost? And how do you know if it’s right for your needs? We've gathered five pros and cons that will help you make that decision.

What is RT-PCR?

RT-PCR stands for a Reverse Transcriptase-Polymerase Chain Reaction. At its most basic, RT-PCR is a technique used to amplify a small amount of genetic material into a larger amount. It’s used in gene expression studies, where you want to study the expression levels of a gene in a cell. Because as you amplify the genes, proteins build up on the outside of the cell, and you can then analyze those proteins to find out the expression levels that are associated with certain diseases. Common diseases to which RT-PCR is typically used are asthma, diabetes, cancer, immune response, and in today’s times, COVID-19.

The downside of RT-PCR, it most likely, is price. It typically takes between $1,000–$3,000 to amplify a specific gene to a higher level. This is where a third option comes into play: You can also use the reverse-RT-PCR technique that amplifies your genetic material to a lower level. For example, reverse-RT-PCR is what’s used in AAV-7 viral vector cocktails. Prices for this are pretty affordable too.

FAQ: Q: Is it necessary to choose one or the other if you can do both? A: RT-PCR and reverse transcriptase are usually used together, but not always. Usually, it isn’t necessary to use both. This can be based on what you’re trying to achieve, such as if you���re trying to prevent a certain disease or increase your metabolic function. Q: What does the cost per unit of amplification go toward? A: Fundamentally, these processes cost money. The higher the amount you amplify, the more expensive it will be. At the same time, though, the more expensive the process, the less effective it’ll be. Since whole-genome amplification typically costs $2,500–$5,000, we can see that for an initial test, $5,000 would be the most cost-effective. As you increase that amount, you’ll only have to pay a fraction of the total cost.

Pros and cons of RT-PCR: Reverse transcription PCR (RT-PCR), also known as PCR amplification of cDNA, is a laboratory technique for amplifying nucleic acids from an RNA template, such as from total RNA or a transcriptome library. The term "reverse transcription" refers to the process of producing a complementary DNA (cDNA) copy of an mRNA template using the enzyme reverse transcriptase.  In this process, synthetic DNA is introduced and is copied exactly as it was produced. A selection step then occurs, where the reverse transcriptase molecule recognizes and amplifies a polyadenylated cDNA sequence in the target mRNA, allowing it to be translated into a new mRNA molecule and the cycle starts all over again. In RT-PCR, two types of primers (secondary structures for the enzyme reaction) are used: one is designed to amplify specific, often-coding splice variants of the mRNA, and the other is designed to amplify both strands of the mRNA at the same time. This is done by using complementary sequences of amino acids along with specific control sequences present on the complementary sequence to form the template for the reverse transcriptase reaction. The primary purpose of RT- PCR is safety. PCR amplifies only the non-coding parts of the mRNA, such as 3′ untranslated regions (UTRs), and does not use any PCR products to amplify the coding regions, which are known as coding exons.Furthermore, the procedure does not change the amino acids that code for those particular amino acids, so there is no chance that a false-positive result could occur. According to the American Society for Clinical Pathology, RT- PCR is just as accurate as of the traditional enzyme-linked immunosorbent assay (ELISA) and will most likely identify the type of cancer you have, as well as confirm your immune system status. However, RT-PCR is not as fast as true-positive ELISA, which gives you an indication of whether or not you have cancer. Under some circumstances, however, the RT-PCR could be more accurate than the ELISA.Nonetheless, the Portuguese Society of Clinical Oncology warns that if the results are negative, you’re most likely dealing with an occasional false-positive.

How is RT-PCR Performed?  RT-PCR is a technique used to amplify a single copy of a piece of DNA to detectable levels. It is used to detect a viral or bacterial infection, or to detect the presence of specific RNA molecules.

What Is It and What Does It Do? RT-PCR can be performed on any sample of blood or tissue, including, but not limited to, whole blood after surgery, urine, tissue or hair, or cerebrospinal fluid (brain or spinal fluid). Alternatively, it can be performed on specific pieces of genetic material by adding a specific piece of copied DNA to a reaction mixture, which is heated and allowed to run for a specific amount of time. Depending on what you need, RT-PCR Test can cost from $100–$1,000.

What’s Changed? More studies have been done with RT-PCR, and the current standard for diagnosing infections is a quantitative RT-PCR (qRT-PCR) test, which uses an expensive, highly skilled lab worker to analyze your DNA. Due in part to these high costs, more studies are now using what is called a specific oxidative burst DNA test (a polymerase chain reaction test), in which relatively small amounts of DNA are paired with a specific piece of viral or bacterial genetic material and the reaction is amplified, much like a fire. In this case, the fire is a person going through the heavy exercise, causing damage to their DNA. This type of DNA amplification is much quicker and less cumbersome, and, in some cases, simpler.  Many doctors utilize qRT-PCR because it is cost-effective and easy, and more studies are starting to investigate specific pathogens other than COVID-19. If you have another inherited disorder, a certain enzyme can be tested to more quickly determine the prevalence of the condition and its impact on your body.However, using RT-PCR does not guarantee a positive diagnosis and it can, in rare cases, falsely diagnose infection or disease.

Where can it be Performed? The main place where PCR is used is in the workplace, but it is also used in the field of education and in self-development.In essence, it’s: “Do you have a problem? Let’s test you.”The reason it is valuable in the workplace is because we are dealing with people’s basic human rights — safety, health, equality, and so on. These are things we can’t do without, and the current test is so slow it doesn’t allow us to make critical decisions.People also aren’t going to the doctor’s office, so we must screen them before they visit — which is where the RT-PCR process comes in.So, it’s an essential part of any clinical management — you can imagine the profoundly important difference in your patient-doctor relationship when that process does the screening. It comes at a price! Professional laboratory services for delivery of diagnoses are often priced at $10,000, $50,000, $100,000 and more.

RT-PCR Uses:

To figure out what to consider, let’s start by listing the things RT-PCR checks for:Oxygen saturation (ensures that the body can receive and process oxygen)Red blood cell countPlatelet countsSerum lipid profilesCritical Mass Values (effects of different medications)And in today’s situation, COVID-19. Here is the list of services that you can choose from. We’ll try to offer you insights into the details, talk about the cost and draw some conclusions.

Types of Testing: There are really only two main types of RT-PCR: serological and biochemical. Serological testing is more expensive and focuses on specific blood markers; it doesn’t necessarily include other tests as biochemical testing can integrate additional tests into the process, such as a genetic analysis.

So Concluding This, Who Can Use These Tests?

State and municipal public health laboratories in the United States, as well as Department of Defense laboratories, receive CDC tests if they have been previously qualified to perform a similar type of test used to detect influenza or have recently been approved for SARS-CoV-2 testing by their state public health laboratory.

[pharmdup]

Hi :) Do you have any Pharm resources for someone involved in clinical trials who would like to know more? So biochem/pharmacology related books, articles, videos, podcasts?

That’s such a great and big question! I just compiled a list of links to resources that I use at least weekly, but I don’t know if it will be what you’re looking for. I like reading more than podcasts for drug updates, but I hear pharmacyjoe’s podcast is pretty good. If you want a quick primer into how a disease is currently treated, I recommend seeing if your academic library subscribes to the DynaMed database.

The best intro-to-pharmacy textbook on how to be a pharmacist is DiPiro’s Pharmacotherapy. In practice, pharmacists don’t often look at the studies on biochemistry in depth, because that was often established decades ago. We go to databases like Lexicomp, which is expensive, but Drugs.com is almost as useful and free. Martindale’s Complete Drug Reference might be the closest resource that would be useful to both you and me.

I’ll include the list of links for places that I currently follow that I haven’t linked already. Many of them cost money in some way, but email literature updates for free.

I hope other pharmacists add their two cents! I went a bit out in the weeds on this question, but had fun answering it. Thanks!

[saucysayswhat]

Obtenga más información sobre la colposcopia

¿Qué sucede después de una prueba de Papanicolaou anormal?

¿Quieres protegerte del cáncer de cuello uterino? Su primer paso es hacerse una prueba de Papanicolaou con regularidad. De esa manera, si tiene células precancerosas, se detectan temprano.

El cáncer de cuello uterino generalmente comienza como una condición precancerosa llamada displasia, que puede detectarse mediante un Papanicolaou y es 100 por ciento tratable, si se detecta a tiempo.

Pero, ¿qué sucede si su Papanicolaou regresa con resultados anormales? James Pavelka MD, un oncólogo ginecológico de Tristate Gynecologic Oncology, analiza lo que sucede a continuación y por qué no debe entrar en pánico.

Resultados anormales de la prueba de Papanicolaou: ¿Qué significan?

"Es importante recordar que una prueba de Papanicolaou anormal es una experiencia bastante común para muchas mujeres", explica la Dra. Pavelka.

Un Papanicolaou anormal generalmente significa que ha habido cambios leves en las células del cuello uterino. Los resultados pueden significar uno de los siguientes:

– Hay células atípicas de significado incierto. – Los cambios pueden deberse al virus del papiloma humano. – Hay cambios que pueden conducir al cáncer.

Sin embargo, las pruebas de Papanicolaou solo detectan cambios alrededor del 60 por ciento de las veces, "por lo que es importante repetir las pruebas de Papanicolaou con el tiempo", señala. "No solo pasa por alto cosas que podrían estar allí, sino que a veces la inflamación o las infecciones leves pueden crear la apariencia de cambios precancerosos cuando en realidad no hay ninguno".

Obtenga más información sobre los resultados de la prueba de Papanicolaou. Obtenga más información sobre el virus del papiloma humano.

"Recibí una prueba de Papanicolaou anormal. ¿Qué sigue?"

Su próximo paso suele ser un procedimiento menor llamado colposcopia. Este procedimiento es un examen visual del cuello uterino usando un microscopio de baja potencia que se usa para encontrar y luego hacer una biopsia de áreas anormales en el cuello uterino que pueden provocar cáncer de cuello uterino.

"La prueba de Papanicolaou es realmente solo una prueba de clasificación que nos dice quién podría usar una colposcopia y quién no", dice el Dr. Pavelka.

Obtenga más información sobre la colposcopia.

Preparación para una colposcopia

Las colposcopias no requieren ninguna preparación especial. Sin embargo, el Dr. Pavelka dice que al menos 24 horas antes del examen:

– No te duches. – No coloque ningún producto, como tampones, en la vagina. – No tener sexo. – Informe a su médico o enfermera si está embarazada o pudiera estarlo.

También dice que evite programar su colposcopia para un día en el que pueda estar en su ciclo menstrual. "Puede ser más inteligente cambiar la cita a un momento en el que no haya sangrado, solo porque eso podría oscurecer lo que podemos ver".

Durante el Procedimiento

Inicialmente, su ginecólogo insertará un espéculo, similar al que se usa durante una prueba de Papanicolaou, en la vagina. A continuación, aplicará un par de soluciones diferentes para ayudar a resaltar cualquier cambio precanceroso.

La primera solución es una solución de vinagre fuerte, que se usa para eliminar la mucosidad que cubre la superficie y resalta las áreas anormales. Su médico también puede aplicar una segunda solución, que es una solución de yodo, para identificar cualquier anomalía.

"Cuando observamos el cuello uterino… a menos que sepamos con certeza que algo es benigno, debemos realizar biopsias para ayudar a obtener un diagnóstico real de cuáles pueden ser los cambios en el cuello uterino", explica el Dr. Pavelka.

Después del procedimiento

Después de la colposcopia, no es raro que una mujer experimente una pequeña cantidad de drenaje o manchado vaginal. "A veces hay un poco de secreción relacionada con las soluciones utilizadas para resaltar las células anormales", señala el Dr. Pavelka.

Una solución en particular, llamada solución de Monsel, puede dejar una secreción de color marrón oscuro, parecida a los posos de café.

A partir de ahí, el ginecólogo enviará las biopsias a un patólogo. "Después del examen colposcópico, en términos generales, tendríamos una discusión… sobre los hallazgos, el momento de la patología y el posible tratamiento que ocurriría en función de los posibles resultados que pudiéramos obtener", dice.

Los resultados de la biopsia suelen tardar de una a dos semanas. Recibirá una llamada telefónica de seguimiento del consultorio de su ginecólogo para analizar los resultados, o es posible que su médico prefiera consultar en persona.

Aquí hay más información sobre colposcopía eche un vistazo a nuestra página web.

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[pranalipawarshinde]

Cell and Gene Therapy Market Trends and Competitive Analysis by 2019-2027

Cell and Gene Therapy Market: Introduction

Cell and gene therapy has high potential to eliminate and prevent several chronic disorders as well as life-threatening disorders especially cancer, heart diseases, AIDS, cystic fibrosis, and age-related disorders. The therapy provides complete cure to patients affected with chronic disorders rather than ease of symptoms offered by other treatments.

  The gene therapy concept emerged in 1989. Since then, researchers have been attempting to leverage the potential of gene therapy for treatment of rare and genetic diseases as well as diseases for which complete cure is not available yet.

  The first gene therapyproduct ‘Gendicine’ was developed by Shenzhen SiBiono GeneTech Co. Ltd. in 2003. It was commercialized for the treatment of head and neck cancer in 2004. Since 2004, more than 30,000 patients in China have been treated with Gendicine. Gendicine has exhibited exemplary safety results. When gene therapy is combined with other treatments, such as chemotherapy and radiotherapy, the overall outcome outperforms other standard therapies used alone.

 Report Overview:https://www.transparencymarketresearch.com/cell-gene-therapy-market.html

 Key Drivers and Restraints of Global Cell and Gene Therapy Market

Technological advancements in cell and gene therapy is one of the key factors projected to fuel the global cell and gene therapy market during the forecast period. Emerging technologies in cell and gene therapies, such as proprietary cell lines, gene vectors, cell expansionand separation systems, and single-use bioprocessing reactors, have become primary means by which single products have been transformed into a robust product portfolio. For example, Immunicum has offered three technological platforms i.e. gene editing, CAR-T cell expansion, and T-cell primers.  These technologies allow the company to advance its series of immuno-oncology drug candidates. Immunicum has two technologies i.e. IMM-2 platform and IMM-3 platform undergoing preclinical studies for use in the treatment of different types of cancer. Immunicum is looking forward to developing an allogeneic dendritic cell in-vivo vaccine for use in the treatment of solid tumors.

  Leading players operating in the immuno-oncology field have formed around 40 partnership programs regarding high-value cell and gene therapies. Thus, development of various technological platforms across a wide range of disease indications is one of the key factors driving the global cell and gene therapy market.

Cell and gene therapy is one of the most expensive treatments available in the world. High costs of cell and gene therapy and limited reimbursements are preventing patients from receiving treatments.

  For instance, Spark Therapeutics Inc.'s gene therapy product ‘Luxturna’ used for the treatment of retinal dystrophy is priced at US$ 850,000 per treatment. It is the world’s most expensive drug. Orchard Therapeutics’ gene therapy product ‘Strimvelis,’ which is used for the treatment of ADA-SCID is priced around US$ 648,000 per treatment.

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  Oncology Segment to Lead Global Cell and Gene Therapy Market

Approval of new gene therapy products and growth potential of gene therapy have led to need for increase in the number of treatment centers in the U.S. as well as Europe. Market players, after the initial success of their gene therapies, are striving to increase the number of treatment centers to extend the access to a larger pool of patients requiring gene therapy.

  In the first year of approval of Yescarta, a drug by Gilead, i.e. in 2017, only 15 authorized cancer hospitals were operating in the U.S. By the end of December 2017, only five patients were treated with Yescarta, whereas the waiting list had more than 200 patients. In October 2018, Gilead planned to increase the number of gene therapy centers to 90. As of June 2018, around 68 treatment centers were operating in the U.S. Also, the company has launched the ‘Kite Connect’ program to provide information and assistance, including insurance benefits, throughout the Yescarta therapy process.

  A gene therapy product called Kymriah, which was approved in 2017 for use in the treatment of patients aged up to 25 years and affected with large B-cell lymphoma, has only 41 treatment centers in the U.S. This is limiting a significant number of patients in the country from receiving gene therapy as a treatment.

 Key Players Operating in Global Cell and Gene Therapy Market

Manufacturers in the global cell and gene therapy market are increasingly investing in research and development of new and innovative techniques to manufacture advanced cell and gene therapies. These players are also focused on offering highly efficient and reliable products to provide better protection from fragility. The global cell and gene therapy market is less fragmented, with the presence of a small number of regional players. Market players focus on strategic partnerships, collaborations, mergers, acquisitions, and new product launches to gain higher market share. In December 2019, Merck signed a license agreement with Promega Corp. to provide the former’s foundational CRISPR intellectual property to the latter. As per the agreement, Promega would be using Merck’s CRISPR genome editing technology to develop research products and services, including those for drug development.

 Leading companies operating in the global cell and gene therapy market are:

  Biogen

Gilead Sciences, Inc.

Amgen, Inc.

Novartis AG

Orchard Therapeutics Plc

Spark Therapeutics, Inc. (A Part of Hoffmann-La Roche)

Molmed S.P.A.

Anges, Inc.

Bluebird Bio, Inc.

Human Stem Cells Institute (HSCI)

SIBIONO Genetech Co., Ltd.

Shanghai Sunway Biotech Co., Ltd

Uniqure N.V.

Gensight Biologics S.A.

Celgene Corporation (A Bristol-Myers Squibb Company) 

 Global Cell and Gene Therapy Market: Research Scope 

 Global Cell and Gene Therapy Market, by Application

Cancer

Autoimmune Diseases

Cardiovascular Disorders

Ophthalmology

Central Nervous System

Orthopedic Disorders

Others 

 Global Cell and Gene Therapy Market, by Therapy

Cell Therapy

Allogeneic

Blood Cells

Others

  Autologous

Human Embryonic Stem Cells

Hematopoietic Stem Cells

Mesenchymal Stem Cells

Others

  Gene Therapy

Non-viral Vectors

Viral Vectors

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[articlepublication]

Significance of DNA Library Normalization for NGS and Options Available

Normalization in NGS or Next-Generation Sequencing refers to equalizing the concentration of DNA libraries for multiplexing.

Multiplexing enables you to run multiple libraries on a single flow cell and reduces costs by maximizing the use of the ever-increasing capacity of NGS.

The lower DNA sequencing costs have made it possible to use in a wide range of molecular diagnostics applications, including reproductive health and oncology, as well as clinical research.

Uneven library concentrations from different qualities and quantities of samples can lead to inconsistent data. Libraries with a high concentration may be overrepresented on the flow cell and vice-versa. Hence, normalization is essential to ensure every library is equally represented and sequenced to sufficient depth.

Without normalization, you may have to spend additional time re-preparing libraries, if the sample is available. You would rather use this time on downstream analysis or preparing the next batch of libraries.

In addition, if your analysis is based on incomplete or inaccurate data, it will lead to missing out on key information, unreliable results or you would have to repeat experiments.

Comparing DNA quantitation and normalization methods

There are various options for quantitating library preps that vary inaccuracy.

a. Spectrophotometry - detects the absorption of UV light by molecules in the sample.

Advantages: Quick, low cost

Disadvantages: Inaccurate, measuring all nucleic acids, not just adaptor-ligated molecules; not very sensitive; affected by contaminating RNA and proteins

b. Electrophoresis - estimates fragment sizes through capillary electrophoresis and concentration through intercalating dyes.

Advantages: Accuracy in fragment size and distribution estimation

Disadvantages: Quantitation cannot differentiate between adaptor-ligated and other molecules; expensive equipment requirements for a single purpose

c. Fluorometry - uses fluorescent dyes to assess the concentration of nucleic acids against a standard curve.

Advantages: Sensitive and accurate estimation of dsDNA concentration; can be used to specifically quantitate single-stranded DNA, RNA, and protein; fast; low cost

Disadvantages: Cannot differentiate between adaptor-ligated and other molecules; not able to estimate fragment size

d. Quantitative PCR (qPCR) - adaptor-specific primers are used with fluorescent dyes and quenchers to quantitate library preps against standard curves.

Advantages: Accurate quantitation of adaptor-ligated molecules (viable sequencing templates); High sensitivity (suitable for quantitation of dilute libraries); Amenable to automation

Disadvantages: High cost; requires more hands-on time

While no single method provides all the data and accuracy you need for normalization, qPCR seems to offer the most accuracy in quantitation.

Magnetic beads-based normalization

Imagine that you didn’t need to go through quantitating your libraries at all!

Magnetic beads are already being used as a reliable and established way to safely handle nucleic acids.

Magnetic bead-based normalization is based on the principle that a given volume of beads can bind a consistent quantity of nucleic acid molecules. All unbound molecules are then washed away and each library is represented by just the bead-bound molecules.

Different coating options are available to suit various applications. For example,

a. carboxyl- and silica-coated magnetic beads can be used for generic, non-specific binding based on buffer conditions

b. oligo(dT)-coated beads for binding mRNA

c. streptavidin-coated beads for binding biotinylated samples

Studies indicate that bead-based normalization allows more consistent read depth than other quantitation-based methods.

Are you looking for effective and reliable magnetic bead-based kits for the normalization of DNA concentration? Call MagBio Genomics at (301) 302-0144.

[reportsanddata]

Epigenetics Market  Revenue Share, Key Growth Trends, Major Players, and Forecast, 2020–2028

According to the current analysis of Reports and Data, the global Epigenetics market was valued at USD 9,143.1 Million in 2020 and is expected to reach USD 36.52 Billion by 2028, at a CAGR of 18.8%. Epigenetics refers to changes in gene activity without altering the DNA sequence, the changes being transmittable to daughter cells. Epigenetic processes are natural and essential for the functioning of many organisms, but odd occurrences can prove to be a source of various disorders, including different kinds of cancers, reproductive illnesses, cardiovascular illnesses, etc. In addition to this, behavioral changes and changes in mental state are also observed on account of epigenetic changes.

Several illnesses and other health indicators may have direct or indirect link with epigenetic mechanisms, including various cancers, respiratory diseases, cardiovascular diseases, reproductive complications, autoimmune disorders, and cognitive dysfunction ailments. Agents like heavy metals, pesticides, tobacco smoke, hormones, radioactivity, viruses, environmental factors, and basic nutrients that contributes towards such conditions are also on rise, which supports the growth of the market.

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DNA Methylation, histone methylation, acetone methylation are among the key technologies used for epigenetic applications. Research activities have found strong links between DNA methylation and aging. Public organizations such as the International Human Epigenome Consortium, the National Cancer Institution (NCI), and the National Institute of Health (NIH) are influencing the market positively through financial support for the research & development in field of epigenetics. Moreover, the rising focus on study of epigenetic modifications at the genetic & molecular level and development of epigenetics based therapeutic solutions are likely to positively influence the funding scenario for the research programs. Furthermore, the Epidemiology and Genomics Research Program (EGRP) grants endowment to research-related activities and related need for personalization in healthcare owing to genetic variations, expanding application in non-oncology diseases, target therapy is anticipated to provide growth opportunity to the market players in the coming years. Several funding and R&D initiatives are undertaken by private institutions, and biopharmaceutical companies for the detection of a biomarker for drug development and disorder are driving the market growth. Also, the growth of the geriatric population in regions around the world is expected to endorse the growing market. However, the high initial cost and maintenance costs of the instruments prove to be a significant hindrance to market growth. Incidences like the shortage of trained professionals and concerns regarding the quality of antibodies are substantial challenges for the epigenetics market.

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The report includes descriptive profiles of major companies, which consists of the legal names, headquarters, website, market standing, historical development, and top 5 closest competitors derived by inspecting their gross revenue, along with contact information. Every company’s growth rate, gross revenue, and profit margin have also been discussed in the study. It also assesses other expansion tactics implemented by leading companies including mergers, acquisitions, and product launches.

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Further key findings from the report suggest

Reagents like antibodies, PCR reagents, histones, primers, magnetic beads, electrophoresis reagents, nucleic acid analysis reagents, and buffers are extensively used for epigenetic studies. This contribute to high market share of the reagents segment in the epigenetics market

The histone methylation segment is expected to reach USD 5.65 billion by the year 2026, showing significant growth of 13.1% during the forecast period

The emergence of epigenetic targets for the development of epigenetic drugs and epigenetic therapy is expected to have a highly positive impact on the epigenetics market. Leading market players across the globe are investing significantly towards product development for epigenetics based applications. For instance, in April 2019, GGC launched its EpiSign, a novel clinical test for analysis of epigenetic changes.

The European segment is expected to show a CAGR of 12.8% owing to increased investments in healthcare projects. For instance, project BLUEPRINT, which aims at generating 100 reference epigenomes of healthy and leukemic cells, received a funding of 52.1 million USD.

Market players are adapting various organic and inorganic expansion strategies. For instance, 10x Genomics, a key player in genomics field acquired Epigenomics, a market player operating in epigenetics sector and merged their epigenetic technology with its chromium single-cell solution.

Leading as well as emerging market players are involved in various research and development activities. For instance, an epigenetic biotechnology company - EpigenCare completed its initial pilot study in 2019, with its direct-to-consumer epigenetic skincare test as part of its beta phase

Key Companies in the market include:

Illumina, Thermo Fisher Scientific, Merck Millipore, Abcam, Active Motif, Bio-Rad Laboratories, among others that collectively constitute a competitive market

Market segment analysis:

Product Type Outlook (Revenue in USD Million; 2018–2028)

Chip sequencing kit

Whole Genomic Amplification kit

Bisulfite Conversion kit

RNA sequencing kit

Others

Technology Outlook (Revenue in USD Million; 2018–2028)

DNA Methylation

Histone Methylation

Histone Acetylation

Large non - coding RNA

MicroRNA modification

Chromatin structures

Application Outlook (Revenue in USD Million; 2018–2028)

Solid tumors                        

Liquid tumors

Inflammatory diseases

Metabolic diseases

Infectious diseases

Cardiovascular diseases

Others

End Use Outlook (Revenue in Million USD; 2018–2028)

Academic and Research Institutes

Pharmaceutical and Biotechnology Companies

Contract Research Organizations (CROs)

Regional analysis further covers a country-wise analysis to offer insights into key trends and demands in each major country that might affect the growth of the market in the region.

North America

Europe

Asia Pacific

Latin America

Middle East & Africa

US.

Canada

Mexico

Germany

UK.

Italy

France

BENELUX

Rest of Europe

China

India

Japan

South Korea

Rest of APAC

Brazil

Rest of LATAM

Saudi Arabia

A.E.

South Africa

Rest of MEA

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[afkebooks]

Cancer Biology and Treatment (Oxford Biology Primers) (Original PDF from Publisher) #Oncology #OxfordUniversityPress

[shavara1]

Radiation Oncology Billing Software

Oncology EMRs furthermore suggested as electronic prosperity records (EHRs), are planned to meet the novel necessities of oncologists. Regardless of anything else, this suggests managing the mentioning and association of chemotherapy drugs. These EMRs furthermore have intriguing sickness and hematology work cycles and designs and may have applications for uncovering, harm vault and clinical fundamentals. The Radiation Oncology Billing Software is the best robotizing dosing, mentioning and booking thwarts botches by simplifying requests to scrutinize, supervise and manage. Oncology EMRs should moreover combine the documentation for drug admixture and association, including stock organization for addressing any billable waste.

 Oncologists have the noteworthy yet unenviable charge of aiding their patients through quite possibly the most inconvenient events of life. Preparing is extremely valuable in leaving patients with the experience of compassion and support as they investigate this test. A fantastic Radiation Oncology Billing Software will allow the specialist to record the information given to the patient, so you can be sure they are left totally maintained. Features may fuse a database of stream research and advancing fundamentals and modernized calm primer capability planning, both to find existing starters for new patients and to bring existing patients into as of late supported primers.

[fostermarketarch]

Isothermal Nucleic Acid Amplification Technology Market Comprehensive Analysis And Vendor Landscape 2027

The global isothermal nucleic acid amplification technology market was valued at US$ xx million in the year 2019. This market is estimated to be valued at US$ xx million in the year 2020 and is expected to reach US$ xx million by the year 2025, with an estimated CAGR of xx% during the forecast period (2020-2025).

The increasing emergence of low cost, effective, and faster diagnostic procedures is driving market growth. Further, the growing aging population and growing different kinds of diseases are further propelling the isothermal nucleic acid amplification technology market growth.

Competitive Landscape:

Key players in the global isothermal nucleic acid amplification technology market include EIKEN CHEMICAL CO., LTD, QIAGEN, Dickinson & Company, Tecan Trading AG, Abbott Laboratories, Inc., GE Healthcare, Hologic, Thermo Fisher Scientific, Inc., Genomtec SA, Ustar Biotechnologies Ltd., and Sysmex Corporation among others.

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The isothermal nucleic acid amplification technology market players are focusing on several business growth strategies including merger, acquisition, new product launch development, etc. For instance, in May 2019 Hologic, Inc. launched Aptima BV and Aptima CV/TV assays for accurate diagnosis of vaginitis, a complex issue in women. Aptima consists of molecular nucleic acid amplification tests that detect candida vaginitis, bacterial vaginosis, and trichomoniasis.

Key Insights:

Latest Updates

Analyst Views

Future Outlook of the Market

Market Taxonomy:

By Technology

Nucleic Acid Sequence-Based Amplification (NASBA)

Loop-mediated isothermal amplification (LAMP)

Helicase-Dependent Amplification (HDA)

Strand Displacement Amplification (SDA)

Nicking Enzyme Amplification Reaction (NEAR)

Single-Primer Isothermal Amplification (SPIA)

Transcription-Mediated Amplification (TMA)

Recombinase Polymerase Amplification (RPA)

Rolling Circle Amplification (RCA)

Others

By Product Type

Instruments

Reagents

By Application

Infectious Disease Diagnostics

Blood Screening

Oncology

Others

By End User

Hospitals

Academic and Research Institutes

Diagnostic Laboratories

Others

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Market Dynamics:

Nucleic acid amplification is an essential technique from basic laboratory research to medical diagnostics. With improved cost and amplification techniques, the application of diagnostic nucleic acid amplification offers wider access and has extended outside the specific laboratory settings.  Loop-mediated isothermal amplification (LAMP) in one of the isothermal nucleic acid amplification technology which has been used in a point-of-care diagnostics. According to the WHO, around 17 million individuals are dying annually due to infectious diseases across the world. Around 30 types of new diseases have emerged in the last 20 years which risk the lives of millions of people. Isothermal nucleic acid amplification technology enables the diagnosis of diseases such as HIV-AIDS, trachomatis, pneumonia, etc.

The innovative isothermal nucleic acid amplification technology enables early detection of these infectious diseases, thus reflecting the various opportunities for growth in the coming years. For instance, in February 2019, Grifols, an innovative diagnostic solution development company introduced the Procleix Babesia assay which is approved by the U.S. FDA. Procleix Babesia assay used for finding the ribosomal RNA from 4 Babesia species; the assay operates on a fully automated platform using Nucleic Acid Testing (NAT) for blood screening.

FAQ's:

Note: This report provides an in-depth analysis of the global isothermal nucleic acid amplification technology market and provides market size (US$ Million) and compound annual growth rate (CAGR %) for the forecast period (2020-2025), considering 2019, as the base year.

What are the trends adopted by key players in the global isothermal nucleic acid amplification technology market?

What key factors are expected to increase the demand for the isothermal nucleic acid amplification technology market during the forecast period 2020-2025?

What are the major challenges inhibiting the growth of the global isothermal nucleic acid amplification technology market?

What is the total market value (US$ Mn) generated in the global isothermal nucleic acid amplification technology market by product type in 2019 and what are the forecasts by 2025?

Which application is expected to dominate the isothermal nucleic acid amplification technology market in the coming years?

What was the total revenue generated by the global isothermal nucleic acid amplification technology market across different regions (North America, Europe, Asia-Pacific, Latin America, and the Middle East and Africa) in 2019, along with CAGR (%) for the period (2020-2025)?

Which technology will foster the isothermal nucleic acid amplification technology market growth in the coming years?

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