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snarksandkisses · 5 years ago

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What I think about COVID-19 this morning - Malia Jones, PhD, MPH

What I think about COVID-19 this morning

March 5, 2020

Maybe I'm the closest thing you personally know to an infectious disease epidemiologist. Maybe not--I'm not an expert on this virus by any stretch, but I have general knowledge and training from studying epidemics that is applicable, so here are my thoughts.

First and foremost: we are going to see a tremendous increase in the number of US cases of COVID-19 in the next week. This is not because of some new pattern in the spread of the disease, but rather due to a major change in the requirements to be tested. Until yesterday, if you had flulike illness but had not recently traveled to China, Italy, South Korea, or Iran, you could not be tested. This is just the way healthcare works, you get tested if you meet the case definition and the case definition included travel.

As of yesterday, you can be tested if you are sick and have a doctor's order to be tested. So expect things to feel a lot more panicky all of a sudden. We will see hundreds or thousands of new cases as a result of testing increases.

Second: is that panic legitimate? Sort of. This is not the zombie apocalypse. The death rate of 30 deaths per 1000 cases is probably a wild overestimate. (The denominator is almost certainly wrong because it is confirmed cases--and we only confirm cases when we test for them). That said, even at 3 per 1000 cases, this would be a big deal. A very big deal. By way of comparison, the death rate for influenza is between 1 and 2 in 1000 cases. So, yeah. Roughly 0x to 30x worse than a huge global flu pandemic? That's a problem.

Unlike flu, COVID-19 is not *particularly* dangerous for children, so that’s some happy news. It is dangerous for older adults and those with lung conditions, so we need to be extra careful to protect those populations from exposure.

Also, for millions of Americans, getting any serious illness requiring a hospitalization is a major problem because they can't pay for it. And our health care system is probably going to struggle to keep up with it all. And with China basically closed, our global economy is going to take a huge hit and we'll feel the shockwaves for years. Those are real concerns.

What can we do? Our focus should be on *slowing down the spread* of this disease so that we have time to get caught up. Here is my advice:

1. Wash. Your. Hands. Wash them so much.

The current best guess is that coronavirus is transmitted via close contact and surface contamination. A very small study came out yesterday suggesting that the virus causing COVID-19 is *mostly* transmitted via contact with contaminated surfaces.

I have started washing my hands each time I enter a new building and after being in shared spaces (classrooms especially), in addition to the standard practice of washing after using the bathroom and before eating. Soap and water. Hand sanitizer also kills this virus, as does rubbing alcohol (the main ingredient in hand sanitizer).

There is no need to be obsessive about this. Just wash your hands. A little bit more effort here goes a long way.

2. Don’t pick your nose. Or put your fingers in your mouth, on your lips, or in your eyes. Surface contact works like this: you touch something dirty. Maybe it's an elevator button. Virus sticks to your hands. Then you rub your eye. Then you touch your sandwich, and put the sandwich in your mouth. Now there is virus in your eyes and mouth. See?

You may be thinking, but I don’t pick my nose because I am an adult! An observational study found that people sitting at a desk working touched their eyes, nose, or lips between 3 and 50 times per hour. Perfectly normal grown-ups, not lowlifes like my friends.

2a. There was one note that came out suggesting that face masks actually promote surface contamination because you're always adjusting them--i.e., touching your face. I don’t know if that’s true. But face masks should not be worn by the public right now, unless you are the person who is sick and you're on your way to or actually at the doctor's office. The mask’s function is to prevent spit from flying out of your mouth and landing on things when you cough or sneeze. It flies out of your mouth and is caught in the mask instead. If you are the person who is sick and not on the way to the doctor, go home. Let the people who really need them have the masks. Like doctors.

[ETA on 3/6/2020 honestly people I am getting so much push back on the mask recommendation!! The world is running low on masks. If everyone wants a mask so they can feel ok about keeping their Daytona Beach Spring Break plans and then hospitals in India can't buy them anymore, shame on us.]

Coronavirus does not appear to be airborne in the sense that doesn't remain floating around freely in the air for a long time, like measles does. You are probably not going to breathe it in, unless someone is coughing in front of you. If someone is coughing in your face, feel free to tell them to get their ass home and move 6 feet away from them. (Yeah I know, if you have a toddler, you're screwed.)

3. Sanitize the objects you and lots of other people touch, especially people outside your family--like door handles, shared keyboards at schools (brrr), salad bar tongs, etc. Best guesses are that the virus can live on surfaces for 2-48 hours, maybe even longer, depending on the surface, temperature, and humidity.

Many common household cleaning products will kill this virus. However, white vinegar solution does not. You can make your own inexpensive antimicrobial spray by mixing 1 part household bleach to 99 parts cold tap water. Spray this on surfaces and leave for 10-30 minutes. Note: this is bleach. It will ruin your sofa.

4. "Social distancing." You're going to get so sick of this phrase. This means keeping people apart from one another (preferably 6 feet apart, and sanitizing shared objects). This public health strategy is our next line of defense, and its implementation is what will lead to flights and events cancelled, borders closed, and schools closed.

For now, you could limit face-to-face meetings, especially large ones. Zoom is an excellent videoconferencing option. If you spend time in shared spaces, see #1. Ask your child's school about their hygiene plan, if they haven't already told you what it is. If I were in charge of a school setting, I'd be hand sanitizing the s*** out of the kids' hands, including in and out of each space, and taking temperatures at the door. I am planning to email our school nurse right after this to ask if they need my volunteer help cleaning surfaces.

If you can telecommute, do that a little more. If you are someone's boss and they could do their job remotely, encourage them to do that.

Avoid large gatherings of people if at all possible, especially if they are in an area with cases OR places that lots of people travel to. If you attend group events and start to feel even a little bit sick within 2 to 14 days, you need to self isolate immediately. Like for a tiny tickle in your throat.

5. All your travel plans are about to get screwed up. If you are considering booking flights right now, get refundable tickets. ETA: most trip insurance will not cover cancellations due to a pandemic. Look for "cancel for any reason" trip insurance.

Considerations for risks related to that trip you’re planning: how bad would it be if you got stuck where you are going for 3 to 6 weeks? How bad would it be to be isolated at home for 2-3 weeks upon your return? Do you have direct contact with people who are over 70 and/or have lung conditions? If those seem really bad to you, rethink your trip, especially if it is to a location where there are confirmed cases.

6. If you are sick, stay home. Please! For the love of all that is holy. Stay at home. Your contributions to the world are really just not that important.

7. There is a good chance some communities will see school cancelled and asked to limit non-essential movement. If someone in your family gets sick your family will almost certainly be isolated for 2-3 weeks (asked to stay at home). You could start stocking up with essentials for that scenario, but don't run out and buy a years' worth of toilet paper. Again, not the apocalypse. 2 weeks' worth of essential items. Refill any prescriptions, check your supply of coffee, kitty litter, and jigsaw puzzles.

8. I do want to remind everyone that when public health works, the result is the least newsworthy thing ever: nothing happens. If this all fizzles out and you start feeling like ‘Wah, all that fuss for nothing??’ Then send a thank-you note to your local department of public health for a job well done. Fingers crossed for that outcome.

9. Look, I think there are some positives here. All this handwashing could stop flu season in its tracks! We have an opportunity to reduce our global carbon footprint by telecommuting more, flying less, and understanding where our stuff comes from. We can use this to think about the problems with our healthcare system. We can use this to reflect on our positions of privilege and implicit biases. We can start greeting each other using jazz hands. I'm genuinely excited about those opportunities.

There is a lot we don't yet know about this virus. It didn't even exist 90 days ago. So stay tuned, it is an evolving situation. The WHO website has a decent FAQ. Free to email or text with questions, and you can forward this to others if you think it's useful.

May the force be with you.

Malia Jones, PhD, MPH

[email protected]

I’m an Assistant Scientist in Health Geography at the Applied Population Laboratory at the University of Wisconsin-Madison. I study social contact of humans, and spatial patterns of infectious disease, among other things.

P.S. The number one question I am getting is, did you really write this? Yes. I wrote this.

I didn't write it for professional purposes, so I didn't put my work email on it. It was really just meant to be an email to my friends and family in advance of what I expect to be an escalation in the panic level. But it was apparently welcome information and went viral on FB. I've decided not to edit out the swears, even though I wrote this with a much smaller audience in mind.

Thanks for checking your facts! Go science!

#COVID-19 #coronavirus #wash your hands #Social distancing #don't panic #but maybe just a little panic

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softandweto · 4 years ago

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Help

I know I should put this up somewhere else, but I can’t. Tumblr is my only option and I need all the help I can get right now please.

I had to make a GoFundMe because there’s nothing else I can do. Please spread the word and the link is right here. I’ll be putting the same info in the GoFundMe under the Read More for those who want information up front.

Hello, I hope this finds any who visit well. Before I get into the reason for me making this, I’d like for you all to know how we got here.

My name is Xenia and my boyfriend and I have been living together for nearly a year now. At the beginning, things were going very well. We both had a stable income, and while that trailer was not the best place, we were secure and didn’t have to worry much about finances. Then, March came around and Covid hit us hard. I lost my job as a Teaching Assistant for Special Education, and my boyfriend’s job got more dangerous as a Med Tech working in a nursing home. I was unable to find a job for months, and things were starting to take a bad turn.

We had some friends living in the state next over who had offered the both of us to move in with them. My boyfriend would have to go back to CNA work, however, as that would be the only license of his that could transfer to the state. My license as a Teaching Assistant was originally for that state, so it seemed to be perfect. After weighing all our options, we decided to make the move and take the risk.

Once again, things were looking well. We both got a new job within the month, and only had to worry about paying a combined $600/month outside of our car payments. With all this, we were able to put up with a lot of things. A majority of the house leaving the place in disarray for the both of us to handle. The racism that we didn’t catch up on until the end. The disregard for my dogs and their health. The fact that, despite how behind the house supposedly was on bills, they could afford to continue to finance new furniture and electronics while we could barely afford to pay the rent and our own food. We could put up with it because we were with friends. No way they would do all this on purpose.

Eventually, after two months of living there, it became too much and they used every excuse possible to force us out of their home and ostracized us. Suddenly, we were the issue. It was our fault their dishes continued to pile up. It was our fault they felt too anxious to leave their rooms. All their problems were now because of us. We had no other choice to move in with my mom and my brother in our old state. Once again, we were out of jobs and couldn’t find work no matter where we looked. I eventually found a job as a server again, but he was unable to find any work despite his CNA credentials.

When October came around, I was working full time for a server minimum wage, while my boyfriend had finally gotten some good news and was starting to work. We scrimped and saved for two months and were finally able to get enough to get our own home. A trailer in a small suburb just outside town was freeing up early December. At first, the price for the rent seemed impossible to make. But, I had received an email from a work from home position I applied for. Early January, I would be starting with them for more than minimum wage.

Things were finally coming into place. Things were once again looking up and we could taste the stability. Then, after a week of being moved in, we decided to enjoy a meal together made in our own home. All the stress, all the craziness we had put up with, it was worth it. But, we couldn’t taste our food. We started noticing the coughs when we were moving, but didn’t think much of it till then. We got tested, and our fears proved to be true. We had Covid.

It was brutal. It felt like suddenly we’d lose everything. The two weeks we spent in quarantine was like our own personal hell scape. Within the first week I was notified they were training someone else to take over my Shift Lead position. A title more than anything, since the pay did not change and minimum wage was all I could get, but that didn’t stop what I knew was coming next. A few days later, I was let go. Tossed aside like an inconvenience. For my boyfriend, they just put him out entirely. For the third time in one year, we were both out of a job. But now, we could face eviction.

We recovered from Covid, and just in time too. I was able to start my new job, but two weeks of no pay had put us out tremendously. One company hired my boyfriend, but we would shortly learn that they would never actually give him any hours. December and January have tested us on what we could and couldn’t live without. We had to forgo a majority of necessities.

We couldn’t set up a disposal service. We had to leave mail to pile up. Living off Dollar Tree groceries. Go weeks without gas. Pawn what we could just so we could make rent and utilities. Now, with February ending, all of this has caught up to us.

Months of garbage have piled up so high we’ve designated a “trash room” just to keep it out of the way. Toiletries have been out for weeks, but we can’t even afford groceries so soap and cleaning products are out of the question. Our propane is almost completely gone. All the cans of food we had stockpiled are a day away from running out. And we can’t afford our bills. Not with all my checks being used to barely keep us alive.

My boyfriend has recently started a new job, but they won’t pay him in time for us to pay our bills. Which is why I’m reaching out to y’all for help. We have both done everything in our power to keep ourselves above water, but now we can no longer keep it up on our own.

Here is a breakdown of our situation as of today:

My recent paycheck is completely gone after using it to get some of our bills stabilized, but they are already getting back into the red with how far behind we are.

Our car payments are coming up as well as insurances. One car payment is my full check, and we won’t be able to pay for one of them, much less their insurance

We were able to get rid of four bags of trash thanks to some helpful neighbors, but it’s starting to pile once more and I’m worried bugs will start to come out

Internet and Electric must be paid within the next few days in full or risk disconnection. With these two gone, I can’t make any money whatsoever

Food will be out as of Tuesday and with no money left from my check, we’ll be unable to get any groceries for who knows how long

We just ran out of Propane which is used to keep water hot as well as to cook

I hate asking for help and not letting people know what the situation is or what the money will be used for, so I will do so now.

I am asking for 2500 which will leave us with a touch of extra money for things like groceries, toiletries, and vehicle maintenance that is greatly needed. The breakdown is as follows:

$550 - Rent

Rent is due on the 12th of each month and requires two checks to meet. Last month we were able to pay in two separate payments, but our landlord has said that it was the only time and March forward it will need to be in full each month.

$650 - Car Payments

Both cars are $300/month, but we’ve passed my boyfriend’s due date and have incurred a late fee. My car is due on the 6th and if it’s not paid in time, they will repo.

$500 - Insurance

Both Insurances are ~$250 each. Without the insurance, the cars will also risk repossession and my boyfriend needs the vehicles for transportation

$235 - Internet + Electric

I’ve lumped these together since they are both necessary for my job as well as being ones that need to be paid by this Tuesday or they will disconnect

$100 - Propane

$100 gives us enough propane to last a month. Without this, we can’t shower, do laundry, or even cook

$120 - Disposal + Mail

Disposal and Mail service needs to be set up as soon as possible, but to be honest they are low on my priority list compared to everything above.

$345 - Groceries, Toiletries, Cat Care, and Car Maintenance

With the extra money we can comfortably get through a month with little hassle. I know that more bills will be due later on, but once my boyfriend starts getting steady checks again We can at least make it through on our own with this little extra

I know that right now, things are very tough. I may also come about as rather...presumptuous and hopeful that maybe, just maybe, people can help us out in our time of need. I know it’s a lot to ask, but if you see this and are unable to help us out yourselves, please spread the word as much as you can. I cannot allow us to fall after everything we’ve been able to get through this horrible year. Please, if you can give even a dollar, that’s one dollar closer to getting out of this hole.

Thank you, and I hope that you all have a safe and happy time going forward.

#gofundme #help #homeless #bills #covid #im sorry yall im just at my limit and getting desperate

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addierose444 · 4 years ago

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A Typical Week: Spring 2021

Before getting into the actual content, I thought I would just point out that this is my 100th blog post! You can check out the full list here.

As noted in my fall 2020 version of this post, my week is primarily dictated by my current course schedule. (To check out all of my past courses, click here). Furthermore, the way I write these posts is to focus on academics as they're a big part of my life, but also the most natural thing to write about publicly. This post should give you a realistic sense of the structure of my week and courses. I thought it may be useful to contrast expectations and reality when it comes to productivity. Early in the semester, I mapped out my idealized homework schedule around my meetings and assignment deadlines. The first row lists deadlines. The other rows are split by my meals (lunch and dinner). Even if I don’t strictly follow the schedule, spending the time to map things out is quite useful. To write about my real schedule, I actually looked back through data from my time tracking application, Toggl Track. I know this is a very long post, but it wasn’t really interesting enough to become a two-parter.

A typical school day starts off with my 9:00 (or 9:10) alarm. I also often have a second alarm set for 9:15 as a backup. Here at college, I simply use the default clock app on my phone and have it play from my favorites playlist on Spotify. I very occasionally need to wake up earlier to finish up an assignment as midnight is my strict cutoff for doing work. I know a consistent sleep schedule is very important, but it’s definitely something I struggle with.

Monday:

My week begins with a 9:20 math class. The specific course is math methods which as previously explained is an applied math course from the physics department that is required for physics and engineering majors. We usually start off by going over the pre-class check in (PCCI) and/or other questions students have. This review is followed by a lecture on new material. Throughout the class, we work through example problems in breakout rooms (on Zoom whiteboards) and answer multiple-choice questions using the poll feature. (The poll questions are anonymous and ungraded). Partway through the class, we get a 4-minute break. One nice thing about this class is that we actually thoroughly go through the example problems when we regroup as an entire class. This is important because, without feedback, practice is of limited utility. Furthermore, going over the problem gives me a chance to get everything into my notes. The integration of lectures with practice is something I really appreciate about this course. In past math classes, the format has been a lecture followed by a worksheet of practice problems. While that model sometimes makes sense, I much prefer this integrated approach. One issue with leaving practice until the end is that you sometimes run out of time and don’t catch knowledge gaps until the end of class. After math methods, I get a 20-minute break during which I often listen to the latest episode of The Daily (a short new podcast from the New York Times).

Next up is my philosophy of logic class which starts with a few minutes of breathing and stretching. On the first day of class, I thought this was a really strange thing, but have come to appreciate it. Afterward, we go over any relevant announcements and sometimes debrief the previous class. This class is different from my other classes in that it is reading and discussion-based. We spend most of the class in the main room strengthening our understanding of the reading through full class discussions and mini-lectures. Even though the class is already very small (13 students), we also make use of breakout rooms to work through study questions. Our tests are pyramid style which means we spend one class period working independently and another class on the same questions in a small group. We also have short quizzes, called mini check-ins, every few weeks. Next up is lunch during which I sometimes listen to a podcast or audiobook. You can check out some of my favorite podcasts and books of 2020 at the associated links.

After lunch, I have my computer systems lab. The teaching assistant of my lab section starts off with a quick introduction to the assignment. We then work independently and ask questions as they come up. Even though we don’t really work collaboratively the lab is sort of fun because it’s less formal than a regular class. For the first part of the semester, the lab assignments often took way longer than the allotted time (and sometimes longer than our projects) so I often spent more of the afternoon finishing up the assignment. Fortunately, the course staff was made aware of this issue and adjusted accordingly. Just for some context, this course is a UMass class which is why there is a whole course team and teaching assistants. To read more about Five College course registration click here. At Smith, while some classes have teaching assistants who help out during class, all of the classes are taught and run by our actual professors. We also have student tutors as an academic resource outside of class. To read more about academic support systems at Smith, click here.

After finishing up my lab assignment, I start in on my math methods PCCI. A typical PCCI consists of reading a section or two of the textbook (written by our professor) and completing a short practice problem or so-called discovery exercise. Depending on the week, I either start in on my reading assignment for my computer systems class or logic class. At 4:00, I have my weekly one-on-one meeting with my supervisor for my ResLife job. Following the meeting, I relax by listening to music or an audiobook. At 5:00, I order, pick up, and eat my dinner. After dinner, I complete any remaining readings for my computer systems and logic classes. If I haven’t yet completed my lab assignment or have an exam the following day, I devote some of the night to circuits. Otherwise, I may work on a computer systems project or theory of computation assignment.

Tuesday:

Tuesday’s are my busiest day of the week in terms of class hours. With that said, it’s nothing compared to my Thursdays last fall. I start Tuesdays off with my circuits class. During class, we learn new circuit theory and circuit analysis techniques. We also go through example problems. While we often run out of time to fully work through the extra practice problems in breakout rooms, fortunately, my professor posts videos going over those problems. After taking our feedback into account, we now get a break partway through the class. Each lecture covers a lot of material, so the brain/screen break is much appreciated. To check in on how the class is feeling about various concepts, our professor has us use the annotation feature on a scale from totally get it to totally lost.

After circuits is my 20-minute break followed by my theory of computation lecture. The theoretical nature of the material means that it really is a lecture. While we obviously go through examples, there aren’t really practice problems as there would be in a math class. We use the chat to some extent in all of my classes, but to a greater degree in this course. Next up is lunch and a COVID test. At 1:40 I am back to circuits for the lab. Most of the labs are virtual with physical lab equipment, but a few have been in-person so that we could use special equipment. Ironically, one of our in-person labs was actually fully virtual in terms of lab equipment. (We were sitting on the lawn outside of Ford together and running circuit simulators on our laptops). Fortunately, we don’t usually need to stay until 4:30. I tend to just collect my data, ask some questions about the material, and then finish up the write-up at a later point in time. This time block is also the one used whenever we have an exam.

I always start by doing the textbook reading for circuits. I don’t read super closely, but it’s still a good primer for the next class. In terms of other work, Tuesdays are a bit more unpredictable and really depend on how much I got done over the weekend. Specifically, while I usually finish my math methods assignment over the weekend, occasionally I need to finish it up on Tuesday evenings. Likewise, for circuits, I sometimes finish the last few problems on a Tuesday evening. At 7:00, I have a staff meeting with the other community advisors in my neighborhood and our supervisor. Our meetings usually take place over Zoom, but our most recent one was in-person with ice cream from Herrell’s which was a lot of fun. You can read about some other Northampton food locations (restaurants, cafes, and more) here. In weeks where I haven’t yet started my computer systems work, I do what I would usually have done on a Monday on a Tuesday.

Wednesday:

Wednesdays are similar to Mondays without the computer systems lab and ResLife meeting. In the afternoon I often attend office hours for my theory of computation class to ask questions about the weekly assignment. Even though I don’t have my logic class until the following Monday, I usually just do the reading on Wednesday afternoon. On Wednesday evenings, I typically work through my circuits revisions. I also tend to do a good chunk of my computer systems coursework. This consists of watching lecture videos, taking notes, and taking lesson quizzes. Furthermore, I have definitely spent some Wednesday evenings working through computer systems projects.

Thursday:

Thursdays are like Tuesdays without the circuits lab. Even though I have the whole afternoon free, unfortunately, I am sometimes having to finish up my theory of computation assignment. It’s also common for me to start working on the new math methods problem set. In the case of this Thursday, I played some guitar and then started writing this post. I also do my circuits reading for Tuesday and take the quick lab quiz. If I have any remaining computer systems coursework, I do that as well.

Friday:

This semester I only have one class on Fridays, math methods. After class, I get a COVID test and an early lunch. I know it seems crazy how many free afternoons I have given that I am taking 22 credits and am a double STEM major. However, part of this is explained by my UMass computer systems course being asynchronous and the fact that I completed the one-credit companion course in C programming before my Smith semester started. My computer systems class was originally scheduled to meet Monday, Wednesday, and Friday afternoons. Even though I wish the class was synchronous, the flexibility of an asynchronous class has been much appreciated. Furthermore, the class was in a terrible time block that would have caused me to miss most of house tea. Back to what a Friday afternoon looks like! After completing my PCCI for math methods, I often rewatch the lecture videos for computer systems (on double speed) and then take the weekly quiz. I next pick up tea snacks from Cutter-Z and attend house tea at 4:30. After tea, I order dinner and often eat it in the living room with housemates. Fridays are definitely my least productive day and I have definitely taken a few weekly quizzes on Saturday after having planned to take them on Friday. Instead of doing real work, I often spend Friday afternoons writing blog posts. As for this post, I wrote most of it yesterday but spent a good chunk of Friday afternoon on it as well.

Saturday:

Despite my best efforts to have a consistent sleep schedule, I often sleep in on Saturdays until 10:00. I then have a leisurely hour or so of listening to an audiobook. At 11:00, I get an early lunch. As mentioned in the Friday description, I sometimes end up taking my weekly quiz for computer systems on Saturday. When I have tests in math methods, I typically take them on Saturday night. (The tests are timed but are self-scheduled over the given weekend which includes Friday). When there is not a math test, I often work on my math problem set in the afternoon. Every few weeks, I host POCheese at 4:00. This week we are actually going to be meeting at 5:00 for a ramen night! At 6:00 I have a uke club meeting over Zoom. In weeks where I have already finished my math problem set and don’t have a test, I start in on my circuits problem set.

Sunday:

Sundays start like Saturdays in that I often have a leisurely morning. At 11:00 I get a COVID test and an early lunch. Sundays are almost always devoted to my circuits. This includes working through the problems set, the lab writeup, or studying for an upcoming exam. If my Saturday wasn’t as productive as intended, I do the homework described in that section. At 6:00 I have a Society of Women Engineers (SWE) board meeting. At 7:00, I either lead a community meeting (part of my ResLife job) or attend house council. Afterward, I fill out my weekly report (also part of my ResLife job).

#college #Smith College #academics #schedule #week in my life #homework #spring 2021

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loquaciousquark · 4 years ago

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I’m not very good at keeping up with life updates. I don’t know why! I never have been!

Carol moved out August 9th, about a week extra over the original two months we’d agreed on. She got an apartment about five minutes away, which is about 40 minutes from her job. I recommended getting an apartment closer to her job to ease the commute, but she said she strongly preferred being near to her friends and the city center. (She also had the option of a $650/mo lease for a year or $1100/mo for a six month lease at this complex, and opted for the shorter lease even though she couldn’t afford the $1100. She also insisted on a two bed 1.5 bath apartment instead of a much cheaper one bed one bath, and I don’t know why. Her financial choices continue to bewilder me.)

I helped her move, because she has a number of physical disabilities and a fairly total inability to accurately predict the scope of a job like this. She insisted to me multiple times that all she was picking up from her estranged mother’s home was a few books, a desk, a chair, and a small round end table. She came back with a fifteen foot Uhaul packed top to bottom with dozens of pieces of (often very heavy) furniture, tubs upon tubs upon tubs of books (not small boxes, like 40 gallon tubs immobile with books) and a lot of...I don’t know how to describe it.

Honestly, it reminded me of a hoarder’s home from those TLC shows. She had so much stuff, and almost all of it was...garbage. Like, stained, discolored, moldy, dirty, dusty furniture and blankets and clothes that were covered in mouse droppings and bird crap. An ancient armchair that she called “antique” but was made of very cheap very damaged veneered pressed wood and whose upholstery was of an indeterminate color because of how stained it was. A “display piece” of an old suitcase she picked up in some thrift store that was locked shut with what felt like twenty pounds of bricks inside, but the outside wasn’t even attractive - just an ugly brown box with no care or craftwork that was scratched and torn and stained with mouse poop. It honestly made me uneasy to touch it, and she was putting it in her apartment to use.

I don’t know how she thought she was going to be able to get all of this stuff down the half-flight of stairs to her apartment by herself. I helped her unload for four hours before I had another obligation, then came back after that finished for another hour or two of unloading to get it in her apartment securely. It was...very tiring, but also very exhilarating to be physically closing that chapter as well as mentally. I don’t intend to do it again.

My job (professor) started back in person on a regular basis the following week in August. We’ve now been in class for almost two months and no students that I directly interact with (my 46ish 2nd year students) have had any exposures or at risk scenarios. A small handful of 3rd and 4th years have had exposures, and I think one student actually tested positive, but none of the exposures or positive results were from our clinic or labs; they were all from community transmission (wife/husband/child/roommate caught it from somewhere else, student had to quarantine).

I have been almost draconian with my 2nd years about the thin line we’re walking in being allowed to have even our minimal, limited in-person labs, and have warned them that if we have an exposure breach in our clinic, there is zero chance they graduate on time in a few years. They seem to be taking it extremely seriously, which I like a lot. All students, staff, and faculty are given new surgical masks daily, and everyone who interacts with patients gets a new n95 mask every 10 days. We have decontamination stations throughout the building, temperature guns, digital “passports” that they have to update daily to be allowed on campus, and plenty of spray bottles with high level disinfectant for surfaces and hands. It’s honestly as safe as we can make it, and I feel we’ve hit a good balance between staying safe and getting them actually trained on real human beings.

That said, we are having more students than I’ve ever had fail exams and practicals across the board, and I honestly think it’s an artifact of the remote lectures (all lectures are remote unless specified for particular reasons, and then they only take place in distanceable classrooms, which are at max like 70% of the class). God knows I wouldn’t have learned as well at home - I’d have been on my phone or playing games and kind of half listening in the background, and if nothing else this has made me more of a proponent of mandatory attendance once it’s safe again to do so, because the drop I’m seeing is almost precipitous. Either this class is unusually full of students incapable of completing the program, or COVID’s striking again. I’m pretty sure it’s the latter.

On the other hand, we’re FINALLY making up the Injections course material that got stopped dead in spring, and the first run of it went very well last Saturday. I unequivocally resent that I have to give up an entire Saturday to do so (and will have to do so thrice more to get all the students done this semester), but it’s at least one chunk of incompletes that are now passes (and in fact, mostly As).

We just got the notification that hybrid courses will continue through spring. It’s so exhausting. We can do it safely, and we are, but it’s so hard.

Went to get a drink last night before bed and noticed the tea was a little warm. Went to grab some ice cubes from the freezer and discovered the ice cube tray was full of water, and the chocolate ice cream in the door had melted and spread across the entire unit.

Managed to get a repair guy out this afternoon, who charged $228 to replace the ...relay overload array capacitor, or something. He showed me what it was--a little black and white box that he said was bottom of the barrel cheap from China, which I fully believe, and installed a new one then and there while I had a remote test review with a student over Zoom. I put on headphones for her privacy, but she cried several times. It’s usually a pretty rough awakening when students who never had to study in college realize how difficult this program is and the study time required to pass, let alone succeed.

Anyway, the freezer’s chucking out cold air like it was made for it, and the fridge seems to be slowly working its way back down. Had to throw out everything from the freezer (chicken, steak, ground beef, bacon, veggies, frozen meals--and some pizza rolls) and I’m not looking forward to the grocery bill it’ll take to restock the fridge either, but at least it seems to be functioning again.

I just checked; the freezer’s made six ice cubes in the last three hours. I’ve put them all in my tea to celebrate.

Edit: just checked again and the fridge is slowly cooling off, thank goodness! Of course, I somehow managed to fall down the four stairs of the hall between here and there and bruise the royal bejeezus out of both hips and somehow the inside of my left foot, so I’m ordering in for dinner, because nuts to that.

#quark rambles #carol #the final update #one hopes #i don't know how to tag this

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raisingsupergirl · 4 years ago

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My Life With COVID-19: Week 1--Say Goodbye to Food

I never thought being a statistic would come with so much baggage. It's not that I thought it would never happen to me. In fact, I thought it already had happened to me. A couple of times. And maybe it did. But none of them were like this. I'm going to try to explain it as best as I can (you know, for science and future generations), but bear with me. COVID brain is definitely a thing.

I guess this story starts on 12/12/20. That's the day that my dear friend passed away. We were supposed to start a Dungeons & Dragons campaign together soon. Him, me, and three other good friends. But that Saturday, I got the text that he had passed away the previous night (not related to COVID, as far as I'm aware). Well, that following evening, those three other friends and I got together to remember him, to process some emotions, and to drink whiskey. The next day I woke up feeling… less than perfect. Of course, I thought it was from lack of sleep and too much alcohol, but it was weird. I didn't drink that much. Not to feel that bad. And there were some weird things, too. My eyeballs hurt (really bad) like I had a fever, but I didn't have a fever. And my fatigue level was through the roof. Other than that, normal body aches and lack of appetite that come with over-indulgence, so I didn't think much of it. Even when I woke up on Monday with persistent symptoms, I just assumed I was getting REALLY old and should never drink again. Yeah, I'm kind of dumb sometimes.

Monday and Tuesday could be characterized by general lack of energy, some mild congestion, and those danged achy eyeballs. And the only food that appealed to me was soup, and only in small amounts. "Just a little cold," I told myself. Even still, I had the good sense to be extra-cautious with my hand washing and mask wearing procedures. Unfortunately, I didn't have the good sense to get tested at that time. Mostly because my insurance doesn't cover testing (which is $150/swab), but also because I was in denial. I needed to work. My patients needed treatment. I was important… irreplaceable. And, of course, I didn't want to have to call my friends and tell them I'd exposed them Saturday night.

Wednesday was more of the same, but I felt even more fatigued. Then, someone else I'd come into contact with the previous week let me know that they'd tested positive. Crap. That's when the pieces started falling into place. And the last one fell as I was drinking a glass of alcohol (elderberry tincture, actually. Which I'd made myself as a COVID preventative… guess I should have started drinking it earlier…). While I sipped, I was actually hanging out with those same Saturday friends, but this time virtually. We were playing computer games. And about halfway through the glass of elderberry goodnes, I noticed that it wasn't nearly as floral or alcoholic tasting as it should have been. I assumed it was getting watered down, but suspicion started creeping up my spine. And by the end of the glass, it tasted like straight water (which tastes like nothing…). Like some infected dummy straight out of a zombie movie, I told no one and went to bed, hoping against hope that I would wake up to the smell of bacon (or anything).

When my alarm went off the next morning, I popped out of bed and shuffled to the bathroom. First thing I did? Took a long whiff of my deodorant stick. Nothing. I stuck the toothpaste up to my nose. Still nothing. Brushed my teeth. Foamy nothing. Went to the bathroom. Thankfully nothing. And then it was time to go downstairs, face my wife, and finally say it out loud. "I can't smell anything. It's completely gone." And that's the moment that it became real. No turning back. One rapid test later, and my fate was sealed. My boss started clearing my schedule for the next week, and my mind started racing with all of the people I needed to call. All the things I needed to do. What my life would look like for the next ten days. Even now, I don't know if the virus was effecting my cognition and emotions or not, but I do know that I was a mess.

By the time I got home, my world was spinning. I was angry, ashamed, confused, defeated, and overwhelmed. Mostly overwhelmed. I made sure my wife had pulled our daughter from school, and then I went up to my room. Not because I was quarantining from them, but because I couldn't handle being around anyone, even those whom I loved most (I mean, I'd be spending plenty of time with them over the next week anyway, right?).

Over the next hour, I felt like someone with an STD contacting all of my past… well, you know. I texted, I messaged, I called. Everyone was incredibly understanding. They all wanted to know how I was doing. And it felt almost shameful saying that I felt fine. "Just a little fatigued, eyeballs hurt a little, some congestion. And the no smell thing." It's funny how that didn't dawn on me yet. In the flurry of confusion, I hadn't stopped to consider what life without smell would be like. That revelation would come later. No, right now I was focused on the bigger things. I wouldn't be able to attend my friend's funeral this Saturday. I wouldn't be able to host Portmas (an annual Christmas celebration with those same friends) that night. I wouldn't be able to go to work for over a week. The days would feel like months… Have I mentioned that I'm a bit of a work-a-holic? Yeah, well, there was a BIG part of me right then that thought, "God did this. I wouldn't slow down. I wouldn't quit working. Even when I was sick, I was too dumb to take a step back. So God took my smell away. It's my fault for being so stubborn. And God finally stepped in." Yeah, those are some thoughts that I'll continue to unpack over the next couple of weeks, but for now it's enough to say that my thoughts and emotions were about as confused as my senses.

Speaking of which, my lovely wife made me a can of chicken-n-noodle soup for lunch. And it felt great. Warm, soothing, and satisfying. But with each bite, reality settled in the pit of my broth-laden stomach. It wasn't that I couldn't taste ANYTHING. There was something there. A touch of saltiness and a hint of umami (look it up). My tongue wasn't completely dead… but my nose was. And so, another cascade of confused emotions. More anger. More fear. Google said "most" patients got their smell back in a week or two, but for some it could take up to a year. And a small percentage never got it back. NEVER!? And at best, I didn't know if I could handle two weeks. Honestly, I didn't.

If you haven't lost your sense of smell, I'm sure you think I was overreacting. I would have, too, before it happened to me (Yes, I'm aware of the irony of my blog post a couple of weeks ago). But I want to try and explain the seriousness of this situation to you. Maybe fore some it's not so bad—those who are suffering REAL COVID symptoms. Those fighting for breath and for life. But for those of use who feel otherwise "normal," it's a panic-inducing affliction. For example, I'm a fledgling home brewer. Do you know what all beer tastes like when you have no smell? Like water with a ghost of bitterness on both sides of the tongue. Do you know what straight whiskey tastes like? Exactly the same with just a slight warmth in the chest. And so, my brewing hobby is done. Just done. And cooking? There's no point. Everything might as well be raw cucumbers and unseasoned French fries. Texture and temperature. That's literally the only variation. Well… almost literally.

In my panic, I NEEDED to know what my limits were. I needed to know if I could find any enjoyment from food. And so, I went to the extremes. Cloves, even when eaten straight, had absolutely no flavor. Straight salt registered a little on the tip and back of my tongue. Sugar felt kind of thick on my tongue, and if I tried imagining it, I thought I could taste it a little. Cayenne pepper was a little tingly in the back of my throat, but nothing more. Horseradish did nothing at first and only a little tingling on the top of my mouth afterward (mind you, I ate enough of all of these things to kill an elephant). And finally, I took a bite of a lime. Whoa! That about knocked me over. Imagine not tasting anything for 24 hours and then suddenly biting into a lime. That's exactly what it tasted like. Okay, well, I couldn't actually taste any lime characteristics, but that SOUR sensation registered off the charts. The sensation was both hopeful and frustrating, and those two emotions fit in perfectly with my general disposition.

That night, I was mean. Cranky toward my wife. She made dinner, and I was bitter about it. Airfried shrimp and tater tots with cucumbers on the side. She was TRYING to satisfy my texture and temperature requirements. And she did well. But it was still ash in my mouth, cotton balls in my stomach. And no one seemed to understand my frustration and fear.

But that night, I realized there was something I hadn't considered, too. My family is close. We hug and kiss. We cuddle. And so, there didn't seem to be any reason for me to start quarantining from them now. Besides, both of my daughters already had the sniffles, so the likelihood was high that they already had the virus. And my wife thought that she'd already had it a few weeks before. But… if she hadn't. If she was still susceptible. I wasn't worried about her safety, so much. She's healthy. She works out, eats right, and nurtures her already strong immune system. But, if she lost her smell, too…

Okay, hear me out. This isn't just about food enjoyment or fart detection (yes, my wife giggled at the dinner table because she farted right next to me without me knowing…). It's about safety. Have you ever considered how dangerous it would be to live in a house with a gas stove if no one could smell? I mean, presumably the kids might notice something, but would they understand enough to let us know? I'm honestly not sure I would take that gamble. So here's hoping my wife keeps smelling, because I really don't want to move out.

Oh, speaking of my wife smelling, there's one last revelation I had about anosmia (lack of smell). For an anosmic person to take a shower is truly a selfless act. Think about it.

Anyway, by the time I post this (12/23/20), my quarantine will officially be over. I will have spent a week at home. So I'll definitely have more to tell. But these first few days are enough for now. Stay safe, friends. And don't forget to stop and smell the hot cocoa before you miss your chance.

#covid-19 #anosmia #no smell no taste #covid symptoms #life with covid #quarantine #momblr #dadblr #mumblr

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webandmobileapplication · 3 years ago

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Benefits of Regression Testing in Software Testing & Quality Assurance

The COVID-19 pandemic has transformed the old world order. With the world reeling under the aftermath of this global pandemic and trying to get things back to normal as we know it. While customers were demanding before, the pandemic has only accelerated their quest for perfection.

Competition has never been tougher, but the right businesses have been encouraged by competition to go that extra mile by providing products or services that deliver something ‘more’. To ensure that all extra attempts don’t go in vain, software quality assurance plays a huge role.

The added features might be big or small, but they affect the overall functionality in some way which is obvious. Consequently, it is critical to test the existing features and make sure that the newer integrations don’t disturb what is already good enough.

This method is what we refer to as Regression Testing in software testing and quality assurance. If we go by a definition per se, regression testing refers to retesting the legacy changes to ensure that it functions with the same efficiency in a present, changed ecosystem too.

It’s a very handy method for QA testers to be knowledgeable about to better deliver their software testing and quality assurance services with efficient results.

Benefits of Regression Testing

When compared to the manual testing methods, regression tools are quicker, safer, and more accurate, making the lives of testers free of unnecessary hassle.

Here’s a look at some of the benefits of regression testing-

1- More Testing in Less Time-

There is a considerable amount of time required for the full coverage of the entire software. On the contrary, regression testing brings a few selected scenarios into consideration and then tests to check the software’s functioning.

To add to it, inputs can be tweaked at any point during the testing phase.

2- Top-Notch Software Quality-

The virtue of system stability is something that comes as a parcel with regression testing. To be truthful, it’s a big reason why regression has become a pivotal part of software testing and quality assurance services.

Shorter development cycles and top-notch software quality, become a reality.

3- Early Identification of Potential Bugs-

Proper regression testing provides a great idea about the bugs that are proving to be problematic after making changes to the software. In such cases, application codes can be modified swiftly without too much fuss.

Interestingly, the code value never gets compromised even after the change.

4- Upgrades to Existing Software-

Regression testing makes it easy to keep the software technology always updated with regular upgrades. It’s a proven method to keep the software relevant for extended periods of time. The outcome is a software product that’s immensely high on ROI and less time-consuming to a huge degree as every single step in the testing is fully automated.

There’s nothing that keeps the overall software testing and quality assurance on track other than regression testing.

Finally

Dedication and focus to regression testing will always be a sure step to make sure that the software is bug-free and flawless when it comes to the market.

The QA experts at Consagous Technologies are leading software quality assurance service providers and can help you out with a perfect solution to every requirement you have!

Original Source:

bit.ly/3dB8ev2

#softwarequalityassurance #software quality assurance service providers #software testing and quality assurance #software testing and quality assurance services

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thesevenseraphs · 5 years ago

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Bungie Weekly Update - 4/9/2020

This week at Bungie, the final bunker opened.

A third and final Seraph Bunker has opened on Io. The new Bunker points players to partake in the Seraph Tower event and new Legendary Lost Sectors on the Jovian moon. Guardians also made a new discovery in the bunker about Rasputin’s efforts to track our enemies. We’re one month into Season of the Worthy and we’re not done yet. Grandmaster Ordeal is only a few weeks away, but before we share more on that, let’s talk about a new charity initiative coming up next week.

MAKING A DIFFERENCE

As the world responds to the outbreak of COVID-19, many of us have been urged by our local governments to stay home. Containing the spread of the virus is one of the best things we can do to protect our fellow humans. If you’re a gamer, you’ve been training for this moment for your whole life. If you possess the heart of a Guardian, however, you might be wrestling with the urge to do more! This is the question we’ve been asking ourselves at Bungie:

Is there something more we can do to help the real-world heroes who are confronting this crisis head on?

We’ve arrived at an answer that we think is very exciting. We’d like to share it with you – and even welcome you to join us in helping to make the world a better place. On Monday, you’re invited to join us in our game rooms to talk about it.

Bungie’s COVID-19 Fundraiser

www.twitch.tv/bungie

Monday, April 13

10:00 AM Pacific

Players of Bungie games have never backed down from a fight. We’ve banded together to support the survivors of floods, earthquakes, and wildfires. You may even have a memento to commemorate the role you played in some of those initiatives. On this occasion, when it seems like the world needs help more than ever, we have an emblem to mark the Guardians in our community who choose to throw their lot in with our efforts.

On Monday, we’ll reveal how you can earn this emblem to display proudly in game. We’ll talk about this charitable call to action, who will benefit, and the impact that we will have together. Join us for the conversation. Join us to make a difference.

GRANDMASTER

In a few weeks, we’ll debut a new difficulty selection for The Ordeal called Grandmaster. It will test your fireteam’s coordination, communication, builds, and skill like no other activity in Destiny to date.   Here’s a look at a few of the goals we had when approaching Grandmaster after we saw how the rest of The Ordeal was received:

Grandmaster should build on Master difficulty, but be significantly more challenging.

Once you reach a certain bar, the challenge should be found completely in perfecting your builds, communication, and execution.

The primary rewards should be doled out on initial completion.

Completion should be the primary metric for success, rather than score or time or another in-activity metric like ‘killing all Champions’

Grandmaster should stress the top end of build crafting.

Grandmaster should be watchable.

To attempt Grandmaster, you will need to be Power Level 1025 which is 15 Power above the Pinnacle cap but will be 25 Power below the activity which will be set at a Power Level of 1050. We’re also going to have something similar to the Contest mode we introduced for Crown of Sorrow. You will gain no extra advantage for being higher than 1025. We wanted to require a mixture of pinnacle rewards and artifact power to get you prepared for this, but not make you feel like you had to put in a ton of extra hours over-leveling the activity. We’re introducing some new modifiers along with some familiar ones, including

Extinguish (if your team wipes, it sends you back to orbit) and Limited Revives (you start with a small pool of shared team revives and you get more every time you kill a Champion). We don’t want to spoil everything, so there are a few other wrinkles we’ll let you discover when it goes live. 

Does this sound like a worthy challenge so far? We hope it does, and as a reward, you’ll have increased chances of being awarded Exotic armor and Masterworking materials. But the main draw of the mode is the new Seal and title available for beating each of the Grandmaster Ordeal Strikes once during the Season: Conqueror. We want this to be one the most challenging titles to get, but we want it to be approachable for anyone to try who puts in the time and dedication with their team during a given Season.

Grandmaster Ordeal will begin on April 21.

The second coming of Iron Banner for this Season is already upon us. Lord Saladin will be setting up shop at the top of the Tower and you’ll have another shot at finishing the quest and earning the new Bow. Bonus Valor gains are also enabled throughout the week.

Iron Banner and bonus valor begins at 10 AM PDT on April 14, running through 10 AM on April 21.

WEEKLY CHALLENGE ACCEPTED

Pleas welcome in the Player Support team with the latest on known issues we’re tracking. This is their report.

TRIALS WEEKLY CHALLENGE UPDATE

Last week, we released Destiny 2 Hotfix 2.8.0.2. As part of the hotfix, we resolved an issue where Trials of Osiris Weekly Challenges were not being reset properly. We believed the issue would be resolved later that week when Trials of Osiris went live, but the issue persisted, causing players who didn’t have their Weekly Challenge to not receive any rewards on their third, fifth, or seventh win.

Starting this Friday, April 10, all players are expected to have their Weekly Challenge available to them when Trials of Osiris becomes active.

CLAN CREATION AND EDITING

Players may notice that they are unable to create or edit their clans in Destiny 2. While we investigate this issue, players can still join or leave Destiny 2 clans at any time.

PC CRASHING UPDATEO

n Tuesday, we worked with Valve to issue a small update to Destiny 2 on Steam that should resolve a crashing issue players have been receiving. This small update is also helping us to determine what is causing the BEAVER error code to appear more frequently.

BEAVER ERROR CODES

Since Season of the Worthy began, we have been seeing a significant increase in the following error codes: ANTEATER, BEAVER, BEETLE, and WATERCRESS. While we continue to investigate the cause of these error codes, we have implemented fixes that have helped reduce the number of BEAVER errors on Steam, which you can see illustrated in the chart below. We understand how frustrating and painful it can be to receive this or any error code, and we’re working intensely on further mitigations. KNOWN ISSUES

While we continue investigating various known issues, here is a list of the latest issues that were reported to us in our #Help Forum:

Fully upgrading any Seraph Bunker sometimes doesn't unlock the associated Warmind Security Triumph. This issue is expected to be resolved in our next update.

Warden of Nothing doesn't give platinum rewards if one of the Unstoppable Champions is killed by a train.

The Dynamic Charge perk doesn’t activate on Symmetry if shot through Citan's Ramparts assisted Assault Barricade.

For a full list of emergent issues in Destiny 2, players can review our Known Issues article. Players who observe other issues should report them to our #Help forum.

#Destiny #Destiny 2 #Destiny 2: Forsaken #Bungie Weekly Update #long post

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ilovemyschool · 4 years ago

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Teaching through COVID???

Bless you if you actually make it to the end of this post, lol.

I teach high school science- specifically Chemistry and AP Chemistry. I absolutely love teaching and I love my students. I especially enjoy getting to talk to them about what they want to do when they graduate, where they want to go to college, what kind of jobs they want to do, and all of that fun stuff. Finishing high school is an incredibly exciting time in life for a person, and I feel privileged to get to re-live the excitement and apprehension and hopefulness and all the other feelings that come along with having so many possibilities for your life laid out in front of you. I don’t know any other kind of work that allows you to feel those feelings year after year like I get to through my students. I also try to support them through the hard stuff. I listen when they cry and tell me that they feel alone in a room full of people, I hug them (if they want a hug) when they tell me their mom moved out over the weekend, and I feed them and get them additional support when they tell me they are hungry and don’t have enough to eat. I spend hours on tutoring, grading, and lesson planning outside of my “contract hours.” It never bothered me because I knew I was doing something that mattered to my kids. If you’ve never gotten to see a kid gain self-confidence in their own ability by practicing with you one-on-one- let me just tell you it’s magical. When they know you’ll sit down and work with them again and again when it’s still tough for them, they can see that you believe they’re worth the time and effort, and they start to believe it too. When you get a note from a student about how they never thought they’d be able to understand chemistry so well, but aced a state final exam or got a 4 or 5 on the AP exam, it feels like you’ve done more than teach them your subject- you’ve taught them to believe they can do hard things.

I’m sick to my stomach right now, because I am so torn on whether to go back this year. My students are set to come back in two weeks. There are so many things going through my head and this has been whirling around for the past two weeks, so I’m writing it out. To quit or not to quit. That is my question.

To Quit:

*My district notified parents of the plan just two weeks ago at the same time as the teachers- teachers actually just got a quick email that said something to the effect of “oh hey- check out this stuff we’re sending to parents about next school year.”

*Since they released their plan, I got in to see a doctor. I have an autoimmune condition. It’s not a big deal in general, just a pill everyday, but it does affect my risk- although in the grand scheme of immuno-issues, thankfully mine is on the low end of the COVID risk spectrum.

*The district’s plan is for all students to go back to school 5 days/week, unless they opt for the virtual option. The hours will be shortened so that the district doesn’t have to do a deep clean at the 4 hour mark as would be required if we were in school for the usual 7 hours. Instead, teachers will all teach 4 class periods and also have to teach an online class. If you’ve never taught, teaching online is a whole separate thing, so even if you teach chem both online and in person, it’s likely that most of the time you’ll have to set up your lessons completely differently for the two. It’s not a deal breaker, but it’s extra work for sure.

*Teachers are responsible for sanitizing the classrooms between classes, which means we’ll have to pee some other time, although every teacher is teaching all 4 classes, so we won’t have anyone available to cover us? I guess they’ll figure that out?

*According to the FAQ document our principal sent out, if we are told to quarantine or isolate, we have to use our sick days. If we go through our sick days or run out we can apply to the sick day bank. They don’t say it in the FAQ, but once you’ve used up days, they dock your pay.

*However, that might not actually be a problem, because in a virtual staff meeting they held on Friday, the assistant superintendent shared that the health department here is now defining “exposure” as 15 minutes or more within 6 feet of a person who has tested positive without a mask. That means that we could be in the classroom with kids who later test positive for COVID for an hour and neither the teacher nor the parents of the other kids in that class would be notified or asked to isolate because we were all wearing masks and therefore were “not exposed.”

*Since all kids are going back at the same time, thats nearly 1800 kids (minus the ones who signed up to take all their classes virtually). Based on early estimates, less than 20% are going to opt to go online. There are no plans to stagger class changes, which means our hallways will be full- it will not be possible for students to social distance.

*Currently, I have a class with 33 students in one of my face-to-face classes. That’s a fairly big class anyway, but in COVID, they’ll be packed in there. It is not possible to keep that many kids 6 feet apart in my classroom.

*We are relying on parents to do temperature checks every day and keep their child home if their temp is 100.4 or above. If you’ve ever taught, you know that while most parents are responsible with things like this, there are some that will send their child in no matter what because they have to work or (in some very sad situations) want the time to themselves.

*In our state’s official COVID school plans, they outlined “Required,” “Strongly Recommended,” and “Recommended” measures. My district seems to be reading “Strongly Recommended” as “Not Required.” This means that they are okay with us running labs, sharing equipment, and working in close proximity because they think that parents understand that if they’re sending their child to school, that they know their child will be in close proximity to others. They say that parents know that their kids will be 2/bus seat anyway and that they’re going to have to be changing classes in a full hallway. I’m not so sure I agree with that. I think parents are probably very unaware of that because I think it would be reasonable for parents to think that the “Strongly Recommended” guidelines would be implemented. I’m not a parent, but I think that I would assume that? Unfortunately, things like 6 feet of separation, doing on-site temperature checks, and not sharing materials are in the “Strongly Recommended” category, which means the district will “do their best.”

*Our district’s Union President wrote a letter to the board on our behalf regarding the strongly recommended guidelines. The superintendent was dismissive of those concerns, stating that schools in other countries saw negligible spread upon reopening, which is like comparing our shitty COVID apples to European oranges. Shortly after his response, two other board members went on to praise the administration for putting together a “safe” plan and quickly approved it to send on to the department of education. I wish that those board members would come and sit in our classrooms for the first few weeks of school.

*We won’t know which class(es) we’ll be teaching online until the week before (best case scenario), so we can’t prepare very much that is specific to our class until the week before school. We won’t know our final schedule in general until next week. To not know this with only a week and a half to go is insane. My anxiety is in full gear.

*Financially, we could handle it if I don’t work.

Not to Quit:

*I have one student who had me for a science class his freshman year, then requested to take my chemistry class during his sophomore year, and is signed up for AP Chem this year. I don’t want to miss it.

*Lots of my former chem kids are signed up for my AP Chem class this year. I’m newer to the school, but I’ve been really working on growing the AP Chem program. We even had enough students sign up to make 2 sections of AP Chem this year, which hasn’t happened in a long time at this school.

*I don’t want to quit with only 2 weeks before school- granted, they just announced the district plans 2 weeks ago and in that time I’ve had to talk with my husband and family, consult a doctor, and look at our finances and upcoming expenses to gather the information I need to make a decision. However, with only 2 weeks left before kids are in my classroom, it would be extremely tight to hire and have someone in place for those kids. I would hate to leave students in that spot where they might start school with a sub.

*I LOVE my classroom and my lab. I put so much time into organizing and cleaning it out. I decorated it really nice and made it super functional. I would hate to have to move everything out- I doubt I’d ever have a classroom that epic again. All my desks match, too!

*A bird in hand is worth two in the bush. I have a job I really love at a school I like and with kids I like and it’s close to my house. If I resign, they’ll have to hire someone else for my job, and I won’t get it back next year. There is no guarantee that I get hired again next year at another school nearby either. With budget cuts, who knows?

*In a new job, I could be teaching anything in the sciences- I love that I have a specifically chemistry teaching job. Those are rare and hard to come by.

*One of the “Required” measures in the state’s plan is to wear a mask. That’s helpful. All students and staff will have to wear a mask unless they are medically exempt.

*I’m still youngish, especially by COVID risk standards.

*Maybe nothing bad will happen- hopefully it won’t and the year will go relatively smoothly and staff and students will stay healthy and get through unscathed. If that ends up being how it goes, I’d regret resigning and second guess my decision.

*I would feel guilty for calling it quits when so many others don’t have the option and may be at higher risk than me due to age or underlying conditions or taking care of loved ones that are either older or immunocompromised. I know so many teachers who have to work this year because their spouse/partner is unemployed, or they are the sole breadwinner for their family, or they are going to retire soon and need their income to stay high to maximize their social security benefits.

*I don’t know how I’ll take it if I go from teaching full time to being a stay at home wife. I did stay at home for a year when we moved to another state, and it was HARD on me. I developed a bit of a depression, exasperated by some other things that were going on. I got on medication and did some therapy and it eventually resolved, but that SUCKED. I would really miss my students and my fellow teachers and having a clear purpose/mission for my days.

In conclusion...

I’m not generally a hypochondriac or a “Nervous Nelly.” Most stuff rolls off my back fairly easily. This scares me. I get the flu or an upper respiratory thing almost every year. There’s no reason to think that somehow I’ll manage to miss COVID if it comes into our school. I am beyond anxious about teaching in person with so few precautions being taken. I’m also angry that my choices are to resign and lose the job I really want or to go in and feel anxious and angry about the lack of care and respect that teachers and students are being shown by district and building administration for the foreseeable future until COVID is over. I have had a stress knot in my gut for the past two weeks over this stuff, and I highly doubt it’s going away if I decide to stay and teach.

Since the pandemic started I have stayed at my house with few exceptions over the summer. I wear a mask when I go out, I usually use a pick-up option for my groceries, a drive-thru option for my pharmacy, and I just avoid gatherings. We do occasionally see my in-laws and my parents, usually outside and observing social distancing. In my state restaurants can’t fill to more than 50% capacity and movie theaters are just plain closed, but schools are about to open at 100% capacity. I honestly can’t imagine putting myself in an enclosed space with over 30 kids or into a hallway with close to 1800 of them. Even more than that, I can’t imagine not sitting down at a desk next to them to help them or watch them work a problem to see what they’re thinking. I can’t imagine not getting to hug the girl who’s mom left or sit with the boy who doesn’t feel connected with his peers so he comes up to sit with me and do his homework after school. Even if I do teach this year, I worry that my kids won’t get what they need from me- whether that’s homework help or emotional support.

If you are so inclined, please send up a prayer for state leaders, school administrators, teachers/school staff, and students this year. We could all definitely use some wisdom, some grace, and your good vibes.

#teacher #school #back to school #covid #covid19 #covid-19 #tough decisions

8 notes · View notes

stone-man-warrior · 4 years ago

Text

February 13, 2021: 3:00 pm:

====================================================

I have a example of Gnosis that was presented to Los Angeles Unified School District students in the early 1970′s.

This Gnosis inclusion in printed required reading material may be possible to find and study further, was a “Life or Death” sort of a COVID Test in the 1970′s where a book report could get a elementary student killed if they say the wrong thing in the report.

The assignment was to read Lord of the Flies by British Author William Golding, then to write a book report about the events that those young stranded people faced while trapped on a deserted island after their airplane crashed there.

I think it’s last final chapter in the book (is) where the Gnosis shows up, and, it could prove to be that entire novel is a work of Gnosis for weeding out non-paratrooper Canadian terror soldiers who landed in great number in San Fernando Valley California in 1970 - 1971.

That last chapter included that at least two people on parachutes had come out of the shy (sky) and landed on the island where Lord of the Flies took place. The parachuting people did not land, but fell, is the way I remember it, and they had gas masks on when the children found them there, as they had hoped some help had come, it was some other thing, not help, and the children spent some time trying to determine who the dead parachuting guests were.

So, the way I did the report, is I read the book twice, then I decided that the part where those people came parachuting out of the sky was a mistake made at the printer, and I approached my book report as if that chapter belonged in a different book, not Lord of the Flies, was a mistake, and that I had somehow managed to pick up a defective book to do the report with. So, my report stopped abruptly at the close of the chapter before the one when those parachute wearing, gas mask donning intruders had come to the island.

The teacher asked about that, why I stopped without including the last part of the book, and my response was that the book was wrong, my book was defective and contained parts of a different book, so, I wrote about the other parts of the book. I got an A on the report. I lived. Others at the school began to vanish, all of my friends were said to have moved away to other places.

Some things to consider about the usefulness of such Gnosis, rely’s on real knowledge, only those who know that thousands of paratrooper terror soldiers landed in Southern California in the 1970′s will understand or be willing to consider why Lord of the Flies is only one of many ways for the terror leadership to reach the terror army that landed there. The paratroopers in Southern California came in tandem, two per parachute, one adult male, and one child on each parachute. The children ranged in age from about 8 years old to about 12 years old. The children started attending Los Angeles Unified School District Schools. Some of the paratroopers did not land safely. Some got hung up on power lines, some of the parachutes failed to open, and some were injured simply because it was dangerous event.

That report assignment based on a book where the premise is about a crashed airplane filled with children on a deserted island where a “Pig” is used as a religious figure head among the stranded group, and so many other details, all serve as fodder for a child terror soldier to say details about their presence, while writing a book report to a terror teacher substitute while the real teachers are away at a educational enrichment “inservice” day somewhere else. That book report gave opportunity to write something about who made it alive, and who did not. If there were injured terror soldiers, that book report was a way to say who and where the injured, or dead ones, were at, and about where parachutes could have been lodged in trees or power lines or other places where they got hung up on the way down.

I once found one of the parachute harnesses, not the chute. That one I found in a remote place at the east end of DeSoto Ave where there is a very old dam structure made of rocks at Browns Canyon Road where the 118 freeway overpass is at, in 1978, about seven years after the paratroopers landed. I’ll describe the harness when I get an interview from US national security personnel.

Think about that Lord of the Flies Gnosis assignment, and all of the simplified details I provided for you here, to see how Gnosis is bad for Freedom, and serves the terror pirates.

I have a lot to say about my youth in Southern California, but no one to say it to.

===========================================

4:03 pm:

Do a Bing search for “Map of Quebec”:

It brings up this image:

Wait about three to five seconds, and the internet terror pirates put an overlay on top of the map you want to look at, the overlay erases the word “Quebec” and it happens live, as you are looking at it.

Later, when the information is shared, like I am doing, Justin Trudeau will go hide under his house in the basement and call his national Canadian Security forces to say that the information showing that the word Quebec has been erased was done because the person who presented the information is planning to explode Justin Trudeau, in Quebec, and that is why he is hiding in the basement under his house.

If the overlay is put on my view of a search result to cover up the word “Quebec” then it will happen to anyone who has drawn the attention of global terrorists such as Justin Trudeau.

For the record, I don‘t have any desire to explode Justin Trudeau or Quebec. That is not my job. I do think the world would be far safer if Quebec and Justin Trudeau exploded, either on their own or by actions taken by Global Security Forces. But like I said, that is not my job, others are in charge of that kind of thing. I am only an elderly disabled man who is a Medicare beneficiary, so, I can’t be expected to do that level of Global housekeeping, others who better equipped, and in better health are responsible for ridding the world of places like Quebec.

This is also a place of interest, the whole thing with exception of some parts of Ireland is the way it looks. I used to say Scotland was not of interest, but that changed, it’s all bad news over there for far too long... where is my eraser?

This has always been a big problem for the whole world. It’s a boat, sink it.

And this is the main source of all of the problems on earth.

It’s just a little tiny place, see below:

This is all it is, and it’s destroying everything else:

It’s a book, burn it.

=================================

4:57 pm:

Revisit this just for a minute. This is really too depressing to really do an the in depth report and decode that is warranted here, so, I’ll give you a head start, something to look at as a place of basis for your own decode work.

youtube

https://www.youtube.com/watch?v=00ReU6IGACo

First, some background is necessary:

(when i do strikethrough that is example of Christian terror at Centurylink changing the text I wright to a “The” for “Theology” means “God”. There are thousands of places where “That” gets changed to “The” by the terror army operatives at Centurylink, Google, and Tumblr terror cells. It’s the same as if the Pope came to piss on the things I wright while trying to get some help)

To see what is happening here, you have to know the (that) when a citizen is awarded a disability status, that event is called “Award of Disability” and beneficiaries receive an “Award Letter” to inform them that Social Security Administration has finished doing their assessment on the application process that people have to go through, it takes more than two years to complete the process, and EVERYONE is denied in the first round, to discourage those who may be trying to deceive the application process. Once “Awarded”, the person becomes a Medicare Beneficiary, and begins to receive a nominal amount of income based on the amount of Social Security Monthly Premiums that person paid, automatically, as it was deducted from their paycheck throughout their lifetime. There is a maximum to the income amount, it’s not enough to survive on for most people. During the first fifteen years of Disability Award Status, those people are subject to the whims of the SSA, if they feel like a reassessment is necessary, the person is called in to a hearing, and must PROVE that they still should be considered as a Disabled Citizen. It turns out that other neighbors are often a threat to such people, and will go out of their way to make life more difficult for disabled people, and will call the SSA to tell them that their neighbor claims to be disabled, but does not look disabled. That means that the neighbor, who is not a doctor, works at McDonald’s as hostess, can make a problem for the disabled persons. You might say that should no problem if the disability is real, just prove it, again. What you don’t understand about that is the lack of control, the threat of having to pay back all of the income that was received before the neighbor called SSA to say stuff they know nothing about, the worry, extra expense, and most of all the time that is required to focus all of your life’s efforts on proving once again that an Award is to be continued. Every other thing a person may have going on, has to stop, all focus shifts on maintaining what is already in place. It’s like you are out at sea, and the information is such that someone is going to take your boat while you are ten miles out in the water. You have to stop everything to save the boat.

Then, for purpose of that video, after fifteen years passes, disability award citizens are no longer subject to any kind of interference from SSA for review no matter what any one says. So, the disabled person will never again be called in and forced to prove anything after fifteen years passes. That is what Jeff Kiesel is talking about in that video after he introduces the “Dotted Line” where a design patent contract is a protective measure. He is pointing out that it is not likely that anyone will be looking at, or interested in disabled people after fifteen years of awarded disability status, and that fact makes them good targets for the long haul where the victims income can easily be maintained after Jeff Kiesel signs the dotted line where the Guitoligist, Brad, does the dirty deed, Gain Cheap, on the Clean Channel.

Contract; Protection; Design.

Those are among the key statement jargon, where “design” is in reference to subjects suitable for surgical experimentation. If not experiment subjects, then such people can be held captive by someone who claims to be a son or daughter or other relation to the victim, and used for things like taking to a SAG friendly doctor so that Jeff Kiesel and Brad the Guitologist can get high on the captive patients pain medicine that is prescribed after a fall down the stairs, or a “gardening accident” in the yard, while those patients never get the medicine they are prescribed. Hear Jeff Kiesel say the phrase “One Leg” to get an idea of the horror of being held in captivity by drug addict SAG members.

Refer to the 6:34 mark in video to get to the heart of the coded message.

It’s coded. You decode it yourself, to keep your parents and grandparents free of captivity, and yourself, because you never know when that freight train is going to run you over until after the train goes by.

All of that is talked about openly on Google/YouTube.

You watch this video and then argue that Google is not in the snuff movie business, I double dog dare you to.

They even know and mark the video with a warning, so, I’ll warn you also: You cannot Un-See this video. Once you have seen it, it will stay with you forever, like a heroin addiction is to a SAG member.

https://www.youtube.com/watch?v=IZYDc_yR0qA&bpctr=1613268727

This video worked and was playable here on tumblr for a few hours, someone had to manually see that it’s here, then put the age restriction on the Google Snuff movie service. I recommend using someone else’s computer to view the video, as Google has turned it into a controlled environment where they can trace your address, so, use a police computer or one at the local church for viewing Google Snuff Movie Service Productions whenever possible. If you click the link, they will assume you read this account, if that happens, you will be marked as someone who knows the truth about Google and the Vatican, and they will hunt you down, take you captive, torture you so you will give them addresses of your family, especially small children, and your banking and asset access information. Then, they will put you into a commercial grade Chipper/Shredder, and grind you up into a liquid, add some water, and some seeds, and spray you onto the roadside as Erosion Abatement for profit because the Department of Transportation pays the contractors for the service of doing Erosion Abatement work on the roadsides, so that the road won‘t wash away in the rainy season. That, is the Christian Way. You can‘t see it through so many daisy’s is part of the problem with that. Orange poppy seeds are most popular in Or/egon for the Human Erosion Abatement Program. The mixture starts out as “V-8″ they call it, then when the seeds are added, at the time of the spraying, they call it “Red Hydroseed” and that is what the State is billed for by the contractors. no, I am not joking, does it sound like a joke?.

youtube

The reason that video exists and is presented on Google/YouTube, along with other similar ones, and movies of animal torture, is create a market for them. The existence of such movies available mainstream makes a “custom order” situation available through YouTube and it’s all approved and signed off by Sundar Pichai, and the Pope with use of a warning label, and the added benefit of that SAG Media “Color Announcer” who calls the action like a little league umpire calls an infield fly rule. Those who are really in the know, knows that the snuff movie presented, is about young boys and young girls because of the “Infield Fly Rule” that is presented in the approved Google snuff production there. “Infield Fly Rule” at a little league game is far more difficult to call as a umpire, than that snuff movie Color Announcer does with a train.

Boy Scouts of America

Eagle Scouts

Girl Scouts

Brownie’s

Little League Baseball

Pop-Warner Football

Gymnastics

Any and all extra curricular sports activities where SAG members can sign up to be a coach or a mentor, all inclusive, is what the train video is truly about, and is what Google is truly about.

Learn to read terror comm.

Turn off the fucking television, there is nothing real presented on it.

Stop the terror take over of USA. Preserve USA by restoring the Freedom that was lost to the Christian terror pirates.

Expose them, remove their fake Corona Masks.

Make arrests. Take them all to Easter Island and drop them off there. There will be way too many to put into the prisons.

===============================

6:46 pm:

Local Conditions:

About 40 degrees F. Absence of wind, high overcast sky.

I saw lights moving around in my front yard as dusk settled in at the close of that Boy Scouts entry above.

I took a walk to the mailbox.

I hesitated momentarily at the front door, opened and closed it a couple of times knowing that the terror bastards are triggered into action with listen device they put beneath my house at the entry.

I stepped out and a car moved south on Russell Road, as is usual at this time of day when I step outside, I am a little early with walk today because of those lights I saw in the front.

There were no lights on at the 376 Jackpine unit B as I stepped over to the driveway, there is a odor of death there, is faint, smells as if a dead creature or persons is in wooded area over there near the Offensive Monroe Surveillance Travel Trailer. I continued to the road, as I passed by the Monroe’s camara area pointed at my gate, there was the sound of jet ... I looked all over the sky to see the jet, but there was no sign of airplane, the sound went north, then west, then north again, then south, it was not a jet, it was a terror soldier’s nitrous gas tank ignited by my Bic Lighter and the sound of a launched terror soldier.

There was one item at my driveway entrance that was out of place, someone had moved a thing that I don‘t think could have moved on it’s own.

The people at 445 Jackpine have all of the house lights off tonight, is totally dark in contrast to last night when the place was lit up brightly. I could see that one of the vehicles there is a pick-up truck, looks medium grey color, but it’s too dark outside to know more, or it could be blue color.

There was nothing in the mailbox.

That Mazda, or other similar looking car was at 520 Jackpine again, parked in front, there was a dog in the yard, and someone was hiding behind a wood fence there along the road watching as I looked for my mail in the box.

I went over that way, there should have been some trash cans brought to the corner by now, in anticipation of Monday Trash Day, but there were no trashcans out on a Saturday, is unusual but not unheard of.

As I looked around at the corner, another terror soldier ignited at 520 and must have taken off very quickly, that man hiding by the fence came out and got into that Mazda, and drove away while I was still walking on the road. He used a flashlight to light me up, so, I returned the light back to him, with a bigger, more powerful light.

I was concerned for a moment he might try to run me over, but I just stayed my course, and went to my driveway. I think the man in the Mazda thought I was the Jet I heard, his accomplice terror assassin, or maybe the other one that was standing right next to the Mazda and launched away quick. I think that one landed at 535 Jackpine at Freeberg terror cell, as I did hear a “Thud” sound from that direction.

It looked to me that the Mazda went to 376 Jackpine to Chartrand terror cell. I did not stay close enough to know for sure though.

There is a possibility that the car was brought to Chartrand by remote control, that is not uncommon, and all of the automatic transmission cars are fitted with remote control operation so that in event that the gas wielding terror soldiers are ignited and burst, the cars can be driven remotely to a controlled place to clean the guts, piss, and shit that gets spattered all on the interior of the cars when that happens. The Myers car from 560 has had dozens of Bursted terror soldiers explode in it over many years.

The lights at 376 Jackpine unit B came on at the front entry there as I walked back to my house.

Conclusion is that all four of the 445 Jackpine assassins are all dead now, and as I thought, were working with the people at 376, and, 376 is the Mazda that has been bringing dogs to the 520 address on occasion for staging attacks at my house with help from many terror cells from near and far.

I’ll take another walk later on to see if Mazda Man wants to dance with me...

Here kitty, kitty, kitty .... Ohhh Mazda Man... Let’s Dance!

==============

7:27 pm:

In event that nsa is watching, wondering, learning, be advised of the VKA follow up work I did earlier today.

Can’t say more.

also: 928-249-3186 Kingman AZ Jeremy is of interest, sent him the 1 2 3 in return for A B C he sent last week about a “SpringBoard”. I could use some help with dealing with Jeremy.

https://www.youtube.com/watch?v=bQtPzo-7AHs

youtube

https://www.youtube.com/watch?v=bQtPzo-7AHs

Possibly Jeremy Barns. A former renter of one of my houses many years ago, associated with Marc & Kayla Cobb who are members of the Tucker’s Barber Shop on 6th St. at Village Square Shopping Center, a “Hawaii 5-O“ terror cell controlled by “Greens of Olde Three Ply” Vatican Choir high command at Kauai Ranch. Jerremy Barns is also loosely affiliated with Joanne St. Cyr of Quebec Canada, and she is part of many things, Jazz Airline is one of them, a Air Canada regional commuter airline serving the Quebec/Montreal area.

Jeremy Barns, The Cobb terror cell, Todd & Alicia Wright and Micheal & Mercedes Wright terror cells are all Safari terror cell members, the Wright’s are actual Lion Handlers, and the Wrights are family of Richard Wright of Pink Floyd, who is one of the Green’s of Olde Three Ply Vatican Choir terror high command at Kauai Ranch.

I suppose all of tonight’s walk to the mailbox is just for personal documentation so I can remember things as they occurred, since no one is interested in preserving USA, or restoring lost Freedom enough to ask me a question about this account of terrorism on Tumblr.

===========================

9:49 pm:

I am tired, I don‘t want to do the decoding, but you can.

Here, this man runs down all of the necessary ingredients to say: “Last in Line”. a Ronnie James Dio song that is deep, but partly is about a entertainment event where terror soldiers go to the back of the line at the entrance, they have swords, a lot of protection from event staff at a concert, baseball, football, any kind of event where the venue has a “Grey Area”, a median place between the seating area and the entrance to the venue such as the concessions area at a baseball stadium is where you present your ticket to an usher before the event begins. The terror soldiers “Hold their swords horizontally, and move forward” through the crowd that is lined up in such a “Grey” area. The audience is killed before the show begins, three percent of the total show audience is taken, ID’s are processed, and replacement look-a-likes are arranged to go live at the victims homes, ultimately, to vote for SAG Shills on ballots such as are all of the people who are featured at the fake impeachment hearings. He says everything but “Horizontal” in coded language.

https://twitter.com/ABC/status/1360677045139869702

Here, Twitter covered that missing “Horizontal” twice with trending Letterman bullshit. Here, he “Roasts” Lindsey Lohan to a point of tears, she must have said or done something to gain attention to the ways of the Screen Actor Guild and was punished for saying whatever she had said, maybe some research can find what Lindsey revealed.

She is wearing “Upholstery”, basically is donned in a sofa. So, the Upholstery outfit could possibly be enough to piss David Letterman off, and Roast her.

Upholstery = “There must be some kind of hold up” in SAG terror language, is a Universally used term, many uses. She may be using it as a “USA is being hijacked” sort of holdup. So, they are sending her to Rehab, where she becomes the “Horizontal” member of the impeachment asshole above who is saying “Last in line” and it’s also a “Sloppy Seconds” statement.

https://twitter.com/davejorgenson/status/1360783863887376390

This at the end is a place where Dave threatens Cher (who does not look exactly the same as I recall she looks in that interview) and that video clip makes the horizontal part of the impeachment Last in Line happen twice, with a “Sloppy Seconds” at the end of the show.

It’s complicated, but not impossible to decode David Letterman, besides, he’s been dead for about five years, his head was put into the mailboxes on Jackpine after the show was over.

https://twitter.com/Squidwardsnose8/status/1360696495708778504

One of the more important things to keep in mind is that Twitter made all of that click together, with use of “Twitter Trends”. The content of the Tweets from Letterman are all contained in the vast archive of Twitters digital storage capacity, and Twitter is Google, so arranging all of that stored archive to produce desired results is what Google is all about, it’s what they do, they categorize information.

So, once you do the decode, then you need to see why the decode opertunity presented itself there for you to see. It was Twitter that arranged all of that so it would be there to use as a language, a graphic based language that says more than the individual pieces of video clip when combined, and with a host from the fake impeachment to guide the reader to the message.

The message is about a mass murder at a venue, where there are women who will be punished, captured and killed after plenty of raping is done by David Letterman and Jay Leno, who you know is waiting to share a garage with Dave nearby.

My read is a personal one, that includes much other communication on Google products, in the music industry email promotions, and in my neighborhood, where the message spells out a Rush at my house on Sunday morning by men with swords, and with horses, equestrian snuff at my house, with a draw and quarter featured at the end of the show.

I can hardly wait.

Draw & Quarter: It’s been about fifteen years since the last time I saw someone being drawn & quartered. Usual is done with two horses, where some ropes and shackles are attached to a victims arms and legs, and the other end is tied to the saddle of the horses. They prefer to use four horses, but one will work if horses are in short supply, just tie the other end of the rope to a tree and the result is almost the same. The horses slowly pull the victim into pieces.

The Draw & Quarter events are very unpleasant to see, even worse to be the star of the show.

Best guess is Adrian Witcherly will supply the horses. She is a bank manager at the Midland St. Branch of JP Morgan Chase Bank in Grants Pass, corner of 7th St. not far from OR State Police Field Office which next to the Seventh Day Adventist Church on 9th St.

=====================

11:00 pm:

This man is said to be the most powerful man on earth, surely he will save me, and prevent the event from happening, given that all of information necessary for prevention has been provided.

I am confidant that the US President will do what ever is necessary to prevent tragedy when he has opportunity in advance to do so.

Certainly he will realize the horrible threat to USA, to Freedom, and the threat to mankind that is presented to the world by Google, and will everything in his vast power to stop Google and Twitter from murdering more US citizens forever.

Joe Biden will do the right thing, he has been informed of the danger.

https://twitter.com/POTUS

=======================

11:33 pm:

I just now sent this cry for help to the White House.

2-13-2021 at about 11:27 pm Oregon time.

Take note that I specified to read the information in the links provided, and that 911 Emergency phone service will produce assassins at my house if I use that. I have much other documentation of failure at FBI.Tips.Gov too. It does not work, only assassins come in response to reports of terror mass murder in Oregon.

“Please send help. The state of Oregon has been hijacked by terror army from Canada, they use poison gasses to overpower victims, capture and torture them, then kill & replace them with imposters. There are many hundreds of thousands of them, and they have been murdering the population for more than twenty years. There are more than 800 pages of explanations at the link provided, eye-witness accounts of actual terror mass murder happening in Oregon and all over the west coast of USA. Please study the information carefully, as the terror is very sophisticated. 911 Emergency phone service is not available, it's controlled and operated by the terror army, they only send assassins when people call for help. Please send US Military. https://stone-man-warrior.tumblr.com https://stone-man-warrior.tumblr.com/archive”

They will call the local sheriff.

The local sheriff will send assassins.

That is what happened each time I sent a request for help to a US President.

Obama.

Trump.

Biden.

They have all been informed. none have stopped the terrorism, mass murders, kidnapping, or US takeover.

They all have sent assassins.

Joe Biden started sending assassins before he took office.

If I disappear, Joe Biden’s people did it.

============

11:46 pm:

Here is the BBC UK news response to my letter to Joe Biden at WhiteHouse.gov:

https://twitter.com/BBCNews/status/1360856008969621507

This is the part where Reuters UK (SIS MI6) makes the order to change the contact information I included with the note to Joe Biden at WhiteHouse.gov. This effectively will result in the death of investigative persons sent to the wrong places, because that Tweet says to make “Variant Modifications” and is directed at what I posted regarding the note asking for help.

I used the correct contact information on the form at WhiteHouse.gov. Part of the terrorism includes that the phones don‘t reach the people I need to reach, only approved terror cells can be reached with a telephone, and only terror cells call my phone number.

It’s should not be difficult to see where I live to contact me by reading the information at the links included, to this account. The problem with that is the investigative persons are not provided with the source information and have no idea that this account exists at all, they just go where the leadership tells them to go do investigative work, but the leadership is all SAG Shills, are all terror army operatives, and they send the investigative people into traps intentionally. Reuters UK is making sure that investigative persons go to Bullhead City Arizona, instead of Oregon where I am at, and where I can help them help me to help everyone, and restore the freedom that was lost so long ago.

https://twitter.com/ReutersUK/status/1360857587453988866

February 14, 2021: 10:05 am:

February 14, 2021: 10:38 pm:

https://twitter.com/POTUS/status/1360990937606983691

Biden effectively says: “Fuck you minion”

Then turns to his Canadian SDA terror soldiers and commands: “Grab your rakes and pitchforks, let’s get to work”

https://www.youtube.com/watch?v=BniO017oeTM

youtube

The President of the United States of America, Joe Biden.

It’s St. Valentines Day today, I forgot, he’s busy ... today is a bad day for doing anti-terror work.

1 note · View note

crescentmoon223 · 5 years ago

Text

When This is Over

As promised, my Stella/Scully quarantine fic. Heads up, it’s SUPER SMUTTY - you’ve been warned lol.

Tensions run high when Stella and Scully are forced to self-isolate together at home during a global pandemic. How far will Scully go to convince Stella to take her on a belated honeymoon once this is over? (Hint: bedroom hijinks!)

Read it on AO3 Note: This is set in present day (spring 2020), post Two Worlds Collide and after their wedding. When I write the sequel to TWC, it will be set during the summer and fall of 2019, directly after the epilogue (and yes, it will include their wedding!) Sorry if this is confusing, but it’s the only way the timeline made sense in my head lol. Obviously, I would rather write the TWC sequel first (and I do hope to write it next month!) but the idea for this quarantine fic hit last weekend, and I decided to go for it while it was relevant. I hope you enjoy! xx

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There were certain sounds that Scully associated with this new chapter in their lives. The clatter of fingers on a keyboard. The murmur of newscasters from the television in the kitchen. The flute music Stella listened to while she did yoga. And currently, the bump and clatter of her obsessively cleaning their flat. Scully looked up from her laptop with a smile as Stella entered her line of vision. She wore gray yoga pants with a black tank top, her hair tied back with a red polka-dot bandana. She wiped down their already sparkling countertops, spraying and scrubbing at spots only she could see. Everyday Stella was a neat freak and a bit of a germophobe. Stella during a viral pandemic was next level. “Nice ass,” Scully called as Stella bent to wipe down the front of one of the cabinets. Stella shot her an exasperated look, but she turned, leaning over the sink to rinse her cleaning cloth in a way that pressed her breasts together, emphasizing her cleavage beneath the snug-fitting tank top, and it certainly wasn’t accidental.

Read the rest on AO3

Scully closed out of the software the university was using for its virtual classroom. She’d been teaching forensics online for a week now, and it had gone relatively smoothly, although she missed the face-to-face interaction with her students. Setting her laptop on the table, she returned her attention to her wife. “Planning to stop cleaning anytime soon?” Another sharp look from Stella. “I’ll stop when it’s clean.” Scully knew better than to argue with her. She fought a smile as she remembered the abject horror on Stella’s face after she’d been told that as Detective Chief Superintendent, a position which required little to no field work, she was one of the non-essential Met personnel who would be expected to work from home until the self-isolation period had passed. In the days since, she had quickly demonstrated why she was not a good candidate to work from home. Unable to go to the office—or the pool—she’d covered the living room table with Met paperwork, file folders and notebooks filled with her ingenious musings. When she wasn’t working, she alternated between cleaning fits, ill-fated yoga sessions that tended to end in a lot of swearing and whiskey drinking, and watching entirely too much news BBC coverage on COVID-19. In short, she was driving Scully crazy. For her part, Scully had a more pragmatic approach. She’d suffered through many less pleasant quarantine situations than this one during her time on the X Files, and she’d already faced the potential end of the world as she knew it…more than once. She was perfectly content now to teach from her home office, run her own data on the progression of the virus, and spend plenty of time with her wife. If only she could get Stella to settle. “I need to check on my mom, and then we should start thinking about dinner,” Scully told her. “There are steaks in the freezer,” Stella said. “I’ll put them in the sink to thaw.” “Perfect.” Scully picked up her laptop and opened FaceTime to dial her mom. Maggie’s face appeared on the screen, and Scully felt something deep inside her relax. Probably the hardest part of this mandatory self-isolation was being trapped an ocean away from her mom, who was in a high-risk category due to her age. Thankfully, Maggie had lots of people checking on her. “Dana, I was just thinking about you,” she said with a smile. “How are you today, Mom?” “Oh, I’m just fine. Fox dropped by earlier. He brought me groceries, that sweet man.” Scully’s heart clenched. Mulder was a sweet man to check on her mom for her, not that she’d ever doubted this about him. They were family, in every way that mattered. She made a mental note to call him in the morning to thank him, and to make sure he was taking care of himself too. “He didn’t come in, did he?” “No. He left the groceries on the porch and then sat and talked with me for a while through the window.” Scully smiled. “That was nice of him.” “He talked a lot about increased UFO sightings, particularly in areas with the highest rate of infection.” Maggie’s eyes widened. “Mom.” Scully shook her head in exasperation. “It’s not an alien virus. In fact, it originated from bats.” “Well, he was very convincing, is all I’m saying,” Maggie told her. “He always is,” Scully agreed. “You’re still feeling fine? No symptoms?” “I’m as healthy as a seventy-six-year-old woman could expect to be,” Maggie said. “I even went for a walk around the neighborhood earlier, and before you ask, yes, I kept my distance from everyone else.” “Good,” Scully said, wishing more than anything that she could reach through the screen and give her mom a hug. “Remember, there’s no reason to panic if you do develop symptoms, but it’s extremely important that you get tested right away. Tests are hard to come by in the US, but I know people who can pull strings for you.” “I know, I know,” Maggie said, waving her hands impatiently. “How are you and Stella?” “We’re fine,” Scully told her, glancing toward the kitchen, but Stella was nowhere in sight. “Just a bit stir crazy, but who isn’t?” “I imagine this is very difficult for Stella,” Maggie said with a knowing smile. Scully grinned. “You got that right.” “Did I hear my name?” Stella emerged from the hallway, having changed into a white T-shirt and drawstring pants, her hair loose over her shoulders. She sat on the couch beside Scully, waving politely at Maggie. “Hi, Stella,” Maggie said warmly. “How are you?” “I’m fine, despite what Dana may be telling you,” she said, giving Scully a look. “And how are you, Maggie?” “The very same,” Maggie said with a laugh. Stella joined the conversation for a few minutes before excusing herself to the kitchen to check on the steaks. “I’ll talk to you again tomorrow, Mom,” Scully said. “And call me anytime, for any reason, no matter the hour. Promise?” “Will do,” Maggie said. “Good night, Dana. I love you.” “Love you too, Mom.” She ended the call and closed her laptop. Her gaze caught on the gold band glinting on her left ring finger. She was thankful for it for so many reasons, but right now, it symbolized a vitally important connection between her and Stella. If the worst were to happen and one of them became sick, they would have spousal privileges at the hospital. Stella was her next of kin, with visitation rights and the power to make any difficult decisions that might need to be made. And Scully would do the same for Stella. Both of them had living wills and all their wishes already clearly defined, but it gave her an extra sense of peace knowing they had each other. She touched the ring with a smile. “What do you want to do until dinner?” Stella asked, rejoining her on the couch. “Oh, I’m sure we can think of something.” She smiled as Stella’s fingers traced the seam of Scully’s jeans from her knee slowly up her thigh. They’d always enjoyed an active sex life, but being stuck at home together had made them even more insatiable than usual. She reached for Stella, sliding one hand into her hair as she pulled her in for a kiss. Stella smelled like lemons and soap, her lips soft and pliant against Scully’s. She stared into the azure depths of Stella’s eyes as her mind flipped all the way back to their first kiss against the side of Stella’s car over twenty years ago. They’d shared a lifetime of kisses since, two continents worth, from London to Maryland to Wyoming. Lately, she’d been wanting to add someplace new to that list. She pressed her lips against a sensitive spot on Stella’s jaw, feeling the shudder that ran through her. “You still owe me a honeymoon, you know.” “This isn’t enough for you?” Stella quipped, hands roaming beneath Scully’s shirt. “Endless weeks trapped here at home, fucking each other senseless?” She shook her head, gasping as Stella sucked at the spot beneath her ear that had always been her undoing. “I’m serious. If we survive this pandemic—” “If?” Stella interrupted, her tone sharp. “When,” Scully corrected. “When this is over, we owe it to ourselves to take a real vacation, one that has nothing to do with work or family. Just you and me, celebrating our marriage somewhere special.” Stella said nothing, instead swirling her tongue over Scully’s neck while she teased her through her jeans. But despite her already-wet panties, Scully persisted, because seduction as a form of distraction was the oldest rule in Stella’s book. “Paris,” she said, sliding into Stella’s lap. “There are so many museums we could explore, so much art. The Mona Lisa…” Stella arched her hips so Scully could move against her, friction building through their clothes. “I’ve seen the Mona Lisa.” “But I haven’t.” She slipped a hand between them, touching Stella through her pants, pleased as Stella’s breath hitched, nipples hardening beneath her T-shirt. “Take me, Stel.” “Yes,” Stella said throatily. “Take me to Paris,” she clarified, fingers moving over the soft cotton of Stella’s pants, already damp with her arousal. “We’ll see,” Stella hedged. She thought vacations were a waste of time and money, an extravagance that could be better indulged in fine liquors, fabrics, and other luxuries right here at home. Scully was generally inclined to agree with her. They both worked hard and traveled often enough for work or to visit family that a separate vacation just for the two of them sometimes felt like too much effort. But this was different. She’d already lost too many years to unpredictable viruses and quarantine protocols. She deserved a trip to Paris, at the very least. They both did. But if sex was Stella’s preferred means of communication at the moment, maybe she could use that to her advantage. Scully bent her head and kissed Stella, hands sliding behind her back to hold her close. Her tongue slid into the welcome heat of Stella’s mouth, tasting whiskey. “Picture it, Stella,” she murmured against her lips. “Sidewalk cafés, wine and cheese and melt-in-your-mouth pastries on our balcony.” “Mm,” Stella said noncommittally, hands gripping Scully’s ass. “Versailles,” Scully whispered against the pulse point on Stella’s neck, watching as goose bumps rose on her skin. “Gardens. Flowers. So many beautiful churches.” “I hate church,” Stella said breathlessly. “I’m not asking you to sit through a service with me, just admire the architecture and the stained glass.” She swirled her tongue over Stella’s collarbone, rewarded by a sharp inhale. “Let’s go, when this is over.” “Dana…” Stella’s body stilled beneath Scully’s. “We don’t know when that will be or what the world will look like.” “I know that.” She sat up, taking Stella’s chin in her hand to force her to meet her eyes. “I know that better than almost anyone.” “Then why are you pushing me for a trip right now of all times?” There was a fragility to the fierceness in Stella’s gaze. She was afraid. Scully softened at the realization. “The world will look different when this is over, and it will be a while before we can go to Paris, but it’s good for morale to have something to look forward to, and I would imagine the economy will need a boost from tourism when all is said and done.” “I can find you plenty of architecture and stained glass right here in London.” Stella popped the button on Scully’s jeans, pushing down the zipper with a soft metallic hiss. Scully pressed closer. “Not the point.” “Agree to disagree?” Stella’s fingers dove down the front of Scully’s underwear. Her hips rolled against Stella’s hand as a soft whimper escaped her throat, and a wicked idea took hold in her mind. “I’ll play you for it.” “What?” An adorable wrinkle appeared in Stella’s brow. Scully sat up straighter, grinning. “Sex games are your specialty, are they not?” Stella’s eyebrows lifted. “What kind of game?” “If I can make you come first, we go to Paris. If you make me come first, we stay home.” She dangled the bait, knowing Stella would be unable to refuse, even as she also knew her chances of winning were slim. Stella’s self-control was legendary, after all. But it would be fun to try, and it might even distract Stella from the reality of their situation for a little while. Sure enough, the flame in Stella’s eyes lit. “You’re on.” * * * Stella pushed Scully onto the bed and climbed on top of her, both of them still fully clothed. Now that Scully had made this a game with their honeymoon as the prize, they were both eager to take things slow. Frankly, Stella couldn’t imagine a better way to spend what remained of the afternoon than to slowly and relentlessly tease Scully right over the edge…repeatedly. “You smell good,” Scully murmured, shifting beneath her so that Stella’s right thigh slid between her own. “I took a quick shower while you were talking to your mother.” Stella pressed her thigh more firmly against Scully, rewarded by a little moan. She liked this position, being on top, in control, a feeling that had been in short supply since she’d been sent home from work. Stella hated laying low, waiting the threat to pass. Her every instinct screamed for her to get out there on the frontlines and fight this thing. She’d fearlessly stared down every kind of evil imaginable over the years, but this one was different. There was no bravery in going outside now, nothing but her own stupidity to blame if she allowed the virus to catch her. There was a helplessness to being trapped at home that was slowly eating her alive. She’d cleaned everything she could get her hands on, the only way she knew to fight this faceless threat. She’d checked on Fran nearly as often as Scully called her mother. She’d watched while Scully analyzed articles and data, showing her graphs that scared the fuck out of her, while Scully herself faced their uncertain future with a kind of serenity that made Stella want to scream. Distantly, she wondered if Scully knew all of this, if she’d manufactured this sex game to get Stella out of her head for a little while, to give her a sense of power here that she couldn’t find elsewhere right now. It wouldn’t surprise her, but she wasn’t going to let it ruin this moment either. She slid a hand beneath Scully’s top—a crisp black button-down she’d worn for her online teaching earlier that day—and cupped her over her bra. She brushed her thumb back and forth, feeling Scully’s nipples harden beneath her touch. Scully deftly untied the drawstring on Stella’s pants, slipping a hand inside. “Stella,” she said in a scandalized tone. “Did you FaceTime my mother without underwear?” “Easier to get undressed afterward.” She swallowed a whimper as Scully’s fingers slid over her bare skin before retreating to palm her equally bare breasts. “I’ll say.” Scully pushed Stella’s shirt up, helping her slide it over her head. Stella straightened, tits bouncing as she rocked her hips against Scully’s. The coronavirus was outside her control, but this—her body, her pleasure—this she controlled. That she would win was already a forgone conclusion. She could hardly believe Scully had even offered the challenge. “This might be a better view than anything I would find in the Louvre,” Scully said, gaze locked on Stella’s tits. “All the more reason not to go.” Stella began unbuttoning Scully’s blouse, pausing after each button to kiss the newly exposed skin, leaving a wet trail down the center of her chest and over the clasp of her bra. She continued all the way to the waistband of Scully’s jeans, her shirt now hanging open at her sides. Scully looked down at her, chest heaving, bottom lip pinched between her teeth. “Well, don’t stop there.” “Wasn’t planning to.” Stella eased the shirt down Scully’s left arm and then the right before tossing it to the floor. She helped Scully wiggle out of her jeans, dipping her head to kiss her through her underwear before she stripped those away too, followed by her own pants. Once they were both naked, Stella turned her attention to worshipping every inch of Scully’s bare skin. She kissed her breasts, teasing Scully’s nipples until they had tightened into tight rosy buds, which she flicked with her tongue until Scully moaned. Stella looked up and caught her gaze as she began to kiss her way down Scully’s stomach, making sure to pay special attention to all of her most sensitive spots, like that little patch of skin just below her left hipbone, the one that made her whimper and squirm as Stella’s tongue swirled over it. “God, Stella,” Scully groaned, hands fisted in the sheet. “Mm,” she murmured as she flicked her tongue against Scully’s clit, causing her hips to buck upward with a gasp of surprise. Stella was torn between the desire to drive Scully straight over the edge or to hold herself back a bit and at least give Scully a fighting chance. In the end, she wanted this to last as long as possible. Power games in bed were a huge fucking turn on. Just thinking about what was to come had her throbbing in anticipation. She teased Scully with her tongue, licking and sucking but never giving her as much pressure as she knew Scully preferred. She pushed a single finger inside her, thrusting in time with her tongue, toying with her. Scully’s gasping breaths became increasingly labored, her hips shifting restlessly, her arousal fueling Stella’s. She reached between her own thighs, pressing two fingers against her aching clit. She stroked herself once, twice, just enough to give herself momentary relief from the tension building there before returning her hand to Scully’s body, letting her own need drive her as she focused on her wife. She nipped Scully’s clit, drawing a strangled cry from her lips, before trailing a string of wet, open-mouthed kisses down her inner thighs until Scully was writhing beneath her. “Stop,” she mumbled, pushing at Stella’s shoulders. Stella smiled as she complied, sliding up Scully’s body to kiss her on the mouth, tongues tangling, bodies pressed together from head to toe, spreading heat with every movement. They kissed for what felt like hours, and Stella was lost in the sensations, the warmth of Scully’s breath on her cheeks, the weight of Scully’s breasts against her own, the teasing rub of her skin against the parts of Stella that ached for release. And then Scully was moving, shimmying down to position herself between Stella’s thighs. She inhaled sharply, everything inside her tensing in anticipation. Scully was talented in too many ways to name, but the wicked pleasure of her mouth was something that still overwhelmed Stella in the best possible way. She began with one long, slow lick, and Stella felt herself arching off the bed, her body instinctively seeking more. Scully centered the heat of her mouth over Stella’s clit, swirling there until Stella was dizzy with desire, unaware of anything but the hot press of Scully’s tongue and the powerful need coiling inside her. She moved her hips, rocking against Scully’s mouth, allowing herself this moment of surrender before she slid sideways, rolling to face Scully. Need pulsed wickedly inside her, invigorating her, burning away the ugly things that had built in her chest during the day. “Touch yourself,” she demanded, feeling a shiver of excitement as she watched Scully push a hand between her thighs in response. Scully stroked herself, slowly at first, gradually picking up speed. Her lips parted in silent pleasure, and Stella watched, entranced. Scully stared straight into her eyes as she moved, pleasure apparent in the flush on her cheeks and the slightly dazed expression on her face. “You too,” Scully said breathlessly. “Touch yourself.” Stella did, bringing a hand between her thighs, which were already slick with a combination of her own arousal and Scully’s recent attention. She kept her touch light, careful not to give herself too much stimulation, not when Scully had already brought her so close to the edge. She skimmed her fingers over herself as her core clenched, seeking the release she so relentlessly denied herself. “How close are you?” Scully asked, gaze dropping to Stella’s hand, watching as she touched herself. “Not very,” Stella told her, which was technically true, although she could get herself there quickly enough if she allowed it. “On a scale of one to ten,” Scully persisted, her own fingers still moving, stroking. “One being barely aroused, and ten being the point of no return.” Stella smirked. “Six.” “Oh.” Scully slowed her fingers, looking pained to do so. “And you?” Stella couldn’t help asking. “Eight.” She whimpered slightly. “Maybe a nine.” “Interesting.” Stella reached for her, drawing her close. She pushed Scully’s hand aside, replacing it with her own, and fuck, Scully was so wet. It sent a hot thrill through Stella’s body, shooting straight to her clit. Swiftly, she rolled, sliding a thigh between Scully’s in a move streamlined by years of practice, intimate knowledge of exactly how to position herself so that their pussies pressed together for optimal pleasure. Immediately, Scully began to move, grinding vigorously against Stella. “Nine,” Scully gasped. “Definitely a nine.” Stella was headed there pretty quickly herself, her clit thrumming with need beneath the hot, wet slide of Scully’s body. She watched as Scully surrendered, shuddering in her arms, her pussy pulsing against Stella’s as she came, whimpering, hips jerking, and it was so powerful, she nearly took Stella over the edge with her. She began to move against Scully in earnest now, chasing her own release. She arched her hips so that her clit rubbed against Scully’s pelvic bone with each thrust, and yes, this was it. This was perfect. So fucking perfect. Need coiled hot and tight in her core. Scully rolled away with a wicked grin. “I demand a rematch.” “Well, that’s hardly fair,” Stella said, attempting to steady her voice as her thighs pressed together uncomfortably. “Not exactly a level playing field now, is it?” “It’s perfectly fair,” Scully told her. “You deserved to start with a handicap.” And well, she had a point. Even in her current state, Stella could probably still win their bet. She blew out a long, slow breath. Strike that. She could definitely still win their bet. She could win it three times over if she had to. The game was all part of the thrill for her, and as long as it lasted, she didn’t have to think about what was happening outside their flat, the invisible threat she didn’t know how to fight. “All right.” Scully gave her a smug smile, but Stella kissed it right off her face as she moved in, getting right down to business this time. She fucked Scully hard and fast with her fingers, not letting up until Scully was writhing against her all over again. Once she had her sufficiently worked up, Stella withdrew her hand, trailing her glistening fingers over Scully’s stomach, thrilled with the way she trembled beneath Stella’s touch. “And now?” she asked. “Where are you on your little scale?” “At least a seven.” She pressed a thigh between Stella’s legs, angling her hips to give them both some much-needed friction. “You?” “Same.” Stella pressed herself firmly against Scully’s thigh, attempting to hold still, but Scully stymied her with her own movements, causing her thigh to rub rhythmically against Stella’s already hyper-sensitive clit. She gripped tighter with her thighs, allowing it…for now. “You’re so gorgeous when you’re this turned on,” Scully said reverently, tracing a hand over the contour of Stella’s face. “Makes me want to tease you like this forever.” Stella exhaled, hearing the hitch in her breath. Her thighs, still clamped around Scully’s, began to shake. Scully’s hips stilled, bringing them both to a gasping halt. Stella unthreaded their legs, channeling the energy inside her into a blistering kiss, her lips devouring Scully’s, tongues thrusting in an imitation of the act their bodies craved. This kiss was deep and ravenous, feeding a hunger that only grew with each passing moment. Stella could lose herself here, every cell in her body achingly, breathtakingly alive, adrenaline coursing through her veins, all centered in the pulsing ache between her thighs. They moved together, never quite allowing their bodies to touch the way they needed, instead letting the whisper soft brush of skin against skin stoke the fire blazing between them. “Stella,” Scully gasped, fingernails biting into Stella’s back, causing her to exhale sharply in pleasure. “Do that again,” Stella demanded, her voice low and hoarse. Scully’s nails bit into her skin, dragging slowly down her back to dig into her ass, hauling her up against the welcome heat of Scully’s body. Their hips pressed together more firmly now, and Stella couldn’t help but gasp as Scully thrust against her. “Fuck,” Scully mumbled, and Stella grinned against her lips. Despite her best intentions, Scully had very little willpower when it came to postponing her pleasure. “Nine again, hm?” Stella asked. “Yes.” Scully’s movements became increasingly frantic. Stella arched her back, separating their hips, causing Scully to swear. But Stella wasn’t quite ready for this to be over. Not to mention, Scully was also incredibly, impossibly beautiful when she was this turned on. Her blue eyes gleamed with desire, cheeks flushed, breath coming in irregular gasps. Stella slid down to lavish her breasts, licking and sucking as Scully writhed beneath her. And then, Scully rose, flipping Stella beneath her and pinning her to the bed before she’d realized what was happening. “Time to get you to a nine,” Scully panted, and Stella shifted restlessly against the bed. Truthfully, she’d already been there a few times, and it wouldn’t take much to bring her back…or to carry her over the edge. She held herself still as Scully crawled down her body, giving her a heated look before she closed her mouth directly over Stella’s clit, sucking hard. Stella’s hips bucked, and she held her breath against the urge to beg for more. Scully was on a mission, her tongue licking, flicking, swirling with such an intensity that for several long moments, Stella completely lost her wits, grinding shamelessly against Scully’s mouth. Scully pushed two fingers inside her, expertly stroking her upper wall, and just like that… “Nine,” Stella gasped, pushing Scully away as her core clenched desperately against the emptiness left behind. “Oh, I definitely like you like this,” Scully said, eyes burning hot as they raked over Stella’s body, scorching her everywhere they touched. It was all she could do not to bring her hand between her thighs and let Scully watch as she fucked herself right over the edge. Instead, she pounced, hands gripping Scully’s hips as she evened the score. She held nothing back, nipping and sucking at Scully’s clit as she pushed two fingers inside her, followed quickly by a third. She showed no mercy, working Scully hard and fast, dimly aware that she wasn’t even trying to slow Stella down this time. Scully came in a wet rush, her pussy clenching around Stella’s fingers as her body shook and her hips bucked, followed by a long, low cry of relief. Stella rolled to the side, one hand already between her legs, fucking herself as hard as she’d just fucked Scully. “Wait,” Scully gasped, rising unsteadily as a smile stretched her cheeks. “No,” Stella protested, even as her hand stilled. She could wait. She could keep this up as long as Scully wanted to play. But she was ready to come. Her body shook with the effort not to. “I won fair and square. Twice.” “Yes, you did.” Scully planted a hot kiss against her lips. “And you deserve to be rewarded properly for your efforts.” “Do I?” she asked, one hand still pressed firmly between her legs but not moving, holding herself on the brink of release. “Yes,” Scully said with a brisk nod. “Hands off.” Stella complied, desperately curious as to what Scully had in mind. She steadied her breathing, trying to relax, pushing back her need so that she’d last long enough to properly enjoy her reward, whatever it was. Scully scooted to the edge of the bed and opened the drawer where they kept their toys. A fresh wave of arousal rushed through Stella, and she pressed her thighs together. As she watched, Scully took out the hot pink strap-on that was possibly Stella’s favorite possession. Scully slipped into the harness before turning to face Stella, pink cock jutting in her direction, and she couldn’t help it. She moaned. If it was possible to come from anticipation alone, this would be the moment. Scully squirted lube onto one palm and slicked it over the cock, warming it beneath her palms with long, sweeping strokes while Stella clenched her fists in the sheet to keep from touching herself. She ached to be filled, desperation making her weak. “Ready?” Scully asked as she crawled onto the bed, and Stella nodded, thighs parting in anticipation. “You’re so wet for me, Stella,” Scully said as she allowed the head of the cock to brush against Stella’s entrance. “Mm,” she agreed, gripping Scully’s ass, pulling her closer. “Impatient, are we?” Scully quipped, rocking her hips so that the head of the cock slipped inside Stella. She whimpered, too far gone to argue. Scully pressed forward, testing Stella as her body adjusted to the toy before she drew back and slid home, filling Stella completely. She moaned in relief, falling back against the mattress as Scully straddled her, thrusting into her hard and fast, just the way Stella liked. Scully reached between them, stroking Stella’s clit in time with the movement of her hips, and Stella was done for. Her eyes fell shut as she rocked up to meet Scully, barely able to breathe past the need rising inside her, throbbing in her core, building hotter and stronger with each thrust until she thought she might burst from the power of it. “More,” she gasped. Scully picked up the pace, pounding into her as her fingers circled Stella’s clit, harder, faster, and then she was coming, arching off the bed with a moan as the orgasm rushed through her, leaving her hot and tingly in its wake. She lay there, gasping for breath, still impossibly aroused by the feel of the cock inside her and the woman on top of her. Scully paused, allowing Stella a moment to catch her breath before she resumed her movements, this time tilting her hips so that the head of the cock rubbed against Stella’s G-spot on every stroke. She swore as need rose inside her again, impossible stronger and more urgent than before, so intense she could only writhe and swear beneath Scully as she carried Stella swiftly toward a second orgasm. “Come for me,” Scully whispered, and Stella did. Her core ignited with release, pulsing through her with such an intensity, she almost thought she was having an out of body experience. A high, keening cry tore from her throat as wave after wave of pleasure rolled through her. It kept coming, she kept coming, as her body released all the tension she’d built up during their game. When she regained her senses, she was limp and shaky, her body covered in sweat, her pussy still tingling with aftershocks of pleasure. “Wow,” Scully whispered. “I don’t know if I’ve ever seen you come that hard.” Rather than answering, Stella pulled her in for a deep, drunken kiss. She wasn’t entirely sure she could form words just yet, let alone move, so she just lay there, panting for breath, as Scully climbed off the bed and went into the bathroom to clean their toy. She returned a few minutes later and tucked the dildo back into its drawer before sliding into bed, a pink cloth in her hands. She pressed it against Stella’s flushed face. The cloth was warm and damp, and it felt so good as Scully gently wiped the sweat from her skin. She worked her way down Stella’s body, washing away sweat and sex. When she’d finished, she tossed the cloth toward the bathroom and crawled in beside Stella, one arm thrown over her stomach. They lay like that for a while, both of them calm and sated. Scully’s fingers traced lazy patterns on Stella’s skin. “We’ll have a lovely honeymoon here in London when this is over,” she said softly without a trace of disappointment in her tone, true to her word. Stella looked down at the gold band on her finger. She tightened her arms protectively around Scully as teared pooled in her eyes. Despite their self-isolation, what if the virus managed to penetrate their home? What if something happened to Scully? Stella couldn’t bear the thought. Scully closed her eyes, resting peacefully in Stella’s arms. Was she daydreaming about their honeymoon? Stella tried to imagine it, but she found herself picturing Scully sipping champagne on the Eiffel Tower, eating pastries at a sidewalk café, spouting scientific details as Stella showed her magnificent stained glass in Saint Chappelle and Notre Dame, so much stained glass it would take her breath away. If they survived this—when they survived this—how could Stella possibly deny her the honeymoon of her dreams? How could she deny either of them that chance? She held Scully tightly, burying her face in the floral-scented depths of Scully’s hair. “But not as lovely as Paris.” Scully pulled back to give her a baffled look. “You were right,” Stella whispered. “We should go to Paris.” Scully beamed at her with a joy so pure, it warmed even the darkest, most fearful parts of Stella’s heart. “Really?” “Really.” She drew in a breath. “But it will be a while before we can go, Dana, and I don’t—” “I know.” Scully brought their lips together for a gentle kiss. “It took us twenty years to get married. However long we have to wait for our honeymoon, it will be worth it.” Stella nodded as a tear slipped over her cheek, knowing in her heart that it was true.

#fanfic #Two Worlds Collide #scullyxstella #stella and scully #scully/stella #Stella Gibson #Dana Scully #quarantine #quarantine fic

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kmsherrard · 4 years ago

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In praise of roller coaster rides

“...the thousand concurring accidents of such an audacious enterprise….”

-Herman Melville, Moby Dick

Despite what teachers of high school science classes solemnly intone, this business of doing science is the least straightforward endeavor that can possibly be imagined. This was brought home to me in a series of unfortunate events that unfolded this week.

At first, it seemed to be that rare triumph where my simple test of a straightforward prediction actually yielded a clear positive result, instead of the more typical back-to-the-drawing board head-scratcher. If this were a story, that the protagonist was a protein named Diaphanous could serve as a hint that the plot would not prove as solid as one might hope. (Like many genes first discovered in fruit flies, Diaphanous evokes the appearance of animals lacking a functional version of that protein).

The backstory: Lately, my research has been on how stress fibers remodel to accommodate the movements of migrating cells. But as I work on cells in intact tissues, namely the rind of follicular cells that envelops the developing cluster of cells that give rise to a fruit fly egg, I like to consider the natural experiments that unfold in the course of normal development. For example, these follicle cells migrate for a time, going round and round like hamsters running on a wheel, but then they stop and do other things, like flatten out and secrete the eggshell. They still have stress fibers—these are long contractile bundles of a similar composition to muscle, that help attach the cells to the fibrous surface outside them. But these later-stage stress fibers are much stouter and of somewhat different composition.

I had already established that the stress fibers in the migrating cells depend on an unusual partner, amusingly called DAAM, to form. The more typical protein to help build stress fibers is DAAM’s cousin Diaphanous, but I’d done experiments depleting Diaphanous that clearly showed it was not needed in this case. When I depleted DAAM, though, the stress fibers got really wispy. Oddly enough, I’d noticed that in the much later stages, after the cells stopped migrating, had stress fibers unaffected by loss of DAAM.

So the experiment I wanted to do next was to deplete Diaphanous in the later stages. This was not completely straightforward to execute, though, because I had to avoid depleting it too early. I’d already seen that this caused cells to have trouble with their normal round of cell divisions. It’s a common problem in this sort of work that it can be harder to study later processes if you mess things up before they have begun to happen. The solution makes use of the dazzling array of tissue-specific drivers of gene expression that have been invented for fruit flies. They allow you to drive expression of a gene at specific times and places, targeting particular processes you want to study. To keep a gene from being expressed, you can use something called RNAi, which basically makes a cell chop up the instructions for making a protein sent from the DNA so that protein does not get produced.

In short, I needed a driver that acted late in the follicle cells but not early. Our lab did not have such a driver, since we study the earlier stages. But we’d read a paper with some very clever experiments that made use of just such a late driver, one called Cy2. We requested the fly stock from one of the paper’s authors and she promptly mailed it off to us. Fly researchers are awesomely generous. It’s a tradition that goes back to the earliest days of the field over a century ago to share reagents this way.

Chapter the First: Quarantine. The flies arrived and had to be put in quarantine, out of an abundance of caution concerning the possible introduction of mites into our hundreds of lab stocks. In practice, this consists of isolating the vials on the top of the lab refrigerator. All stocks that arrive from elsewhere must be taken through quarantine, save those from the renowned and very reliably mite-free Bloomington stock center. It meant a delay to the start of my planned experiment, until I could obtain 3rd instar larvae and wash them, a rather amusing exercise on which I have previously posted.

So there the flies sat, two healthy vials with clearly written labels: Cy2/(Cyo); Dr/TM6b. This cryptic shorthand conveyed that along with the driver I’d asked for, the flies conveniently included markers on another chromosome, in case I wanted to build more things into the stock. Annoyingly, they were all senescent adults and developing pupal cases—ideal for surviving the mailing process, but the worst possible stage of colony development for obtaining sufficient larvae for my purposes. I would have to wait several weeks for the new generation to produce larvae I could wash.

In pre-covid times, I could have done the cross right away with existing males, dissecting the offspring on a quarantine-use microscope belonging to a neighboring lab. Normally we share a lot of equipment freely in our department. But the physical distancing requirements have temporarily stopped that sort of thing. And we can’t risk getting mites onto the equipment we use for all our normal work.

To shorten the waiting time (a frequent concern of fruit fly researchers, especially I would think those of us who work on adult rather than embryonic or larval structures, meaning our crosses must extend to the full 10+ days of development time beyond any stock-building that precedes it), I planned to wash enough larvae to siphon off a number of males for the experimental cross. To that end, I also began “blowing up” the stocks I would obtain the females from; I could virgin them ahead of time and have them all ready to go as soon as their husbands emerged from their pupal cases.

When you’re waiting to wash a quarantine stock, impatient for the experiment to begin, they seem to take longer to develop, much like a watched pot. The stock contained the mutation Tubby, which makes for shorter flies but a longer developmental time, so that was part of it. Also room temperature (on top of the fridge) slows development compared to the flies’ optimal temperature of 25 C (that’s 77 to your Fahrenheiters...and to be honest, most of us American scientists are very compartmentalized in their understanding of Celsius; outside of the lab context we speak it no better than the average U.S. citizen). So far, then, the slowness makes sense both physical and psychological. But why the quarantined flies should always produce their burst of 3rd instar larvae on a weekend day, and on the one weekend day I don’t pop into the lab, is more puzzling. But it is the rule, I have found.

I wasn’t going to let it happen this time. I watched them like a hawk (a mosquito hawk?) and sure enough, it was a Sunday when all the larvae began to wander. Wandering larvae is the other, more romantic name for the 3rd instar of Drosophila melanogaster, because they have at last eaten their fill of the mushy rotten fruit they have been burrowing through, and there is nothing else for them to do but come out into the light and air and begin to claim their inheritance as winged creatures of the sky. First, though, they must choose a spot in which to prepare their new bodies. Here in that lab, they climb around on the clean walls of the vial, above the caramel-colored dollop of food, fat, juicy larvae as big as a good-sized grain of rice, big enough to grasp gently in forceps and take through the three ritual baths, soapy water, ethanol, and salty water, that remove any lurking mites or mite eggs from their surfaces. After being placed in a fresh vial and wicked dry with a twist of Kimwipe (lab Kleenex), they will crawl around a bit more, mingling with their certified-mite-free compatriots. In a few more hours they will settle down, stop moving, and let their skins harden into bark. Inside that bark, they pretty much dissolve themselves, save for a few set-aside clusters of cells. They go on to rebuild their bodies into the adult form, complete with intricate jointed legs and multitudinously-faceted eyes and iridescent, cellophane-like wings over the course of about a week (at room temperature).

I spent several hours washing more larvae than usual to establish a clean stock, wanting to have plenty of extra males to father the experimental crosses. If I’d had access to the quarantine microscope, I could have selected extra male larvae—you can already distinguish males and females at this stage-- but it would not really have saved time. I played the numbers game instead. It was a Sunday afternoon, quietest time of the week in lab, and very peaceful. I took my time and changed the bath solutions often to make sure there wasn’t too much soapy water in the ethanol or too much ethanol in the final rinse. I wanted this all to go smoothly with no delays.

I put the now-lawful vial in the 25C incubator to develop, after carefully copying the genotype from the original handwritten labels: Cy2/(Cyo); Dr/TM6b. Incidentally, there are lots of markers of chromosomes, many going back to the original mutations described by early fly workers such as Calvin Bridges and Alfred Sturtevant. They let you follow with visible traits the invisible genes that you wish to follow through the generations. Various labs have their favorite markers, but some such as Cyo (which makes for curly wings) are ubiquitous, and Dr and TM6b were familiar to me as well. Dr (short for Dropped, I don’t know why) makes the eyes very slitted, and TM6b is a whole set of markers that comprises what is called a balancer chromosome: a chromosome that has been scrambled and rearranged so that even though it still has all its genes, they are in the wrong places. This means that none of the usual recombination between sister chromosomes that occurs when egg and sperm form can happen. The advantage to the researcher is that this keeps genes segregated in predictable places. Otherwise, all those markers would not be reliable indicators letting you keep track of the genes you put in place from one generation to another. TM6b can actually include various different markers, but one of them is Tb, easy to recognize in both the shorter larvae and pupal cases and to some extent discernible in adults as well.

Chapter the Second: Cross Purposes. Fast forward two weeks (you can—I sadly could not—this being November of 2020, I would certainly have appreciated the distraction). So I waited, none too patiently, for the new adults to emerge. Meanwhile, I tended the stocks I would virgin for females: two different RNAi lines for Diaphanous and one, a control, for its cousin DAAM which I already knew was not required for the later-stage stress fibers. I built up a collection of ladies in waiting, captured shortly after their eclosion and isolated in vials away from all male contact, so I could be sure their offspring would be the genotype I wanted. [A note about the term ‘eclosion’: one might be tempted to call the emergence of the adults from their pupal cases ‘hatching’, but that term is reserved for the larvae coming out their eggshell. You only hatch once, even in the doubled lifestyle of these metamorphosing beasties.]

Finally the washed flies began to eclose. All my usable Cy2 flies were in that one vial. I briefly knocked them out with carbon dioxide gas, used a fine paintbrush to separate the males, and added 3 males each to the three bevvies of expectant females. There were still a few males left, enough to establish the new stock of Cy2 for future use.

At last, more than a month after conceiving it, I’d begun the experimental cross. It would be two more weeks before I had the flies to dissect and the beginnings of an answer. Fly work involves a lot of waiting, and to cope with that we tend to have a lot of irons in the fire. All that juggling can be rather distracting. Sometimes, depending on how other experiments have gone in the interim, I’ve unfortunately moved on from the original urgency of a question by the time the flies are ready to examine. It’s a hazard of the work.

Though I did not yet realize it, I’d made two mistakes. First, I should have looked a bit more carefully at those Cy flies. Second, I should have done the proper control. Sure, crossing them to the DAAM flies was a pretty good control, but there was an even stricter one, that tested whether the driver stock alone had any effect (it should not, but you like to be sure). I should have crossed the Cy2 flies to what we call wild-type, a stock called w1118 that has white eyes, incidentally [link] the first fly mutant ever identified and the foundation of fly genetics.

I hadn’t wanted to use up any more of my precious males, and figured I could always do that control later, if the experiment turned out promising. A lot of us cut corners that way, and it isn’t necessarily less efficient. But sometimes it snarls you up and wastes your time instead of saving it, and makes you go through all sorts of contortions trying to make sense of your data with less information than you should have had.

Chapter the Third: The Experiment. I waited out that two weeks, pursuing other work and trying not to pay too much attention to the news. I wore my mask and stayed in touch with my loved ones over zoom and the like. I hung up bird feeders to entertain my cats and my family alike. I went on long walks by the lake. Time passed. At last the grand day arrived: my experimental flies had begun to eclose. I gassed them and tapped them out of the CO2 pad. Now here was a wrinkle I’d shoved to the back of my mind: those extra markers that I didn’t need, the Dr and TM6b. In a clean experiment I’d have gotten rid of them, but that would have required another couple generations. I’d wanted a quick provisional answer, in order to decide whether it was worth the time and trouble to do the more careful version of the experiment. So: would I dissect the TM6b-carrying flies, or the Dr-carrying flies? It had to be one or the other. The balancer chromosome carries a number of mutations so it would be more likely to do something weird to the cells I was interested in. Not that that was very likely, but I might as well be careful. Dr it was then: that only affected the eyes, as far as I knew. What were the chances it would mess up my experiment on stress fibers in follicle cells?

But none of the flies had Dr eyes. That was odd. I looked closer. Half of them sure looked like Tb flies, shorter and a bit chubbier, though you never want to depend on your ability to discern that marker in adults. The others, the longer ones? They did have some oddly short hairs on their dorsal thorax (around the back of the lower neck, if you want to be anthropomorphic about it), much shorter than the clipped ones you see with the marker Stubble. It kind of reminded me of a marker I’d seen once or twice. Well, that must be what these were; maybe the label had been written wrong.

Impatient to get the experiment done, I swept the short-haired flies into a fresh vial with a bit of yeast. The yeast was to encourage egg production (they’re called fruit flies or vinegar flies, but it’s really the yeast on the rotting fruit that they’re after). I added a few males which were there for the same end. You could say the way to a fine set of ovaries is through both the heart and the stomach. Two more days to go before the dissection. For good measure I put some plain-vanilla w1118 flies on yeast to serve as extra controls.

On the appointed day, I got out my fiercely pointed #55 forceps and began the dissection. I nearly messed up by dissecting the early stages by habit—the technique to do so destroys most of the older egg chambers—but luckily remembered what I was about it time, and switched to the method to optimize acquisition of undamaged later stages. I fixed for 15 minutes in 4% paraformaldehyde, rinsed three times in phosphate-buffered saline solution with Triton-X detergent, and added a stain that would label the filamentous actin, the principle component of stress fibers among many other cellular structures. I put it in the lab fridge (the one where no food is allowed!) to stain overnight. The next morning, early, I came in and rinsed off the stain and made slides. Then I went to the womb-like room where one of my favorite workhouse microscope lives, the renowned Nikon 800 laser scanning confocal microscope. I did the necessary 2020 ritual wipe-down of all surfaces with 70% ethanol, and fired her up.

And oh, it was beautiful. I was so disciplined; I began with the controls to set up the correct laser intensity and gain at which to collect all the images, so the brighter ones would not be out of the range of measurable brightness and everything could be properly quantified. But it was already clear from the what I saw on the computer screen as I centered examples, focused, and took images that the experimental egg chambers had strongly reduced stress fibers. I took lots of pictures, happy that for once my experiment had gone as planned and given me a clear answer.

Also, can I just say how much I love the stain Oregon Green phalloidin? The name itself is lovely: as a native of the Pacific northwest I find it so evocative: the green of deep cushiony moss and ferns and forests of hemlock and douglas firs; and phalloidin itself is a stain derived from mushrooms with which those forests are rife. (Phalloidin, now there’s a scary toxin: it binds so tightly to filamentous actin that it stops your heart. Unlike a lot of other toxins, it doesn’t make you nauseated, so you absorb it until it’s too late for any antidote. But that’s why it’s such a good stain. You just have to wear gloves, or wash your hands after pipetting it. And we all wash our hands so often nowadays it makes no never mind.) There’s red phalloidin, and far-red phalloidin, and even ultraviolet phalloidin (but most microscopes don’t have the right filter sets to light it up very well): but green phalloidin is the king as far as I’m concerned. So bright, and a short enough wavelength (only 488 nanometers, vs. 566 or 647) that it shows up structures the more finely. You can definitely see the difference: it’s sharp as can be.

So, I had the preliminary results I had hoped for: the Diaphanous flies had reduced stress fibers. It doesn’t actually happen to me all that often, that I get a clear answer, either what I predicted or the opposite which is almost as good in science. At least that’s progress, an increase in understanding. No, usually I stumble over these head-scratchers of outcomes. Interesting results, but interesting in a complicated way that require a lot more work to make sense of, if you ever do. It’s partly down to most of my experiments involving imaging with a microscope: you get a lot of unexpected information that way, if you keep your eyes open. But it’s also that I seem to be attracted to the sort of problem that does not yield neat answers—the way some people are attracted to overly hairy guys on motorcycles who are a bit too into mild-altering substances and petty crime. I think I’m the one to straighten them out, but usually I’m the one who gets burned. But this time I had prevailed!

This was just a start; of course I needed to replicate, do some more dissections, get more numbers, reach levels of statistical unassailibility. In particular, I didn’t have as many clear examples of the DAAM control as I needed. Also, I’d do the proper control, and maybe even un-double-balance that Cy2 stock to get rid of the pesky extra markers.

Chapter the Fourth: The morning after. Yeah, and now I’d better take the time to figure out what is going on with that marker that is not Dr. Because, unlikely as it was, wouldn’t it be a shame if it were somehow affecting my results? Worst-case scenario—because that’s how we self-questioning scientists have to operate, ever since the dawn of time or at least the Enlightenment—worst-case scenario, then, is this marker, whatever it is, is the thing responsible for the reduction in stress fibers. Oh, but that’s very unlikely, I tell myself. Besides, the DAAM controls didn’t have reduced stress fibers.

I looked at the original handwritten label, still on the vial of flies on top of the fridge in quarantine. Maybe that D might actually be a P. What was Pr? I’d never heard of it.

I went to the master compendium of fruit fly genetics, FlyBase.org, and looked up Pr. Purple, an eye color gene on the first chromosome. I was looking for a gene on the third chromosome, so that couldn’t be it. I tried a different approach: I DuckDuckWent (DuckDuckGoed doesn’t sound right; if you haven’t heard of it, it’s a more private alternative to Google) images of Drosophila markers. There was that classic poster I’ve seen hanging in various labs, of the most common markers. And there was that marker I’d been reminded of, with the very short hairs. Sn it was called. Could that be my marker? It would have to be some pretty bad handwriting, to make an S look like a D; r to n is easier to imagine.

I went back to FlyBase and looked up Sn. It was the gene Singed. Like if you got to close to the outdoor fire pit on the patio (a way to safely hang out with your friends outdoors even during the Chicago winter), and singed your eyebrows most of the way off (and no, I haven’t done that yet). Also on the first chromosome, though. But look here, this is interesting: Singed is an actin-bundling protein. I read further down the page that summarized the work of dozens or hundreds of researchers over the decades. Yes, it was expressed in the ovaries, and yes, it was known to affect stress fibers. That would be worrying if it were my marker. Lucky it’s not.

I wasn’t getting anywhere. I tried yet another method, going to the webpage for the Bloomington stock center. It’s very well organized, and they have a page showing the details of all the balancer stocks they keep. There ought to be a clue here, for any marker that a researcher could assume another lab would recognize. I go down the list to the TM6b stocks, and find it. Pri, aka Pr, for Prickly. Causes short thoracic bristles. That’s my guy.

Back on FlyBase, I learn that Prickly is one of the classic mutants discovered in the early days of fly research. And this is weird: it has not been annotated. That is, nobody has figured out what gene it is a mutation of, let alone what biological processes it participates in or what tissues it’s expressed in (this matters because if it’s not active in the follicle cells, my experiment would still be valid). They could; it’s a straightforward enough task given that the whole genome is sequenced, but apparently it’s not one that anyone’s found worthwhile. So all we know is it makes very short, deformed bristles that look to me a lot like those of Sn.

Okay, now I am getting worried. What are the chances that this is NOT a protein that affects something like actin bundling and therefore messes up stress fibers? Maybe I had only seen what I wanted to see with the DAAM control. That’s a hazard of doing science, because it’s a hazard of being human. That’s why controls are so important. I consider my experiment in this new and harsher light. Maybe the Diaphanous results are just a phantom of wish fulfillment, summoned by this Prickly hitchhiker I’d never meant to take along for the ride.

I’d already begun the proper control that would answer this question, but meanwhile, while I wait for those flies to emerge, is there anything else I can do? Maybe I should dissect those formerly scorned Tubby flies; at least they lack Prickly. But according to the list at Bloomington, that particular stock has a number of other mutations on its TM6b chromosome, including one called Bri. Bri is a twin of Pri in more ways than one: it also causes very short bristles, and is also unannotated so we have no idea what protein it makes or when or where it acts in the body. Without asking the researchers who sent me the flies, I had no way of knowing if Bri was in there or not.

It would be a bit awkward quizzing them about their flies. We all tend to overdo the shorthand in labeling our stocks, and don’t always remember all the extra mutations lurking there. It’s tripped me up before, when I uncovered interacting mutations I hadn’t known to worry about until they unhinged my crosses. Don’t get me started on vermillian eye color: it’s a real bear. Either way, I’d have to check the controls and unbalance the stock to have a real answer, so probably better not to pester them.

I can’t resist having a quick peek at the TM6b flies though; I’ll be dissecting them tomorrow and should know by Sunday or Monday if the Diaphanous results are evaporating or not...that is, if Bri or something else is not further muddying the waters. A positive result would be definitive; a negative one will require further research. Well, either one will require further research, but one will be more cheerful and the other more like putting nails in a coffin of my hopes one more time. And that, my friends, is what it’s like to do science. (At least I get to see more Oregon green on the confocal, though).

Epilogue. What lessons can we draw from this (mis)adventure, this stomach-churning roller coaster ride of thrills and doubts that is my life in science?

1. Do the proper controls from the beginning. (Although that would have cut out the thrills as well as the doubts, so to be honest, I’m not totally on board with this one).

2. Take the time to look at the flies you are about to cross, and make sure they have the markers you expect. Harder, probably unrealistically hard, is to make sure they don’t have the markers you don’t expect. That would require a Rumsfeldian level of perceiving unknowns unknowns.

3. Remember the limitations of shorthand for conveying a genotype, which like the face we present to the world is invariably far more complex than there is room enough and time to write out.

4. Murphy’s law reigns supreme in this world of ours. What were the chances that the unwanted marker I’d thought I could ignore for a first-pass experiment would turn out to be a different marker I’d never heard of that might affect stress fibers in my cells? Still, it made for a good story, which I haven’t come across in all this interminable slog of an Autumn.

#drosophila #science #research #lab work

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sibyl-of-space · 4 years ago

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havent angsty long-posted on here in a while lol it’s like i am in 3rd year of college all over again

Well, no thanks to fast and reliable testing, I am at least fairly sure I actually do have Covid. I still haven’t gotten my test results and it’s been a week, but I have a headache typing this because I over exerted myself by performing the highly taxing tasks of... taking a shower, cooking something, and sitting upright instead of lying in bed (as I have been largely doing for the last 2 weeks). So at the very least I’ve been able to communicate with my workplace and am now at an understanding that I should just take this week off too because if sitting upright for extended periods of time makes it hard to breathe, that is perhaps, slightly concerning.

So instead my anxieties now are about the fact that literally contracting Covid was actually in the long run beneficial for my mental health because I’ve been able to just... rest, and play some video games, and rest some more, and sleep, and edit photos, and watch other people play video games. I’ve gotten to just. Relax. I haven’t had the chance to relax since before the pandemic started, because I work at a nonprofit that helps homeless people and that shit doesn’t stop being necessary in a crisis. And it’s compounded by the fact that I work in IT and we had to basically throw together enough band-aid and duct tape solutions to get everyone set up to work remotely, which was hard as fuck to do, and now we are still going in part of the week to help serve meals shifts because we don’t have any volunteers.

(That is like 90% definitely how I contracted it, a few weeks ago I worked a meals shift with someone who was not-so-subtly coughing into his mask next to me and it was not the phlegm-y cough but the ‘ah i am just clearing the old airways’ cough. Why are you working a meals shift to serve the homeless if you have symptoms OH MY GOD. Anyway.)

My workplace is not organized in the best of times, which made it interesting but lively and was largely endearing even if it was somewhat frustrating. But now, it is just driving me up the wall. I have felt at the end of my rope mentally for about 2 straight months with no end in sight. And now the more literature that comes out indicates it could be YEARS before the USA is able to approach anything resembling normal because of this, and I do not think I am mentally capable of handling this kind of environment for that long.

My team is GREAT. I love them. This organization put out the only legitimate IT starting position that did not require 3-5 years of previous experience, paid me MORE than a fair starting wage, and is just all around great at treating me like a person and I’ve learned so much from them. I am so thankful. But the organization as a whole is managed like, well, basically a shit show that makes me honestly miss the well-oiled shitty corporate bureaucracy that was my first job out of college. I’ve been considering applying elsewhere for ages now, but... well first of all I feel like I need to at least have worked a full year here before moving on (it’s been about 7 months presently) for it to count for anything, and also, well it has been very nice actually being treated like a person in a 9-to-5:30 office position and I don’t know how much I’ll be seeing that if I sell out and work for some tech startup or whatever the hell.

Up until now I have had a sort of... promise I’ve kept to myself, where if I am not fulfilled and start to actively dislike and resent my job when I wake up for it in the morning, that I will move on from that job. I have held on to that. My first job out of college was an excellent source of stability for me as a recent college grad, but when it reached a point it was stressing me more than it was supporting me, I moved on. I then worked minimum wage (note: minimum wage was $15/hr at this point so my income was only actually decreasing by $4/hour because administrative assistants do not get paid what they are worth. Now bay area cost of living is fucking stupid, but minimum wage in this circumstance was actually livable for me with the bonus of having my parents’ health insurance.) at a fabric store - I stayed there for 2 years, longer than I’ve ever held a position, and while partly I left because I had more or less finished learning what I had hoped to learn from my coworkers, the store was transforming away from that kind of community DIY vibe it started out as and turned into a kind of etsy wine mom vibe that didn’t click with me as much anymore, and also I was turning 26 and would lose my health insurance through my parents.

So I took the next several months taking my first real break from constant responsibilities/employment since pretty much ever, focused on speedrunning Tales of Symphonia and eventually started studying for a baseline IT certification so I could try and get a job that was more appealing to me than administrative bullshit, which I hated so much. My closest friends I made in my first corporate job out of college were people in QA and IT, so I decided to try and make that a reality. I studied my ass off for a couple solid months, took both tests for the A+ certification, passed, and started applying to jobs. And now here we are.

My frustration stems from the fact that I want to continue working with the people who took a chance on me when I was very green and had no experience, just 2 tests under my belt and a willingness to learn. I really do want to continue learning and growing here. But the environment, through no fault of anyone’s, has been just grating on me. I honestly believe that if I quit they would all completely understand, but I don’t WANT to - I want to stick it out, partly out of pride (this organization has such high turnover, I have already outlasted over half of the new users I’ve onboarded.....), partly to repay the team that has invested in me and treated me like a human being, and partly because I know more experience is necessary if I want to land another job anywhere else.

But boy, am I drained. I was literally hoping I had Covid because it meant I could get 2 weeks off work. And here I am, finally got Covid, am taking 2 weeks off work, and yet I still feel bad about it because a damn test hasn’t come back making me feel validated about it and I’ve still partially convinced myself I’m being a drama queen.

And this break from work is just making me dread the prospect of YEARS of this kind of uncertainty. It’s been so nice just kind of being able to do things at my pace, but it took me actually contracting The Plague to actually get this kind of relief.

Anyway, a few bright sides... first, my tortoise is adorable and doing great, so there is that. (Just looked over and saw her doing something cute. I love her.) Second, I will continue to rest this full week, and next week there is a good chance I will feel more empowered to tackle a workday with the perspective of fresh challenges as opposed to just some monotonous daily grind, after having this time off. Third, if I continue to feel nothing but dread and frustration regarding work, I think I should stick to that promise to myself and give a deadline to when it’s time to start looking elsewhere and move on, because no organization is worth my mental well-being. And lastly, I’ve re-visited OoT for the first time in a really long time (handhelds are about all I can do at the moment), and that was really special.

I’m still fucking pissed that there is pretty much no chance I’ll be able to go to France this Christmas with my roommate and girlfriend. I was so excited to have a paycheck where I could afford to do something not just for myself but for the people closest to me, I routed our flights so we had layovers that would let us connect on the main flight together and paid extra so we could sit together, I was SO excited about this because I spent almost EVERY Christmas in France as a kid because of my family and I miss it SO MUCH, I was so so so excited to share these intimate memories with people I really care about, but the USA is a fucking dumpster fire shit show so who knows when I’ll be able to actually do that now. I haven’t cancelled the flights yet but I’m coming to terms with the fact I’m going to have to do so. Hopefully I can get a refund and we will just have to go next Christmas, but I’m still really fucking upset.

Not even going to comment on how much of a shit show the USA is in general. I feel completely helpless. Another 4 years of Trump is literally a catastrophe we can NOT permit but the current DNC platform looks like it is going to fix about, oh, FUCK ALL, so at this point it feels like there is no hope unless we literally start busting out the guillotines. Every time I see an Elon Musk fanboy on Twitter some of my remaining faith in humanity crumbles. Then I think about how people have been protesting EVERY SINGLE DAY for the right of Black people to LIVE and NOTHING is being done about it. It’s both exhausting and terrifying.

In conclusion, I would like to stop living through a major historical event, please and thank you.

#this is very stream of consciousness #personal

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