#like overstirring it + one of my samples leaked which is soooo embarrassing bc ive already had to ask for more before bc its taken me-
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this assay is so fucking fake......
#same one ive been working on for like 3 months. every other assay ive trained on took me a couple goes to get but ive done this one ~45x#and i keep getting 2 good runs and then 1 fail. which SUCKS bc i need 3 passes in a row to sign off on it#and its so sensitive that changing even tiny things like using a different brand same volume beaker. or a 0.5cm longer flea#anyway i had another 2 good runs this week so this was my 3rd but bc its a friday afternoon im tired as fuck and keep making dumb mistakes#like overstirring it + one of my samples leaked which is soooo embarrassing bc ive already had to ask for more before bc its taken me-#almost 50 fucking attempts already#anyway. hour and a half into prep and im at the most crucial time sensitive part which is pipetting thr enzyme into the substrate#and i manage to do it all w even time spacing (u have to replicate the exact same pace at the end of the timer or it doesnt work)#and then realise id picked up a different identical model pipette that was set to half the volume i was meant to put in FUUUUCK#by that point i was like fuck it im almost 2 hours in and nothing else to do the rest of the day. so ill work around it + see what happens#i figured well its half the volume. so if i add the same half volume again at the 5 minute mark and leave it for 12.5 instead of 10 mins#then itll hydrolyse the substrate to the same degree. IN THEORY in practice this stuff never works bc of error margins etc#bearing in mind this js like 30 seconds of thought bc it took me a couple mins to realise what i did#but the thing abt working in a lab is u make these split second decisions constantly bc everything is so time sensitive#so u have to be quick thinking on ur feet#anyway long story short got to the end of the 3 hour process. which i was carrying out v sloppily bc the chances of it working were-#slim by that point lmao. but lo and behold it was completely fucking fine. all cvs less than 5% and averages <5% of spec#which is awesome bc it means after THREE MONTHS and like. 45x3 whats that AT LEAST 135 HOURS OF FOCUSED TIME ON IT#not counting attempts i gave up on halfway thru bc id alreaady fucked them up bad#i can FINALLY sign off on it lmfao. but im just so mad like why does it play these mind games with me. it shouldnt have worked#whatever chemistry is such a fickle stupid science. anyway wahoo weekend time baby#gorgeous weather here + im gonna get pizza on the way home...... maybe life doesnt suck sometimes 😇#mutuals if ur still at work stay strong soldiers#.diaries
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