#blooddetectionmethods

[forensicfield]

Formulations for Chemical Solutions

1.) Ninhydrin (Kent, 1998; Champod et al., 2004, p 239)

Stock Solution: 25 g ninhydrin dissolved in 225 mL absolute ethanol, 10 mL ethyl acetate, 25 mL glacial acetic acid.

Working Solution: 52 mL of stock solution diluted to 1000 mL with HFE 7100.

2.) Zinc Chloride Solution (Champod et al., 2004, p 240)

Stock Solution: 8 g zinc chloride dissolved in 180 mL ethanol, 20 mL glacial acetic acid.

Working Solution: 6 mL of stock solution diluted to 100 mL with carrier solvent (e.g., HFE 7100).

3.) 1,8-Diazafluoren-9-one (DFO) (Kent, 1998; Champod et al., 2004, p 230)

0.25 g of DFO dissolved in 30 mL methanol and 20 mL glacial acetic acid. Add this to 725 mL HFE 7100 and 275 mL HFE 71DE.

4.) Nonpolar Ninhydrin (Stimac, 2003a, pp 185–197)

Stock Solution: 1.5 g ninhydrin dissolved in 100 mL HFE 71IPA (may require refluxing at low temperature).

Working Solution: 15 mL of stock solution diluted with 100 mL HFE 7100.

5.) 1,2-Indanedione

0.25 g 1,2-indanedione dissolved in 90 mL ethyl acetate and 10 mL glacial acetic acid. Add this to 1 L of HFE 7100 (Merrick et al., 2002, pp 595–605). or 2 g 1,2-indanedione dissolved in 70 mL ethyl acetate. Add this to 1 L HFE 7100 (Almog et al., 1999, pp 114–118).

6.) 5-Methylthioninhydrin (5-MTN) (Wallace-Kunkel et al., 2006, pp 4–13)

1.7 g 5-MTN dissolved in 52.5 mL ethyl acetate, 50 mL methyl tert-butyl ether, 12.5 mL absolute ethanol, and 5 mL glacial acetic acid. Add this to 360 mL HFE 7100.

7.) 2-Isononylninhydrin (INON, Thermanin) (Al Mandhri and Khanmy-Vital, 2005)

4–5 g INON dissolved in 15 mL ethyl acetate, 5 mL isopropanol, and 980 mL HFE 7100.

8.) Silver Nitrate (Trozzi et al., 2000, pp 38–39)

Dissolve 30 g silver nitrate in 1 L distilled water. or Dissolve 30 g silver nitrate in 100 mL distilled water and add to 1 L ethanol.