#and secondly. i can't figure out where the positions function is in ZenBlue. and the scope core staff only work for a random three hours of

[unopenablebox]

Holy God This Is All So Boring

i am taking microscope images of the cells i'm studying. the cells were grown on a glass plate before i fixed them (killed & chemically preserved), so by default a microscope image of them is taken from a camera below them, looking up through the glass. they're stained with fluorescent dyes for four different proteins, so every single picture has to be repeated four times with a different laser light illuminating the cells (imagine taking a photo with a red filter, a blue filter, and a green filter, and then composing them all together to get the full picture. it's actually almost exactly the opposite of that, but that's close enough).

i care mostly about how the cells are shaped in three dimensions, and i'm using a laser which is specially shaped so it can collect only a very thin slice of the cells in the Z-direction, without interference from the parts of the cells just above or just below what i'm taking pictures of. as a result, i need to take lots of pictures at different depths in the cells, so i can get slices that i can stack on top of each other and get back a 3D shape. also, because i am using a tiny concentrated beam of light to achieve the above effects, it has to scan across the image to collect each picture, like a scanner; it can't just be collected in a single snapshot like a photo.

the distance between one slice and the next is less than a quarter of a micrometer. i'm using a 63x magnified magnifying lens to magnify the image, and the light detector that picks up the light is specially made to allow the images to be processed even further, so i can resolve structures that are less than 200 nanometers, which is the Abbé limit and is the technical resolution limit of light microscopy (don't worry about this). i care about things that are the size of, like, three proteins stuck together, and therefore maybe 10nm wide, so this is important to me.

all of this is, you know, scientifically great, very useful to me, i'm getting some very interesting results that i am genuinely looking forward to thinking about more, except the upshot of all of this is that just getting a single picture of two cells from the bottom to top of the cells involves 80-100 slices and takes like 27 minutes per image to collect, and i need at least six pictures tonight, and certain bastards in certain other labs habitually pre-book the microscope so i can't use it except at 5-9pm on a friday. no one else is here in the lab and my mother is busy with elder care and my girlfriend is busy with like, groceries, so i can't call either of them even if i weren't too irritable to be good company, and oh my god, i am so bored, i am so so bored, i am bored enough even to type out this whole explanation even though none of you could possibly care because it took most of my current round of waiting for 27 minutes to do