#TGC MFS

[infoglitch]

(I bet y'all mfs don't remember when I created rouge for the rose reaper and dork knight series did ya? Well guess what-)

Dork Knights Rose Remorse!

I'll finish this angst war with a critical blow to you, @epic-arc this is and always will be the glitch cycle!

Jaune sat alone, his appearance much older as he appeared in his 60's as he sat in a chair on his cabins porch, looking at a field of Roses and sunflowers.

A young man walked up to him, his hair black, eyes azure blue, wearing a red t-shirt and black jacket as well as white jeans. He sat beside Jaune as the now old huntsmen smiled.

Rouge: the garden looks good.. I'm surprised you planted the roses so close.

Jaune: well.. your aunt and mother were always close..

Rouge: yeah.. how is Aunt Blake doing?

Jaune: we talked.. she's on the end of her rope.. I worry for her

Rouge: you worry for everyone old man.. so.. are we gonna talk about mom?

Jaune: Rouge.. I know it's been awhile-

Rouge: dad! It's been months.. you need someone to talk to. And I know you haven't talked to Aunt Blake or Mrs. Schnee.. you can't bottle up your grief!

Jaune: I'm not! I just.. your mother was.. she was my everything.. she made every day feel like I could live.. she inspired me after Beacons' fall.

Rouge: that doesn't mean just because she's gone you should shut everyone out.. when have you last talked to great uncle Qrow? When have you talked to Mr. Pine?!

Jaune:.. I don't want to discuss this anymore.

Rouge: she was your wife, but she was my mother.. I know how much it hurts.. or at least I can try! We both know mom would kick out asses for mopping!

Jaune: I said-

Rouge: how long until you realize you can't just shut people out dad?! How long until you realize you're shutting me out?!

The air went still as jaune sat there. He would look at his son as for a moment he could see both himself and his wife in his son's eyes.

Jaune:.. it just... It hurts.. it hurts because she doesn't get to be here, because she had to go on that mission, that one mission to destroy the relics! It.. it isn't fair, she was 55!

Rouge: and I was 16 dad. 16. It wasn't fair I lost my mother and it wasn't fair you lost your wife.. but im not about to let her death ruin my family.. your 61 dad, she died 4 years ago... You need to move on.

Jaune:.. I miss her.. so much.

Rouge sighed as he placed a hand on his father's shoulder.

Rouge: i miss her too dad.. I miss her too.

[nyikondlovu]

You assume they, at 8 and 10 each, knew how Rhaenys bonded with Meleys.

Baela, Jace and Luke who had Moondancer, Vermax and Arrax hatch in their cribs respectively and Rhaena who had the egg that never hatched.

You assume, that at 8 and 10, the girls would easily get over being told they are pigs and that they were wrong to mourn their mother instead of chasing a dragon by the cousin they never even fucking met???

And also, Daemon TRIED to bond with Meleys, SHE didn’t want him while Rhaena wasn’t even given the CHANCE to try

Are you f*cking serious? 🤡

You're so stupid! lmfao

Knowing this, these idiots attacked Aemond in a crowd, beat him, cut out his eye and made him disabled. You are such clowns!

🤡🤡🤡🤡🤡🤡

[idealoblivion]

dating isaacwhy headcanons

first things first SIZE DIFFERENCE

that man is bigger than you and he loves it

loves when you have to look up at him

sometimes you’ll just be talking to him and looking up at him and he’s not paying attention to a word you are saying bc you just look so pretty

also he’ll push your hair out of your face (like he did to larry in the grocery shopping vlog) when you are talking if you have hair that falls into your face a lot

loves being able to wrap his body around yours

especially if you are chubby bc youre soft and squishy

big hugger

always wants to be touching you

hand on the thigh

holding hands or having his arm around your waist or on your hip as you walk

you are his passenger princess

you never have to lift a finger in his presence

wanna go somewhere? he’ll drive. want some home cooked food? he’ll make it. need to do laundry? it’s already in the dryer, don’t even worry. headache? he’s got medicine in hand.

he would give you a kidney if you wanted

the boys regularly make fun of him for how down bad he is for you

wants you to wear his clothes, specifically his crew necks

wants you with him while he edits

say you were at tgc house, just sitting on the couch with nick and larry quietly talking with a movie on the the background. isaac comes downstairs and stands behind where youre sat on the couch, hands on your shoulders.

“come sit with me?” he asks looking down at you

“im watching a movie baby” you reply rubbing one of his hands with yours

“please?”

thats all it takes for you to cave and go and sit in his room with him while he edits, occasionally asking you about something to do with the video he’s editing.

once he’s done he will flop onto the bed and partially onto you pulling you as close to him as you could get just to smother you with kisses as you giggle.

clingy mf

would super glue you to him if he could

just wants to be around you

pretty sure i have turned on requests so if you have any feel free to send some!

feedback is always appreciated - c

[stqrgir1e]

smoking with tgc boys !!!

isaac, larry, bigt, yumi, and nick! <3 written while I was genuinely stoned for authenticity. jorge killed it with using the words bro and homeboy way too much 😝

mentions of . . . smoking, drug use, cuss words??? established relationship w/ reader + girl mentioned like once otherwise pretty gn. this might be a bit confusing if your a non-smoker since i wrote for a stoner!reader ( petnames used ➜ hon, babe, baby, pretty girl,)

Isaac ➜ we all know homeboy is more of a drinker. it definitely would be on a dare or bet, he would wanna prove to you he could handle his substances.

"who said i greened out after one joint?... tanner did?... he's lying, we can smoke tonight and ill show you." he would act all smug about it until he actually was face to face with the lit joint.

he would hold it like a cigarette, and when you stifle a laugh at the fact he does so- he would shrug it off while taking a loooong drag of the joint. exhaling before having a small coughing fit. you couldn't help but laugh at his reaction, his face a shade pinker after the humiliating reaction to the joint. but its Isaac- so of course he would brush it off like nothing.

"been a while since I smoked hon... maybe you should finish the rest of it." he would say nonchalantly while passing the joint back to you- insisting you have to finish it. would use the excuse 'he's doing some voiceovers for a few videos tomorrow and can't risk losing his voice.'

def vibes more with edibles. but he always takes half because thats what the back of the packaging says 😡 "why are you taking three! it says half right here!" he would then shove the packaging dramatically in your face- really he didnt want you to see how low his tolerance was and how high he got off half a 15mg edible.

would end up passing out in bed w/ you, gets sleepy especially with edibles/indica. you would mess around and tease him a bit- poking at him while joking how he cannot handle his weed. "nooo, im jus' always down for a nap with you, pretty girl..." he would mumble half asleep, voice drowsy from the high. hella affectionate when high, chronic problem with playing with your hair or like, maybe any bracelets you have on??? i hope this makes sense bro

larry ➜ isn't afraid of the idea of weed, he usually just saves it for social events. like how people are social smokers with cigarettes? larry is like that with weed.

he would get all hyped at a party after seeing you and tanner sharing a one-hitter. "bro! imma need some of that right now." he would act hella shady for comedic effect as well. taking the toke while looking around all mischievously like there was feds hiding in the crowd of the party. you and tanner were stoned- so obviously yall laugh at his antics.

bro is a menace after one hit, isnt even that high he just does it for shits and giggles. lets say nicks filming or some shit- larry is the star of the show. talking his head off in front of the camera while blowing some cart smoke right at the lens. "you see this? its y/n's pen... *takes long hit off cart*... gettin lit tonight." he would definitely hold back his coughs.

speaking of carts, larry is one of those rare mfs that prefer carts over bud. why? he thinks there more convenient- and bud always leaves his nose runny. last time you guys had a smoke sesh date he used up all your sanrio kleenex after smoking 2 bowls 😡

"weed makes my nose run babe! maybe we should've stuck to the cart..." he would say after you complained about him sniffling for the third time. while you were busy explaining why you prefer bud, he would sneakily grab your cart from your hoodie pocket and sneak a few hits. bro is ruthless when it comes to that thing 💀 if you tell him you got the pen on you he's beggin for it.

in fact larry would get a little too comfy with the cart, accidentally almost greening out at a target once when he hit it one too many times in the car. "lets just sit down..." + "on the floor?... in target?..." + "yes babe im telling you just trust me." really thought he was gonna vomit and needed an excuse to sit for a second.

bigt ➜ omg brotha was all over you when you wanted to smoke for your first date!!! he had two little rolling trays set up on his bed prepared for your smoke sesh/movie date.

low-key adorable... literally went out and bought a new one-hitter so you guys would have matching ones, they were green and had little turtles on top of them ): (isnt that so much fun) he wasn't even tryna be cheesy or anything genuinely was just very passionate about smoking. i def feel like him and yumi were smokers in high school so he knows a thing or two abt mary jane. 🤨

"after this bowl i have a gummy we can split..." his tolerance is quite high so he would wanna keep going even after like the fifth bowl. he likes to give his lungs a break though so no carts for him after like the third bowl. edibles from there on out. you know he's gonna be all weird n shit and make you guys split the edible by biting it in half. (like that lady and the tramp shittt bro.)

but at social events and parties??? he's a lot more closed off with smoking- more of a drinker at parties. if he is gonna get high he'll take an edible. "baby weed these days is crazy! I knew a guy who knew a guy who knew guy that got laced with crack!!!" yea he was being sarcastic duhhhh but he was genuinely scared of getting laced at a party.

tanner is a avid cart enthusiast tho!!! he would only use like smoke shop ones though- no street carts for tttt. he would probably prefer zaza's, hes a classic man with taste so he would prefer the cereal milk strain. carts are his go to for when hes just vibing in his room since there pretty much odorless. "I wasn't lying when I said I was smoking zaza!" finds the word zaza hilarious especially when he's stoned.

he takes maaanny tolerance breaks and would probably make you do the same. if you really struggled with staying away from weed he would make t-breaks fun or some shit. (like making a fun little tolerance break sticker chart 😩)

softwilly ➜ nick fr said 🤨 when you asked him to split a bowl with you one random friday night. he eventually agreed because you already had everything set up and he didnt wanna say no after you put all this effort into it ):

"ow! fuck... can you do the lighter babe..." needs help with the lighter, you guys were sat in kind of a awkward position smoking out of his bedroom window since he didnt want any of the guys to question anything 😒 sometimes those flames fight back with the bowls brooo. he has a playlist for smoking after that first time- it kind of becomes a tradition for you guys to try and smoke every friday/weekend (:

his tolerance is worst than isaacs bro. he’s either passed out after the second bowl or laughing at anything that moves. if you guys end up laughing too loud or just making too much noise in general he gets hyper aware about if the boys can hear all the ruckus >:( does not want your smoke sesh to be interrupted. very easily paranoid when high for sure.

he doesn’t love carts- but i mean your his weakness homeboy how can he say no to you every time you ask? “strawberry banana cart?…. fine. one hit, but just to see if it really tastes like strawberry’s and bananas.” it didn’t taste like strawberries and bananas- but he was stoned for the next thirty minutes after that (:

hates the smell of weed. he always has the windows open, a candle burning, incense burning, anything to diffuse the smell of marijuana. he’ll specifically ask you to blow the smoke towards the window- but sometimes you blow it towards the pillows on his bed… or some plushies even just to get a rise out of him. “fuck babe… now my bedrooms gonna smell like kush for a week.” + “it’s just a little smoke!” he would obviously say it sarcastically, he dgaf where you blow your smoke he just wants to be a pain in the ass for funzies.

another big believer in tolerance breaks- but he dosent even know what the fuck a t-break is. he would just tell you he’s taking a break from weed in general. homeboy is very inexperienced in the smoking department and stayed away from it until he was in his early twenties.

yumi ➜ blake definitely has the highest tolerance out of all of them, but he isn’t a raging stoner. probably prefers weed over alcohol especially at parties- has an occasional joint on the weekends or when he has the time but he’s not stoned 247.

yumi is a classic man, he likes classic things. he prefers bud almost over everything else. he won’t turn down an edible but he despises carts because of the aftertaste they have. “babe that shit taste like potting soil… i’ll just take the extra twenty minutes to roll up.” + “it does not taste like potting soil! it’s supposed to be peanut butter and jelly flavored…” after a bowl or two though… homeboy is loving up on the cart!!!! “damn okay… maybe this shit does taste like pb&j…” better be willing to stop at a smoke shop the next day or have your dealer on speed dial because blake is draining that pen 😩

omg he out of all tgc boys fucking loves little smoke sesh dates. like finding a nice little spot off a hiking trail or just in the woods and rolling up together ): he would make sure to have a playlist and everything just like nick this man is soft for you bro. “alright babe are we feelin’ apricot gelato or blue dream today?” very organized with his weed. he knows his favorite strains and doesn’t venture out farther than the ones he knows he likes.

blake is a whole different personality when high, like he’s still blake but… better? idk how to describe it gahhhhh!!! like he’s more laid back, cusses more frequently, very sarcastic, voice a bit deeper/raspier from smoking. “brotha look over there… that bird is straight chillin’ on that tree branch.” + “brotha?…” doesn’t even realize he’s doing it- you would bring it up afterwards and he always denies it. “babe i can promise you i’ve never talked like that when high, maybe you just think i do because your always stoned when i’m high.” if you ever showed him a video of how he talks when stoned he would become hyper aware of how he acts whenever he’s high 💀

homeboy rolls the best joints- how can he not? somehow they always turn out perfectly cylindrical and no leaf actually ever falls out of the joint. he’s got the magic joint rolling hands, what can he say.

omg don’t even get me started on fucking munchies. i am a chronic victim of binge eating while stoned and i have a gut feeling yumi is too. homeboy can be expected to be covered in cheetos dust if he plans on smoking. it brings out the best and worst in him, the worst being eating copious amounts of food in such a short period of time. “your such a fatty babe,” + “am not! you literally scarfed down three zebra cakes an hour ago…”

[isaacswhy]

getting crossfaded wit tgc⁉️

hear me out…

drabbling about this but this is a weekly occurrence in tgc household. thank you for this idea anon

getting cross'd with isaac is fun because weed makes him the quietest mf on earth but a drink gets him Hyper. crossed isaac is in a purgatory. he doesn't know what he wants but he will have fun with you, even if it's just playing mario kart on the floor and not talking. or at the gas station 3 am getting munchies.

nick gets SOOO passive aggressive and defensive, but strangely affectionate. will lay down on you and bitch at you if you try to bring it up. let him take his time. when he has the munchies he becomes a chef?? like he's in the kitchen cooking a full meal? if you're with him he will cook you a serving. hope it's edible (it's either amazing or dog food)

now tanner. my boy bigt. he is sleepy. crossed tanner is so fucking sleepy. like nick, he gets so lovey and physically affectionate but he does not care if you tell him to fuck off. he will drape himself over you and then fall the fuck asleep. has his munchies stashed in his room already (it's all candy)

blake. yumi. idk when he's crossed he's the most strangely normal. he's a loud drunk but he gets high too often that he seems generally normal, and it mellows out his drunk side. but he will get in on larry's conspiracy theories. big time munchies enjoyer. munchies overlord, though, try to touch his food and he will cut a bitch over it.

larry. good god watch out his crossfaded side is not for the lighthearted. he's loud as shit, but the weed gets him Thinking. he will just spew bullshit and jokes until the sun rises because that mf cannot turn his brain off. good luck! his munchies takes him out to eat, and he WILL bring you. will walk to the nearest cheap restaurant and pretend he's sober (he's so shit at it but they serve him anyways because he buys a Lot and tips Heavily)

[internetscenarios]

been wanting to do this for a while ehhehehhe

im very into madoka magica again you will have to excuse all the gifs

living in tgc house ʚɞ

- okay BASICALLY

- there is never a day where some part of the house isnt messy, whether it be someones room, the kitchen, etc

- so if u like cleaning ur in for a treat 😁

- you will have to deal with constant loud noises like screaming and banging oh my god

- ok besides the negative parts it would be so fun living with them

- if ur having a bad day any of them will cheer u up

- im gonna assume u would be a cc, so ur always in vlogs and maybe popping up in the other guys streams occasionally

- if ur a girliee there is gonna be hate,, but every girl who hangs out with guys get some kind of hate

- god that sounds so stupid but YOU KNOW WHAT I MEAN

- doing anything with them all is super funnn

- going shopping or anywhere is chaos

- your always trying to leave sweet or nice notes on that notepad thing on the fridge but someone always ruins it LOL

- i feel like isaac is just male wife completely, so u two have fun cleaning and doing laundry

- if ur faceless or havent face revealed, u are the photographer for their mf instagram posts

- your camera roll is filled with really bad pictures of all of them

- i feel like you would have late night talks with them just about life, then someone else comes down and ruins it LMAO

- your sleep schedule has gotten ruined since moving in

- especially if u would participate in ltlvc😢

- omg they would go all out for ur birthday

- i feel like tanner would steal one of ur presents, nick and isaac would actually put effort into the gifts, larry would try and yumi would also try but jus ask to get take out food

- they mean well🙏

- ok you are getting jumpscared all the time

- like u are sitting on the couch by yourself and isaac just screams from the other room at you

- scary behaviour

- ok grunk is a college guy now,, but if u were on vc with them all he is so fun

- watching spongebob with him

- car rides are either dead quiet bcs everyone is tired after a day out, or they are screaming song lyrics there is no inbetween

- ive said this with tanner hcs but if u were streaming and went to the bathroom someone would sneak in and start pretending its their stream

- or they would turn ur computer off to annoy you

- you never get alone time, like imagine trying to have a nap and someone needs u to do something stupid jesus christ

- OK BUT BESIDES ALL THE NEGATIVE they are funnyy there isnt a day where they arent making u laugh

RAHH ive been wanting to do this forever omg

also guys,, maybe,, send me requests *wiggles toes together*

[abyssforphantoms]

I know it can be kinda rude to make comparisons (and i kept quiet about this for a long time... more than 2 years)

BUT THEN THAT FREAKING 2 EMBERS THING HAPPENED. And I cannot keep this to myself anymore...

OR ELSE IMMA GOING TO EXPLODE LIKE EDEN

Resh (Eden Elder) be shaped like Elf King (from Amulet) XD

Resh (from that trailer thing)

Elf king

I just can't unsee it ;-;

Granted I been an Amulet fan for about 14 years... ( basically grew up reading those comics), so maybe seeing something that isn't there, or both may have a few similar inspirations, or a coincidence.

Also not accusing TGC of anything!!! but they kinda do have a habit of wearing their inspirations on their sleeves and if Amulet influenced them...

ngl that would make me really happy. :D

But wait, there's more... except it goes into some amulet spoilers, so continue at your own peril

So Elf King is Ikol - a ghostly mf entity thing that is connected to crystals who is the main antagonist of Amulet's story...

LOOK AT 'EM

Also, including images of Emily's (and one image of max's) Amulet

But yeah that symbol reminds me of the season of prophecy symbol.

Granted, it is a simple star shape sooo.... like idk

However, I do remember seeing an image that was "supposedly" was a leaked model of Resh on the official sky discord but can not find it again (tbf idk if it's even real), but if u know what I'm talking about... doesn't that look similar to Ikol a little???? Just saying.

There are also a lot more things I could bring up between both Sky and Amulet that remind me of each other, but it was mainly the Resh/Elf King thing that was a worm in my brain...

Also I don't really feel like that would be best thing for me to do because, at the end of the day, I like both Sky and Amulet for their own merits, and while they may have similar themes, they still go in very different directions.

Granted, if someone wants to continue that conversation... I would be interested.

[bucknastysbabe]

Ew I saw another person not happy w chubby aeg headcanons…yes TGC is slim…but this is fanfic and We want aeg chubby bc we Can

Also F&B and it’s original artwork have him heavyset, obviously some Targ genes going on there. He resembled Viserys who was “plump.”

Not that he wasn’t handsome by any means.

“Lazy and somewhat sulky boy, inclined to gluttony at the table and given to swilling ale and strongwine.”

“Not of a warriors build”

ALLL THE MF DOES IS LAY AROUND EAT AND DRINK WINE N PROB DOES NO WORK WHEN FUCKING AT THE WHOREHOUSE THATS GONNA PUT IT ON YOU AND GENETICS DONT PLAY AROUND

Anyways thanks for coming to my chubby aeg Ted talk

✨Begone, let us chub love✨

This is why we’re unionizing🤣

[lovelykhaleesiii]

mf TGC dresses like a 19th century british orphan except I'M the one saying "pls sir can i have some more"

please the chokehold this ancient man has on me 🥵

[mejcinta]

That girl that asked TGC a ship question and said Rihanna instead of Rhaenyra made me blush with shame. Some of y'all are strong mfs. 😂😂😂😂😂

[infoglitch]

.. I wait for the beef between me, @spahhzy , @epic-arc to continue. So then I may make tragedies for the Jaune ships.

For this, this is THE FUCKIN GLITCH CYCLE!

So bring it on, whoever has the guts to stand on business and face the true king of Rwby jaune ship ANGST. I will gladly show you why I stand as that mothafucker glitch.

But jokes aside if y'all are outta fuel I totally understand. Y'all got lives, I just like the feeling of being a fuckin villain.

[infoglitch]

Well shit. This is a certified face off. Everyone who ain't on JJK timing step the fuck back.

But me? Shit man it's fighting time

BRING IT ON MF'S. As soon as one of you falls this shit about to be The Glitch Cycle. TGC FUCKERS.

LETS FUCKIN GO

Whiteknight kid: and the teacher said I could go to my art class but only after I finished my snack and I told her 'that wasn't fair!' cause I reall-hey!

The child voice startled one Weiss Schnee who, with an empty wine glass in hand, raised her head up from nap she was in.

Weiss: Hmm?

Whiteknight child: You're not listening again...

Weiss: No, I'm listening...art class, right?

Whiteknight kid: I think you should let me walk to school by myself... you've changed since dad d-

Weiss: I'm fine...

Rising up from her chair, Weiss, with a slight wobble in her step, grabbed her coat.

Weiss: It's too dangerous to walk alone besides it's good we spend this time together.

Whiteknight kid: You didn't even wake me up this morning... I had to wake you up. I could have been late.

Weiss: You know I'm not at my best in the morning sweetie, cut me some slack, okay? Even adults make mistakes...

Weiss: I'm going to miss you today...aren't you going to miss me?

Whiteknight kid: Are you gonna remember to pick me up?...

Weiss: Of course I am!

Weiss: I love you!

Whiteknight kid: ...Please don't be late.

...

Weiss sat in front of two graves as rain fell heavy. The raindrops were mixed in with the unending tears, snot, and blood red eyes.

Weiss: I'm sorry I was too late.

[thexoonder]

If I see this mf one more time I'm throwing hands with TGC

[khamgiodau]

PCR CHẨN ĐOÁN BỆNH ALPHA THALASSEMIA (05 ĐỘT BIẾN)

I. NGUYÊN LÝ Phản ứng tổng hợp chuỗi (PCR) là phản ứng cho phép nhân bản chính xác một trình tự ADN quan tâm lên hàng triệu lần. Sử dụng phương pháp PCR thông thường với một cặp mồi đặc trưng chỉ phát hiện được một đột biến. Để phát hiện nhiều hơn một đột biến sẽ phải làm nhiều xét nghiệm PCR. Có rất nhiều loại đột biến gây bệnh anpha Thalassemia. Trong trường hợp này sử dụng kỹ thuật Multiplex PCR là giải pháp tối ưu. Đây là phương pháp sử dụng nhiều cặp mồi trong cùng một phản ứng PCR. Như vậy chỉ cần một phản ứng Multiplex PCR chúng ta đã có thể phát hiện được nhiều đột biến cùng một lúc. II. CHỈ ĐỊNH Sử dụng cho tất cả người bệnh có chẩn đoán Alpha-Thalassemia. III. CHỐNG CHỈ ĐỊNH Không có chống chỉ định. IV. CHUẨN BỊ 1. Người thực hiện Kỹ thuật viên xét nghiệm sinh học phân tử đã được đào tạo. 2. Phương tiện - Hóa chất 2.1. Phương tiện - Máy PCR; - Hệ thống máy điện di, hệ thống đèn cực tím soi gel, hệ thống máy chụp ảnh; - Buồng vô trùng (biology cabinet); - Máy ly tâm tốc độ cao (tốc độ tối đa 14.000 vòng/phút); - Máy vortex; - Các loại pipet 10 µl, 20 µl, 100 µl, 200 µl, 1000 µl; - Đầu côn có màng lọc; - Ống eppendorf 1,5 ml vô trùng, không có enzym nucleaza; - Ống PCR 0,2 ml vô trùng, không có enzym nucleaza; - Tủ lạnh 4-8 0 C, tủ âm sâu -20 0 C; - Găng tay. 174 2.2. Hóa chất - Kit tách ADN thương mại hoặc các hóa chất cần thiết cho tách chiết thủ công ADN (proteinase K, đệm ly giải tế bào, phenol/chloroform, cồn tuyệt đối). - Hóa chất chạy PCR gồm: Đệm, MgCl 2 , dNTPs, enzym kéo dài chuỗi, nước khử ion vô trùng. - Các mồi đặc hiệu gồm 12 mồi để xác định đồng thời 5 đột biến mất đoạn; đồng thời cho biết tính đồng hợp, dị hợp tử đột biến và 1 cặp mồi sử dụng làm chứng nội kiểm (Internal control). Theo bảng sau: Mồi Trình tự SEA CF 5’ - CTC TGT GTT CTC CAG TAT TGG AGG GAA GGA G - 3’ SEA NR 5’ - TGA AGA GCC TGC AGG ACC AGG TCA GTG ACC G - 3’ SEA MR 5’ - ATA TAT GGG TCT GGA AGT GTA TCC CTC CCA - 3’ FIL MF 5’ - AAG AGA ATA AAC CAC CCA ATT TTT AAA TGG GCA - 3’ FIL MR 5’ - GAG ATA ATA ACC TTT ATC TGC CAC ATG TAG CAA - 3’ THAI MF 5’ - CAC GAG TAA AAC ATC AAG TAC ACT CCA GCC - 3’ THAI MR 5’ - TGG ATC TGC ACC TCT TGG GTA GGT TCT CTA CC - 3’ α2/3.7 F 5’ - CCC CTC GCC AAG TCC ACC C - 3’ 3.7/20.5R 5’ - AAA GCA CTC TGA GGG TCC AGC G - 3’ α2 R 5’ - AGA CCA GGA AGG GCC GGT G - 3’ 4.2 R 5’ - CCC GTT GGA TCT TCT CAT TTC CC - 3’ 4.2 F 5’ - GGT TTA CCC ATG TGG TGC CTC - 3’ - Thạch agarose, đệm tra mẫu, thang chuẩn ADN. - Thuốc nhuộm ethidium bromide. 3. Bệnh phẩm 2 ml máu ngoại vi đựng trong ống chống đông EDTA. 4. Phiếu xét nghiệm Có đầy đủ các thông tin cần thiết về người bệnh, về chẩn đoán và yêu cầu xét nghiệm. V. CÁC BƯỚC TIẾN HÀNH 1. Lấy bệnh phẩm 2 ml máu ngoại vi chống đông bằng EDTA. 2. Tiến hành kỹ thuật 2.1. Tách chiết ADN Xem bài “ Tách chiết ADN từ máu ngoại vi” 175 2.2. Thực hiện phản ứng Multiplex PCR phát hiện 5 đột biến bệnh alpha thalassaemia. STT Sinh phẩm Thành phần (µl)/1 pứ 1 H 2 O 5,5 2 2X GoTaq Green MM 12,5 3 DMSO 1,0 4 Hỗn hợp mồi 5,0 5 DNA 1,0 Tổng thể tích 25,0 - Ly tâm nhẹ để các thành phần lắng xuống đ��y ống hoàn toàn - Đặt vào máy PCR - Chọn chương trình - Bấm start để máy chạy 2.3 Điện di sản phẩm PCR 2.3.1. Chuẩn bị gel agarose - Cân 1 g agarose cho vào bình thủy tinh chịu nhiệt. - Thêm 100 ml đệm điện di (TAE 1X /TBE 1X...) vào bình, lắc đều. - Đun mỗi lần 1 ph.Bài viếtPCR CHẨN ĐOÁN BỆNH ALPHA THALASSEMIA (05 ĐỘT BIẾN) xuất hiện lần đầu tại website http://khamgiodau.com

[infoglitch]

Alright then. It seems I have to pause the glitch cycle.

Let's fucking go.

SHOW ME WHAT YA GOT SPAHHZY!

..I think it's safe to say I won the angst war between me, @spahhzy, and @epic-arc. With three solid angst posts and both of them being radio silent for a minute (I feel like I should be worried about that but eh). I feel like I just hit a fuckin black flash..

is that cringe?

Ah who gives a shit! I won!

Now it's time for the glitch cycle! Let the suffering begin!

But before that, back to your regular scheduled program of lovey dovey Bullshit and me being fuckin GOOFY.

[bucknastysbabe]

i need to see fabien and tgc together soon or i shall evaporate

ANYONE SEE THOSE TWO MFS TOGETHER?????