#Sterile Environment
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Biological Safety Cabinets: An Essential Piece of Equipment for Microbiological Laboratories

Biological Safety Cabinets Microbiological safety cabinet, also known as biosafety cabinets, are essential equipment used in microbiological laboratories to protect researchers, laboratory personnel, and the environment from exposure to infectious agents. There are three main classes of microbiological safety cabinet - Class I, Class II, and Class III - with Class II being the most commonly used type in academic and pharmaceutical laboratories. Each class has different features and protection levels depending on the types of procedures being conducted. Class I Biological Safety Cabinets Class I microbiological safety cabinet, also called biosafety cabinets class I, provide personnel and environmental protection but no product protection. Airflow in Class I cabinets is drawn through the work area and directed out through an exhaust plenum. This design provides protection from microorganisms suspended in the air inside the cabinet. However, the cabinet offers no barrier between the product and sterile internal environment. As such, Class I cabinets are typically used for work that does not require product protection, such as for storage of non-hazardous material or preparation of low to moderate risk biological agents. Class II Microbiological safety cabinet Class II microbiological Biological Safety Cabinets come in Type A1, A2, B1, and B2 configurations with varying degrees of personnel, environmental, and product protection. All Class II cabinets provide heated, HEPA-filtered air to the work zone that separates the work from the external environment. The downward laminar flow provides product and environmental protection by preventing contamination of the work surface and material inside the cabinet. Class II Type A1 and A2 cabinets draw 70% of the filtered air across the work area and 30% across the HEPA filters at the rear grill. These types are suitable for low to moderate risk biohazardous work. Class II Type B1 and B2 cabinets re-circulate over 70% of the air through additional HEPA filtration for enhanced containment and are used for more hazardous manipulations. Class III Microbiological safety cabinet Class III microbiological safety cabinet, also known as glove boxes or laminar flow biosafety cabinets class III, provide the highest level of containment. They are completely enclosed with closed front, HEPA-filtered inlet, and HEPA-filtered exhaust air that passes through two sets of HEPA filters or highefficiency particulate absorption (HEPA) filters. The user manipulates materials inside the cabinet through attached rubber gloves that are permanently sealed to the access ports or openings in the cabinet. Class III cabinets are specifically designed for work with agents requiring Biosafety Level 4 practices and containment, such as highly pathogenic microbes that pose a high risk of life-threatening disease. Certification of Microbiological safety cabinet Ensuring proper installation and operation of microbiological safety cabinet is critical for personnel and environmental protection. As such, all microbiological safety cabinet must undergo certification upon installation to verify correct airflow patterns and HEPA filter integrity. Routine recertification is typically required annually to certify the cabinet is functioning as intended. The certification process involves smoke studies, inflow velocity measurements, and HEPA filter leak tests to National Sanitation Foundation (NSF) standards. Properly certified and maintained microbiological safety cabinet provide the highest level of protection for laboratory personnel working with biohazardous materials. Get more insights on Biological Safety Cabinets
Vaagisha brings over three years of expertise as a content editor in the market research domain. Originally a creative writer, she discovered her passion for editing, combining her flair for writing with a meticulous eye for detail. Her ability to craft and refine compelling content makes her an invaluable asset in delivering polished and engaging write-ups.
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#Biological Safety Cabinets#Containment#Laboratory Safety#Biosafety#Hepa Filter#Clean Air#Sterile Environment#Hazardous Materials#Ventilation System
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Reflection
Everything was white. The snow outside, her gown, her sheets, the walls, the railings on her bed, the machines beeping around her. It felt like a hospital room. It was certainly as cold as one. She found the small plastic rectangle by her pillow, pressing the button as firmly as she could.
Her father came in, and she beamed, reaching out and wheezing her request. The blankets he brought were white, and so was the tray her breakfast was on. She pressed the button to raise her bed herself, and managed to eat everything but the toast.
Something shimmered in the corner of her eye, but she ignored it as her father gently disentangled her from the machinery. He walked her to the bathroom, and as soon as the door closed, the shimmering reappeared, enveloping her.
She panicked, but was no longer in control of herself.
"I won't be long," something promised, speaking with her voice, with her mouth. "I'm just here to see you get what you deserve."
The panic multiplied, though the thing tried to soothe. What did she deserve?
It smiled at her through her reflection. "You deserve to see someone in the mirror who loves you."
#cassi writes#self loathing#sterile environment#chronic illness#severe illness#loss of bodily control
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John Gaius is really the stupidest motherfucker on the planet cause he was working in cryogenics to save the Earth and the Earth gives him magic to Make People Survive Long Term Cryogenics and then he spends months faffing around with his two pet cadavers and live-streaming and building cow walls and wondering “what am I meant to do with these awesome powers” gee if only there had been some context clues, JOHN.
#the locked tomb#tlt Alecto#john gaius#tlt spoilers#like fuck I can’t believe the whole time that the necromancy was a side effect#YOU WERE NEVER MEANT TO RESURRECT ANYONE JOHN#JUST PUT THEM ON ICE FOR A FEW THOUSAND YEARS#HELL YOU DON’T EVEN NEED THE SPACESHIPS FOR THAT#the trillionaires want to fuck off to Sirius? Let em! They can live miserable lives in sterile space environments#you’ll be healing your beautiful functioning planet while most of humanity sleeps away#hell you could probably terraform mars as a side project
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When it rains, it pours / Futtara Doshaburi Episode 5 - When it rains, it pours
#Futtara Doshaburi#abg float#When it rains it pours#When it rains it pours abg#wirip05#wirip05a#getting wet vs a sterile environment
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Overhaul WIP 🖤
#kai chisaki#overhaul#bnha#my hero academia#bnha fanart#mha fanart#overhaul fanart#my art#traditional art#art wip#he’s in a sterile environment I promise
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an argument could be made that today (February 23rd) is Bell’s birthday, In fact i am making that Argument, Everyone wish Bell a happy birthday they deserve one too 🎉🎉
#Bell as we know them of course.#And allow me to fight demons here and explain why today would be technically their birthday rather than some random day of the five weeks-#They were tortured.#The lab may have been where they were created- but not where they were born. Id say them being let out into the wild and out of the sterile#And controlled environment specifically purposed for their creation counts better. Hence today when they were brought to Berlin is-#Their birthday. After all they also definitely weren’t fully settled in previously considering how everyone was#Towards them at first. (Looking at Adler mostly here)#anyways thanks for coming to my Ted talk- happy birthday bell!!!.. guys tell them happy birthday too-everyone sing!!!#bocw#bell cod#cod bell
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Phew! Just disinfected the nursery with Virkon, literally scrubbed every wall, the floor, the doors, the window, as well as the brooder and brooder plate, the big crate (that I’ll use once the chicks are a few weeks old), even the trash can! I’m a bit paranoid about the babies getting sick, but that’s because they’re my babies. You know how strict obstetrical wards/NICUs are, because you don’t want to take risks with your babies when they’re just born and have such weak immune systems. I have the same philosophy. So I always change clothes when I have to go between the coop and the nursery, I wear a shower cap in the coop so I don’t get dander in my hair, and of course I wash my hands throughly. If it’s within my power, I’ll do it to keep the babs safe. I’ll even vaccinate them myself if I have to. I hope it doesn’t come to that, it’s expensive and I’ve never given an injection before, but I’ll do it.
#in full mother-hen mode now#the Marek’s vaccine is subdural (I think that’s the word? under the skin basically) so it’s not hard to do#and if you keep them in a sterile environment the vaccine doesn’t have to be given on day 1#it’s effective as long as the chick hasn’t been exposed to the virus yet#which is another reason I’m going so crazy with sterilizing everything
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I'm your biggest fan and willing to fight people about it
i think i know of at least one person who would be willing to fight you over that~🎃
#fan is so wild to me???#ily???#irl bestie is an absolute marshmallow though#she’s never over here on this side of tumblr tho so i’m pretty certain i won’t get shit from her for calling her that#but she is#i love her and she is an angel baby#like she almost had an aneurism when we were talking about what we kept in our first aid kits#let’s just say she’s fucking prepared for any procedure in a sterile environment#frnkiebby#fruityfroggyfelon#frank iero#mcr#frnkiero#mcrmy#mcr5#frnkie#my chemical romance#my chem#ilhsm
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rewatching Madoka to prep for the new movie. this shit still goes hard. not sure I would love it as much as I do if I hadn’t watched it as a teenager but boy howdy do I find the emotions swelling in my chest again
#I forget just how like. Oppressive the atmosphere is#every environment is sterile and uncomfortable and isolated#The witch’s labyrinths are the only place that don’t feel horribly cold and empty and it’s because they’re raw unfettered trauma#and I find something really potent in that as an aesthetic choice#Madoka Magica
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omg dude the bit in the last chapter where you mentioned the vought scientists waking homelander up randomly and shoving pictures of faces in his face to time how quickly he'd react with a smile. oooooooh boy. why does that feel so much like something they'd have done to him??????? argh going feral here!
I HONESTLY THINK ABOUT HIS LAB UPBGRINING AN UNHEALTHY AMOUNT because like. all they ever show is the Tortures but there is this one extra bonus clip that lives in my brain rent-free and i think is a much better showcase of what his lab childhood looked like, i thik i genuinely like this little clip more than i like the entirety of the lab ep from s4 FGFIGHIFGH
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CAUSE LIKE. IT COULDNT HAVE BEEN JUST. POINTLESS TORTURES IN THE LAB because otherwise they'd just have an unsocialized feral child instead of a future Vought spokesperson. in my head, homelander's lab upbringing involved a lot of invasive tortures "in the name of science" but it had to involve a LOT of social training and indoctrination as well--especially towards the end i think!
like, once he started to get older, they had to prepare him for the real world, start teaching him lines, how to act, how to play for cameras, how to interact with other heroes, etc, etc. he's an industry plant to the nth degree and to me that probably came with a lot of insane lessons. i assume that as he got older, the Tortures lessened and instead they really cranked up the lessons to make sure he's THEEEE best vought product ever, acts the right way, smiles the right way, says the right things. it didn't work straight away cuz he mentioned he just fauckin flew away during his first rollout before the stakeholders, but in the end, he did end up being the best vought promoter in the history of company (i assume, its hard to tell how big soldier boy was)
ALL OF THIS TO SAY, there was so much more that went on in those labs, it wasn't just gratuitous torture IT WENT DEEP. and i wish the show had more of that.
#i love the like. SCP-ness of little homelander in the labs. its so good. he was probably sooo creepy#i tried to show how like. unnatural he wouldve been after years and years of being socialized in such a strange sterile environment#and then the longer hes on the surface the more human he becomes bc he starts interacting with other people and also the Trauma catches up
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𖤐 Mild Gore ( simplified exposed muscle)
𖤐 Blood
𖤐 Cannibalism
Butcher Vanity
We discovered canine teeth have evolved in special ways to help each species kill and eat their favourite prey – helping to make mammals some of nature's most successful predators
Dividers 1 and 2
#butcher vanity#meat#fleshcore#tw blood#cannibalistic#implied cannibalism#aughauagahagahahahagahagah#can you tell what song I really like right now#the boys#the boys homelander#homelander#cw: gore#cw blood#kinda leaning into the horror aspect of The Boys#not the lingering background horror you guys do a great job with that already#I mean the inhuman horror of like#the cruel experiments needed to make someone that fucked up#cause damn in hindsight maybe abusing and isolating a child in such a cold and sterile environment wasn't a good idea
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this is so crazy like dont yall have grandmothers you have to talk to or hell just other human beings that exist in your real life….if u were this nitpicky irl you would end up interacting with maybe 1 person.. but maybe that’s what u do
#it’s difficult to learn after growing up in online spaces but the immense level of curation on tumblr for example#the enforcedly sterile social environment#is not sustainable in peer-to-peer interaction. everyone is problematic. and some of it does not matter at all
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ofc im scared of the dentist they share a parking lot with taco bell. They're gonna rinse my mouth with baja blast. The pick they use in my mouth is probably used to clean the crunchwrap supreme machine. The squishy fluoride foam treatment is probably just liquid cheese :(
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code black's medical accuracy goes to shit every episode but by god do they give these characters layers
#code black#first episode with ethan and ho boy#am I read to have that unpacked#also whats up with them operating in non sterile environments like every 3 episodes#why are you so crazy about that#ace is ranting
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its crazy how much my bodily self confidence etc will evaporate in a second in adverse circumstances like oh im sitting faggy. I look weak. my postures really bad. my arms look like noodles. my voice is thin and delicate. Etcetc
#I hadnt felt like this in a very long time maybe a couple years#but im in a sterile quiet medical environment where i dont speak the language and everyone knows im trans and im like#supposed to be a guy but i look like this and . Idk
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Me: Damn it, Charlie. Every time you post on Instagram you make me want to re-pierce my tongue and get an eyebrow piercing. I've never in my life wanted an eyebrow piercing! Charlie: How long did you have your tongue pierced? Me: Erm... 10, 15 years? Charlie: How long has it been out? Me: 2 to 5 years? Charlie: Stick out your tongue. Me: *immediately complies* Charlie: Yeah that's still open - I can taper that right now if you want! Me: ... This was unexpected but SURE why not! Me, two hours later: Oh yeah, the healing period is the why not. Guess it's soup and noodles for me...
#Chattering in Black and White#Honestly Charlie could talk me into a life of crime and I'd be happy to do it... he's too powerful!#(Note: This was all done in a sterile environment with the correct instruments and done by a trained piercer)
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