#Pretreatment of Biomedical Waste
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ficisid · 2 years ago
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ISID modules contains all the listing of chapters related to Waste Management and Management of Bio- medical Waste.
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biomedres · 4 years ago
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Biomedical Open Access Journals - BJSTR Journal
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Fermentation of Pretreated Herbaceous Cellulosic Wastes to Ethanol by Anaerobic Cellulolytic and Saccharolytic Thermophilic Clostridia by Kutateladze L in Biomedical Journal of Scientific & Technical Research
https://biomedres.us/fulltexts/BJSTR.MS.ID.001945.php?ts=1612498665
Lignocellulosic waste streams from agriculture, paper and wood industry are renewable, plentiful and low-cost raw materials that can be used for large-scale production of liquid and gaseous biofuels. As opposed to prevailing multi-stage biotechnological processes developed for bioconversion of cellulosic substrates to ethanol where high cost cellulase preparations are used, consolidated Bioprocessing (CBP) offers to accomplish cellulose and xylan hydrolysis followed by fermentation of both C6 and C5 sugars to ethanol in a single-stage process. Syntrophic microbial consortium comprising of anaerobic, thermophilic, cellulolytic, and saccharolytic bacteria in the genus Clostridia with improved ethanol productivity and high tolerance to fermentation end-products had been proposed for achieving CBP. 65 new strains of anaerobic thermophilic cellulolytic and saccharolytic Clostridia were isolated from different wetlands and hot springs in Georgia.
Using new isolates, fermentation of mechanically pretreated wheat straw and corn stalks was done under oxygen free nitrogen environment in thermophilic conditions (T=550C) and pH 7.1. Process duration was 120 hours. Liquid and gaseous products of fermentation were analyzed on the daily basis using Perkin Elmer gas chromatographs with flame ionization and thermal detectors. Residual cellulose, xylan, xylose and glucose were determined using standard methods. Cellulolytic and saccharolytic bacteria strains degraded mechanically pretreated herbaceous cellulosic wastes and fermented glucose and xylose to ethanol, acetic acid and gaseous products like hydrogen and CO2. Specifically maximum yield of ethanol was reached at 96 h of fermentation and varied between 2.9 - 3.2 g/ 10 g of substrate. Content of acetic acid didn't exceed 0.35 g/l. Other volatile fatty acids were detected in trace quantities.
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Production of Valuable Nanoparticles from Waste Protein Fibres-Juniper Publishers
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Nowadays, recycling natural waste materials is the most appropriate way of dealing with the problems arising from disposal of large volumes of synthetic polymers in the environment. Among the natural materials, keratin proteins are potentially useful biopolymers for various applications from agriculture to biomedical. Keratin, which largely presents in poultry feather, wool, and hair, can be developed into various shapes and forms of foams, sponges, mats, films, gels, fibers, and powder. Since the protein powder could keep the original properties of the material without destroying the microstructure, it has been widely applied in modern industries. Nanoparticles can be produced by different methods such as milling, spray drying, electro spraying, rotary blades and sonication. The recovered keratin from natural resources in powder form has promising potential for a wide range of applications including cosmetics, filtration, composites, etc. This paper overviews a brief discussion on the subject.
Key words: Protein; Natural fibers; Keratin; Nanoparticles; Enzyme
    Introduction
Nanoscale materials have received attention owing to their high surface area to volume ratio and the unique chemical and physical properties. Recently, the research on the preparation of nanoparticles from natural polymers is given much importance [1]. Natural fibers, well known as environmental friendly materials, have the advantages of low density, low cost, and biodegradability. However, a considerable amount of these fibers has been wasted during industrial processes all over the world [2]. Protein wastes such as by-products from agricultural sources, wool textile industry, poor quality raw wool not fit for spinning, and hairs and feathers from butchery constitute an important renewable source of biopolymers [3]. Keratin is the major structural fibrous protein providing outer covering such as hair, wool, feathers, and nails with robust mechanical properties, thermal durability and resistance to physicochemical degradation. The intermolecular bonding of disulfide cystine amino acid and inter- and intra molecular bonding of polar and nonpolar amino acids in keratin results in its high stability and distinctive physical characteristics [4]. Wool is a natural fiber obtained from the fleece of sheep. This natural and multicellular staple fiber is mainly composed of proteins and organic substances [5]. Feathers are probably the most abundant protein material in nature. They represent from 5% to 7% of the body weight of chickens, being important by-products of the poultry industry, which are produced millions of tons annually throughout the world [6,7]. Recycling of these protein fibers in different forms such as films, gels, scaffolds, sponges and powders has been the objective of many studies due to their high protein content, biodegradability, and biocompatibility [8-10].
The protein powder could keep the original properties of the material without destroying the microstructure [11]. Many researchers have tried to produce particles by different methods such as regeneration from keratin solution, mechanical attrition and chemical-mechanical techniques [12-16]. Solution routes have inherent limitations in the preparation process, i.e. long time of dialysis, high production costs, safety and environmental constraints. Although mechanical attrition, which involves chopping and crushing the fibers with suitable milling machines can avoid these problems, it has high energy consumption [14]. Furthermore, this process is relatively difficult due to the softness and elasticity of the natural fibers leading to dispersity in particles shapes and size. Thus, special chemical pretreatments are usually employed to weaken the fibers structure and improve powder productivity [15]. The electro-spraying techniques can be also used to develop micro- and nanoparticles form natural polymers [16]. However, this process requires extraction of keratin solution from protein fibers at first. Several attempts have been made for the extraction of keratin using chemical, mechanical and enzymatic methods [17]. The presence of high cross-linking by disulfide bonds, hydrophobic interactions and hydrogen bonding in keratin makes it hard to extract [4].
The necessary step for keratin extraction is chemical deconstruction through targeted breaking of intermolecular disulfide bonds. This can be achieved by alkaline hydrolysis, reduction, oxidation, or sulfitolysis procedures [6]. Keratin hydrolyzed by alkaline hydrolysis was fragmented into small peptide fractions with low molecular mass having ease of solubility, while keratin from reduction hydrolysis provided higher molecular mass protein homologs with limited solubility but greater potential to form keratin products particularly for biomedical applications [18]. It should be also noted that the reductive or oxidative agents used for disulfide breakage, such as thiols or peroxides, are harmful, often toxic, and difficult to handle. On the other hand, enzymatic hydrolysis is the most attractive method from the environmental point of view, due to relatively mild treatment conditions and the preservation of functional properties of the hydrolysis products [3]. We have investigated production of nanoparticles from wool and feather fibers by enzymatic hydrolysis followed by ultrasonic treatment. The results indicated the positive effect of sonication on reducing particles size after enzymatic hydrolysis, while maintaining the basic chemistry and structural integrity of the native keratin protein [19-21].
    Conclusion
Natural fibers are widely used materials in the textile industry because of their unique excellent properties. In recent years, there has been considerable interest in finding new applications of natural fibers based on regenerated protein through development of new products such as nanoparticles. Ultrafine powder can be prepared either by solution routes or by mechanical attrition of fibers. In the solution route, keratin should be extracted first by different chemicals and then particles from the aqueous solution are regenerated through a number of different methods such as spray drying. The mechanical method, on the other hand, avoids lengthy, costly and environmentally sensitive production procedures corresponding to the solution route. However, it requires special milling machines having high energy consumption. As a result, green and economical process has been attempted by researchers. For instance, we have shown that nanoparticles could be produced from waste protein fibers by enzymatic hydrolysis followed by ultrasonication, which is believed to be the most promising approaches because of the milder process conditions leaving no harmful by product. These protein nanoparticles have applications in various fields including cosmetics, coatings, filters, composites, dressings, tissue engineering, regenerative medicine and drug delivery, thanks to properties such as biocompatibility and biodegradability.
To know more about Journal of Fashion Technology-https://juniperpublishers.com/ctftte/index.php
To know more about open access journals Publishers click on Juniper Publishers
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ficisid · 2 years ago
Text
ISID modules contains all the listing of chapters related to Waste Management and Management of Bio- medical Waste.
0 notes
ficisid · 2 years ago
Text
ISID modules contains all the listing of chapters related to Waste Management and Management of Bio- medical Waste.
0 notes
ficisid · 2 years ago
Link
ISID modules contains all the listing of chapters related to Waste Management and Management of Bio- medical Waste.
0 notes
ficisid · 2 years ago
Link
ISID modules contains all the listing of chapters related to Waste Management and Management of Bio- medical Waste.
0 notes
ficisid · 2 years ago
Link
ISID modules contains all the listing of chapters related to Waste Management and Management of Bio- medical Waste.
0 notes
ficisid · 2 years ago
Link
ISID modules contains all the listing of chapters related to Waste Management and Management of Bio- medical Waste.
0 notes
ficisid · 2 years ago
Link
ISID modules contains all the listing of chapters related to Waste Management and Management of Bio- medical Waste.
0 notes
Text
Production of Valuable Nanoparticles from Waste Protein Fibres-Juniper Publishers
Journal of Fashion Technology- Juniper publishers
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Abstract
Nowadays, recycling natural waste materials is the most appropriate way of dealing with the problems arising from disposal of large volumes of synthetic polymers in the environment. Among the natural materials, keratin proteins are potentially useful biopolymers for various applications from agriculture to biomedical. Keratin, which largely presents in poultry feather, wool, and hair, can be developed into various shapes and forms of foams, sponges, mats, films, gels, fibers, and powder. Since the protein powder could keep the original properties of the material without destroying the microstructure, it has been widely applied in modern industries. Nanoparticles can be produced by different methods such as milling, spray drying, electro spraying, rotary blades and sonication. The recovered keratin from natural resources in powder form has promising potential for a wide range of applications including cosmetics, filtration, composites, etc. This paper overviews a brief discussion on the subject.
Key words: Protein; Natural fibers; Keratin; Nanoparticles; Enzyme
    Introduction
Nanoscale materials have received attention owing to their high surface area to volume ratio and the unique chemical and physical properties. Recently, the research on the preparation of nanoparticles from natural polymers is given much importance [1]. Natural fibers, well known as environmental friendly materials, have the advantages of low density, low cost, and biodegradability. However, a considerable amount of these fibers has been wasted during industrial processes all over the world [2]. Protein wastes such as by-products from agricultural sources, wool textile industry, poor quality raw wool not fit for spinning, and hairs and feathers from butchery constitute an important renewable source of biopolymers [3]. Keratin is the major structural fibrous protein providing outer covering such as hair, wool, feathers, and nails with robust mechanical properties, thermal durability and resistance to physicochemical degradation. The intermolecular bonding of disulfide cystine amino acid and inter- and intra molecular bonding of polar and nonpolar amino acids in keratin results in its high stability and distinctive physical characteristics [4]. Wool is a natural fiber obtained from the fleece of sheep. This natural and multicellular staple fiber is mainly composed of proteins and organic substances [5]. Feathers are probably the most abundant protein material in nature. They represent from 5% to 7% of the body weight of chickens, being important by-products of the poultry industry, which are produced millions of tons annually throughout the world [6,7]. Recycling of these protein fibers in different forms such as films, gels, scaffolds, sponges and powders has been the objective of many studies due to their high protein content, biodegradability, and biocompatibility [8-10].
The protein powder could keep the original properties of the material without destroying the microstructure [11]. Many researchers have tried to produce particles by different methods such as regeneration from keratin solution, mechanical attrition and chemical-mechanical techniques [12-16]. Solution routes have inherent limitations in the preparation process, i.e. long time of dialysis, high production costs, safety and environmental constraints. Although mechanical attrition, which involves chopping and crushing the fibers with suitable milling machines can avoid these problems, it has high energy consumption [14]. Furthermore, this process is relatively difficult due to the softness and elasticity of the natural fibers leading to dispersity in particles shapes and size. Thus, special chemical pretreatments are usually employed to weaken the fibers structure and improve powder productivity [15]. The electro-spraying techniques can be also used to develop micro- and nanoparticles form natural polymers [16]. However, this process requires extraction of keratin solution from protein fibers at first. Several attempts have been made for the extraction of keratin using chemical, mechanical and enzymatic methods [17]. The presence of high cross-linking by disulfide bonds, hydrophobic interactions and hydrogen bonding in keratin makes it hard to extract [4].
The necessary step for keratin extraction is chemical deconstruction through targeted breaking of intermolecular disulfide bonds. This can be achieved by alkaline hydrolysis, reduction, oxidation, or sulfitolysis procedures [6]. Keratin hydrolyzed by alkaline hydrolysis was fragmented into small peptide fractions with low molecular mass having ease of solubility, while keratin from reduction hydrolysis provided higher molecular mass protein homologs with limited solubility but greater potential to form keratin products particularly for biomedical applications [18]. It should be also noted that the reductive or oxidative agents used for disulfide breakage, such as thiols or peroxides, are harmful, often toxic, and difficult to handle. On the other hand, enzymatic hydrolysis is the most attractive method from the environmental point of view, due to relatively mild treatment conditions and the preservation of functional properties of the hydrolysis products [3]. We have investigated production of nanoparticles from wool and feather fibers by enzymatic hydrolysis followed by ultrasonic treatment. The results indicated the positive effect of sonication on reducing particles size after enzymatic hydrolysis, while maintaining the basic chemistry and structural integrity of the native keratin protein [19-21].
    Conclusion
Natural fibers are widely used materials in the textile industry because of their unique excellent properties. In recent years, there has been considerable interest in finding new applications of natural fibers based on regenerated protein through development of new products such as nanoparticles. Ultrafine powder can be prepared either by solution routes or by mechanical attrition of fibers. In the solution route, keratin should be extracted first by different chemicals and then particles from the aqueous solution are regenerated through a number of different methods such as spray drying. The mechanical method, on the other hand, avoids lengthy, costly and environmentally sensitive production procedures corresponding to the solution route. However, it requires special milling machines having high energy consumption. As a result, green and economical process has been attempted by researchers. For instance, we have shown that nanoparticles could be produced from waste protein fibers by enzymatic hydrolysis followed by ultrasonication, which is believed to be the most promising approaches because of the milder process conditions leaving no harmful by product. These protein nanoparticles have applications in various fields including cosmetics, coatings, filters, composites, dressings, tissue engineering, regenerative medicine and drug delivery, thanks to properties such as biocompatibility and biodegradability.
To know more about Journal of Fashion technology : https://juniperpublishers.com/ctftte/index.php
To know more about open access journals Publishers click on Juniper Publishers
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