#Nucleic Acid Sample Preparation Market
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DNA/RNA Sample Preparation is a series of laboratory processes used to extract, purify, and prepare nucleic acids (DNA or RNA) from various biological samples (such as blood, tissue, or cells) for subsequent analysis or experimentation
The Global DNA/RNA Sample Preparation Market is a rapidly growing segment in the healthcare industry, driven by the increasing demand for accurate and sensitive methods to monitor and manage cancer patients.
The DNA/RNA Sample Preparation market was valued at $1.67 billion in 2023 and is expected to reach $6.67 billion by 2033, growing at a CAGR of 14.81% between 2023 and 2033.
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DNA and RNA Sample Preparation Market is Estimated to Witness High Growth Owing to Increasing Adoption
The DNA and RNA sample preparation market involves processes associated with isolation, extraction, purification and quantification of nucleic acids DNA and RNA from various sources like tissues, blood, sperm, cells etc. for downstream applications in genomics, molecular diagnostics, personalized medicine and others. The sample preparation is a critical and initial step before conducting various genomic tests including Next Generation Sequencing, polymerase chain reaction and other assays. Growing awareness and adoption of precision medicine and genetic/molecular testing is driving demand for efficient nucleic acid isolation and downstream analysis.
The Global DNA and RNA Sample Preparation Market is estimated to be valued at US$ 2262.46 Mn in 2024 and is expected to exhibit a CAGR of 5.8% over the forecast period 2024 To 2031. Key Takeaways Key players operating in the DNA and RNA sample preparation are Agilent Technologies, Inc., Becton, Dickinson and Company, Bio-Rad Laboratories Inc., DiaSorin S.p.A, F. Hoffmann-La Roche, Miroculus, Inc., Illumina, Inc., PerkinElmer, Inc., QIAGEN, Sigma Aldrich Corp., Tecan Group AG, and Thermo Fisher Scientific, Inc. Growing prominence of personalized medicine is creating opportunities for development of new sample preparation methods and kits which can extract nucleic acids from various types of samples. Rising incidence of chronic and infectious diseases worldwide is increasing diagnostic testing which will propel sample preparation market growth. Global expansion of key market players through acquisitions and partnerships with regional diagnostic labs and research institutes will further augment market revenues. Market Drivers Increasing funding for Genomic and genetic research from government bodies as well as private sector is one of the key factors driving the DNA and RNA Sample Preparation Market Size. Government initiatives aimed at large scale population screening and clinical testing for various genetic disorders, infectious diseases and cancers are also creating demand for high throughput nucleic acid preparation. Growing geriatric population and rising healthcare spending in developing nations also provides growth opportunities for market players in the forecast period.
PEST Analysis Political: Laws and regulations imposed by governments for research using DNA and RNA samples could impact the market. Changes in healthcare policies will also have effects. Economic: Factors like GDP growth, income levels, healthcare spending will drive demand. Rise in research activities and focus on precision medicine boost the market. Social: Growing awareness about personalized medicine and importance of genetic testing are important. Social trends also promote preventive healthcare and wellness. Technological: Advancements in fields like next generation sequencing, lab automation, bioinformatics are key for market growth. Miniaturization and portability of equipment expand applications. Developments in sample collection and storage methods improve efficiency. Geographical regions where the market in terms of value is concentrated include North America and Europe. North America accounts for the largest share in the global market due to presence of well-established healthcare industry and research institutes. Europe also captures notable share due to growing biotech sector and research funding. The Asia Pacific region is projected to be the fastest growing market during the forecast period. This is attributed to factors such as increasing healthcare expenditure, growing awareness, expanding biotech industry and rising government investments in research. Countries like China, India offer growth opportunities as they focus on healthcare infrastructure development.
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#Coherent Market Insights#DNA And RNA Sample Preparation Market#DNA And RNA Sample Preparation#RNA Sample Preparation#Nucleic Acid Extraction#Genetic Material Isolation#DNA Extraction#RNA Extraction#Molecular Biology#Genomic DNA
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Low-Pressure Liquid Chromatography Market : Current Insights and Demographic Trends 2024-2032
Low-pressure liquid chromatography (LPLC) is a widely used separation technique in analytical and preparative chemistry. It operates at relatively low pressures, making it ideal for purifying biomolecules, such as proteins, nucleic acids, and large peptides, which are sensitive to higher pressure techniques. LPLC is commonly used in laboratories to isolate and purify target molecules from complex mixtures. This method is particularly valued for its cost-effectiveness and its ability to handle large volumes of sample without degradation, making it indispensable in research, drug development, and bioprocessing.
The Low-Pressure Liquid Chromatography Market Size was valued at USD 7.25 billion in 2023 and is expected to reach USD 13.72 billion by 2032 and grow at a CAGR of 7.36% over the forecast period 2024-2032.
Future Scope
The future of low-pressure liquid chromatography is poised for expansion in the biopharmaceutical and biotechnology sectors. As the demand for biologics and therapeutic proteins grows, LPLC systems are expected to become more specialized to accommodate new bioprocessing needs. Enhanced column technologies, capable of faster separations and higher resolution, are expected to be developed, allowing for more efficient purification processes. Additionally, the integration of automation and AI into LPLC systems is likely to streamline operations, optimize separations, and enhance reproducibility, making these systems more efficient for large-scale purification tasks.
Trends
A significant trend in low-pressure liquid chromatography is the move toward greener, more sustainable methods. The use of environmentally friendly solvents and materials in LPLC is becoming increasingly important, driven by both regulatory pressure and corporate responsibility. Another trend is the growing demand for multi-column chromatography systems, which allow for continuous operation and more efficient purification of bioproducts. Additionally, the development of smaller, more portable LPLC systems is gaining momentum, providing laboratories with greater flexibility in their purification workflows.
Applications
Low-pressure liquid chromatography is extensively used in the purification of biological molecules, particularly in the biopharmaceutical industry. It plays a key role in isolating proteins, peptides, and nucleic acids for research and therapeutic purposes. In drug development, LPLC is employed to purify and concentrate target compounds from complex mixtures. Academic research laboratories also utilize LPLC for purifying samples in proteomics and genomics studies. Additionally, LPLC is used in the food and beverage industry for separating and analyzing additives, nutrients, and contaminants.
Key Points
LPLC is ideal for purifying biomolecules, such as proteins and nucleic acids, from complex mixtures.
It operates at lower pressures, reducing the risk of damaging sensitive molecules.
Automation and AI integration are expected to enhance LPLC's future efficiency.
Green chemistry is influencing the development of environmentally friendly LPLC systems.
LPLC is widely used in the biopharmaceutical industry, drug development, and academic research.
Conclusion
Low-pressure liquid chromatography remains a critical tool for the purification of biological molecules in research and industry. Its cost-effectiveness, ability to handle large sample volumes, and compatibility with biomolecules make it indispensable, especially in the biopharmaceutical sector. With emerging trends like green chemistry, automation, and multi-column systems, LPLC is evolving to meet the changing demands of the industry. As the biopharmaceutical market grows, LPLC will continue to be a foundational technique in the purification and separation of complex biological compounds.
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Biotechnology Reagents and Kits Market will grow at highest pace owing to increasing R&D activities in biopharmaceutical companies
Biotechnology reagents and kits are used for several diagnostic and research applications including isolation, purification, and analysis of DNA and RNA. Some key products in this market include cell and tissue culture reagents, chromatography reagents, electrophoresis reagents, immunohistochemistry reagents, nucleic acid extraction and purification reagents, PCR reagents, flow cytometry reagents, electrophoresis reagents, and separation and filtration reagents. Growing funding for biotechnology research along with rising prevalence of chronic diseases has increased the demand for biotechnology reagents and kits. Advancements in biologics and personalized medicine require reagents and kits for molecular analysis of patient samples which has fueled market growth.
The Global Biotechnology Reagents and Kits Market is estimated to be valued at US$ 718.8 Mn in 2024 and is expected to exhibit a CAGR of 23% over the forecast period 2023 to 2030.
Key Takeaways Key players operating in the Biotechnology Reagents and Kits are Medtronic Plc, Hill-Rom Holdings, Inc. (Welch Allyn), iRythm Technologies, Inc., AliveCor, Inc., Vivalnk, Inc., Cardiac Insight Inc., VitalConnect, LifeSignals, Inc., Lâ€TMoreal Group, Dexcom, Inc., GENTAG, Inc., Abbott Laboratories, Koninklijke Philips N.V. and other prominent players. These players are focusing on new product development and launches to expand their product portfolio. For instance, in 2022 Medtronic Plc launched Clara Smart Pancreas System which uses RTSM technology to automatically suspend insulin delivery. The demand for biotechnology reagents and kits is growing owing to increasing demand of personalized medicine, genomics applications and cell and gene therapy research. Pharmacogenomics, companion diagnostics and molecular biomarkers are driving the need for reagents and assays utilized in the discovery, development and commercialization of personalized medicine. Growing R&D spending on developing new biologics and rising application of big data analytics in drug development is also fueling market growth. Technological advancements are expanding application of biotechnology reagents and kits in new areas. For example, next generation sequencing techniques have enabled large-scale sequencing of genomes which requires reagents for sample preparation and library preparation steps. Rapid diagnostics technologies are allowing point-of-care application of reagents for fast detection of infectious diseases. Automated instruments with integrated reagent kits are increasing efficiency and reproducibility of experiments in core labs. Market trends: 1. Growing preference for personalized medicine/ precision medicine. Biomarker discovery and validation requires biomarker detection assays which is propelling demand for immunohistochemistry, PCR and other reagents. 2. Increasing focus on contract research and contract manufacturing. CROs and CMOs require a continuous supply of biotechnology reagents and kits for conducting research and manufacturing activities on behalf of biopharma companies. Market Opportunities: 1. Emerging markets in Asia Pacific offer high growth potential. Countries like China, India and South Korea are witnessing increasing biotech research and bulk drug production which will drive the need for reagents in coming years. 2. Cell and gene therapy is a major area of focus. Reagents are essential for quality control testing, process development and scalable manufacturing of cell and gene therapies. This represents a lucrative opportunity for leading reagents players.
#Biotechnology Reagents and Kits Market Trend#Biotechnology Reagents and Kits Market Growth#Biotechnology Reagents and Kits Market Size
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Strategic Insights on Life Science Tools Market Size
The Life Science Tools Market size was estimated at USD 160.93 billion in 2023 and is expected to reach USD 370.34 billion by 2031 with a growing CAGR of 10.98% during the forecast period of 2024-2031.The Life Science Tools market is experiencing dynamic growth, driven by advancements in technology and an increasing focus on research and development in fields like genomics, proteomics, and cell biology. This market encompasses a wide range of products, including instruments, reagents, consumables, and software used in life sciences research. Innovations in high-throughput sequencing, CRISPR gene editing, and single-cell analysis are significantly expanding the capabilities of researchers, enabling more precise and efficient experiments.
The Life Science Tools industry report talks about trends, market segmentation, and market analysis. The report also includes an overview and an overview section. Additionally, the entire market from numerous diverse geographic areas with large economies is included in this sector. A variety of topics for market participants are highlighted and profiled in the research report, including company and financial overviews, corporate strategic plans, product portfolios, and recent developments.
The report conducts a qualitative analysis of the factors driving and inhibiting market expansion as well as prospective opportunities that could progress the sector. Consider sales, test outcomes, technology, products, and distribution methods. This thorough examination of each one includes thorough national and regional analyses. A special combination of quantitative data and qualitative insights is provided in the Life Science Tools Market report, which can help organizations realize long-term success.
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Market Segmentation
By Technology
Genomic Technology
Proteomics Technology
Cell Biology Technology
Other Analytical & Sample Preparation Technology
Lab Supplies & Technologies
By Type
Cell Culture Systems & 3D Cell Culture
Instruments
Consumables
Cell & Tissue Culture Services
Liquid Chromatography
Instruments
Consumables
Services
Mass Spectrometry
Instruments
Consumables
Services
Flow Cytometry
Instruments
Consumables
Services
Cloning & Genome Engineering
Kits, Reagents, and Consumables
Services
Microscopy & Electron Microscopy
Instruments
Consumables
Services
Next Generation Sequencing
Instruments
Consumables
Services
PCR & qPCR
Instruments
Consumables
Services
Nucleic Acid Preparation
Instruments
Consumables
Services
Nucleic Acid Microarray
Instruments
Consumables
Services
Sanger Sequencing
Instruments
Consumables
Services
Transfection Devices & Gene Delivery Technologies
Equipment
Reagents
NMR
Instruments
Consumables
Services
Other Separation Technologies
Instruments
Consumables
Services
Other Products & Services
Antibodies
General Supplies
Others
Instruments
Consumables
Services
By End User
Government & Academic
Biopharmaceutical Company
Healthcare
Industrial Applications
Others
COVID-19 Impact Analysis
Direct consequences on supply and demand, market and supply chain disruptions, effects on enterprises and financial markets, and other implications are just a few of the significant COVID-19 economic effects on the world. In the wake of the COVID-19 challenge, our foreign analysts are certain that the market will offer producers appealing options. An in-depth explanation of the circumstances, the health of the economy, and COVID-19's impact on the sector as a whole is provided by the Life Science Tools Market study.
Competitive Scenario
The competition analysis part of this study provides comprehensive profiles of the successful companies operating in the target market. The most recent developments are covered by the Life Science Tools Market analysis. It concentrates on the most popular markets, geographic regions, and commercial uses. It offers both qualitative and quantitative information on the trends, limitations, and growth prospects that will affect the market in the future.
Objectives of the Life Science Tools Market Report
Ongoing research into global market trends in manufacturing and consumption.
One of the most important components of investing is understanding market opportunities and obstacles throughout the anticipated period.
The current supply analysis overview discusses distributors, technology suppliers, and raw material suppliers' tendencies.
Key Questions Answered in the Life Science Tools Market Report
What possible market expansion prospects might there be in the significant nations and regions?
Which developing markets have the most prospects for technology advancement and uptake?
What strategies are successful market players using to seize the initiative straight away?
What are the main development tactics that large companies use to compete in the market?
How will the industry alter as a result of medium- to long-term product breakthroughs from various companies?
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Surface Disinfectant Market - Forecast(2024 - 2030)
Surface Disinfectant Market Overview
Surface Disinfectant Market size is $893m in 2019, growing at a CAGR of 8.7% during the forecast period 2020-2025. Surface Disinfectant are chemical substances that are applied to objects and surfaces for the purpose of sanitizing and killing microbes. It is used to disinfect food preparation surfaces, such as counter tops and cutting boards, owing to raw foods (meats, fish, and poultry) that contains pathogens like E. coli, Salmonella and Listeria. Hydrogen Peroxide, Quaternary Ammonium, Hypochlorite and others are the type of surface disinfectants used to disinfect frequently touched surfaces in households, hospital acquired infections and others to reduce the transmission of infectious illnesses, such as COVID-19, colds, flu and others.
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Key Takeaways
The increase of the prevention safety among the individuals in the medical and healthcare industry is set to propel the growth of the market.
Increasing consumer awareness about hygiene coupled with government initiatives promotes the Surface Disinfectant market.
Asia Pacific region is estimated to record the fastest growth rate during the forecast period 2020-2025.
By Product Type - Segment Analysis
In 2019, Hypochlorite segment dominated the Surface Disinfectant Market in terms of revenue is estimated to grow at a CAGR of 6.8%. It is the most common used type as it is available in solid (calcium hypochlorite) or liquid (sodium hypochlorite) forms. It is a bleaching product that is used in hospitals or private households all around the world to kill the germs or microorganisms which could spread the deadly virus like COVID-19 and others. It is used in isolation wards and for sanitization purposes. As the number of sanitization processes are emerging around the world owing to the recent COVID-19 outbreak, the market is estimated to grow at an exponential rate.
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By Distribution Channel - Segment Analysis
In 2019, Online Retailing segment dominated the Surface Disinfectant Market in terms of revenue. It is owing to the enhancements and availability of varieties of surface disinfectant product in all types. Online retailing provides discount which attracts lot of individuals. People find it more useful as it makes purchase easy by giving comparison on the basis of its varieties, helps in saving time. Convenience and ease of availability of products aids in the growth of the market.
Geography - Segment Analysis
In 2019, the North America region dominated Surface Disinfectant Market in terms of revenue with a market share of 42%. Growth in the market is attributed to the efficient healthcare structure and the rising cases of COVID-19 which has led to an increase in the number of surface disinfectants. As per claims of World Health Organization (WHO) the estimated statistics related to COVID-19 outbreak is 0.978 million, which would result in the growth of the market. Asia Pacific region is estimated to record the fasted growth rate during the forecast period 2020-2025.
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Surface Disinfectant Market Drivers
Increase In Personal Hygiene
The growing demand for high quality surface disinfectants across the globe is owing to the increasing need to improve hygiene in hospitals, clinics, and��ambulatory surgical centers to prevent the spread of infections (COVID-19) among patients and staff. Also the market is driven by the growing number of laboratories and the rising awareness among people about personal hygiene. The rising disposable incomes of people are the factors for the growth of the surface disinfectant market.
Surface Disinfectant Market Challenges
Heavy Adoption Of UV Radiation
The heavy adoption of UV radiation as a substitute for surface disinfectants is a major challenge for the market. Ultraviolet germicidal irradiation technique kills or restricts the effects of microorganisms by attacking their DNA and finishing the nucleic acids. The increased adoption of pre sterilization and practice of disinfecting medical instruments also act as major challenges for the surface disinfectant market.
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Surface Disinfectant Industry Outlook
Product Launches was the key strategy of the players in the Surface Disinfectant Industry. Surface Disinfectant top 10 companies include 3M Company, CarroLLClean, Johnson & Johnson, The Clorox Company, The Procter & Gamble Company, Sealed Air Corporation, Whiteley Corporation, Metrex Research, LLC, STERIS Corporation and Cantel Medical Corporation.
Acquisitions/Product Launches
In October 2019, 3M Company acquired Acelity, Inc. This acquisition helped the company in enhancing its product portfolio in the field of woundcare and market worldwide.
In September 2019, CarroLLClean acquired Tomco-Harwel Industries Inc, to build a strong legacy of quality and customer service while creating more opportunities for customers.
#Surface Disinfectant Market#Surface Disinfectant Market Share#Surface Disinfectant Market Size#Surface Disinfectant Market Forecast#Surface Disinfectant Market Report#Surface Disinfectant Market Growth
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Global Nucleic Acid Medicine Market Dynamics and Strategies for Success 2024-2034
The Nucleic Acid Medicine market report offered by Reports Intellect is meant to serve as a helpful means to evaluate the market together with an exhaustive scrutiny and crystal-clear statistics linked to this market. The report consists of the drivers and restraints of the Nucleic Acid Medicine Market accompanied by their impact on the demand over the forecast period. Additionally, the report includes the study of prospects available in the market on a global level.
With tables and figures helping evaluate the Global Nucleic Acid Medicine market, this research offers key statistics on the state of the industry and is a beneficial source of guidance and direction for companies and entities interested in the market. This report comes along with an additional Excel data-sheet suite taking quantitative data from all numeric forecasts offered in the study.
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Key players offered in the market: Novartis Ionis Nippon Shinyaku Alnylam Sarepta Therapeutics MiNA Therapeutics BioNTech Moderna
Additionally, it takes account of the prominent players of the Nucleic Acid Medicine market with insights including market share, product specifications, key strategies, contact details, and company profiles. Similarly, the report involves the market computed CAGR of the market created on previous records regarding the market and existing market trends accompanied by future developments. It also divulges the future impact of enforcing regulations and policies on the expansion of the Nucleic Acid Medicine Market.
Scope and Segmentation of the Nucleic Acid Medicine Market
The estimates for all segments including type and application/end-user have been provided on a regional basis for the forecast period from 2024 to 2034. We have applied a mix of bottom-up and top-down methods for market estimation, analyzing the crucial regional markets, dynamics, and trends for numerous applications. Moreover, the fastest & slowest growing market segments are pointed out in the study to give out significant insights into each core element of the market.
Nucleic Acid Medicine Market Type Coverage: - ASO siRNA Other
Nucleic Acid Medicine Market Application Coverage: - Cardiovascular Diseases Hepatitis B Hypertension Other
Regional Analysis:
North America Country (United States, Canada) South America Asia Country (China, Japan, India, Korea) Europe Country (Germany, UK, France, Italy) Other Countries (Middle East, Africa, GCC)
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The comprehensive report provides:
Complete assessment of all opportunities and threats in the global market.
Nucleic Acid Medicine Market recent advancements and major events.
A thorough study of business policies for the growth of the Nucleic Acid Medicine Market leading players.
Concluding study about the growth plot of Nucleic Acid Medicine Market for upcoming years.
Detailed understanding of Nucleic Acid Medicine Market particular drivers, restraints, and major micro markets.
Favorable impression inside vital technological and market latest trends hitting the Nucleic Acid Medicine Market.
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Plan for new product promotion and portfolio in advance
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#Nucleic Acid Medicine Market#Nucleic Acid Medicine Market trends#Nucleic Acid Medicine Market future#Nucleic Acid Medicine Market size#Nucleic Acid Medicine Market growth#Nucleic Acid Medicine Market forecast#Nucleic Acid Medicine Market analysis
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Isothermal Nucleic Acid Amplification Technology (INAAT) Market size at USD 4.21 billion in 2022. During the forecast period between 2023 and 2029, BlueWeave expects Global Isothermal Nucleic Acid Amplification Technology Market size to grow at a significant CAGR of 12.29% reaching a value of USD 8.44 billion by 2029. Major growth drivers for the Global Isothermal Nucleic Acid Amplification Technology (INAAT) Marketinclude increasing prevalence of infectious diseases. Diseases such as tuberculosis, hepatitis, and influenza are major contributors to fatalities from infectious diseases, particularly in developing nations. Consequently, there is expected growth in the acceptance of INAAT solutions due to the need for swift, easy-to-use, and disease-specific testing alternatives. Ongoing advancements in existing amplification technologies, coupled with the introduction of innovative methods like Single Primer Isothermal Amplification (SPIA), Loop-Mediated Isothermal Amplification (LAMP), Recombinase Polymerase Amplification (RPA), and Strand Displacement Amplification (SDA), are creating new possibilities for INAAT product applications. These developments are expected to enhance the acceptance of the INAAT during the forecast period. Also, prospective strategic collaborations among manufacturers of INAAT diagnostics and related products are poised to play a pivotal role in augmenting market prospects and fostering industry growth. However, complex sample preparation and market competition are anticipated to restrain the overall market growth during the period in analysis.
Opportunity – Innovative applications of INAAT in emerging markets
Emerging applications for Isothermal Nucleic Acid Amplification Technology (INAAT) products result from the rapid evolution of existing amplification technologies and the introduction of innovative methods like Single Primer Isothermal Amplification (SPIA), Loop-Mediated Isothermal Amplification (LAMP), Recombinase Polymerase Amplification (RPA), and Strand Displacement Amplification (SDA). A noteworthy example is a study, published in the Analyst Journal in June 2022, utilizing the LAMP technology to demonstrate swift Zika virus testing with a smartphone-integrated device, requiring only a single blood drop. These advancements are expected to deepen the penetration of the INAAT during the forecast period.
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DNA RNA Extraction Kit Market Size, Type, segmentation, growth and forecast 2023-2030
DNA RNA Extraction Kit Market
The DNA RNA Extraction Kit Market is expected to grow from USD 1.30 Billion in 2022 to USD 2.20 Billion by 2030, at a CAGR of 7.26% during the forecast period.
DNA RNA Extraction Kit Market Size
The DNA RNA Extraction Kit market research report analyzes the global market segment based on product type (DNA Extraction Kit, RNA Extraction Kit), end-use application (Hospital, Laboratory, Other), and geography (North America, Asia Pacific, Middle East, Africa, Australia and Europe), with the leading market players including Qiagen, Thermo Fisher Scientific, Merck KGaA, Roche, Cytiva, Agilent, Danaher, Promega, Bio-Rad, Bioneer, and Akonni Biosystems. The report dives deep into the dynamics of the market and the regulatory and legal factors, such as the increasing incidence of genetic disorders and the growing demand for personalized medicine and research on gene-based therapies. The market growth can be attributed to technological advancements, increasing research and development activities, and the need for quick, accurate, and efficient extraction of nucleic acids. The report anticipates continued market growth in the future.
DNA RNA Extraction Kit Market Key Player
Qiagen
Thermo Fisher Scientific
Merck KGaA
Roche
Cytiva
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DNA RNA Extraction Kit Market Segment Analysis
The DNA RNA Extraction Kit market targets customers in research and biotechnology laboratories, pharmaceutical companies, academic institutions, and diagnostic centers. The increasing demand for effective and efficient research tools for genetic analysis and clinical diagnostics is driving the revenue growth of the DNA RNA Extraction Kit market. The rising incidence of genetic diseases, cancer, and infectious diseases have led to increased demand for DNA RNA Extraction Kits as a primary step in genetic analysis and diagnosis.
One of the latest trends in the DNA RNA Extraction Kit market is the development of fully automated and high-throughput extraction systems, which enable faster and more efficient extraction of DNA and RNA from different sample types. Moreover, the growing popularity of personalized medicine and genomic-based research is expected to fuel the demand for DNA RNA Extraction Kits in the coming years.
The major challenge faced by the DNA RNA Extraction Kit market is the high cost of these kits, which may limit their accessibility, particularly in developing countries. Additionally, quality control issues in sample preparation and the lack of standardization in DNA RNA extraction protocols may also restrain the growth of the market.
The main findings of the DNA RNA Extraction Kit market report suggest that North America holds the largest share due to the presence of major market players in the region, while the Asia-Pacific region is expected to witness the fastest growth due to the rising demand for molecular diagnostics in the region.
The report recommends market players to focus on product innovation, developing cost-effective and easy-to-use DNA RNA Extraction Kits, and expanding their global reach through strategic partnerships and collaborations. Additionally, the report suggests that regulatory bodies should establish standardized protocols for DNA RNA extraction to ensure consistency and reliability in research and clinical diagnostics.
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Market Segmentation (by Application):
Hospital
Laboratory
Other
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Liquid Handling Systems Market Worth $6.99 Billion by 2029 – Exclusive Report by Meticulous Research®
According to a new market research report, ‘Liquid Handling Systems Market By Type (Automated, Electronic, Manual), Product (Pipette, Consumables, Burette, Software, Microplate), Application (Drug Discovery, Cancer & Genomics, Clinical), End User (Hospital, Diagnostic Lab) – Global Forecast to 2029,’ published by Meticulous Research®, the liquid handling systems market is projected to reach $6.99 billion by 2029, at a CAGR of 6.4% from 2022 to 2029.
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A liquid handling system involves any equipment used for transferring liquids in a laboratory from a pipette to the multifunction workstation. Liquid handling is usually required in the laboratory for various applications such as ELISA, nucleic acid preparation, PCR reaction, next-generation sequencing for genomic research, and liquid-liquid extraction. Liquid handling systems are crucial in increasing laboratory procedures requiring high-throughput screening.
Major factors driving the liquid handling systems market are the rapid growth and expansion of the pharmaceutical & biotechnology industry, increasing R&D expenditure of biopharmaceutical companies, rapid adoption of automation in laboratories, and growing demand for high-throughput screening. Furthermore, emerging economies and growing genomics and proteomics research are expected to provide significant growth opportunities for this market.
However, the high costs of automated liquid handling systems are expected to restrain the growth of this market to a notable extent. In addition, factors such as the slow adoption of automation among small & medium-sized laboratories and additional training required by the laboratory personnel for operating automated robotic liquid handling workstations are the major challenges to the growth of this market.
The Impact of COVID-19 on the Liquid Handling Systems Market
The coronavirus disease (COVID-19) outbreak in March 2020 impacted nearly every country around the globe. According to the WHO, as of 17th November 2022, there were 633.2 million confirmed cases and 6.6 million confirmed deaths. During the initial months of the pandemic, there were nationwide lockdowns and restrictions in several countries. This led to a global supply chain disruption and shortages of equipment & consumables. Further, according to the International Monetary Fund, the pandemic shrunk the global economy by about 3.5% in 2020. These factors had a short-term negative impact on the global liquid handling systems market.
However, due to the pandemic, there was an increased demand for COVID-19 testing in laboratories. Large volumes of samples were to be tested, and liquid handling systems were a crucial part of performing these tests. This led to an increased demand for liquid handling systems in clinical diagnostics laboratories. In addition, there was an increased demand for drug discovery and vaccine development. The demand for various types of liquid handling systems in pharmaceutical and biotechnology companies also increased. Thus, the COVID-19 pandemic ultimately had a positive impact on the liquid handling systems market.
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The liquid handling systems market is segmented by Type (Automated Liquid Handling Systems, Electronic Liquid Handling Systems, and Manual Liquid Handling Systems), Product (Pipettes [Semi-Automated Pipettes, Manual Pipettes, and Electronic Pipettes], Consumables, Liquid Handling Workstations, Burettes, Software, Microplate Reagents Dispensers, Microplate Washers, and Other Products), Application (Drug Discovery, Cancer & Genomics Research, Clinical Diagnostics, and Other Applications), End User (Hospitals & Diagnostic Laboratories, Biotechnology & Pharmaceutical Industries, Research & Academic Institutes, and Other End Users), and Geography. The study also evaluates industry competitors and analyzes their market share at global and regional levels.
Based on type, in 2022, the automated liquid handling systems segment is expected to account for the largest share of the liquid handling systems market. The large market share of this segment is attributed to factors such as the increased demand for high-throughput screening, the advantages of automated liquid handling systems, and the increased capabilities of end users to acquire advanced automated liquid handling systems.
Based on product, in 2022, the pipette segment is expected to account for the largest share of the liquid handling systems market. The large market share of this segment is attributed to the basic utility of pipettes in the liquid handling process and its applicability in a wide range of applications such as in sample preparation, serial dilution, and various types of assays.
Based on application, in 2022, the drug discovery segment is expected to account for the largest share of the liquid handling systems market. This segment’s large market share is attributed to the high demand for newer personalized drugs for treating a wide range of diseases, increased R&D expenditure, and increased focus on the efficient development of new drugs through molecular screening.
Based on end user, in 2022, the biotechnology & pharmaceutical industries segment is expected to account for the largest share of the liquid handling systems market. The large market share of this segment is attributed to the higher demand for liquid handling systems in the biotechnology & pharmaceutical industry, especially for drug discovery and manufacturing applications, and increased R&D expenditure in biopharma companies.
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Based on geography, in 2022, North America is expected to account for the largest share of the liquid handling systems market, followed by Europe and Asia-Pacific. North America’s large market share is attributed to its robust healthcare and R&D infrastructure, high R&D spending and investments, collaborations with large academic universities and the presence of key players in the region.
The report also includes an extensive assessment of the type, product portfolio, application, end user, and geography, and key strategic developments adopted by leading market participants in the industry over the past years (2020–2022). In recent years, the liquid handling systems market has witnessed numerous product launches & approvals, agreements, collaborations, partnerships, and mergers & acquisitions.
Some of the key players operating in this market study are Thermo Fisher Scientific Inc. (U.S.), Agilent Technologies, Inc. (U.S.), Danaher Corporation (U.S.), Eppendorf AG (Germany), Hamilton Company (U.S.), PerkinElmer, Inc. (U.S.), Tecan Group Ltd. (Switzerland), PRIMADAG SAS (France), BRAND GMBH + CO KG (Germany), Hudson Robotics (U.S.), SPT Labtech (U.K.), F. Hoffmann-La Roche Ltd. (Switzerland), QIAGEN N.V. (Netherlands), and Mettler-Toledo International, Inc (U.S.).
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Scope of the Report:
Liquid Handling Systems Market, by Type
Automated Liquid Handling Systems
Electronic Liquid Handling Systems
Manual Liquid Handling Systems
Liquid Handling Systems Market, by Product
Pipettes
Semi-automated pipettes
Manual Pipettes
Electronic Pipettes
Consumables
Liquid Handling Workstations
Burettes
Software
Microplate Reagents Dispensers
Microplate Washers
Other Products
(Other Products include stackers, flocculation tester, pipetting aid, and laboratory glassware)
Liquid Handling Systems Market, by Application
Drug Discovery
Cancer & Genomics Research
Clinical Diagnostics
Other Applications
(Other applications include proteomics, bioprocessing in biotechnology, basic research, and microbiology)
Liquid Handling Systems Market, by End User
Hospitals & Diagnostic Laboratories
Biotechnology & Pharmaceutical Industries
Research & Academic Institutes
Other End users
(Other end users include contract research organizations, forensic laboratories, and government laboratories)
Liquid Handling Systems Market, by Geography
North America
U.S.
Canada
Europe
Germany
France
U.K.
Italy
Spain
Rest of Europe (RoE)
Asia-Pacific
China
Japan
India
Rest of APAC (RoAPAC)
Latin America
Middle East & Africa
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Trend Forecast in Molecular Biology Reagents Market
The Molecular Biology Reagents market size has grown significantly in recent years. However, due to the outbreak of the COVID-19 pandemic, the market experienced a sharp decline in 2020. Governments from all around the world are collaborating with pharmaceutical and biotech businesses to combat the COVID-19 epidemic, from fostering the creation of vaccinations to preparing for issues with the distribution of medical supplies. The Molecular Biology Reagents Market size is expected to reach USD 10.2 billion and is projected to reach a CAGR of 14.3% from 2023-2030.
Reagents are essential to the success of PCR reactions, nucleic acid separation, and restriction enzyme digestion experiments. Working with appropriate and dependable materials like buffers, water, enzymes, and chemicals is essential since molecular biology reagents may make or break your experiment or sample preparation.
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Delvens Industry Expert's Standpoint
The study of genomics and molecular biology has undergone a complete transformation thanks to the ever-expanding uses of sequencing technologies and the creation of enormous data sets. NGS's introduction made it possible to sequence DNA in a quick and inexpensive manner. Together with NGS, third-generation sequencing technology is revitalizing genome sequencing and solving long-standing issues with de novo genome assembly, haplotype phasing, and structural variation analysis. Companies are continually developing and releasing new sequencing reagents in response to the increased demand for sequencing technologies on a worldwide scale which is further anticipating the growth of the molecular biology reagent market.
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Key Findings
The Application segment is further segmented into PCR, Sequencing, Cloning, Epigenetics, Restriction Digestion, Synthetic Biology, and Other Applications. The other application segment is anticipated to dominate the market during the forecast period and sequencing held the second largest share. This development can be linked to the growing use of sequencing-based medical therapies and their application in cancer.
The End User segment is further segmented into Academic & Research Institutes, Pharmaceutical & Biotechnology Companies, Hospitals & Diagnostic Centers, and other end users. The pharma and biotech segment held the largest share. This is because molecular biology tools that streamline workflow, such as PCR, sequencing, and genotyping, are widely accepted. The pharma and biotech businesses' use of these items during the development of drug candidates, customized medicine, or diagnostics facilitates product improvements, which in turn boosts sales. The market will be driven in the next years by enterprises' extensive use of molecular technologies during R&D and clinical trials.
The market is also divided into various regions such as North America, Europe, Asia-Pacific, South America, and Middle East and Africa. The North American region is expected to be the largest region in the Molecular Biology Reagents market during the forecast period. Due to the existence of bio-clusters in countries like India and China and a rise in the outsourcing of research activities to these nations, Asia-Pacific is anticipated to have attractive development prospects in the molecular biology reagents market.
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According to regional analysis North America Dominate the Molecular Biology Reagents Market. Asia-Pacific is anticipated to have attractive development prospects in the molecular biology reagents market too.
The Key Market Players are:
Thermo Fisher Scientific, Inc., Merck KGaA, Promega Corporation, New England Biolabs, Illumina, Inc., Takara Bio Inc., Qiagen N.V., Agilent Technologies, Inc., F. Hoffmann-La, Roche Ltd., Bio Basic Inc., Jena Bioscience GmbH, Molecular Biology Resources, Inc., Bio, Rad Laboratories, Inc., Zymo Research Corporation, Integrated DNA Technologies, Inc. are the major players operating in Molecular Biology Reagents Market.
The key benefits of this report:
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The Qualitative and quantitative data utilization to discover arrays of future growth from the market trends of leaders to market visionaries and then recognize the significant areas to compete in the future.
Provides In-depth analysis of the changing trends of the market by visualizing the historic and forecast year growth patterns.
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Nucleic Acid Sample Preparation Market Future Trends to Look Out | Bis Research
Nucleic acid sample preparation refers to the set of processes and techniques employed to isolate, purify, and prepare nucleic acids (DNA or RNA) from various biological samples for downstream applications and analysis.
The global Nucleic Acid Sample Preparation Market is projected to reach $5,615.9 million by 2033 from $2,922.8 million in 2023, growing at a CAGR of 6.75% during the forecast period 2023-2033.
Nucleic Acid Sample Preparation Overview
This process is critical for ensuring the integrity and quality of nucleic acids, which are essential for reliable and accurate downstream applications such as polymerase chain reaction (PCR), sequencing, and gene expression analysis.
Key Steps in Nucleic Acid Sample Preparation
Sample Collection
Cell Lysis
Nucleic Acid Isolation
Purification
Quantification and Quality Assessment
Grab a look at the free sample page for more understanding click here !
Nucleic Acid Sample Preparation Important Considerations
Contamination - Avoiding contamination with nucleases, other nucleic acids, and environmental contaminants is critical. Use nuclease-free reagents and consumables.
Sample Handling - Gentle handling minimizes mechanical shearing of nucleic acids.
Control Samples - Always include control samples to monitor for contamination and process consistency.
Sample Collection and Preparation
Biological Source - Samples can be obtained from various sources such as blood, tissue, saliva, buccal swabs, or cultured cells.
Preservation - Immediate and appropriate preservation is crucial. For RNA, samples should be stabilized quickly using RNA stabilizing agents (e.g., RNAlater) to prevent degradation by RNases. For DNA, samples should be kept at low temperatures or preserved in ethanol.
Cell Lysis
Lysis Buffers: The choice of lysis buffer depends on the sample type and the nucleic acid to be extracted.
Mechanical Disruption: Methods like bead beating, sonication, or homogenization are often used to physically disrupt tough tissue samples.
Nucleic Acid Extraction
Phenol-Chloroform Extraction: This traditional method uses phenol and chloroform to separate proteins from nucleic acids, followed by ethanol precipitation to concentrate the nucleic acids.
Silica Column-Based Extraction: Common in commercial kits, this method involves binding nucleic acids to a silica membrane in the presence of chaotropic salts, followed by washing and elution.
Magnetic Beads: Nucleic acids bind to magnetic beads under specific conditions, allowing for easy washing and elution with the help of a magnetic field.
4. Purification
RNase and DNase Treatment: To remove contaminating RNA from DNA preparations, DNase treatment is applied, and vice versa for RNA preparations.
Column Wash Steps: Additional wash steps in column-based methods help to remove impurities such as proteins, salts, and other contaminants.
5. Quantification and Quality Assessment
Spectrophotometry: Measuring absorbance at 260 nm (A260) provides a quantitative estimate of nucleic acid concentration. The A260/A280 ratio indicates protein contamination, with ideal values around 1.8 for DNA and 2.0 for RNA.
Fluorometry: More sensitive than spectrophotometry, fluorometric assays (e.g., Qubit) use fluorescent dyes that bind specifically to nucleic acids.
Electrophoresis: Agarose gel electrophoresis can be used to assess the integrity and size distribution of the nucleic acids.
6. Storage
Short-Term Storage: Nucleic acids can be stored at 4°C for short-term use.
Long-Term Storage: For long-term preservation, DNA is stored at -20°C or -80°C, while RNA is best stored at -80°C to prevent degradation.
Nucleic Acid Sample Preparation Market Drivers
Increasing demand for molecular diagnostics
Advancements in Biotechnology and Genomics
Rising Research and Development Activities
Expansion of Biopharmaceutical Manufacturing
Key Market Players
Agilent Technologies, Inc.
Autogen, Inc.
Bio-Rad Laboratories, Inc.
Roche AG
Merck KGaA
and many others
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Market Segmentation
Product Type
End Users
Applications
Geography
Various different applications involved are as follows
Clinical Diagnostics
Biomedical Research
Pharmaceutical and Biotechnology Development
Agricultural Biotechnology
And many others
Recent Developments in the Nucleic Acid Sample Preparation Market Market
Qiagen N.V. introduced two groundbreaking additions to its sample technologies portfolio, i.e., the TissueLyser III that facilitates high-throughput disruption of diverse biological samples and the RNeasy PowerMax Soil Pro Kit that isolates high-purity RNA from challenging soil samples using advanced Inhibitor Removal Technology.
PerkinElmer introduced the CHEF Magnetic Bead Cleanup System, providing automated nucleic acid purification through advanced magnetic bead technology. This novel system would help automate the nucleic acid purification process efficiently.
Conclusion
In conclusion, the Nucleic Acid Sample Preparation market continues to experience significant growth and expansion driven by a multitude of factors. The increasing demand for molecular diagnostics, fueled by the prevalence of infectious diseases and genetic disorders, underscores the importance of nucleic acid-based testing in clinical settings.
Overall, the Nucleic Acid Sample Preparation market is poised for continued expansion, driven by ongoing advancements in technology, increasing research and development activities, and the growing demand for molecular diagnostics and personalized medicine.
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Chromatography Resins Market is driving Separation and Purification Processes
Chromatography resins help separate and purify biomolecules and are used extensively in pharmaceutical, food, and chemical separation processes. Chromatography resins are polymer beads that facilitate the separation, purification, and quantification of biomolecules like proteins, peptides, nucleic acids, and small molecules. The beads contain ligands that bind selectively to biomolecules based on their chemical or physical properties. Based on the ligand used, chromatography resins are available in different variants like ion-exchange resins, affinity resins, hydrophobic interaction resins, size-exclusion resins. When a sample mixture passes through the chromatography resin column, different biomolecules bind to the resin with varying affinity based on their characteristics and get separated. Pure components can then be collected by changing the conditions like pH or introducing an elution buffer. The Global Chromatography Resins Market is estimated to be valued at US$ 2.2 Billion in 2024 and is expected to exhibit a CAGR of 6.7% over the forecast period 2024-2027.
Rapid growth in the pharmaceutical and biotechnology industries and increasing research on drug discovery and large molecule biosimilars have boosted the demand for effective separation techniques. Chromatography resins have become integral to the purification of antibodies, vaccines, hormones, enzymes, and other therapeutic proteins. Key Takeaways Key players operating in the chromatography resins market are Thermo Fisher Scientific Inc., WR Grace & Co., Merck KGaA, Bio-Rad Laboratories Inc., Tosoh Corporation, and Purolite Corporation. The booming biopharmaceutical industry is driving consistent demand for chromatography resins for large-scale downstream processing applications. Monoclonal antibody production alone requires huge volumes of varied chromatography resins. Global players are expanding their production capacities across Asia Pacific and Latin America to meet the growing requirements of the pharmaceutical industry in emerging markets and benefit from low-cost manufacturing opportunities. Several new production plants for chromatography resins have come up over the past five years in China, India, Brazil, and Mexico. Market key trends One of the key trends gaining traction in the Chromatography Resins Market Demand is the rising popularity of pre-packed chromatography columns. These plug-and-play columns containing the resin reduce preparation time and improve reproducibility. They are well-suited for process development activities and process characterization runs involving short cycle-times. Major resin manufacturers are focusing on developing advanced pre-packed columns with better flow dynamics and higher binding capacities.
Porter’s Analysis Threat of new entrants: Low capital requirements and established value chain restrict threats of new entrants in chromatography resins market.
Bargaining power of buyers: Large number of buyers and availability of substitutes increases buyers bargaining power.
Bargaining power of suppliers: Established suppliers and intellectual property rights limit buyers bargaining power.
Threat of new substitutes: Emerging technologies pose threat of substitution to chromatography resins.
Competitive rivalry: Large number of players compete on cost and quality leading to high competitive rivalry. Geographical Regions North America accounts for the largest share in chromatography resins market in terms of value owing to increasing R&D investment and growth of pharmaceutical industry. Asia Pacific is anticipated to be the fastest growing region during the forecast period due to growing pharmaceutical industry, outsourcing trends and increasing research activities in countries like India and China.
Get more insights on Chromatography Resins Market
About Author:
Money Singh is a seasoned content writer with over four years of experience in the market research sector. Her expertise spans various industries, including food and beverages, biotechnology, chemical and materials, defense and aerospace, consumer goods, etc. (https://www.linkedin.com/in/money-singh-590844163)
#Coherent Market Insights#Chromatography Resins Market#Chromatography Resins#Purification Resins#Protein Purification#Ion Exchange Resins#Affinity Chromatography Resins#Gel Filtration Resins
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Blood Screening Market Size (2022-2030) Industry Trends, Growth, Challenges, and Forecast: Growth Plus Reports
The global blood screening market is expected to develop considerably during the forecast period. The growth can be due to various factors such as higher blood donation rates, the prevalence of chronic illnesses and infectious diseases, and expanding economies. Other market drivers include the rapid development of new technologies and the introduction of enhanced products. Another beneficial factor in the market is the increase in healthcare initiatives or investments from public and private organizations.
The blood screening market was valued at US$ 2.3 billion in 2021. The market is expected to witness a CAGR of 8.1% from 2022 to 2030.
Growing chronic illness/disease occurrences also contribute to market expansion. An increase in such incidences is a prime cause of the rise in fatalities. The risk factors include poor eating habits, physical inactivity, and use of cigarettes/tobacco and alcohol. According to the WHO (World Health Organization) report for 2021, nearly 17.9 million people die every year due to chronic diseases such as cancers (9.3 million), respiratory diseases (4.1 million), and diabetes (1.5 million). Thus, these factors encourage the growth of the market globally.
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Key players:
· Ortho-Clinical Diagnostics Inc.
· Thermo Fisher Scientific Inc.
· Abbott Laboratories
· F. Hoffmann-La Roche AG
· Grifols S.A.
· Quotient Technology Inc.
· Bio-Rad Laboratories Inc.
· Becton, Dickinson and Company
· Danaher Corporation (Beckman Coulter)
· Siemens Healthineers
By Technology:
· ELISA
· Western Blot
· RT-PCR
· Rapid Test
· Nucleic Acid Amplification
By Product:
· Software
· Instrument
· Reagent & Kits
Regional Analysis-
· The Asia-Pacific market is anticipated to grow at a higher CAGR during the forecast period.
· Major factors contributing to the growth of Blood Screening market in this region include the rising incidence of chronic infectious diseases, rising awareness regarding growing applications of Blood Screening, and increasing research activities in the field of Blood Screening.
· The emergence of local players and new product launches has also played a critical role in boosting the Blood Screening market in this region.
Key Questions Answered in the Report Include:
(1) This report gives all the information about the industry analysis, revenue, and overview, of this market.
(2) What will be the rate of increase in market size and growth rate by the end of the forecast period?
(3) What are the major global Blood Screening Market trends influencing the development of the market?
(4) What are the vital results of SWOT analysis of the major players operating in the Blood Screening Market?
(5) What are the potential growth opportunities and threats faced by Major competitors in the market?
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Why is it important to check for bacterial endotoxins in drug development?
Bacterial endotoxin is a significant source of contamination in biomedical tests and pharmaceutical products, and the inspection of bacterial endotoxin has become one of the critical testing items for drug quality control.
Impurities that need to be controlled may be generated during the production and standard storage of drugs according to established processes, and these impurities may impact the activity and safety of the drug itself. They may also cause adverse reactions in the clinical use of the drug. Therefore, impurity analysis is crucial in pharmaceutical research to drug development.
Bacterial endotoxin is a process-related impurity, one of the headache impurities in the purification of proteins, nucleic acids, polysaccharides, and other biological macromolecules, which are widely present in reagent consumables and samples. It is a significant source of contamination in biomedical tests and pharmaceutical products, and the inspection of bacterial endotoxin has become one of the critical testing items for drug quality control.
Bacterial endotoxin is a complex formed by lipopolysaccharide of the cell wall of Gram-negative bacteria, and a variety of trace proteins, which are released with biological activity when the bacteria die or disintegrate, and bacterial endotoxin is related to pyrogen. The impurities in the drug that can cause an increase in body temperature are pyrogens, mainly derived from the bacterial endotoxin of gram-negative bacteria, and the human body is susceptible to endotoxin, which can cause fever when mixed with picogram levels in each milliliter of blood.
Endotoxin is the leading cause of failure in the pyrogen testing of pharmaceuticals. Under GMP conditions, the accepted view of quality control in pharmaceutical production is that no endotoxin means no pyrogen, and control of endotoxin means control of pyrogen.
To ensure the success and reliability of biomedical tests and the safety and efficacy of biomedical products, the complete removal of bacterial endotoxins from samples and solutions is of great concern to pharmaceutical developers.
Since bacterial endotoxins can cause rapid agglutination of the blood cell lysates of a marine organism, horseshoe crab, a variety of compassionate bacterial endotoxin detection methods based on extracts of horseshoe crab blood, LAL/TAL reagents, have been derived from this. The 2010 edition of the Chinese Pharmacopoeia stipulates that the bacterial endotoxin check method is a method that uses horseshoe crab reagents to detect endotoxin or quantify bacterial endotoxin produced by gram-negative bacteria to determine whether the limit of bacterial endotoxin in the test article complies with the regulations.
Among the methods for endotoxin detection using LAL/TAL reagents, the gel method, the turbidity method, and the colorimetric method is recognized and widely used by significant biological industries.
Colorimetric and turbidimetric methods are used for impurity analysis, and the impurity-specific colorimetric (colorimetric) and precipitation reactions (turbidimetric) can be used against standards.
However, preparing LAL/TAL reagents requires capturing rare animals, horseshoe crabs, and other methods that require using LAL/TAL reagents for endotoxin detection, such as enzyme-linked immunosorbent assay and flow cytometry are coming on the market one after another. So what are the main methods used to detect bacterial endotoxin?
1, Gel method
The gel method is to use horseshoe crab reagent to quantify the detection of bacterial endotoxin produced by gram-negative bacteria, the sample to be tested is mixed with horseshoe crab reagent, and after a while, the principle of agglutination reaction between horseshoe crab reagent and endotoxin is used to determine whether the limit of bacterial endotoxin in the test article following the regulations of a method.
The method is easy and inexpensive to operate, but it is also less automated, more time-consuming, and prone to false positives. Gel chromatography and sol-gel methods are all impurity analysis methods.
For example, one researcher used the gel method to establish the bacterial endotoxin examination method for benzene sulfonylurea cisatracurium injection [1].
Methods Referring to the 2015 edition of the Chinese Pharmacopoeia (Part IV) for bacterial endotoxin examination, the inhibition of bacterial endotoxin examination by cisatracurium besylate the injection was verified by testing and dilution of the test article with diluent I instead of water for bacterial endotoxin examination (WBET) could eliminate the inhibition of endotoxin examination by Cisatracurium besilate.
The results showed that supplementing the appropriate amount of divalent ions could effectively eliminate the inhibition of bacterial endotoxin check by phenyl sulfonium cisatracurium injection. It is concluded that the maximum non-interfering concentration of 0.02 g/L can control bacterial endotoxin in benzene sulfonylurea cisatracurium injection by gel method. Medicilon provides pharmaceutical impurity analysis services, which can use techniques such as HPLC to separate impurities in regulated starting materials, intermediates, APIs, and drug products.
2, Turbidity method
The turbidimetric method to produce turbidity change by bacterial endotoxin binding to the C-factor of horseshoe crab reagent and activating coagulase to form coagulation protein. As an impurity analysis process, the turbidimetric analysis technique can also be used to help study the source of impurities in crystals and the effect of pollutants on infrared absorption and color-centered lasers.
The turbidity method is divided into the endpoint and dynamic processes according to the different measurement methods. The turbidimetric method for detecting bacterial endotoxin is simple to operate, not affected by the test environment, and has the characteristics of high sensitivity and wide detection range. However, the turbidimetric method needs to be equipped with special instruments.
For example, one researcher used the turbidimetric plan to explore the bacterial endotoxin examination of aqueous solutions of moistened burn cream after hexane extraction [2]. The method uses an additive quantitative endotoxin inoculation method to extract the burn cream endotoxin from the oil phase into the aqueous phase, and the dynamic turbidimetric method was used to determine the endotoxin recovery and to examine the effect of the extraction process on endotoxin; the gel method was also used to validate the non-interference concentration further.
Results The recovery of endotoxin after the 6-fold dilution of the extract was 52.3%. There was no interference with the reaction of horseshoe crab reagents, and the effects of both methods for detecting endotoxin in the section were within the specified limits. Conclusion After the wet burn cream was dissolved with hexane, it was shaken and mixed with water for bacterial endotoxin examination. It was feasible to take the aqueous phase extract for bacterial endotoxin examination.
3, Colorimetric method
The colorimetric method is based on the hydrolysis of horseshoe crab tripeptide by coagulase activated by bacterial endotoxin, releasing yellow p-nitroaniline, which is detected by detecting the absorbance, or by detecting the red-azo-blue complex formed by the reaction between the released p-nitroaniline and azo reagent.
Although the colorimetric method is more complex, it is more sensitive and accurate and is suitable for endotoxin detection in recombinant protein drugs.
4, Enzyme-linked immunosorbent assay
The enzyme-linked immunosorbent assay quantifies endotoxin by detecting endogenous pyrogens, such as TNF and IL-1, produced by macrophages stimulated by bacterial endotoxin. This method is simple and reproducible, but the operation is tedious, and the sugar
component will stimulate the cells to produce endotoxin to interfere with the detection. The interference of the sugar component must be excluded, and the detection accuracy could be better than the horseshoe crab reagent method.
5, Flow cytometry
Flow cytometry uses monoclonal antibodies against endotoxin surface antigens, fluorescent labeling of endotoxin, and flow cytometry to detect. The method does not need to rely on endotoxin activity and can be directly detected endotoxin content.
Conducting drug impurity analysis, standardized detection, and reasonable control of impurities in drugs is related to the safety and effectiveness of drugs. As one of the critical testing items of drug quality control, the bacterial endotoxin detection method plays a vital role in ensuring the safety of clinical drug use. There are many methods for the detection of endotoxin.
Scientific validation should be performed when determining the detection method, and the verification of the detection method should be gradually improved.
[1] Study of bacterial endotoxin detection method for Cisatracurium besilate injection [J].
[2] A methodological study of bacterial endotoxin examination of burn cream [J].
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