#Electrophoresis (adventure)

[k25ff]

(The) Devouring Hate Ability: haemokinesis, regeneration (primarily of her own blood, secondarily of her bodily tissues), high durability. Side-effect: Changeling-transformative (Echo IV)

Redesigning The Devouring Hate a bit to put more body horror in! Great! (1657)

[kendricklabsinc]

10 Essential Tools for Successful 2D Gel Electrophoresis

2D gel electrophoresis is a powerful technique widely used in proteomics research for separating complex protein mixtures. It allows for the separation of proteins based on their isoelectric point (pI) and molecular weight, providing researchers with valuable information about protein expression patterns and post-translational modifications. To ensure accurate and successful results, it is crucial to utilize the right tools throughout the entire 2D gel electrophoresis workflow. This article will delve into the ten essential tools for successful 2D gel electrophoresis, highlighting their importance in achieving high-quality results and advancing proteomic research.

Introduction to 2D Gel Electrophoresis

Overview of 2D Gel Electrophoresis

2D Gel Electrophoresis is a powerful technique used in molecular biology and proteomics to separate and analyze complex protein mixtures. It involves two distinct separation dimensions: isoelectric focusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In simpler terms, it helps scientists unravel the mysteries of proteins and their functions.

Importance of Using Proper Tools

Just like a chef needs the right knives and pans to create a masterpiece dish, scientists need the proper tools for successful 2D Gel Electrophoresis. Using the right tools not only ensures accurate and reproducible results but also saves time and minimizes frustration. So, let's dive into the essential tools you need for this scientific adventure!

Importance of Using Essential Tools in 2D Gel Electrophoresis

Imagine trying to build a sandcastle without a bucket and shovel. It would be a messy disaster! Similarly, attempting 2D Gel Electrophoresis without the essential tools is a recipe for disaster. These tools provide precision, efficiency, and reliability, allowing you to extract meaningful information from your samples. They make the entire process smoother and increase the chances of obtaining accurate results. So, let's explore the must-have tools that will make your gel electrophoresis journey a success!

Essential Tool 1: Gel Casting Systems

The first essential tool you need in your 2D Gel Electrophoresis arsenal is a reliable gel casting system. This system allows you to create the gel matrix necessary for sample separation. It enables the precise and controlled pouring of acrylamide solutions to form uniform gels. Look for a gel casting system that offers leak-proof sealing, adjustable gel thickness options, and compatibility with different gel sizes. With the right gel casting system, you'll be able to create high-quality gels for optimal separation of your protein samples.

Essential Tool 2: Sample Preparation and Loading Tools

The second essential set of tools you can't do without are those used for sample preparation and loading. Proper sample preparation is crucial for the success of 2D Gel Electrophoresis. You'll need tools like pipettes, microcentrifuges, and protein extraction kits to ensure efficient and accurate sample handling. Additionally, loading your prepared samples onto the gel requires precision and finesse. Consider using specialized pipette tips, gel loading tips, and loading buffers to ensure accurate and consistent sample placement. These tools will streamline your sample preparation and loading, leading to cleaner and more reliable gel results.

Essential Tool 3: Electrophoresis Systems

No, we're not talking about the latest rock band. Electrophoresis systems are the workhorses of 2D gel electrophoresis. These systems provide the platform for separating and analyzing proteins based on their size and charge. It's like a protein beauty pageant, where each protein gets its moment in the spotlight.

Essential Tool 4: Power Supplies and Electrophoresis Units

Let's face it, without power, we're all just sitting ducks. The same goes for 2D gel electrophoresis. Power supplies and electrophoresis units are the backbone of this technique, providing the electrical energy needed to separate those proteins. So, make sure you have a good power supply and electrophoresis unit in your arsenal. Otherwise, your proteins will just be stuck in one place like a stubborn teenager.

Essential Tool 5: Imaging and Detection Systems

You've separated your proteins, but now what? How do you actually see them? That's where imaging and detection systems come in. These nifty tools allow you to visualize and capture images of your precious protein bands. It's like having a protein paparazzi in your lab, capturing all those A-list proteins for you to analyze and admire.

Essential Tool 6: Data Analysis and Interpretation Software

Congratulations, you've got your protein images! Now it's time to make sense of all that data. Enter data analysis and interpretation software. This software helps you analyze and quantify the protein spots on your gel images, giving you important information about protein expression and potential biomarkers. It's like having a protein Sherlock Holmes right at your fingertips, solving mysteries and uncovering hidden clues in your data.

In conclusion, Kendrick Labs, Inc, the successful implementation of 2D gel electrophoresis heavily relies on the utilization of essential tools. From gel casting systems to data analysis software, each tool plays a vital role in ensuring accurate protein separation, detection, and analysis. By investing in these essential tools and staying up to date with advancements in the field, researchers can enhance the quality and efficiency of their 2D gel electrophoresis experiments. Ultimately, these tools contribute to a deeper understanding of protein expression and post-translational modifications, paving the way for advancements in proteomic research and potentially leading to ground-breaking discoveries in various fields of biology and medicine.

Original Sources: https://kendricklabs.blogspot.com/2024/01/10-essential-tools-for-successful-2d.html

[livelectures2]

33+ Fascinating Advanced Higher Biology Project Ideas for Avid Learners

Are you an Advanced Higher Biology student yearning for a meaningful project that not only deepens your understanding but also sets your passion for biology ablaze? You're in the right place! In this blog, we'll take a deep dive into more than 33+ advanced biology project ideas that will transform your journey into an exciting and intellectually rewarding experience.

Genetic Disorders Exploration:

Uncover the intricacies of a specific genetic disorder, unraveling its origins, symptoms, and prospective treatment avenues.

Cancer Biology Investigation:

Venture into the multifaceted realm of cancer biology, probing into the underlying mechanisms and potential therapeutic approaches.

Comparative Anatomy Adventure:

Embark on a journey of comparative anatomy, scrutinizing the anatomical structures of different animal species to unveil their evolutionary connections.

Microbial Marvels:

Dive into the mysterious microbial world as you explore the diversity of microorganisms inhabiting a chosen environment.

Ecological Surveys and Biodiversity Assessment:

Conduct in-depth field surveys to assess the biodiversity and environmental impact of a specific area, potentially identifying ways to protect it.

DNA Fingerprinting Unveiled:

Master the art of DNA fingerprinting and delve into its applications in forensic science.

Human Physiology Deconstructed:

Take a comprehensive journey through the human body, unraveling the intricacies of its various systems and their functions.

Biodiversity Hotspots Expedition:

Investigate a local biodiversity hotspot, focusing on unique species, and contribute to their preservation.

Insect Behavior Observatory:

Immerse yourself in the fascinating world of insects, scrutinizing their behavior, interactions, and ecological roles.

Phylogenetic Tree Construction:

Construct a visual representation of evolutionary relationships among various species, offering insights into the tree of life.

Plant Genetics and Agriculture:

Delve into the world of plant genetics, exploring gene expression and its applications in agriculture and crop improvement.

Endangered Species Conservation Crusade:

Research and advocate for the conservation efforts directed towards safeguarding endangered species and their habitats.

Marine Biology Odyssey:

Dive into the depths of marine ecosystems, discovering the enchanting world of marine life and the challenges it faces.

Animal Behavior Analysis and Communication:

Delve into the captivating world of animal behavior and communication, deciphering their intricate languages.

Immunology and Vaccination Unraveled:

Explore the complex workings of the immune system and the science behind the development of life-saving vaccines.

Molecular Biology Techniques Mastery:

Hone your skills in pivotal molecular biology techniques like PCR, gel electrophoresis, and DNA sequencing.

Neuroscience Exploration:

Plunge into the intricacies of the human brain, investigating its functions and unraveling the mysteries of the mind.

Climate Change's Impact on Ecosystems:

Analyze the profound impact of climate change on diverse ecosystems and their inhabitants, helping raise awareness and inspire change.

Hormonal Regulation and Physiological Balance:

Investigate the role of hormones in maintaining physiological equilibrium and their significance in health and disease.

Cell Signaling Pathways Demystified:

Untangle the intricate web of cellular signaling pathways, understanding their essential role in coordinating cellular activities.

Zoology and Taxonomy Journey:

Embark on an exciting exploration of zoology and taxonomy, unraveling the classification and characteristics of animal species.

Genetically Modified Organisms (GMOs) Saga:

Dive into the GMO debate, dissecting the science behind genetically modified organisms and their impact on agriculture and society.

Biomedical Ethics Expedition:

Tackle complex ethical dilemmas in biomedical research, offering thoughtful insights into the moral compass guiding scientific progress.

Microbiome Mysteries Unveiled:

Unearth the secrets of the human microbiome, exploring its crucial role in health and disease.

Invasive Species Impact Assessment:

Investigate the disruptive impact of invasive species on native ecosystems, presenting strategies for mitigation.

Bioinformatics and Computational Biology Odyssey:

Bridge the gap between biology and computer science, uncovering the pivotal role of computational tools in advancing biological research.

Stem Cell Revolution:

Explore the potential applications and ethical considerations surrounding stem cell research, an area teeming with promise.

Epigenetics and Generational Inheritance:

Delve into the intriguing world of epigenetics and its role in the transmission of traits across generations.

Human Genetic Diversity Exploration:

Embark on a genetic journey, scrutinizing the diversity of human populations and understanding the factors shaping our genetic landscape.

Bioluminescence: Nature's Light Show:

Illuminate the fascinating world of organisms that emit light, exploring the biological mechanisms behind this captivating phenomenon.

Aquaponics Systems: Sustainable Agriculture in Action:

Design and implement an aquaponics system to gain insights into sustainable agriculture practices and the intricate balance of aquatic and plant life.

Biochemistry of Photosynthesis Unveiled:

Dissect the biochemical intricacies of photosynthesis in plants, unraveling the process that powers the plant world.

Bridging Biology and Art:

Marry art and biology to create visually stunning representations of scientific concepts, captivating both scientists and art enthusiasts.

Pharmacology and Drug Development Odyssey:

Journey through the complex process of drug development, from initial discovery to clinical applications, understanding the vital role of pharmacology in healthcare.

Cellular Respiration and Energy Production Expedition:

Journey into the heart of cellular respiration, exploring the intricate processes that generate energy to power life.

Ecosystem Services Investigation:

Uncover the hidden services ecosystems provide to humanity, underscoring the importance of preserving these vital natural systems.

Biology in the Cosmos:

Ponder the challenges and prospects of conducting biological research in space, shedding light on the exciting future of space exploration and biology.

Ornithology and Avian Migration Expedition:

Delve into the avian realm, exploring the wonders of bird species and their fascinating migratory patterns.

Nutrition and Health Nexus:

Investigate the intricate link between nutrition and human health, uncovering the secrets to a healthy, well-balanced life.

With this treasure trove of project ideas, you're now equipped to embark on an incredible journey through the realm of Advanced Higher Biology. Not only will you bolster your knowledge and analytical skills, but you'll also kindle a fervent passion for the intricate tapestry of life on our planet. So, pick a project that resonates with your curiosity and embark on a biology adventure that will redefine your academic experience. Happy researching!

[formula-red]

im here to give you an update on my lab adventures <3

we got to play with fire today which i always love, idk something about the blue flames rushing sounds is just really soothing to me, IM GOING OT BE A MOM SOON, im growing my own bacterial culture only to kill it next wednesday when were going to isolate the plasmids. also our gel electrophoresis when kind of wrong out of 18 samples loaded only 2 showed, two of mine actually so im proud !! why only two showed we dont know so next week we have to do pcr again to hopefully create enough cdna, but as far as i understood everyone in this course is redoing the pcr so im not that worried, the picture of the gel looks kind of sad and im tempted to add it here but idk. of course i got to work with the sybr safe stuff so i looked like a little plant for a bit with my green gloves.

<333

SCIENCE TIME YAY <33333

bacteria mom fin?!?! soon to be muderer fin?!?!?? 😈 yay for your two samples they are so strong 💪💪💪 also if u ever want to send science pics i would love to see 🤓❤️ YAY FOR SCIENCE <3

[gearfalconart]

words & numbers, sound & silence, stop the peace & keep the violence

i’m a huge sucker for nightjar’s design and i had this song stuck in my head two birds with one stone 

[gayrfalcon]

hey so this sure is a fanfic i wrote 

Morning was the worst time of day, Daniel decided, curling up in a vain attempt to go back to sleep. It was barely light outside, the sun just beginning to filter through the windows, but he was already wide awake, the memories from last night shifting to the forefront of his brain.

He burrowed further into the mattress, groan muffled by the covers he pulled over himself. Bonnie was a terrible influence, he had been totally drunk, and there were gonna be videos passed around of him being friendly, and--visibly happy, for months.

Today already sucked, might as well get up. He extracted himself gingerly, trying not to wake the sleeping form beside him. She was usually awake before he was; last night she must have stayed up long after he had crashed, he reasoned. Yawning, he pushed himself off the side of the bed, slipped on a stray sheet corner, and nearly slammed face-first into the floor, telekinetics catching him at the last second and adding a killer headache to his list of hurts in the process. He rolled over mid-air, let himself drop the final inch or so, and lay flat, arm over his eyes. Of course, he thought sarcastically, why would anything go well.

The bed shifted, and he could hear Bonnie stifle a giggle before rolling over to peer down at him. “You good down there?” she asked.

He could hear the smile in her voice. Could picture her posture pretty well without looking, her chin propped on her hand, sleepy-eyed and grinning like a Cheshire cat who caught the canary.

“Fine, except for everything is awful,” he grumbled, ignoring the sudden swell of warmth in his chest.

“What, did you lose control of your--”

“Don’t say it.” “--gross Motor skills?”

He opened his eyes to glare at her, then settled for squinting angrily, eyes unadjusted to the light. “Haven’t I suffered enough.” “Absolutely not. Come on back to bed, it’s barely dawn.”

Mumbling something about I thought you were the morning person here, he hoisted himself back to his feet, wincing as the room spun around him. He crawled back under the blankets and Bonnie curled herself around him, apparently content to rest where she was for once.

Could be worse, he supposed.

[isoscele]

Told you it’d be sporadic. Have an entirely unedited entry for @lumberjanesweek Day Four- AU Day. 

*jazz hands* Roanokes in spaceeeeeee!

Once the airlock is securely shut and the crew has been shuttled off to bed in varying states of complaining exhaustion, Jen whirls on Abigail. “Are you out of your mind?”

Abigail looks like the kind of person whose picture was all over the slideshows in Academy lectures. The horror story--what happens when the siren call of deep space gets strong enough to feel in the backs of your teeth, when you let it take you over. Abigail is waxy from years without any kind of sunlight, whittled out by a lack of light or consistent circadian rhythms. The bags under her eyes have their own gravities. 

“No one told me they were kids,” Abigail says. Her first words since the safety lock failed. The replicator poured her a thermos of tasteless coffee, but her hands are shaking too bad to hold it. “Rosie didn’t--I thought--”

“You know Rosie?”

“In a manner of speaking.” Another sign of spacesickness: her gaze, flitting all around the cramped cabin, unable to rest for more than a few seconds. Scanning for exits.

Jen sighs, and digs in the icebox for something to do with her hands. “My crew is . . . unorthodox.” She has to choose her words carefully. “Sometimes I wish things were different. But you endangered every one of my girls, and you endangered me.”

“I didn’t want to hurt anyone,” Abigail says. “I just--it has to die. Jen? It can’t hurt anyone else.”

Jen just wants to go to bed. “The protocol for unrecorded extraterrestrial beings requires a committee of seasoned graduates to attempt communication. What you did today could get you locked up for the rest of your life.” 

“It hurts people,” Abigail says. “We don’t have time for that bureaucratic bullsh--bull poop, sorry.”

“I’m seventeen,” Jen says, biting down on the familiar exhausted urge to break into laughter. “You can curse around me.”

“The teenage captain of the child crew,” Abigail says quietly. She tries to take a sip of the coffee, winces at the heat. “Jen, is this an adventure story or a tragedy?”

Jen rolls the word tragedy around the edges of her tongue. Abigail, shaky-handed, in Jen’s cockpit, looking for all the world like someone who’s already been chewed up by too many black holes to name. Jen wasn’t supposed to graduate from the Academy for another three years. Most people don’t ask, when they talk to her. “I guess it depends who you ask.”

“Let me guess,” Abigail says. “You tell me if I’m getting it right. Okay?” Without waiting for Jen’s response, she plunges into what is clearly a well-worn narrative. “You’re top of your class at the Academy, but they’ve been pushing back your actual missions for months for reasons you can’t get anyone to tell you. Someone--maybe a friend of a friend, a mentor, even Rosie herself--suggests that you’d be the perfect candidate for the Qiunzella Program, and you sign up because you need the flight hours and it’s not like you’ve got anything else going on. You’re given a skeleton crew of enthusiastic kids whose parents wanted them to have the experience of living on a real certified ship, even if it is the worst bucket of bolts they could find in the hangar. You follow all the programming, meet up three times a day for activities with other ships, keep a constant line of communication with Rosie, and stay well within your boundaries.”

Abigail leans back in her chair, managing to look composed even as it clearly tips her back farther than she expected. “This is the part where I lost the narrative,” she says. “I figure that either something catastrophic happened to pull you off-course, or Rosie didn’t read you the brochure all the way.”

“We’re within boundaries,” Jen says, trying not to chew on the inside of her lip. She knows her tells so much better now, after eight weeks of BS games with Ripley. “Haven’t left the campground.”

“I don’t think it’s your fault,” Abigail says, in a way that makes it very clear whose fault she thinks it is.

“I would know if we left the campground,” Jen says, but she can hear the desperation in her own voice. It was fine when her protegés smuggled some Bellerophonian marsupial on board and started feeding it April’s scrunchies and Jen’s spare AAA batteries. It was fine when they got attacked the first time--surely a simulation--and the second time, and the third time when the hole in the hull was so big that they all might have asphyxiated if Jo wasn’t around. It was even fine when they lost communication with Rosie, when the message boards glitched out one by one until even Zodiac, the last holdout, started sending transmissions that were nothing but forty minutes of static.

They even, by some miracle, got to the other side of today. Even Abigail, with her burnt fingers and twenty years of isolation, is sitting in Jen’s ship and drinking from Jen’s Junior Deluxe Space Camp Plus! thermos and looking ridiculously alive for somebody in her position.

But none of this makes sense because Jen knows how to read the fancy compass attached to the console, and she understands star charts better than half the Academy graduates that go on to be bigshot captains, and she would absolutely notice if they left the campground. They haven’t left the campground.

“There’s nothing here,” Jen says. “There should be nothing here. Like--bacteria and stuff maybe, for collection and recording and teaching the kids how to run a gel electrophoresis and enough satellites to run some of the more complex simulations, but this is--it’s like an amusement park. It’s for kids. It’s safe.”

“They said it was safe when I went, too,” Abigail says. “They’ve always said it was safe.”

Jen grips the edge of the table until her knuckles go white. “What are you saying?”

Abigail takes a long draught of coffee. When she sets the cup down, she looks like a shell of a person, waxed-out and still trembling, head to toe, almost imperceptibly. There’s something in her eyes that reminds Jen of the way she looked, lighting the dynamite, the way her eyes were almost silver in the burn of far-off stars, like little oil spills. She’d been crying, tethered to the hull of her tiny pod by one ankle. 

“You’re right about one thing,” Abigail says. “You’re still in the campground. Everything else . . .?” She spreads out her hands, a sort of forced casualness. “I think you already know.”

Molly’s just going back to her cabin, listening for the slow shh of the automatic doors, when she sees a glint of blue in the shadows behind the kitchen. She stops.

“Rip,” she says softly. “Are you okay?”

Ripley nods. The movement is so jerky that she almost looks like an old stop-motion movie in the buzz of the ever-faulty halogen lights. 

Molly slips out of the hallway. She’s accustomed to moving silently. She wears socks everywhere on the ship, takes each step with a precision that she’s never seen from any of her friends. You can go a galaxy away from home, but something always follows you. She slides an arm around Ripley’s shoulder in a one-armed hug.

“Jen’s talking to Abigail,” Ripley says. Her face, creased with concern, still stained by a black smudge of charcoal. Her teeth are the color of copper nitrate, from the Popsicle she snuck from the cooler when they got back. “I’m making sure Abigail doesn’t explode Jen and try to take over the ship like in Star Jump.”

“Eavesdropping, huh?” Molly draws closer to the door. She can hear Abigail’s voice, the low scratch of it. She can’t hear Jen at all, but there are two silhouettes in the fingernail of light on the tile. 

“Bodyguarding,” Ripley corrects firmly.

“Right.” Molly rubs the inside of her wrists. She can still feel the ghost of the Thing’s claws, cold to the touch and exuding exactly the right amount of pressure to hold her without breaking the skin. Mal disappeared into her cabin two hours ago to write a letter to her mom. Molly can’t imagine finding the words to describe what happened today, can’t imagine letting it cross the threshold of the ship. The fear, and the pain, and the anger burning like nothing else does, in space.

“I made hot milk,” Ripley says, “and I was going to look for the chocolate powder but I got distracted.”

“I think it’s behind the popcorn,” Molly says. “I’ll get it, okay?”

They curl up together, as if conserving heat, in the shadow of the tall block of cabinets. The microwave beeps, quiet as if not to disturb the moment. Molly stirs the cocoa with her finger, and listens for the recurring loop of Abigail’s voice. 

It has the cadence of a story. One they might never hear.

April and Jo lie side by side on Jo’s bed. April traces the careful lettering of her journal, red cover and a going-away present and really, really nice. The pens bleed through every page, each entry scattered with omens of the next one. 

Jo’s half-asleep and trying to keep her ankle elevated. April’s head is on her stomach. They can feel each other’s heartbeats, much too fast and nearly aligned. The old books Jo’s dads tucked into the bottom of her suitcase are half-covered by the sheets, each opened to a different tea-stained page. 

April runs one finger down the margins of the bestiary, and the other across the line of Jo’s scalp. With Jo’s arm wrapped around her, she can almost pretend the bruises on her sternum are merit badges. She flips the page, and stops breathing.

“Grootslang,” she whispers, just to feel the word in her mouth. A rubbery, oiled-down taste. Jo shifts, and opens her eyes, brow tight with inherited fear.

Mal wraps her wrists around her ankles. Touches the tips of her knees with her chin. Catalogs every bit of her body, piece by piece, a framed deconstruction. 

“God dammit,” she whispers. Outside her wide bay windows, planets glitter like shed scales. “God fucking dammit.”

[carlosexpeditions]

Common Tips For Ecological Tour

Eco Jungle Tours are the best place for vacation. Most of families choose this for picnic and adventurous tour. You can stay at Monte Amazonico Lodge with family and the next day you can go to hills, mountains and lake for picnic.

• Guide. It is usually advisable to have a guide. You may not be watching too much wildlife, or you may get injured or lost. Additionally, many countries require local guides when entering national parks. This is not only a service for you; it also justifies the financial park by providing employment opportunities to the locals. In densely populated areas, conservation isn't considered a success without the support of the locals. Locals must reap tangible economic benefits by confiscating routine hunting and subsistence farming activities.

• Homework. To fully understand your rainforest experience, you should learn something before going. It is very useful to have a basic knowledge of the surrounding environment.

• Relax and enjoy. Be careful, though. This will help you find wildlife and have a safer experience.

•Be careful. Look at the fallen leaves and surrounding greenery if you are waiting for someone. You will notice the difference between rainforest and forest ecosystems in North America and Europe. You will be lucky to see small animals hiding among the fallen leaves and leaves if you look closely. Be careful not to trip over the roots or get trapped in the vines when walking. Also, be careful when reaching out to grab a tree trunk for support - thorns are common in the forest. Eco Jungle Tours also helps to know about depth of the nature.

• Silence. Enjoy the sound of the rainforest. Speaking out loud can scare away wildlife and annoy others in your group. Larger groups tend to be smaller animals due to the noise.

• Do not feed wild animals. Feeding wildlife can cause many problems for both you and your pet.

Rainforest Safety Tips

• Listen to the guide. They can understand the rainforest environment better and recognize wildlife signs than you do. Your guide will likely make the most of the wildlife discoveries.

• Encounter with animals. The ones you are most likely to encounter are insects (ants, bees, bees, mosquitoes) and plants (many rainforest plants protect themselves with thorns, thorns, or corrosives. There are many poisonous ants, but your chances are rarely met). During the day. You have to pay close attention to the sunset and the evening.

• Shoes. Please wear comfortable shoes. Small blisters can easily become infected in rainforests and turn into spasmodic wounds. Also, take off your shoes in the morning. Sometimes during the night, scorpions build a house in your shoes.

•   Cover-up. While it can be hot and humid, long sleeves and pants are the best protection against insects, thorny plants, and vines. If you don't want to cover it up, it is advisable to use insect repellent. Sometimes your native guide has some interesting local solutions to keep bugs away.

• swim. In places like the Amazon, there can be problems with wildlife (rays, caimans, piranhas, electrophoresis, and other surprises) and unexpected currents.

• Continue hydration. Drink lots of water - It's easy to get dehydrated in the rainforest. Grumpy is one of the first signs of dehydration, so if someone is particularly frustrated, give them some water.

Health problems

Consult a physician. Ask your doctor to make sure you are in good health for travelling. Tell your doctor where to look for particular concerns and check the CDC Travel website for new health issues in the area.  Some countries require specific immunity to enter the country.

• Fitness. Due to the climate and the lack of general medical facilities, it is necessary to be reasonably healthy before leaving the Amazon and other rainforests. Check with your doctor!

• Travel medical insurance. Find out if your health insurance covers you abroad. If not, if you think you are in danger or want to be safe, take out travel supplemental health insurance.

• Packaging. Put your recipe in your hand luggage. Bring enough for your trip and a little one.

By this you can make your journey joyful and adventurous and come again for making other memories with nature.

[from-a-reckless-writer]

AAAAAAH YESSSS I NEED ALEX AND LENA TO BE SCHEMING IN SCIENCE LABS BECAUSE AS MUCH AS ALEX LOVES THE ADVENTURES SHE AND SUPERGIRL GET UP TO, SHE MISSES THE LAB TOO. SHE DOESN'T EVEN KNOW SHE MISSES BEING A SCIENTIST TILL LENA IS DRAGGING HER TO THE LAB. SHE'S NEVER REALIZED HOW MUCH SHE'S MISSED THE STARING AT WHITE BOARDS FOR HOURS, THE UNMISTAKABLE SMELL OF BURNING HAIR, THE FRUSTRATION WITH A PIPETTE AND GEL ELECTROPHORESIS AND HANGING WITH LENA? HANGING WITH LENA MEANS ALEX GETS TO GO BACK WHERE SHE STARTED, WHAT ONCE WAS HER HOME.

sometimes they spend hours together in the lab and they dont even speak. alex finds out that lena did the same thing she did when she was in college; stay inside the lab to impress her professors and then get shitfaced the next night to forget everything else. She learns that lena knows how to blend in with alex's work habits and they work together effortlessly. the only thing that alerts them that theyve been cooped up inside for so long is Kara barging in and whining about how she cant believe shes lost her sister and girlfren to science. and then Kara realizes wAit im smart as hell I CAN PROLLY HELP WITH ALL THE PHYSICS PLS LET ME SCIENCE WITH U. which of course just leads to kara willingly being their test subject for alien experiments and engineering kryptonian weaponry in between

There's not enough alex/lena brotps, seriously, they would be so good friends. They both like hot women, alcohol, science, and are Kara’s number 1 fans.

I agree!!! I think I have some alex and lena brotp one shots buried in my writing tag somewhere

[claudiogalvin00-blog]

Movie Follows B.C. Skiers' Ecological Journey.

The Bons, Tibet's pre-Buddhist, shamanistic faith, call the hill Tise and also depend on this to become the chair of the Heavens Goddess Sipaimen. I assume our company are going to be actually back at Hill Viewpoint for pick up at 12:30 PM. The gals competition is at 9:00 AM and also the young boys go to 9:40. As opposed to wasting 100s as well as Thousands of dollars journeying throughout the nation on greatest White Mountain bike, it's much better to invest handful of 100 dollars on absolute best White Mountain range shoes also.

The area is offered through a K-12 public school district, phoned the Major Heavens Institution District # 72, and a preK-6 non-profit private school, Big Skies Discovery School. Little Bit Of Bighorn Battleground National Monument, where General Custer made his notorious untoward last position, are some of the major ones.

Twelve countries will be represented in 2013 at Pine Mountain in Iron Mountain MI: USA, Canada, Austria, Estonia, Finland, Germany, Netherlands, Norway, Poland, Romania, Slovenia and also Ukraine. I encountered a condition where I wanted to modify an I.D. code on around 60 approximately containers and containers that I had actually retained at Iron Mountain range. Our team have an attractive lengthy array mountain range perspective from our living-room, room and also back deck. John Dugan is actually a specialist writer which focuses on guys's health and wellness concerns as well as is a continuous providing article writer to various on-line web sites. With going up the mountain range being a highly dangerous undertaking, some resorts in the Amboseli additionally make the most of this limit to outdo Tanzanians. Our team opened five retail stores in the course of the three months ended in August, 3 Cold weather Rock co-branded stores, one residential Rocky Mountain range Chocolate Factory Shop, and one International establishment. IT: Launch Day 2006-01-20 Regarder Brokeback Hill Movie Complet En Francais. When you possess a clear online company strategy, http://smile-behealthy.info on the internet service possibilities accomplish results. Just before you can easily purchase a mtb, you should understand just what kind of using you will certainly be actually performing. Michael Zuidema, a retired Forester off the Michigan Division of Natural Assets (DNR), sent out the bone examples to a creatures laboratory at Colorado State Educational institution's College from Veterinary Medicine and also Biomedical Sciences in Ft Collins, where higher settlement electrophoresis identified this was actually undoubtedly coming from a mountain lion (Zuidema 1999). This is actually mostly because of Madrisa Property, Switzerland's largest adventure park on Klosters' Sonnenberg Mountain. In today times, Team Centrix currently gives exactly what they phone their Daily grind Disobedience website page A vacation to this site as well as you see a list of work-from-home projects they've researched consisting of creating welcoming memory cards as a freelancer. Shimano mountain bike components are a number of the most ideal that amount of money could get and also they really make up 75% of the firm's sales profit. What individuals miss if they perform not go to Table Mountain range is exactly what you don't view on mails.

[netsmp]

Free UDEMY Courses 20 May 2020

New Post has been published on https://netsmp.com/2020/05/20/free-udemy-courses-20-may-2020/

Free UDEMY Courses 20 May 2020

2020 SEO For Beginners: Boost Your Website 2020 Youtube Hack: Paying For Engagement, Not Only Clicks! 3D Painting using FlowScape: Paint Dream Worlds in 3D 8 Fun & Simple Mind Hacks to Sharpen Your Brain ACT PREP MATHS Agile Coach Introducción al Rol Alteryx: Data Science for Non-Scientists Aplos Not for Profit Accounting Software Arduino Automatic Medicine Reminder Arduino Interrupt: Step by Step Guide with Practical Example Artificial Intelligence In Digital Marketing Automatic Irrigation System with Arduino Basic Bookkeeping Hacks Basic Bookkeeping Hacks Biohacking Secrets: Boost Your Physical & Mental Health Blogging For Beginners: Start a Blog Discover Passive Income C Programming Basics For Microcontrollers & Embedded System Cell Structure and Peptides MCQ Practice (Bio Chemistry) Choose the Right Microcontroller ClickBank Affiliate Marketing Secrets Home Business Success Comic Creation for Entrepreneurs Content Marketing Strategy & Techniques: Beginner to Expert Content Marketing Strategy & Techniques: Beginner to Expert Content Samurai: Super High-Speed Video Creation Course ICDL 2019 (Arabic) Creating powerful API's with Django Rest Framework on Heroku CYBER SECURITY SPECIALIZATION : Part 1 Decision Trees, Random Forests, Bagging & XGBoost: R Studio Deep Learning with Keras and Tensorflow in Python and R Design and Simulate Arduino Boards and Test Your Code Digital Marketing (SEO, Google Ads, Google Analytics etc) Digital Marketing (SEO, Google Ads, Google Analytics etc) Discovering Teachers' Innovative Skills Dr. Ahmed AbdelHady - PMP Preparation Course (6th Edition) Easy Breathing, Mindfulness & Meditation Training with Jamie Easy learning C++ for beginners Einstieg ins Online Marketing Emotional Intelligence 2 - Confidence and Career Mastery English Grammar Entrepreneurship 101 - From Idea to Launch (And Beyond) Entrepreneurship Empire: Entrepreneur's Wealth Creation! Enzymes and Antigen MCQ Practice Questions (Bio Chemistry) EQ-2 Resilience and Mental Strength - Emotional Intelligence Excel Analytics: Linear Regression Analysis in MS Excel Excel VLOOKUP for beginners Exploring VirBELA 3D University Campus: Virtual Adventure Fitness Nutrition 101: How to Lose Fat & Build Muscle Fitness Nutrition 101: How to Lose Fat & Build Muscle Fiverr Empire: Kickstart a Fiverr Career & Fiverr Brokering Flipping Mastery - A Guide To Fiverr, Etsy And Ebay Success Freelance Writing Course: 15 Courses in 1 Genetic and Polymerase MCQ Practice Questions (Bio Chem) Glycolysis and ATP Synthesis MCQ Practice (Bio Chemistry) Grant Writing Full Course: Nonprofits, Artists & Freelancers How to build successful project strategy How to Create a Secure Website With WordPress How to Hack WiFi Networks for Beginners How To Rank Videos On YouTube Introduction to Internet of Things(IoT) using Arduino Invade Your Classroom with Digital Robot Teachers Java Programming: Complete Beginner to Advanced Job Costing QuickBooks Online - Contractors Kanban: Productivity & Efficiency for Agile Lean Project Learn Advanced CSS3 Learn Evernote Learn Gmail Learn Google Docs Learn Google Photos Learn how to make Practical PLC project with interfacing Learn Microsoft Excel Learn Microsoft OneDrive Learn Microsoft PowerPoint Learn Microsoft Word Learn React From Scratch Learn to Build Mobile Games using Corona SDK Machine Learning for Beginners-Regression Analysis in Python Make Arduino Board at Home: Step by Step Guide Make Money by Investing in GOLD & GOLD MINING STOCKS Managerial Accounting / Cost Accounting Marketing Analytics: Customer Value and Promotion Strategy Marketing Success With The Power of Social Media Stories Master Arduino without coding Master Financial Reporting Statements and critical Analysis Media Training Public Speaking Training for Candidates Media Training -Radio: How to Speak Effectively on the Radio Membership Site: Membership Recurring Home Business Model Microcontroller Interface with Computer via Serial Port Microsoft Excel - Learn MS EXCEL For DATA Analysis Mommy's Baby Guide: Newborn Bundle of Joy Neural Networks (ANN) using Keras and TensorFlow in Python NLP For Educators Towards Excellence Node.js Crash Course Not for Profit QuickBooks Desktop vs QuickBooks Online PIC Microcontroller Interrupts: Step By Step Power BI Parameters, Templates & Row-Level Security Practical Database Course for Beginners : 6 courses in 1 Practical Java Course: Zero to One Protein Stability and Gel Electrophoresis MCQ (Bio Chem) Public Speaking: You Can be a Great Speaker within 24 Hours QuickBooks Desktop Not for Profit Organizations QuickBooks Online vs Aplos vs Xero Not for Profit Accounting QuickBooks Pro Desktop 2020, 2019, & 2018 Start to Finish RoboAuthor: Content Writing Automation 2020 - Part 4 Sales Skills Training: Give a Winning Sales Presentation Sales Skills: Closing tips to close like a pro Secrets Of Reverse Aging & Longevity Sell Photo Online: Beginners Guide Stock Photography SEO Training 2020 : Complete SEO Guide For Beginners Shopify guide: The complete shopify store creation course Space Render 1.0: Artificial Intelligence in 3D Animation Stop Motion Object Animation Using Davinci Resolve & Bandlab Support Vector Machines in Python - SVM in Python 2019 Taller Forex. Creación de estrategias rentables de inversión Tally ERP 9 with GST - A step by step Mastery Course 2020 Teacher Training: Teachers Can Be Great Speakers Teaching For The New Normal The Art of Multitasking The Beginners Guide to Cyber Security 2020 The Beginner's Guide To Motivation And Meditation The Guide to Freelancing in the Modern Gig Economy The JavaScript Course [May 2020 Edition] The Machine Learning Course 2020 The Modern Myths of Productivity Holding Back Your Success The Node.js Course [May 2020 Edition] The Node.js Course [May 2020 Edition] The Python 3 Course - Learn Python Practically The Python Certification Course The Ultimate Guide To Desander And Desilter-Hydrocyclone Traffic Explosion - Free Methods I Use to Drive Mass Traffic Tricks of the Trade - 12 Great Adobe Premiere Pro Tutorials Ultimate Angular Course - Learn Angular Practically Ultimate Angular Course - Learn Angular Practically Video Editing with Adobe Premiere Pro CC 2020 for Beginners Video Vocab: English Vocabulary for IELTS and TOEFL Viral Content Buzz - Killer Tactics For Blog Promotions 2020 Watercolour Painting - Frequently Asked Questions Webinar Empire: Easiest Way to Deliver your Webinar Content Wedding Speech: You Can Give a Fantastic Wedding Speech Work From Home job learn online teaching nd creating courses Work From Home -Start A Successful Home Business For Seniors Work From Home: The Amazon FBA MasterPlan Writing- The Art of Story Telling Xero Accounting Software 2020 Xero Job Costing – Projects Xero Not for Profit Organization YouTube Marketing Empire: The Blueprint to YouTube Marketing YouTube Marketing For Super Growth: Viralnomics 2020 YouTube SEO Pro [YouTube Search Engine Optimization]

[k25ff]

hey remember the Ability Radar? dont know if anyone dissected it or a reveal was done, so a guess: the exclamation point corresponds to cognitohazardous effects, whereas the lightbulb is to equipment and infrastructure on the scene of the event (or "the theoretical extent of the power's application in respect to user's imagination" ?)

(Meri's referring to this thing)

There's a bunch of things going on here, I think, because the Radar is like, an in-universe rating given by an organisation that is dedicated to giving quick and snappy descriptions to people in, basically, fireteams who are trying to deal with/capture/subdue superhumans. It means a lot of it is subjective, especially [!].

What I will say with regards to your question is that [!] tends to be very high for cognitohazards. But for some of these, it's entirely possible to be marked 'too high' in [!], and [!] is far more subjective than any of the others (lightbulb is probably the second most-subjective).

[bluewizard-gaming]

What's So Best Things About Metal Dice?

Something that I get asked all the time when I tell individuals that Imaginary Adventures sell metal dice is 'the reason metal dice?’

Being an old cap at this RPG thing, the radness of metal dice hop directly out at me... in any case, that probably won't be the situation for everybody. You might be taking a gander at your first arrangement of dice, and spending close $50 is anything but a little speculation. You might be a dice enthusiast who has been utilizing the equivalent for a long time and are watchful about having a go at something new.

 Thus, in this blog passage I want to tell you more about the metal dice that Imaginary Adventures sell, and why they are so marvelous.

 1. How are they made?

Our metal dice are made of a top notch zinc combination, and are thrown utilizing molds. They are totally non-Toxic and conform to worldwide En71 models.

When thrown they are then covered in various manners relying upon the completion - either electroplating for the metallic completions or electrophoresis for the hued variants.

The numbers are additionally indented as a major aspect of the shape and afterward loaded up with paint. This implies despite the fact that the paint may blur after some time, the numbers will consistently be there.

 2. How are they distinctive to ordinary dice?

I'll maintain a strategic distance from the undeniable answer here and state that MAIN contrasts are the weight and nature of the edges.

 Weight

An ordinary plastic 7 arrangement of dice for the most part weighs around 26g. A lot of our metal dice weighs around 122g - almost multiple times as overwhelming!

 For what reason would you need heavier dice? First off they FEEL magnificent.

The additional weight makes a fantastic thump when you roll. This is the most fulfilling piece of utilizing metal dice. They simply feel better to roll. As a general rule, 100% of my dear companions and part individuals who snatched a lot of metal dice never needed to return to rolling plastic.

 Metal dice likewise don't roll as far, so they are incredible in littler spaces (eg. a mousepad on a jam-packed gaming table)

 Edges

The sharpness of kick the bucket edges are essential to helping the dice remain as irregular as could be expected under the circumstances. Investigate the d6's utilized in club - they have the absolute most honed edges around to guarantee the dice roll as arbitrarily as could be expected under the circumstances.

 At the point when plastic dice are made, they are normally placed in a tumbling procedure to clean the dice; however this likewise mollifies the edges. Another lamentable symptom of this is the dice can likewise be made marginally elongated (this applies more to d20s than others), which thusly can influence which numbers appear all the more frequently (consider rolling an egg)

 Not exclusively are the edges of our metal dice fresh (for premium irregularity!) they additionally aren't put through any procedures like tumbling. Each incredible sell is made utilizing a similar form, and consistently turns out molded precisely as proposed.

 If you are looking to buy the best metal dice then visit bluewizardgaming.com

[davidacespedesv]

This is my linocut portrait of Canadian medical researcher Maud Menten (1870-1960), who has been called the grandmother of biochemistry and a radical feminist 1920s flapper, and a petite dynamo. Each print is 9.25 by 12.5 printed by hand from two blocks on Japanese kozo (or mulberry) paper. This https://etsy.me/2V91egX

[terrancepapathan-blog]

Hill Dew's New Consume DEWShine Looks Like Moonshine, Tastes Like Soda.

KUALA LUMPUR (Reuters) - The bodies of 11 climbers have been actually recuperated coming from Malaysia's greatest top Mount Kinabalu after a 6.0 enormity quake activated a collection of landslides, authorities mentioned on Saturday, as rescuers looked for eight still skipping. Cougar in Tucson made use of woodland and also stayed clear of urban and also agriculture gardens (- NlnLAMBDA.sub.8 = 0.028, P This all resulted in a bottom line derivable to shareholders for the 3 months finished June 30, 2015 of 1.6 thousand or minus $0.01 each reveal as compared to take-home pay from 9.5 thousand or even $0.08 every share for the three months finished June 30, 2014. Some of the key ramifications this breakthrough is that is actually exposes a relationship in between temperature as well as mountain building. Environment-friendly Mountain range has actually seen lowering revenue growth, off having ONE HUNDRED% profits development just a handful of years earlier. Nonetheless, for numerous western side individuals, Yellow Mountain range may seem not familiar with all of them. After a last Training class 2 stone wall structure (concerning 50' of scrambling), you will discover your own self on the summit from Cony Mountain. When a 19 year aged begged guilty of very first level murder when he eliminated his friend at the falls, one more certain happening happened in 1994. The tale from the supernatural fight was only some of her supernatural true tales health-and-fit.pt off her opportunity on South Mountain range. Opportunity for Eric as well as his accomplices, which were climbing under the Mountain range Insanity flag, to complete their goal was actually quickly ending.

[k25ff]

The face is another mask, a mask that hides a nightmare of twisting crimson.

The Bonechild confirmed for magical girlthing. Get yourself a girl who peels. (1521)