#Covid-19 antigen test in gurgaon | Explore Tumblr posts and blogs

houseofdiagnostics · 3 years ago

Text

RT-PCR test centres in Gurugaon- House of Diagnostics(HOD)

The Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a sensitive and reliable molecular test that detects the presence of genetic material from a specific organism, such as a virus. PCR tests are used to screen the presence of viral RNA, by targeting one or more gene fragments. The test can detect whether or not the someone has the virus very early in the course of the disease.

Converts RNA into DNA

In an RT-PCR test, RNA is extracted from the swab collected from the patient. The RNA is first transcribed into complementary DNA (cDNA) by reverse transcribing of the RNA templates using the reverse transcriptase enzyme. The PCR process then amplifies a particular DNA fragement (the sequence of interest, DNA of interest, or target DNA) into millions of copies. This improves the ability of the test to detect the presence of virus. It is only after multiple cycles that a detectable amount of virus is produced. The technique helps detect a particular RNA sequence with a high degree of accuracy and sensitivity. Since this process involves an in vitro technique, the diagnosis of coronavirus has now become easier.

Accuracy

People with suspected COVID-19 infection have to know their infection status as early as possible, so that they can self-isolate, receive treatment, and inform close contacts. As compared other tests such as the antigen and antibody tests, the RT-PCR tests are considered to be far more accurate. However, in a large sample size, there is a possibility that the test can detect some false-negative patients due to errors in sample collection, processing or transport. With most COVID-19 cases occuring within 3-5 days after exposure, the RT-PCR test must be conducted eight days after suspected exposure or infection, to ensure that enough viral material is present to detect.

Importance of Ct value

Ct value is the short form of cycle threshold that comes out during the RT-PCR tests. As per the ICMR advisory, the Ct value in an RT-PCR reaction refers to the number of cycles after which the virus can be detected. While a low Ct value indicates that the viral load was higher, a higher Ct value shows that the viral genomic load was low. According to ICMR, a patient is considered COVID positive, if the Ct value is below 35. A patient is said to be negative if the Ct value is above 35.

When to go for a RT-PCR test

You came into close contact with someone who has tested positive for the virus

You work in a healthcare facility or are healthcare provider

Need to be cleared for travel or work

You are in a high risk group prioritised for coronavirus treatments

You live or work in facilities such as nursing homes

How is the test administered?

For a RT-PCR test, a doctor or a nurse uses a cotton-tipped plastic swab to collect the sample from the throat or nose. For instance, a nasal swab is inserted two inches into your nose and rotated gently for a few seconds. The swab is then removed and put inside in a tube to be sent to a lab for analysis. The test can be uncomfortable but not overly painful.

There are a number of RT-PCR test centres in Gurgaon that can give you accurate and precise results. Regarding RT-PCR test cost in Gurugram, the government has standardised the price of RT-PCR tests.

#RTPCRTestCentresInGurgaon #rtpcrtestdelhi

0 notes

moderndiagnostic · 4 years ago

Text

RT PCR Covid 19 Test in Gurgaon

The scale and precision of progressing COVID-19 testing in India are in a continuous condition of development, with better than ever units created by biotech organisations overall appearing. As of late, the Indian Council of Medical Research suggested ELISA (short for 'enzyme-linked immunosorbent assay') for labs to test for COVID-19.

ELISA falls under one of two general classifications of testing being utilized by each nation around the globe to keep steady over their pandemic control:

Neutralizer testing (a.k.a serological testing)

Antigen tests

Polymerase Chain Reaction-based tests (like RT-PCR and TrueNat)

While some are more solid than others, no single test is 100 percent exact. There are key contrasts between them – in the manner they work, yet besides their favorable circumstances and constraints, which are one of a kind to each.

How the RT PCR COVID-19 Test In Gurgaon works:

Antibody testing (or serological testing) is an assessment of proteins in the circulation system or bloodstream, to see if an individual has been tainted with COVID-19. A tainted individual will have explicit antibodies to microorganisms they have been presented to. The immune system produces antibodies as a feature of a bigger procedure to safeguard itself from the disease.

All things considered, antibodies tests will most likely be unable to show whether the infection is at present tainting the body. Not at all like a nasal or throat swab test, has searched for hereditary marks of the infection in the body, counteracting agent test searches for hints of the body's reaction to the infection.

Antibodies are plentiful in the blood, so a sample of blood is gathered by either a finger prick or a blood test drawn with a needle. Two explicit antibody types are searched out in an immunizer test:

IgM antibodies against SARS-CoV-2, which grows at an early stage in a disease.

IgG antibodies against SARS-CoV-2, which are for the most part found after somebody has recouped from the contamination.

While there still are many developments taking place regarding the RT PCR COVID-19 Testing, we still have specific tests available to us. We can be certain of our health reports by taking the RT PCR COVID-19 Test In Gurgaon at Modern Diagnostic!

For more information on RT PCR COVID-19 Test In Gurgaon, contact Modern Diagnostic!

#rt pcr test #covid 19 test

0 notes

biotechtimes · 5 years ago

Text

Graduate Aptitude Test - Biotechnology (GAT-B) 2020 Notification

New Post has been published on https://biotechtimes.org/2020/05/31/graduate-aptitude-test-biotechnology-gat-b-2020-notification/

Graduate Aptitude Test - Biotechnology (GAT-B) 2020 Notification

REGIONAL CENTRE FOR BIOTECHNOLOGY An Institution of National Importance Established by the Department of Biotechnology, Government of India, under the auspices of UNESCO Faridabad-Gurgaon Expressway, Faridabad -121001

Regional Centre for Biotechnology is conducting the Graduate Aptitude Test-Biotechnology (GAT-B), an eligibility test for admission to the DBT-supported PG programmes in Biotechnology in participating institutions.

This test replaces Combined Entrance Examination in Biotechnology (CEEB), conducted previously by JNU Based on the examination score, an All-India category-wise merit list with GAT-B rank will be generated. Using the rank, the candidate may apply to various DBT-supported PG programmes in participating institutions. The participating institutions will advertise these programmes and admit students as per their admission & selection process.

Applicable test fee

General and OBC category – Rs. 1000/-

SC, ST, EWS and Differentially abled – Rs. 500/-

Graduate Aptitude Test – Biotechnology (GAT-B) Syllabus

The examination will be conducted in a single shift for a duration of 180 minutes. The question paper will have two parts:

PART- A: Part A will have 60 compulsory multiple-choice questions of the level of 10+2 in the subjects: Physics, Chemistry, Mathematics and Biology. Each correct answer will be of one mark each. There will be negative marking and for each wrong answer, ½ (half) mark will be deducted.

PART-B: Part B will have multiple choice questions of bachelor’s level requiring thinking and analysis. There will be questions from basic Biology, Life Sciences, Biotechnology and allied areas as per the syllabus given below. There will be 100 questions out of which the candidates will have to attempt 60 questions. Each correct answer will have a weightage of 3 marks. There will be negative marking and for each wrong answer, one mark will be deducted.

PART-B Syllabus

General Biotechnology:

Biochemistry: Biomolecules-structure and functions; Biological membranes, structure, action potential and transport processes; Enzymes- classification, kinetics and mechanism of action; Basic concepts and designs of metabolism (carbohydrates, lipids, amino acids and nucleic acids) photosynthesis, respiration and electron transport chain; Bioenergetics

Microbiology: Viruses- structure and classification; Microbial classification and diversity (bacterial, algal and fungal); Methods in microbiology; microbial growth and nutrition; Aerobic and anaerobic respiration; Nitrogen fixation; Microbial diseases and host-pathogen interaction

Cell Biology: Prokaryotic and eukaryotic cell structure; Cell cycle and cell growth control; Cell-Cell communication, Cell signalling and signal transduction.

Molecular Biology and Genetics: Molecular structure of genes and chromosomes; Mutations and mutagenesis; Nucleic acid replication, transcription, translation and their regulatory mechanisms in prokaryotes and eukaryotes; Mendelian inheritance; Gene interaction; Complementation; Linkage, recombination and chromosome mapping; Extra chromosomal inheritance; Microbial genetics (plasmids, transformation, transduction, conjugation); Horizontal gene transfer and Transposable elements; RNA interference; DNA damage and repair; Chromosomal variation; Molecular basis of genetic diseases.

Analytical Techniques: Principles of microscopy-light, electron, fluorescent and confocal; Centrifugation- high speed and ultra; Principles of spectroscopy-UV, visible, CD, IR, FTIR, Raman, MS, NMR; Principles of chromatography- ion exchange, gel filtration, hydrophobic interaction, affinity, GC, HPLC, FPLC; Electrophoresis; Microarray.

Immunology: History of Immunology; Innate, humoral and cell-mediated immunity; Antigen; Antibody structure and function; Molecular basis of antibody diversity; Synthesis of antibody and secretion; Antigen-antibody reaction; Complement; Primary and secondary lymphoid organ; B and T cells and macrophages; Major histocompatibility complex (MHC); Antigen processing and presentation; Polyclonal and monoclonal antibody; Regulation of immune response; Immune tolerance; Hypersensitivity; Autoimmunity; Graft versus host reaction.

Bioinformatics

Major bioinformatics resources and search tools; Sequence and structure databases; Sequence analysis (biomolecular sequence file formats, scoring matrices, sequence alignment, phylogeny); Data mining and analytical tools for genomic and proteomic studies; Molecular dynamics and simulations (basic concepts including force fields, protein-protein, protein-nucleic acid, protein-ligand interaction)

Recombinant DNA Technology

Restriction and modification enzymes; Vectors; plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome; mammalian and plant expression vectors; cDNA and genomic DNA library; Gene isolation, cloning and expression; Transposons and gene targeting; DNA labeling; DNA sequencing; Polymerase chain reactions; DNA fingerprinting; Southern and northern blotting; In- situ hybridization; RAPD, RFLP; Site-directed mutagenesis; Gene transfer technologies; Gene therapy

Plant and Animal Biotechnology

Totipotency; Regeneration of plants; Plant growth regulators and elicitors; Tissue culture and Cell suspension culture system: methodology, kinetics of growth and, nutrient optimization; Production of secondary metabolites by plant suspension cultures; Hairy root culture; transgenic plants; Plant products of industrial importance.

Animal cell culture; media composition and growth conditions; Animal cell and tissue preservation; Anchorage and non-anchorage dependent cell culture; Kinetics of cell growth; Micro & macro-carrier culture; Hybridoma technology; Stem cell technology; Animal cloning; Transgenic animals

Bioprocess Engineering and Process Biotechnology

Chemical engineering principles applied to biological system, Principle of reactor design, ideal and non- ideal multiphase bioreactors, mass and heat transfer; Rheology of fermentation fluids, Aeration and agitation; Media formulation and optimization; Kinetics of microbial growth, substrate utilization and product formation; Sterilization of air and media; Batch, fed-batch and continuous processes; Various types of microbial and enzyme reactors; Instrumentation control and optimization; Unit operations in solid-liquid separation and liquid-liquid extraction; Process scale-up, economics and feasibility analysis.

Engineering principle of bioprocessing – Upstream production and downstream; Bioprocess design and development from lab to industrial scale; Microbial, animal and plant cell culture platforms; Production of biomass and primary/secondary metabolites; Biofuels, Bioplastics, industrial enzymes, antibiotics; Large scale production and purification of recombinant proteins; Industrial application of chromatographic and membrane-based bioseparation methods; Immobilization of biocatalysts (enzymes and cells) for bioconversion processes; Bioremediation-Aerobic and anaerobic processes for stabilization of solid / liquid wastes

Instructions for Graduate Aptitude Test – Biotechnology (GAT-B)

Please submit only one application form with valid details. Do not submit dummy applications.

Keep a copy of submitted online application for future reference. Visit RCB website (www.rcb.res.in/GATB) from time to time for any further information.

A candidate who has either appeared or is due to appear in the final examination of their qualifying degree is eligible to apply, under the result awaited (RA) category. Such candidates will have to submit the attestation form duly certified by the Head of the Institute with stamp (with address and name) from where the candidate is appearing for the qualifying degree. In case the candidate cannot furnish the certificate due to the COVID-19 situation, such candidates may submit the undertaking given on the website

Candidates are advised to download and use the same format/s for relaxation certificates/ attestation format as provided on the homepage of the application portal.

To avoid the last-minute rush, candidates are advised to apply early. RCB will not be responsible for connectivity issues or any other problems at the last minute.

Please go through the detailed notification on the RCB website. The candidate must check the eligibility criteria before applying for the test.

Candidates should preview their application form before the final submission. NO request for a change in the application form/test centre will be entertained after the final submission of the online application.

In case of non-payment of the application fee, the candidate will not be allowed to appear for the test.

All necessary intimations will be sent via E-mail/SMS only to the registered email id and mobile number. So please provide a valid email id and working mobile no. Candidates are advised to visit the website for any new information/ announcement.

The candidates are advised to carefully read the above instructions.

Check eligibility criteria

How to apply for Graduate Aptitude Test – Biotechnology (GAT-B)

1. You can register for GAT-B from the official RCB website http://www.rcb.res.in/GATB 2. Steps for Registration are as follows:

Submit Application, process payment.

Upon creating a profile, you will receive a User ID and Password on your registered email ID and mobile number. Using these credentials, you can log in as a Registered Applicant to fill the Application form. The application may be saved at any stage and can be re-visited to complete later. Please note once the form is submitted, you will not be allowed to edit any details in the application form and application fee paid will not be refunded.

Candidates must fill the application form carefully, once submitted after payment of requisite fee, no edits/ modifications will be permitted.

Important Dates

Online Registration Start Date 01st June 2020 Online Registration Close 18th June 2020 Issue of Admit Card 25th June 2020 GAT-B 2020 30th June 2020 Display of Question Paper and Answer Key on website 02nd July 2020 Last date of accepting representation of any discrepancy in Question Paper & Answer Key 06th July 2020 Declaration of GAT-B rank of Candidates 20th July 2020

#Admission #M.Tech Admissions #MSc Admission

0 notes