#CCL12

[magnoliasinbloom]

Crash Course Love

Infinite thanks to @anna-swims​ and @lcbeauchampoftarth​ for being awesome betas.

AO3 :: Previously

12: Past Tense [Claire]

I’d been dreaming of Jamie.

I had dreamed of his hands roving all over me, touching me, pleasuring me. I thought I had dreamt his hand on my breast, his arousal pressed against my bum, and I shamelessly ground my body on his, in my lust-fueled dream. The sound of his voice had hit me and it had stopped being a dream.

I’d made it become reality. I’d gone for broke and kissed him, and more. What on earth had possessed me to do that?

You’re insanely attracted to him, that’s why.

He hadn’t rejected me, and for that I was grateful. But now paranoia had set in and I was worried about what our encounter would do to our budding friendship. Afterwards, I had felt a little stilted and awkward. He gave no outward sign of discomfort, but was attentive and polite as usual.

But now that I knew what Jamie looked like in the throes of passion, starting a conversation became doubly hard. The roads had been cleared, the snow storm having spent itself in a night. After breakfast with his family and being hugged goodbye by everyone (including my vague promise to Ellen about coming back soon), he had driven me home; the radio was on a little bit loud, breaking up the silence between us. We managed a few half-smiles, a brush of hands here and there, and a promise to call each other soon. We had a wedding to attend, after all.

I had a few texts from Louise and a voicemail, who wanted to go over the flower arrangements one final time, now that the wedding invitations had been mailed and RSVPs were pouring in, including mine. The wedding was set in a few weeks, right before Christmas. The shop was closed on Mondays, but I texted her back so we could meet up later that week. I did a load of laundry. I went over some invoices for the shop. And all the while, in the back of my head, the memory of Jamie’s mouth and hands on me lingered.

The ringing of my phone startled me out of my reverie; Jamie Fraser flashed on the screen, and my heart pounded in double-time. The tension in my shoulders eased and I felt something unclench in my stomach I hadn’t even realized was there.

“Hello?”

“Hello, Sassenach, it’s Jamie. Well, o’ course ye ken that, mobiles show ye who’s calling, don’t they. But why do we always answer the phone like we dinna ken who’s calling, right?”

“Hi, Jamie. You know, you’re right about that. I’ll start answering my phone differently from now on.” I laughed, set further at ease by the Scottish burr of his voice.

“Och, weel, I just wanted to thank ye for accompanying me to lunch. And being so nice to my family. They absolutely loved ye, I think ye could tell. And I wanted to say… sorry. I guess. For the… this morning, ye ken. In case ye were regretting it. Or if ye think I was out of line.”

“Actually, Jamie, I was hoping you didn’t think I was out of line.” My hands fiddled with the papers on the table. “I think I was pretty clear about what I wanted. But maybe you didn’t want to be pawed at and I don’t want you to think that it’s all I wanted from you. You’re my friend, and I wouldn’t want this to come between us.”

“Friend?” Jamie repeated.

“Of course, I consider you my friend,” I said, confused. “Aren’t we friends?”

“Aye, of course, Claire.” He paused. “There was one more thing I wanted to ask ye. As friends, then.”

“Sure.”

“I meant to ask ye out. On a proper date.” Jamie’s tone went up on the last word, making it sound like a question.

“A date.”

“A real one. Not just coffee—unless that’s what ye would like, of course. But I thought perhaps dinner.”

“Yes.” I didn’t hesitate any longer. My fingers gripped the phone tightly, and the swooping feeling of butterflies was back in my stomach, but for a good reason.

“Really?” Jamie asked, incredulous.

I laughed again. “Yes, Jamie, I’m saying yes. Would this Friday be alright?”

“Sounds perfect. Shall I pick ye up at 7? Did ye have anything in mind that ye’d like?”

“Whatever you choose will be fine. I trust you.”

He didn’t know how much.

- - -

For the rest of the week, I spent my days dreaming about my date with Jamie. Date, date, date. A real date. I put in flower orders for bouquets and tended to the indoor plant boxes that held rosemary, parsley, and thyme, but all the while my thoughts were with Jamie.

After meeting Louise on Friday morning for some final wedding details, I left the shop in a hurry, already planning my outfit in my head. Dress up, or seem casual? Maybe a mix of both? As I ransacked my closet, pulling out shirts and jeans and the few dresses I owned, I decided to call Geillis.

“I have a date tonight.” I didn’t even bother to say hello as soon as she answered.

“Ye do?” Geillis Duncan was one of the few people in Glasgow who’d made Frank and me feel welcome back when we were new to the city. She owned a small but popular café near the flower shop. Our friendship had survived my breakup; it dawned on me that we hadn’t talked to each other in a couple of weeks, and she knew nothing about Jamie. I filled her in on some of the details, keeping the most recent private ones to myself.

“So he’s picking me up in like, an hour, and I don’t know what to wear!” I wailed, trying to zip up the back of a dress and giving up in frustration.

“It sounds like ye’re overthinking this, Beauchamp,” Geillis said. “Why don’t I come over now and lend ye my black skirt ye like so much and the yellow top? It’ll bring out the color of yer eyes, I’m sure Jamie will love ye in it.” She was giggling madly at the idea.

“Don’t tease me, Geil, I’m so not in the mood right now. But thank you.”

We hung up, and twenty minutes later she was at my door, helping me with my hair and make-up after I had dressed. I knew there was an ulterior motive to her being at my flat, and she confirmed in no uncertain terms that she wanted to see Jamie herself.

“Geillis, please don’t—”

“Relax, Claire. I just want to see the lad’s whose bonny red hair has ye in such a fluster.”

“You have red hair, you know.”

Geillis clucked. “’Tis not the same, and ye ken it. When will he be here?”

Before I could respond, there was a knock at the front door. It was promptly seven o’clock, and I glanced at Geillis in a panic. She smoothed down the skirt and pushed me towards the entrance. Heart pounding, very much aware that Geillis was peering gleefully around the hall for a glimpse of Jamie, I opened the door to find a very dapper Jamie. In dark jeans, a pressed sky-blue shirt and a black coat. The hues of his outfit brought out the intense sapphire of his eyes as his own gaze raked me over and seemed please at what he saw. I blushed.

“Hello, Sassenach.” He leaned in to kiss my cheek and his fingers lingered briefly on my arm. I caught the scent of his cologne, like tart lemons and spice.

“Hi, Jamie.” We stood there for a few seconds that seemed an eternity, before a loud harrumph and a fake cough from Geillis broke us out of our reverie. Jamie peered into the flat as I quickly grabbed my purse from the kitchen table where I’d left it before.

“Is there someone here with ye, Sassenach?”

“It’s my friend Geillis, but don’t worry, you don’t need to meet her and she was just leaving. Weren’t you, Geil?” I raised my voice for her benefit as I led Jamie out of the flat. “Lock up when you go!” I shut the door on one of her loud laughs; I was sure to hear from her later.

We walked to the stairs and Jamie tentatively reached for my hand. I grasped it firmly and squeezed in reassurance. Traipsing down the stairs, and remembering the last time we had done so together, I felt stupidly happy and shy all at once.

The restaurant he’d chosen was a low-key pub tucked into one of Glasgow’s winding alleys. We ordered wine and the awkwardness that I’d feared after our previous encounter was gone. Jamie and I talked animatedly about our week; my preparations for the upcoming wedding and flower arrangements, and he told me of the distillery and all the Christmas orders they had to fill.

“I was thinking of a new special blend; aging whisky in tequila barrels, not regular oak. The flavor is more complex, so different from what I’ve tasted. I plan to call it something like da anam, two souls.”

“That sounds very different! Where would you get the barrels?”

Jamie spoke of partnering up with several tequila producers in Mexico, as I speared rosemary potatoes with my fork; all the while we poured glass after glass of ruby wine for each other. Conversation flowed between us just as effortlessly.

Over dessert sometime later, I felt the back of my neck prickling. I sensed eyes on me, and they weren’t Jamie’s. It felt wrong, somehow.

I turned my head slightly and found Frank looking at me. He was with Sandy; he quickly bowed his head and shifted his attention elsewhere. I felt my face flush. I swiveled back and dropped my fork with a clatter.

“Sassenach? What’s wrong?”

“I don’t—it-it’s Frank.”

“Where?” He looked around wildly and I shushed him and clamped my hands on his arm in desperation.

“He’s back there, with the blonde. Just—be discreet.” Jamie got a good long look and then leaned in to whisper quietly.

“Didn’t ye say Sandy—his fiancée, with the giant ring ye mentioned. Wasn’t she supposed to be pregnant?”

“She might have had the baby, I don’t know. Her stomach was pretty big last time I saw her.” I sneaked another look.

It wasn’t Sandy.

She was blonde like Frank’s fiancée, but this wasn’t her. She looked even younger, fresher-faced, and was definitely not pregnant.

Cheating, lying, bastard.

I took deep breaths and Jamie ran a hand soothingly down my back. I shivered and grabbed my coat off the back of my chair.

“Jamie, I’m sorry, can we go?”

���Of course, Sassenach.” After quickly settling the check, he stood up as unobtrusively as a six-foot man ever could and pulled out my chair. He put his arm around me as we walked quickly to the exit.

It was inevitable that we pass by Frank’s table, though. The restaurant was a bit crowded and the layout made it impossible to avoid him. As we did, I got up the courage to meet his eye, bolstered by Jamie’s warm hand on my back. He wore a shamed expression, and could not hold my gaze. The woman stared back curiously at us, and I heard her ask him who I was.

“No one,” Frank replied, a slight tremor in his voice. Jamie tightened his grip on me, and I knew he’d heard him too.

Jamie came to a sudden halt near their table; he turned to face me, and with a soft whispered, “I hope ye dinna mind this,” pressed a soft kiss to my pursed lips. I opened my mouth in surprise, and he continued to probe gently. I found my arms rising to encircle his waist, clutching at the back of his coat. I dimly heard Frank clear his throat and murmur something unintelligible. I had ceased to care, though, lost in the fog of kissing Jamie.

Jamie’s mouth trailed across my cheek. “Dinna listen to him, Sassenach,” he whispered as he nuzzled my ear. “Ye’re so much more than ye know.”

- - -

A/N: I finished writing it out, so new chapters will post on Thursday. Finally, a schedule! The whisky in tequila barrels is actually a thing. Can’t find an English link, though. Thanks for all your likes, reblogs, comments. <3

[savvyingthesignals]

Our computer laboratory for the subject: CCL12, Department of Information Technology 😊💻

[healthtimetaylor]

Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection.

PMID:  BMC Complement Altern Med. 2019 Feb 11 ;19(1):44. Epub 2019 Feb 11. PMID: 30744623 Abstract Title:  Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in AG129 mice. Abstract:  BACKGROUND: Carica papaya leaves have been used for traditional treatment of dengue fever and have been reported to exhibit an immunomodulatory activity by affecting the level of cytokine production in vitro and in vivo. Due to the lack of adequate in vivo evidence in dengue disease model, the present study was initiated to screen and identify the cytokines affected by freeze-dried C. papaya leaf juice (FCPLJ) treatment in AG129 mice infected with DEN-2 dengue virus.METHODS: The AG129 mice were fed orally with FCPLJ for 3 consecutive days after 24 h of dengue virus inoculation. Plasma cytokines were screened by using ProcartaPlex immunoassay. The gene expression in the liver was analyzed by using RTProfiler PCR Array.RESULTS: The results showed that FCPLJ treatment has increased the plasma CCL2/MCP-1 level during peak of viremia. Gene expression study has identified 8 inflammatory cytokine genes which were downregulated in the liver of infected AG129 mice treated with FCPLJ. The downregulated inflammatory cytokine genes were CCL6/MRP-1, CCL8/MCP-2, CCL12/MCP-5, CCL17/TARC, IL1R1, IL1RN/IL1Ra, NAMPT/PBEF1 and PF4/CXCL4.CONCLUSION: The findings indicated the possible immunomodulatory role of FCPLJ during dengue virus infection in AG129 mice.

read more

[longevime]

Neurogenesis

Video: You can grow new brain cells. Here’s how | Sandrine Thuret

neurogenesis - grow new brain cells

Hippocampus can generate new neurons - important for learning, memory, and spatial recognition

Seen that depressed people have lower levels of neurogenesis and when given antidepressants, see more neurogenesis

If block neurogenesis, block efficacy of antidepressants

Environment can have impact: learning, running, sex increase neurogenesis; stress, lack of sleep, aging decrease neurogenesis

Foods that increase neurogenesis: resveratrol, omega 3 fatty acids, caloric restriction, blueberries, folic acid, zinc, flavonoids, curcurmin, caffeine, intermittent fasting

Foods that decrease neurogenesis: vitamin E, B, A deficiency, high sugar, high saturated fat, ethanol, soft diet (chewing helps neurogenesis)

Studies in mice

Video: The brain may be able to repair itself - with help | Jocelyne Bloch

Doublecortin-positive cells - led to neural stem cells; 4% of brain and responsible for embryonic brain development (but why stay around in adult?)

Cultured and reimplanted into normal brain and cells disappeared after a few days

If reimplanted into damaged brain, remains and become integrated as neurons

Monkey studies (stroke model)

Wikipedia: Adult neurogenesis

increase in neurogenesis improves cognition; new neurons increase memory capacity, decrease interference between memories, add info about time to memories

Inconclusive relationship to Alzheimer’s

Decrease neurogenesis in depression may be linked to increase stress hormone glucocorticoids

Applications in Parkinson’s and traumatic brain injury

Alpha7 nicotinic acetylcholine receptor agonist PNU-282987 - had follow up that it increased neurogenesis

Exercise-induced neurogenesis due to brain-derived neurotrophic factor (BDNF) and IGF1; BDNF increases production; IGF1 increases uptake

Exercised mice that did not produce beta-endorphin had no change in neurogenesis

Synthetic cannabinoids increase growth of new cells, protect against brain inflammation and might result in better memory at old age

Cannabidiol increases neurogenesis, but no effect on learning

THC impairs learning and no neurogenesis

Mice plasma chemokines correlate with reduced neurogenesis: CCL11, CCL2, CCL12; may be genetic locus in aging

Epigenetic regulation and DNA methylation might contribute via Dix2, Neurog2, Sp8; microRNAs (miR-124, miR-9) influence cortical size and layering during development

[aradanasraymond-blog]

It's my Senior Year.

Another semester full of challenges.Many hours of sleepless nights to come. Hundreds of examinations to take. Numerous requirements to accomplish. It completely runs my mind as I enter my senior year being an ECE student.

On the eight of august 2017, second day of my schooling of the semester. Tuesday. I have my 7 am class. ECEN 70-Signals, Spectra and Signal Processing. Since this is the first meeting of the course, I don't feel any pressure at all.

The first day of my class in this subject, I came late. Since this is the first meeting in the subject, I have no idea where will our classroom be. I'm lost, so I texted some of my classmates. And someone replied "Dito kami sa DIT, CCL12 first floor." When I learned about the room, I walk faster. Fortunately, I feel relieved as I see one of my classmates and shouting "Momon, late ka rin?" We both feel nervous thinking about what sir Arboleda will tell us. As we enter the room, unexpectedly he surprised us by giving a big smile. We are comfortable now to take our seats.

He continue the discussion. And upon learning that I missed the earlier discussion about the subject, I asked some information about what sir Arboleda discussed when I was not on the room. I wrote it on my notebook and I started listening to him.

After the whole discussion, I am challenged. The subject requires a lot of effort and focus. I swear that this first blog will be the beginning of this journey to the world of Signals, Spectra and Signal Processing.

[cancersfakianakis1]

Peripheral viral challenge triggers hippocampal production of inflammatory proteins

Abstract

Peripheral viral infections increase seizure propensity and intensity in susceptible individuals. We have modeled this comorbidity by demonstrating that intraperitoneal (ip) injection of the conventional viral mimetic, polyinosinic-polycytidylic acid (PIC), renders the brain hypersusceptible to seizures induced by kainic acid (KA). At the molecular level, the hippocampus, which is the ictal site of KA-induced seizures, exhibits upregulated expression of messages encoding several inflammatory genes. Here, we profiled temporal expression of these genes at the protein level. Briefly, eight-week old female C57BL/6 mice were ip injected with 12 mg/kg of PIC and inflammatory proteins were quantified in the hippocampus and blood by ELISA. We found a robust but transient increase in blood concentration of IL-6, CXCL10, CCL2, CXCL9, CCL7 and CCL12 six hours after PIC challenge. CXCL1, IL1β, TNFα and CXCL2 featured a moderate increase. However, only four chemokines were increased in the hippocampus. CXCL10 showed the highest increase 6–12 h after PIC challenge, and its level dwindled to the baseline by 48 h. CXCL1, CXCl9 and CXCL2 were also transiently elevated but their maximal values were by an order of magnitude lower than the values for CXCL10. These results indicate that CXCL10 is the primary inflammatory protein generated in the hippocampus in response to PIC challenge, and that this chemokine may drive the development of seizure hypersusceptibility. In addition, the hippocampus featured a protracted increase in the levels of anaphylatoxins C3a and C5a, indicating the activation of the complement cascades.

http://ift.tt/2t4Oben

[cafe-com-letra-blog]