hanbon2023

[hanbon2023]

Oligonucleotide Synthesis & Purification

Oligonucleotide synthesis is the process of chemically creating short DNA or RNA sequences in the laboratory. It involves the step-by-step assembly of nucleotides to form the desired sequence. Once the synthesis is complete, purification methods are employed to isolate and obtain pure oligonucleotides for various applications, such as research, diagnostics, and therapeutic development.

Types of Oligonucleotide Synthesis & Purification

Chromatography Column

A compact structure and simple operation and maintenance characterize the Hanbon chromatographic column.

Chromatography System

Hanbon's chromatography system offers the Bio-Lab® laboratory chromatography system, the Bio-Pro® pilot&process chromatography system, among other options, and provides dependable performance and customizable design.

Buffer Dilution/Conditioning System

It addresses the need for complex buffer solutions to be precise and repeatable, minimizes the number of storage tanks, frees up labor and material resources for dispensing, and lowers the cost of production.

Filtration System

Hanbon filtration system is suitable for biopharmaceutical customers with different scales and process routes, including Bio-TFF tangential flow filtration System, Depth filtration system, Membrane cassette holders, etc.

Oligo Synthesizer

Hanbon Sci&Tech independently invented the Bio-Oligo synthesizer, an efficient, quick, and reliable tool for oligonucleotide synthesis.

Preparative Liquid Chromatography System

The separation and purification of pharmaceutical intermediates, biopharmaceuticals, synthetic medications, natural products, fine chemicals, food additives, and other sectors make substantial use of the Hanbon preparative liquid chromatography system.

Chromatographic Column

Hanbon medium and high pressure chromatography columns include laboratory and DAC (dynamic axial compression) columns. Hanbon, which also developed the DAC® (dynamic axial compression) column in 2005, has built the largest production and assembly plant for dynamic axial compression columns.

How Is The Purity Of Synthesized Oligonucleotides Ensured?

The purity of synthesized oligonucleotides is typically assessed using techniques such as high-performance liquid chromatography (HPLC) or capillary electrophoresis (CE). These methods can separate and quantify impurities such as incomplete sequences, truncated products, and deletions. Analytical techniques like mass spectrometry can also be used to confirm the identity and molecular weight of the synthesized oligonucleotides.

[hanbon2023]

Natural Products Purification

Natural product purification is the process of isolating and purifying bioactive compounds from natural sources, such as plants, microorganisms, or marine organisms. Natural products are known for their diverse chemical structures and potential therapeutic properties. Purification aims to obtain highly pure and bioactive compounds for various applications, including drug discovery, nutraceuticals, and cosmeceuticals.

Advantages Of Using Chromatography For Natural Products Purification

Selective Separation:

Chromatography allows for the selective separation of compounds based on their physicochemical properties, such as polarity, size, and charge. This enables the isolation of specific target compounds from complex mixtures, increasing the efficiency and effectiveness of the purification process.

Versatility:

Chromatography techniques, such as column chromatography, preparative high-performance liquid chromatography (HPLC), and flash chromatography, offer a wide range of options for natural products purification. These techniques can be adapted and optimized for different compound types and purification goals.

Scalability:

Chromatography methods are scalable, meaning they can be used for small-scale laboratory purification as well as large-scale industrial production. This allows for the purification process to be easily scaled up or down based on the desired quantity of purified natural products.

High Purity:

Chromatography provides a high level of purification, resulting in the isolation of compounds with high purity. It can effectively separate impurities, such as other natural product components, contaminants, or by-products, from the target compounds, ensuring the final product is of high quality.

Advantages Of Using Chromatography For Natural Products Purification

Selective Separation:

Chromatography allows for the selective separation of compounds based on their physicochemical properties, such as polarity, size, and charge. This enables the isolation of specific target compounds from complex mixtures, increasing the efficiency and effectiveness of the purification process.

Versatility:

Chromatography techniques, such as column chromatography, preparative high-performance liquid chromatography (HPLC), and flash chromatography, offer a wide range of options for natural products purification. These techniques can be adapted and optimized for different compound types and purification goals.

Scalability:

Chromatography methods are scalable, meaning they can be used for small-scale laboratory purification as well as large-scale industrial production. This allows for the purification process to be easily scaled up or down based on the desired quantity of purified natural products.

High Purity:

Chromatography provides a high level of purification, resulting in the isolation of compounds with high purity. It can effectively separate impurities, such as other natural product components, contaminants, or by-products, from the target compounds, ensuring the final product is of high quality.

[hanbon2023]

Microbial Metabolites Purification

Microbial metabolites purification refers to the process of isolating and purifying bioactive compounds produced by microorganisms. Microbes, including bacteria, fungi, and actinomycetes, are known to produce a wide range of secondary metabolites with diverse chemical structures and biological activities.

Types of Microbial Metabolites Purification

Preparative Liquid Chromatography System

The Hanbon preparative liquid chromatography system is widely utilized in the separation and purification of pharmaceutical intermediates, biopharmaceuticals, synthetic drugs, natural products, fine chemicals, and food additives.

Chromatographic Column

Hanbon is the pioneering inventor of the DAC® (dynamic axial compression) column, introducing this technology in 2005.

Simulated Moving Bed (SMB)

In 2005, Hanbon combined the iSMB (simulated moving bed) with the DAC® (dynamic axial compression) column.

Continuous Ion Exchange Chromatography

By integrating techniques, continuous dissociation technology offers enhanced efficiency in the separation and purification processes, enabling the production of high-quality purified ingredients.

SFC (Supercritical Fluid Chromatography)

Supercritical Fluid Chromatography offers a unique and powerful approach to separation science, with broad applications in different industries and research areas.

What Are The Potential Applications Of Purified Microbial Metabolites?

Purified microbial metabolites have diverse applications in various fields, including pharmaceuticals, agriculture, food additives, and biofuels. They can serve as lead compounds for drug discovery, provide alternatives to synthetic pesticides in agriculture, act as flavor enhancers or preservatives in food products, and serve as renewable energy sources. Purified microbial metabolites have significant potential for the development of novel therapeutic agents and sustainable solutions in multiple industries.

What Are The Potential Applications Of Purified Microbial Metabolites?

Purified microbial metabolites have diverse applications in various fields, including pharmaceuticals, agriculture, food additives, and biofuels. They can serve as lead compounds for drug discovery, provide alternatives to synthetic pesticides in agriculture, act as flavor enhancers or preservatives in food products, and serve as renewable energy sources. Purified microbial metabolites have significant potential for the development of novel therapeutic agents and sustainable solutions in multiple industries.

[hanbon2023]

Insulin & Peptide Purification

Insulin and peptide purification is a critical process in the production of therapeutic peptides and proteins, particularly insulin for the treatment of diabetes. The purification aims to isolate and purify the target peptide or protein from a complex mixture of cellular components, impurities, and contaminants.

Types of Insulin & Peptide Purification

Chromatography Column

The Hanbon chromatographic column has a small overall footprint and is simple to use and maintain.

Chromatography System

Hanbon's chromatography systems can be configured in a variety of ways and have dependable performance. They include the Bio-Lab® laboratory chromatography system and the Bio-Pro® pilot & process chromatography system, among others.

Buffer Dilution/Conditioning System

This approach eliminates the requirement for complex buffer solutions to achieve accuracy and consistency, reduces the number of storage tanks needed, minimizes labor and material requirements for dispensing, and ultimately lowers the production costs.

Filtration System

The Hanbon filtration system caters to biopharmaceutical clients of various scales and process routes. It offers a range of solutions, including the Bio-TFF tangential flow filtration system, depth filtration system, membrane cassette holders, and more. These options ensure suitability and flexibility for diverse biopharmaceutical applications.

Single-use System

Our offerings include a single-use chromatography system and a single-use tangential flow filtration system. These systems feature easily replaceable contact liquid components, ensuring a fast and convenient process.

Preparative Liquid Chromatography System

The separation and purification of pharmaceutical intermediates, biopharmaceuticals, synthetic medications, natural products, fine chemicals, food additives, and other sectors make substantial use of the Hanbon preparative liquid chromatography system.

Chromatographic Column

Hanbon medium and high pressure chromatography columns include laboratory and DAC (dynamic axial compression) columns. Hanbon, which also developed the DAC® (dynamic axial compression) column in 2005, has built the largest production and assembly plant for dynamic axial compression columns.

Can Insulin Be Purified From Animal Sources?

Yes, insulin can be purified from animal sources, such as porcine (pig) or bovine (cow) pancreas. Animal pancreases contain insulin in their beta cells. The purification process involves extracting the pancreatic tissue and then isolating and purifying the insulin from other pancreatic components.

The purification steps typically include techniques such as solvent extraction, chromatography, and crystallization. Solvent extraction helps separate the insulin from other pancreatic proteins and components. Chromatography techniques, such as reversed-phase chromatography or ion exchange chromatography, can be employed to further purify the insulin and remove impurities. Finally, the purified insulin can be crystallized to obtain a highly pure form.

It's worth noting that the use of animal-derived insulin has decreased over the years with the advent of recombinant DNA technology, which allows for the production of insulin through genetically engineered organisms. Recombinant insulin is now the primary source of insulin for therapeutic use due to its purity, consistency, and reduced risk of transmitting diseases associated with animal-derived insulin.

[hanbon2023]

Chiral Separation

Chiral separation is the process of separating enantiomers, which are mirror-image isomers of a molecule that cannot be superimposed onto each other. Enantiomers possess identical physical and chemical properties but exhibit different biological activities. Chiral separation is crucial in fields such as pharmaceuticals, agrochemicals, and fine chemicals, where the specific enantiomeric form of a molecule may have different effects or interactions in biological systems.

Types of Chiral Separation

Preparative Liquid Chromatography System

The Hanbon preparative liquid chromatography system finds widespread application in the separation and purification of a diverse range of substances, including pharmaceutical intermediates, biopharmaceuticals, synthetic drugs, natural products, fine chemicals, and food additives.

Chromatographic Column

Hanbon offers a range of medium and high-pressure chromatography columns, including laboratory columns and DAC (dynamic axial compression) columns.

Simulated Moving Bed (SMB)

In 2005, Hanbon introduced the innovative DAC® (dynamic axial compression) column, which incorporates iSMB (simulated moving bed) technology.

Continuous Ion Exchange Chromatography

Continuous dissociation technology is a powerful combination of standard fixed resin bed technology and continuous counter-current systems.

SFC (Supercritical Fluid Chromatography)

Supercritical Fluid Chromatography (SFC) is a powerful chromatographic technique that utilizes supercritical fluids as the mobile phase.

How Does Chiral Chromatography Work?

Chiral chromatography relies on the use of a chiral stationary phase, which is a solid or liquid material capable of differentiating between enantiomers. The chiral stationary phase interacts selectively with the enantiomers, leading to differences in their retention times or elution orders. This separation is based on the differences in their molecular interactions, such as stereoselective adsorption or formation of inclusion complexes.

[hanbon2023]

Professional Pharmaceutical Purification Solutions

Pharmaceutical purification solutions refer to the processes and technologies used in the pharmaceutical industry to purify substances and ensure their safety, efficacy, and compliance with regulatory standards. These solutions are crucial in removing impurities, contaminants, and unwanted substances from raw materials, intermediates, and final products.

Biopharmaceutical Purification

Whether you are a user of antibodies, vaccines, recombinant proteins, mRNA, IVDs or blood products, you will find the right purification equipment.

CGT Purification

Hanbon provides Single-use chromatography systems and filtration systems for cell therapy and gene therapy; full process solutions for oligonucleotide drugs from synthesis to purification.

Small Molecule Purification

Hanbon provides purification solutions for synthetic and natural products using techniques such as atmospheric pressure chromatography, pressurized chromatography, continuous chromatography, and supercritical fluid chromatography.

[hanbon2023]

Biopharmaceutical Purification

Biopharmaceutical purification involves the separation and purification of therapeutic proteins, antibodies, and other biological molecules derived from living organisms. The process aims to remove impurities, such as host cell proteins, DNA, endotoxins, and aggregates, to ensure the safety and efficacy of the final biopharmaceutical product.

How Does Chromatography Work In Biopharmaceutical Purification?

Chromatography is a widely used technique in biopharmaceutical purification. It involves the separation of molecules based on their physicochemical properties, such as charge, size, or affinity for a specific ligand. The mixture is passed through a column containing a stationary phase, and the target molecule(s) selectively bind to the stationary phase while the impurities pass through. The target molecule(s) can then be eluted from the column for further processing.

[hanbon2023]

Antibody Purification

Antibody Purification

The purified antibodies can then undergo additional processing steps, including concentration, buffer exchange, and sterile filtration, to obtain the final product ready for formulation, characterization, and subsequent use in various applications.

Types of Antibody Purification

Chromatography Column

A compact structure and simple operation and maintenance characterize the Hanbon chromatographic column.

Chromatography System

Hanbon's chromatography system offers the Bio-Lab® laboratory chromatography system, the Bio-Pro® pilot&process chromatography system, among other options, and provides dependable performance and customizable design.

Buffer Dilution/Conditioning System

It addresses the need for complex buffer solutions to be precise and repeatable, minimizes the number of storage tanks, frees up labor and material resources for dispensing, and lowers the cost of production.

Filtration System

Hanbon filtration system is suitable for biopharmaceutical customers with different scales and process routes, including Bio-TFF tangential flow filtration System, Depth filtration system, Membrane cassette holders, etc.

Single-use System

We offer single-use tangential flow filtration and chromatography systems, both of which can have their contact liquid components quickly and easily replaced.

How is the purity of purified antibodies assessed?

The purity of purified antibodies is typically assessed using analytical techniques such as SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), capillary electrophoresis, size exclusion chromatography, or high-performance liquid chromatography (HPLC). These methods help confirm the absence of impurities and determine the purity and integrity of the antibodies.