#six1

[myfeeds]

Basic toolkit for organ development is illuminated by sea star

Despite its fundamental importance, the general mechanisms of hollow tube formation during embryogenesis are not well understood, due to the great diversity of strategies that animals use to form tubular structures. Enter the sea star, an ancient marine creature whose process of tubulogenesis is relatively easy to study, and which is becoming an important organism for understanding the genetics and mechanics of tube formation. In the May 9 issue of Nature Communications, Margherita Perillo of the Marine Biological Laboratory (MBL) and collaborators reveal in detail the initiation and early stages of tube formation in the sea star Patiria miniata. “Most of our organs are tubular, because they need to transport fluids or gases or food or blood. And more complex organs like the heart start as a tube and then develop different structures. So, tubulogenesis is a very basic step to form all our organs,” Perillo said. Perillo chose the sea star as a research organism “because I wanted to understand the basic mechanism of tube formation that is conserved across all vertebrates. So I needed an animal that was at the base along the tree of life, [evolving] before the chordates,” she said. Using CRISPR and other techniques to analyze gene function, as well as long time-lapse movies of the developing sea star larvae, Perillo and colleagues ascertained how this organism generates tubes that branch out from its gut. Her study defines a basic toolkit from which the chordate tubular organs may have developed. (Chordates include the vertebrates — fish, amphibians, reptiles, birds and mammals — and a few invertebrate subphyla). One open question in biology was exactly how organisms develop from one cell into the complex 3D tubular structures of various organs, Perillo said. In some organisms such as flies, “there is a big round of cell proliferation before all the cells start to make very complex migration patterns to elongate, change their shapes, and become a tube,” she said. In other animals, including mammals, cell proliferation and migration occur together. In the case of the sea star, “I found that, in order for tube formation, cells can proliferate and migrate at the same time,” as they do in vertebrate development. “So, this means that this mechanism of making organs was already established at the base” or root of the evolution of chordates, she said. In addition to providing insights into the fundamental process that leads to organ formation, sea stars can serve as a model for much biomedical research, Perillo suggests. For example, she found that a gene called Six1/2 serves as a key regulator of the branching process in tube formation. In mice, knocking out Six1/2 causes kidneys to form abnormally. But researchers have found that mice lacking this gene also resist tumor formation, even when injected with tumor cells. The gene, which is overexpressed in cancer cells, could lead to new ways of studying disease progression, including cancer. “I can now use this gene to understand not only how our organs develop, but what happens to organs when we have a disease, especially cancer,” she says. “My hope is that, in five to 10 years maximum, we will be able to use this gene to test how organs develop cancer and how cancer becomes metastatic.” Sea star embryos have many practical experimental advantages, Perillo said. They are mostly transparent, so internal growth processes can be directly observed over long developmental periods without harming the organism. They are also easy to collect and breed in large numbers year-round, “so I always have a lot of material to work with.”

[taylormade226]

#six1fam #fam #six1 #boxerbriefs #frenchtipnails #nomakeup #sportsbra #sportsbrasquad #whiteboy #whiteboygwala #gwala #nofucks #nofucksgiven #nofuckstogive #whiteboyrapper #whiteboyrappers #whiteboyrapperswag #swag #swaggalicious #clout #cloutchasers #cloutgang #cloutclique #letzride #standoutnation (at Oak Cliff, Dallas) https://www.instagram.com/p/B6MBcb2hiKpeRawUhjGZOuzs3TszdWocK3xsJM0/?igshid=1qhq4shqp86i

[royricephotography]

"I wish I was a radio song, the one that you turned up." Eddie Vedder royricephotography.com / iPhone SE / August 3, 2018 #six1 #pearljam #mymorningjacket #poster #nyc #seattle #airportart #travelphotography #wanderlust #neverstopexploring #throughthelensofaniphone #casacadia #royricephotography (at Seattle-Tacoma International Airport (Sea-Tac)) https://www.instagram.com/p/ByOTDuBhdX5/?igshid=of89gnwc9g22

[sixiconics]

. #sixcoupdefoudre #ilhadesãomiguel #leathergoods #leatherbag #journey #six1 #portgal (São Miguel Arcanjo, Azores, Portugal) https://www.instagram.com/p/ByHKzxhlSDU/?igshid=1w9cx0i9tmlq1

[hongism]

cal i think we discussed this before but im like 6 inches taller than u 😳

SIX1/?!?!??! WHAT DO YOU EAT TO BE SO TALL IM 😭😭

listen i lived my life in a LIE for like 4 years bc my doctor told be ‘oh yes you are 5′4!!’ then i went to the doctor at my college and they said ‘and ur 157 cm!! 5′1!!!’ i was like HUH IM WHAT

[replikavintage]

#1997 #visionaire #reikawakubo #commedesgarcons #six1/4 #paperdress #limitededitions #🖤 (at Replika Vintage)

[biomedicool]

Myogenesis

[how mature muscle cells are made]

Muscle fibers generally form the fusion of myoblasts into multi-nucleated fibers called myotubes. 

In early development, myoblasts can either proliferate, or differentiate into a myotube. 

once differentiated, myoblasts align 

cells fuse -  presence of calcium ions is critical, involves recruitment of actin to the plasma membrane, followed by close apposition and creation of a pore that subsequently rapidly widens.

Stages and associated genetic factors

Delamination --> PAX3, c-Met

Migration --> c-met/HGF, LBX1

Proliferation --> PAX3, c-Met, Mox2, MSX1, Six1/4, Myf5, MyoD

Determination -->Myf5 and MyoD

Differentiation --> Myogenin, MCF2, Six1/4, MyoD, Myf6

Specific Muscle Formation --> Lbx1, Meox2

Satellite Cells --> PAX7

[bpod-bpod]

Corti on Camera

The organ of Corti is a swirling funnel of tissue inside the ear. A spiral of auditory hair cells, highlighted in red in these mice organs of Corti (with other cells stained blue) helps to soaks up sound. The delicate hairs sway about in noise, turning audio signals into mechanical ones, then into electrical impulses bound for the brain. This dazzling setup is prone to damage – loud noises, disease and developmental problems can destroy hair cells, and they cannot regenerate. The organ on the right shows the effects of removing a single gene, Six1 from the genetic makeup of the developing ear. Instead of having strong bands of hair cells (like the healthy tissue on the left), the organ without Six1 has a broken spiral with fewer hairs. Learning more about how Six1 helps hair cells emerge from stem cells during development could influence future treatments to restore hearing later in life.

Written by John Ankers

Image from work by Ting Zhang and colleagues

Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA

Image originally published under a Creative Commons Licence (BY 4.0)

Published in PLOS Genetics, September 2017

You can also follow BPoD on Instagram, Twitter and Facebook

[moleculardepot]

LncRNA CRNDE promotes hepatocellular carcinoma progression by upregulating SIX1 through modulating miR-337-3p. A new interesting article has been published in J Cell Biochem. 2019 May 17. doi: 10.1002/jcb.28894. and titled:

[hiepmocuilga]

via Dragon Ball Super Part One Two Three Four Five Six1 2 3 4 5 6 Bundle Blu Ray USA ( 33 Bids )

[venusurveys-blog]

67

jul 24th, 2017 

(using wheel decide for this lol)

Name 7 friends.

dec

d

rox

mary

pree

mils

steph

Questions.

1] Would 3 and 6 get along if they were locked in a room together for 24 hours?: probably

2] If 6 asked you out, what would you say?: uh yikes im straight and i guess shes too

3] What’s 3’s favorite band?: idk

4] Is 1 your best friend or boy/girlfriend?: no

5] Is 2 a Democrat or Republican?: idk

6] Do you know who number 6 dated last?: i dont think shes ever dated? idk

7] Have you met 7’s parents?: no but uh kinda need to so she can hang out

8] Would you kiss 4 (if you were single)?: lool id kiss my friends tbh if i was past the first kiss stage which aint happening anytime soon *sigh* i did kiss her on the cheek on friday though. oh i miss mary

9] Are you keeping a secret for 2?: yes

Individual questions.

Number one

1] What’s their name?: dec

2] Have they ever gotten into a fight?: probs

3] What’s the last show they went to?: idk

4] Who’s their best friend (if it’s you, who’s their second best friend)?: uhmm idk

5] Do you know their phone number by heart?: no

6] What’s their middle name?: i have a feeling its andrew but like idk

7] Does their mom like you?: she dont know me

8] Do they have any cute siblings?: yess

9] Do you get along with their siblings?: never met her

10] Ever met their grandma?: no

Number two

1] What’s their name?: d

2] How old are they?: 14

3] Do they want to get married someday? i think she has to

4] Favorite singer?: idk she listens to like kpop and halsey tho

5] What’s the design of their MySpace layout?: LMAOO

6] Do they have any piercings?: ears

7] Tattoos?: no

8] Would you kiss them?: on the cheek/hand. well like i said i would kiss if i was dared to etc. anyway shes my WIFE so like

9] Are they straight, gay, bi, whatever?: i dont think i should be sayin honestly idk anymore 

10] Last place you went with them?: town

Number three

1] When’s their birthday?: june 24th

2] Does their dad like you?: havent met him

3] What classes do you have with them?: 

well this year we had maths, english & pe. strong feeling we wont be together next year :(

4] Where were they born?: im not sure? she mentioned something but i cant remember. 

5] What’s their house look like?: idk

6] Are they dating anyone? Who?: no

7] What grade are they in?: going into year 10

8] Where do they work?: nowhere

9] What’s their dream job?: cosmetology or optician.

10] How long have you known them for?: 1 to 2 years im not sure when about we met

Number four

1] Do they speak any other languages? Which?: LMAOO its mary!! ya broken english and her other one which idek what it is lols

2] What’s their ethnicities?: brown

3] What’s their best school subject?: uhm...idkk lol

4] Have you ever lied to them?: yes 

5] What’s the last gift they gave you?: i dont think she ever gave me one....the bitch.....

6] What does their hair look like?: long, black, straight,shiny. ugh. i love her hair.

7] Did they go on vacation this year? Where to?: no but shes going back to her country very soon :(

8] Would you switch wardrobes with them?: we have similar style

9] Do you live within walking distance?: no. soon it will certainly not be. duBAI.

10] Do they take the bus, walk, or get another way home from school?: she went by car

Number five

1] What’s their name?: pree

2] Are they a boy or a girl?: girl

3] What’s their hair color?: black

4] What’s their worst school subject?: idk

5] Are they single?: yes

6] Do they play any instruments? Which?: i dont hink so

7] Where did you guys meet?: at school

8] What’s their view on abortion?: i assume shes  pro choice

9] What song reminds you of them?: i dont have one

10] Were they born in another country?: yes

Number six

1] What’s their name?: mils

2] Out of the other 6 people on the list, who does this person get along with?: all but dec. she doesn't know him.

3] Last time you saw them?: earlier this month

4] How tall are they?: just a few cm taller than me. like 5′1 maybe?

5] What’s their dress style?: dark, trendy, kinda instagramy

6] Favorite food? idk

7] Ever had a crush on this person?: no

8] What school do they go to?: my school

9] Ever had a dream about them? What happened?: idk

10] Do they wear contacts or glasses?: she wears fake glasses sometimes

Number seven

1] What’s their name?: steph

2] When’s their birthday?: boxing day

3] Have they ever dyed their hair?: no

4] Weirdest hairstyle they ever had?: omgggg. all her hairstyles are unique and iconic.

5] What color do they usually wear?: reds/pinks

6] How much do they weigh?: idk but she complains about being chubby so

7] Did they have a birthday party for their birthday?: i think

8] How old are they?: 14

9] What year did you meet them?: 2016

10] Did you ever get into a fight? Over what?: no. but she was in a mood with me and i with her

[beahkawaizxu]

Dragon Ball Super Part One Two Three Four Five Six1 2 3 4 5 6 Bundle Blu Ray USA ( 44 Bids ) https://t.co/THGhm4dcKA

Dragon Ball Super Part One Two Three Four Five Six1 2 3 4 5 6 Bundle Blu Ray USA ( 44 Bids ) https://t.co/THGhm4dcKA

— Beahka.Waizxu (@BWaizxu) July 5, 2019

via http://twitter.com/BWaizxu/status/1147221656030629888 from Twitter https://twitter.com/BWaizxu

[sixiconics]

#sixcoupdefoudre #banksy ? #ilhadesãomiguel #leathergoods #leatherbag #journey #six1 https://www.instagram.com/p/Bx7HnmIlmPw/?igshid=2vzxcl4s0b5k

[tuagyuzuobso]

Dragon Ball Super Part One Two Three Four Five Six1 2 3 4 5 6 Bundle Blu Ray USA ( 33 Bids ) https://t.co/hinEYRwmaA

Dragon Ball Super Part One Two Three Four Five Six1 2 3 4 5 6 Bundle Blu Ray USA ( 33 Bids ) https://t.co/hinEYRwmaA

— Tuagyu Zuobso (@tuagyu) June 28, 2019

from Twitter https://twitter.com/tuagyu June 28, 2019 at 01:56PM

[cyberianpak]

Colorado drug takes aim at cancer metastasis

Colorado drug takes aim at cancer metastasis

[ad_1]

IMAGE: Heide Ford, Ph.D., and colleagues show that targeting SIX1/EYA may stop breast cancer metastasis. view more 

Credit: University of Colorado Cancer Center

Many cancers are relatively harmless at their site of origin, and it is only when they metastasize to sites like the brain, bones, lungs, and liver that they become especially dangerous. And so, in addition to stopping the…