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Lipidomics of homeoviscous adaptation to low temperatures in Staphylococcus aureus utilizing exogenous straight-chain unsaturated fatty acids over biosynthesized endogenous branched-chain fatty acids
It is well established that Staphylococcus aureus can incorporate exogenous straight-chain unsaturated fatty acids (SCUFAs) into membrane phospho- and glyco-lipids from various sources in supplemented culture media, and when growing in vivo in an infection. Given the enhancement of membrane fluidity when oleic acid (C18:1{Delta}9) is incorporated into lipids, we were prompted to examine the effect of medium supplementation with C18:1{Delta}9 on growth at low temperatures. C18:1{Delta}9 supported the growth of a cold-sensitive, branched-chain fatty acid (BCFA)-deficient mutant at 12oC. Interestingly, we found similar results in the BCFA-sufficient parental strain. We show that incorporation of C18:1{Delta}9 and its elongation product C20:1{Delta}9 into membrane lipids was required for growth stimulation and relied on a functional FakAB incorporation system. Lipidomics analysis of the phosphatidylglycerol (PG) and diglycosyldiacylglycerol (DGDG) lipid classes revealed major impacts of C18:1{Delta}9 and temperature on lipid species. Growth at 12oC in the presence of C18:1{Delta}9 also led to increased production of the carotenoid pigment staphyloxanthin; however, this was not an obligatory requirement for cold adaptation. Enhancement of growth by C18:1{Delta}9 is an example of homeoviscous adaptation to low temperatures utilizing an exogenous fatty acid. This may be significant in the growth of S. aureus at low temperatures in foods that commonly contain C18:1{Delta}9 and other SCUFAs in various forms. http://dlvr.it/T2HJlb
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Simlish Dictionary (Dictionaries)
ENGLISH | SIMLISH
Like/Love: Luvey, Luv
I: A
You: Vous
Dislike/Hate: Benaza
Colour: Coula
Music: Musu
Milk: Muku, Lalo
Gross: Grobe
This is cool: Whippna Choba Dog
Dog: Woofum
Very Good: Ooh Be Gah
Girl: Gurl
Boy: add
In: Ba
Chat: Obray
Speak: Sperk
My: Za
Is: Es
We: Mis
Are: Bon
Fall: Fwa
F**k: Plum
This: Dis
Newborn: Nubu
S**t: Scufa
What’s Up?: Hooba Noobie?
How are you?: Cuh Teekaloo?
Interesting: Jowlenin
Cosmopolitan: Simpala
Politan/Inhabitant/Native: Pala
Bartender: Bartem
Bowl of Nuts/Oaks: Bashoga
Amore: Amoo
See you later: Geelfrob
Bumbaclot/I don’t like: Boobasnot
Start of a knock-knock joke: Oga Surpa
Add: Soun it bra
Add: Tanbannan
Add: Plamba Chamba! Porbleen!
Add: Angee
I’m on fire!: Wabadebadoo!
I love you: A Lav Vous
I need to pee: Atohteh
Disgusting: Disgrobol
Embarrassment: Aye yai yai
Bath: Splish
Rubber Duck: Rabu Doyi
Awkward: Ekowood
Clothes: Clops
Kiss: Kik
Make Out: Mik Up
Everything: Fretishe
I’d like a taxi to come pick me up: Nicloske Ga Gloope
Star Wars: Journey to Batuu DLC
ENGLISH | SIMLISH
Move Along: Maba Lag
Galaxy: Galaxsu
Sabacc: Sibaba
Lightsaber: Laksibar
References
Simlish Dictionary - Pages - Learn Simlish (Defunct)
3 Ways to Speak Simlish - wikiHow
Learn Simlish on Piczo (Defunct)
Changelog
27th December 2023: Added new words and phrases to the list
19th December 2023: Created post
#warrenwoodhouse#gaming#dictionary#dictionaries#learn#learn simlish#simlish#simlish dictionary#.dictionary#2023
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#pithkrew#noodleheadx#@polar.vertex#polar#vertex#nft#pixel gif#pixel art#anne vieux#minimalist#george#webb#neighborhood news hour#addy adds#deep state#scufa#novation supernova#akai#mpc2000xl#elecroni#fuck kanye#west virginia#donda#yeezy700#yeezy350#joe budden#trump#akira#明#カツヒロ大友
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Glad I got to see this in person! Tyler Gordon's SCUFA (Self-Contained Untethered Flameworking Apperatus) demoing on the steps outside of the student show. @juicypieceofglass #corning #gas2016 #scufa #flameworking (at 171 Cedar Arts Center)
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#SamP Book your session today ! Pay in person or pay online ! Call me now 2404403485 #ChasePaperMusicFestival2016 #June18th #BakersParkBandshell #Frederick #MD #CorporateHustlinApparel #TheLeague #WeirdLighterGang #TheCouncil #Dat301 #ChasePaperMusic #Goal #1200Money #Ateam #CrystallBabii #RubeDat #Scufa #TheNakedScoop
#thecouncil#chasepapermusicfestival2016#corporatehustlinapparel#ateam#scufa#goal#md#1200money#theleague#frederick#weirdlightergang#crystallbabii#bakersparkbandshell#june18th#rubedat#samp#thenakedscoop#dat301#chasepapermusic
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My sustainable fashion outfit!!! #scufa #SuckingInThatTummy #UsingThemStarsTOHideThingsLOL
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