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[mycochaotix]

MCX Rants: Cloning

r/ContamFam mycopal asks: “Hey pal do you have any good tips or sources for info on moving mushroom tissue to agar?”

MCX Answers: The way I understand it is: you can do two types of transfers for cloning, considered most common and effective.

One is as you mentioned the splitting stipe and taking a transfer from inside the stipe. You want to ensure that your area, yourself, and your tools are cleaned with isopropyl alcohol 70% . You will tear from the base up towards the cap, with the exposed part of the STIPE, towards your laminar flow, or towards the back of your still airbox. you only need a rice size piece of transfer.

Often the inner flesh is very stringy and breaks away quite easily. I’ll personally just cut a rice size cut in a square shape from the inner part of the stipe and then gently poke that and pull it out and put it on a plate. Sometimes inner Mycelium flesh can pull away and you can put that on a plate. The mushroom fruit body is all mycelium. Everything about it is made from mycelium, and arguably all of its components, contain but also can, either: produce or generate, and proliferate mycelium from that fruit body.

The second way involves approaching the xfer from the topside of the cap, ‘dorsal’ and cutting open the center dorsal point and then take from inside the cap at the pileus-stipe junction… but from within the cap… its a little more technical and often can expose the xfer to contam from the flesh u open up before taking xfer if you arent careful in how you peel back the external flesh and when you push the knife into the cap it can push microbes down into the previously unexposed inner mycelial tissue).

The idea for these types of transfers is that contaminants in your environment that float in around you or fall off of you, if present, likely is on or near your external fruitbody. If you take from the inside of the fruitbody its arguable that its more clean and free of contaminant microbes as the primordia formation is assumedly on healthy uncontaminated mycelium and unless the flesh tears during maturation, the inner parts are fully protected from external contaminant sources while growing.

Sometimes these cooccurring microbes are harmless to your grow and to you, and can even be beneficial (like certain yeasts that are used in agar preparation) or even some naturally occurring bacteria in the substrate! I think of my tubs as a homeostatic localized microclimate - environment :)

Our tubs, and the fungi in them have similar homeostasis in the wild, and can be conceptually applied to community mycology cultivation!

I think of comparing potential “microcosm” cultivation much like the human body, external and internal… theres always gonna be contaminant microbes on our skin and around our mucosal membranes, but with proper hygiene and diet our body is its own microcosm of microbes that are fucking and fighting away under our literal noses xD —- its when one grouping of these microbes begins to advantage over the equalized body microcosm we have (think if you dont bathe you may develop yeast and bacterial infections more easily as surface conditions of your skin and within your creases, nooks and crannies will can be overpowered by something that spreads… )

and thats kind of the basic idea of germ theory I think… and I also think it applies to our grow environments if approach cultivation holistically :)

surely im missing technical nuance but I know my thoughts are in the spirit of the theory!

Hope my thoughts aid your mycojourney!

-MCX

Related resources:

- Yoshi Cloning vid and result video

- PGT Cloning Vid

- Ashley/Boomer Shroomer Cloning vid

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Top 5 Best Stock Brokers in India

With a rise in the number of stock brokers, it has been getting difficult for people to find who is the best stock broker in India, and to clear all the confusion, we have bought a list of the top and best stock brokers in India. Since 2019, Zerodha has been ranked as the best stock broker in India with the highest number of active users on their trading platform and if you want to learn more about the best stock brokers, then keep reading this article.

What are stock brokers?

A stock broker is a highly skilled and professional trader who performs trading activities on behalf of his clients. Stock brokers are also known as financial and investment advisors. Nowadays, a high number of stock brokers work in a brokerage firm and handle multiple stocks and trades for their clients.

What is the role of stock brokers?

Buying and selling stocks and shares on behalf of clients or investors.

Do extensive market research and Advise investors on financial securities and buying and selling stocks.

Managing the investment portfolios of the investors.

Handling and management of all the paperwork regarding financial investments.

Creation of strategies depending upon market conditions and changes.

What are the two types of best stock brokers in India?

There are usually only two types of stock brokers:

Full-Service Stock Brokers:

As the name says, full-service stock brokers provide full service to their clients. They usually charge a higher fee because of their vast benefits, like advice, research, portfolio management, etc. full-service stock brokers charge brokerage as a commission on the total amount of trade.

Discount Stock Brokers:

List of Best Stock Brokers in India

Zerodha.

Upstox.

Angel Broking.

ICICI Direct.

5Paisa.

Zerodha

Zerodha topped our list as India’s number-one stockbroker with more than one crore active clients. It is the most preferred platform for beginners and advanced traders due to its clean user experience and robust technology.

Zerodha Kite is the flagship level trading platform from Zerodha, which is available on both desktop and mobile.

Some of the most remarkable features of the Zerodha platform are:

Free equity delivery trading with zero brokerage fee.

On-time order update push notifications through their app.

Multiple chart types to help you evaluate market conditions.

No commission charges on investment in mutual funds.

Minimalistic and clean interface.

Advantages and Disadvantages of Zerodha:

Pros:

Most trusted stock broker in India.

Zero brokerage charges.

The app is available for both android and IOS.

No paperwork is required for account opening.

Cons:

A yearly account maintenance fee of ₹300 has to be paid.

Doesn’t offer any free stock tips in comparison to other platforms.

Upstox

Upstox is a discount broker platform, the second largest broker in India after Zerodha. It is backed by an eminent personality like Mr. Ratan Tata and a top investment firm: tiger global management.

Some of the top notch features of Upstox are:

Allows investment in gold and IPOs.

Easily apply more than a hundred technical indicators on real-time charts.

Sends push notifications about the current prices of your favorite stocks.

Upstox is a member of major stock markets i.e. NSE, BSE, and MCX-SX.

Advantages and Disadvantages of Upstox:

Pros:

Brokerage charges are fair and reasonable.

The platform is available for android, IOS, and Windows.

No account maintenance or opening charges.

Easy to understand charts.

Cons:

Some bugs and glitches in the latest version of the app.

Call and trade orders are charged at ₹20 per trade.

The web version of the platform is sophisticated.

Angel Broking

Angel broking was founded in 1987 and had a customer base of more than 2 million active traders. With that, it ranked number three in our list of the top 5 best stock brokers in India.

Some of the most outstanding features of Angel Broking are:

You choose from a list of readymade portfolios.

Over 40 technical chart indicators and overlays.

Easily invest in international stocks.

View demat account holdings from the app.

Advantages and Disadvantages of Angel Broking:

Pros:

Reliable trading platform.

An extensive network of sub-brokers.

Excellent customer care service.

Advanced trading platform.

Cons:

Doesn’t offer three in one account.

Their sales team will irritate you by trying to cross-sell their products.

Comparatively higher charges than competitors.

ICICI Direct

ICICI Direct is one of the largest and oldest stock brokers in India, a part of ICICI Bank, one of the most prestigious private sector banks in India. With a vast customer base of more than 5 million, it effectively offers a clutter-free and smooth online trading and investment experience.

Some of the best features of ICICI Direct are:

Provides detailed research across equity and derivatives.

Identify market behavior using heatmaps.

Biometric login.

Multiple plans and features are available as per the person’s needs.

Advantages and Disadvantages of ICICI Direct:

Pros:

Highly responsive 24/7 customer care service.

Real-time data and information.

3 in 1 demat account.

Highly secure.

Cons:

The login process is extremely slow and cluttery.

Not a beginner-friendly interface.

The mobile app needs much more improvement.

5Paisa

5Paisa has recently gained immense popularity and has become one of the most popular online trading platforms in India. It acquired more than 10 lakh customers in a short period of just four years because of the enhanced user experience it provides to its customers.

Some of the best features of 5Paisa:

With 5Paisa, you can invest in U.S. stocks.

Set price alerts and get notifications through their app.

Clean U.I. and UX.

Advanced tools and charts for advanced technical analysis.

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